Y-27632 2HCl

For research use only. Not for use in humans.

目录号:S1049

Y-27632 2HCl Chemical Structure

CAS No. 129830-38-2

Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1278.83 现货
RMB 576.16 现货
RMB 972.96 现货
RMB 1396.58 现货
RMB 4674.45 现货
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客户使用Selleck生产的Y-27632 2HCl发表文献649篇:

产品安全说明书

ROCK抑制剂选择性比较

生物活性

产品描述 Y-27632 2HCl是一种选择性ROCK1(p160ROCK)抑制剂,无细胞试验中Ki为140 nM,比对其他激酶包括PKC,cAMP依赖性蛋白激酶,MLCK和PAK的作用强200多倍。
靶点
ROCK1 (p160ROCK) [1]
(Cell-free assay)
ROCK2 [6]
(Cell-free assay)
140 nM(Ki) 300 nM(Ki)
体外研究

Y-27632也同等有效抑制ROCK-II。Y-27632作用于PKC, cAMP依赖的蛋白激酶和 肌球蛋白轻链激酶(MLCK)几乎没有活性,Ki分别为26 μM, 25 μM, 和 > 250 μM。Y-27632通过选择性抑制Ca2+敏感化,而抑制多种兴奋剂而不是KCl,包括Phenylephrine, Histamine, Acetylcholine, Serotonin, Endothelin,和Thromboxane诱导的平滑肌收缩,IC50为0.3-1 μM。Y-27632 作用于培养的细胞,抑制Rho诱导的, p160ROCK调节的应力纤维的形成。[1] Y-27632处理,阻断 Rho调节的肌动球蛋白的激活,也阻断LPA刺激的MM1 细胞入侵活性,这种作用存在浓度依赖性。[2] 10 μM Y-27632 处理在无血清悬浮(SFEB)培养基中的人类胚胎干细胞(hES),显著减少分离诱导的凋亡,提高克隆效率(从~1%提高到~27%), 转基因后促进亚克隆,且使SFEB培养的hES细胞存活及分化成Bf1+皮质和基底端脑祖细胞。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Salivary gland stem cells MkXUSpVv[3Srb36gRZN{[Xl? MmfCNVAhyrWP NHnafGw4KGR? MVfS[YR2[2W|IGPHV2Mhe2WwZYPj[Y5k\Q>? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPThyNEW2NEc,OjV6MES1OlA9N2F-
HT22 NIfYellEgXSxdH;4bYMhSXO|YYm= M2S4eFExKML3TR?= NXu5[mVKOTNiaB?= NHH3b2dRem:2ZXP0d{Bi\2GrboP0JIdtfXSjbXH0[U1qdmS3Y3XkJI5mfXKxbnHsJIRm[XSq MkfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MUC4N|UoRjJ{OEGwPFM2RC:jPh?=
Swiss3T3 NF;Cd2pEd2yxbomt[o9zdWmwZzDBd5NigQ>? M4HQOlExKML3TR?= MoG0NVMh\A>? M{nUZmlv[3KnYYPld{Bxem:|dHH0[UBk\WyuIHPvcI9vgS2ob4LtbY5oKGGldHn2bZR6 MmjIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF2NkS5NFIoRjJzNE[0PVAzRC:jPh?=
TSGH 8301 MULNbYdz[XSrb36gRZN{[Xl? NHSze3QzOCEEtV2= NXTPOnp6OSCq NH;SRVNKdmO{ZXHz[ZMh[2WubDDtbYdz[XSrb36= M2LudlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OEm2OFc2Lz5zOUi5OlQ4PTxxYU6=
Cynomolgus monkey embryonic stem cells M4DXPWN6fG:2b4jpZ{BCe3OjeR?= MlHINlAhyrWP NEj5[ZczPCCq MlvEVJJwdW:2ZYOgZ5lGWyClZXzsJJN2en[rdnHs MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDl2MEi1OUc,OTh7NEC4OVU9N2F-
PC 12 M1PYNWZ2dmO2aX;uJGF{e2G7 NX3JUpJUOTEEoN88US=> MkLSNlQhcA>? NYP5dW1DSXS2ZX71ZZRmeyClYYTlZ4hwdGGvaX7lJIJqd3O7boTo[ZNqew>? M2fSelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MkG5OFI1Lz5zNkKxPVQzPDxxYU6=
C2C12 NGjDe|ZHfW6ldHnvckBCe3OjeR?= Mn;tNVDDqM7:TR?= MnG4OkBp NH7QcVFRemW4ZX70d{B1cGVic3XybY5mKHCqb4PwbI9zgWyjdHnvckBw\iCLUmOtNUBqdmS3Y3XkJIJ6KGmwc4XsbY4h[W6mL3;yJHRPTi4QsR?= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJ4N{GyOEc,OTZ{NkexNlQ9N2F-
Pancreatic acinar cells M{\mZ2Z2dmO2aX;uJGF{e2G7 MorpNVDDqM7:TR?= M{LJflcxKG2rbh?= MYrQc5RmdnSrYYTld{BES0tvc4TpcZVt[XSnZDDwZY5kemWjdHnjJIVvgnmvZTDz[YNz\XSrb36= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjd2NUC4NEc,OTJ5NEWwPFA9N2F-
Rat hepatic stellate cells NILpcmNHfW6ldHnvckBCe3OjeR?= MmC3N|Ah|ryP MY[0PEBp MnfqSIlucW6rc3jld{B1cGVicHjvd5Bpd3K7bHH0bY9vKG:oIFXyb|ItKGGwZDDk[YNz\WG|ZYOgcoV4KESQQTDzfY51cGW|aYO= Mk\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB6NEW2OlMoRjFyOES1OlY{RC:jPh?=
LNCaP NEjhOZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PxeVI2KM7:TR?= MkjFNVchcA>? NUjZcIVwTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? M4XZclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyN{KwOFcyLz5zMEeyNFQ4OTxxYU6=
PC3 NFXYPIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWr4b2dVOjVizszN MkT0NVchcA>? MXTEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To NFLFPGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEeyNFQ4OSd-MUC3NlA1PzF:L3G+
PC3 M4q1SG1q\3KjdHnvckBCe3OjeR?= MkLhNlUh|ryP MnzxNUBp MnvoTY5pcWKrdIOgeIhmKEKPRlKtR20h[W6mIITo[UBGT0Zvc4TpcZVt[XSnZDDtbYdz[XSrb36= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODd{MES3NUc,OTB5MkC0O|E9N2F-
PC3 NW\rbGJUTnWwY4Tpc44hSXO|YYm= Mnm4NlUh|ryP MlnmNUBp MWPJcoR2[2W|IH3vdpBpd2yxZ3njZYwh[2ijbnfldy=> NVPOfo5WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC3NlA1PzFpPkGwO|IxPDdzPD;hQi=>
Rat Vascular Smooth Muscle Cells NYfo[VZsTnWwY4Tpc44hSXO|YYm= NWjTb5dQOTBizszN NIXYVI8zKGh? NYjZd|JMUW6qaXLpeJMh[W6paX;0[Y5{cW5iSVmtbY5lfWOnZDDofZBmenS{b4DofS=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODZ2MkOxO{c,OTB4NEKzNVc9N2F-
Stellate Cell NVzQR3FnTnWwY4Tpc44hSXO|YYm= Mlj2NlUh|ryP NXe2eXFpOTVibXnu MoL1TY5pcWKrdIOg[o9zdWG2aX;uJI9nKEZvYXP0bY4he3S{ZYPzJIZq[mW{czDhcoQheGixc4Doc5J6dGG2aX;uJI9nKG27b4PpckBtcWeqdDDjbIFqdg>? M3L3VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyNkCwOFk3Lz5zME[wNFQ6PjxxYU6=
Neonatal rat ventricular myocytes MWDGeY5kfGmxbjDBd5NigQ>? NXjRfHVjOTBizszN MofROFghcA>? NXHIUIpsUW6qaXLpeJMhTVRvMT3pcoR2[2WmIHnuZ5Jm[XOnczDpckBxem:2ZXnuJJN6dnSqZYPpd{wh[2WubDDzbZpmKGGwZDDtfY9ncWK{aXzsZZIhd3KpYX7pfoF1cW:w M4m3eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyM{i2OlE{Lz5zMEO4OlYyOzxxYU6=
LS174T MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3nQelExKM7:TR?= MVWxPEBl NVu4PYE{VW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= MoHrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
HCT116 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUDsPWFPOTBizszN MYexPEBl NHjCd|BUfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? MnvXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
HCT15 NVrNNFdIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYGxNEDPxE1? MYmxPEBl MXnEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
SW620 Mn73S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fnWFExKM7:TR?= M2fublE5KGR? MlzuSI9meyCwb4SgbY5pcWKrdDDj[YxtKGe{b4f0bC=> NIXzeW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Src-2 M2rEb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX2yVJpsOTBizszN Mn[2NVgh\A>? MUXEc4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MkTwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Src-1 MljkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV:xNEDPxE1? M2TkXlE5KGR? M1S3b2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> M165RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyMEKxN|g3Lz5zMECyNVM5PjxxYU6=
NIH3T3 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TB[|ExKM7:TR?= MlvyNVgh\A>? MW\Ec4V{KG6xdDDpcohq[mm2IHPlcIwh\3Kxd4To MoXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Src-4 NUP1V4lMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1OzfVExKM7:TR?= Mn7lNVgh\A>? M17CU2Rw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> NFHK[pA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Src-1 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEn5ZYQyOCEQvF2= NHjYSpYyQCCm NUKzfZdWTG:nczDuc5QhcW6qaXLpeEBk\WyuIHfyc5d1cA>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Ras-4 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnvaNVAh|ryP MXyxPEBl NVTQZ5FVW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NGr2eVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Ras-2 M1zYNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnuNVAh|ryP NVjOUmFFOThiZB?= NX3O[GcxW3S{b37ncJkhcW6qaXLpeJMh[2WubDDndo94fGh? NV:wdGtORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwNlE{QDZpPkGwNFIyOzh4PD;hQi=>
mNET1-e MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUKxNEDPxE1? NW\GRmY3OThiZB?= NEO0VI9UfHKxbnfsfUBqdmirYnn0d{Bk\WyuIHfyc5d1cA>? NIrFbG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
mNET1-d MmTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYmxNEDPxE1? MkfJNVgh\A>? MW\TeJJwdmeueTDpcohq[mm2czDj[YxtKGe{b4f0bC=> NH3MemY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMECyNVM5Pid-MUCwNlE{QDZ:L3G+
Dbl-e MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn:0NVAh|ryP NWfJOnEyOThiZB?= NWfNV|JDVW:mZYLheIVtgSCrbnjpZol1eyClZXzsJIdzd3e2aB?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
Dbl-d MoTxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrmd5l2OTBizszN NI\5SFUyQCCm M1K0T3N1em:wZ3z5JIlvcGmkaYTzJINmdGxiZ4Lve5Rp MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB{MUO4Okc,OTByMkGzPFY9N2F-
NIH3T3 NGXkRZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEm5dVIyOCEQvF2= MVqxPEBl M3f1WWRw\XNibn;0JIlvcGmkaYSgZ4VtdCCpcn;3eIg> MmLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByMkGzPFYoRjFyMEKxN|g3RC:jPh?=
Mesothelial cells from rat mesentery MnflTY53[XOrdnWgRZN{[Xl? NFvZS2Q{OCEQvF2= NGTwUmEzOCCq NUDOcYs1SmyxY3vzJIlvfmG|aY\lJIFkfGm4aYT5 M4LIO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl7M{C4O|IoRjl7M{C4O|I9N2F-
CCL39 M13iVWZ2dmO2aX;uJGF{e2G7 M4j6elMxKM7:TR?= NFnZVnQ{OCCvaX6= NG\EVpREd22ybHX0[Yx6KGGkb3zpd4hmeyCjY4TpeoF1cW:wIH;mJG5iNUhiZYjjbIFv\2W{IF7ISVEh[nliaX70[YdzcW6| M2XOU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493Nzl4OUOzPFIoRjl4OUOzPFI9N2F-
HeLa MXvGeY5kfGmxbjDBd5NigQ>? MofpNVAh|ryP MX[zNEBucW5? MoHyTY5pcWKrdIOgeIhmKG[xcn3heIlwdiCxZjDzeJJme3NiZnni[ZJ{KGGwZDD0bIUh[XO|ZX3icJkhd2ZidnnuZ5VtcW5vY3;ueIFqdmmwZzDmc4NidCCjZHjld4lwdnN? MkS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwQTZ4OEC3Nkc,QTZ4OEC3Nlww[T5?
N1E-115 M3HKRmZ2dmO2aX;uJGF{e2G7 NFTCR3gyOCEQvF2= M{HYPFIhcA>? NXzGTWt6TE2VTx?= MnjHTY5pcWKrdIOgeIhmKGG|c3XtZox6KG:oIH3pZ5JwfHWkdXzld{BidmRiaX70[ZJu\WSrYYTlJIZqdGGvZX70d{B1dyCob4LtJIV5fGWwZHXkJJBzd2Onc4Pldy=> NIHx[ok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97NkS3OlU1Lz57NkS3OlU1RC:jPh?=
Swiss 3T3 cells Mo[xSpVv[3Srb36gRZN{[Xl? NVW0ZYk{OTBizszN NGntS3QzKGh? MVrEUXNQ MWPJcohq[mm2czD0bIUh[XO|ZX3icJkhd2ZibXnjdo91fWK3bHXzJIFv\CCrboTldo1m\GmjdHWg[olt[W2nboTzJJRwKG[xcn2g[Zh1\W6mZXSgdJJw[2W|c3Xz MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86PjR5NkW0K|46PjR5NkW0QE9iRg>?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western Blot
p-LIMK1(Thr508); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

p-LIMK2(Thr505); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

p-Cofilin(Ser3); 

PubMed: 24704720     


Immunoblot analysis of HCT116 and HCT116 SMAD4-/- cells. Cells were transfected transiently with either BMPR2 or pcDNA vector. Subsequently, cells were treated with 2.5 μmol/L of ROCK inhibitor (Y-27632) or PBS. Blots were incubated with antibodies against pLIMK1/2 and p-cofilin. Actin was used as a loading control.

CDK2; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

KRT7; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

ROCK2; 

PubMed: 26555939     


Western blot detected proteins changes pTR cells cultured with Y-27632. CDX2 was remarkably increased in Y-27632 treated pIVFTR-7 and pPATR-5 cells. pTR cells cultured without Y-27632 in the same condition are used as control. β-ACTIN serves as an endogenous control. 

E-cadherin; 

PubMed: 27399334     


The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets in SW-480-FOXM1D cells treated with Y-27632.

p-MLC2(Ser19); 

PubMed: 27399334     


The expression and/or phosphorylation of E-cadherin and ROCKs downstream targets in SW-480-FOXM1D cells treated with Y-27632.

24704720 26555939 27399334
Transwell migration assay
cell migration inhibition ; 

PubMed: 27694793     


Data represent mean ± SEM, n=3. Compared with control, * P<0.05, ** P<0.01. Compared with control, Y-27632, or EGCG, *** P<0.01.

27694793
Immunofluorescence
Neurotoxicity Assay; 

PubMed: 21362567     


Representations of neurodegeneration in H9-, HUF5- and G2019S-iPSC derived neurons when treated with neurotoxins, H2O2 and MG-132, and ROCK inhibitor Y-27632. Scale bar = 50 µm.

E-cadherin; 

PubMed: 24523903     


The localized patterns of E-cadherin and b-catenin were examined using specific antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24 hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity. 

beta-catenin; 

PubMed: 24523903     


The localized patterns of E-cadherin and b-catenin were examined using specific antibodies through confocal laser microscopy. MCF-7 cells were cultured for 24 hrs in the absence or presence of Y-27632 (20 µM) to inhibit ROCK activity. 

Phalloidin; 

PubMed: 27399334     


Triple IF staining for F-actin (phalloidin, green) E-cadherin (b) (red) and nuclei (DAPI, blue) showed decreased F-actin, increased E-cadherin and altered cell shape and size in SW-480-FOXM1D cells treated with the ROCKs inhibitor Y-27632 or fasudil (both 10 μM, for 24 h). Scale bar, 25 μm. 

21362567 24523903 27399334
Growth inhibition assay
cell proliferation ; 

PubMed: 24523903     


MCF-7 cells and MDA-MB-231 cells were cultured on tissue culture plates for four days with or without Y-27632 (20 µM). Relative cell proliferation rates were determined by the cell number assayed using CCK-8 kit. The data are expressed as the mean ± SD. n = 3 culture dishes. The p-value was less than 0.05 for comparisons between control (Control) and treatment groups (Y-27632) in MCF-7 cells. *, p<0.05.

24523903
ELISA