BAY 11-7082

For research use only. Not for use in humans.

目录号:S2913 别名: BAY 11-7821

BAY 11-7082 Chemical Structure

CAS No. 19542-67-7

BAY 11-7082 (BAY 11-7821)是一种 NF-κB 抑制剂,抑制TNFα诱导的 IκBα 磷酸化,在肿瘤细胞中IC50为10 μM。BAY 11-7082 可抑制 ubiquitin-specific protease USP7USP21,对应的IC50值分别为0.19 μM和0.96 μM。BAY 11-7082 在胃癌细胞中可诱导凋亡和S期阻滞。

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RMB 738.58 现货
RMB 2223.41 现货
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客户使用Selleck生产的BAY 11-7082发表文献184篇:

产品安全说明书

IκB/IKK抑制剂选择性比较

生物活性

产品描述 BAY 11-7082 (BAY 11-7821)是一种 NF-κB 抑制剂,抑制TNFα诱导的 IκBα 磷酸化,在肿瘤细胞中IC50为10 μM。BAY 11-7082 可抑制 ubiquitin-specific protease USP7USP21,对应的IC50值分别为0.19 μM和0.96 μM。BAY 11-7082 在胃癌细胞中可诱导凋亡和S期阻滞。
靶点
E2-conjugating enzymes [6]
(Cell-free assay)
IκBα phosphorylation [1]
(Tumor cells)
10 μM
体外研究

BAY 11-7082完全且特异性地废除 NF-κB DNA结合, 下调NF-κB-诱导的细胞因子IL-6,且诱导细胞凋亡。[1] BAY 11-7082 (< 8 μM) 有效抑制原有的和TNFα 刺激的NF-κB荧光素酶活性,这种作用存在剂量依赖性。BAY 11-7082 (8 μM) 强抑制NCI-H1703 细胞增殖。 [2] Bay 11-7082 (5 μM)作用于感染HTLV-I的T细胞系,快速且有效的降低NF-κB的DNA结合力,且下调抗凋亡基因Bcl-x(L)的表达,而对另一种转录因子AP-1与DNA结合没有作用效果。Bay 11-7082诱导原代ATL细胞发生凋亡比正常的外周血单核细胞更明显,而诱导的这些细胞凋亡也与NF-κB活性的下调相关。Bay 11-7082 (5 μM) 选择性诱导感染HTLV-I的T细胞系发生细胞凋亡,且下调 cyclin D1, cyclin D2, 和 Bcl-xL的表达。 [3] BAY 11-7082 (100 μM)作用于小鼠海马脑片区,抑制NMDA引起的p65发生 核易位,也抑制NMDA诱导的NF-κB结合力的增强。BAY 11-7082抑制NMDA毒性发生在海马脑片区的CA1区,20 μM时,对40%神经有保护作用,100 μM时,对70%神经有保护作用。[4]作用于脂肪组织,实验中所有浓度的BAY 11-7082都显著抑制 NF-κB p65 DNA结合活性,而作用于骨骼肌,只有50 μM 和 100 μM 的BAY 11-7082显著抑制NF-κB p65 DNA结合活性。BAY 11-7082 (100 μM) 作用于人体脂肪组织和骨骼肌,降低IKK-β蛋白。BAY 11-7082 (100 μM) 显著降低TNF-α从动物脂肪组织中释放,而 IL-6 和 IL-8 的释放显著抑制实验中BAY 11-7082的所有浓度。 BAY 11-7082 (50 μM)作用于骨骼肌,显著降低 TNF-α, IL-6, 和IL-8 的释放。[5] 此外,BAY 11-7082还被发现抑制了 E2-conjugating酶和E3 ligase酶的活性,从而诱导了B-淋巴癌细胞和T淋巴癌细胞的死亡。 [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa NH31WIVHfW6ldHnvckBCe3OjeR?= MXexNOKh|ryP MV6xMlUhcA>? NF3hR|Bi[m:uaYPo[ZMhSlCDIHnu[JVk\WRidYCgdoVofWyjdHnvckBw\iCITjDhcoQhVU2SLUpCpC=> Mkm5NlU4QTd2M{e=
SiHa  M2TDVGZ2dmO2aX;uJGF{e2G7 NILKPJEyOMLizszN NXL6cW94OS53IHi= NVjLbXN7[WKxbHnzbIV{KEKSQTDpcoR2[2WmIIXwJJJm\3WuYYTpc44hd2ZiRl6gZY5lKE2PUD25xsA> NEX2d|QzPTd7N{SzOy=>
ARPE-19 NU\2dIZITnWwY4Tpc44hSXO|YYm= M2q5XVEh|ryP NFPFOZcxNjViaB?= MljHd5VxeHKnc4Pld{BVTy2rbnT1Z4VlyqCLTD24xsBxem:vb4TldkBi[3SrdnH0bY9v MlH6NlU2QTNyMkm=
HCT116 MnW5SpVv[3Srb36gRZN{[Xl? NVj3TYRyPSEQvF2= MnSyNkBp M{fGPGROW09? NXTLSno2[XS2ZX71ZZRmeyC|aXz5cYFzcW5vaX7keYNm\CCmb4fudoVofWyjdHnvckBw\iCleXPsbY4hTDF? MVuyOVQ4QTd{Mx?=
HMECs M3rQNWZ2dmO2aX;uJGF{e2G7 NFG0[2Y2yqEQvF2= MWWyJIg> MX3hZo9tcXOqZYOgWG5HNc7zLXnu[JVk\WRiVlPBUU0yKGW6cILld5Nqd28EoB?= MXuyOVE6OzFzNh?=
A549  NFf2XolHfW6ldHnvckBCe3OjeR?= MkfQNVDjiIoEtV2= Mn3ONVIhcA>? NFfMOZV{fXCycnXzd4V{KES4bD2zJIlv\HWlZXSgZYN1cX[jdHnvckBw\iCyNkW= MmfCNlUyPTZ6MEC=
RAW 264.7 M{DDemZ2dmO2aX;uJGF{e2G7 M1\Id|XDqM7:TR?= MnOwNUBp M4T6dolvcGmkaYTzJHRPTi4QsTDhcoQhUUxvMUKgdFQxKHC{b3T1Z5Rqd25? MYeyOVAyQTV4Nx?=
macrophages MWPGeY5kfGmxbjDBd5NigQ>? NID4b3o2KML3TR?= NInXUpA{KGh? MVHwZZJ1cWGubImgZoxw[2u|IGnQSnMucW6mdXPl[EBmgHC{ZYPzbY9vKG:oIHnOU3Mh[W6mIFPPXE0zyqB? M1PZcVI1QTZ5OEm4
HUVECs NF\BbZVHfW6ldHnvckBCe3OjeR?= NX[yN4tbOy1|MDFOwG0> M3nGflEhcA>? NWLVXHpWemWmdXPld{B1cGViZYjwdoV{e2mxbjDv[kBucVJvMUS2ZUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NUS1bVNmOjR6NkO5OlU>
HeLa  MWXGeY5kfGmxbjDBd5NigQ>? NXnLUW9LPcLizszN MWOyOEBp NETxT|RFVVOR Ml7rdoVlfWOnczD0bIUh[WO2aY\peJkhd2ZiVF7GMe6yKHC{b33veIVzyqB? MoTaNlQ3PTd5OEO=
A549 NFT0R3dHfW6ldHnvckBCe3OjeR?= MlXsNVAh|ryP MoqwNgKBkWh? MXLpcohq[mm2czD0bIUhcW6lcnXhd4Uhd2ZicHjvd5Bpdy2LzsrC{tEhcW5iUFGxNFMucW6oZXP0[YQh[3WudIXy[ZPDqA>? M2DGfFI1PjF{NEi4
HUVEC MXTGeY5kfGmxbjDBd5NigQ>? M{TCelIxKML3TR?= MnW2NE42KGh? MYnEUXNQ NXXBNJdSeHKndnXueJMhfGinIHnu[JVkfGmxbjDv[kBGSU1iZYjwdoV{e2mxbh?= MXmyOFU2OTJyOR?=
A549RT-eto MW\BdI9xfG:|aYOgRZN{[Xl? MVexNOKh|ryP NXPTTGZXOjRiaB?= Mo\KSG1UVw>? M{HNdIFk[2WuZYLheIV{KE[HUl:tcYVlcWG2ZXSgZZBweHSxc3nz M3vncVI1PTN3MEiz
THP-1  NX7wcIFvTnWwY4Tpc44hSXO|YYm= MoOzNE4yNzIEoN88US=> NIHVS4wxNjViaB?= M1\Ec4Fjem:pYYTld{BVVkZvzsGgd4VkemW2aX;uJIF{KHenbHygZZMhfGinIHnuZ5Jm[XOnZDDz[YNz\XSrb36gc4YhUUxvNjDhcoQhUUxvMd8y NIHFcZkzPDN5OEWzOi=>
SKCXCR2  NGjzTGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV2yJOK2VQ>? MWG0PEBp NFnPSHJl\WO{ZXHz[ZMh[2WubDDwdo9tcW[ncnH0bY9vKHOrZ37p[olk[W62bIm= MUWyOFM4Pjd2Nx?=
SKCXCR2  MVPGeY5kfGmxbjDBd5NigQ>? MXOyJOK2VQ>? NEfSfHY1QCCq MWLicI9kc3NidHjlJGNZS0xzLXnu[JVk\WRiY3XscEBqdn[jc3nvci=> M1roWlI1Ozd4N{S3
OVCXCR2 NGnkdFlHfW6ldHnvckBCe3OjeR?= MXWyJOK2VQ>? Mn;BOFghcA>? NVjHXnZv[myxY3vzJJRp\SCFWFPMNU1qdmS3Y3XkJINmdGxiaX72ZZNqd25? NVrNNlBjOjR|N{[3OFc>
DSCs  M4WzemZ2dmO2aX;uJGF{e2G7 NFHWO3ozNjVizszN MlroNE42KGh? MV7y[ZZmenOnczD0bIUh\W6qYX7j[Y1mdnRib3[gR2NNOi:FQ2KyJIV5eHKnc4Ppc44hd2ZiRGPDd{BqdmS3Y3XkJIJ6KEmOLUOz NVjWZ3NMOjR|NESyOFA>
WPs M2LFZ2Z2dmO2aX;uJGF{e2G7 MorzNlUh|ryP NVHwW3VMPSCvaX6= NGW5UIl{fXCycnXzd4V{KEGWUDDhcoQhfleIIIPlZ5JmfGmxbh?= NEX0[JkzPDN|MUKwOy=>
A549RT-eto M2jne2Fxd3C2b4Ppd{BCe3OjeR?= MnLtNVDDqM7:TR?= MWmyOEBp M162cIFk[2WuZYLheIV{KEZzNDDlfJRz[WO2LX3l[IlifGWmIHHwc5B1d3OrczDjc41jcW6nZDD0doVifG2nboSge4l1cCCIMUS= M1\0dVI1OjJyN{K1
A549RT-eto NUPN[2hnTnWwY4Tpc44hSXO|YYm= Ml\zNVDDqM7:TR?= M2LiRlI1KGh? M4D3[IRm[3KnYYPld{B1cGViZYjwdoV{e2mxbjDs[ZZmdHNib3[gUmYu|rqEIHHu[EBRNWeywrC= MW[yOFIzODd{NR?=
FaDu M{j0cmZ2dmO2aX;uJGF{e2G7 MlnjNkBp NXfkTpQ4cW6qaXLpeJMheDZ3IHX4dJJme3Orb36gZY5lKGKub3Prd{BVVkcQsT3pcoR2[2WmIGTXTXNVKGW6cILld5Nqd25? NEPHZ4szPDJ{ME[yNi=>
IVD  MmfuSpVv[3Srb36gRZN{[Xl? NYD5NpNNOTEEoN88US=> NULIVHBHOyCm MUPy[ZZmenOnczDUUmYu|rIkgKPt[YRq[XSnZDDzeZBxemW|c3nvckBw\iC2aHWg[Il{[yCvYYTybZghdWGlcn;tc4xm[3WuZYOgZYdoemWlYX6gZY5lKGOxbHzh[4VvKEmL M{j0XlI1OTd4OEC4
IVD  MYHGeY5kfGmxbjDBd5NigQ>? NF31e3kyOMLizszN MnXnN{Bl M1H3VoFjem:pYYTld{BVVkZvzsJihLNqdmS3Y3XkJJVxNXKnZ4XsZZRqd25ib3[gRWRCVVSVLUSgZY5lKEGGQV3UV{02yqB? NVTLTFl7OjRzN{[4NFg>
iNKT NW\tfItVTnWwY4Tpc44hSXO|YYm= MWOxNE8yODBizszN Mn;GNE42KGh? M{LkPIlvcGmkaYTzJJRp\SCrbnT1Z5Rqd25ib3[gRVJCWiCvUl7BJIFv\CCxdHjldkBn[WO2b4K= NELZeYszPDF{NES1Ny=>
PC-3 MV;GeY5kfGmxbjDBd5NigQ>? NIjqXJYzNjVxNT:xNEDPxE1? MoLyNE42KGh? MmDkZoxw[2u|IFnHSk1KUS2rbnT1Z4VlKFOWUzDtVm5CKGW6cILld5Nqd28EoB?= M4D4[lI1ODV3NUKw
THP-1  MVzGeY5kfGmxbjDBd5NigQ>? MWCxNOKh|ryP NYDpPWg5OcLiaB?= MoHsZYJwdGm|aHXzJJRp\SCnZn\lZ5Qhd2ZicljTVFI4KG:wIGPSMWEhdVKQQR?= NYq1S3ZPOjN7M{mzPVg>
A549 NI\3WoNHfW6ldHnvckBCe3OjeR?= NYfZd5VSOcLizszN MoHpOFghcA>? MYTlcohidmOnczD0bIUhfXBvcnXneYxifGmxbjDv[kBK|rqEIHHu[EB{fWK|ZYH1[Y51KGSnY4LlZZNmKGmwIFLhfEBmgHC{ZYPzbY9vKGmwZIXj[YQh[nliY3;tZolv\WRic4TpcZVt[XSrb36= NV\TbmttOjN7MECwPFA>
A549 M4LuNmFxd3C2b4Ppd{BCe3OjeR?= NVvXbpk{OcLizszN M4HzVVQ5KGh? M1\B[JJm\HWlZYOgeIhmKGOnbHyg[IVifGhiaX7keYNm\CCkeTDjc41jcW6nZDDzeIlufWyjdHnvci=> NVH5Z2xZOjN7MECwPFA>
NCI-N87 Ml76S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTXO25COTBxMkCvN|Ah|ryP NXy0NmlLPi9{NDDo MULzeZBxemW|c3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> MXGyN|g1PjV2NR?=
AGS NGP0R|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITDO4gyOC9{MD:zNEDPxE1? NF\ENGM3NzJ2IHi= Mljod5VxeHKnc4Pld{Bk\WyuII\pZYJqdGm2eTDzbYdvcW[rY3HueIx6 NWnX[3hlOjN6NE[1OFU>
MGC80-3 M4HUR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvLenAyOC9{MD:zNEDPxE1? NVXOWop6Pi9{NDDo MWrzeZBxemW|c3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> M4\ERlI{QDR4NUS1
HGC-27  M3TsZmZ2dmO2aX;uJGF{e2G7 M3:3S|cvPS9zNT:zNEDPxE1? MlrBOkBp NV;BUZU5cW6mdXPld{B1cGViZHXwbI9{eGixconsZZRqd25iYX7kJJVxNXKnZ4XsZZRqd25ib3[gTe67Ss7z MWeyN|g1PjV2NR?=
MGC80-3 NV;aPHFMTnWwY4Tpc44hSXO|YYm= MWS3MlUwOTVxM{Cg{txO MYC2JIg> M1TReolv\HWlZYOgeIhmKGSncHjvd5Bpd3K7bHH0bY9vKGGwZDD1dE1z\We3bHH0bY9vKG:oIFpOvmLPuQ>? M3[wdFI{QDR4NUS1
HGC-27  NUPLVodRSXCxcITvd4l{KEG|c3H5 NEjkcpA4NjVxMUWvN|Ah|ryP M3z5e|YhcA>? M36yNYlv\HWlZYOgZZBweHSxc3nzJIlvKGFidHnt[U0h[W6mIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NIjJWpczOzh2NkW0OS=>
HBE  NFnBTnFHfW6ldHnvckBCe3OjeR?= NGr6cVAyOM7:TR?= MUKzbC=> MWnhZo9tcXOqZYOgeIhmKGmwY4LlZZNmeyCxZjDJUE03KGW6cILld5Nqd25iaX7keYNm\CCkeTDDV2U> Mmm1NlM5OjRyOEm=
HepG2 M1XKc2Z2dmO2aX;uJGF{e2G7 MoXoNE4{NzFxMzFOwG0> M3HWU|Q5KGh? MWfy[YR2[2W|IFnMOk1qdmS3Y3XkJHBQVjFiZYjwdoV{e2mxbh?= NIm5S|czOzd7MUizNy=>
THP-1 NYXVWGlPTnWwY4Tpc44hSXO|YYm= M2PxeVUhyrWP M3ezO|EhcA>? NFHBXIhFVVOR NFfMcW5qdmirYnn0d{BOXEJvaX7keYNm\CCQRt86RkBi[3SrdnH0bY9v M2HzZ|I{PjN2MkG4
THP-1  M1fySGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{DTS|UhyrWP NWrBS|ZmPC96IHS= MkTpSG1UVw>? M3PzfJJm\HWlZYOgeIhmKH[rYXLpcIl1gSCxZjDpcpRz[WOnbHz1cIFzyqCPVFK= MWKyN|Y{PDJzOB?=
MDM Ml;FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDTOUDDvU1? Mo[5OE85KGR? M{DGcGROW09? MYny[YR2[2W|IITo[UB3cWGkaXzpeJkhd2ZiaX70doFk\WyudXzhduKhVVSE NEO2NZAzOzZ|NEKxPC=>
AM M4G4[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfj[5A2KML3TR?= NUC1e2VEPC96IHS= NILtWlRFVVOR NWDxXmpqemWmdXPld{B1cGVidnnhZoltcXS7IH;mJIlvfHKjY3XscJVt[XMEoF3URi=> M4L1WFI{PjN2MkG4
RAW 264  NV;PPYV4TnWwY4Tpc44hSXO|YYm= MlrnNE4zNTViwsXN MYezNE83OC97MDDtbY4> MWLpcohq[mm2czD0bIUheGixc4DoZZRie2ViYXP0bZZqfHlib3[gVHRROUMEoB?= NUDLNnh2OjN3N{izNFI>
HUVEC MnXBSpVv[3Srb36gRZN{[Xl? MUSxNOKh|ryP NUXIUo9nOC53IHi= MmX5SG1UVw>? M1\NW4NwfW62ZYLhZ5RmeyC2aHWgcI9{eyCxZjDUbYUzKG2UTlG= MlW3NlM2PjN4M{K=
HT29 MmC3SpVv[3Srb36gRZN{[Xl? NHTBWoYyOC9|MD:xNFAh|ryP NHvi[VcyKGh? M1W1ZYlvcGmkaYTld{Bjd3SqIGTXSWFMNWmwZIXj[YQheDFyMDDwdo9k\XO|aX7n NXXnZ2RMOjN3MkexOVQ>
HT29 MmLNSpVv[3Srb36gRZN{[Xl? NFzGOGsyOC9|MD:xNFAh|ryP MYixJIg> MkXRbY5pcWKrdIOgWG5HNWmwZIXj[YQheGixc4Doc5J6dGG2aX;uJIFv\CCmZXfyZYRifGmxbjDv[kBK|rqEzsG= M4\m[|I{PTJ5MUW0
MM.1S M33TWmFxd3C2b4Ppd{BCe3OjeR?= MY[zNEDDvU1? NULlPZpJOyCq Mor3bY5lfWOnczDNUUBk\WyuIHTlZZRpKGmwdn;seoV{KG6nY4Lvd4l{ MUWyN|UzPzF3NB?=
KMS-12-BM M1vGS2Fxd3C2b4Ppd{BCe3OjeR?= NWXlRmFtOzBiwsXN NEnzXYQ{KGh? M3znUIlv\HWlZYOgUW0h[2WubDDk[YF1cCCrbo\vcJZmeyCwZXPyc5Nqew>? M2PTPVI{PTJ5MUW0
BAFs NX;aT4lzTnWwY4Tpc44hSXO|YYm= NXf0b|NqOC53L{JCpO69VQ>? M1[3VlI1KGh? MYTpcohq[mm2czDUUmbPuS:GRWigbY5lfWOnZDDDXXAyQUFzwrD0doFve2O{aYD0dy=> MnrTNlM1QDV2NUe=
SP6.5 NWLSOVd4TnWwY4Tpc44hSXO|YYm= M1\kU|Uh|ryP M4\5R|IhcA>? NXXkbmJZ\GWlcnXhd4V{yqC2cnHud4xw[2G2aX;uJI9nKHB4NTDpckB1cGViboXjcIV2e8Li NYDCPYxuOjN2NEOwPFY>
VUP NIDXbYFHfW6ldHnvckBCe3OjeR?= Mn\0OUDPxE1? NGe3Z3kzKGh? MVrk[YNz\WG|ZYRCpJRz[W6|bH;jZZRqd25ib3[gdFY2KGmwIITo[UBvfWOuZYXzxsA> M4DE[VI{PDR|MEi2
OCM1 MUTGeY5kfGmxbjDBd5NigQ>? M1;YbVUh|ryP NVOzfXIyOiCq MWHk[YNz\WG|ZYRCpJRz[W6|bH;jZZRqd25ib3[gdFY2KGmwIITo[UBvfWOuZYXzxsA> MonyNlM1PDNyOE[=
OM431 M372UmZ2dmO2aX;uJGF{e2G7 M2WyT|Uh|ryP NULOd3B1OiCq NEjIXGJl\WO{ZXHz[ZPDqHS{YX7zcI9k[XSrb36gc4YheDZ3IHnuJJRp\SCwdXPs[ZV{yqB? M3;ofFI{PDR|MEi2
SP6.5 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\1O3NsOi53LUKwJO69VQ>? NYf3VG8zOjRiaNMg NFHqVpVKSzVyPUWg{txONCCneHjpZol1eyC|dILvcoch[W62aT3wdo9tcW[ncnH0bZZmKGWoZnXjeJMhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NWjZbohFOjN2NEOwPFY>
VUP NETzVoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4i5[lIvPS1{MDFOwG0> NFHKR5EzPCCqwrC= MX\JR|UxRTVizszNMEBmgGirYnn0d{B{fHKxbnegZY51cS2ycn;sbYZmemG2aY\lJIVn\mWldIOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MnPxNlM1PDNyOE[=
OCM1 MkXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVeyMlUuOjBizszN NHzpW3czPCCqwrC= MmPyTWM2OD13IN88UUwh\XiqaXLpeJMhe3S{b37nJIFvfGlvcILvcIln\XKjdHn2[UBm\m[nY4TzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M2fFNlI{PDR|MEi2
OM431 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\HNk42NTJyIN88US=> MUOyOEBpyqB? MX\JR|UxRTVizszNMEBmgGirYnn0d{B{fHKxbnegZY51cS2ycn;sbYZmemG2aY\lJIVn\mWldIOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NG[zW2UzOzR2M{C4Oi=>
SP6.5 MonaRZBweHSxc3nzJGF{e2G7 MUK1JO69VQ>? M3jPWFI1KGkEoB?= M4LLOYlv\HWlZYOgZZBweHSxc3nzxsA> MXWyN|Q1OzB6Nh?=
VUP MmfWRZBweHSxc3nzJGF{e2G7 NF;mZoY2KM7:TR?= M{DGO|I1KGkEoB?= MXHpcoR2[2W|IHHwc5B1d3Orc9Mg M3nnVlI{PDR|MEi2
OCM1 M4PpV2Fxd3C2b4Ppd{BCe3OjeR?= M3;HVlUh|ryP MViyOEBpyqB? NIHtSlFqdmS3Y3XzJIFxd3C2b4Ppd:Kh M371S|I{PDR|MEi2
OM431 NGPRPFNCeG:ydH;zbZMhSXO|YYm= MWi1JO69VQ>? NIHKfHQzPCCqwrC= NULld3YycW6mdXPld{BieG:ydH;zbZPDqA>? MlzwNlM1PDNyOE[=
SP6.5 NUj0Vm5RTnWwY4Tpc44hSXO|YYm= M4HMdlUh|ryP M{\KTVEzKGh? MV7y[YR2[2W|IITo[UBucWe{YYTpc47DqA>? M2TKTFI{PDR|MEi2
VUP NGmyVYZHfW6ldHnvckBCe3OjeR?= Mn6yOUDPxE1? NVvMSmVjOTJiaB?= NHG3PJhz\WS3Y3XzJJRp\SCvaXfyZZRqd28EoB?= M1[0[|I{PDR|MEi2
OCM1 MmHjSpVv[3Srb36gRZN{[Xl? MX:1JO69VQ>? NI\Kd2MyOiCq NET4TGNz\WS3Y3XzJJRp\SCvaXfyZZRqd28EoB?= MkHwNlM1PDNyOE[=
OM431 NILiPZZHfW6ldHnvckBCe3OjeR?= M13LclUh|ryP MkXhNVIhcA>? NHPsfGpz\WS3Y3XzJJRp\SCvaXfyZZRqd28EoB?= M{\4WlI{PDR|MEi2
HBL-1  NVLD[otuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUGyclNVO8LizszN MlHGNlQwPDhxN{KgbC=> MXvEUXNQ MVHzcI94eyClZXzsJIdzd3e2aDDtc4Rme3SueR?= M3\XUlI{PDRzN{Ow
RAW 264.7 M2i1SWZ2dmO2aX;uJGF{e2G7 Mn;jNk0yPSEQvF2= NVS5bYc2OSCq Mn;aSG1UVw>? MYjzeZBxemW|c3XzJJRp\SCjY4TpeoF1cW:wIH;mJGlMUyCoYX3pcJkhdWWvYnXydy=> MkPVNlM1PDF5M{C=
IL-1R  MUHGeY5kfGmxbjDBd5NigQ>? M1HSWVIuOTVizszN MojHNUBp M2DM[2ROW09? Mni0d5VxeHKnc4Pld{B1cGViYXP0bZZifGmxbjDv[kBKU0tiZnHtbYx6KG2nbXLldpM> M{npR|I{PDRzN{Ow
RAW 264.7 NHz6PGNHfW6ldHnvckBCe3OjeR?= NVXZTmIxOTVizszN M37TblEhcA>? MVjEUXNQ MX7zeZBxemW|c3XzJJRp\SCjY4TpeoF1cW:wIH;mJIFv\CCMTlu= NELxbm4zOzR2MUezNC=>
IL-1R  NGHubWxHfW6ldHnvckBCe3OjeR?= NV[1bmdyOTVizszN NF31eI4yKGh? NV3oOpRWTE2VTx?= MXfzeZBxemW|c3XzJJRp\SCjY4TpeoF1cW:wIH;mJIFv\CCMTlu= M4HjNFI{PDRzN{Ow
U2OS NFPkbWhHfW6ldHnvckBCe3OjeR?= MkjqNVUh|ryP NXGw[XdkOSCq Mn3GSG1UVw>? NFHqc3RxemW4ZX70d{B1cGViTGDTMUBweiCLTD2xMZN1cW23bHH0[YQh\m:{bXH0bY9vKG:oIFu2N{1xXWJiY3jhbY5{yqB? M4rvfVI{PDRzN{Ow
MT‐1  NEfNcItHfW6ldHnvckBCe3OjeR?= NVPIcnlvQMLiwsXt M4[4S|MhcA>? NEjvXFdl\WO{ZXHz[ZMhfGinIHzleoVteyCxZjDw5qCRW1SDVEOgZY5lKHEkgKC0SWJROQ>? M{DoNFI{Ojd6NEe5
MT‐2  MW\GeY5kfGmxbjDBd5NigQ>? NWLTV5FuQMLiwsXt NV\lc5lzOyCq MoG4[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZicPMAlHNVSVR|IHHu[EBx6oDSNFXCVFE> M373dFI{Ojd6NEe5
MT‐1  NHjOWZNHfW6ldHnvckBCe3OjeR?= M3zjfFjDqML3bR?= MY[zJIg> MYLk[YNz\WG|ZYOgeIhmKGyndnXsd{Bw\iC2aHWgdFY2KHO3YoXubZQhd2ZiTldihLDPwkMEoB?= NG\hRXQzOzJ5OES3PS=>
MT‐2  MkTFSpVv[3Srb36gRZN{[Xl? Ml22POKhyrWv NXOyXHI5OyCq M4nMVoRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIITo[UBxPjVic4XieY5qfCCxZjDOSwKBmM78QtMg Mo\LNlMzPzh2N{m=
MCF-7  NUnldWw3TnWwY4Tpc44hSXO|YYm= MnriNk42NTF3IN88US=> MX6wMlUhcA>? NWXjeYtNTE2VTx?= MoLSZ4F2e2W|IITo[UBoemGmdXHsJIxwe3Nib3[gZ4VtdCCjZHjld4lwdg>? M3jHWlI{ODl|MkK3
HaCaT  MknwSpVv[3Srb36gRZN{[Xl? NUK4dFM3PS5ywrFOwG0> MX:xxsBpyqB? M13NdoF1fGWwdXH0[ZMhfGinIGTDU2gucW6mdXPl[EBxem:mdXP0bY9vKG:oIFnMMVbDqA>? NHm5XFgzOzB2MUG2PC=>
A549  NEHtdlFHfW6ldHnvckBCe3OjeR?= M1Hs[|EhcA>? NFTHZXNqdmirYnn0d{BNXEFvaX7keYNm\CCVUD3BJI1TVkFicILv[JVkfGmxbtMgd4lodmmoaXPhcpRtgQ>? MV2yN|A{OTJzMx?=
OA chondrocytes  M1vCRmZ2dmO2aX;uJGF{e2G7 M{XQflExKM7:TR?= NXTt[|VMOSCq NHjlZZZjdG:la4OgeIhmKEGJRT3CV2EucW6mdXPl[EBo\W6nL4Dyc5RmcW5iZYjwdoV{e2mxbjDv[kBIWlB5ODDvdkBEV1hvMjCodFwxNjB3KR?= MVOyNlk5OjJ{OB?=
RAW264.7 MoG1SpVv[3Srb36gRZN{[Xl? MnjYNVUh|ryP M4fQPFE2NTF{MDDtbY4> NF\zUWhjdG:la4OgeIhmKHC{b3T1Z5Rqd25ib3[gUm8tKFCJRUKsJIFv\CCWTl[t{tE> NHjxdJMzOjd2NUWyNy=>
RAW264.7 M{jRXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{D2blUuOzBizszN NHe2[nYzPCCq MVjpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NIXzSZEzOjd2NUWyNy=>
HBL6 MWjBdI9xfG:|aYOgRZN{[Xl? MmDBNE42NzVxMkWg{txO NXnjXZJyPi9{NDDo NF3CPW1l\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZY5lKGynZXHkd{B1dyCjcH;weI9{cXNiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XMEoB?= NYrDZ5RZOjJyN{S4NlA>
HT29  NYixXGFFTnWwY4Tpc44hSXO|YYm= NY\Xd3NHOS1zMDFOwG0> MV:xNOKhcA>? NEPpd|ZqdmO{ZXHz[ZMhUE9vMTDtVm5CKGGwZDDwdo91\WmwIHX4dJJme3Orb36= MX:yNVYzODl4NB?=
Ca9–22 MXfBdI9xfG:|aYOgRZN{[Xl? MUSxNOKh|ryPwrC= NFH3eZUyKGh? M{HzToNwdXCuZYTlcJkhcW6qaXLpeJMhSUyDLWDEWE1qdmS3Y3XkJIFxd3C2b4Ppdy=> MYmyNVE{QDR6MB?=
Ca9–22 NHHrS3JHfW6ldHnvckBCe3OjeR?= MV2xNOKh|ryPwrC= Mmq3NUBp MoToZ49ueGyndHXsfUBi[nKxZ3H0[ZMhfGinIFHMRU1RTFRvaX7keYNm\CCMTlugZYN1cX[jdHnvci=> Mm\5NlEyOzh2OEC=
A-549 NUHLbW9GT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDSVVk{OTEEoN88UeKh MWWyOE81QCCq NEfKclJqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? NGj1W4YzODh4NkC0Ny=>
AP MVnGeY5kfGmxbjDBd5NigQ>? MkfDOU8yOCEQvF2= NGW5bHA1QCCq NYXM[HQ3\G:5boLl[5Vt[XSnczD0bIUhSkGGIIDyc5RmcW5ibHX2[Ywh[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NY\1VnpqOjB3OU[2OFU>
AQ1 MVPGeY5kfGmxbjDBd5NigQ>? NVjrV3ZTPS9zMDFOwG0> NFP3TVc1QCCq NIfCOXBld3ewcnXneYxifGW|IITo[UBDSURicILveIVqdiCuZY\lcEBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MYSyNFU6PjZ2NR?=
AP M2Dab2Z2dmO2aX;uJGF{e2G7 MWmyNEDPxE1? NXG3[IUzPC96IHi= NGj1Rphld3ewcnXneYxifGW|IITo[UBDSURicILveIVqdiCuZY\lcEBiKHSrbXWt[IVx\W6mZX70JI1idm6nch?= NVvUdIR7OjB3OU[2OFU>
AQ1 NVK2fpZ1TnWwY4Tpc44hSXO|YYm= NW\oOlc6OjBizszN M3;XdFQwQCCq MnrY[I94dnKnZ4XsZZRmeyC2aHWgRmFFKHC{b4TlbY4hdGW4ZXygZUB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? NX\zZ|VpOjB3OU[2OFU>
THP-1 MUnGeY5kfGmxbjDBd5NigQ>? NHjPZmY2yqEQvF5CpC=> NHPXXJUxNjViaB?= NUPPU5Jq[XS2ZX71ZZRmeyC2aHWgUHBUNWmwZIXj[YQheC2LzsrC{tEheHKxdHXpckBjgSB5MjW= MkLUNlA{ODl5MUi=
K562 NX3Q[mpXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzkdWYzNTNyIN88US=> MmOxNlQhcA>? MkjiTWM2OD16IN88UUxqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NGjoS4gyQTZ2NkiwOy=>
Jurket NIT1SZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV[yMVMxKM7:TR?= MnfuNlQhcA>? NF:3eppKSzVyPUeuNUDPxE1uIHnubIljcXS|IHPlcIwh\3Kxd4ToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MlLnNVk3PDZ6MEe=
U937 NY\wNYFqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrST|I5Oi1|MDFOwG0> NGDiOGgzPCCq MUjJR|UxRTFyLkWg{txONCCrbnjpZol1eyClZXzsJIdzd3e2aDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NYPrT|VOOTl4NE[4NFc>
PBMC NHfm[nJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYqyMVMxKM7:TR?= NYS2VYp2OjRiaB?= NE\0No1KSzVyPUSwMlIh|ryPLDDpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MlXoNVk3PDZ6MEe=
K562 MnXJRZBweHSxc3nzJGF{e2G7 MlO0Nk0zOMLizszNxsA> MVOyOEBp NXHCTJlXcW6mdXPld{BiKGSxc3Wt[IVx\W6mZX70JIFxd3C2b4Ppdy=> MWSxPVY1PjhyNx?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP (see the Experimental section). The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP. The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

31332209 23441730
Growth inhibition assay
Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

31332209

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[2]
- 合并
  • Cell lines: NCI-H1703 细胞
  • Concentrations: ~8 μM
  • Incubation Time: 12 小时
  • Method: 细胞在96孔板中转染siRNA,然后在完全NSCLC 培养基中培养72小时,再使用BAY 11-7082 处理12小时。细胞与[3H]胸甘温育3小时。使用自动细胞收集器将细胞收集到过滤器上,然后通过β-闪烁计数法测量放射性。
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 41 mg/mL (197.82 mM)
Water Insoluble
Ethanol ''10 mg/mL
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+corn oil
8mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 207.25
化学式

C10H9NO2S

CAS号 19542-67-7
储存条件 粉状
溶于溶剂
别名 BAY 11-7821

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

IκB/IKK Signaling Pathway Map

IκB/IKK Inhibitors with Unique Features

相关IκB/IKK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID