Y-27632 2HCl

目录号:S1049

Y-27632 2HCl Chemical Structure

Molecular Weight(MW): 320.26

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

规格 价格 库存 购买数量  
RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户使用Selleck该产品发表文献59篇:

客户使用该产品的6个实验数据:

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    At day 8, cells were replated onto Matrigel-coated Transwell membranes at 106 cells/cm2 in the presence or absence of 10 mM Y-27632. TEER was measured at day 10, 2 days after replating.

    Science, 2017, 3(11):e1701679. Y-27632 2HCl purchased from Selleck.

  • YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells NFLVZVFHfW6ldHnvckBCe3OjeR?= NF3kTlIyOCEQvF2= MV6yJIg> NGnndZVFVVOR NFWy[IxKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ MnqwPVY1PzZ3NB?=
N1E-115 M3XGUGZ2dmO2aX;uJGF{e2G7 NYP0XldsOTBizszN NXLKRohvOiCq MojHSG1UVw>? NVvqOoQ5UW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? MVy5OlQ4PjV2
HeLa NXGyOnU{TnWwY4Tpc44hSXO|YYm= MlnENVAh|ryP NGjyPYI{OCCvaX6= M37rfWlvcGmkaYTzJJRp\SCob4LtZZRqd25ib3[gd5Rz\XO|IH\pZoVzeyCjbnSgeIhmKGG|c3XtZox6KG:oII\pcoN2dGmwLXPvcpRicW6rbneg[o9k[WxiYXTo[ZNqd26| MVS5OlY5ODd{
CCL39 NVnmfHF5TnWwY4Tpc44hSXO|YYm= NXfrPZRLOzBizszN NV3vXJlvOzBibXnu MV;Dc41xdGW2ZXz5JIFjd2yrc3jld{Bi[3SrdnH0bY9vKG:oIF7hMWgh\XilaHHu[4VzKE6KRUGgZpkhcW62ZXfybY5{ NIHtNWo6Pjl|M{iy
Mesothelial cells from rat mesentery NETuT4pKdn[jc3n2[UBCe3OjeR?= MlL4N|Ah|ryP MU[yNEBp MlHKRoxw[2u|IHnueoF{cX[nIHHjeIl3cXS7 NFvuNJY6QTNyOEey
NIH3T3 M1LUZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXYNVAh|ryP MXKxPEBl NF\yW3VFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NEK0dpYyODB{MUO4Oi=>
Dbl-d Mnz5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jJVlExKM7:TR?= NGTicIQyQCCm Mnv3V5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NXyyZVJuOTByMkGzPFY>
Dbl-e NGr2cVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXyUXZiOTBizszN NVHZcnRCOThiZB?= NUHX[lE2VW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= NYfrcIg1OTByMkGzPFY>
mNET1-d M37semdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvq[HYyOCEQvF2= M4rTfFE5KGR? NYDVV4pOW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? MnHKNVAxOjF|OE[=
mNET1-e M1HiSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXRNVAh|ryP Mn\rNVgh\A>? M2HURXN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp MnfaNVAxOjF|OE[=
Ras-2 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrGT2F{OTBizszN M1rRflE5KGR? MlLvV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> NFG3cooyODB{MUO4Oi=>
Ras-4 Mk\xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVmxNEDPxE1? Mn\oNVgh\A>? NWjTV49ZW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NYfVfnlrOTByMkGzPFY>
Src-1 NXj5d2MyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\keFExKM7:TR?= MonpNVgh\A>? M3HHfWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NVfI[2tLOTByMkGzPFY>
Src-4 NVzqWWpoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{D5cVExKM7:TR?= NF7SOYoyQCCm MlvsSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MVixNFAzOTN6Nh?=
NIH3T3 NInEUVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXPKbolpOTBizszN MojyNVgh\A>? MXvEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MV:xNFAzOTN6Nh?=
Src-1 MoD5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\CeVExKM7:TR?= MWWxPEBl NFzqOXdFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MknuNVAxOjF|OE[=
Src-2 Mm\hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYWxNEDPxE1? NF\HcIYyQCCm MV3Ec4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MX2xNFAzOTN6Nh?=
SW620 NED6ZWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHL5UnEyOCEQvF2= MUmxPEBl MV3Ec4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To M4qyPVExODJzM{i2
HCT15 NFzHe4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHDfVkyOCEQvF2= MXGxPEBl MYrEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MV6xNFAzOTN6Nh?=
HCT116 MlflS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn[5NVAh|ryP NWPiVG51OThiZB?= M2T0WXN1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp NH7wWoMyODB{MUO4Oi=>
LS174T MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUW5[Xd2OTBizszN NHj6VGEyQCCm NGTnUIdOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq NHPOPYQyODB{MUO4Oi=>
Neonatal rat ventricular myocytes NH;iZmhHfW6ldHnvckBCe3OjeR?= NH75bWcyOCEQvF2= MXe0PEBp MU\Jcohq[mm2czDFWE0yNWmwZIXj[YQhcW6lcnXhd4V{KGmwIIDyc5RmcW5ic4nueIhme2m|LDDj[YxtKHOrenWgZY5lKG27b3\pZpJqdGyjcjDvdodidmm8YYTpc44> NH6y[VUyODN6Nk[xNy=>
Stellate Cell M1rpUWZ2dmO2aX;uJGF{e2G7 MkjTNlUh|ryP MoTFNVUhdWmw MkLYTY5pcWKrdIOg[o9zdWG2aX;uJI9nKEZvYXP0bY4he3S{ZYPzJIZq[mW{czDhcoQheGixc4Doc5J6dGG2aX;uJI9nKG27b4PpckBtcWeqdDDjbIFqdg>? MXqxNFYxODR7Nh?=
Rat Vascular Smooth Muscle Cells M1u4XGZ2dmO2aX;uJGF{e2G7 NFjZTWkyOCEQvF2= MkH4NkBp NH\YXYpKdmirYnn0d{Bidmerb4TlcpNqdiCLST3pcoR2[2WmIHj5dIVzfHKxcHj5 M4\5XFExPjR{M{G3
PC3 MkHzSpVv[3Srb36gRZN{[Xl? Ml3ONlUh|ryP Mn3HNUBp NITPcWZKdmS3Y3XzJI1wenCqb3zv[4lk[WxiY3jhcodmew>? M2TJNVExPzJyNEex
PC3 M1H2em1q\3KjdHnvckBCe3OjeR?= NVGyeWlLOjVizszN NH;zbokyKGh? MWrJcohq[mm2czD0bIUhSk2IQj3DUUBidmRidHjlJGVITi2|dHnteYxifGWmIH3p[5JifGmxbh?= MXyxNFczODR5MR?=
PC3 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUiyOUDPxE1? NYLIboFROTdiaB?= Mkn4SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NIDvSlQyODd{MES3NS=>
LNCaP NHXp[FRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVy3XZQ1OjVizszN MXixO{Bp NHPTcHNFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MknQNVA4OjB2N{G=
Rat hepatic stellate cells NVLUdWI{TnWwY4Tpc44hSXO|YYm= M{PoT|MxKM7:TR?= NUHrdlYyPDhiaB?= NX2wZnpmTGmvaX7pd4hmeyC2aHWgdIhwe3Cqb4L5cIF1cW:wIH;mJGVzczJuIHHu[EBl\WO{ZXHz[ZMhdmW5IFTORUB{gW62aHXzbZM> M3niPFExQDR3Nk[z
Pancreatic acinar cells M4jBOGZ2dmO2aX;uJGF{e2G7 NGHKWpQyOMLizszN NGnkUZM4OCCvaX6= M175WXBwfGWwdHnheIV{KEOFSz3zeIlufWyjdHXkJJBidmO{ZXH0bYMh\W68eX3lJJNm[3KndHnvci=> MlrENVI4PDVyOEC=
C2C12 MXzGeY5kfGmxbjDBd5NigQ>? Mn[2NVDDqM7:TR?= M{HIcFYhcA>? MoC2VJJmfmWwdIOgeIhmKHOncnnu[UBxcG:|cHjvdplt[XSrb36gc4YhUVKVLUGgbY5lfWOnZDDifUBqdnO3bHnuJIFv\C:xcjDUUmYu|rF? MWKxOlI3PzF{NB?=
PC 12 NGPndmhHfW6ldHnvckBCe3OjeR?= NU\lSWJ5OTEEoN88US=> NWXYNHE6OjRiaB?= MWLBeJRmdnWjdHXzJINifGWlaH;sZY1qdmViYnnvd5lvfGinc3nz NFTFNo8yPjJzOUSyOC=>
Cynomolgus monkey embryonic stem cells M3fafWN6fG:2b4jpZ{BCe3OjeR?= MkWxNlAhyrWP MYSyOEBp NF\nWHdRem:vb4Tld{BkgUWVIHPlcIwhe3W{dnn2ZYw> MX6xPFk1ODh3NR?=
TSGH 8301 Mmi2UYloemG2aX;uJGF{e2G7 M1Hld|IxKML3TR?= Mo\VNUBp Mm\VTY5kemWjc3XzJINmdGxibXnndoF1cW:w MUOxPVg6PjR5NR?=
Swiss3T3 MkP4R49td267LX\vdo1qdmdiQYPzZZk> MlfMNVAhyrWP MnG0NVMh\A>? NWTrdJZZUW6lcnXhd4V{KHC{b4P0ZZRmKGOnbHygZ49td267LX\vdo1qdmdiYXP0bZZqfHl? M3myPFIyPDZ2OUCy
HT22 M1PzTmN6fG:2b4jpZ{BCe3OjeR?= NFLIdFcyOCEEtV2= MXexN{Bp NHHvO5VRem:2ZXP0d{Bi\2GrboP0JIdtfXSjbXH0[U1qdmS3Y3XkJI5mfXKxbnHsJIRm[XSq NFvUdY4zOjhzMEizOS=>
Salivary gland stem cells MX7GeY5kfGmxbjDBd5NigQ>? NVjGWo5nOTBiwsXN MmfjO{Bl MU\S[YR2[2W|IGPHV2Mhe2WwZYPj[Y5k\Q>? MmHINlU5ODR3NkC=

... Click to View More Cell Line Experimental Data

体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠,肾性高血压大鼠,及去氧皮质酮醋酸(DOCA)盐高血压大鼠,显著降低血压,这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠,持续11天,延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环,通过抑制ROCK, 降低 缺氧诱导的血管生成,和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1] [7]
+ 展开
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Formulation: 溶于DMSO,然后在盐水中稀释 (大鼠); 0.9% NaCl (小鼠)
  • Dosages: 30 mg/kg/day (大鼠);0-10 mg/kg (小鼠)
  • Administration: 口服处理 (大鼠);ip(小鼠)
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
saline
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl

CAS号 129830-38-2
稳定性 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 问题 2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

相关ROCK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID