Caffeic Acid Phenethyl Ester

别名: CAPE, Phenylethyl Caffeate 中文名称:咖啡酸苯乙酯

Caffeic acid phenethyl ester (CAPE, Phenylethyl Caffeate)是强效的特异性NF-κB活化抑制剂,也能够表现出抗氧化、免疫调节和抗炎活动。

Caffeic Acid Phenethyl Ester Chemical Structure

Caffeic Acid Phenethyl Ester Chemical Structure

CAS: 104594-70-9

规格 价格 库存 购买数量
50mg RMB 808.46 现货
200mg RMB 2213.96 现货
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批次: 纯度: 99.99%
99.96

Caffeic Acid Phenethyl Ester相关产品

相关信号通路图

NF-κB抑制剂选择性比较

细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
rat VSMC Function assay 5 μM 30 mins Decrease in intracellular ROS level in PDGF-stimulated rat VSMC at 5 uM pretreated with compound for 30 mins measured after up to 23 hrs of post stimulation using H2DCF by flow cytometric analysis 21265554
rat PC12 cells Function assay 2.5 μM 3 h Neuroprotective activity against H2O2-induced damage in rat PC12 cells assessed as cell viability at 2.5 uM preincubated for 3 hrs followed by H2O2 challenge measured after 5 hrs by MTT assay 24697335
mouse RAW264.7 cells Function assay 24 h Inhibition of LPS-induced nitric oxide production in mouse RAW264.7 cells assessed as nitrite accumulation administered 1 hr before LPS challenge and measured after 24 hrs by Griess reagent assay, EC50=0.193 μM 18667320
mouse 26-L5 cells Proliferation assay 72 h Antiproliferative activity against mouse 26-L5 cells after 72 hrs by MTT assay, EC50=0.3 μM 12027739
human HeLa cells Proliferation assay 72 h Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay, EC50=2.36 μM 12027739
mouse J774.1 cells Function assay 24 h Inhibition of LPS-induced NO production in mouse J774.1 cells after 24 hrs by Griess reagent assay, IC50=4.8 μM 15974608
human LNCAP cells Function assay 24 h Antiandrogenic activity in human LNCAP cells assessed as reduction in DHT-stimulated PSA release after 24 hrs by ELISA, IC50=6.2 nM 24080105
mouse BV2 cells Function assay 24 h Inhibition of nitric oxide production in LPS-stimulated mouse BV2 cells treated 3 hrs before LPS addition measured after 24 hrs by Griess assay, IC50=6.4 μM 23870700
mouse B16-BL6 cells Proliferation assay 72 h Antiproliferative activity against mouse B16-BL6 cells after 72 hrs by MTT assay, EC50=6.79 μM 12027739
HUVEC cells Cytotoxic assay 18 h Cytoprotective activity against H2O2-induced oxidative stress in HUVEC cells after 18 hrs by Cell-Titer Blue assay 20598894
human HT1080 cells Proliferation assay 72 h Antiproliferative activity against human HT1080 cells after 72 hrs by MTT assay, EC50=9.5 μM 12027739
human HL60 cells Cytotoxic assay 72 h Cytotoxicity against human HL60 cells after 72 hrs by MTT assay, IC50=9.8 μM 20673727
human LS 174T cells Cytotoxic assay 72 h Cytotoxicity against human LS 174T cells after 72 hrs by MTT assay, IC60=9.9 μM 20673727
human Bel7404 cells Cytotoxic assay 72 h Cytotoxicity against human Bel7404 cells after 72 hrs by MTT assay, IC50=14.5 μM 20673727
human BGC823 cells Cytotoxic assay 72 h Cytotoxicity against human BGC823 cells after 72 hrs by MTT assay, IC50=19.3 μM 20673727
human HO8910 cells Cytotoxic assay 72 h Cytotoxicity against human HO8910 cells after 72 hrs by MTT assay, IC50=25.5 μM 20673727
human A549 cells Cytotoxic assay 72 h Cytotoxicity against human A549 cells after 72 hrs by MTT assay, IC50=26.8 μM 20673727
human LNCAP cells Cytotoxic assay 24 h Cytotoxicity against human LNCAP cells assessed as reduction in cell viability after 24 hrs by WST-1 assay, IC50=33.7 μM 24080105
human ECA109 cells Cytotoxic assay 72 h Cytotoxicity against human ECA109 cells after 72 hrs by MTT assay, IC50=42.6 μM 20673727
human KB cells Cytotoxic assay 72 h Cytotoxicity against human KB cells after 72 hrs by MTT assay, IC50=45.2 μM 20673727
human K562 cells Cytotoxic assay 72 h Cytotoxicity against human K562 cells after 72 hrs by MTT assay, IC50=46 μM 20673727
human Bewo cells Function assay Antitumor activity against human Bewo cells assessed as inhibition of cell growth by MTT assay, IC50=2.96 μM 25160837
human Bel7402 cells Cytotoxic assay Cytotoxicity against human Bel7402 cells by MTT assay, IC50=5.5 μM 18952420
human SGC7901 cells Function assay Antitumor activity against human SGC7901 cells assessed as inhibition of cell growth by MTT assay, IC50=14.26 μM 25160837
human MCF7 cells Cytotoxic assay Cytotoxicity against human MCF7 cells by MTT assay, IC50=26.7 μM 18952420
human HeLa cells Function assay Antitumor activity against human HeLa cells assessed as inhibition of cell growth by MTT assay, IC50=37.98 μM 25160837
human HepG2 cells Function assay Antitumor activity against human HepG2 cells assessed as inhibition of cell growth by MTT assay, IC50=40.87 μM 25160837
human BCG823 cells Cytotoxic assay Cytotoxicity against human BCG823 cells by MTT assay, IC50=44.6 μM 18952420
点击查看更多细胞系数据

生物活性

产品描述 Caffeic acid phenethyl ester (CAPE, Phenylethyl Caffeate)是强效的特异性NF-κB活化抑制剂,也能够表现出抗氧化、免疫调节和抗炎活动。
靶点
NF-κB [1]
体外研究(In Vitro)
体外研究活性 Caffeic acid phenethyl ester能够通过抑制p65的入核转运来抑制佛波酯,神经酰胺,强心酸过氧化氢等诱导的NF-kB的激活。[1] 在一系列的肿瘤细胞系中,Caffeic acid phenethyl ester具有抗增殖的活性,其对26-L5, B16-BL6恶性瘤, 人类HT-1080的 EC50 分别是 1.76, 3.16, 13.7,和44.0 μM。[2] Caffeic acid phenethyl ester通过抑制ROS的形成和抑制半胱氨酸天冬氨酸蛋白酶的活性发挥抗凋亡的作用。Caffeic acid phenethyl ester通过抑制NF-kB信号通路来减弱LPS诱导的炎症和LPS诱导的HSCs细胞对细胞因子的敏感性。[4]
细胞实验 细胞系 小鼠结肠 26-L5, 小鼠B16-BL6黑色素瘤,人类HT-1080 纤维肉瘤和人类肺 A549 腺癌细胞系。
浓度 ~200 μM
孵育时间 3天
方法

人HT-1080纤维肉瘤,人肺癌A549腺癌细胞和鼠B16-BL6黑色素瘤细胞在含有10%的血清,0.1%碳酸盐,和2mM的谷氨酰胺的EMEM培养基中培养。鼠结肠癌细胞 B16-BL6用含有相同添加成分的RPMI中培养。除了A-549以外,这些都是有高度侵染能力的细胞系。细胞存活实验采用标准的MTT方法。简单的说,对数生长的细胞收集以后以2000个每孔的密度种植于96孔板。24小时孵育后,用含有不同浓度的样品的100ul培养基处理细胞,5%的二氧化碳37度条件下培养72小时。加入MTT3小时以后用Perkin Elmer HTS-7000测定550纳米波长的吸收值。待测样品先用DMSO溶解,再用培养基稀释。DMSO的终浓度小于0.25%。对照组还有相同浓度的DMSO。5-氟尿嘧啶(5-FU) 和 doxorubicin HCl用作阳性对照。EC50值通过取4个孔数据的平均值来计算的。

实验图片 检测方法 检测指标 实验图片 PMID
Western blot p-p65 / CDH6 / RUNX2 / E-cadherin / N-cadherin / Vimentin / Slug / Snail Nrf2 AKT / AKT1 / AKT2 / AKT3 / p-AKT / FOXO1 / FOXO3a / p-FOXO1 / p-FOXO3a / Skp2 / p27(Kip) / Cyclin D1 / Rb / p-Rb 29284791
Immunofluorescence NF-κB 29284791
Growth inhibition assay Cell number 23615471
体内研究(In Vivo)
体内研究活性 C57BL/6 and BALB/c小鼠腹腔内注射10毫克/千克Caffeic acid phenethyl ester能够抑制在路易斯肺细胞癌,结肠癌,和黑色素瘤的生长和肿瘤的血管新生。[5]在动物体内(5, 10, 20 mg/kg) 的Caffeic acid phenethyl ester通过减少胸腺的重量和胸腺和脾的孔性而具有免疫调节的作用。[6]
动物实验 Animal Models 接种Lewis肺肿瘤,结肠癌以及黑色素瘤细胞的C57BL/6 和 BALB/c 小鼠
Dosages ~10毫克/千克
Administration 腹腔注射
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05934019 Recruiting
Mental Health Issue|Transdiagnostic Mechanisms|Adolescent Psychology|Prevention|Online Intervention|Psychological Intervention|Mental Disorder in Adolescence Subclinical
University of Bern
June 9 2023 Not Applicable
NCT05726708 Not yet recruiting
Autism Spectrum Disorder
Centre Hospitalier Universitaire Vaudois
March 1 2023 Not Applicable
NCT05157828 Completed
Iron Deficiency Anemia of Pregnancy
University of Cape Town
January 31 2022 --
NCT04633434 Recruiting
Parenting Practices|Self Efficacy|Stress|Child Behavior|Emotional Adjustment|Sleep Problems|Parent-Child Relations
Oregon Research Behavioral Intervention Strategies Inc.|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
September 1 2021 Not Applicable

化学信息&溶解度

分子量 284.31 分子式

C17H16O4

CAS号 104594-70-9 SDF Download Caffeic Acid Phenethyl Ester SDF
Smiles C1=CC=C(C=C1)CCOC(=O)C=CC2=CC(=C(C=C2)O)O
储存条件(自收到货起)

体外溶解度
批次:

DMSO : 56 mg/mL ( 196.96 mM; DMSO吸湿会降低化合物溶解度,请使用新开封DMSO)

Ethanol : 56 mg/mL

Water : Insoluble

摩尔浓度计算器

体内溶解度
批次:

现配现用,请按从左到右的顺序依次添加,澄清后再加入下一溶剂

动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

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