Y-27632 2HCl

For research use only. Not for use in humans.

目录号：S1049

Y-27632 2HCl Chemical Structure

CAS No. 129830-38-2

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂，无细胞试验中Ki为140 nM，比对其他激酶包括PKC，cAMP依赖性蛋白激酶，MLCK和PAK的作用强200多倍。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户使用Selleck生产的Y-27632 2HCl发表文献585篇：

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂，无细胞试验中Ki为140 nM，比对其他激酶包括PKC，cAMP依赖性蛋白激酶，MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)		 ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性，Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化，而抑制多种兴奋剂而不是KCl，包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩，IC50为0.3-1 μM。Y-27632 作用于培养的细胞，抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理，阻断 Rho调节的肌动球蛋白的激活，也阻断LPA刺激的MM1 细胞入侵活性，这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES)，显著减少分离诱导的凋亡，提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Salivary gland stem cells NFTXNlZHfW6ldHnvckBCe3OjeR?= M1\NVlExKML3TR?= NFXWWlU4KGR? M3WzSXJm\HWlZYOgV2dUSyC|ZX7ld4NmdmOn M1nzbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEC0OVYxLz5{NUiwOFU3ODxxYU6=
HT22 MonXR5l1d3SxeHnjJGF{e2G7 Ml7PNVAhyrWP M2rPblE{KGh? MXvQdo91\WO2czDh[4FqdnO2IHfseZRidWG2ZT3pcoR2[2WmIH7leZJwdmGuIHTlZZRp NEjDcXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkixNFg{PSd-MkK4NVA5OzV:L3G+
Swiss3T3 NVXhZ2V2S2:ub375MYZwem2rbnegRZN{[Xl? MUixNEDDvU1? M2DPTVE{KGR? NYDZVII5UW6lcnXhd4V{KHC{b4P0ZZRmKGOnbHygZ49td267LX\vdo1qdmdiYXP0bZZqfHl? M2LNVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNE[0PVAzLz5{MUS2OFkxOjxxYU6=
TSGH 8301 MYLNbYdz[XSrb36gRZN{[Xl? NHfjeHQzOCEEtV2= NH\3T5QyKGh? MULJcoNz\WG|ZYOgZ4VtdCCvaXfyZZRqd25? M4TPTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEm2OFc2Lz5zOUi5OlQ4PTxxYU6=
Cynomolgus monkey embryonic stem cells MXjDfZRwfG:6aXOgRZN{[Xl? Mn;oNlAhyrWP MXyyOEBp MYTQdo9ud3SnczDjfWVUKGOnbHygd5Vzfmm4YXy= Mkn3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7NEC4OVUoRjF6OUSwPFU2RC:jPh?=
PC 12 MV7GeY5kfGmxbjDBd5NigQ>? NFm4[JYyOMLizszN MY[yOEBp NIrDR5dCfHSnboXheIV{KGOjdHXjbI9t[W2rbnWgZolwe3mwdHjld4l{ M{TGVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MkG5OFI1Lz5zNkKxPVQzPDxxYU6=
C2C12 MUHGeY5kfGmxbjDBd5NigQ>? MVyxNOKh|ryP NIrFeFk3KGh? NVL2e4hWWHKndnXueJMhfGinIIPldolv\SCyaH;zdIhwenmuYYTpc44hd2ZiSWLTMVEhcW6mdXPl[EBjgSCrboP1cIlvKGGwZD;vdkBVVkZvzsG= NX;nemtFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yOlcyOjRpPkG2NlY4OTJ2PD;hQi=>
Pancreatic acinar cells MlT0SpVv[3Srb36gRZN{[Xl? MUWxNOKh|ryP MoLTO|AhdWmw MWfQc5RmdnSrYYTld{BES0tvc4TpcZVt[XSnZDDwZY5kemWjdHnjJIVvgnmvZTDz[YNz\XSrb36= M{nLUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{N{S1NFgxLz5zMke0OVA5ODxxYU6=
Rat hepatic stellate cells Mme2SpVv[3Srb36gRZN{[Xl? NF3wfng{OCEQvF2= NV3D[VJUPDhiaB?= M2W0fmRqdWmwaYPo[ZMhfGinIIDoc5NxcG:{eXzheIlwdiCxZjDFdoszNCCjbnSg[IVkemWjc3XzJI5mfyCGTlGgd5lvfGinc3nz M3G4RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOES1OlY{Lz5zMEi0OVY3OzxxYU6=
LNCaP NV3LXJJ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LtZlI2KM7:TR?= Ml\INVchcA>? MYrEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MojrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB5MkC0O|EoRjFyN{KwOFcyRC:jPh?=
PC3 MmXxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFjGfo0zPSEQvF2= MmHvNVchcA>? NHja[29Fd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NUOzdHR7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC3NlA1PzFpPkGwO|IxPDdzPD;hQi=>
PC3 MlW1UYloemG2aX;uJGF{e2G7 MV6yOUDPxE1? NGfLXlIyKGh? M1vNUWlvcGmkaYTzJJRp\SCETV\CMWNOKGGwZDD0bIUhTUeILYP0bY12dGG2ZXSgcYloemG2aX;u Mn;5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB5MkC0O|EoRjFyN{KwOFcyRC:jPh?=
PC3 MnywSpVv[3Srb36gRZN{[Xl? MVOyOUDPxE1? NVXjcWIyOSCq NVPYfZI3UW6mdXPld{Bud3KyaH;sc4dq[2GuIHPoZY5o\XN? M33HZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyN{KwOFcyLz5zMEeyNFQ4OTxxYU6=
Rat Vascular Smooth Muscle Cells M3vUfGZ2dmO2aX;uJGF{e2G7 M4PuOlExKM7:TR?= M1nqdFIhcA>? NHnFUZlKdmirYnn0d{Bidmerb4TlcpNqdiCLST3pcoR2[2WmIHj5dIVzfHKxcHj5 M3[4[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNkSyN|E4Lz5zME[0NlMyPzxxYU6=
Stellate Cell NF2zTXVHfW6ldHnvckBCe3OjeR?= M2rkdlI2KM7:TR?= M4XRNVE2KG2rbh?= M{\GfGlvcGmkaYTzJIZwem2jdHnvckBw\iCILXHjeIlvKHO2cnXzd{BncWKncoOgZY5lKHCqb4PwbI9zgWyjdHnvckBw\iCveX;zbY4hdGmpaISgZ4hicW5? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODZyMES5Okc,OTB4MEC0PVY9N2F-
Neonatal rat ventricular myocytes NIjsWJRHfW6ldHnvckBCe3OjeR?= MXGxNEDPxE1? NXnB[oQ5PDhiaB?= M3W1N2lvcGmkaYTzJGVVNTFvaX7keYNm\CCrbnPy[YF{\XNiaX6gdJJwfGWrbjDzfY51cGW|aYOsJINmdGxic3n6[UBidmRibYnv[oljemmubHHyJI9z\2GwaYrheIlwdg>? NX3hSGp1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCzPFY3OTNpPkGwN|g3PjF|PD;hQi=>
LS174T MofWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TmWVExKM7:TR?= NX\Ub5ExOThiZB?= Mnj2UY9l\XKjdHXsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? M1\XOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
HCT116 M{\aW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fNTVExKM7:TR?= M3XIUlE5KGR? NGS3OodUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? M1SwZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
HCT15 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLMNVAh|ryP NIr4Xm0yQCCm Mm\KSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NHX4[o09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
SW620 NWH1WWNDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlraNVAh|ryP NGHXc4wyQCCm M3P2fWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Src-2 NEntUplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXSxNEDPxE1? MWSxPEBl NWPW[Hk2TG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? NUW2TnlCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Src-1 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV:xNEDPxE1? MnnVNVgh\A>? MkDuSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NHzNZYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
NIH3T3 M2G2PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXyPHMyOCEQvF2= M4LiRVE5KGR? NXzwe4I4TG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Src-4 Mk\QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWexNEDPxE1? MWKxPEBl NEXLUmpFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Src-1 M2jJOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;xNVAh|ryP MWKxPEBl NHTON|hFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NYXHU5RVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Ras-4 NEPse5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mli3NVAh|ryP M2\hcVE5KGR? MVfTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> Mlf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Ras-2 NYfzelMyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LnblExKM7:TR?= NGHwOJQyQCCm NWrofWJjW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
mNET1-e M3\qRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HEflExKM7:TR?= NUXBN|JlOThiZB?= MXvTeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
mNET1-d MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVe2bWN5OTBizszN NFrKbYsyQCCm NInERllUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Dbl-e MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUf0TIt6OTBizszN NWDp[2JTOThiZB?= MnvkUY9l\XKjdHXsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? M1TjZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
Dbl-d M4DXTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\4fWYyOCEQvF2= NUjGZWtGOThiZB?= NV7tfoxUW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? M4rDcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
NIH3T3 NWP6Z4piT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFvicXkyOCEQvF2= NFHuNpMyQCCm NGKxNXVFd2W|IH7veEBqdmirYnn0JINmdGxiZ4Lve5Rp NWXkN5lFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
Mesothelial cells from rat mesentery M1KzT2lvfmG|aY\lJGF{e2G7 MXezNEDPxE1? MonGNlAhcA>? MmTyRoxw[2u|IHnueoF{cX[nIHHjeIl3cXS7 MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QTNyOEeyK|46QTNyOEeyQE9iRg>?
CCL39 MomwSpVv[3Srb36gRZN{[Xl? NYfzNItOOzBizszN NWnON2RMOzBibXnu NUXKVm57S2:vcHzleIVtgSCjYn;sbZNp\XNiYXP0bZZifGmxbjDv[kBP[S2KIHX4Z4hidmencjDOTGUyKGK7IHnueIVoemmwcx?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86Pjl|M{iyK|46Pjl|M{iyQE9iRg>?
HeLa MWHGeY5kfGmxbjDBd5NigQ>? NF7kOY0yOCEQvF2= MmPaN|AhdWmw NXvybZBPUW6qaXLpeJMhfGinIH\vdo1ifGmxbjDv[kB{fHKnc4Og[olj\XK|IHHu[EB1cGViYYPz[Y1jdHlib3[geolv[3WuaX6tZ49vfGGrbnnu[{Bnd2OjbDDh[Ihme2mxboO= M{TXUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl4NkiwO|IoRjl4NkiwO|I9N2F-
N1E-115 M{Pn[GZ2dmO2aX;uJGF{e2G7 NGrDSm0yOCEQvF2= M2jYXVIhcA>? NV23U5VLTE2VTx?= Mm\GTY5pcWKrdIOgeIhmKGG|c3XtZox6KG:oIH3pZ5JwfHWkdXzld{BidmRiaX70[ZJu\WSrYYTlJIZqdGGvZX70d{B1dyCob4LtJIV5fGWwZHXkJJBzd2Onc4Pldy=> M2HUXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl4NEe2OVQoRjl4NEe2OVQ9N2F-
Swiss 3T3 cells NFrBe3pHfW6ldHnvckBCe3OjeR?= M4HBN|ExKM7:TR?= MV2yJIg> MlnBSG1UVw>? NGSxcJdKdmirYnn0d{B1cGViYYPz[Y1jdHlib3[gcYlkem:2dXL1cIV{KGGwZDDpcpRmem2nZHnheIUh\mmuYX3lcpR{KHSxIH\vdo0h\Xi2ZX7k[YQheHKxY3Xzd4V{ M3PIOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl4NEe2OVQoRjl4NEe2OVQ9N2F-

... Click to View More Cell Line Experimental Data
Assay
Methods Test Index PMID
Western Blot
p-LIMK1(Thr508); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

p-LIMK2(Thr505); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

p-Cofilin(Ser3); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

CDK2; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

KRT7; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

ROCK2; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

E-cadherin; 

PubMed: 27399334     


The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets in SW-480-FOXM1D cells treated with Y-27632.

p-MLC2(Ser19); 

PubMed: 27399334     


The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets in SW-480-FOXM1D cells treated with Y-27632.

24704720 26555939 27399334
Transwell migration assay
cell migration inhibition ; 

PubMed: 27694793     


Data represent mean ± SEM, n=3. Compared with control, * P<0.05, ** P<0.01. Compared with control, Y-27632, or EGCG, *** P<0.01.

27694793
Immunofluorescence
Neurotoxicity Assay; 

PubMed: 21362567     


Representations of neurodegeneration in H9-, HUF5- and G2019S-iPSC derived neurons when treated with neurotoxins, H2O2 and MG-132, and ROCK inhibitor Y-27632. Scale bar = 50 µm.

E-cadherin; 

PubMed: 24523903     


The localized patterns of E-cadherin and b-catenin were examined using specific antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24 hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity. 

beta-catenin; 

PubMed: 24523903     


The localized patterns of E-cadherin and b-catenin were examined using specific antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24 hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity. 

Phalloidin; 

PubMed: 27399334     


Triple IF staining for F-actin (phalloidin, green) E-cadherin (b) (red) and nuclei (DAPI, blue) showed decreased F-actin, increased E-cadherin and altered cell shape and size in SW-480-FOXM1D cells treated with the ROCKs inhibitor Y-27632 or fasudil (both 10 μM, for 24 h). Scale bar, 25 μm. 

21362567 24523903 27399334
Growth inhibition assay
cell proliferation ; 

PubMed: 24523903     


MCF-7 cells and MDA-MB-231 cells were cultured on tissue culture plates for four days with or without Y-27632 (20 µM). Relative cell proliferation rates were determined by the cell number assayed using CCK-8 kit. The data are expressed as the mean ± SD. n = 3 culture dishes. The p-value was less than 0.05 for comparisons between control (Control) and treatment groups (Y-27632) in MCF-7 cells. *, p<0.05.

24523903
ELISA
caspase-9; 

PubMed: 30320378     


Caspase-9 activity was measured via ELISA. Mst1-mediated casapse-9 activation was inhibited by Y-27632 in A549 cells. *P<0.05. Ad, adenovirus; Mst1, mammalian STE20-like kinase 1.

30320378
体内研究 Y-27632按30 mg/kg剂量口服处理自发性高血压大鼠，肾性高血压大鼠，及去氧皮质酮醋酸(DOCA)盐高血压大鼠，显著降低血压，这种作用存在剂量依赖性。[1]Y-27632按每小时0.55 μL通过植入泵持续处理表达Val14-RhoA的大鼠，持续11天，延迟 MM1 细胞入侵。[2] Y-27632作用于肺循环，通过抑制ROCK, 降低 缺氧诱导的血管生成，和血管重构。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验：[1] [7]
- 合并
  • Animal Models: 携带自发或诱发高血压的雄性Wistar大鼠; 艾利希腹水癌小鼠模型
  • Dosages: 30 mg/kg/day (大鼠)；0-10 mg/kg （小鼠）
  • Administration: 口服处理 (大鼠)；ip（小鼠）
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 64 mg/mL warmed (199.83 mM)
Water 14 mg/mL (43.71 mM)
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)：
saline
 10mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.26
化学式

C14H21N3O.2HCl
CAS号 129830-38-2
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量 只
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量：

总分子量：g/mol

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1：

    Is there any data about the Amax (maximum attraction luminosity) and extinction coefficient of this compound?

  • 回答：

    The wavelength we used to test HPLC is 260nm while the extinction coefficient is unknown.

  • 问题 2：

    Could this product be used in cell culture? Do you have any reference for this application?

  • 回答：

    Yes. The Y-27632 can be used in cell culture certainly. Here is the reference website: http://molpharm.aspetjournals.org/content/57/5/976.full.

Selleck产品目录册

ROCK Signaling Pathway Map

ROCK Inhibitors with Unique Features

  • 选择性强的Rock抑制剂

    Fasudil (HA-1077) HCl : ROCK-II-selective, Ki=0.33 μM.

  • 活性最高的ROCK抑制剂

    GSK429286A : ROCK1, IC50=14 nM; ROCK2, IC50=63 nM.

  • 用于临床的ROCK抑制剂

    Fasudil (HA-1077) HCl : Phase III for scleroderma.

  • 最新的ROCK抑制剂

    RKI-1447 : Highly potent inhibitor of ROCK1 and ROCK2, with IC50 of 14.5 nM and 6.2 nM, respectively.

相关ROCK产品

  • Ro-3306 New

    RO-3306是一种ATP竞争性的选择性 CDK1 抑制剂，Ki 为20 nM，选择性是其他各种人类激酶的15倍多。RO-3306可增强p53介导的 Bax 激活和线粒体的凋亡。

  • CCG-1423 New

    CCG-1423是一种特定的 RhoA 通路抑制剂，能够抑制SRF介导的转录。

  • ML141 New

    ML141 (CID-2950007) 是 Rho 家族GTPase cdc42的一种有效的，选择性可逆非竞争性抑制剂，IC50为200 nM。对cdc42比对其他Rho家族中的GTPases(如Rac1, Rab2, Rab7)更有选择性。 ML141 与 p38 激活增加有关，并可能诱导p38依赖性凋亡/衰老。ML141还可保护神经母细胞瘤细胞免受二甲双胍(metformin)诱导的凋亡。

  • Thiazovivin

    Thiazovivin是一种新型ROCK抑制剂，无细胞试验中IC50为0.5 μM，在单细胞分离后，促进人胚胎干细胞（hESC）的存活。

  • GSK429286A

    GSK429286A (RHO-15)是一种选择性的ROCK1和ROCK2抑制剂，IC50分别为14 nM和63 nM。

    Features:More selective than Y-27632.

  • Fasudil (HA-1077) HCl

    Fasudil(HA-1077)是一种有效的、具有选择性的Rho抑制剂（ROCK2, Ki=0.33 μM），同时对PKA、PKG、PKC和MLCK也具有抑制活性，Ki值分别为1.6 μM、1.6 μM、3.3 μM、36 μM。Fasudil 也是calcium channel的阻滞剂。

  • RKI-1447

    RKI-1447是一种有效的ROCK1和ROCK2抑制剂，IC50分别为14.5 nM和6.2 nM，有抗侵入和抗肿瘤活性。

  • Palbociclib (PD-0332991) HCl

    Palbociclib (PD-0332991) HCl是一种高度选择性的CDK4/6抑制剂，无细胞试验中IC50分别为11 nM/16 nM。它对CDK1/2/5，EGFR，FGFR，PDGFR，InsR等没有抑制活性。Phase 3。

    Features:PD-0332991作为有应用前景的工具测定CDK激酶是否具有明显的治疗价值。

  • Alisertib (MLN8237)

    Alisertib (MLN8237)是一种选择性Aurora A抑制剂，无细胞试验中IC50为1.2 nM，作用于Aurora A比作用于Aurora B选择性强200倍以上。Alisertib 可诱导细胞周期阻滞、细胞凋亡与自噬。Phase 3。

    Features:MLN8237 是第一个口服有效的小分子Aurora A激酶选择性抑制剂。

  • Adavosertib (MK-1775)

    Adavosertib (MK-1775, AZD1775)是一种有效的，高选择性Wee1抑制剂，无细胞试验中IC50为5.2 nM；阻碍G2期DNA损伤检验点。Phase 2。

    Features:First reported Wee1 inhibitor

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID