Y-27632 2HCl

目录号:S1049

Y-27632 2HCl Chemical Structure

Molecular Weight(MW): 320.26

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

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RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户购买Selleck的此次产品后发表的文献53篇:

客户使用该产品的6个实验数据:

  • The ROCK inhibitors fasudil and Y27632 prevented SCP2 cell bone metastasis in nude mice (n = 10 per group). Shown are BLI images of bone metastases, IHC analyses of SMAD3 C-tail phosphorylation and PTHLH, osteoclast TRAP staining, and BLI quantitation.

    J Clin Invest, 2014, 124(4): 1646-59. Y-27632 2HCl purchased from Selleck.

    At day 8, cells were replated onto Matrigel-coated Transwell membranes at 106 cells/cm2 in the presence or absence of 10 mM Y-27632. TEER was measured at day 10, 2 days after replating.

    Science, 2017, 3(11):e1701679. Y-27632 2HCl purchased from Selleck.

  • YAP nuclear localization in fibroblasts treated with PRP-Exos was blocked by Y-27632 2HCl. Scale bar: 50 μm.

    Theranostics, 2017, 7(1):81-96. Y-27632 2HCl purchased from Selleck.

    Effect of mechanical strain on cell morphology. (A) SEM analyses indicate that strain-induced cell elongation is prevented by treatment with HA1100 and Y27632. (B) Quantification of cellular area in the indicated conditions (n = 20). (C) F-actin staining of control, strained and HA1100 or Y27632-treated cells attests that inhibition of RhoA/ROCK prevents mechanical strain-induced cell elongation. *p < 0.05 compared to control without strain (CTL).

    J Mol Cell Cardiol 2014 67, 49-59. Y-27632 2HCl purchased from Selleck.

  • The Rho GTPase-JNK pathway is required for the inhibitory effects of vandetanib on Calu-6 cells invasion. Calu-6 cells were incubated for 24 h in the presence or absence of vandetanib (1 or 2 uM), SP600125 (50 or 100 uM), and Y27632 (5 or 10 uM). The morphology of the Calu-6 cells was examined under a light microscope. Scale bar: 50 um.

    Mol Neurobiol 2015 10.1007/s12035-014-9084-z. Y-27632 2HCl purchased from Selleck.

    Dev Biol 2012 370, 33-41. Y-27632 2HCl purchased from Selleck.

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Swiss 3T3 cells MUfGeY5kfGmxbjDBd5NigQ>? M4X2bFExKM7:TR?= MYWyJIg> M4O2XGROW09? NX\BeFJyUW6qaXLpeJMhfGinIHHzd4Vu[my7IH;mJI1q[3KxdIXieYxmeyCjbnSgbY51\XKvZXTpZZRmKG[rbHHt[Y51eyC2bzDmc5JuKGW6dHXu[IVlKHC{b3Pld5Nmew>? NV;LfXcyQTZ2N{[1OC=>
N1E-115 NHjhc2RHfW6ldHnvckBCe3OjeR?= MkjPNVAh|ryP NHHabpQzKGh? NEKwTlNFVVOR M1zNeGlvcGmkaYTzJJRp\SCjc4PlcYJtgSCxZjDtbYNzd3S3YoXs[ZMh[W6mIHnueIVzdWWmaXH0[UBncWyjbXXueJMhfG9iZn;ycUBmgHSnbnTl[EBxem:lZYPz[ZM> NUXLbFlrQTZ2N{[1OC=>
HeLa MX7GeY5kfGmxbjDBd5NigQ>? MlvQNVAh|ryP MmD4N|AhdWmw M1HsOmlvcGmkaYTzJJRp\SCob4LtZZRqd25ib3[gd5Rz\XO|IH\pZoVzeyCjbnSgeIhmKGG|c3XtZox6KG:oII\pcoN2dGmwLXPvcpRicW6rbneg[o9k[WxiYXTo[ZNqd26| MWO5OlY5ODd{
CCL39 MmjwSpVv[3Srb36gRZN{[Xl? NHnGZpk{OCEQvF2= NI\XN|k{OCCvaX6= M1u2OmNwdXCuZYTlcJkh[WKxbHnzbIV{KGGldHn2ZZRqd25ib3[gUoEuUCCneHPoZY5o\XJiTljFNUBjgSCrboTl[5JqdnN? MljuPVY6OzN6Mh?=
Mesothelial cells from rat mesentery NVTQWFJ4UW64YYPpeoUhSXO|YYm= MlrPN|Ah|ryP MXqyNEBp M2nmS2Jtd2OtczDpcpZie2m4ZTDhZ5Rqfmm2eR?= Ml\CPVk{ODh5Mh?=
NIH3T3 M1LEdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjMWY1FOTBizszN M4TiNVE5KGR? NFjjc5dFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MX:xNFAzOTN6Nh?=
Dbl-d MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVmxNEDPxE1? M2G0elE5KGR? MVTTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> MX[xNFAzOTN6Nh?=
Dbl-e MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH[y[mQyOCEQvF2= M160WVE5KGR? NFLQZ|BOd2SncnH0[Yx6KGmwaHnibZR{KGOnbHyg[5Jwf3Sq NWjaZY5kOTByMkGzPFY>
mNET1-d MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWOxNEDPxE1? M3vK[lE5KGR? MVXTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NGfpTFMyODB{MUO4Oi=>
mNET1-e MmfOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmfiNVAh|ryP M{W2blE5KGR? MWLTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> MlXjNVAxOjF|OE[=
Ras-2 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfFbFA3OTBizszN MXSxPEBl MkDGV5Rzd26pbImgbY5pcWKrdIOgZ4VtdCCpcn;3eIg> M2GyS|ExODJzM{i2
Ras-4 MmjNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TpTVExKM7:TR?= MXKxPEBl MVfTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> MljXNVAxOjF|OE[=
Src-1 NH\oZ3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vVRVExKM7:TR?= MVKxPEBl MkX3SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> MWSxNFAzOTN6Nh?=
Src-4 M1Hsdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVGxNEDPxE1? Ml\NNVgh\A>? M4\XR2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MmrnNVAxOjF|OE[=
NIH3T3 NGLySHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmfuNVAh|ryP NV:0S5FUOThiZB?= NFTsTYFFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MmL5NVAxOjF|OE[=
Src-1 M{ntUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\OXFAyOCEQvF2= NVPXUopVOThiZB?= M{jifmRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NXS4fHJUOTByMkGzPFY>
Src-2 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDSNVAh|ryP MoezNVgh\A>? Mnn5SI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NX\aXIt{OTByMkGzPFY>
SW620 M1jURWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjYNVAh|ryP M4TVRVE5KGR? MXfEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NH\RV20yODB{MUO4Oi=>
HCT15 M4DyfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVS3OXp1OTBizszN NVj1eoMxOThiZB?= MUnEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NGOzTZkyODB{MUO4Oi=>
HCT116 NGjaWoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mom3NVAh|ryP MlS2NVgh\A>? NGft[4RUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? M3\nPVExODJzM{i2
LS174T M3\iUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUn5N4dMOTBizszN NUGzZWFQOThiZB?= MX;Nc4RmemG2ZXz5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp MXexNFAzOTN6Nh?=
Neonatal rat ventricular myocytes NX;DWlVzTnWwY4Tpc44hSXO|YYm= M4Dv[VExKM7:TR?= NFjDW|Y1QCCq MWjJcohq[mm2czDFWE0yNWmwZIXj[YQhcW6lcnXhd4V{KGmwIIDyc5RmcW5ic4nueIhme2m|LDDj[YxtKHOrenWgZY5lKG27b3\pZpJqdGyjcjDvdodidmm8YYTpc44> MonLNVA{QDZ4MUO=
Stellate Cell M1nnSmZ2dmO2aX;uJGF{e2G7 M4DwOlI2KM7:TR?= NFjpSIwyPSCvaX6= NYHve|lOUW6qaXLpeJMh\m:{bXH0bY9vKG:oIF[tZYN1cW5ic4Ty[ZN{KG[rYnXyd{BidmRicHjvd5Bpd3K7bHH0bY9vKG:oIH35c5NqdiCuaXfoeEBkcGGrbh?= M3[wdVExPjByNEm2
Rat Vascular Smooth Muscle Cells NYPLem8zTnWwY4Tpc44hSXO|YYm= MX[xNEDPxE1? MVuyJIg> NHTIZXJKdmirYnn0d{Bidmerb4TlcpNqdiCLST3pcoR2[2WmIHj5dIVzfHKxcHj5 MUOxNFY1OjNzNx?=
PC3 MlfxSpVv[3Srb36gRZN{[Xl? MmfDNlUh|ryP NV\rfItwOSCq MW\JcoR2[2W|IH3vdpBpd2yxZ3njZYwh[2ijbnfldy=> NHWzN|QyODd{MES3NS=>
PC3 MlrWUYloemG2aX;uJGF{e2G7 M{nKXlI2KM7:TR?= M2niZ|EhcA>? NHHKdnFKdmirYnn0d{B1cGViQl3GRk1EVSCjbnSgeIhmKEWJRj3zeIlufWyjdHXkJI1q\3KjdHnvci=> NIGxeXAyODd{MES3NS=>
PC3 Mn\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUWyOUDPxE1? MYmxO{Bp M4\OUmRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MUexNFczODR5MR?=
LNCaP M4qyR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUmyOUDPxE1? M3P2flE4KGh? NFHhfGVFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NHr4TW8yODd{MES3NS=>
Rat hepatic stellate cells MYLGeY5kfGmxbjDBd5NigQ>? NEH0fYo{OCEQvF2= M4DZZlQ5KGh? NEeweWpFcW2rbnnzbIV{KHSqZTDwbI9{eGixconsZZRqd25ib3[gSZJsOixiYX7kJIRm[3KnYYPld{Bv\XdiRF7BJJN6dnSqZYPpdy=> MWSxNFg1PTZ4Mx?=
Pancreatic acinar cells Mke2SpVv[3Srb36gRZN{[Xl? NHXwUXQyOMLizszN MmS2O|AhdWmw NGTxR5lRd3SnboTpZZRmeyCFQ1utd5RqdXWuYYTl[EBx[W6lcnXheIlkKGWweont[UB{\WO{ZYTpc44> NXO1dnNHOTJ5NEWwPFA>
C2C12 NWHWO2doTnWwY4Tpc44hSXO|YYm= M1nBUFExyqEQvF2= NUDtNXVUPiCq MY\QdoV3\W62czD0bIUhe2W{aX7lJJBpd3OyaH;yfYxifGmxbjDv[kBKWlNvMTDpcoR2[2WmIHL5JIlve3WuaX6gZY5lN2:{IGTOSk3PuQ>? NVrwToFxOTZ{NkexNlQ>
PC 12 NXiz[5ZJTnWwY4Tpc44hSXO|YYm= NWnaNFJVOTEEoN88US=> MX2yOEBp NHHzN|ZCfHSnboXheIV{KGOjdHXjbI9t[W2rbnWgZolwe3mwdHjld4l{ MWGxOlIyQTR{NB?=
Cynomolgus monkey embryonic stem cells NWHuOmRlS3m2b4TvfIlkKEG|c3H5 M13tR|IxKML3TR?= MkPyNlQhcA>? NHLCfmRRem:vb4Tld{BkgUWVIHPlcIwhe3W{dnn2ZYw> MXmxPFk1ODh3NR?=
TSGH 8301 NEf1O45OcWe{YYTpc44hSXO|YYm= NHHYSXYzOCEEtV2= NIHSc2oyKGh? M33RVmlv[3KnYYPld{Bk\WyuIH3p[5JifGmxbh?= MoTiNVk5QTZ2N{W=
Swiss3T3 M3T2NWNwdG:weT3mc5JucW6pIFHzd4F6 NHXVWmIyOCEEtV2= MmHBNVMh\A>? MXTJcoNz\WG|ZYOgdJJwe3SjdHWgZ4VtdCClb3zvcpku\m:{bXnu[{Bi[3Srdnn0fS=> MXmyNVQ3PDlyMh?=
HT22 NXXiWZRDS3m2b4TvfIlkKEG|c3H5 MoDFNVAhyrWP MmG3NVMhcA>? Mk\ZVJJwfGWldIOgZYdicW6|dDDncJV1[W2jdHWtbY5lfWOnZDDu[ZVzd26jbDDk[YF1cA>? M2WzbVIzQDFyOEO1
Salivary gland stem cells M2\JTmZ2dmO2aX;uJGF{e2G7 NHLCPZQyOCEEtV2= NIHZZlM4KGR? NUOwXGJ6WmWmdXPld{BUT1OFIIPlcoV{[2WwY3W= Mn\QNlU5ODR3NkC=

... Click to View More Cell Line Experimental Data

体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠,肾性高血压大鼠,及去氧皮质酮醋酸(DOCA)盐高血压大鼠,显著降低血压,这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠,持续11天,延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环,通过抑制ROCK, 降低 缺氧诱导的血管生成,和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[1] [7]
+ 展开
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Formulation: 溶于DMSO,然后在盐水中稀释 (大鼠); 0.9% NaCl (小鼠)
  • Dosages: 30 mg/kg/day (大鼠);0-10 mg/kg (小鼠)
  • Administration: 口服处理 (大鼠);ip(小鼠)
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
saline
10mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl

CAS号 129830-38-2
稳定性 powder
in solvent
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答:

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 问题 2:

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答:

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

相关ROCK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID