Y-27632 2HCl

目录号:S1049

Y-27632 2HCl Chemical Structure

Molecular Weight(MW): 320.26

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

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RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户购买Selleck的此次产品后发表的文献55篇:

客户使用该产品的6个实验数据:

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    At day 8, cells were replated onto Matrigel-coated Transwell membranes at 106 cells/cm2 in the presence or absence of 10 mM Y-27632. TEER was measured at day 10, 2 days after replating.

    Science, 2017, 3(11):e1701679. Y-27632 2HCl purchased from Selleck.

  • YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells MoqxSpVv[3Srb36gRZN{[Xl? NITqXWsyOCEQvF2= Mnv0NkBp MnrjSG1UVw>? NY[zOWJlUW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? NGTM[VA6PjR5NkW0
N1E-115 NG\zfWpHfW6ldHnvckBCe3OjeR?= M2nOTVExKM7:TR?= NHfEUHYzKGh? NWLmd3Z1TE2VTx?= NX3jfm96UW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? MlPBPVY1PzZ3NB?=
HeLa MW\GeY5kfGmxbjDBd5NigQ>? MlLwNVAh|ryP MVezNEBucW5? NY\zVodrUW6qaXLpeJMhfGinIH\vdo1ifGmxbjDv[kB{fHKnc4Og[olj\XK|IHHu[EB1cGViYYPz[Y1jdHlib3[geolv[3WuaX6tZ49vfGGrbnnu[{Bnd2OjbDDh[Ihme2mxboO= MlX6PVY3QDB5Mh?=
CCL39 NFTYPVZHfW6ldHnvckBCe3OjeR?= MWqzNEDPxE1? MUizNEBucW5? M3jtVmNwdXCuZYTlcJkh[WKxbHnzbIV{KGGldHn2ZZRqd25ib3[gUoEuUCCneHPoZY5o\XJiTljFNUBjgSCrboTl[5JqdnN? M{jKWFk3QTN|OEK=
Mesothelial cells from rat mesentery MkP0TY53[XOrdnWgRZN{[Xl? MWKzNEDPxE1? MWWyNEBp MmLqRoxw[2u|IHnueoF{cX[nIHHjeIl3cXS7 NGXldWc6QTNyOEey
NIH3T3 NV3NeGQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH:yN2QyOCEQvF2= M1LXPFE5KGR? MlLBSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NHfVd4wyODB{MUO4Oi=>
Dbl-d NIDwfVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHvTox7OTBizszN NHPPbHkyQCCm NWXkWlJRW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NGfSeGcyODB{MUO4Oi=>
Dbl-e NUjxdI5{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHzNVAh|ryP NUWzdFB7OThiZB?= M1HWe21w\GW{YYTlcJkhcW6qaXLpeJMh[2WubDDndo94fGh? M4\2fFExODJzM{i2
mNET1-d MlfiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrv[G84OTBizszN NWDmdWJHOThiZB?= M1rrUnN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NV7FdXNmOTByMkGzPFY>
mNET1-e MmjDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4q0SlExKM7:TR?= MXuxPEBl Ml71V5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> M{XLPFExODJzM{i2
Ras-2 NHXkZ4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3u0ZlExKM7:TR?= NGPibYYyQCCm NYO2b2RwW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NGqwU4kyODB{MUO4Oi=>
Ras-4 NYK5[VJUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NES2ZZoyOCEQvF2= MXOxPEBl NGmy[nRUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? NFLkdGgyODB{MUO4Oi=>
Src-1 M4C1cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrwTHoyOCEQvF2= NWPvcGw1OThiZB?= M3KyUWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MmnjNVAxOjF|OE[=
Src-4 NFy4PVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MofqNVAh|ryP M{P3elE5KGR? NFvnSY9Fd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MmPvNVAxOjF|OE[=
NIH3T3 M4LFR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXnpZpl1OTBizszN MXqxPEBl MVzEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MXWxNFAzOTN6Nh?=
Src-1 M13kNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWixNEDPxE1? MUmxPEBl NWPyO4duTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M{f0XFExODJzM{i2
Src-2 M4PVfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTsTVYyOCEQvF2= NV7pbWlROThiZB?= NH[wOlhFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MmPxNVAxOjF|OE[=
SW620 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjOOVROOTBizszN NIC1VokyQCCm Mn23SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NFuzPJoyODB{MUO4Oi=>
HCT15 NWKwfVFGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUGxNEDPxE1? NGrBRY0yQCCm MUnEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To Mn3xNVAxOjF|OE[=
HCT116 MkLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWGxNEDPxE1? M{n4[VE5KGR? NYnlVIwxW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NVLFWpVSOTByMkGzPFY>
LS174T M2DYNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fz[VExKM7:TR?= MkTSNVgh\A>? NYf1bJJ1VW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= NEjxfYQyODB{MUO4Oi=>
Neonatal rat ventricular myocytes MYjGeY5kfGmxbjDBd5NigQ>? NH\ZT5YyOCEQvF2= NUHJeXF[PDhiaB?= NFXI[WFKdmirYnn0d{BGXC1zLXnu[JVk\WRiaX7jdoVie2W|IHnuJJBzd3SnaX6gd5lvfGinc3nzMEBk\WyuIIPpfoUh[W6mIH35c4Zq[nKrbHzhdkBwemejbnn6ZZRqd25? M3HOWVExOzh4NkGz
Stellate Cell MknPSpVv[3Srb36gRZN{[Xl? NV;Vb4FoOjVizszN MnfFNVUhdWmw MoLnTY5pcWKrdIOg[o9zdWG2aX;uJI9nKEZvYXP0bY4he3S{ZYPzJIZq[mW{czDhcoQheGixc4Doc5J6dGG2aX;uJI9nKG27b4PpckBtcWeqdDDjbIFqdg>? MXKxNFYxODR7Nh?=
Rat Vascular Smooth Muscle Cells MlqwSpVv[3Srb36gRZN{[Xl? NE\EWYYyOCEQvF2= M{njNVIhcA>? NIq3WVNKdmirYnn0d{Bidmerb4TlcpNqdiCLST3pcoR2[2WmIHj5dIVzfHKxcHj5 NIj6S3YyODZ2MkOxOy=>
PC3 M37VO2Z2dmO2aX;uJGF{e2G7 NWDR[pY3OjVizszN MVWxJIg> MWXJcoR2[2W|IH3vdpBpd2yxZ3njZYwh[2ijbnfldy=> NF3qNosyODd{MES3NS=>
PC3 NX;Dc4RyVWmpcnH0bY9vKEG|c3H5 MVOyOUDPxE1? MV2xJIg> MlvlTY5pcWKrdIOgeIhmKEKPRlKtR20h[W6mIITo[UBGT0Zvc4TpcZVt[XSnZDDtbYdz[XSrb36= Mme1NVA4OjB2N{G=
PC3 M1XqUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjENlUh|ryP NFjoWVAyPyCq MYDEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NWnyT29wOTB5MkC0O|E>
LNCaP NIDmZnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fXZ|I2KM7:TR?= MWOxO{Bp NWLmRpFjTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? NHTEN|IyODd{MES3NS=>
Rat hepatic stellate cells NFzre2ZHfW6ldHnvckBCe3OjeR?= NVPIU|dwOzBizszN MV:0PEBp M2q4fWRqdWmwaYPo[ZMhfGinIIDoc5NxcG:{eXzheIlwdiCxZjDFdoszNCCjbnSg[IVkemWjc3XzJI5mfyCGTlGgd5lvfGinc3nz NH3JdmUyODh2NU[2Ny=>
Pancreatic acinar cells MV7GeY5kfGmxbjDBd5NigQ>? M4jVNFExyqEQvF2= MUC3NEBucW5? NXvRVoprWG:2ZX70bYF1\XNiQ1PLMZN1cW23bHH0[YQheGGwY4LlZZRq[yCnbor5cYUhe2WlcnX0bY9v NITtPHYyOjd2NUC4NC=>
C2C12 MX7GeY5kfGmxbjDBd5NigQ>? MUixNOKh|ryP NGXGUI03KGh? M3HYT3Bz\X[nboTzJJRp\SC|ZYLpcoUheGixc4Doc5J6dGG2aX;uJI9nKEmUUz2xJIlv\HWlZXSgZpkhcW6|dXzpckBidmRxb4KgWG5HNc7z MXixOlI3PzF{NB?=
PC 12 NHjZUWNHfW6ldHnvckBCe3OjeR?= MVWxNOKh|ryP M3P4PVI1KGh? MWDBeJRmdnWjdHXzJINifGWlaH;sZY1qdmViYnnvd5lvfGinc3nz NF7je4IyPjJzOUSyOC=>
Cynomolgus monkey embryonic stem cells MVXDfZRwfG:6aXOgRZN{[Xl? MkHUNlAhyrWP NYfrU4lEOjRiaB?= M12zVXBzd22xdHXzJIN6TVNiY3XscEB{fXK4aY\hcC=> M1uyd|E5QTRyOEW1
TSGH 8301 M1niR21q\3KjdHnvckBCe3OjeR?= M2[5TVIxKML3TR?= NHXtZokyKGh? MXLJcoNz\WG|ZYOgZ4VtdCCvaXfyZZRqd25? M3PDPVE6QDl4NEe1
Swiss3T3 NFnCUWlEd2yxbomt[o9zdWmwZzDBd5NigQ>? Mnv4NVAhyrWP NVjOTFVpOTNiZB?= M2\qNGlv[3KnYYPld{Bxem:|dHH0[UBk\WyuIHPvcI9vgS2ob4LtbY5oKGGldHn2bZR6 MmG4NlE1PjR7MEK=
HT22 MmTIR5l1d3SxeHnjJGF{e2G7 M1niWlExKML3TR?= NWnrNZdTOTNiaB?= MkHCVJJwfGWldIOgZYdicW6|dDDncJV1[W2jdHWtbY5lfWOnZDDu[ZVzd26jbDDk[YF1cA>? MoDhNlI5OTB6M{W=
Salivary gland stem cells NEi0cndHfW6ldHnvckBCe3OjeR?= NVnQW4t6OTBiwsXN MYS3JIQ> NFLFeJZT\WS3Y3XzJHNIW0Nic3Xu[ZNk\W6lZR?= M3e3VFI2QDB2NU[w

... Click to View More Cell Line Experimental Data

体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠,肾性高血压大鼠,及去氧皮质酮醋酸(DOCA)盐高血压大鼠,显著降低血压,这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠,持续11天,延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环,通过抑制ROCK, 降低 缺氧诱导的血管生成,和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1] [7]
+ 展开
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Formulation: 溶于DMSO,然后在盐水中稀释 (大鼠); 0.9% NaCl (小鼠)
  • Dosages: 30 mg/kg/day (大鼠);0-10 mg/kg (小鼠)
  • Administration: 口服处理 (大鼠);ip(小鼠)
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
saline
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl

CAS号 129830-38-2
稳定性 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 问题 2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

相关ROCK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID