BAY 11-7082

For research use only. Not for use in humans.

目录号:S2913 别名: BAY 11-7821

BAY 11-7082 Chemical Structure

CAS No. 19542-67-7

BAY 11-7082 (BAY 11-7821)是一种 NF-κB 抑制剂,抑制TNFα诱导的 IκBα 磷酸化,在肿瘤细胞中IC50为10 μM。BAY 11-7082 可抑制 ubiquitin-specific protease USP7USP21,对应的IC50值分别为0.19 μM和0.96 μM。BAY 11-7082 在胃癌细胞中可诱导凋亡和S期阻滞。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 988.44 现货
RMB 738.58 现货
RMB 2223.41 现货
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客户使用Selleck生产的BAY 11-7082发表文献226篇:

产品安全说明书

IκB/IKK抑制剂选择性比较

生物活性

产品描述 BAY 11-7082 (BAY 11-7821)是一种 NF-κB 抑制剂,抑制TNFα诱导的 IκBα 磷酸化,在肿瘤细胞中IC50为10 μM。BAY 11-7082 可抑制 ubiquitin-specific protease USP7USP21,对应的IC50值分别为0.19 μM和0.96 μM。BAY 11-7082 在胃癌细胞中可诱导凋亡和S期阻滞。
靶点
E2-conjugating enzymes [6]
(Cell-free assay)
USP7 [7]
(Cell-free assay)
USP21 [7]
(Cell-free assay)
USP6 [7]
(Cell-free assay)
IκBα phosphorylation [1]
(Tumor cells)
0.19 μM 0.96 μM 1.7 μM 10 μM
体外研究

BAY 11-7082完全且特异性地废除 NF-κB DNA结合, 下调NF-κB-诱导的细胞因子IL-6,且诱导细胞凋亡。[1] BAY 11-7082 (< 8 μM) 有效抑制原有的和TNFα 刺激的NF-κB荧光素酶活性,这种作用存在剂量依赖性。BAY 11-7082 (8 μM) 强抑制NCI-H1703 细胞增殖。 [2] Bay 11-7082 (5 μM)作用于感染HTLV-I的T细胞系,快速且有效的降低NF-κB的DNA结合力,且下调抗凋亡基因Bcl-x(L)的表达,而对另一种转录因子AP-1与DNA结合没有作用效果。Bay 11-7082诱导原代ATL细胞发生凋亡比正常的外周血单核细胞更明显,而诱导的这些细胞凋亡也与NF-κB活性的下调相关。Bay 11-7082 (5 μM) 选择性诱导感染HTLV-I的T细胞系发生细胞凋亡,且下调 cyclin D1, cyclin D2, 和 Bcl-xL的表达。 [3] BAY 11-7082 (100 μM)作用于小鼠海马脑片区,抑制NMDA引起的p65发生 核易位,也抑制NMDA诱导的NF-κB结合力的增强。BAY 11-7082抑制NMDA毒性发生在海马脑片区的CA1区,20 μM时,对40%神经有保护作用,100 μM时,对70%神经有保护作用。[4]作用于脂肪组织,实验中所有浓度的BAY 11-7082都显著抑制 NF-κB p65 DNA结合活性,而作用于骨骼肌,只有50 μM 和 100 μM 的BAY 11-7082显著抑制NF-κB p65 DNA结合活性。BAY 11-7082 (100 μM) 作用于人体脂肪组织和骨骼肌,降低IKK-β蛋白。BAY 11-7082 (100 μM) 显著降低TNF-α从动物脂肪组织中释放,而 IL-6 和 IL-8 的释放显著抑制实验中BAY 11-7082的所有浓度。 BAY 11-7082 (50 μM)作用于骨骼肌,显著降低 TNF-α, IL-6, 和IL-8 的释放。[5] 此外,BAY 11-7082还被发现抑制了 E2-conjugating酶和E3 ligase酶的活性,从而诱导了B-淋巴癌细胞和T淋巴癌细胞的死亡。 [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa NYG1eYh3TnWwY4Tpc44hSXO|YYm= MnzBNVDDqM7:TR?= NFn0VJIyNjViaB?= MlLLZYJwdGm|aHXzJGJRSSCrbnT1Z4VlKHWyIILl[5Vt[XSrb36gc4YhTk5iYX7kJG1OWC17wrC= M{nz[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{m3OFM4Lz5{NUe5O|Q{PzxxYU6=
SiHa  NEXWNHpHfW6ldHnvckBCe3OjeR?= MVixNOKh|ryP MYWxMlUhcA>? M2fYPIFjd2yrc3jld{BDWEFiaX7keYNm\CC3cDDy[Yd2dGG2aX;uJI9nKE[QIHHu[EBOVVBvOdMg M4n1WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{m3OFM4Lz5{NUe5O|Q{PzxxYU6=
ARPE-19 M2jLPGZ2dmO2aX;uJGF{e2G7 NEDlXGgyKM7:TR?= M2jBXFAvPSCq M1TYVJN2eHC{ZYPz[ZMhXEdvaX7keYNm\MLiSVytPOKheHKxbX;0[ZIh[WO2aY\heIlwdg>? NYLSNpd4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1PVMxOjlpPkK1OVk{ODJ7PD;hQi=>
HCT116 M1XsdGZ2dmO2aX;uJGF{e2G7 MmHlOUDPxE1? NU\QPXlQOiCq NVOzU5drTE2VTx?= NYnI[og2[XS2ZX71ZZRmeyC|aXz5cYFzcW5vaX7keYNm\CCmb4fudoVofWyjdHnvckBw\iCleXPsbY4hTDF? NWC3NmkxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0O|k4OjNpPkK1OFc6PzJ|PD;hQi=>
HMECs NYPHbXNITnWwY4Tpc44hSXO|YYm= MoriOeKh|ryP MnXtNkBp MWjhZo9tcXOqZYOgWG5HNc7zLXnu[JVk\WRiVlPBUU0yKGW6cILld5Nqd28EoB?= Mn7uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzOUOxNVYoRjJ3MUmzNVE3RC:jPh?=
A549  M1vD[GZ2dmO2aX;uJGF{e2G7 NIjwfVkyOOLCidM1US=> NEHxbGoyOiCq NF:1[JV{fXCycnXzd4V{KES4bD2zJIlv\HWlZXSgZYN1cX[jdHnvckBw\iCyNkW= MkDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNU[4NFAoRjJ3MUW2PFAxRC:jPh?=
RAW 264.7 NI\3[nNHfW6ldHnvckBCe3OjeR?= M37kV|XDqM7:TR?= MnT0NUBp MlHQbY5pcWKrdIOgWG5HNc7zIHHu[EBKVC1zMjDwOFAheHKxZIXjeIlwdg>? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTBzOUW2O{c,OjVyMUm1Olc9N2F-
macrophages NYjJTI1QTnWwY4Tpc44hSXO|YYm= M1K1dFUhyrWP NUG5[3g3OyCq MkDZdIFzfGmjbHz5JIJtd2OtczDZVGZUNWmwZIXj[YQh\XiycnXzd4lwdiCxZjDpUm9UKGGwZDDDU3guOsLi NUDib21[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5Olc5QThpPkK0PVY4QDl6PD;hQi=>
HUVECs NYrteVFJTnWwY4Tpc44hSXO|YYm= NIjyfVQ{NTNyIN88US=> MV2xJIg> M1PPTJJm\HWlZYOgeIhmKGW6cILld5Nqd25ib3[gcYlTNTF2NnGgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MkPVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NkO5OlUoRjJ2OE[zPVY2RC:jPh?=
HeLa  MmLZSpVv[3Srb36gRZN{[Xl? MV:1xsDPxE1? MoHuNlQhcA>? Mn\PSG1UVw>? MknBdoVlfWOnczD0bIUh[WO2aY\peJkhd2ZiVF7GMe6yKHC{b33veIVzyqB? MmfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4NUe3PFMoRjJ2NkW3O|g{RC:jPh?=
A549 NX\GNIdITnWwY4Tpc44hSXO|YYm= NIq2[WgyOCEQvF2= NH;3VGgy6oDLaB?= NF24c3dqdmirYnn0d{B1cGViaX7jdoVie2Vib3[gdIhwe3Cqbz3J{tpD|rFiaX6gVGEyODNvaX7m[YN1\WRiY4XseJVz\XQEoB?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZzMkS4PEc,OjR4MUK0PFg9N2F-
HUVEC MV;GeY5kfGmxbjDBd5NigQ>? MVWyNEDDvU1? MX2wMlUhcA>? MnH3SG1UVw>? MkXPdJJmfmWwdIOgeIhmKGmwZIXjeIlwdiCxZjDFRW0h\XiycnXzd4lwdg>? MoP4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3NUGyNFkoRjJ2NUWxNlA6RC:jPh?=
A549RT-eto NUnlUVhKSXCxcITvd4l{KEG|c3H5 NH24UmcyOMLizszN NVTSZlI{OjRiaB?= MlPPSG1UVw>? MkLiZYNk\WyncnH0[ZMhTkWUTz3t[YRq[XSnZDDhdI9xfG:|aYO= MoLoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3M{WwPFMoRjJ2NUO1NFg{RC:jPh?=
THP-1  MmXTSpVv[3Srb36gRZN{[Xl? M3jJeFAvOS9zwrFOwG0> Mn3HNE42KGh? MWDhZpJw\2G2ZYOgWG5HNc7zIIPlZ5JmfGmxbjDhd{B4\WyuIHHzJJRp\SCrbnPy[YF{\WRic3XjdoV1cW:wIH;mJGlNNTZiYX7kJGlNNTIQsh?= NHHmXnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO3PFU{Pid-MkSzO|g2OzZ:L3G+
SKCXCR2  MlK2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWOyJOK2VQ>? M13Re|Q5KGh? NWTHSXhm\GWlcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdiC|aXfubYZq[2GwdHz5 MkTIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|N{[3OFcoRjJ2M{e2O|Q4RC:jPh?=
SKCXCR2  MVXGeY5kfGmxbjDBd5NigQ>? NVHpT|IyOiEEtV2= NWHOdVJkPDhiaB?= M3vjXoJtd2OtczD0bIUhS1iFTEGtbY5lfWOnZDDj[YxtKGmwdnHzbY9v M1\zSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{e2O|Q4Lz5{NEO3Olc1PzxxYU6=
OVCXCR2 NGK4ZnJHfW6ldHnvckBCe3OjeR?= NH:5Z4gzKML3TR?= NW\0OmVZPDhiaB?= NVzSdYFV[myxY3vzJJRp\SCFWFPMNU1qdmS3Y3XkJINmdGxiaX72ZZNqd25? NHKxVWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO3Olc1Pyd-MkSzO|Y4PDd:L3G+
DSCs  NF75R2NHfW6ldHnvckBCe3OjeR?= NXzkSpRQOi53IN88US=> MWewMlUhcA>? NIK1PIxz\X[ncoPld{B1cGViZX7oZY5k\W2nboSgc4YhS0OOMj;DR3IzKGW6cILld5Nqd25ib3[gSHNEeyCrbnT1Z4VlKGK7IFnMMVM{ Mke0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NESyOFAoRjJ2M{S0NlQxRC:jPh?=
WPs MXzGeY5kfGmxbjDBd5NigQ>? M1v6UlI2KM7:TR?= NVjqXIg5PSCvaX6= NVzwc4RLe3WycILld5NmeyCDVGCgZY5lKH[ZRjDz[YNz\XSrb36= NYDPUlZNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzN|EzODdpPkK0N|MyOjB5PD;hQi=>
A549RT-eto NVzoOnFOSXCxcITvd4l{KEG|c3H5 NHXu[G0yOMLizszN NEjVd24zPCCq MYjhZ4NmdGW{YYTld{BHOTRiZYj0doFkfC2vZXTpZZRm\CCjcH;weI9{cXNiY3;tZolv\WRidILlZZRu\W62IIfpeIghTjF2 MnTsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkC3NlUoRjJ2MkKwO|I2RC:jPh?=
A549RT-eto NIHhS4FHfW6ldHnvckBCe3OjeR?= NYO4XXdROTEEoN88US=> MXuyOEBp MnPQ[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDOSk3PwkJiYX7kJHAu\3EEoB?= MmLKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkC3NlUoRjJ2MkKwO|I2RC:jPh?=
FaDu NICyeJBHfW6ldHnvckBCe3OjeR?= M{XRSVIhcA>? M3ftTYlvcGmkaYTzJJA3PSCneIDy[ZN{cW:wIHHu[EBjdG:la4OgWG5H|rFvaX7keYNm\CCWV1nTWEBmgHC{ZYPzbY9v MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{ME[yNkc,OjR{MkC2NlI9N2F-
IVD  MoDySpVv[3Srb36gRZN{[Xl? NFHEXpoyOMLizszN M33WR|Mh\A>? M4DteJJmfmW{c3XzJHRPTi4QsfMAl41m\GmjdHXkJJN2eHC{ZYPzbY9vKG:oIITo[UBlcXOlIH3heJJqgCCvYXPyc41wdGWldXzld{Bi\2e{ZXPhckBidmRiY3;scIFo\W5iSVm= NIjzSoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3OlgxQCd-MkSxO|Y5ODh:L3G+
IVD  NW\3emQyTnWwY4Tpc44hSXO|YYm= MWCxNOKh|ryP M2TmbFMh\A>? NUD2UlNt[WK{b3fheIV{KFSQRj5OtgKBm2mwZIXj[YQhfXBvcnXneYxifGmxbjDv[kBCTEGPVGOtOEBidmRiQVTBUXRUNTYEoB?= NWfmR4lORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxO|Y5ODhpPkK0NVc3QDB6PD;hQi=>
iNKT M1GwUWZ2dmO2aX;uJGF{e2G7 M3uxeFExNzFyMDFOwG0> M2TqelAvPSCq M1HibIlvcGmkaYTzJJRp\SCrbnT1Z5Rqd25ib3[gRVJCWiCvUl7BJIFv\CCxdHjldkBn[WO2b4K= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF{NES1N{c,OjRzMkS0OVM9N2F-
PC-3 NX;penh6TnWwY4Tpc44hSXO|YYm= NGO0eHEzNjVxNT:xNEDPxE1? NXfmcHZyOC53IHi= MV7icI9kc3NiSVfGMWlKNWmwZIXj[YQhW1SVIH3SUmEh\XiycnXzd4lwdsLi NFr6PYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC1OVUzOCd-MkSwOVU2OjB:L3G+
THP-1  Moe1SpVv[3Srb36gRZN{[Xl? MknKNVDDqM7:TR?= M1:0UlHDqGh? NWHy[GUx[WKxbHnzbIV{KHSqZTDl[oZm[3Rib3[gdmhUWDJ5IH;uJHNTNUFibWLORS=> Ml\KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7M{mzPVgoRjJ|OUO5N|k5RC:jPh?=
A549 M3jSdGZ2dmO2aX;uJGF{e2G7 Mn3VNeKh|ryP NFTBW5A1QCCq M37G[oVvcGGwY3XzJJRp\SC3cD3y[Yd2dGG2aX;uJI9nKEoQulKgZY5lKHO3YoPldZVmdnRiZHXjdoVie2ViaX6gRoF5KGW6cILld5Nqd25iaX7keYNm\CCkeTDjc41jcW6nZDDzeIlufWyjdHnvci=> NYnjOoNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFAxQDBpPkKzPVAxODhyPD;hQi=>
A549 MofsRZBweHSxc3nzJGF{e2G7 MlvqNeKh|ryP MVO0PEBp NYHmUlBDemWmdXPld{B1cGViY3XscEBl\WG2aDDpcoR2[2WmIHL5JINwdWKrbnXkJJN1cW23bHH0bY9v NWrYTmxwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFAxQDBpPkKzPVAxODhyPD;hQi=>
NCI-N87 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vvPFExNzJyL{OwJO69VQ>? NUHrZ3lQPi9{NDDo MmXUd5VxeHKnc4Pld{Bk\WyuII\pZYJqdGm2eTDzbYdvcW[rY3HueIx6 M1nWS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OES2OVQ2Lz5{M{i0OlU1PTxxYU6=
AGS NFfYZXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUOxNE8zOC9|MDFOwG0> MVS2M|I1KGh? M1GxXpN2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgd4lodmmoaXPhcpRtgQ>? MnryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NE[1OFUoRjJ|OES2OVQ2RC:jPh?=
MGC80-3 NGf0XYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonDNVAwOjBxM{Cg{txO NIr0U4g3NzJ2IHi= M3z5eJN2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgd4lodmmoaXPhcpRtgQ>? NH\WdW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0OlU1PSd-MkO4OFY2PDV:L3G+
HGC-27  NIrKfXJHfW6ldHnvckBCe3OjeR?= NWX4dItoPy53L{G1M|MxKM7:TR?= M4q4blYhcA>? NX\NcnRQcW6mdXPld{B1cGViZHXwbI9{eGixconsZZRqd25iYX7kJJVxNXKnZ4XsZZRqd25ib3[gTe67Ss7z M1zjVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OES2OVQ2Lz5{M{i0OlU1PTxxYU6=
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HGC-27  NIq1dFBCeG:ydH;zbZMhSXO|YYm= NVf1[|FtPy53L{G1M|MxKM7:TR?= MVW2JIg> NUHlVJJycW6mdXPld{BieG:ydH;zbZMhcW5iYTD0bY1mNSCjbnSg[I9{\S2mZYDlcoRmdnRibXHucoVz M4\RRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OES2OVQ2Lz5{M{i0OlU1PTxxYU6=
HBE  M2HSN2Z2dmO2aX;uJGF{e2G7 NIjHblkyOM7:TR?= NGD5c4g{cA>? M1m3U4Fjd2yrc3jld{B1cGViaX7jdoVie2W|IH;mJGlNNTZiZYjwdoV{e2mxbjDpcoR2[2WmIHL5JGNUTQ>? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NEC4PUc,OjN6MkSwPFk9N2F-
HepG2 MWLGeY5kfGmxbjDBd5NigQ>? MlqxNE4{NzFxMzFOwG0> MXO0PEBp M1XU[JJm\HWlZYOgTWw3NWmwZIXj[YQhWE:QMTDlfJBz\XO|aX;u MkPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUG4N|MoRjJ|N{mxPFM{RC:jPh?=
THP-1 MmHDSpVv[3Srb36gRZN{[Xl? NXznRnYzPSEEtV2= MVuxJIg> MX3EUXNQ NXPUdldscW6qaXLpeJMhVVSELXnu[JVk\WRiTldOvmIh[WO2aY\heIlwdg>? NX7mV5lURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2N|QzOThpPkKzOlM1OjF6PD;hQi=>
THP-1  MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEnpZY82KML3TR?= NVrWSpVmPC96IHS= MoPoSG1UVw>? M3Hpc5Jm\HWlZYOgeIhmKH[rYXLpcIl1gSCxZjDpcpRz[WOnbHz1cIFzyqCPVFK= M{HPd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkO0NlE5Lz5{M{[zOFIyQDxxYU6=
MDM NWG4c3JiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HhWFUhyrWP M4TQ[lQwQCCm MVjEUXNQ NGnjc5Rz\WS3Y3XzJJRp\SC4aXHibYxqfHlib3[gbY51emGlZXzseYxiesLiTWTC MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ|NEKxPEc,OjN4M{SyNVg9N2F-
AM NYi0Z3h6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF[1XlM2KML3TR?= MVS0M|gh\A>? MoC0SG1UVw>? NUm1ZZpSemWmdXPld{B1cGVidnnhZoltcXS7IH;mJIlvfHKjY3XscJVt[XMEoF3URi=> NF;rSoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[zOFIyQCd-MkO2N|QzOTh:L3G+
RAW 264  NXvvPHFWTnWwY4Tpc44hSXO|YYm= MWOwMlIuPSEEtV2= Mk\KN|AwPjBxOUCgcYlv M4DVc4lvcGmkaYTzJJRp\SCyaH;zdIhifGG|ZTDhZ5Rqfmm2eTDv[kBRXFBzQtMg M4\rdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUe4N|AzLz5{M{W3PFMxOjxxYU6=
HUVEC Mn21SpVv[3Srb36gRZN{[Xl? MVexNOKh|ryP MnXZNE42KGh? MYTEUXNQ MmHmZ492dnSncnHjeIV{KHSqZTDsc5N{KG:oIGTp[VIhdVKQQR?= NHPMTZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W2N|Y{Oid-MkO1OlM3OzJ:L3G+
HT29 MULGeY5kfGmxbjDBd5NigQ>? NUXtdot3OTBxM{CvNVAxKM7:TR?= NYLGc3NZOSCq NGHJT3hqdmirYnn0[ZMh[m:2aDDUW2VCUy2rbnT1Z4VlKHBzMECgdJJw[2W|c3nu[y=> NHjP[VI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WyO|E2PCd-MkO1NlcyPTR:L3G+
HT29 MmXSSpVv[3Srb36gRZN{[Xl? MnrpNVAwOzBxMUCwJO69VQ>? NYDX[|JHOSCq NVPrWpFvcW6qaXLpeJMhXE6ILXnu[JVk\WRicHjvd5Bpd3K7bHH0bY9vKGGwZDDk[Ydz[WSjdHnvckBw\iCLzsrC{tE> M{DZU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK3NVU1Lz5{M{WyO|E2PDxxYU6=
MM.1S MkDRRZBweHSxc3nzJGF{e2G7 MmraN|AhyrWP NYrIN2d2OyCq NYnMbZB{cW6mdXPld{BOVSClZXzsJIRm[XSqIHnueo9tfmW|IH7lZ5Jwe2m| NYrydGx[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NlcyPTRpPkKzOVI4OTV2PD;hQi=>
KMS-12-BM M{H5RmFxd3C2b4Ppd{BCe3OjeR?= NGDDd48{OCEEtV2= NU[4blJlOyCq MXjpcoR2[2W|IF3NJINmdGxiZHXheIghcW64b3z2[ZMhdmWlcn;zbZM> NV7zbm9IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NlcyPTRpPkKzOVI4OTV2PD;hQi=>
BAFs MWrGeY5kfGmxbjDBd5NigQ>? M1;xOlAvPS9zwrFOwG0> NEjHVZYzPCCq M{HzWIlvcGmkaYTzJHRPTs7zL1TFXEBqdmS3Y3XkJGN[WDF7QUJCpJRz[W6|Y4LpdJR{ MnPTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OEW0OVcoRjJ|NEi1OFU4RC:jPh?=
SP6.5 NILac5ZHfW6ldHnvckBCe3OjeR?= M4jaN|Uh|ryP M17Ee|IhcA>? MmHC[IVkemWjc3XzxsB1emGwc3zvZ4F1cW:wIH;mJJA3PSCrbjD0bIUhdnWlbHX1d:Kh M{OxfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
VUP M3TVUmZ2dmO2aX;uJGF{e2G7 M2W2NlUh|ryP NInLNIEzKGh? MY\k[YNz\WG|ZYRCpJRz[W6|bH;jZZRqd25ib3[gdFY2KGmwIITo[UBvfWOuZYXzxsA> NXvtfJI2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFMxQDZpPkKzOFQ{ODh4PD;hQi=>
OCM1 MV7GeY5kfGmxbjDBd5NigQ>? NXPkfFZxPSEQvF2= MkPHNkBp NUHmVYJv\GWlcnXhd4V{yqC2cnHud4xw[2G2aX;uJI9nKHB4NTDpckB1cGViboXjcIV2e8Li M4DOZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
OM431 NVPYfpZNTnWwY4Tpc44hSXO|YYm= MVK1JO69VQ>? Mn61NkBp M{DqO4Rm[3KnYYPld:KhfHKjboPsc4NifGmxbjDv[kBxPjViaX6geIhmKG63Y3zleZPDqA>? MmDGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEOwPFYoRjJ|NESzNFg3RC:jPh?=
SP6.5 NHLBWmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnz0Nk42NTJyIN88US=> NIjqcXIzPCCqwrC= M4XRb2lEPTB;NTFOwG0tKGW6aHnibZR{KHO2cn;u[{BidnSrLYDyc4xq\mW{YYTpeoUh\W[oZXP0d{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> M1rWelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
VUP NXLpfoR2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlS5Nk42NTJyIN88US=> MnK2NlQhcMLi M13qSGlEPTB;NTFOwG0tKGW6aHnibZR{KHO2cn;u[{BidnSrLYDyc4xq\mW{YYTpeoUh\W[oZXP0d{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MnW5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEOwPFYoRjJ|NESzNFg3RC:jPh?=
OCM1 NYHWRnBJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2rzNFIvPS1{MDFOwG0> MkHDNlQhcMLi MlHITWM2OD13IN88UUwh\XiqaXLpeJMhe3S{b37nJIFvfGlvcILvcIln\XKjdHn2[UBm\m[nY4TzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OM431 MlXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M13nOVIvPS1{MDFOwG0> M1XMPVI1KGkEoB?= M3n6SWlEPTB;NTFOwG0tKGW6aHnibZR{KHO2cn;u[{BidnSrLYDyc4xq\mW{YYTpeoUh\W[oZXP0d{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
SP6.5 NXroSHdNSXCxcITvd4l{KEG|c3H5 MYq1JO69VQ>? NWPOU2t3OjRiaNMg NGjCVYFqdmS3Y3XzJIFxd3C2b4Ppd:Kh M1\McVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
VUP MX3BdI9xfG:|aYOgRZN{[Xl? M1vk[lUh|ryP MWGyOEBpyqB? NVPlOXU6cW6mdXPld{BieG:ydH;zbZPDqA>? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OCM1 NFi5bVlCeG:ydH;zbZMhSXO|YYm= NWrTeG9KPSEQvF2= NHH1ZpUzPCCqwrC= M{XzNYlv\HWlZYOgZZBweHSxc3nzxsA> NXe5T2pYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFMxQDZpPkKzOFQ{ODh4PD;hQi=>
OM431 Mn7ORZBweHSxc3nzJGF{e2G7 NEjWb5M2KM7:TR?= NUPtPYd7OjRiaNMg NX\UdWhbcW6mdXPld{BieG:ydH;zbZPDqA>? MojyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEOwPFYoRjJ|NESzNFg3RC:jPh?=
SP6.5 NYrUToQ6TnWwY4Tpc44hSXO|YYm= MUO1JO69VQ>? MXixNkBp MXPy[YR2[2W|IITo[UBucWe{YYTpc47DqA>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
VUP M3flcWZ2dmO2aX;uJGF{e2G7 MUO1JO69VQ>? MnLaNVIhcA>? MmTzdoVlfWOnczD0bIUhdWmpcnH0bY9vyqB? NYjwZWNzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFMxQDZpPkKzOFQ{ODh4PD;hQi=>
OCM1 M4L4N2Z2dmO2aX;uJGF{e2G7 NFnwW5I2KM7:TR?= NFLNdlUyOiCq M2rKbpJm\HWlZYOgeIhmKG2rZ4LheIlwdsLi M2rycFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
OM431 MoHsSpVv[3Srb36gRZN{[Xl? NWD5[FJDPSEQvF2= MVmxNkBp NEfp[ldz\WS3Y3XzJJRp\SCvaXfyZZRqd28EoB?= M2nlclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
HBL-1  NI\ubnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWWzxsDPxE1? MWKyOE81QC95MjDo NXXuWWRDTE2VTx?= M4TJXZNtd3e|IHPlcIwh\3Kxd4ToJI1w\GW|dHz5 MlXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEG3N|AoRjJ|NESxO|MxRC:jPh?=
RAW 264.7 NUDrOpQ5TnWwY4Tpc44hSXO|YYm= NXTsS2JzOi1zNTFOwG0> NEmxb28yKGh? Ml3NSG1UVw>? M3LTc5N2eHC{ZYPz[ZMhfGinIHHjeIl3[XSrb36gc4YhUUuNIH\hcYltgSCvZX3i[ZJ{ M{f3U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESxO|MxLz5{M{S0NVc{ODxxYU6=
IL-1R  NEXmW45HfW6ldHnvckBCe3OjeR?= MmO4Nk0yPSEQvF2= MoS2NUBp NVjwVVNETE2VTx?= MYjzeZBxemW|c3XzJJRp\SCjY4TpeoF1cW:wIH;mJGlMUyCoYX3pcJkhdWWvYnXydy=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2MUezNEc,OjN2NEG3N|A9N2F-
RAW 264.7 M1SxdmZ2dmO2aX;uJGF{e2G7 MWqxOUDPxE1? NYnDUo1OOSCq M4PxfGROW09? MUDzeZBxemW|c3XzJJRp\SCjY4TpeoF1cW:wIH;mJIFv\CCMTlu= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2MUezNEc,OjN2NEG3N|A9N2F-
IL-1R  MoniSpVv[3Srb36gRZN{[Xl? M3;aO|E2KM7:TR?= NWTCWHdVOSCq NInuRmlFVVOR NXPDeJJYe3WycILld5NmeyC2aHWgZYN1cX[jdHnvckBw\iCjbnSgTm5M NH;yfmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0NVc{OCd-MkO0OFE4OzB:L3G+
U2OS MUPGeY5kfGmxbjDBd5NigQ>? NYD6O5NJOTVizszN MkDqNUBp NY[3THZQTE2VTx?= M4TaZZBz\X[nboTzJJRp\SCOUGOtJI9zKEmOLUGtd5RqdXWuYYTl[EBnd3KvYYTpc44hd2ZiS{[zMZBW[iClaHHpcpPDqA>? NUDkWWc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFE4OzBpPkKzOFQyPzNyPD;hQi=>
MT‐1  M2XwVWZ2dmO2aX;uJGF{e2G7 MV64xsDDvW1? MXuzJIg> MkSx[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZicPMAlHNVSVR|IHHu[EBx6oDSNFXCVFE> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ5OES3PUc,OjN{N{i0O|k9N2F-
MT‐2  NHLyeoVHfW6ldHnvckBCe3OjeR?= M4rBR|jDqML3bR?= NV;Be3Z1OyCq MnrG[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZicPMAlHNVSVR|IHHu[EBx6oDSNFXCVFE> NYjvUphRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO|g1PzlpPkKzNlc5PDd7PD;hQi=>
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP (see the Experimental section). The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP. The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

31332209 23441730
Growth inhibition assay
Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

31332209

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[2]

- 合并
  • Cell lines: NCI-H1703 细胞
  • Concentrations: ~8 μM
  • Incubation Time: 12 小时
  • Method:

    细胞在96孔板中转染siRNA,然后在完全NSCLC 培养基中培养72小时,再使用BAY 11-7082 处理12小时。细胞与[3H]胸甘温育3小时。使用自动细胞收集器将细胞收集到过滤器上,然后通过β-闪烁计数法测量放射性。


    (Only for Reference)

溶解度 (25°C)

体外 DMSO 41 mg/mL (197.82 mM)
Ethanol 10 mg/mL (48.25 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+corn oil
8mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 207.25
化学式

C10H9NO2S

CAS号 19542-67-7
储存条件 粉状
溶于溶剂
别名 BAY 11-7821

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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