Y-27632 2HCl

目录号:S1049

仅限科研使用

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

Y-27632 2HCl Chemical Structure

CAS: 129830-38-2

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
大包装 有超大折扣
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如1支10mg产品可分装为10支1mg产品

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客户使用Selleck生产的Y-27632 2HCl发表文献865篇:

产品安全说明书

ROCK抑制剂选择性比较

相关ROCK产品

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Salivary gland stem cells MX;GeY5kfGmxbjDBd5NigQ>? MnrCNVAhyrWP NYLtTHg2PyCm MoqxVoVlfWOnczDTS3NEKHOnbnXzZ4Vv[2V? MnPIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MES1OlAoRjJ3OEC0OVYxRC:jPh?=
HT22 MnLpR5l1d3SxeHnjJGF{e2G7 M3fZd|ExKML3TR?= NVv6PFBFOTNiaB?= MUjQdo91\WO2czDh[4FqdnO2IHfseZRidWG2ZT3pcoR2[2WmIH7leZJwdmGuIHTlZZRp NX\XU4pqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NVA5OzVpPkKyPFExQDN3PD;hQi=>
Swiss3T3 M13wcGNwdG:weT3mc5JucW6pIFHzd4F6 M3eyOFExKML3TR?= NHfMTYYyOyCm NXLBXIVQUW6lcnXhd4V{KHC{b4P0ZZRmKGOnbHygZ49td267LX\vdo1qdmdiYXP0bZZqfHl? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR4NEmwNkc,OjF2NkS5NFI9N2F-
TSGH 8301 MmHGUYloemG2aX;uJGF{e2G7 MWiyNEDDvU1? NEfJd3EyKGh? MXnJcoNz\WG|ZYOgZ4VtdCCvaXfyZZRqd25? Ml\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OU[0O|UoRjF7OEm2OFc2RC:jPh?=
Cynomolgus monkey embryonic stem cells M1rSSmN6fG:2b4jpZ{BCe3OjeR?= MUCyNEDDvU1? NWjUUY1{OjRiaB?= NH\UVoxRem:vb4Tld{BkgUWVIHPlcIwhe3W{dnn2ZYw> NH3kUZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEm0NFg2PSd-MUi5OFA5PTV:L3G+
PC 12 MWjGeY5kfGmxbjDBd5NigQ>? NWfITWk{OTEEoN88US=> NFi3SlkzPCCq M2XUd2F1fGWwdXH0[ZMh[2G2ZXPoc4xidWmwZTDibY9{gW62aHXzbZM> NWLRUWRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yNVk1OjRpPkG2NlE6PDJ2PD;hQi=>
C2C12 MmPDSpVv[3Srb36gRZN{[Xl? MVmxNOKh|ryP M3njVlYhcA>? M2XZTXBz\X[nboTzJJRp\SC|ZYLpcoUheGixc4Doc5J6dGG2aX;uJI9nKEmUUz2xJIlv\HWlZXSgZpkhcW6|dXzpckBidmRxb4KgWG5HNc7z NY\lT4diRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yOlcyOjRpPkG2NlY4OTJ2PD;hQi=>
Pancreatic acinar cells MnzjSpVv[3Srb36gRZN{[Xl? MojLNVDDqM7:TR?= M4XvdFcxKG2rbh?= NV;1VHQ6WG:2ZX70bYF1\XNiQ1PLMZN1cW23bHH0[YQheGGwY4LlZZRq[yCnbor5cYUhe2WlcnX0bY9v NF;MSZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMke0OVA5OCd-MUK3OFUxQDB:L3G+
Rat hepatic stellate cells Mn\zSpVv[3Srb36gRZN{[Xl? NHfHTXE{OCEQvF2= NWi0fFE5PDhiaB?= M3fqe2RqdWmwaYPo[ZMhfGinIIDoc5NxcG:{eXzheIlwdiCxZjDFdoszNCCjbnSg[IVkemWjc3XzJI5mfyCGTlGgd5lvfGinc3nz M4LydVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOES1OlY{Lz5zMEi0OVY3OzxxYU6=
LNCaP MmjXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHaNlUh|ryP MWqxO{Bp NXLhS4xmTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? NX;lcG9lRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC3NlA1PzFpPkGwO|IxPDdzPD;hQi=>
PC3 M4fTXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV[yXIdbOjVizszN MkLXNVchcA>? NF\GVIJFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NWrucHdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC3NlA1PzFpPkGwO|IxPDdzPD;hQi=>
PC3 NEDUfG9OcWe{YYTpc44hSXO|YYm= NU\tPWtsOjVizszN NIfGXmEyKGh? NIDmbG1KdmirYnn0d{B1cGViQl3GRk1EVSCjbnSgeIhmKEWJRj3zeIlufWyjdHXkJI1q\3KjdHnvci=> NFHyd4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEeyNFQ4OSd-MUC3NlA1PzF:L3G+
PC3 MYLGeY5kfGmxbjDBd5NigQ>? MXiyOUDPxE1? NUTZOXR{OSCq NGfXW5lKdmS3Y3XzJI1wenCqb3zv[4lk[WxiY3jhcodmew>? NX;qSpFzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC3NlA1PzFpPkGwO|IxPDdzPD;hQi=>
Rat Vascular Smooth Muscle Cells MmjPSpVv[3Srb36gRZN{[Xl? MX2xNEDPxE1? M4i4VVIhcA>? M2H6e2lvcGmkaYTzJIFv\2mxdHXud4lvKEmLLXnu[JVk\WRiaInw[ZJ1em:yaIm= NGPWVGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zME[0NlMyPyd-MUC2OFI{OTd:L3G+
Stellate Cell MXrGeY5kfGmxbjDBd5NigQ>? NXvpc4hnOjVizszN MV6xOUBucW5? M4ez[2lvcGmkaYTzJIZwem2jdHnvckBw\iCILXHjeIlvKHO2cnXzd{BncWKncoOgZY5lKHCqb4PwbI9zgWyjdHnvckBw\iCveX;zbY4hdGmpaISgZ4hicW5? NF;kWo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zME[wNFQ6Pid-MUC2NFA1QTZ:L3G+
Neonatal rat ventricular myocytes MUPGeY5kfGmxbjDBd5NigQ>? MX[xNEDPxE1? MmTrOFghcA>? MVfJcohq[mm2czDFWE0yNWmwZIXj[YQhcW6lcnXhd4V{KGmwIIDyc5RmcW5ic4nueIhme2m|LDDj[YxtKHOrenWgZY5lKG27b3\pZpJqdGyjcjDvdodidmm8YYTpc44> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODN6Nk[xN{c,OTB|OE[2NVM9N2F-
LS174T MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDPNVAh|ryP NFLCeHgyQCCm NFf2OHVOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq NVfiPJhmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
HCT116 NUfzUHVST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV6xNEDPxE1? MUmxPEBl NY\WfmpPW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? Mn;aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
HCT15 NVS1dYU4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXIPGwyOCEQvF2= MnXqNVgh\A>? NEf2U|BFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NFS0cmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
SW620 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkOzNVAh|ryP NVj0fG97OThiZB?= M1XpU2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NF7IcYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Src-2 MnXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3vNVAh|ryP NGHVXHoyQCCm MWXEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NEXMU|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Src-1 M2j6OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWmxNEDPxE1? NVH6RlhOOThiZB?= MkO1SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> M4fJcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
NIH3T3 NWjoNJZvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXGxNEDPxE1? NV73eXZ5OThiZB?= M3fDU2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NEXBW3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Src-4 M1LyZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rj[FExKM7:TR?= MmDSNVgh\A>? M3L4bGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MlftQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Src-1 NUDkTGZFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;uSXhpOTBizszN MoTQNVgh\A>? NVTBfGVRTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M4nGTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Ras-4 NFL4bnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV3PU5pROTBizszN NY\1SYdwOThiZB?= NIjuOFBUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? M3nZSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Ras-2 M3LxO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzHNVAh|ryP NXvxUItxOThiZB?= MlvxV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> MnHKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
mNET1-e NGPOc|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\FNVAh|ryP MnzSNVgh\A>? MnXiV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> MnfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
mNET1-d NXTNdFRzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGWwSYkyOCEQvF2= MmPtNVgh\A>? MXjTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> MmjqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Dbl-e NHfCNZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XxfFExKM7:TR?= NIfRV4MyQCCm NHnQR3BOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq NYPLS|IyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Dbl-d NIrjcFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrkT|ZJOTBizszN MWixPEBl MUHTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NWPIR3ZxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
NIH3T3 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF2zdpYyOCEQvF2= NIXxNJoyQCCm M3WyOGRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> Ml\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Mesothelial cells from rat mesentery NUDSdYZmUW64YYPpeoUhSXO|YYm= NV35SGxFOzBizszN NYT0OmJnOjBiaB?= MoLrRoxw[2u|IHnueoF{cX[nIHHjeIl3cXS7 NYjkRWRFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUmzNFg4Oid-OUmzNFg4OjxxYU6=
CCL39 MVTGeY5kfGmxbjDBd5NigQ>? NEDYfGw{OCEQvF2= NFns[Vg{OCCvaX6= MXfDc41xdGW2ZXz5JIFjd2yrc3jld{Bi[3SrdnH0bY9vKG:oIF7hMWgh\XilaHHu[4VzKE6KRUGgZpkhcW62ZXfybY5{ NFSzZ4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97NkmzN|gzLz57NkmzN|gzRC:jPh?=
HeLa MnHGSpVv[3Srb36gRZN{[Xl? MUCxNEDPxE1? NUCyb4U2OzBibXnu MWnJcohq[mm2czD0bIUh\m:{bXH0bY9vKG:oIIP0doV{eyCoaXLldpMh[W6mIITo[UBie3OnbXLsfUBw\iC4aX7jeYxqdi2lb370ZYlvcW6pIH\vZ4FtKGGmaHXzbY9vew>? M1LtRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl4NkiwO|IoRjl4NkiwO|I9N2F-
N1E-115 NVzEUpkxTnWwY4Tpc44hSXO|YYm= MX[xNEDPxE1? MnLINkBp NXT6Z3puTE2VTx?= NEW3S3hKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ NUO3fGM5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOU[0O|Y2PCd-OU[0O|Y2PDxxYU6=
Swiss 3T3 cells MXPGeY5kfGmxbjDBd5NigQ>? Mm[3NVAh|ryP MWmyJIg> NH7xNmtFVVOR MUTJcohq[mm2czD0bIUh[XO|ZX3icJkhd2ZibXnjdo91fWK3bHXzJIFv\CCrboTldo1m\GmjdHWg[olt[W2nboTzJJRwKG[xcn2g[Zh1\W6mZXSgdJJw[2W|c3Xz NWqwepJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOU[0O|Y2PCd-OU[0O|Y2PDxxYU6=
Assay
Methods Test Index PMID
Western Blot p-LIMK1(Thr508) p-LIMK2(Thr505) p-Cofilin(Ser3) CDK2 KRT7 ROCK2 E-cadherin p-MLC2(Ser19) 24704720 26555939 27399334
Transwell migration assay cell migration inhibition 27694793
Immunofluorescence Neurotoxicity Assay E-cadherin beta-catenin Phalloidin 21362567 24523903 27399334
Growth inhibition assay cell proliferation 24523903
ELISA caspase-9 30320378
体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠,肾性高血压大鼠,及去氧皮质酮醋酸(DOCA)盐高血压大鼠,显著降低血压,这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠,持续11天,延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环,通过抑制ROCK, 降低 缺氧诱导的血管生成,和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1] [7]
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Dosages: 30 mg/kg/day (大鼠);0-10 mg/kg (小鼠)
  • Administration: 口服处理 (大鼠);ip(小鼠)

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
saline

10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl

CAS号 129830-38-2
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

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操作手册

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常见问题及建议解决方法

问题 1:
Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

回答:
The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

问题 2:
Could this product be used in cell culture? Do you have any reference for this application?

回答:
Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.