BAY 11-7082

For research use only. Not for use in humans.

目录号:S2913 别名: BAY 11-7821

BAY 11-7082 Chemical Structure

CAS No. 19542-67-7

BAY 11-7082 (BAY 11-7821)是一种 NF-κB 抑制剂,抑制TNFα诱导的 IκBα 磷酸化,在肿瘤细胞中IC50为10 μM。BAY 11-7082 可抑制 ubiquitin-specific protease USP7USP21,对应的IC50值分别为0.19 μM和0.96 μM。BAY 11-7082 在胃癌细胞中可诱导凋亡和S期阻滞。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 988.44 现货
RMB 738.58 现货
RMB 2223.41 现货
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客户使用Selleck生产的BAY 11-7082发表文献253篇:

产品安全说明书

IκB/IKK抑制剂选择性比较

生物活性

产品描述 BAY 11-7082 (BAY 11-7821)是一种 NF-κB 抑制剂,抑制TNFα诱导的 IκBα 磷酸化,在肿瘤细胞中IC50为10 μM。BAY 11-7082 可抑制 ubiquitin-specific protease USP7USP21,对应的IC50值分别为0.19 μM和0.96 μM。BAY 11-7082 在胃癌细胞中可诱导凋亡和S期阻滞。
靶点
E2-conjugating enzymes [6]
(Cell-free assay)
USP7 [7]
(Cell-free assay)
USP21 [7]
(Cell-free assay)
USP6 [7]
(Cell-free assay)
IκBα phosphorylation [1]
(Tumor cells)
0.19 μM 0.96 μM 1.7 μM 10 μM
体外研究

BAY 11-7082完全且特异性地废除 NF-κB DNA结合, 下调NF-κB-诱导的细胞因子IL-6,且诱导细胞凋亡。[1] BAY 11-7082 (< 8 μM) 有效抑制原有的和TNFα 刺激的NF-κB荧光素酶活性,这种作用存在剂量依赖性。BAY 11-7082 (8 μM) 强抑制NCI-H1703 细胞增殖。 [2] Bay 11-7082 (5 μM)作用于感染HTLV-I的T细胞系,快速且有效的降低NF-κB的DNA结合力,且下调抗凋亡基因Bcl-x(L)的表达,而对另一种转录因子AP-1与DNA结合没有作用效果。Bay 11-7082诱导原代ATL细胞发生凋亡比正常的外周血单核细胞更明显,而诱导的这些细胞凋亡也与NF-κB活性的下调相关。Bay 11-7082 (5 μM) 选择性诱导感染HTLV-I的T细胞系发生细胞凋亡,且下调 cyclin D1, cyclin D2, 和 Bcl-xL的表达。 [3] BAY 11-7082 (100 μM)作用于小鼠海马脑片区,抑制NMDA引起的p65发生 核易位,也抑制NMDA诱导的NF-κB结合力的增强。BAY 11-7082抑制NMDA毒性发生在海马脑片区的CA1区,20 μM时,对40%神经有保护作用,100 μM时,对70%神经有保护作用。[4]作用于脂肪组织,实验中所有浓度的BAY 11-7082都显著抑制 NF-κB p65 DNA结合活性,而作用于骨骼肌,只有50 μM 和 100 μM 的BAY 11-7082显著抑制NF-κB p65 DNA结合活性。BAY 11-7082 (100 μM) 作用于人体脂肪组织和骨骼肌,降低IKK-β蛋白。BAY 11-7082 (100 μM) 显著降低TNF-α从动物脂肪组织中释放,而 IL-6 和 IL-8 的释放显著抑制实验中BAY 11-7082的所有浓度。 BAY 11-7082 (50 μM)作用于骨骼肌,显著降低 TNF-α, IL-6, 和IL-8 的释放。[5] 此外,BAY 11-7082还被发现抑制了 E2-conjugating酶和E3 ligase酶的活性,从而诱导了B-淋巴癌细胞和T淋巴癌细胞的死亡。 [6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa NGm3SlRHfW6ldHnvckBCe3OjeR?= NWi2PGxCOTEEoN88US=> MlPKNU42KGh? M2C0XYFjd2yrc3jld{BDWEFiaX7keYNm\CC3cDDy[Yd2dGG2aX;uJI9nKE[QIHHu[EBOVVBvOdMg MoWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5OUe0N|coRjJ3N{m3OFM4RC:jPh?=
SiHa  MWjGeY5kfGmxbjDBd5NigQ>? MXexNOKh|ryP NFjXSIEyNjViaB?= Mnz1ZYJwdGm|aHXzJGJRSSCrbnT1Z4VlKHWyIILl[5Vt[XSrb36gc4YhTk5iYX7kJG1OWC17wrC= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd7N{SzO{c,OjV5OUe0N|c9N2F-
ARPE-19 NW\GTnpPTnWwY4Tpc44hSXO|YYm= Moj6NUDPxE1? NVz0VnNyOC53IHi= MnTMd5VxeHKnc4Pld{BVTy2rbnT1Z4VlyqCLTD24xsBxem:vb4TldkBi[3SrdnH0bY9v M3\qelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUmzNFI6Lz5{NUW5N|AzQTxxYU6=
HCT116 NV\0bZFQTnWwY4Tpc44hSXO|YYm= M17ob|Uh|ryP M2HSTVIhcA>? MlW4SG1UVw>? MYrheJRmdnWjdHXzJJNqdHmvYYLpck1qdmS3Y3XkJIRwf26{ZXf1cIF1cW:wIH;mJIN6[2yrbjDENS=> NHjGVII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS3PVczOyd-MkW0O|k4OjN:L3G+
HMECs M4f1WWZ2dmO2aX;uJGF{e2G7 NFLabHg2yqEQvF2= MmjhNkBp NEL4No1i[m:uaYPo[ZMhXE6ILd8xMYlv\HWlZXSgWmNCVS1zIHX4dJJme3Orb39CpC=> MlyxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzOUOxNVYoRjJ3MUmzNVE3RC:jPh?=
A549  M3z2WGZ2dmO2aX;uJGF{e2G7 M3\VS|Ex6oDLwsXN NYPlTFc5OTJiaB?= NV\U[Flle3WycILld5NmeyCGdnytN{BqdmS3Y3XkJIFkfGm4YYTpc44hd2ZicE[1 MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF3NkiwNEc,OjVzNU[4NFA9N2F-
RAW 264.7 MkHESpVv[3Srb36gRZN{[Xl? NFnyemI2yqEQvF2= NH7IbYUyKGh? NXLWU2c{cW6qaXLpeJMhXE6ILd8xJIFv\CCLTD2xNkBxPDBicILv[JVkfGmxbh?= NFzwNG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxPVU3Pyd-MkWwNVk2Pjd:L3G+
macrophages MnPkSpVv[3Srb36gRZN{[Xl? MnH5OUDDvU1? M2XLXFMhcA>? MXjwZZJ1cWGubImgZoxw[2u|IGnQSnMucW6mdXPl[EBmgHC{ZYPzbY9vKG:oIHnOU3Mh[W6mIFPPXE0zyqB? MmrhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7Nke4PVgoRjJ2OU[3PFk5RC:jPh?=
HUVECs NGLrNppHfW6ldHnvckBCe3OjeR?= NIHCe|M{NTNyIN88US=> Mn\NNUBp NV[4N5NKemWmdXPld{B1cGViZYjwdoV{e2mxbjDv[kBucVJvMUS2ZUBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh4M{m2OUc,OjR6NkO5OlU9N2F-
HeLa  MVjGeY5kfGmxbjDBd5NigQ>? NWHCdYJ1PcLizszN NEnVfW4zPCCq NWSyUJM4TE2VTx?= NIrNXY5z\WS3Y3XzJJRp\SCjY4Tpeol1gSCxZjDUUmYu|rFicILvcY91\XMEoB?= NIDXd5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[1O|c5Oyd-MkS2OVc4QDN:L3G+
A549 NXXZTmJwTnWwY4Tpc44hSXO|YYm= MX2xNEDPxE1? NX7jfXhkOeLCiXi= NUPIRWVIcW6qaXLpeJMhfGinIHnuZ5Jm[XOnIH;mJJBpd3OyaH:tTe67Ss7zIHnuJHBCOTB|LXnu[oVkfGWmIHP1cJR2emW|wrC= M3vyTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkGyOFg5Lz5{NE[xNlQ5QDxxYU6=
HUVEC M3vjOmZ2dmO2aX;uJGF{e2G7 M32zV|IxKML3TR?= M4\JcFAvPSCq MWfEUXNQ NYLYUGhIeHKndnXueJMhfGinIHnu[JVkfGmxbjDv[kBGSU1iZYjwdoV{e2mxbh?= NWXSSZYxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1OVEzODlpPkK0OVUyOjB7PD;hQi=>
A549RT-eto MVPBdI9xfG:|aYOgRZN{[Xl? MX:xNOKh|ryP MV[yOEBp NGDrPVRFVVOR MV7hZ4NmdGW{YYTld{BHTVKRLX3l[IlifGWmIHHwc5B1d3Orcx?= NGOxNGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWzOVA5Oyd-MkS1N|UxQDN:L3G+
THP-1  NF7QU4RHfW6ldHnvckBCe3OjeR?= NIPldFQxNjFxMdMg{txO NELI[3cxNjViaB?= M{T3S4Fjem:pYYTld{BVVkZvzsGgd4VkemW2aX;uJIF{KHenbHygZZMhfGinIHnuZ5Jm[XOnZDDz[YNz\XSrb36gc4YhUUxvNjDhcoQhUUxvMd8y Ml7VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|N{i1N|YoRjJ2M{e4OVM3RC:jPh?=
SKCXCR2  MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4D5T|IhyrWP MUi0PEBp Mnrk[IVkemWjc3XzJINmdGxicILvcIln\XKjdHnvckB{cWewaX\pZ4FvfGy7 Ml7UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|N{[3OFcoRjJ2M{e2O|Q4RC:jPh?=
SKCXCR2  NGnXZXVHfW6ldHnvckBCe3OjeR?= NGi2eIwzKML3TR?= Mk\iOFghcA>? NHfOZVhjdG:la4OgeIhmKEO[Q1yxMYlv\HWlZXSgZ4VtdCCrbo\hd4lwdg>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN5Nke0O{c,OjR|N{[3OFc9N2F-
OVCXCR2 MUHGeY5kfGmxbjDBd5NigQ>? Mo\VNkDDvU1? NXPpVmxbPDhiaB?= M320V4Jtd2OtczD0bIUhS1iFTEGtbY5lfWOnZDDj[YxtKGmwdnHzbY9v MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN5Nke0O{c,OjR|N{[3OFc9N2F-
DSCs  NHzPcFdHfW6ldHnvckBCe3OjeR?= Mn\KNk42KM7:TR?= MlK2NE42KGh? M3P1cZJmfmW{c3XzJJRp\SCnbnjhcoNmdWWwdDDv[kBES0x{L1PDVlIh\XiycnXzd4lwdiCxZjDEV2N{KGmwZIXj[YQh[nliSVytN|M> NXvzZXlrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOFQzPDBpPkK0N|Q1OjRyPD;hQi=>
WPs NHP6Z2hHfW6ldHnvckBCe3OjeR?= MV2yOUDPxE1? NGXSTXo2KG2rbh?= NH[we4l{fXCycnXzd4V{KEGWUDDhcoQhfleIIIPlZ5JmfGmxbh?= NHzuR289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOzNVIxPyd-MkSzN|EzODd:L3G+
A549RT-eto MkTsRZBweHSxc3nzJGF{e2G7 M376RlExyqEQvF2= Moj3NlQhcA>? NIjGe5Ri[2OnbHXyZZRmeyCIMUSg[Zh1emGldD3t[YRq[XSnZDDhdI9xfG:|aYOgZ49u[mmwZXSgeJJm[XSvZX70JJdqfGhiRkG0 MnrSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkC3NlUoRjJ2MkKwO|I2RC:jPh?=
A549RT-eto MmrtSpVv[3Srb36gRZN{[Xl? Mlr5NVDDqM7:TR?= NE\SOokzPCCq MoSy[IVkemWjc3XzJJRp\SCneIDy[ZN{cW:wIHzleoVteyCxZjDOSk3PwkJiYX7kJHAu\3EEoB?= NITJd4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyNFczPSd-MkSyNlA4OjV:L3G+
FaDu MVPGeY5kfGmxbjDBd5NigQ>? MXOyJIg> MWrpcohq[mm2czDwOlUh\XiycnXzd4lwdiCjbnSgZoxw[2u|IGTOSu6yNWmwZIXj[YQhXFeLU2Sg[ZhxemW|c3nvci=> MmTXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkC2NlIoRjJ2MkKwOlIzRC:jPh?=
IVD  MWnGeY5kfGmxbjDBd5NigQ>? NIm0[ZkyOMLizszN MVSzJIQ> NYDTdYhNemW4ZYLz[ZMhXE6ILd8x5qCUdWWmaXH0[YQhe3WycILld5Nqd25ib3[geIhmKGSrc3OgcYF1emm6IH3hZ5JwdW:uZXP1cIV{KGGpZ4LlZ4FvKGGwZDDjc4xt[WenbjDJTS=> Mk[5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{[4NFgoRjJ2MUe2PFA5RC:jPh?=
IVD  MnTJSpVv[3Srb36gRZN{[Xl? NYWxZXBSOTEEoN88US=> MWizJIQ> M3jtPIFjem:pYYTld{BVVkZvzsJihLNqdmS3Y3XkJJVxNXKnZ4XsZZRqd25ib3[gRWRCVVSVLUSgZY5lKEGGQV3UV{02yqB? NELle|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3OlgxQCd-MkSxO|Y5ODh:L3G+
iNKT MmTBSpVv[3Srb36gRZN{[Xl? MmnrNVAwOTByIN88US=> M3LmNlAvPSCq NH7K[29qdmirYnn0d{B1cGViaX7keYN1cW:wIH;mJGEzSVJibWLORUBidmRib4To[ZIh\mGldH;y NXrpVJFGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNlQ1PTNpPkK0NVI1PDV|PD;hQi=>
PC-3 NFThTpdHfW6ldHnvckBCe3OjeR?= M1ToS|IvPS93L{GwJO69VQ>? MXmwMlUhcA>? NFvGVXJjdG:la4OgTWdHNUmLLXnu[JVk\WRiU2TTJI1TVkFiZYjwdoV{e2mxbtMg MnXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNUW1NlAoRjJ2MEW1OVIxRC:jPh?=
THP-1  NVLnOWNbTnWwY4Tpc44hSXO|YYm= MlvYNVDDqM7:TR?= M4nBclHDqGh? M{f0fYFjd2yrc3jld{B1cGViZX\m[YN1KG:oIILIV3AzPyCxbjDTVk1CKG2UTlG= NXPsV5NSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5N|k{QThpPkKzPVM6Ozl6PD;hQi=>
A549 MWnGeY5kfGmxbjDBd5NigQ>? NY\PdmtpOcLizszN MkL5OFghcA>? MoW5[Y5p[W6lZYOgeIhmKHWyLYLl[5Vt[XSrb36gc4YhUc78QjDhcoQhe3Wkc3XxeYVvfCCmZXPy[YF{\SCrbjDCZZgh\XiycnXzd4lwdiCrbnT1Z4VlKGK7IHPvcYJqdmWmIIP0bY12dGG2aX;u NYfF[YpmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFAxQDBpPkKzPVAxODhyPD;hQi=>
A549 MXPBdI9xfG:|aYOgRZN{[Xl? M4fBeVHDqM7:TR?= MmrmOFghcA>? MknodoVlfWOnczD0bIUh[2WubDDk[YF1cCCrbnT1Z4VlKGK7IHPvcYJqdmWmIIP0bY12dGG2aX;u M3\LRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUCwNFgxLz5{M{mwNFA5ODxxYU6=
NCI-N87 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVOxNE8zOC9|MDFOwG0> Mlv2Ok8zPCCq MUHzeZBxemW|c3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> MlvSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NE[1OFUoRjJ|OES2OVQ2RC:jPh?=
AGS NEmweI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2r6fVExNzJyL{OwJO69VQ>? MXG2M|I1KGh? MX;zeZBxemW|c3XzJINmdGxidnnhZoltcXS7IIPp[45q\mmlYX70cJk> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2NkW0OUc,OjN6NE[1OFU9N2F-
MGC80-3 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\KTVExNzJyL{OwJO69VQ>? NHvwbos3NzJ2IHi= M{L0O5N2eHC{ZYPz[ZMh[2WubDD2bYFjcWyrdImgd4lodmmoaXPhcpRtgQ>? NX\RbmV2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFY2PDVpPkKzPFQ3PTR3PD;hQi=>
HGC-27  M4rrc2Z2dmO2aX;uJGF{e2G7 M{HKV|cvPS9zNT:zNEDPxE1? M13sPFYhcA>? NXHBPIEycW6mdXPld{B1cGViZHXwbI9{eGixconsZZRqd25iYX7kJJVxNXKnZ4XsZZRqd25ib3[gTe67Ss7z MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2NkW0OUc,OjN6NE[1OFU9N2F-
MGC80-3 MnXxSpVv[3Srb36gRZN{[Xl? MojRO{42NzF3L{OwJO69VQ>? M3vrTlYhcA>? MXvpcoR2[2W|IITo[UBl\XCqb4PwbI9zgWyjdHnvckBidmRidYCtdoVofWyjdHnvckBw\iCLzsrC{tE> NFzxNJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0OlU1PSd-MkO4OFY2PDV:L3G+
HGC-27  NH\rbWtCeG:ydH;zbZMhSXO|YYm= MWm3MlUwOTVxM{Cg{txO M1;yUFYhcA>? NUnnTGpJcW6mdXPld{BieG:ydH;zbZMhcW5iYTD0bY1mNSCjbnSg[I9{\S2mZYDlcoRmdnRibXHucoVz NU[xSpdDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OFY2PDVpPkKzPFQ3PTR3PD;hQi=>
HBE  MYDGeY5kfGmxbjDBd5NigQ>? M1fF[lEx|ryP MXOzbC=> NGHNVWNi[m:uaYPo[ZMhfGinIHnuZ5Jm[XOnczDv[kBKVC14IHX4dJJme3Orb36gbY5lfWOnZDDifUBEW0V? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NEC4PUc,OjN6MkSwPFk9N2F-
HepG2 NVzCfmlsTnWwY4Tpc44hSXO|YYm= NYXZNXp[OC5|L{GvN{DPxE1? NX\BSWQ3PDhiaB?= NXPaSnB[emWmdXPld{BKVDZvaX7keYNm\CCST16xJIV5eHKnc4Ppc44> M1r3TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{mxPFM{Lz5{M{e5NVg{OzxxYU6=
THP-1 NFHJe2pHfW6ldHnvckBCe3OjeR?= MYK1JOK2VQ>? MojZNUBp M37T[WROW09? NXfQU3YzcW6qaXLpeJMhVVSELXnu[JVk\WRiTldOvmIh[WO2aY\heIlwdg>? Mk\1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4M{SyNVgoRjJ|NkO0NlE5RC:jPh?=
THP-1  MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYSycZRqPSEEtV2= M3Hwe|QwQCCm M4XDfWROW09? NV\K[5IzemWmdXPld{B1cGVidnnhZoltcXS7IH;mJIlvfHKjY3XscJVt[XMEoF3URi=> NVX3fHU1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2N|QzOThpPkKzOlM1OjF6PD;hQi=>
MDM MoDsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\ldnhLPSEEtV2= Mki1OE85KGR? M{O2S2ROW09? NHq0eWpz\WS3Y3XzJJRp\SC4aXHibYxqfHlib3[gbY51emGlZXzseYxiesLiTWTC M1K0XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkO0NlE5Lz5{M{[zOFIyQDxxYU6=
AM NXXFOYNRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVu1JOK2VQ>? M2L3SVQwQCCm NXHETYF7TE2VTx?= MkPkdoVlfWOnczD0bIUhfmmjYnnsbZR6KG:oIHnueJJi[2WubIXsZZLDqE2WQh?= NHHsTlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[zOFIyQCd-MkO2N|QzOTh:L3G+
RAW 264  MkXaSpVv[3Srb36gRZN{[Xl? MojxNE4zNTViwsXN Mlv3N|AwPjBxOUCgcYlv MXPpcohq[mm2czD0bIUheGixc4DoZZRie2ViYXP0bZZqfHlib3[gVHRROUMEoB?= MmS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{izNFIoRjJ|NUe4N|AzRC:jPh?=
HUVEC MWPGeY5kfGmxbjDBd5NigQ>? M1r2[FExyqEQvF2= M{PPSVAvPSCq MkHvSG1UVw>? MW\jc5VvfGW{YXP0[ZMhfGinIHzvd5Mhd2ZiVHnlNkBuWk6D MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV4M{[zNkc,OjN3NkO2N|I9N2F-
HT29 NUfVWIo2TnWwY4Tpc44hSXO|YYm= MV:xNE8{OC9zMECg{txO NETUZogyKGh? MX3pcohq[mm2ZYOgZo91cCCWV1XBT{1qdmS3Y3XkJJAyODBicILvZ4V{e2mwZx?= NYjS[I1WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1NlcyPTRpPkKzOVI4OTV2PD;hQi=>
HT29 NHrWV3VHfW6ldHnvckBCe3OjeR?= NF\4VowyOC9|MD:xNFAh|ryP MVexJIg> MUDpcohq[mm2czDUUmYucW6mdXPl[EBxcG:|cHjvdplt[XSrb36gZY5lKGSnZ4Lh[IF1cW:wIH;mJGnPwkMQsR?= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV{N{G1OEc,OjN3MkexOVQ9N2F-
MM.1S NWPWNFN{SXCxcITvd4l{KEG|c3H5 M37Dd|MxKML3TR?= NYX5fJF2OyCq M3nrXIlv\HWlZYOgUW0h[2WubDDk[YF1cCCrbo\vcJZmeyCwZXPyc5Nqew>? M2LQVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK3NVU1Lz5{M{WyO|E2PDxxYU6=
KMS-12-BM NU\zTZNrSXCxcITvd4l{KEG|c3H5 MoX2N|AhyrWP M4PhWFMhcA>? NYTQTWxycW6mdXPld{BOVSClZXzsJIRm[XSqIHnueo9tfmW|IH7lZ5Jwe2m| M3XDb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUK3NVU1Lz5{M{WyO|E2PDxxYU6=
BAFs Ml3lSpVv[3Srb36gRZN{[Xl? NEHvdWUxNjVxMdMg{txO NVT5PHBJOjRiaB?= NHu0[3JqdmirYnn0d{BVVkcQsT;ESXghcW6mdXPl[EBEYVBzOVGxxsB1emGwc3PybZB1ew>? MlHGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OEW0OVcoRjJ|NEi1OFU4RC:jPh?=
SP6.5 NYLncYlkTnWwY4Tpc44hSXO|YYm= NE[0SIw2KM7:TR?= M4\2U|IhcA>? M{m5foRm[3KnYYPld:KhfHKjboPsc4NifGmxbjDv[kBxPjViaX6geIhmKG63Y3zleZPDqA>? NGLJZWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0N|A5Pid-MkO0OFMxQDZ:L3G+
VUP MmDtSpVv[3Srb36gRZN{[Xl? MlOzOUDPxE1? MlPzNkBp MmHa[IVkemWjc3XzxsB1emGwc3zvZ4F1cW:wIH;mJJA3PSCrbjD0bIUhdnWlbHX1d:Kh MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OCM1 MoLISpVv[3Srb36gRZN{[Xl? M1SxWVUh|ryP MYGyJIg> MVnk[YNz\WG|ZYRCpJRz[W6|bH;jZZRqd25ib3[gdFY2KGmwIITo[UBvfWOuZYXzxsA> NGmzSWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0N|A5Pid-MkO0OFMxQDZ:L3G+
OM431 NF\oZ5BHfW6ldHnvckBCe3OjeR?= Ml\ZOUDPxE1? MYOyJIg> NWDrNFdL\GWlcnXhd4V{yqC2cnHud4xw[2G2aX;uJI9nKHB4NTDpckB1cGViboXjcIV2e8Li M2ni[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
SP6.5 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVuyMlUuOjBizszN M4SxN|I1KGkEoB?= NVPMN4REUUN3ME21JO69VSxiZYjobYJqfHNic4Tyc45oKGGwdHmtdJJwdGmoZYLheIl3\SCnZn\lZ5R{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NF7tPGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0N|A5Pid-MkO0OFMxQDZ:L3G+
VUP NXX5S48{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvUXGxTOi53LUKwJO69VQ>? NYjZTlBjOjRiaNMg MlvuTWM2OD13IN88UUwh\XiqaXLpeJMhe3S{b37nJIFvfGlvcILvcIln\XKjdHn2[UBm\m[nY4TzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OCM1 M{\3cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TLeVIvPS1{MDFOwG0> M3\QflI1KGkEoB?= NW[2WlJWUUN3ME21JO69VSxiZYjobYJqfHNic4Tyc45oKGGwdHmtdJJwdGmoZYLheIl3\SCnZn\lZ5R{KGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NHvQXVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0N|A5Pid-MkO0OFMxQDZ:L3G+
OM431 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWWyMlUuOjBizszN NWO2cY54OjRiaNMg NHTCUohKSzVyPUWg{txONCCneHjpZol1eyC|dILvcoch[W62aT3wdo9tcW[ncnH0bZZmKGWoZnXjeJMhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NHHsRXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S0N|A5Pid-MkO0OFMxQDZ:L3G+
SP6.5 NFz1Om1CeG:ydH;zbZMhSXO|YYm= NV23O4VpPSEQvF2= M2ixNlI1KGkEoB?= M3Xrcolv\HWlZYOgZZBweHSxc3nzxsA> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
VUP MkXERZBweHSxc3nzJGF{e2G7 M4LDcFUh|ryP Ml32NlQhcMLi MoPnbY5lfWOnczDhdI9xfG:|aYRCpC=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OCM1 M13xXWFxd3C2b4Ppd{BCe3OjeR?= MmrFOUDPxE1? NIjt[pIzPCCqwrC= M3S3fYlv\HWlZYOgZZBweHSxc3nzxsA> M1TVOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
OM431 NVTBOJprSXCxcITvd4l{KEG|c3H5 NXjL[VNXPSEQvF2= MoDSNlQhcMLi NVXUemFJcW6mdXPld{BieG:ydH;zbZPDqA>? M1rSTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
SP6.5 NEXKU3NHfW6ldHnvckBCe3OjeR?= NFXmNow2KM7:TR?= M4HzOVEzKGh? MoqxdoVlfWOnczD0bIUhdWmpcnH0bY9vyqB? NVXETopoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFMxQDZpPkKzOFQ{ODh4PD;hQi=>
VUP M4jwXWZ2dmO2aX;uJGF{e2G7 MYe1JO69VQ>? NHrn[4kyOiCq MXXy[YR2[2W|IITo[UBucWe{YYTpc47DqA>? NWOwUYdMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFMxQDZpPkKzOFQ{ODh4PD;hQi=>
OCM1 MV7GeY5kfGmxbjDBd5NigQ>? NUCwT|VYPSEQvF2= NILXdFMyOiCq MnLEdoVlfWOnczD0bIUhdWmpcnH0bY9vyqB? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2M{C4Okc,OjN2NEOwPFY9N2F-
OM431 MlrWSpVv[3Srb36gRZN{[Xl? MXS1JO69VQ>? M2jXUlEzKGh? MWjy[YR2[2W|IITo[UBucWe{YYTpc47DqA>? M1vs[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NESzNFg3Lz5{M{S0N|A5PjxxYU6=
HBL-1  M4LBPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrIN:Kh|ryP MWWyOE81QC95MjDo NYXMeW5OTE2VTx?= M1frT5Ntd3e|IHPlcIwh\3Kxd4ToJI1w\GW|dHz5 MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR2MUezNEc,OjN2NEG3N|A9N2F-
RAW 264.7 NHLGPZdHfW6ldHnvckBCe3OjeR?= MkTHNk0yPSEQvF2= MXOxJIg> NVjxOYM3TE2VTx?= NYS2UJY2e3WycILld5NmeyC2aHWgZYN1cX[jdHnvckBw\iCLS1ug[oFucWy7IH3lcYJmenN? Ml7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEG3N|AoRjJ|NESxO|MxRC:jPh?=
IL-1R  MXXGeY5kfGmxbjDBd5NigQ>? M{XGWFIuOTVizszN Ml76NUBp NUTjZ41rTE2VTx?= M4rZRZN2eHC{ZYPz[ZMhfGinIHHjeIl3[XSrb36gc4YhUUuNIH\hcYltgSCvZX3i[ZJ{ MlriQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEG3N|AoRjJ|NESxO|MxRC:jPh?=
RAW 264.7 NXH6cHhITnWwY4Tpc44hSXO|YYm= MonsNVUh|ryP Mkf2NUBp NGn3[JFFVVOR NWfrSGROe3WycILld5NmeyC2aHWgZYN1cX[jdHnvckBw\iCjbnSgTm5M MnXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NEG3N|AoRjJ|NESxO|MxRC:jPh?=
IL-1R  NWDveJZwTnWwY4Tpc44hSXO|YYm= NYDPe3ZrOTVizszN MorvNUBp MkDQSG1UVw>? MkPJd5VxeHKnc4Pld{B1cGViYXP0bZZifGmxbjDv[kBidmRiSl7L NVHENmNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFE4OzBpPkKzOFQyPzNyPD;hQi=>
U2OS MXPGeY5kfGmxbjDBd5NigQ>? Ml;2NVUh|ryP MkHqNUBp MUfEUXNQ NXXBNnhSeHKndnXueJMhfGinIFzQV{0hd3JiSVytNU1{fGmvdXzheIVlKG[xcn3heIlwdiCxZjDLOlMueFWkIHPoZYlve8Li NYLtVIw6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OFE4OzBpPkKzOFQyPzNyPD;hQi=>
MT‐1  MWLGeY5kfGmxbjDBd5NigQ>? NX\XR4JNQMLiwsXt MY[zJIg> NXP1R2VX\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[gdQKBmFOWQWSzJIFv\CCy4pEQOGVDWDF? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ5OES3PUc,OjN{N{i0O|k9N2F-
MT‐2  MmX6SpVv[3Srb36gRZN{[Xl? NGn4XVM5yqEEtX2= NH;uSJc{KGh? Mmm4[IVkemWjc3XzJJRp\SCuZY\lcJMhd2ZicPMAlHNVSVR|IHHu[EBx6oDSNFXCVFE> NUftS2V1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyO|g1PzlpPkKzNlc5PDd7PD;hQi=>
MT‐1  NWLlfGV{TnWwY4Tpc44hSXO|YYm= M{m4RVjDqML3bR?= MnTvN{Bp NXHOVIpN\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[geIhmKHB4NTDzeYJ2dmm2IH;mJG5H6oDSzsrCxsA> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ5OES3PUc,OjN{N{i0O|k9N2F-
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP (see the Experimental section). The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

NF-κB; 

PubMed: 31332209     


(A,B) Effect of Bay 11-7082 at indicated concentrations for 12 h on inhibition of NF-κB protein expression was examined by Western blot. *P < 0.05 vs. con group.

p-IKKβ/ IκBα ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h with the indicated concentrations of BAY 11-7082 and then stimulated for 15 min with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were denatured in SDS, subjected to SDS/PAGE, and immunoblotted with antibodies that recognize the active phosphorylated form of IKKβ, the IKKβ substrate p105 phosphorylated at Ser933 and all forms of IκBα and GAPDH.

p-IRAK4 / IRAK4 ; 

PubMed: 23441730     


Murine RAW 264.7 macrophages were incubated for 1 h without (−) or with (+) 15 μM BAY 11-7082 and then stimulated for the times indicated with 100 ng/ml LPS. The cells were lysed and aliquots of the cell extract (20 μg of protein) were subjected to SDS/PAGE and immunoblotting (bottom three panels). IRAK4 was immunoprecipitated (IP) from 2 mg of cell extract protein using 2 μg of anti-IRAK4 antibody using the procedure used to immunoprecipitate HOIP. The immunoprecipitates were denatured in SDS, subjected to SDS/PAGE and immunoblotted with an antibody that recognizes IRAK4 phosphorylated at Thr346 and Ser346 (p-IRAK4) and an antibody that recognizes all forms of IRAK4. 

31332209 23441730
Growth inhibition assay
Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

Cell viability; 

PubMed: 31332209     


(C) Cell viability with Bay 11-7082 at indicated concentrations for 12 h was detected by MTT assay. *P < 0.05 vs. con group; #P < 0.05 vs. 1 µM Bay; &P < 0.05 vs. 3 µM Bay; $P < 0.05 vs. 5 µM Bay. 

31332209

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[2]

- 合并
  • Cell lines: NCI-H1703 细胞
  • Concentrations: ~8 μM
  • Incubation Time: 12 小时
  • Method:

    细胞在96孔板中转染siRNA,然后在完全NSCLC 培养基中培养72小时,再使用BAY 11-7082 处理12小时。细胞与[3H]胸甘温育3小时。使用自动细胞收集器将细胞收集到过滤器上,然后通过β-闪烁计数法测量放射性。


    (Only for Reference)

溶解度 (25°C)

体外 DMSO 41 mg/mL (197.82 mM)
Ethanol 10 mg/mL (48.25 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+corn oil
8mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 207.25
化学式

C10H9NO2S

CAS号 19542-67-7
储存条件 粉状
溶于溶剂
别名 BAY 11-7821

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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