Y-27632 2HCl

目录号:S1049

Y-27632 2HCl Chemical Structure

Molecular Weight(MW): 320.26

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

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RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户购买Selleck的此次产品后发表的文献53篇:

客户使用该产品的6个实验数据:

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    At day 8, cells were replated onto Matrigel-coated Transwell membranes at 106 cells/cm2 in the presence or absence of 10 mM Y-27632. TEER was measured at day 10, 2 days after replating.

    Science, 2017, 3(11):e1701679. Y-27632 2HCl purchased from Selleck.

  • YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells NGDEXJhHfW6ldHnvckBCe3OjeR?= NEf0O3YyOCEQvF2= M2PzOlIhcA>? MVzEUXNQ MYnJcohq[mm2czD0bIUh[XO|ZX3icJkhd2ZibXnjdo91fWK3bHXzJIFv\CCrboTldo1m\GmjdHWg[olt[W2nboTzJJRwKG[xcn2g[Zh1\W6mZXSgdJJw[2W|c3Xz NWOxeYVLQTZ2N{[1OC=>
N1E-115 M1XBWGZ2dmO2aX;uJGF{e2G7 Mlr4NVAh|ryP MWKyJIg> M3LPbGROW09? NUnaZ2V1UW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? MlznPVY1PzZ3NB?=
HeLa MoLQSpVv[3Srb36gRZN{[Xl? M3exN|ExKM7:TR?= M3vablMxKG2rbh?= M1vWcmlvcGmkaYTzJJRp\SCob4LtZZRqd25ib3[gd5Rz\XO|IH\pZoVzeyCjbnSgeIhmKGG|c3XtZox6KG:oII\pcoN2dGmwLXPvcpRicW6rbneg[o9k[WxiYXTo[ZNqd26| MYO5OlY5ODd{
CCL39 MlrSSpVv[3Srb36gRZN{[Xl? MkixN|Ah|ryP NXixUnAxOzBibXnu Mn;oR49ueGyndHXsfUBi[m:uaYPo[ZMh[WO2aY\heIlwdiCxZjDOZU1JKGW6Y3jhcodmeiCQSFWxJIJ6KGmwdHXndolvew>? NFnQRmQ6Pjl|M{iy
Mesothelial cells from rat mesentery NUjNXWxZUW64YYPpeoUhSXO|YYm= Ml30N|Ah|ryP MnTPNlAhcA>? NFvYPGJDdG:la4OgbY53[XOrdnWgZYN1cX[rdIm= M{\zSFk6OzB6N{K=
NIH3T3 NVfZT2NRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jzZ|ExKM7:TR?= MWSxPEBl NHzEdo5Fd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp M13neVExODJzM{i2
Dbl-d M1vCWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoD6NVAh|ryP MmruNVgh\A>? MUfTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NU\OXZdJOTByMkGzPFY>
Dbl-e M37T[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3pRlQyOCEQvF2= M{j6NVE5KGR? MoHXUY9l\XKjdHXsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? MnvuNVAxOjF|OE[=
mNET1-d MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnrNVAh|ryP MkixNVgh\A>? Mmm4V5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> M{PWSVExODJzM{i2
mNET1-e NXLKNJRET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXEXFBHOTBizszN NHewUJcyQCCm MWHTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> Mlz3NVAxOjF|OE[=
Ras-2 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXGU|YyOCEQvF2= NE\QSXMyQCCm NV7Ob4E3W3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? M13UZlExODJzM{i2
Ras-4 M1fpR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4K0NlExKM7:TR?= NXzTW2RiOThiZB?= MVzTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> Mn;jNVAxOjF|OE[=
Src-1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fx[|ExKM7:TR?= Ml7LNVgh\A>? NGL5[IhFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MlXmNVAxOjF|OE[=
Src-4 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYmzcWJ[OTBizszN NVj4c|VGOThiZB?= MUPEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NGHlUGgyODB{MUO4Oi=>
NIH3T3 MlTnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVP2eFgyOTBizszN NVrofYNDOThiZB?= M1WzV2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NF[4WHkyODB{MUO4Oi=>
Src-1 MnPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\rNVAh|ryP NVi0NHhzOThiZB?= NVvRcnpjTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? NEHYdmcyODB{MUO4Oi=>
Src-2 NGn2fFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFvlSFQyOCEQvF2= MkfENVgh\A>? NHj2cHlFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NV;6UGRUOTByMkGzPFY>
SW620 MkD2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2L5e|ExKM7:TR?= MoTQNVgh\A>? MYHEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To M1TYNlExODJzM{i2
HCT15 NEKyVZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4i3V|ExKM7:TR?= M3PmblE5KGR? NV7oOWVCTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MW[xNFAzOTN6Nh?=
HCT116 NETnUnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX[xNEDPxE1? MX6xPEBl NUjGZ2k5W3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? MUmxNFAzOTN6Nh?=
LS174T MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTPU41KOTBizszN MYSxPEBl NUP6dng1VW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= MXWxNFAzOTN6Nh?=
Neonatal rat ventricular myocytes MojYSpVv[3Srb36gRZN{[Xl? MUOxNEDPxE1? NITac3I1QCCq Ml7xTY5pcWKrdIOgSXQuOS2rbnT1Z4VlKGmwY4LlZZNmeyCrbjDwdo91\WmwIIP5cpRp\XOrczygZ4VtdCC|aYrlJIFv\CCveX;mbYJzcWyuYYKgc5Jo[W6renH0bY9v NEHDVGcyODN6Nk[xNy=>
Stellate Cell NX76empQTnWwY4Tpc44hSXO|YYm= NGi1d2gzPSEQvF2= NWLEPHp[OTVibXnu NUPuUo9WUW6qaXLpeJMh\m:{bXH0bY9vKG:oIF[tZYN1cW5ic4Ty[ZN{KG[rYnXyd{BidmRicHjvd5Bpd3K7bHH0bY9vKG:oIH35c5NqdiCuaXfoeEBkcGGrbh?= MUWxNFYxODR7Nh?=
Rat Vascular Smooth Muscle Cells M4rlOmZ2dmO2aX;uJGF{e2G7 MmW1NVAh|ryP NGrNdIgzKGh? M2TiUWlvcGmkaYTzJIFv\2mxdHXud4lvKEmLLXnu[JVk\WRiaInw[ZJ1em:yaIm= NHznVowyODZ2MkOxOy=>
PC3 M1:xOmZ2dmO2aX;uJGF{e2G7 MWiyOUDPxE1? NF6xPYoyKGh? MlvwTY5lfWOnczDtc5JxcG:ub3fpZ4FtKGOqYX7n[ZM> NGfVT|gyODd{MES3NS=>
PC3 MUDNbYdz[XSrb36gRZN{[Xl? M{XqNlI2KM7:TR?= NVHFfIJ2OSCq NHv2T|lKdmirYnn0d{B1cGViQl3GRk1EVSCjbnSgeIhmKEWJRj3zeIlufWyjdHXkJI1q\3KjdHnvci=> MlfqNVA4OjB2N{G=
PC3 MliyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXsUY0zPSEQvF2= NEnJZmQyPyCq MVvEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MkXpNVA4OjB2N{G=
LNCaP NUTPZo01T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfzfVk5OjVizszN NVXR[YxUOTdiaB?= MWrEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NGfROYoyODd{MES3NS=>
Rat hepatic stellate cells M1vVcWZ2dmO2aX;uJGF{e2G7 NHTZUHE{OCEQvF2= M2LoZlQ5KGh? NHjIdZdFcW2rbnnzbIV{KHSqZTDwbI9{eGixconsZZRqd25ib3[gSZJsOixiYX7kJIRm[3KnYYPld{Bv\XdiRF7BJJN6dnSqZYPpdy=> MknWNVA5PDV4NkO=
Pancreatic acinar cells NVLo[5ZrTnWwY4Tpc44hSXO|YYm= M{nYO|ExyqEQvF2= M1T3elcxKG2rbh?= NFLScllRd3SnboTpZZRmeyCFQ1utd5RqdXWuYYTl[EBx[W6lcnXheIlkKGWweont[UB{\WO{ZYTpc44> MnvBNVI4PDVyOEC=
C2C12 MkXhSpVv[3Srb36gRZN{[Xl? NHLvfFUyOMLizszN NXS3e|lZPiCq NVL1TZFWWHKndnXueJMhfGinIIPldolv\SCyaH;zdIhwenmuYYTpc44hd2ZiSWLTMVEhcW6mdXPl[EBjgSCrboP1cIlvKGGwZD;vdkBVVkZvzsG= MmqzNVYzPjdzMkS=
PC 12 NInGVIJHfW6ldHnvckBCe3OjeR?= Mlf5NVDDqM7:TR?= NV3iUXBROjRiaB?= M1GwUWF1fGWwdXH0[ZMh[2G2ZXPoc4xidWmwZTDibY9{gW62aHXzbZM> NF\0No8yPjJzOUSyOC=>
Cynomolgus monkey embryonic stem cells M2jTW2N6fG:2b4jpZ{BCe3OjeR?= MVyyNEDDvU1? Mnf2NlQhcA>? Ml7pVJJwdW:2ZYOgZ5lGWyClZXzsJJN2en[rdnHs M2XZeVE5QTRyOEW1
TSGH 8301 NV2wPYxUVWmpcnH0bY9vKEG|c3H5 NH3neoozOCEEtV2= NGnIcpcyKGh? M{TJRmlv[3KnYYPld{Bk\WyuIH3p[5JifGmxbh?= NYfNdFljOTl6OU[0O|U>
Swiss3T3 NH7nTHFEd2yxbomt[o9zdWmwZzDBd5NigQ>? NFPBcHcyOCEEtV2= NYW4e5JrOTNiZB?= MX7JcoNz\WG|ZYOgdJJwe3SjdHWgZ4VtdCClb3zvcpku\m:{bXnu[{Bi[3Srdnn0fS=> MY[yNVQ3PDlyMh?=
HT22 MXrDfZRwfG:6aXOgRZN{[Xl? M1vXU|ExKML3TR?= M33kSFE{KGh? NVTBO|NnWHKxdHXjeJMh[WejaX7zeEBodHW2YX3heIUucW6mdXPl[EBv\XW{b37hcEBl\WG2aB?= NFjyZnAzOjhzMEizOS=>
Salivary gland stem cells NIm1TY1HfW6ldHnvckBCe3OjeR?= M4jwcFExKML3TR?= MkjWO{Bl M{LMenJm\HWlZYOgV2dUSyC|ZX7ld4NmdmOn NFTZ[nQzPThyNEW2NC=>

... Click to View More Cell Line Experimental Data

体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠,肾性高血压大鼠,及去氧皮质酮醋酸(DOCA)盐高血压大鼠,显著降低血压,这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠,持续11天,延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环,通过抑制ROCK, 降低 缺氧诱导的血管生成,和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1] [7]
+ 展开
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Formulation: 溶于DMSO,然后在盐水中稀释 (大鼠); 0.9% NaCl (小鼠)
  • Dosages: 30 mg/kg/day (大鼠);0-10 mg/kg (小鼠)
  • Administration: 口服处理 (大鼠);ip(小鼠)
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
saline
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl

CAS号 129830-38-2
稳定性 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 问题 2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

相关ROCK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID