BX-795

For research use only. Not for use in humans.

目录号:S1274

BX-795 Chemical Structure

CAS No. 702675-74-9

BX-795 是一种有效的,特异性的PDK1抑制剂,在无细胞试验中IC50为6 nM,作用于PDK1比作用于PKA和PKC选择性分别高140和1600倍。同时,相比于 GSK3β,作用于PDK1的选择性高出100多倍。BX-795 可通过抑制 ULK1 来调节自噬。BX-795 还是一种有效的 TBK1 抑制剂,可阻断 TBK1 和 IKKε,IC 50 值分别为 6 nM 和 41 nM。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1628.91 现货
RMB 1236.66 现货
RMB 2623.21 现货
RMB 4662.25 现货
RMB 7931.08 现货
有超大折扣
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客户使用Selleck生产的BX-795发表文献57篇:

产品安全说明书

PDK抑制剂选择性比较

生物活性

产品描述 BX-795 是一种有效的,特异性的PDK1抑制剂,在无细胞试验中IC50为6 nM,作用于PDK1比作用于PKA和PKC选择性分别高140和1600倍。同时,相比于 GSK3β,作用于PDK1的选择性高出100多倍。BX-795 可通过抑制 ULK1 来调节自噬。BX-795 还是一种有效的 TBK1 抑制剂,可阻断 TBK1 和 IKKε,IC 50 值分别为 6 nM 和 41 nM。
靶点
PDK-1 [1]
(Cell-free assay)
c-Kit [1]
(Cell-free assay)
CDK2/CyclinE [1]
(Cell-free assay)
Chk1 [1]
(Cell-free assay)
6 nM 320 nM 430 nM 510 nM
体外研究

BX795也抑制MARK1,MARK2,MARK4,NUAK1,VEGFR,MLK1,MLK2,和MLK3,IC50分别为55,53,19,5,157,50,46和42 nM。1 μM BX795不抑制如下酪氨酸蛋白激酶: 肝配蛋白受体A2和B3,Syk,Bruton's酪氨酸激酶,和FGFR1。然而,BX795抑制VEGFR,抑制效果比TBK1低。BX795抑制TBK1-催化的IRF3在Ser396位点磷酸化随着ATP浓度上升而下降,说明BX795是ATP竞争性抑制剂。BX795阻断TBK1和IKKε调节的IRF3激活,且抑制IFN-β的产量。聚(I:C)处理后,IRF3在核中积累,但是可被BX795抑制。 BX795抑制IRF3依赖的基因转录。BX795抑制IFN-β从巨噬细胞中分泌。BX795对LPS刺激的p70核糖体S6激酶1在Thr229位点磷酸化没有效果, Thr229位点是PDK1作用靶点。BX795不影响IKKα/β复合体或LPS,聚(I:C),IL-1α,或TNFα促进的NFκB依赖的基因转录的激活。BX795作用于IL-1α或TNFα刺激的MEFs,也阻断JNK1/2和p38α MAPK磷酸化。BX795抑制TBK1/IKKε不会导致JNK1/2和p38α MAPK的激活。[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
THP1 MXvGeY5kfGmxbjDhd5NigQ>? MnzRNUBpeg>? NUixV3N4UW6qaXLpeIlwdiCxZjDjS2FUKGmwIHj1cYFvKFSKUEGgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKHOjbH3vckB{eGW{bTDkd2RPSS2rbnT1Z4VlKEmITj3i[ZRiKGW6cILld5Nqd25icILlbY5kfWKjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KGS|RF7BJJN1cW23bHH0bY9vKG[xcjCxNkBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC5yNjFOwG0v MonRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7M{SyOFYoRjJ6OUO0NlQ3RC:jPh?=
KRX MYLGeY5kfGmxbjDhd5NigQ>? NYTtb2tOPyCvaX7z MXLJcohq[mm2aX;uJI9nKFOXTV:teIFo\2WmIGXMT|EhMHWwa37ve44hd3KrZ3nuLUBscW6jc3Wg[I9u[WmwIHX4dJJme3OnZDDpckBMWlhiY3XscJMhfXOrbnegX|MzWF1vZ3HtcYEuSVSSIHHu[EBugWWuaX6gZoF{cWNicILveIVqdiC|dXLzeJJifGViaX7jeYJifGWmIH\vdkA4KG2rboOsJGlEPTBiPTCwMlA5PyEQvF2u MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5NU[4NUc,OjZ{N{W2PFE9N2F-
KRX MWLGeY5kfGmxbjDhd5NigQ>? MV[3JI1qdnN? MYDJcohq[mm2aX;uJI9nKFOXTV:teIFo\2WmIGXMT|IhMHWwa37ve44hd3KrZ3nuLUBscW6jc3Wg[I9u[WmwIHX4dJJme3OnZDDpckBMWlhiY3XscJMhfXOrbnegX|MzWF1vZ3HtcYEuSVSSIHHu[EBugWWuaX6gZoF{cWNicILveIVqdiC|dXLzeJJifGViaX7jeYJifGWmIH\vdkA4KG2rboOsJGlEPTBiPTCwMlMyKM7:TT6= MofjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{W2PFEoRjJ4Mke1OlgyRC:jPh?=
HeLa NVK3[XFUTnWwY4Tpc44h[XO|YYm= Mk\KOEB2VQ>? NFnITo4zPCCqcoO= NXrjc4NNUW6qaXLpeIlwdiCxZjDheZRweGijZ4mgbY4hcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gUGM{NUlicILveIVqdiCjY3P1cZVt[XSrb36gZZQhPCC3TTDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCrbX31co9jdG:2dHnu[{Bu\XSqb3S= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJ5NU[4NUc,OjZ{N{W2PFE9N2F-
TC32 MWLxTHRUKGG|c3H5 NUL6XnF4eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGTDN|Ih[2WubIO= NF;Z[HA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
A673 M3\Pc5FJXFNiYYPzZZk> M3rUbpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy| Mn:wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
DAOY MUHxTHRUKGG|c3H5 MW\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhTEGRWTDj[Yxtew>? M1ryd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 M3z0SZFJXFNiYYPzZZk> M33TbpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVYX;zMVIh[2WubIO= MoHnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 M320UZFJXFNiYYPzZZk> MmrsdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUO3JINmdGy| M4To[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD MkfadWhVWyCjc4PhfS=> NHjPcm5yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUlSgZ4VtdHN? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-SH M4X3cZFJXFNiYYPzZZk> NWr6WJJ{eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uW0hiY3XscJM> M1PncVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-12 M{DydJFJXFNiYYPzZZk> NYrsbnp4eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVEzKGOnbHzz NWfEXWdFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
MG 63 (6-TG R) MoX2dWhVWyCjc4PhfS=> MnfZdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy| M2rXe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MnnRdWhVWyCjc4PhfS=> NHzSXpNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
OHS-50 NIrpW3lyUFSVIHHzd4F6 MYrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 MYnxTHRUKGG|c3H5 NXHrZXAyeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoOFEh[2WubIO= Mn74QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 NFnnNY1yUFSVIHHzd4F6 MmfsdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= MkizQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC NX;ZT45CeUiWUzDhd5NigQ>? NYrxfHhReUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> NEXJcHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 M3TsV5FJXFNiYYPzZZk> M2XXOpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQj3FRoMyKGOnbHzz NHjxOFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 NUfUXVA3eUiWUzDhd5NigQ>? MYfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? NX;sdnliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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MV-4-11 M1rTT2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWfzXJZ1UW6qaXLpeIlwdiCxZjDoeY1idiCPVj20MVEyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSByLkCzOlgh|ryPLh?= NXnLbIxQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTd5N{i0M{c,W0GQR1XSQE9iRg>?
NKM-1 MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NG\kNVJKdmirYnn0bY9vKG:oIHj1cYFvKE6NTT2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUCgQUAxNjN4MUm0JO69VS5? NF7nNJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2Pzd5OESvK|5USU6JRWK8M4E,
P30-OHK MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWjGd5ZIUW6qaXLpeIlwdiCxZjDoeY1idiCSM{CtU2hMKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVAhRSByLkS5OlUh|ryPLh?= MknVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc4Pzh2Lze+V2FPT0WUPD;hQi=>
YH-13 NEjY[HNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4L3VWlvcGmkaYTpc44hd2ZiaIXtZY4hYUhvMUOgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFAvPjJ6OEig{txONg>? NUHIdoZDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTd5N{i0M{c,W0GQR1XSQE9iRg>?
LXF-289 MYfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH\sTIJKdmirYnn0bY9vKG:oIHj1cYFvKEy[Rj2yPFkh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDBwNkW2PVMh|ryPLh?= Mlm5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVc4Pzh2Lze+V2FPT0WUPD;hQi=>
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-p38 / p38 / p-JNK / JNK / gD-2 ; 

PubMed: 28112176     


BX-795 inhibition on HSV replication attenuated by overexpression of p38/JNK. HeLa cells with or without JNK1 or p38 overexpression were infected with HSV-2 and total cellular protein was prepared for WB analysis. gD-2, the phosphorylation level of p38 MA䲧疝Ỵ疞㧀疜膉痘瘿뙠ෆᾰƌෆĀ㺣痖帉痖Ѐ瑖堘

pTBK1 / TBK1 / pPLK1 / PLK1; 

PubMed: 26772203     


TBK1 mediated activation of the mitotic kinase PLK1. SW210.5 cells were treated with indicated TBK1 inhibitors for 1 hour and then exposed to 50 ng/mL of nocodazole for 18 hours. Western blot detected the signaling change using indicated antibodies, and β䲧疝Ỵ疞㧀疜膉痘瘿⟸෕ᾰƌ෕Ð㺣痖帉痖Ѐ

28112176 26772203
Growth inhibition assay
Cell viability ; 

PubMed: 26772203     


Four SCLC cell lines with no MYC amplification were treated with TBK1 inhibitor BX-795 at the titration concentration. Cell viability was performed by CellTiter Glo 4 days after treatment. The growth curve was constructed by GraphPad Prism 6. 

26772203

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

蛋白激酶实验:

加入[γ-32P]ATP(1000 cpm/pmol)开始实验,终浓度为0.1 mM。使用GST-IRF3作为底物, BX795抑制反应在30oC下进行30分钟,加入含40 mM EDTA,pH 为7.0的SDS终止反应,在100oC下加热5分钟,通过SDS-PAGE分离, 然后通过放射自显影技术测定磷酸化的蛋白。通过感光成像分析进行量化处理。IκBα底物肽实验在30oC下进行10分钟, 使一部分反应物放到一张2×2-cm2浸泡在75 mM磷酸中的磷酸纤维素P81纸上,终止反应。在磷酸中冲洗6次,在丙酮中冲洗1次,烘干纸,计数。
细胞实验:[3]
- 合并
  • Cell lines: MDA-468, MDA-453, HCT-116, U87-MG
  • Concentrations: 0.001-1 μM
  • Incubation Time: 72小时
  • Method: 细胞系包括MDA-468,MDA-453,HCT-116,U87-MG等按每孔1.5-3×103个细胞接种在96孔板上,过夜。每孔加入溶于1% DMSO和生长培养基(DMSO最终浓度为0.1%)的10 μL BX795, 震荡处理。细胞温育72小时,加入10 μL代谢染料测定活力。在450 nm处测定WST-1信号。
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL (169.07 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG +2% Tween 80+ddH2O
4mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 591.47
化学式

C23H26IN7O2S

CAS号 702675-74-9
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Can you suggest an in vivo formulation of S1274 for animal studies?

  • 回答:

    We recommend to use the vehicle 2% DMSO+30% PEG +2% Tween 80+ddH2O for in vivo study.

PDK Signaling Pathway Map

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