BAY 11-7082

目录号:S2913 别名: BAY 11-7821

仅限科研使用

BAY 11-7082 (BAY 11-7821)是一种 NF-κB 抑制剂,抑制TNFα诱导的 IκBα 磷酸化,在肿瘤细胞中IC50为10 μM。BAY 11-7082 可抑制 ubiquitin-specific protease USP7USP21,对应的IC50值分别为0.19 μM和0.96 μM。BAY 11-7082 在胃癌细胞中可诱导凋亡和S期阻滞。

BAY 11-7082 Chemical Structure

CAS: 19542-67-7

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 988.44 现货
RMB 738.58 现货
RMB 2223.41 现货
大包装 有超大折扣

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客户使用Selleck生产的BAY 11-7082发表文献285篇:

产品安全说明书

IκB/IKK抑制剂选择性比较

相关IκB/IKK产品

生物活性

产品描述 BAY 11-7082 (BAY 11-7821)是一种 NF-κB 抑制剂,抑制TNFα诱导的 IκBα 磷酸化,在肿瘤细胞中IC50为10 μM。BAY 11-7082 可抑制 ubiquitin-specific protease USP7USP21,对应的IC50值分别为0.19 μM和0.96 μM。BAY 11-7082 在胃癌细胞中可诱导凋亡和S期阻滞。
靶点
E2-conjugating enzymes [6]
(Cell-free assay)
USP7 [7]
(Cell-free assay)
USP21 [7]
(Cell-free assay)
USP6 [7]
(Cell-free assay)
IκBα phosphorylation [1]
(Tumor cells)
0.19 μM 0.96 μM 1.7 μM 10 μM
体外研究

BAY 11-7082完全且特异性地废除 NF-κB DNA结合, 下调NF-κB-诱导的细胞因子IL-6,且诱导细胞凋亡。[1] BAY 11-7082 (< 8 μM) 有效抑制原有的和TNFα 刺激的NF-κB荧光素酶活性,这种作用存在剂量依赖性。BAY 11-7082 (8 μM) 强抑制NCI-H1703 细胞增殖。 [2] Bay 11-7082 (5 μM)作用于感染HTLV-I的T细胞系,快速且有效的降低NF-κB的DNA结合力,且下调抗凋亡基因Bcl-x(L)的表达,而对另一种转录因子AP-1与DNA结合没有作用效果。Bay 11-7082诱导原代ATL细胞发生凋亡比正常的外周血单核细胞更明显,而诱导的这些细胞凋亡也与NF-κB活性的下调相关。Bay 11-7082 (5 μM) 选择性诱导感染HTLV-I的T细胞系发生细胞凋亡,且下调 cyclin D1, cyclin D2, 和 Bcl-xL的表达。 [3] BAY 11-7082 (100 μM)作用于小鼠海马脑片区,抑制NMDA引起的p65发生 核易位,也抑制NMDA诱导的NF-κB结合力的增强。BAY 11-7082抑制NMDA毒性发生在海马脑片区的CA1区,20 μM时,对40%神经有保护作用,100 μM时,对70%神经有保护作用。[4]作用于脂肪组织,实验中所有浓度的BAY 11-7082都显著抑制 NF-κB p65 DNA结合活性,而作用于骨骼肌,只有50 μM 和 100 μM 的BAY 11-7082显著抑制NF-κB p65 DNA结合活性。BAY 11-7082 (100 μM) 作用于人体脂肪组织和骨骼肌,降低IKK-β蛋白。BAY 11-7082 (100 μM) 显著降低TNF-α从动物脂肪组织中释放,而 IL-6 和 IL-8 的释放显著抑制实验中BAY 11-7082的所有浓度。 BAY 11-7082 (50 μM)作用于骨骼肌,显著降低 TNF-α, IL-6, 和IL-8 的释放。[5] 此外,BAY 11-7082还被发现抑制了 E2-conjugating酶和E3 ligase酶的活性,从而诱导了B-淋巴癌细胞和T淋巴癌细胞的死亡。 [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa NEPIc4dHfW6ldHnvckBCe3OjeR?= MU[xNOKh|ryP MmnxNU42KGh? Mor3ZYJwdGm|aHXzJGJRSSCrbnT1Z4VlKHWyIILl[5Vt[XSrb36gc4YhTk5iYX7kJG1OWC17wrC= NHf6fYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe5O|Q{Pyd-MkW3PVc1Ozd:L3G+
SiHa  MknOSpVv[3Srb36gRZN{[Xl? MV:xNOKh|ryP MUOxMlUhcA>? M{LEVoFjd2yrc3jld{BDWEFiaX7keYNm\CC3cDDy[Yd2dGG2aX;uJI9nKE[QIHHu[EBOVVBvOdMg NH\OTFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe5O|Q{Pyd-MkW3PVc1Ozd:L3G+
ARPE-19 MYjGeY5kfGmxbjDBd5NigQ>? NHPy[IQyKM7:TR?= NFLvVXgxNjViaB?= MmXrd5VxeHKnc4Pld{BVTy2rbnT1Z4VlyqCLTD24xsBxem:vb4TldkBi[3SrdnH0bY9v NIXKZlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW5N|AzQSd-MkW1PVMxOjl:L3G+
HCT116 NEXXWGpHfW6ldHnvckBCe3OjeR?= M4OxcFUh|ryP NE[xeWQzKGh? MX3EUXNQ M2XSR4F1fGWwdXH0[ZMhe2mueX3hdolvNWmwZIXj[YQh\G:5boLl[5Vt[XSrb36gc4Yh[3mlbHnuJGQy MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR5OUeyN{c,OjV2N{m3NlM9N2F-
HMECs M3HzVGZ2dmO2aX;uJGF{e2G7 NGjnOm02yqEQvF2= MmnMNkBp MXXhZo9tcXOqZYOgWG5HNc7zLXnu[JVk\WRiVlPBUU0yKGW6cILld5Nqd28EoB?= NIfuSmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG5N|EyPid-MkWxPVMyOTZ:L3G+
A549  MVzGeY5kfGmxbjDBd5NigQ>? M2jISFEx6oDLwsXN MmHSNVIhcA>? MknZd5VxeHKnc4Pld{BFfmxvMzDpcoR2[2WmIHHjeIl3[XSrb36gc4YheDZ3 MnfIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNU[4NFAoRjJ3MUW2PFAxRC:jPh?=
RAW 264.7 NGfTXmZHfW6ldHnvckBCe3OjeR?= NWfBUmVuPcLizszN M4XKN|EhcA>? MkfnbY5pcWKrdIOgWG5HNc7zIHHu[EBKVC1zMjDwOFAheHKxZIXjeIlwdg>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTBzOUW2O{c,OjVyMUm1Olc9N2F-
macrophages MmrlSpVv[3Srb36gRZN{[Xl? MnjsOUDDvU1? MkjUN{Bp MUPwZZJ1cWGubImgZoxw[2u|IGnQSnMucW6mdXPl[EBmgHC{ZYPzbY9vKG:oIHnOU3Mh[W6mIFPPXE0zyqB? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4N{i5PEc,OjR7Nke4PVg9N2F-
HUVECs MnvTSpVv[3Srb36gRZN{[Xl? NYqzd5M6Oy1|MDFOwG0> MUmxJIg> NU\2PIlzemWmdXPld{B1cGViZYjwdoV{e2mxbjDv[kBucVJvMUS2ZUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NXLtZmF[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OlM6PjVpPkK0PFY{QTZ3PD;hQi=>
HeLa  MofiSpVv[3Srb36gRZN{[Xl? NGX1SnU2yqEQvF2= MYKyOEBp M3njXmROW09? NV3uRog3emWmdXPld{B1cGViYXP0bZZqfHlib3[gWG5HNc7zIIDyc41wfGW{wrC= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ3N{e4N{c,OjR4NUe3PFM9N2F-
A549 MXPGeY5kfGmxbjDBd5NigQ>? MoXZNVAh|ryP NFvVSZAy6oDLaB?= M3;1RYlvcGmkaYTzJJRp\SCrbnPy[YF{\SCxZjDwbI9{eGixLVpOvmLPuSCrbjDQRVExOy2rbn\lZ5Rm\CCldXz0eZJme8Li M33WVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkGyOFg5Lz5{NE[xNlQ5QDxxYU6=
HUVEC M3fCXGZ2dmO2aX;uJGF{e2G7 MnO4NlAhyrWP NFvWXpQxNjViaB?= Mk\MSG1UVw>? M4K5XpBz\X[nboTzJJRp\SCrbnT1Z5Rqd25ib3[gSWFOKGW6cILld5Nqd25? M2TnVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUWxNlA6Lz5{NEW1NVIxQTxxYU6=
A549RT-eto MUnBdI9xfG:|aYOgRZN{[Xl? NHq2UngyOMLizszN NInxV4IzPCCq MkfZSG1UVw>? NYXONopU[WOlZXzldoF1\XNiRlXSU{1u\WSrYYTl[EBieG:ydH;zbZM> NIDkTHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzOVA5Oyd-MkS1N|UxQDN:L3G+
THP-1  M{fJWmZ2dmO2aX;uJGF{e2G7 MYKwMlEwOcLizszN MY[wMlUhcA>? NWfob2Jq[WK{b3fheIV{KFSQRj5OtUB{\WO{ZYTpc44h[XNid3XscEBieyC2aHWgbY5kemWjc3XkJJNm[3KndHnvckBw\iCLTD22JIFv\CCLTD2x{tI> Ml3YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|N{i1N|YoRjJ2M{e4OVM3RC:jPh?=
SKCXCR2  MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1vEW|IhyrWP NXvtcYhuPDhiaB?= MVrk[YNz\WG|ZYOgZ4VtdCCycn;sbYZmemG2aX;uJJNq\26rZnnjZY51dHl? M1jTNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{e2O|Q4Lz5{NEO3Olc1PzxxYU6=
SKCXCR2  MYjGeY5kfGmxbjDBd5NigQ>? M4TzO|IhyrWP M{\2c|Q5KGh? MXficI9kc3NidHjlJGNZS0xzLXnu[JVk\WRiY3XscEBqdn[jc3nvci=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN5Nke0O{c,OjR|N{[3OFc9N2F-
OVCXCR2 MV;GeY5kfGmxbjDBd5NigQ>? NFzNbJozKML3TR?= NVHMR|B7PDhiaB?= NYXneXQ2[myxY3vzJJRp\SCFWFPMNU1qdmS3Y3XkJINmdGxiaX72ZZNqd25? Mnj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|N{[3OFcoRjJ2M{e2O|Q4RC:jPh?=
DSCs  NH\XU3ZHfW6ldHnvckBCe3OjeR?= Ml\CNk42KM7:TR?= NUPyXIx{OC53IHi= NFLqbphz\X[ncoPld{B1cGViZX7oZY5k\W2nboSgc4YhS0OOMj;DR3IzKGW6cILld5Nqd25ib3[gSHNEeyCrbnT1Z4VlKGK7IFnMMVM{ MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN2NEK0NEc,OjR|NESyOFA9N2F-
WPs M4XPTmZ2dmO2aX;uJGF{e2G7 MWWyOUDPxE1? MV61JI1qdg>? M{Hj[JN2eHC{ZYPz[ZMhSVSSIHHu[EB3X0Zic3XjdoV1cW:w MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN|MUKwO{c,OjR|M{GyNFc9N2F-
A549RT-eto MorlRZBweHSxc3nzJGF{e2G7 MXexNOKh|ryP MUGyOEBp MnLxZYNk\WyncnH0[ZMhTjF2IHX4eJJi[3RvbXXkbYF1\WRiYYDvdJRwe2m|IHPvcYJqdmWmIITy[YF1dWWwdDD3bZRpKEZzNB?= NYC2XYpSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlA4OjVpPkK0NlIxPzJ3PD;hQi=>
A549RT-eto NYXJUW5MTnWwY4Tpc44hSXO|YYm= M2PKSVExyqEQvF2= MWOyOEBp Ml\s[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDOSk3PwkJiYX7kJHAu\3EEoB?= NInUXWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyNFczPSd-MkSyNlA4OjV:L3G+
FaDu NUDncJJbTnWwY4Tpc44hSXO|YYm= MlrONkBp M4jmUYlvcGmkaYTzJJA3PSCneIDy[ZN{cW:wIHHu[EBjdG:la4OgWG5H|rFvaX7keYNm\CCWV1nTWEBmgHC{ZYPzbY9v MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{ME[yNkc,OjR{MkC2NlI9N2F-
IVD  M4DxUmZ2dmO2aX;uJGF{e2G7 NHfGVGkyOMLizszN NWPMcpRuOyCm MoHPdoV3\XK|ZYOgWG5HNc7z4pETcYVlcWG2ZXSgd5VxeHKnc4Ppc44hd2ZidHjlJIRqe2NibXH0dol5KG2jY4LvcY9t\WO3bHXzJIFo\3KnY3HuJIFv\CClb3zsZYdmdiCLSR?= M2W5eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUe2PFA5Lz5{NEG3OlgxQDxxYU6=
IVD  Mk\0SpVv[3Srb36gRZN{[Xl? NEO5UoQyOMLizszN NFHH[4w{KGR? Mnv1ZYJzd2ejdHXzJHRPTi4QsfMAl4lv\HWlZXSgeZAuemWpdXzheIlwdiCxZjDBSGFOXFNvNDDhcoQhSUSDTWTTMVXDqA>? NE\SRlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3OlgxQCd-MkSxO|Y5ODh:L3G+
iNKT MYPGeY5kfGmxbjDBd5NigQ>? M1\5UVExNzFyMDFOwG0> MYWwMlUhcA>? MVLpcohq[mm2czD0bIUhcW6mdXP0bY9vKG:oIFGyRXIhdVKQQTDhcoQhd3SqZYKg[oFkfG:{ NGDmRYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGyOFQ2Oyd-MkSxNlQ1PTN:L3G+
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THP-1  MVzGeY5kfGmxbjDBd5NigQ>? NViwXHJ[OTEEoN88US=> M2PNU|HDqGh? NV7hdlA3[WKxbHnzbIV{KHSqZTDl[oZm[3Rib3[gdmhUWDJ5IH;uJHNTNUFibWLORS=> NF;3S|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzPVM6QCd-MkO5N|k{QTh:L3G+
A549 MlzOSpVv[3Srb36gRZN{[Xl? M3;vOVHDqM7:TR?= M2jsb|Q5KGh? NVy1NIc4\W6qYX7j[ZMhfGinIIXwMZJm\3WuYYTpc44hd2ZiSd86RkBidmRic4Xid4VyfWWwdDDk[YNz\WG|ZTDpckBD[XhiZYjwdoV{e2mxbjDpcoR2[2WmIHL5JINwdWKrbnXkJJN1cW23bHH0bY9v MlnQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MECwPFAoRjJ|OUCwNFgxRC:jPh?=
A549 NFLXS3RCeG:ydH;zbZMhSXO|YYm= M1i2SVHDqM7:TR?= NVTIfXdvPDhiaB?= MmXhdoVlfWOnczD0bIUh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IHPvcYJqdmWmIIP0bY12dGG2aX;u MlTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MECwPFAoRjJ|OUCwNFgxRC:jPh?=
NCI-N87 M1juS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DtdVExNzJyL{OwJO69VQ>? NYD2XoNSPi9{NDDo M2rVS5N2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgd4lodmmoaXPhcpRtgQ>? NYjpRZJ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFY2PDVpPkKzPFQ3PTR3PD;hQi=>
AGS M{fLTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3j5[FExNzJyL{OwJO69VQ>? NFGyOHk3NzJ2IHi= NWKwXIR5e3WycILld5NmeyClZXzsJJZq[WKrbHn0fUB{cWewaX\pZ4FvfGy7 MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2NkW0OUc,OjN6NE[1OFU9N2F-
MGC80-3 NYPUNJpET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlL3NVAwOjBxM{Cg{txO M4DZbVYwOjRiaB?= MUHzeZBxemW|c3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> NIO1[Vk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0OlU1PSd-MkO4OFY2PDV:L3G+
HGC-27  M3HI[2Z2dmO2aX;uJGF{e2G7 M1TmN|cvPS9zNT:zNEDPxE1? NXS4[YgzPiCq MmfFbY5lfWOnczD0bIUh\GWyaH;zdIhwenmuYYTpc44h[W6mIIXwMZJm\3WuYYTpc44hd2ZiSd86Ru6y MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2NkW0OUc,OjN6NE[1OFU9N2F-
MGC80-3 NX\5RYIxTnWwY4Tpc44hSXO|YYm= MX63MlUwOTVxM{Cg{txO MnjGOkBp MVjpcoR2[2W|IITo[UBl\XCqb4PwbI9zgWyjdHnvckBidmRidYCtdoVofWyjdHnvckBw\iCLzsrC{tE> M33UcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OES2OVQ2Lz5{M{i0OlU1PTxxYU6=
HGC-27  NX7sWZFoSXCxcITvd4l{KEG|c3H5 MoPFO{42NzF3L{OwJO69VQ>? MYK2JIg> MlP4bY5lfWOnczDhdI9xfG:|aYOgbY4h[SC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NGKwPHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0OlU1PSd-MkO4OFY2PDV:L3G+
HBE  MV\GeY5kfGmxbjDBd5NigQ>? NHjvVI4yOM7:TR?= M1O4c|Np NUTXbZZw[WKxbHnzbIV{KHSqZTDpcoNz\WG|ZYOgc4YhUUxvNjDlfJBz\XO|aX;uJIlv\HWlZXSgZpkhS1OH NXjQ[GdsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NlQxQDlpPkKzPFI1ODh7PD;hQi=>
HepG2 M4rpOWZ2dmO2aX;uJGF{e2G7 MkLLNE4{NzFxMzFOwG0> M3;IcVQ5KGh? M{DqZZJm\HWlZYOgTWw3NWmwZIXj[YQhWE:QMTDlfJBz\XO|aX;u MnjFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUG4N|MoRjJ|N{mxPFM{RC:jPh?=
THP-1 NYTqdFRTTnWwY4Tpc44hSXO|YYm= M1e1fFUhyrWP M33nS|EhcA>? NWDrdFdITE2VTx?= Ml;4bY5pcWKrdIOgUXRDNWmwZIXj[YQhVkcQulKgZYN1cX[jdHnvci=> MlTEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4M{SyNVgoRjJ|NkO0NlE5RC:jPh?=
THP-1  MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILGeIs2KML3TR?= MWi0M|gh\A>? MWTEUXNQ MoLJdoVlfWOnczD0bIUhfmmjYnnsbZR6KG:oIHnueJJi[2WubIXsZZLDqE2WQh?= NX72b4Y2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2N|QzOThpPkKzOlM1OjF6PD;hQi=>
MDM MnjIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLWOUDDvU1? M{\tc|QwQCCm NHv6dHVFVVOR M1[wVpJm\HWlZYOgeIhmKH[rYXLpcIl1gSCxZjDpcpRz[WOnbHz1cIFzyqCPVFK= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ|NEKxPEc,OjN4M{SyNVg9N2F-
AM NGruWoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW[1JOK2VQ>? Mlm1OE85KGR? Ml3ESG1UVw>? MoPNdoVlfWOnczD0bIUhfmmjYnnsbZR6KG:oIHnueJJi[2WubIXsZZLDqE2WQh?= NYK2NWRTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2N|QzOThpPkKzOlM1OjF6PD;hQi=>
RAW 264  NHTDUGJHfW6ldHnvckBCe3OjeR?= NWLEOGR2OC5{LUWgxtVO MlqxN|AwPjBxOUCgcYlv NYf4SotKcW6qaXLpeJMhfGinIIDoc5NxcGG2YYPlJIFkfGm4aYT5JI9nKFCWUEHCxsA> MlvTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{izNFIoRjJ|NUe4N|AzRC:jPh?=
HUVEC M1XuS2Z2dmO2aX;uJGF{e2G7 NHz1VI4yOMLizszN MmDhNE42KGh? NIG1PY1FVVOR MmPUZ492dnSncnHjeIV{KHSqZTDsc5N{KG:oIGTp[VIhdVKQQR?= NHj4NGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W2N|Y{Oid-MkO1OlM3OzJ:L3G+
HT29 MWjGeY5kfGmxbjDBd5NigQ>? M2mw[|ExNzNyL{GwNEDPxE1? NV\HepJJOSCq MofPbY5pcWKrdHXzJIJwfGhiVGfFRWsucW6mdXPl[EBxOTByIIDyc4Nme3Orbne= M1P0N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK3NVU1Lz5{M{WyO|E2PDxxYU6=
HT29 NV3sUmE5TnWwY4Tpc44hSXO|YYm= NWfQRlhJOTBxM{CvNVAxKM7:TR?= NYrlUllUOSCq NXXKdXRbcW6qaXLpeJMhXE6ILXnu[JVk\WRicHjvd5Bpd3K7bHH0bY9vKGGwZDDk[Ydz[WSjdHnvckBw\iCLzsrC{tE> MnfkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MkexOVQoRjJ|NUK3NVU1RC:jPh?=
MM.1S MkmwRZBweHSxc3nzJGF{e2G7 NYe1fVN7OzBiwsXN NYDT[mhlOyCq NXnzXnpGcW6mdXPld{BOVSClZXzsJIRm[XSqIHnueo9tfmW|IH7lZ5Jwe2m| M2SzNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK3NVU1Lz5{M{WyO|E2PDxxYU6=
KMS-12-BM NFPzeJBCeG:ydH;zbZMhSXO|YYm= NYTNPZVUOzBiwsXN M1;WW|MhcA>? MlzibY5lfWOnczDNUUBk\WyuIHTlZZRpKGmwdn;seoV{KG6nY4Lvd4l{ Mo\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3MkexOVQoRjJ|NUK3NVU1RC:jPh?=
BAFs MUHGeY5kfGmxbjDBd5NigQ>? MnTsNE42NzIEoN88US=> NEDyRZczPCCq NWHSSZFocW6qaXLpeJMhXE6IzsGvSGVZKGmwZIXj[YQhS1mSMUnBNeKhfHKjboPjdolxfHN? NXXwWHUzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PFU1PTdpPkKzOFg2PDV5PD;hQi=>
SP6.5 NY\1N5RZTnWwY4Tpc44hSXO|YYm= MXe1JO69VQ>? NV7Zc2I1OiCq MlL3[IVkemWjc3XzxsB1emGwc3zvZ4F1cW:wIH;mJJA3PSCrbjD0bIUhdnWlbHX1d:Kh NWq2O4FJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFMxQDZpPkKzOFQ{ODh4PD;hQi=>
VUP NYKxeHRNTnWwY4Tpc44hSXO|YYm= Mk\sOUDPxE1? Mlj0NkBp MWDk[YNz\WG|ZYRCpJRz[W6|bH;jZZRqd25ib3[gdFY2KGmwIITo[UBvfWOuZYXzxsA> M3HrNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
OCM1 MYPGeY5kfGmxbjDBd5NigQ>? MUS1JO69VQ>? NHX4XHgzKGh? M{PJXIRm[3KnYYPld:KhfHKjboPsc4NifGmxbjDv[kBxPjViaX6geIhmKG63Y3zleZPDqA>? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OM431 NHrlNmFHfW6ldHnvckBCe3OjeR?= MWS1JO69VQ>? MXeyJIg> MmTy[IVkemWjc3XzxsB1emGwc3zvZ4F1cW:wIH;mJJA3PSCrbjD0bIUhdnWlbHX1d:Kh MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
SP6.5 MnjuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{[wdVIvPS1{MDFOwG0> M2n3Z|I1KGkEoB?= NIDZZodKSzVyPUWg{txONCCneHjpZol1eyC|dILvcoch[W62aT3wdo9tcW[ncnH0bZZmKGWoZnXjeJMhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> MmLxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEOwPFYoRjJ|NESzNFg3RC:jPh?=
VUP NV\qd2xQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTJNk42NTJyIN88US=> NYC1TlZnOjRiaNMg MUXJR|UxRTVizszNMEBmgGirYnn0d{B{fHKxbnegZY51cS2ycn;sbYZmemG2aY\lJIVn\mWldIOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OCM1 M4raRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDRS|ltOi53LUKwJO69VQ>? NFLxc3gzPCCqwrC= MXzJR|UxRTVizszNMEBmgGirYnn0d{B{fHKxbnegZY51cS2ycn;sbYZmemG2aY\lJIVn\mWldIOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MkHFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEOwPFYoRjJ|NESzNFg3RC:jPh?=
OM431 NHfHW4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M375[|IvPS1{MDFOwG0> M{LtVlI1KGkEoB?= M3ywdmlEPTB;NTFOwG0tKGW6aHnibZR{KHO2cn;u[{BidnSrLYDyc4xq\mW{YYTpeoUh\W[oZXP0d{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> M1nNSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
SP6.5 NEHLTodCeG:ydH;zbZMhSXO|YYm= NWDBW5lTPSEQvF2= MYmyOEBpyqB? MlvEbY5lfWOnczDhdI9xfG:|aYRCpC=> NYL4V|RnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFMxQDZpPkKzOFQ{ODh4PD;hQi=>
VUP MXvBdI9xfG:|aYOgRZN{[Xl? MkHPOUDPxE1? MlL1NlQhcMLi NXnEPVFicW6mdXPld{BieG:ydH;zbZPDqA>? NXr6UopPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFMxQDZpPkKzOFQ{ODh4PD;hQi=>
OCM1 MVnBdI9xfG:|aYOgRZN{[Xl? Moi5OUDPxE1? Mn;mNlQhcMLi M4Lr[Ylv\HWlZYOgZZBweHSxc3nzxsA> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OM431 NVjme5doSXCxcITvd4l{KEG|c3H5 M3rEdFUh|ryP M4LKd|I1KGkEoB?= M2rhU4lv\HWlZYOgZZBweHSxc3nzxsA> Mn3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEOwPFYoRjJ|NESzNFg3RC:jPh?=
SP6.5 NHK4WYpHfW6ldHnvckBCe3OjeR?= NHnYfXQ2KM7:TR?= MknxNVIhcA>? MXvy[YR2[2W|IITo[UBucWe{YYTpc47DqA>? MkTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEOwPFYoRjJ|NESzNFg3RC:jPh?=
VUP MUPGeY5kfGmxbjDBd5NigQ>? NF3EPFY2KM7:TR?= NXv0OVdWOTJiaB?= MmfHdoVlfWOnczD0bIUhdWmpcnH0bY9vyqB? NH6zPXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0N|A5Pid-MkO0OFMxQDZ:L3G+
OCM1 MV;GeY5kfGmxbjDBd5NigQ>? MnXtOUDPxE1? Mn;aNVIhcA>? MlT1doVlfWOnczD0bIUhdWmpcnH0bY9vyqB? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OM431 M4e1bGZ2dmO2aX;uJGF{e2G7 NHfs[G82KM7:TR?= NILNXGMyOiCq MVjy[YR2[2W|IITo[UBucWe{YYTpc47DqA>? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
HBL-1  MnjYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDFN:Kh|ryP MUCyOE81QC95MjDo NGTyXY9FVVOR M4m0U5Ntd3e|IHPlcIwh\3Kxd4ToJI1w\GW|dHz5 NE\odos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0NVc{OCd-MkO0OFE4OzB:L3G+
RAW 264.7 MlLJSpVv[3Srb36gRZN{[Xl? NVTpbYxGOi1zNTFOwG0> NFr3b|QyKGh? NUDUfVM6TE2VTx?= NYDMW25Xe3WycILld5NmeyC2aHWgZYN1cX[jdHnvckBw\iCLS1ug[oFucWy7IH3lcYJmenN? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2MUezNEc,OjN2NEG3N|A9N2F-
IL-1R  MUTGeY5kfGmxbjDBd5NigQ>? MVeyMVE2KM7:TR?= NELuVWkyKGh? MmfhSG1UVw>? Mkjld5VxeHKnc4Pld{B1cGViYXP0bZZifGmxbjDv[kBKU0tiZnHtbYx6KG2nbXLldpM> MoXvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEG3N|AoRjJ|NESxO|MxRC:jPh?=
RAW 264.7 MYXGeY5kfGmxbjDBd5NigQ>? NYCwVXYxOTVizszN NXu0R|M4OSCq NVrPdIh{TE2VTx?= NU\0b3Bte3WycILld5NmeyC2aHWgZYN1cX[jdHnvckBw\iCjbnSgTm5M M3zM[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESxO|MxLz5{M{S0NVc{ODxxYU6=
IL-1R  NYXSb5FbTnWwY4Tpc44hSXO|YYm= NVPCe|g3OTVizszN NUTCRWFLOSCq M3;2TGROW09? MYfzeZBxemW|c3XzJJRp\SCjY4TpeoF1cW:wIH;mJIFv\CCMTlu= Mn7YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEG3N|AoRjJ|NESxO|MxRC:jPh?=
U2OS MorKSpVv[3Srb36gRZN{[Xl? M4HqXFE2KM7:TR?= M{XnU|EhcA>? MnzsSG1UVw>? M3fkVpBz\X[nboTzJJRp\SCOUGOtJI9zKEmOLUGtd5RqdXWuYYTl[EBnd3KvYYTpc44hd2ZiS{[zMZBW[iClaHHpcpPDqA>? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2MUezNEc,OjN2NEG3N|A9N2F-
MT‐1  MXHGeY5kfGmxbjDBd5NigQ>? MUS4xsDDvW1? M2r3VVMhcA>? M4rSOIRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIIFihLBUXEGWMzDhcoQheOLCkETFRnAy MnPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{N{i0O|koRjJ|Mke4OFc6RC:jPh?=
MT‐2  MorqSpVv[3Srb36gRZN{[Xl? M2TUW|jDqML3bR?= NVvnWIVnOyCq NE\Oe|Jl\WO{ZXHz[ZMhfGinIHzleoVteyCxZjDw5qCRW1SDVEOgZY5lKHEkgKC0SWJROQ>? NXnEbXdWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO|g1PzlpPkKzNlc5PDd7PD;hQi=>
MT‐1  NEH0OZRHfW6ldHnvckBCe3OjeR?= M1vTNFjDqML3bR?= M1y0ZlMhcA>? NET2PHFl\WO{ZXHz[ZMhfGinIHzleoVteyCxZjD0bIUheDZ3IIP1ZpVvcXRib3[gUmbjiJEQulNCpC=> NU\5UYhkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO|g1PzlpPkKzNlc5PDd7PD;hQi=>
MT‐2  MX;GeY5kfGmxbjDBd5NigQ>? MoHvPOKhyrWv NXfxSXBrOyCq NVT2NmxF\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[geIhmKHB4NTDzeYJ2dmm2IH;mJG5H6oDSzsrCxsA> NEixOlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K3PFQ4QSd-MkOyO|g1Pzl:L3G+
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Assay
Methods Test Index PMID
Western blot NF-κB p-IKKβ/ IκBα p-IRAK4 / IRAK4 NF-κB p-IKKβ/ IκBα p-IRAK4 / IRAK4 31332209 23441730
Growth inhibition assay Cell viability Cell viability 31332209

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[2]

  • Cell lines: NCI-H1703 细胞
  • Concentrations: ~8 μM
  • Incubation Time: 12 小时
  • Method:

    细胞在96孔板中转染siRNA,然后在完全NSCLC 培养基中培养72小时,再使用BAY 11-7082 处理12小时。细胞与[3H]胸甘温育3小时。使用自动细胞收集器将细胞收集到过滤器上,然后通过β-闪烁计数法测量放射性。

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+corn oil

8mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 207.25
化学式

C10H9NO2S

CAS号 19542-67-7
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

* 必填项

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