BX-795

For research use only. Not for use in humans.

目录号:S1274

BX-795 Chemical Structure

CAS No. 702675-74-9

BX-795 是一种有效的,特异性的PDK1抑制剂,在无细胞试验中IC50为6 nM,作用于PDK1比作用于PKA和PKC选择性分别高140和1600倍。同时,相比于 GSK3β,作用于PDK1的选择性高出100多倍。BX-795 可通过抑制 ULK1 来调节自噬。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1628.91 现货
RMB 1236.66 现货
RMB 2623.21 现货
RMB 4662.25 现货
RMB 7931.08 现货
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客户使用Selleck生产的BX-795发表文献43篇:

产品安全说明书

PDK抑制剂选择性比较

生物活性

产品描述 BX-795 是一种有效的,特异性的PDK1抑制剂,在无细胞试验中IC50为6 nM,作用于PDK1比作用于PKA和PKC选择性分别高140和1600倍。同时,相比于 GSK3β,作用于PDK1的选择性高出100多倍。BX-795 可通过抑制 ULK1 来调节自噬。
靶点
PDK-1 [1]
(Cell-free assay)
c-Kit [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
Chk1 [1]
(Cell-free assay)
6 nM 320 nM 430 nM 510 nM
体外研究

BX795也抑制MARK1,MARK2,MARK4,NUAK1,VEGFR,MLK1,MLK2,和MLK3,IC50分别为55,53,19,5,157,50,46和42 nM。1 μM BX795不抑制如下酪氨酸蛋白激酶: 肝配蛋白受体A2和B3,Syk,Bruton's酪氨酸激酶,和FGFR1。然而,BX795抑制VEGFR,抑制效果比TBK1低。BX795抑制TBK1-催化的IRF3在Ser396位点磷酸化随着ATP浓度上升而下降,说明BX795是ATP竞争性抑制剂。BX795阻断TBK1和IKKε调节的IRF3激活,且抑制IFN-β的产量。聚(I:C)处理后,IRF3在核中积累,但是可被BX795抑制。 BX795抑制IRF3依赖的基因转录。BX795抑制IFN-β从巨噬细胞中分泌。BX795对LPS刺激的p70核糖体S6激酶1在Thr229位点磷酸化没有效果, Thr229位点是PDK1作用靶点。BX795不影响IKKα/β复合体或LPS,聚(I:C),IL-1α,或TNFα促进的NFκB依赖的基因转录的激活。BX795作用于IL-1α或TNFα刺激的MEFs,也阻断JNK1/2和p38α MAPK磷酸化。BX795抑制TBK1/IKKε不会导致JNK1/2和p38α MAPK的激活。[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
THP1 MmmySpVv[3Srb36gZZN{[Xl? NH3yUJMyKGi{ NHjDW4xKdmirYnn0bY9vKG:oIHPHRXMhcW5iaIXtZY4hXEiSMTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4he2GubX;uJJNx\XKvIHTzSG5CNWmwZIXj[YQhUU[QLXLleIEh\XiycnXzd4lwdiCycnXpcoN2[mG2ZXSg[o9zKDFiaIKg[o9tdG:5ZXSgZpkh\HOGTlGgd5RqdXWuYYTpc44h\m:{IEGyJIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUCgQUAxNjB4IN88UU4> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl|NEK0Okc,Ojh7M{SyOFY9N2F-
KRX M1zob2Z2dmO2aX;uJIF{e2G7 NETiUYg4KG2rboO= MV\Jcohq[mm2aX;uJI9nKFOXTV:teIFo\2WmIGXMT|EhMHWwa37ve44hd3KrZ3nuLUBscW6jc3Wg[I9u[WmwIHX4dJJme3OnZDDpckBMWlhiY3XscJMhfXOrbnegX|MzWF1vZ3HtcYEuSVSSIHHu[EBugWWuaX6gZoF{cWNicILveIVqdiC|dXLzeJJifGViaX7jeYJifGWmIH\vdkA4KG2rboOsJGlEPTBiPTCwMlA5PyEQvF2u MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5NU[4NUc,OjZ{N{W2PFE9N2F-
KRX M1nkSGZ2dmO2aX;uJIF{e2G7 M{O0blchdWmwcx?= NEPUWYhKdmirYnn0bY9vKG:oIGPVUW8ufGGpZ3XkJHVNUzJiKIXub45wf25ib4Lp[4lvMSCtaX7hd4Uh\G:vYXnuJIV5eHKnc4Pl[EBqdiCNUmigZ4VtdHNidYPpcochYzN{UG2t[4FudWFvQWTQJIFv\CCveXXsbY4h[mG|aXOgdJJwfGWrbjDzeYJ{fHKjdHWgbY5kfWKjdHXkJIZweiB5IH3pcpMtKEmFNUCgQUAxNjNzIN88UU4> NFPuRVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3OVY5OSd-Mk[yO|U3QDF:L3G+
HeLa NH\MZ4NHfW6ldHnvckBie3OjeR?= NWfTNGRbPCC3TR?= NGHudIMzPCCqcoO= NGfNRZFKdmirYnn0bY9vKG:oIHH1eI9xcGGpeTDpckBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDMR|MuUSCycn;0[YlvKGGlY4XteYxifGmxbjDheEA1KHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KGmvbYXuc4Jtd3S2aX7nJI1mfGixZB?= NYjH[WlYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yO|U3QDFpPkK2Nlc2PjhzPD;hQi=>
TC32 NE\vV2RyUFSVIHHzd4F6 NWDnSGYxeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGTDN|Ih[2WubIO= Mlr5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
A673 NXjNNGtDeUiWUzDhd5NigQ>? MWfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? MkXtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
DAOY NFmye|RyUFSVIHHzd4F6 MUXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhTEGRWTDj[Yxtew>? M2nYe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 NFnh[Y5yUFSVIHHzd4F6 MkTzdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?= NYP6c|RVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-37 NVqy[I9ueUiWUzDhd5NigQ>? NVTScGR6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz MlKxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
RD NVHZPYJzeUiWUzDhd5NigQ>? MnjDdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ M4rUZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-SH M4nxfZFJXFNiYYPzZZk> M4\QWZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMXNJKGOnbHzz NUjmNYhRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-12 NUnaUJYyeUiWUzDhd5NigQ>? NIX1SIlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO= NXW2TZhqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
MG 63 (6-TG R) NUH3T4p6eUiWUzDhd5NigQ>? NF60VFdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTVegOlMhMDZvVFegVkkh[2WubIO= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 M3\vR5FJXFNiYYPzZZk> NVfqNHlNeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsdy=> NH:4NmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 MljKdWhVWyCjc4PhfS=> MV;xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| M1TJVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh41 NU\KXYRPeUiWUzDhd5NigQ>? MUXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? NE\Ybng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SJ-GBM2 MXXxTHRUKGG|c3H5 NX3jW2k5eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPKMWdDVTJiY3XscJM> Mn7rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC MVPxTHRUKGG|c3H5 M4HSfJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB-EBc1 NXrBdYZFeUiWUzDhd5NigQ>? NXOyfWhDeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM> NUTRemt2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
LAN-5 MX\xTHRUKGG|c3H5 MY\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? Mn73QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh18 NVvONod6eUiWUzDhd5NigQ>? M4DIZZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy| M33VcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MV-4-11 NGHrclhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2H5S2lvcGmkaYTpc44hd2ZiaIXtZY4hVVZvND2xNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC5yM{[4JO69VS5? MlrSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc4Pzh2Lze+V2FPT0WUPD;hQi=>
NKM-1 MmrOS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmTETY5pcWKrdHnvckBw\iCqdX3hckBPU01vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyIE2gNE4{PjF7NDFOwG0v NVGz[2NZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTd5N{i0M{c,W0GQR1XSQE9iRg>?
P30-OHK Mn74S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkHlTY5pcWKrdHnvckBw\iCqdX3hckBROzBvT1jLJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjR7NkWg{txONg>? MoLtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc4Pzh2Lze+V2FPT0WUPD;hQi=>
YH-13 MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NXHVU5NSUW6qaXLpeIlwdiCxZjDoeY1idiC\SD2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC54Mki4PEDPxE1w M4LIb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFU4Pzd6ND:nQnNCVkeHUkyvZV4>
LXF-289 NUn2O4hoT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M{nid2lvcGmkaYTpc44hd2ZiaIXtZY4hVFiILUK4PUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC54NU[5N{DPxE1w NEDSUIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2Pzd5OESvK|5USU6JRWK8M4E,
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-p38 / p38 / p-JNK / JNK / gD-2 ; 

PubMed: 28112176     


BX-795 inhibition on HSV replication attenuated by overexpression of p38/JNK. HeLa cells with or without JNK1 or p38 overexpression were infected with HSV-2 and total cellular protein was prepared for WB analysis. gD-2, the phosphorylation level of p38 MA䲧疝Ỵ疞㧀疜膉痘 瘿뙠ෆᾰƌෆĀ 㺣痖帉痖Ѐ瑖堘

pTBK1 / TBK1 / pPLK1 / PLK1; 

PubMed: 26772203     


TBK1 mediated activation of the mitotic kinase PLK1. SW210.5 cells were treated with indicated TBK1 inhibitors for 1 hour and then exposed to 50 ng/mL of nocodazole for 18 hours. Western blot detected the signaling change using indicated antibodies, and β䲧疝Ỵ疞㧀疜膉痘 瘿⟸෕ᾰƌ෕Ð 㺣痖帉痖Ѐ

28112176 26772203
Growth inhibition assay
Cell viability ; 

PubMed: 26772203     


Four SCLC cell lines with no MYC amplification were treated with TBK1 inhibitor BX-795 at the titration concentration. Cell viability was performed by CellTiter Glo 4 days after treatment. The growth curve was constructed by GraphPad Prism 6. 

26772203

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

蛋白激酶实验:

加入[γ-32P]ATP(1000 cpm/pmol)开始实验,终浓度为0.1 mM。使用GST-IRF3作为底物, BX795抑制反应在30oC下进行30分钟,加入含40 mM EDTA,pH 为7.0的SDS终止反应,在100oC下加热5分钟,通过SDS-PAGE分离, 然后通过放射自显影技术测定磷酸化的蛋白。通过感光成像分析进行量化处理。IκBα底物肽实验在30oC下进行10分钟, 使一部分反应物放到一张2×2-cm2浸泡在75 mM磷酸中的磷酸纤维素P81纸上,终止反应。在磷酸中冲洗6次,在丙酮中冲洗1次,烘干纸,计数。
细胞实验:[3]
- 合并
  • Cell lines: MDA-468, MDA-453, HCT-116, U87-MG
  • Concentrations: 0.001-1 μM
  • Incubation Time: 72小时
  • Method: 细胞系包括MDA-468,MDA-453,HCT-116,U87-MG等按每孔1.5-3×103个细胞接种在96孔板上,过夜。每孔加入溶于1% DMSO和生长培养基(DMSO最终浓度为0.1%)的10 μL BX795, 震荡处理。细胞温育72小时,加入10 μL代谢染料测定活力。在450 nm处测定WST-1信号。
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL (169.07 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG +2% Tween 80+ddH2O
4mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 591.47
化学式

C23H26IN7O2S

CAS号 702675-74-9
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Can you suggest an in vivo formulation of S1274 for animal studies?

  • 回答:

    We recommend to use the vehicle 2% DMSO+30% PEG +2% Tween 80+ddH2O for in vivo study.

PDK Signaling Pathway Map

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