Rimonabant (SR141716)

For research use only. Not for use in humans.

目录号:S3021 中文名称:利莫那班

Rimonabant (SR141716) Chemical Structure

CAS No. 168273-06-1

Rimonabant (SR141716)是一个大脑中心大麻素受体(CB1)特异性口服拮抗剂。在hCB1转染HEK293细胞中IC50浓度为13.6 nM,EC50浓度为17.3 nM。Rimonabant也是一种acyl CoA:cholesterol acyltransferases(ACAT) 12的双重抑制剂并且可抑制分支杆菌的MmpL3。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 990.19 现货
RMB 729.08 现货
RMB 1373.02 现货
RMB 2214.44 现货
RMB 3016.81 现货
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客户使用Selleck生产的Rimonabant (SR141716)发表文献4篇:

产品安全说明书

Cannabinoid Receptor抑制剂选择性比较

生物活性

产品描述 Rimonabant (SR141716)是一个大脑中心大麻素受体(CB1)特异性口服拮抗剂。在hCB1转染HEK293细胞中IC50浓度为13.6 nM,EC50浓度为17.3 nM。Rimonabant也是一种acyl CoA:cholesterol acyltransferases(ACAT) 12的双重抑制剂并且可抑制分支杆菌的MmpL3。
特性 有效诱导体重降低,并改善心血管代谢危险因素,然而由于引起严重的抑郁症和焦虑在2009年撤回。
靶点
ACAT1 [2]
()
ACAT2 [2]
()
MmpL3 [7]
()
hCB1 [1]
(Cell-free assay)
hCB2 [1]
(Cell-free assay)
13.6 nM 1.64 μM
体外研究

Rimonabant剂量依赖性降低Raw264.7巨噬细胞和离体腹膜巨噬细胞中ACAT活性,IC50为2.9 μM。Rimonabant以近似的效能抑制完整CHO-ACAT1和CHO-ACAT2细胞以及无细胞试验中ACAT活性,对CHO-ACAT1和 CHO-ACAT2的IC50分别为1.5 μM和2.2 μM。与ACAT抑制一致,Rimonabant治疗阻断巨噬细胞中ACAT相关过程,氧甾醇诱导的细胞凋亡和乙酰化LDL诱导的泡沫细胞形成。[2] Rimonabant显著减少细胞生长,并诱导人结肠直肠癌细胞(DLD-1,CaCo-2和SW620)的死亡。Rimonabant能够改变所有测试细胞系中细胞周期分布。特别是,Rimonabant引起DLD-1细胞中细胞周期阻滞,而不诱导细胞凋亡或坏死。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CHO cells M1jONGZ2dmO2aX;uJIF{e2G7 MknTRYJqdGm2eTD0c{BlcXOybHHj[UBcO0ifLWPSMUAyPDF5MU\BJIJqdmSrbnegeI8hcHWvYX6gR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubDDt[Y1jemGwZYOsJGtqRThwOTDuUS=> NVXXd4ZJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC0OlU2PTJpPkGwOFY2PTV{PD;hQi=>
CHO M4e0WmZ2dmO2aX;uJIF{e2G7 NV\LTIo3SW62YXfvcol{fGmlIHHjeIl3cXS7IITve4Fz\HNiY3HucoFjcW6xaXSgdoVk\XC2b4KgNUBmgHC{ZYPz[YQh[XNiW{PIYWFz[WOqaXTvcolkKGGlaXSgdoVt\WG|ZTDpckBEUE9iY3XscJMtKEumPUCuNFAzPTF{zszN MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDd|NkK0N{c,OTR5M{[yOFM9N2F-
CHO M3rQemZ2dmO2aX;uJIF{e2G7 NGPw[WVFcXOybHHj[Y1mdnRib3[gR3AuPTV7NECgZolv\GmwZzDmdo9uKHKnY3;tZolv[W62IHj1cYFvKGOjbn7hZolvd2mmIILlZ4VxfG:{IEGg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiS3m9NE4xOjYQvF2= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDd|NkK0N{c,OTR5M{[yOFM9N2F-
CHO NHvYTVlHfW6ldHnvckBie3OjeR?= NEDScJBC\m[rbnn0fUB1dyCmaYPwcIFk\SCFUD21OVk1OCCkaX7kbY5oKG[{b32gR4FvdmGkaX7vbYQhemWlZYD0c5IhOiCxZjDoeY1idiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzMEBMcT1zLkW4{txO NFXDXpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEezOlI1Oyd-MUS3N|YzPDN:L3G+
CHO NGHsenZHfW6ldHnvckBie3OjeR?= MVfJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCqdX3hckBz\WOxbXLpcoFvfCClYX7uZYJqdm:rZDDy[YNmeHSxcjD0fZBmKDFiZYjwdoV{e2WmIHnuJGNpcW6nc3WgTIFue3SncjDPeoFzgSBqQ1jPLUBk\WyuczygTWM2OD1yLkCwOu69VQ>? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTdzM{SwN{c,OTV5MUO0NFM9N2F-
CHO MnzrSpVv[3Srb36gZZN{[Xl? M{no[GlvKH[rdILvJINidm6jYnnuc4llKHKnY3XweI9zKDFiYX70ZYdwdmm|bTDv[kBcO0ifYYLhZ4hq\G:waXOgZYNq\CC{ZXzlZZNmKGK7IFPIU{Bk\WyuczygT4Q:OC5yMEK1NVLPxE1? MoP2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5N{G0NlgoRjF3N{exOFI5RC:jPh?=
CHO MVTGeY5kfGmxbjDhd5NigQ>? MWjJckB3cXS{bzDkbZNxdGGlZX3lcpQhd2ZiQ2CtOVU6PDBiYnnu[Ilv\yC2bzDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiS3m9NE4xOjYQvF2= M3v5NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{exOFI5Lz5zNUe3NVQzQDxxYU6=
CHO MXHGeY5kfGmxbjDhd5NigQ>? M{Tud2lvKH[rdILvJIRqe3CuYXPlcYVvfCCxZjDDVE02PTl2MDDibY5lcW6pIITvJIh2dWGwIFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPJINmdGy|LDDLbV0yNjV6zszN NI[3eGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe3NVQzQCd-MUW3O|E1Ojh:L3G+
CHO Mk[1SpVv[3Srb36gZZN{[Xl? M{XkTmlvcGmkaYTpc44hd2ZiW{PIYXNTNTF2MTy3NVZCKGKrbnTpcochfG9iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMtKEurPUCuNFA2PM7:TR?= NIqxUIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUiwNVg1OCd-MUW4NFE5PDB:L3G+
CHO MWTGeY5kfGmxbjDhd5NigQ>? NF\MZZFCdnSjZ3;ubZN1cWNiYXP0bZZqfHliYXfhbY5{fCClYX7uZYJqdm:rZDDy[YNmeHSxcjCxJI1m[XO3cnXkJIJ6KEOSLUW1PVQxKGmwZIXj[YQh[XKjY3juc4llKGGlaXSgdoVt\WG|ZTDpckBEUE9iY3XscJMtKEumPUCuNFAzPTF{zszN Ml3MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZzNECwNVAoRjF4MUSwNFExRC:jPh?=
CHO NGG2XmVHfW6ldHnvckBie3OjeR?= M4fZdGRqe3CuYXPlcYVvfCCxZjDzdIVkcW[rYzDDVE02PTl2MDDibY5lcW6pIHnuJGNJVyClZXzsd{B{fGGkbImgeJJidnOoZXP0[YQhf2m2aDDoeY1idiClYX7uZYJqdm:rZDDy[YNmeHSxcjCxMEBMcT1yLkCyOe69VQ>? NH3nd2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkG0NFAyOCd-MU[xOFAxOTB:L3G+
CHO MUnGeY5kfGmxbjDhd5NigQ>? NFXVXmNFcXOybHHj[Y1mdnRib3[gd5Bm[2moaXOgR3AuPTV7NECgZolv\GmwZzDpckBEUE9iY3XscJMhe3SjYnz5JJRz[W6|ZnXjeIVlKHerdHigbJVu[W5iY3HucoFjcW6xaXSgdoVk\XC2b4KgNkwhU2l;MT61PO69VQ>? M3TvU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4MUSwNFExLz5zNkG0NFAyODxxYU6=
CHOK1 M{XIbmZ2dmO2aX;uJIF{e2G7 MVPGeY5kfGmxbnHsJIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiC2cnHud4Zm[3SnZDDpckBEUE:NMTDj[YxteyCkeTDbN|VUT1SSXXfhcY1iWyCjc4PhfUwhU2l;MD6wNFHPxE1? MojYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ{NkOyPFMoRjF4Mk[zNlg{RC:jPh?=
HEK293 NUjW[pVwTnWwY4Tpc44h[XO|YYm= MXzEbZNxdGGlZX3lcpQhd2ZiW{PIYXNTNTF2MUexOkBnem:vIHj1cYFvKEOEMTDy[YNmeHSxcjD0doFve2[nY4Tl[EBqdiCKRVuyPVMh[2WubIOsJGtqRTBwMECyNe69VQ>? MknaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ{NkOyPFMoRjF4Mk[zNlg{RC:jPh?=
CHO NEjSXnJHfW6ldHnvckBie3OjeR?= MnKxSIl{eGyjY3Xt[Y51KG:oIGuzTH1UWjF2MUexOmEh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiS3m9NE4xODV2zszN M3z1[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4Mke5PFA6Lz5zNkK3PVgxQTxxYU6=
HEK293 M1LDOWZ2dmO2aX;uJIF{e2G7 NGfhOJpFcXOybHHj[Y1mdnRib3[gX|NJZVOULUG0NVcyPiCocn;tJIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBM\D1yLkCwNVjPxE1? M1H6O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEC0O|EzLz5zN{CwOFcyOjxxYU6=
HEK293 Ml3wSpVv[3Srb36gZZN{[Xl? M4f5TWRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygT4k:OC5yMEex{txO NWi3O4ZYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewNFQ4OTJpPkG3NFA1PzF{PD;hQi=>
HEK293 NU\J[ldXTnWwY4Tpc44h[XO|YYm= M4frSmRqe3CuYXPlcYVvfCCxZjDbN2heX0mQLUW1NlEzNTJiZoLvcUBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{whU2l;MD6wNVjPxE1? NVOySpV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewNFQ4OTJpPkG3NFA1PzF{PD;hQi=>
CHO MmfISpVv[3Srb36gZZN{[Xl? MkHkTY5pcWKrdHnvckBw\iCdM1jdR3AuPTV7NECgZolv\GmwZzD0c{BpfW2jbjDy[YNwdWKrbnHueEBESjFicnXj[ZB1d3JiaX6gR2hQKGOnbHzzMEBKSzVyPUCuNFA3Oc7:TR?= NH;CSYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G4NVE{QCd-MUexPFEyOzh:L3G+
CHO MonQSpVv[3Srb36gZZN{[Xl? M{HVbGlvcGmkaYTpc44hd2ZiW{PIYWNRNTV3OUSwJIJqdmSrbnegeI8hcHWvYX6gdoVkd22kaX7hcpQhS0J{IILlZ4VxfG:{IHnuJGNJVyClZXzsd{whUUN3ME2wMlbPxE1? NXy3VVF7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexPFEyOzhpPkG3NVgyOTN6PD;hQi=>
CHO MkHsSpVv[3Srb36gZZN{[Xl? NYDEO2luTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OVk1OCCocn;tJIh2dWGwIILlZ49u[mmwYX70JGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzMEBKSzVyPUCuNFA3Oc7:TR?= NV\nSodjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUeyPVMyODlpPkG3Nlk{OTB7PD;hQi=>
CHO NFT5b49HfW6ldHnvckBie3OjeR?= MX7EbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hemWlb33ibY5idnRiQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFnDOVA:OC54MEOz{txO NWqyfZA4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUeyPVMyODlpPkG3Nlk{OTB7PD;hQi=>
HEK293 MmS3SpVv[3Srb36gZZN{[Xl? MmD2SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0OFAh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygT4k:OC5yMEGx{txO MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN6M{G4NEc,OTd|OEOxPFA9N2F-
HEK293 MmHVSpVv[3Srb36gZZN{[Xl? NVO5VHBjTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OVk1OCCocn;tJIh2dWGwIILlZ49u[mmwYX70JGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{NCCNaU2wMlAyPs7:TR?= NYnSdo5QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5OFI{ODdpPkG3PVQzOzB5PD;hQi=>
HEK293 MnrFSpVv[3Srb36gZZN{[Xl? NFHx[IpFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMtKEurPUGuOlTPxE1? M330OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUSyN|A4Lz5zN{m0NlMxPzxxYU6=
HEK293 MmP4SpVv[3Srb36gZZN{[Xl? NXX5fGNWTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OVk1OCCocn;tJIh2dWGwIILlZ49u[mmwYX70JGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{NCCNaU2wMlA1P87:TR?= NGK4Tnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m3PVI3OSd-MUe5O|kzPjF:L3G+
CHOK1 NEDIZppHfW6ldHnvckBie3OjeR?= Mm\XSIl{eGyjY3Xt[Y51KG:oIGuzTH1YUU5vNUWyNVIzKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG9MOSClZXzsd{whU2l;MT65Pe69VQ>? NVjS[WVXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5O|kzPjFpPkG3PVc6OjZzPD;hQi=>
HEK293 NXLFWIRYTnWwY4Tpc44h[XO|YYm= NHK0VnVFcXOybHHj[Y1mdnRib3[gdoFlcW:uaXfhcoQh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygT4k:OC5yMEG4{txO MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB6M{W2NEc,OThyOEO1OlA9N2F-
HEK293 EBNA NHLzeY5HfW6ldHnvckBie3OjeR?= NWrqc29PUW64ZYLz[UBi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMOjl|IFXCUmEh[2WubIOgZpkhYzN3U23HWHBo[W2vYWOgbY5kd3Kyb4LheIlwdiCjc4PhfUwhTUN3ME2wMlAxPM7:TR?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB6M{W2NEc,OThyOEO1OlA9N2F-
HEK293 M{nK[WZ2dmO2aX;uJIF{e2G7 MoHlSIl{eGyjY3Xt[Y51KG:oIILh[IlwdGmpYX7kJIZzd21iaIXtZY4hS0J{IILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFvpQVAvPTV2zszN MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB6M{W2NEc,OThyOEO1OlA9N2F-
CHOK1 MXrGeY5kfGmxbjDhd5NigQ>? NXfCcIxpSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKT1uxJINmdGy|IHL5JIx2[2moZYLhd4Uh[XO|YYmsJGlEPTB;MD6xNu69VQ>? NHvkU3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0N|cyOSd-MUiyOFM4OTF:L3G+
CHOK1 Mor4SpVv[3Srb36gZZN{[Xl? M1H2bmRqe3CuYXPlcYVvfCCxZjDbN2heX0mQLUW1NlEzNTJiZoLvcUBpfW2jbjDDRlIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU2syKGOnbHzzMEBKSzVyPUGuO|bPxE1? Ml3sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEO3NVEoRjF6MkSzO|EyRC:jPh?=
HEK NVnYZZFUTnWwY4Tpc44h[XO|YYm= NGnQTVZFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWsh[2WubIOsJGtqRTBwMEGy{txO MoTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{OUO5NFgoRjF6MkmzPVA5RC:jPh?=
HEK MV3GeY5kfGmxbjDhd5NigQ>? MWPEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hemWlb33ibY5idnRiQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVugZ4VtdHNuIFvpQVAvPzoQvF2= MlWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{OUO5NFgoRjF6MkmzPVA5RC:jPh?=
HEK293 NVzjXldKTnWwY4Tpc44h[XO|YYm= NGT2VnpFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5iQ1KxVkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBMcT1yLkCwOu69VQ>? MlHhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|M{W5O|YoRjF6M{O1PVc3RC:jPh?=
CHO MWLGeY5kfGmxbjDhd5NigQ>? NVvndmZITGm|cHzhZ4Vu\W62IH;mJHs{UF2ZSV6tOVUzOTJvMjDmdo9uKGi3bXHuJINidm6jYnnuc4llKEOEMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCLQ{WwQVEvPzcQvF2= NX[0S3BKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizN|cxQTZpPkG4N|M4ODl4PD;hQi=>
CHO NGXsPFJHfW6ldHnvckBie3OjeR?= Ml7RSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCNaU2wMlAzPc7:TR?= MofoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|NEK0NFMoRjF6M{SyOFA{RC:jPh?=
CHO MWXGeY5kfGmxbjDhd5NigQ>? NYOxOXlwTGm|cHzhZ4Vu\W62IH;mJHs{UF2ZSV6tOVUzOTJvMjDmdo9uKGi3bXHuJGNDOiC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzMEBMcT1zLkW4{txO NV7p[41HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizOFI1ODNpPkG4N|QzPDB|PD;hQi=>
HEK293 M4L2VGZ2dmO2aX;uJIF{e2G7 MYrEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFvpQVAvODB{NN88US=> MlrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|NkOzOVIoRjF6M{[zN|UzRC:jPh?=
CHO NFXrWYVHfW6ldHnvckBie3OjeR?= NFfCOYNFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5iQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNuIFvpQVAvPTcQvF2= M2\GblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{[zN|UzLz5zOEO2N|M2OjxxYU6=
SF9 MkfTSpVv[3Srb36gZZN{[Xl? NGTxcJBCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IILheEBESjFicnXj[ZB1d3JiaX6gV2Y6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gR3AuPTV7NECtd5RqdXWuYYTl[EBIXFCpYX3tZXMh[mmwZHnu[{whU2l;MD6wNFA{Oc7:TR?= M2jyT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NES4N|QxLz5zOES0PFM1ODxxYU6=
SF9 Ml3XSpVv[3Srb36gZZN{[Xl? Mo\ZRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJHNHQSClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGNRNTV3OUSwMZN1cW23bHH0[YQhT1SSZ3HtcYFUKGKrbnTpcoctKEurPUCuNFAxPDQQvF2= NHPxXXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES0PFM1OCd-MUi0OFg{PDB:L3G+
SF9 NVjFOJpZTnWwY4Tpc44h[XO|YYm= NF3LUYRKdn[ncoPlJIFod26rc4SgZZQhcHWvYX6gR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDTSlkh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iR2TQ[4FudWGVIHzleoVtNCCLQ{WwQVAvODBzM{ZOwG0> MlLFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2NEizOFAoRjF6NES4N|QxRC:jPh?=
SF9 MmPuSpVv[3Srb36gZZN{[Xl? NUnv[ohDSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFQkKgdoVk\XC2b4KgbY4hW0Z7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1BvNUW5OFAue3SrbYXsZZRm\CCJVGDnZY1u[VNiYnnu[Ilv\yxiS3m9NE45OTYQvF2= NXK1[YNpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0OFg{PDBpPkG4OFQ5OzRyPD;hQi=>
HEK293 M4DxXWZ2dmO2aX;uJIF{e2G7 NEDINm9FcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[xcn2gbJVu[W5icnXjc41jcW6jboSgR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[nlibHnxeYllKHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtqRTBwMEVOwG0> Mlz4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3MUGxOVcoRjF6NUGxNVU4RC:jPh?=
CHO-K1 NUDGWmNQTnWwY4Tpc44h[XO|YYm= MoX5SIl{eGyjY3Xt[Y51KG:oIGuzTH1UWjF2MUexOkBnem:vIHj1cYFvKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRLVuxJINmdGy|LDDLbV0xNjByMUG4{txO M3THW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGyPVAyLz5zOEWxNlkxOTxxYU6=
HEK293 NH3SPYhHfW6ldHnvckBie3OjeR?= MlPLRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEOSLUW1PVQxNXO2aX31cIF1\WRiW{O1V31IXFCpYX3tZXMh[mmwZHnu[{whU2R;MD6wNFI2P87:TR?= M1jOUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUGyPVAyLz5zOEWxNlkxOTxxYU6=
HEK293 NVzMNnhxTnWwY4Tpc44h[XO|YYm= MV7EbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFvpQVAvODB4MUlOwG0> NYPFUlByRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi1NVI6ODFpPkG4OVEzQTBzPD;hQi=>
CHO-K1 MUjGeY5kfGmxbjDhd5NigQ>? M1;5S2Rqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiCFQkKgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTz3LNUBk\WyuczygT4k:OC5|MUROwG0> Mlj1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3MUK5NFEoRjF6NUGyPVAyRC:jPh?=
HEK MlXDSpVv[3Srb36gZZN{[Xl? NVjCSYRyTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OVk1OCCocn;tJIh2dWGwIILlZ49u[mmwYX70JGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gTGVMKGOnbHzzMEBMcT1yLkCwPO69VQ>? MnLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3N{mzPFYoRjF6NUe5N|g3RC:jPh?=
HEK Mn3OSpVv[3Srb36gZZN{[Xl? NIHrU|FFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWsh[2WubIOsJGtqRTBwN{pOwG0> MkHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3N{mzPFYoRjF6NUe5N|g3RC:jPh?=
HEK293 NILISHRHfW6ldHnvckBie3OjeR?= NWTzd5MxTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OVk1OCCocn;tJIh2dWGwIFPCNUBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBMcT1yLkCxNu69VQ>? NUjoUYFjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2PFAzPzZpPkG4OlgxOjd4PD;hQi=>
HEK293 MUPGeY5kfGmxbjDhd5NigQ>? NVTQboRzTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OVk1OCCocn;tJIh2dWGwIFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBMcT1yLke5{txO NHT1ToE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[4NFI4Pid-MUi2PFAzPzZ:L3G+
HEK293 M1O1O2Z2dmO2aX;uJIF{e2G7 MWDEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFnDOVA:OC5yMUZOwG0> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDdzMki1Okc,OTh5MUK4OVY9N2F-
HEK293 NV3ofIdyTnWwY4Tpc44h[XO|YYm= M1mxcWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCHdT3HWHAh[mmwZHnu[{whTUN3ME2wMlAyQDMQvF2= MkDiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh5MUK4OVYoRjF6N{GyPFU3RC:jPh?=
HEK293 NGP4RVhHfW6ldHnvckBie3OjeR?= NV\IR|NtTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OVk1OCCocn;tJIh2dWGwIFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBKSzVyPUGuPVM6QM7:TR?= NEDi[VQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEexNlg2Pid-MUi3NVI5PTZ:L3G+
HEK293 NX;jdHhsTnWwY4Tpc44h[XO|YYm= MWPJcpZmenOnIHHnc45qe3RiYXP0bZZqfHliYYSgbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMh\GWlcnXhd4UhcW5iW{O1V31o[W2vYVfUVEBjcW6maX7nJJJmdGG2aY\lJJRwKGOxboTyc4w> NEnVcnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEexNlg2Pid-MUi3NVI5PTZ:L3G+
CHO M1PZd2Z2dmO2aX;uJIF{e2G7 NEHTOmlCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHj1cYFvKEOEMTDy[YNmeHSxcjDpckBEUE9iY3XscJMh[XO|ZYPz[YQh[XNiR2TQ[4FudWGVIHLpcoRqdmduIFviQVAvODByNkm4{txO M3G1TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6OECwO|cxLz5zOEiwNFc4ODxxYU6=
CHOK1 MoPRSpVv[3Srb36gZZN{[Xl? M4PRfmRqe3CuYXPlcYVvfCCxZjDbN2heX0mQLUW1NlEzNTJiZoLvcUBpfW2jbjDDRlIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU2syKGOnbHzzJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIIPw[YN1em:vZYTyfUwhUUN3ME2xMlc3|ryP MmrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh7NUSwOFIoRjF6OUW0NFQzRC:jPh?=
CHO M2juTmZ2dmO2aX;uJIF{e2G7 NFjpZWZFcXOybHHj[Y1mdnRib3[gX|NJZVeLTj21OVIyOi1{IH\yc40hcHWvYX6gR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOsJGlEPTB;MT63Ou69VQ>? NUnkc4NvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwNlI3PjZpPkG5NFIzPjZ4PD;hQi=>
HEK293 NEnY[3RHfW6ldHnvckBie3OjeR?= M33CVGRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiCFQkKgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygTWM2OD1yLkCwNVnPxE1? M3;nXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEm1OFQ1Lz5zOUC5OVQ1PDxxYU6=
HEK293 NFnYb|lHfW6ldHnvckBie3OjeR?= Mm\GSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|LDDJR|UxRTBwMEG1{txO NXz4RpZiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwPVU1PDRpPkG5NFk2PDR2PD;hQi=>
HEK293 NYj3[3c1TnWwY4Tpc44h[XO|YYm= MoDpRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEW3LVfUVEBjcW6maX7nMEBGSzVyPUCuNFE5Os7:TR?= NYT6WmF[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwPVU1PDRpPkG5NFk2PDR2PD;hQi=>
CHO-K1 MW\GeY5kfGmxbjDhd5NigQ>? NIjYbJVCdnSjZ3;ubZN1KGGldHn2bZR6KGGpYXnud5QhcHWvYX6gR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8uUzFiY3XscJMh[nliW{O1V31IXFCpYX3tZUBjcW6maX7nJIF{e2G7LDDLbV0xNjByMEG5{txO NUDaS25uRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNFI3QThpPkG5NVAzPjl6PD;hQi=>
HEK293 M1zNbWZ2dmO2aX;uJIF{e2G7 M3O3W2Rqe3CuYXPlcYVvfCCxZjDbN2heW1JzNEG3NVZCKG[{b32gbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOsJGtqRTBwMECwPe69VQ>? NVjxZnVxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxNFI3QThpPkG5NVAzPjl6PD;hQi=>
CHOK1 MULGeY5kfGmxbjDhd5NigQ>? NFfNTWlFcXOybHHj[Y1mdnRib3[gX|NJZVeLTj21OVIyOi1{IH\yc40hcHWvYX6gdoVkd22kaX7hcpQh[2GwbnHibY5wcWRiQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF;LNUBk\WyuczDifUBtcXG3aXSgd4NqdnSrbHzheIlwdiC|cHXjeJJwdWW2comsJGlEPTB;MT63Ou69VQ>? MlP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl{Nkm4NVcoRjF7Mk[5PFE4RC:jPh?=
CHOK1 M1LKdWZ2dmO2aX;uJIF{e2G7 MYfBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJINidm6jYnnuc4llKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRS{GgZ4VtdHNiYYPz[ZN{\WRiYYOgZ2FOWCCjY4Tpeol1gSxiRVO1NF0xNjJ2zszN MonHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|Mki2PFMoRjF7M{K4Olg{RC:jPh?=
CHOK1 NWDYfGtxTnWwY4Tpc44h[XO|YYm= NWPWbHVJSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiClYX7uZYJqdm:rZDDDRlIhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU2syKGOnbHzzJIF{e2W|c3XkJIF{KGODTWCgZYN1cX[rdImsJGVEPTB;M{GuNlHPxE1? M1zN[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{K4Olg{Lz5zOUOyPFY5OzxxYU6=
HEK293 MmDVSpVv[3Srb36gZZN{[Xl? MVHEbZNxdGGlZX3lcpQhd2ZiW{PIYXNTOTRzN{G2JIZzd21iaIXtZY4hemWlb33ibY5idnRiQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOsJGtqRTBwMECxPVk2|ryP MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN|OEO1Okc,OTl|M{izOVY9N2F-
HEK293 MoHLSpVv[3Srb36gZZN{[Xl? Ml7USIl{eGyjY3Xt[Y51KG:oIGuzTH1UWjF2MUexOkBnem:vIHj1cYFvKHKnY3;tZolv[W62IFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBMcT1yLkO5PFEy|ryP MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN|OEO1Okc,OTl|M{izOVY9N2F-
CHOK1 MY\GeY5kfGmxbjDhd5NigQ>? MVKxNEBucW6| MkTIRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJV0tzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1BvNUW5OFAucW6mdXPl[EBz\XOyb37z[UBi\nSncjCxNEBucW6|IHL5JGdVWGejbX3hX|M2W11iYnnu[Ilv\yCjc4PhfUwhU2l;MD6wNFE3|ryP MoruQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NUGxNVMoRjF7M{WxNVE{RC:jPh?=
HEK293 MU\GeY5kfGmxbjDhd5NigQ>? NHrVd2lFcXOybHHj[Y1mdnRib3[gX|NJZXKrbX;uZYJidnRiZoLvcUBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{BjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4k:OC5yMEG5{txO M{Hsd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUKwOVczLz5zOUWyNFU4OjxxYU6=
HEK293 NX3jNXRPTnWwY4Tpc44h[XO|YYm= NFrmdnFFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IyWiCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{NCCLQ{WwQVAvODF|Mt88US=> NFW2SHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWzNFY6Pyd-MUm1N|A3QTd:L3G+
HEK293 NWXOdJhTTnWwY4Tpc44h[XO|YYm= NU\DO|VbUW64ZYLz[UBi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJJJm[2:vYnnuZY51KEOEMWKg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFPQMVU2QTRyLYP0bY12dGG2ZXSgSZUuT1SSIHLpcoRqdmduIFXDOVA:OC5yMUW3{txO NHPTVWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWzNFY6Pyd-MUm1N|A3QTd:L3G+
HEK293 M2e4WGZ2dmO2aX;uJIF{e2G7 MWXEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hemWlb33ibY5idnRiQ1KyVkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzMEBKSzVyPUGuOlMyOc7:TR?= NWjoUIZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1N|A3QTdpPkG5OVMxPjl5PD;hQi=>
HEK293 Ml3XSpVv[3Srb36gZZN{[Xl? M{e5NFExKHWP NXzCVlJ1UW62cnnud4lkKGGldHn2bZR6KGG2IHj1cYFvKHKnY3;tZolv[W62IFPCNXIh\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bie3Onc4Pl[EBieyCHdT3HWHAh[mmwZHnu[{BifCBzMDD1UUBz\WyjdHn2[UB1dyCkYYPhcEBt\X[nbB?= NYj6WFU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1N|A3QTdpPkG5OVMxPjl5PD;hQi=>
HEK MnzBSpVv[3Srb36gZZN{[Xl? NGXT[HZFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWsh[2WubIOsJGtqRTBwMEGy{txO MkjqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3OUW1PVYoRjF7NUm1OVk3RC:jPh?=
HEK MXjGeY5kfGmxbjDhd5NigQ>? NFK3SGVFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWsh[2WubIOsJGtqRTBwN{pOwG0> NHXzVIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUW5OVU6Pid-MUm1PVU2QTZ:L3G+
CHO-K1 NEm2SZhHfW6ldHnvckBie3OjeR?= MV7BcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiC2cnHud4Zm[3SnZDDpckBEUE9vS{Hj[YxteyCkeTDHWHBo[W2vYWuzOXNeKGKrbnTpcoch[XO|YYmsJGtqRTBwMECxOu69VQ>? MnH4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl4OEO5NVgoRjF7NkizPVE5RC:jPh?=
CHO NVrpcnpITnWwY4Tpc44h[XO|YYm= NHfEOVZDcW6maX7nJIFn\mmwaYT5JJRwKGi3bXHuJGNDOiC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkC5NlXPxE1? MljNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6NUC0O|MoRjF7OEWwOFc{RC:jPh?=
CHOK1 NFHvTlFHfW6ldHnvckBie3OjeR?= NUnqSIh3TGm|cHzhZ4Vu\W62IH;mJHs{UF2ZSV6tOVUzOTJ{IH\yc40hcHWvYX6gR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG9MOSClZXzsd{whUUN3ME2xMlc3|ryP NU\0SYVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4OVA1PzNpPkG5PFUxPDd|PD;hQi=>
CHO-K1 M{S5bGZ2dmO2aX;uJIF{e2G7 M3\DfmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hemWlb33ibY5idnRiQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:tT|Eh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDDVE02PTl2MD3zeIlufWyjdHXkJGdVWGejbX3hV{BjcW6maX7nMEBKSzVyPUCuNFA1Pc7:TR?= NVnDe|hURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OVQ6PzhpPkG5PVU1QTd6PD;hQi=>
CHO-K1 NXTkPZVpTnWwY4Tpc44h[XO|YYm= M2G4RmlvfmW{c3WgZYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{1MOSClZXzsd{BjgSCJVGDnZY1u[VNiYnnu[Ilv\yCjc4PhfUwhUUN3ME2wMlAxOjoQvF2= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODBzNU[0O{c,OjByMUW2OFc9N2F-
CHO-K1 MkPkSpVv[3Srb36gZZN{[Xl? MWnBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQNUtzIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhS1BvNUW5OFAucW6mdXPl[EBIXFCpYX3tZXMh[mmwZHnu[{whUUN3ME2wMlAxPDYQvF2= MmnzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByMUW2OFcoRjJyMEG1OlQ4RC:jPh?=
COS7 MYDGeY5kfGmxbjDhd5NigQ>? MnvXSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0:VNzDj[YxteyxiSVO1NF0xNjByNUJOwG0> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODBzNU[0O{c,OjByMUW2OFc9N2F-
CHO NUHtbmQzTnWwY4Tpc44h[XO|YYm= MnXuTY53\XK|ZTDh[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIILlZ49u[mmwYX70JGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1yLkGwPO69VQ>? MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB2NUOzO{c,OjByNEWzN|c9N2F-
CHO MnrlSpVv[3Srb36gZZN{[Xl? Mo\TOlAhdWmwcx?= MWLJcpZmenOnIHHnc45qe3RiYXP0bZZqfHliYYSgbJVu[W5iY3HucoFjcW6xaXSgR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDmc5J{c2:uaX6td5RqdXWuYYTl[EBkSU2SIHHjZ5VufWyjdHnvckBi\nSncjC2NEBucW6|LDDFR|UxRTBwMECwNVHPxE1? M2jaZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMES3O|c6Lz5{MEC0O|c4QTxxYU6=
CHO MXnGeY5kfGmxbjDhd5NigQ>? M3LBWFMhcHK| Mn33SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKHKnY3;tZolv[W62IHPhco5i[mmwb3nkJGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIFnfGW{IEOgbJJ{KGK7IHzpdZVq\CC|Y3nueIltdGG2aX;uJINwfW62aX7nMEBMcT1yLkCwNFc1|ryP MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB2N{e3PUc,OjByNEe3O|k9N2F-
CHO NXT5VGRFTnWwY4Tpc44h[XO|YYm= M3nnZ|ExKG2rboO= NVu0Z4VDSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiClYX7uZYJqdm:rZDDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDjc4V5eHKnc4PpcochT2GucHjhNVUwOTZiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCFUD21OVk1OC2rbnT1Z4VlKEOjMjugdoVt\WG|ZTDh[pRmeiBzMDDtbY5{KGK7IH3pZ5JwKHCuYYTlJJJm[WSncjygTWM2OD1yLkCwN|LPxE1? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB2N{e3PUc,OjByNEe3O|k9N2F-
CHO MWfGeY5kfGmxbjDhd5NigQ>? NVTTeVhGOyCqcoO= M2TwRmRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDjZY5v[WKrbn;p[EBESjJicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{Bi\nSncjCzJIhzeyCkeTDsbZF2cWRic3PpcpRqdGyjdHnvckBkd3WwdHnu[{whU2l;MD6xNlbPxE1? NHPDPYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC0O|c4QSd-MkCwOFc4Pzl:L3G+
CHO NELwNXdHfW6ldHnvckBie3OjeR?= MlWxRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGNRNTV3OUSwMYlv\HWlZXSgX|NJZWG{YXPobYRwdmmlIHHjbYQhemWuZXHz[UwhU2R;MD6wNFI2OTMQvF2= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODN4M{GzNkc,OjB|NkOxN|I9N2F-
CHO MX7GeY5kfGmxbjDhd5NigQ>? MVrEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMtKEurPUCuNFI2|ryP NVHLZ|c4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzOlMyOzJpPkKwN|Y{OTN{PD;hQi=>
CHO MoK1SpVv[3Srb36gZZN{[Xl? Mny4SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKEOEMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{NCCNaU2xMlU5|ryP MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODN4M{GzNkc,OjB|NkOxN|I9N2F-
CHO-K1 NYLFUFNVTnWwY4Tpc44h[XO|YYm= MVixJIhz MXTEbZNxdGGlZX3lcpQhd2ZiW{PIYXdKVi13NUKxNk0zKG[{b32gbJVu[W5iQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:tT|Eh[2WubIOgZYZ1\XJiMTDodkBjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygTWM2OD1zLke2{txO NX;sWnBQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2O|M4OjlpPkKwOlc{PzJ7PD;hQi=>
HEK M2mxTmZ2dmO2aX;uJIF{e2G7 M{n0cWRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHSzDj[YxteyxiS3m9NE4xOTMQvF2= NVrwTohNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC3NVg1QTJpPkKwO|E5PDl{PD;hQi=>
HEK NHfSeWZHfW6ldHnvckBie3OjeR?= NHq2dYdFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1PVQxKG[{b32gbJVu[W5iQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVugZ4VtdHNuIFvpQVAvPzoQvF2= Ml3lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB5MUi0PVIoRjJyN{G4OFkzRC:jPh?=
CHO MX;GeY5kfGmxbjDhd5NigQ>? M1LsO2Fod26rc4SgZYN1cX[rdImgZZQhcHWvYX6gR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZ48u\XiycnXzd4lv\yCJYXzwbIEyPiCycn;0[YlvKGG|c3Xzd4VlKGG|IH3vZoltcXqjdHnvckBw\iCrboTyZYNmdGy3bHHyJINidGOrdX2sJGtmRTBwMECxNe69VQ>? M3L6OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOES1PVU6Lz5{MEi0OVk2QTxxYU6=
HEK293 NF;rfFNHfW6ldHnvckBie3OjeR?= MULEbZNxdGGlZX3lcpQhd2ZiW{PIYXNTOTRzN{G2JIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNuIFvpQVAvODBzMUlOwG0> M1rhd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOES1PVU6Lz5{MEi0OVk2QTxxYU6=
HEK293 M{XZcWZ2dmO2aX;uJIF{e2G7 NIrmWFZFcXOybHHj[Y1mdnRib3[gX|NJZUOSNUW5OFAh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczygT4k:OC5yME[xPO69VQ>? M3XScFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOES1PVU6Lz5{MEi0OVk2QTxxYU6=
CHOK1 MkHaSpVv[3Srb36gZZN{[Xl? MUPEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRPTV7NECg[pJwdSCqdX3hckBESjJicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJV0tzIHPlcIx{NCCNaU2wMlMyO87:TR?= NVPibGNYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4OFU6PTlpPkKwPFQ2QTV7PD;hQi=>
HEK NUPNe2VGTnWwY4Tpc44h[XO|YYm= NInHeYxFcXOybHHj[Y1mdnRib3[gX|NJZUOSLUW1MFk1OCCocn;tJJJm[2:vYnnuZY51KGi3bXHuJGNDOSC{ZXPldJRweiC2cnHud4Zm[3SnZDDpckBJTUtiY3XscJMtKEurPUCuNFEz|ryP NF\rc489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEm0N|I6OCd-MkC5OFMzQTB:L3G+
HEK NHS1[XVHfW6ldHnvckBie3OjeR?= NWrlWI9iTGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OUw6PDBiZoLvcUBz\WOxbXLpcoFvfCCqdX3hckBESjJicnXj[ZB1d3JidILhcpNn\WO2ZXSgbY4hUEWNIHPlcIx{NCCNaU2wMlc6|ryP M1T4WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOUSzNlkxLz5{MEm0N|I6ODxxYU6=
CHO M2fIZmZ2dmO2aX;uJIF{e2G7 MoK0SIl{eGyjY3Xt[Y51KG:oIGuzTH1UWjF2MUexOmEh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyxiS3m9NE4xODB7M{Oz{txO Ml3PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{S4PVIoRjJzM{O0PFkzRC:jPh?=
Sf9 M2fMUmZ2dmO2aX;uJIF{e2G7 M{PnNGRqe3CuYXPlcYVvfCCxZjDbN2heS1B3NUm0NEBnem:vIHj1cYFvKHKnY3;tZolv[W62IFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|LDDLbV0xNjh3MUG0{txO MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|NEi5Nkc,OjF|M{S4PVI9N2F-
CHO M3XyWmZ2dmO2aX;uJIF{e2G7 MnzrRY51[WexbnnzeEBi[3Srdnn0fUBifCCFQkGgdoVk\XC2b4KgeJJidnOoZXP0[YQhcW5iQ1jPJINmdGy|IHX4dJJme3OrbnegZZBw[WWzdX;ybY4h[XNiYTDy[ZBwenSncjDmc5IhTy2ycn;0[YlvNWOxdYDs[YQhemWlZYD0c5IudWWmaXH0[YQh[2GuY3n1cUB{cWewYXzpcoch[nliYnnvcJVucW6nc3PlcoNmKGG|c3H5MEBKSzVyPUCuNFA1|ryP NHn6PZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO3OlU5QCd-MkGzO|Y2QDh:L3G+
HEK293 NWm4W3F[TnWwY4Tpc44h[XO|YYm= MoDEUo9v[2:vcHX0bZRqfmViaX7obYJqfGmxbjDv[kBOWlBzLX3l[IlifGWmIFWyMVE4[mW2YVegeJJidnOyb4L0JIlvKGi3bXHuJG1TWDFiZYjwdoV{e2mwZzDISWszQTNiY3XscJMh[nliRHn4c44heGyxdDygT4k:OS52zszN MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzMUm0OUc,OjF3MUG5OFU9N2F-
HEK293 NGK0R4pHfW6ldHnvckBie3OjeR?= NHXD[oVPd26lb33w[ZRqfGm4ZTDpcohq[mm2aX;uJI9nKE2UUEStcYVlcWG2ZXSgSVIuOTekZYThS{B1emGwc4DvdpQhcW5iaIXtZY4hVVKSNDDlfJBz\XO|aX7nJGhGUzJ7MzDj[YxteyCkeTDEbZhwdiCybH;0MEBMcT12zszN NVv5ToRSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVE6PDVpPkKxOVEyQTR3PD;hQi=>
HEK293 NI\F[3FHfW6ldHnvckBie3OjeR?= NWnyfWRPUW6mdXP0bY9vKG:oIF3SVFIudWWmaXH0[YQhTTJvMUfi[ZRiTyC2cnHud5BwenRiaX6gbJVu[W5iTWLQNkBmgHC{ZYPzbY5oKEiHS{K5N{Bk\WyuczDpckBxemW|ZX7j[UBw\iB{IIXNJGUzNTF5YnX0ZWc> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzMUm0OUc,OjF3MUG5OFU9N2F-
HEK293 MWHGeY5kfGmxbjDhd5NigQ>? NFXaeJVKdmirYnn0bY9vKG:oIF3SVFMudWWmaXH0[YQhTTJvMUfi[ZRiTyC2cnHud5BwenRiaX6gbJVu[W5iTWLQN{BmgHC{ZYPzbY5oKEiHS{K5N{Bk\WyuczDpckBxemW|ZX7j[UBw\iBzNTD1UUBGOi1zN3LleIFI MmHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MUG5OFUoRjJzNUGxPVQ2RC:jPh?=
HEK293 NHPEeJlHfW6ldHnvckBie3OjeR?= M13nZWlvcGmkaYTpc44hd2ZiTWLQNU1u\WSrYYTl[EBGOi1zN3LleIFIKHS{YX7zdI9zfCCrbjDoeY1idiCPUmCxJIV5eHKnc4PpcochUEWNMkmzJINmdGy|IHnuJJBz\XOnbnPlJI9nKDBwNjD0c{A2KHWPIFWyMVE4[mW2YVe= NYXuNmxRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NVE6PDVpPkKxOVEyQTR3PD;hQi=>
HEK293 NEj5WnlHfW6ldHnvckBie3OjeR?= NIrHU4dKdmirYnn0bY9vKG:oIF3SVFQudWWmaXH0[YQhTTJvMUfi[ZRiTyC2cnHud5BwenRiaX6gbJVu[W5iTWLQOEBmgHC{ZYPzbY5oKEiHS{K5N{Bk\WyuczDpckBxemW|ZX7j[UBw\iByLk[geI8hPSC3TTDFNk0yP2KndHHH MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVzMUm0OUc,OjF3MUG5OFU9N2F-
HEK MkTtSpVv[3Srb36gZZN{[Xl? M2P4W2Rqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT{Bk\WyuczygT4k:OC5yMUNOwG0> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdyMkS5PEc,OjF5MEK0PVg9N2F-
HEK MmHCSpVv[3Srb36gZZN{[Xl? MV\EbZNxdGGlZX3lcpQhd2ZiW{PIYWNRNTV3OUSwJIZzd21iaIXtZY4hemWlb33ibY5idnRiQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVugZ4VtdHNuIFvpQVAvPzoQvF2= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdyMkS5PEc,OjF5MEK0PVg9N2F-
CHO NGf5SldHfW6ldHnvckBie3OjeR?= M4P3RVEhcHJ? MkPWSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3OUSwJIZzd21iaIXtZY4h[2GwbnHibY5wcWRiQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYYSgdGghPy52IHHmeIVzKDFiaIKgZpkhdGmzdXnkJJNkcW62aXzsZZRqd25iY3;1cpRqdmduIFnDOVA:OS57ON88US=> NUHMdY5FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3OFE5OzVpPkKxO|QyQDN3PD;hQi=>
HEK293 NV3vUZlITnWwY4Tpc44h[XO|YYm= M2fN[FUhdWmwcx?= MULJcohq[mm2aX;uJI9nKGi3bXHuJGVTTyCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YheG:2YYPzbZVuKGOqYX7u[Ywh[3W{cnXueEBi\nSncjC1JI1qdnNiYomgdIF1[2hiY3zhcZAh[XO|YYmsJGlEPTB;Mj63Pe69VQ>? NGnvZZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUe0NVg{PSd-MkG3OFE5OzV:L3G+
CHO NU\nZ5ZHTnWwY4Tpc44h[XO|YYm= NYTXWG5tQTBibXnudy=> MXvEbZNxdGGlZX3lcpQhd2ZiW{PIYWJOWy15MkW1NVkh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyCjZoTldkA6OCCvaX7zJIJ6KHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtqRTBwMECwO|PPxE1? Mn20QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NkK1O|UoRjJzOU[yOVc2RC:jPh?=
CHO-K1 NFfafJFHfW6ldHnvckBie3OjeR?= MmjWRY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJVy2NMTDj[YxteyClbz3lfJBz\XO|aX7nJGdidHCqYYGxOkBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGNRPTV7NECtbY5lfWOnZDDpcpRz[WOnbHz1cIFzKGOjbHPpeY0hdW:kaXzpfoF1cW:wIHL5JIZtfW:{b33leJJ6NCCNZU2wMlAxOTIQvF2= NVf0WpVbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzO|I5OzVpPkKyN|czQDN3PD;hQi=>
CHO-K1 MlfESpVv[3Srb36gZZN{[Xl? NIizXXRFcXOybHHj[Y1mdnRib3[gX|NJZUOSNUW5OFAh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTz3LNUBk\WyuczygT4k:OC5yME[y{txO M3z5bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{eyPFM2Lz5{MkO3Nlg{PTxxYU6=
CHO-K1 M2T6O2Z2dmO2aX;uJIF{e2G7 M2rCWmRqe3CuYXPlcYVvfCCxZjDbN2heS1B3NUm0NEBnem:vIFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPMWsyKGOnbHzzMEBMcT1yLkOxN:69VQ>? MoLYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ|N{K4N|UoRjJ{M{eyPFM2RC:jPh?=
HEK NEjT[lFHfW6ldHnvckBie3OjeR?= NWLHfmJ5TGm|cHzhZ4Vu\W62IH;mJHs{UF2FUD21OUw6PDBiZoLvcUBpfW2jbjDy[YNwdWKrbnHueEBESjFicnXj[ZB1d3JidILhcpNn\WO2ZXSgbY4hUEWNIHPlcIx{NCCNaU2wMlAyOs7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2OES1O{c,OjJ3NEi0OVc9N2F-
HEK MnjpSpVv[3Srb36gZZN{[Xl? M1vJbGRqe3CuYXPlcYVvfCCxZjDbN2heS1BvNUWsPVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgR2IzKHKnY3XweI9zKHS{YX7z[oVkfGWmIHnuJGhGUyClZXzsd{whU2l;MD63Pe69VQ>? MnG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NEi0OVcoRjJ{NUS4OFU4RC:jPh?=
CHO NEPSU5VHfW6ldHnvckBie3OjeR?= M2f3PVIhcHK| MnjlSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3LEm0NEBnem:vIHj1cYFvKEOEMTDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRIHPlcIx{KGGodHXyJFIhcHK|IHL5JIxqeXWrZDDzZ4lvfGmubHH0bY9vKGOxdX70[ZItKEurPUCuNFEzPs7:TR?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjlzNkewO{c,OjJ7MU[3NFc9N2F-
CHO M{TYdmZ2dmO2aX;uJIF{e2G7 NXflTmlXOiCqcoO= NF;5PWhFcXOybHHj[Y1mdnRib3[gX|NJZUOSNUWsPVQxKG[{b32gbJVu[W5iQ1KyJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYX\0[ZIhOiCqcoOgZpkhdGmzdXnkJJNkcW62aXzsZZRqd25iY3;1cpRmeixiS3m9NE46|ryP Mm\DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7MU[3NFcoRjJ{OUG2O|A4RC:jPh?=
CHO MYTGeY5kfGmxbjDhd5NigQ>? NWfM[|Q2UW64ZYLz[UBi\2:waYP0JIFkfGm4aYT5JIFo[Wmwc4SgR2IyKHKnY3XweI9zKGW6cILld5Nm\CCrbjDoeY1idiCFSF:gZ4VtdHNiYYPz[ZN{\WRiYYOg[YZn\WO2IH;uJIZwenOtb3zpck1{fGmvdXzheIVlKGODTWCgcIV3\WxuIFnDOVA:OC5yNUJOwG0> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl3OUK0PUc,OjJ7NUmyOFk9N2F-
CHO MoPGSpVv[3Srb36gZZN{[Xl? NWH1RY8yOSCqch?= Mmj4SIl{eGyjY3Xt[Y51KG:oIGuzTH1EWDV3OUSwJIZzd21iaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMhcW6ldXLheIVlKG[xcjCxJIhzNCCNaU2wMlAyODcQvF2= M{LaV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEeyN|M6Lz5{M{C3NlM{QTxxYU6=
CHO M{jYfGZ2dmO2aX;uJIF{e2G7 M17FVl4hOC5zIH7N NEnIe20yKGi{ MoTPTY53\XK|ZTDh[49vcXO2IHHjeIl3cXS7IHH0JIh2dWGwIILlZ49u[mmwYX70JGNDOSC{ZXPldJRweiC2cnHud4Zm[3SnZDDpckBEUE9iY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJHs{PVOfR2TQ[4FudWGVIHLpcoRqdmdiYYSgQkAxNjFibl2gZYZ1\XJiMTDodkBjgSCuaYH1bYQhe2OrboTpcIxifGmxbjDzdIVkfHKxbXX0dpk> Mn7LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|NUezNFcoRjJ|M{W3N|A4RC:jPh?=
N1E-115 MYjGeY5kfGmxbjDhd5NigQ>? NHrkdIoyKHWP NH7JPVY2KG2rboO= NXjNVnh7UW64ZYLz[UBi\2:waYP0JIFkfGm4aYT5JIF1KEOEMTDy[YNmeHSxcjDpckBud3W|ZTDONWUuOTF3IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhX0mQIEW1MFIyOi1{LXnu[JVk\WRiRWLLNU8zKHCqb4PwbI9zgWyjdHnvckBifCBzIIXNJJRz\WG2ZXSgOUBucW6|IIDybY9zKHSxIGfJUkA2PSx{MUKtNkBkcGGubHXu[4Uh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN3N{OwO{c,OjN|NUezNFc9N2F-
CHO NUXObGZ4TnWwY4Tpc44h[XO|YYm= NG\XSllCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHj1cYFvKEeSUkG4JJRz[W6|ZnXjeIVlKGmwIFPIU{Bk\WyuczDhd5Nme3OnZDDhd{Bl\Wy2YUmtWGhENWmwZIXj[YQh[mW2YT3hdpJme3SrbjDy[YNzfWm2bXXueEBqdmO3YnH0[YQhPjBibXnud{BxemmxcjD0c{Bl\Wy2YUmtWGhEKGGmZHn0bY9vKGK7IHLleIEu[XK{ZYP0bY4hfHKjboPsc4NifGmxbjDhd5NigSxiSVO1NF0yOC5zzszN Mlm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4N{m5OVUoRjJ|Nke5PVU2RC:jPh?=
HEK MnTwSpVv[3Srb36gZZN{[Xl? MXWxJIhz MULBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiCxdnXy[ZhxemW|c3XkJIlvKEiHSzDj[YxteyCjc4Pld5Nm\CCjczDbN|VUZUeWUHfhcY1iKGKrbnTpcoch[W[2ZYKgNUBpeiCkeTDsbZF2cWRic3PpcpRqdGyjdHnvckBkd3WwdHnu[{BidmGueYPpd{whTUN3ME2wMlAxOTYQvF2= MmLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{W1O|IoRjJ2MUe1OVczRC:jPh?=
CHOK1 M{G5WGZ2dmO2aX;uJIF{e2G7 NFHHbW4yKGi{ NFzsRWFFcXOybHHj[Y1mdnRib3[gX|NJZUOSNUW5OFAh\nKxbTDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKT1uxJINmdGy|IHHmeIVzKDFiaIKgZpkhdGmzdXnkJJNkcW62aXzsZZRqd25iY3;1cpRqdmdiYX7hcJl{cXNuIFvpQVAvODB3Od88US=> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5NUW3Nkc,OjRzN{W1O|I9N2F-
CHO NGfkUoFHfW6ldHnvckBie3OjeR?= M37YNFQ2KG2rboO= MXfBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiC|dHHicJkh\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDjc{1mgHC{ZYPzbY5oKGOxLXX4dJJme3OrbnegS4EyPS9zNjDhd5Nme3OnZDDhd{Bk[WylaYXtJIN2enKnboSgZYZ1\XJiNEWgcYlveyCkeTDmcJVwNTRiQV2gZZN{[XluIFnDOVA:OC5yMUROwG0> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR2NUOxNEc,OjR2NEWzNVA9N2F-
CHO MX\GeY5kfGmxbjDhd5NigQ>? NH;rPZo1PSCvaX7z MnzQRY51[WexbnnzeEBi[3Srdnn0fUBifCCFQkKgdoVk\XC2b4KgLJVvc26xd36gc5Jq\2mwKTDzeIFjdHliZYjwdoV{e2WmIHnuJGNJVyClZXzsd{Bkdy2neIDy[ZN{cW6pIHPvMYV5eHKnc4PpcochT2FzNT:xOkBie3Onc4Pl[EBieyClYXzjbZVuKGO3coLlcpQh[W[2ZYKgOFUhdWmwczDifUBndHWxLUSgRW0h[XO|YYmsJGlEPTB;OT64{txO M4e5Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NES1N|ExLz5{NES0OVMyODxxYU6=
CHO-K1 M2\aTGZ2dmO2aX;uJIF{e2G7 M1q3VFExKG2rboO= NV\o[WpsSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEOKTz3LNUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFPQMVU2QTRyLXnu[JVk\WRiW{O1V31IXFCpYX3tZXMh[mmwZHnu[{BqdmO3YnH0[YQh\m:{IEGwJI1qdnNicILpc5IhfG9iQ2CtOVU6PDBvY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDFiaIKgZpkh[mW2YTDjc5VvfGmwZzygT4k:OC5yMEG2{txO NUjLNpYzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA1QDRpPkK0PVAxPDh2PD;hQi=>
HEK293 Mn3FSpVv[3Srb36gZZN{[Xl? MkG1OlAhdWmwcx?= NVn6TIZJTGm|cHzhZ4Vu\W62IH;mJHs{UF2VUkG0NVcyPkFiZn;ycUBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bi\nSncjC2NEBucW6|IHL5JIJmfGFiY3;1cpRqdmduIFvpQVAvODBzON88US=> NE\oXFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFQ5PCd-MkS5NFA1QDR:L3G+
CHO-K1 M1XVUWZ2dmO2aX;uJIF{e2G7 MoK4OlAhdWmwcx?= MkGwSIl{eGyjY3Xt[Y51KG:oIGuzTH1EWC13NUm0NEBnem:vIHj1cYFvKEOEMjDy[YNmeHSxcjDlfJBz\XO|ZXSgbY4hS0iRLVuxJINmdGy|IHHmeIVzKDZyIH3pcpMh[nliYnX0ZUBkd3WwdHnu[{whU2l;MD61NlLPxE1? NEj2Xlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFQ5PCd-MkS5NFA1QDR:L3G+
CHO M3r1SGZ2dmO2aX;uJIF{e2G7 MUGyJIhzew>? MYDEbZNxdGGlZX3lcpQhd2ZiW{PIYWNRPTVuOUSwJIZzd21icnXjc41jcW6jboSgbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYX\0[ZIhOiCqcoOgZpkhdGmzdXnkJJNkcW62aXzsZZRqd25iY3;1cpRqdmduIFvpQVAvODF{Nt88US=> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEW2NUc,OjR7MEC1OlE9N2F-
CHO NVHQcGdSTnWwY4Tpc44h[XO|YYm= M13pRVIhcHK| NEnjUWtFcXOybHHj[Y1mdnRib3[gX|NJZUOSNUWsPVQxKG[{b32gdoVkd22kaX7hcpQhcHWvYX6gR2IzKHKnY3XweI9zKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZYZ1\XJiMjDodpMh[nlibHnxeYllKHOlaX70bYxt[XSrb36gZ492dnSrbnesJGtqRTBwOd88US=> M3fXbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwOVYyLz5{NEmwNFU3OTxxYU6=
CHO M2PmVWZ2dmO2aX;uJIF{e2G7 MoP1PVAhdWmwcx?= MmfRRYdwdmm|dDDhZ5Rqfmm2eTDheEBIWFJ3NTCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBEUE9iY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBNWElvaX7keYNm\CCkZYThMYFzemW|dHnuJJRz[W6|bH;jZZRqd25iYX\0[ZIhQTBibXnud{BjgSCudX3pcoV{[2WwY3WgZZN{[XluIFXDOVA:Oi5yMd88US=> NIfuVo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFU3OSd-MkS5NFA2PjF:L3G+
RD-HGA16 MWTGeY5kfGmxbjDhd5NigQ>? M2L0XWFvfGGpb37pd5Qh[WO2aY\peJkh[XRiQ1KxMW9ZOSCqZYTldo9lcW2ncjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBTTC2KR1GxOkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIH;y[ZhqdiCDLXnu[JVk\WRiY3HsZ4l2dSCvb3LpcIl7[XSrb36gZpkh\my3b4LvcYV1emmlIHntZYdqdmdicHzheIUhemWjZHXyJIFv[Wy7c3nzMEBM\T1yLkCwNe69VQ>? MknSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NES3N|QoRjJ2OUS0O|M1RC:jPh?=
RD-HGA16 M1LlcWZ2dmO2aX;uJIF{e2G7 NXTiVmlPSW62YXfvcol{fCCjY4Tpeol1gSCjdDDDRlEhemWlZYD0c5IhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iUlStTGdCOTZiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBEWDV3OUSwMYlv\HWlZXSgZ4Ft[2m3bTDtc4JqdGm8YYTpc44h[nliZnz1c5JwdWW2cnnjJIlu[WerbnegdIxifGVicnXh[IVzKGGwYXz5d4l{NCCNZU2wMlAxOTIQvF2= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl2NEezOEc,OjR7NES3N|Q9N2F-
Sf9 M3L6dWZ2dmO2aX;uJIF{e2G7 M4PzflIxKG2rboO= M1;CVmlvfmW{c3WgZYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIGPmPUBk\WyuczDjc4V5eHKnc4PpcochT2KndHGx[4FudWF{IHHu[EBTW0d2IHHzd4V{e2WmIHHzJIRm\3KjZHH0bY9vKG:oIGvnZY1u[S1|M2DdS3RRKGGodHXyJFIxKG2rboOgZpkhe3SnYXT5MZN1[XSnIFfUVIF{\SCjc4PhfUwhTUN3ME2wMlA2|ryP NHS5eG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC5OlI6Pyd-MkWwPVYzQTd:L3G+
Sf9 MlfySpVv[3Srb36gZZN{[Xl? M4LHeFIxKG2rboO= NULadIRlUW64ZYLz[UBi\2:waYP0JIFkfGm4aYT5JIF1KGi3bXHuJGNDOSC{ZXPldJRweiCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJINw\XiycnXzd4lv\yCJYnX0ZVFo[W2vYUKgZY5lKFKVR{SgZZN{\XO|ZXSgZZMh\GWpcnHkZZRqd25ib3[gX4didW2jLUOzVH1IXFBiYX\0[ZIhOjBibXnud{BjgSC|dHXh[Jkue3SjdHWgS3RR[XOnIHHzd4F6NCCHQ{WwQVAvODVyMUNOwG0> NHiwNJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC5OlI6Pyd-MkWwPVYzQTd:L3G+
CHO Ml;ZSpVv[3Srb36gZZN{[Xl? NFLrOFlCdnSjZ3;ubZN1KGGldHn2bZR6KGGpYXnud5QhS0JzUjCoeY5sdm:5bjDvdolocW5rIFPIU{Bk\WyuczDzeIFjdHliZYjwdoV{e2mwZzDHZYxxcGFzNjDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFPQOVU6PDBvaX7keYNm\CCrbnPy[YF{\SCrbjDpcpRz[WOnbHz1cIFzKGOjbHPpeY0hdGW4ZXygdJJmNXS{ZXH0[YQhOTBibXnud{Bj\W[xcnWgR3A2PTl2MDDzeIlufWyjdHnvckBjgSCvaXPyc5Bt[XSnIILlZYRmeiCkYYPl[EBie3OjeTygTWM2OD1yLkCxN|XPxE1? MonBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzNUGyN|EoRjJ4MUWxNlMyRC:jPh?=
CHO NGW3OopHfW6ldHnvckBie3OjeR?= M3m2bmFvfGGpb37pd5Qh[WO2aY\peJkh[WejaX7zeEBESjKUIDj1cotvd3ewIH;ybYdqdiliQ1jPJINmdGy|IIP0ZYJtgSCneIDy[ZN{cW6pIFfhcJBp[TF4IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiQ2C1OVk1OC2rbnT1Z4VlKGmwY4LlZZNmKGmwIHnueJJi[2WubIXsZZIh[2GuY3n1cUBt\X[nbDDwdoUufHKnYYTl[EAyOCCvaX7zJIJm\m:{ZTDDVFU2QTRyIIP0bY12dGG2aX;uJIJ6KG2rY4LvdIxifGVicnXh[IVzKGKjc3XkJIF{e2G7LDDJR|UxRTlwMd88US=> NGKyTGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG1NVI{OSd-Mk[xOVEzOzF:L3G+
HEK293 MX7GeY5kfGmxbjDhd5NigQ>? M3myWGludWWmaXH0[UBidnSjZ3;ubZN1N0mwdnXyd4Uh[WexbnnzeEBi[3Srdnn0fUBifCCqZX3h[4dtfXSrbnnuMZRi\2enZDDoeY1idiCFQkGgdoVk\XC2b4Kg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDhd5Nme3OnZDDhd{Bz\X[ncoPhcEBw\iBzIIXNJGNRPTVuOUSwMYlv\HWlZXSgbY5pcWKrdHnvckBw\iB3IIXNJIZwenOtb3zpck1qdmS3Y3XkJINCVVBiYXPjeY12dGG2aX;uJIJ6KGurbnX0bYMh[0GPUDDhd5NigQ>? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjJyM{[1PEc,OjZ{MEO2OVg9N2F-
HEK293 NH;xeGhHfW6ldHnvckBie3OjeR?= NIfiPVE6OCCvaX7z MlflSIl{eGyjY3Xt[Y51KG:oIGuzTH0uS1BvNUW5OFAh\nKxbTDoeY1idiC{ZXPvcYJqdmGwdDDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bi\nSncjC5NEBucW6|IHL5JGNwdXCndHn0bY9vKGKrbnTpcoch[XO|YYmsJGtq\XF;MD6wNVLPxE1? NHvre4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nke1OlA6Pyd-Mk[3OVYxQTd:L3G+
HEK293 Mn\oSpVv[3Srb36gZZN{[Xl? MWi5NEBucW6| MX;EbZNxdGGlZX3lcpQhd2ZiW{PIYU1EWC13NUm0NEBnem:vIHj1cYFvKHKnY3;tZolv[W62IFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iSFXLNlk{KGOnbHzzJIFnfGW{IEmwJI1qdnNiYomgR49ueGW2aYTpc44h[mmwZHnu[{Bie3OjeTygT4lmeT1yLke5{txO NVXEPFZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[3OVYxQTdpPkK2O|U3ODl5PD;hQi=>
CHO-K1 NV7vb4pUTnWwY4Tpc44h[XO|YYm= M{HpWmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiaIXtZY4hS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9vS{GgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCFUEW1PVQxNWmwZIXj[YQhcW62cnHj[YxtfWyjcjDjZYxkcXWvIH3vZoltcXqjdHnvckBjgSCFYXzj[YlvNTRiQV2td5RicW6rbnegZoF{\WRiRlzJVHIh[XO|YYmsJGtmRTBwMECxNe69VQ>? MnHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ6MkexN|coRjJ4OEK3NVM4RC:jPh?=
CHO NX3tcIg3TnWwY4Tpc44h[XO|YYm= MlXrPVAhe2Wlcx?= MXzJcpZmenOnIHHnc45qe3RiYXP0bZZqfHliYYSgbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCFSF:gZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcW62cnHj[YxtfWyjcjDjZYxkcXWvIH3vZoltcXqjdHnvckBi\nSncjC5NEB{\WO|IHL5JGNidGOnaX6tOEBCVS2|dHHpcolv\yCkYYPl[EBHVEmSUjDhd5NigSxiRVO1NF0xNjByNd88US=> NWXWZpdmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4NlcyOzdpPkK2PFI4OTN5PD;hQi=>
SJ-GBM2 M3PCeZFJXFNiYYPzZZk> MnHNdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= M371TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 NXzsNWZNeUiWUzDhd5NigQ>? NVLOUVkxeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFG2O|Mh[2WubIO= MmGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC MmrNdWhVWyCjc4PhfS=> NIjKcpRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=> M1zkb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 MY\xTHRUKGG|c3H5 NVXacldEeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz NYfJWVA5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-SH M1fyNpFJXFNiYYPzZZk> Mn\5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tV2gh[2WubIO= MmLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 NYX2eVdyeUiWUzDhd5NigQ>? NEPVfmhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz MojmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 NHLuTXhyUFSVIHHzd4F6 M{TKNJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCOQV6tOUBk\Wyucx?= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-12 MXHxTHRUKGG|c3H5 MVvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvMUKgZ4VtdHN? M{T3OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 M2TucJFJXFNiYYPzZZk> M1yxXJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCRSGOtOVAh[2WubIO= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
RD Ml7pdWhVWyCjc4PhfS=> MmfCdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
A673 NIfJ[ItyUFSVIHHzd4F6 NF65Sm1yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBCPjd|IHPlcIx{MQ>? MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC MVTxTHRUKGG|c3H5 NUjFbYtbeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= NGD3[5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
DAOY NG\GTIFyUFSVIHHzd4F6 NF;STVdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBFSU:\IHPlcIx{ NFnlXFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
TC32 MlexdWhVWyCjc4PhfS=> MoGzdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsdy=> NYnPUVk6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
U-2 OS MlnrdWhVWyCjc4PhfS=> NEDsT5dyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBWNTJiT2OgZ4VtdHN? NWHBR4dxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh41 NWjLcIlGeUiWUzDhd5NigQ>? NYDWT3RWeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? M13WdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 M3r2[5FJXFNiYYPzZZk> NXf6[WF4eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy| NX[5XZNwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
CHOK1 M3fYb2Z2dmO2aX;uJIF{e2G7 MkjoPVAhdWmwcx?= MXHJcpZmenOnIHHnc45qe3RiYXP0bZZqfHliYYSgR{11\XKvaX7hcIx6KHC{b3zpcosufGGpZ3XkJI1wfXOnIFPCNkBz\WOncITvdkBmgHC{ZYPz[YQhcW5iQ1jPT|Eh[2WubIOgbIFz[m:{aX7nJIJmfGFvZ3HsZYN1d3OrZHHz[UBmdnq7bXWg[pV{\WRiYnX0ZU1ienKnc4TpckBie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDi[ZRiNWG{cnXzeIlvKHKnY4L1bZRu\W62IHHmeIVzKDlyIH3pcpMh[nliY3jlcYltfW2rbnXzZ4VvfCCjc4PhfUwhTUN3ME2wMlAyPc7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTl|OUe0OEc,Ojl7M{m3OFQ9N2F-
HEK293 NVTyUYZETnWwY4Tpc44h[XO|YYm= MnvORY51[WexbnnzeEBi[3Srdnn0fUBifCCqdX3hckBESjFicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCkeTDHWHBo[W2vYWOgZolv\GmwZzDhd5NigSxiS3W9NE4xODB{zszN NUTrfVFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwO|c3ODlpPkOwNFc4PjB7PD;hQi=>
HEK293 NYn6PWpKTnWwY4Tpc44h[XO|YYm= M{fDUVkxKHOnY4O= M4rqfmlvfmW{c3WgZYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDDRlEhemWlZYD0c5Ih\XiycnXzd4VlKGmwIFjFT|I6OyClZXzsd{Bkdy2neIDy[ZN{cW6pIFfhcJBp[XFzNjDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPhcINqfW1ibX;ibYxqgmG2aX;uJIFnfGW{IEmwJJNm[3NiYomgZ4Ft[2Wrbj20JGFCVSCmeXWtZoF{\WRiRlzJVHIh[XO|YYmsJGVEPTB;MD61PFbPxE1? NH3RWlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC3O|YxQSd-M{CwO|c3ODl:L3G+
HEK293 NUW4cHFDTnWwY4Tpc44h[XO|YYm= MnPjN|AhdWmwcx?= MVrJcpZmenOnIHHnc45qe3RiYXP0bZZqfHliYYSgbJVu[W5iQ1KxJJJm[2WydH;yJIV5eHKnc4Pl[EBqdiCKRVuyPVMh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDmc5J{c2:uaX6tbY5lfWOnZDDjRW1RKGGlY4XteYxifGmxbjDh[pRmeiB|MDDtbY5{KGK7IFjUVmYh[XO|YYmsJGVEPTB;MD6wNFQy|ryP MkDaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB|M{mzPFcoRjNyM{O5N|g4RC:jPh?=
CHO NGXmVndHfW6ldHnvckBie3OjeR?= NGq3Z5NFcXOybHHj[Y1mdnRib3[gX|NJZS2FUEW1PVQxKG[{b32gbJVu[W5icnXjc41jcW6jboSgZ4FvdmGkaX7vbYQhS0JzIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[nlicnHkbY9tcWejbnSgZolv\GmwZzDhd5NigSxiS3m9NE4yPDQQvF2= M4jEVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNkC5OlA5Lz5|MU[wPVYxQDxxYU6=
CHO M3ry[mZ2dmO2aX;uJIF{e2G7 MnPMRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHVHI2PSCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDMVGkucW6mdXPl[EBj\XSjLXHydoV{fGmwIILlZ5J2cXSvZX70JIJ6KGKndHGt[4Ft[WO2b4Pp[IF{\SCnbor5cYUh\nKjZ33lcpQh[2:vcHzlcYVvfGG2aX;uJI1mfGixZDygSWM2OD1{LkCx{txO NHW4VWM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTFxJ{7DbGVOSkx:L3G+
CHO MYPGeY5kfGmxbjDhd5NigQ>? MmewRYdwdmm|dDDhZ5Rqfmm2eTDheEBpfW2jbjDHVHIyQCCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDi[ZRiNWG{cnXzeIlvKHKnY4L1bZRu\W62IHL5JIJmfGFvZ3HsZYN1d3OrZHHz[UBmdnq7bXWg[pJi\22nboSgZ49ueGynbXXueIF1cW:wIH3leIhw\CxiRVO1NF0yOC5zzszN M4O5W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyOS9pPlPoSW1DVDxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-LRP6 / LRP6 / Lgr5 ; 

PubMed: 29354056     


Representative Western blot analysis of Lgr5, LRP6 and phosphorylated (Ser1490) LRP6. GAPDH was used as loading control.

β-catenin; 

PubMed: 29354056     


Representative Western blot analysis (left) of β-Catenin in total protein lysates from GTG7 cells. Right, densitometric analysis of β-Catenin normalized vs. GAPDH, used as loading control. Results are expressed as means ± SD of 3 independent experiments performed at least in duplicate (∗∗p < 0.01).

CD44 / Lgr5 / CD133 / EpCAM ; 

PubMed: 29354056     


Representative Western blot analysis of CD44, Lgr5, CD133 and EpCAM in total protein lysates from HCT116 cells. GAPDH was used as loading control.

29354056
Immunofluorescence
Lgr5 ; 

PubMed: 29354056     


Confocal microscopy analysis of Lgr5 (red fluorescence) in GTG7 cells treated with Rimonabant at indicated doses for 18 h. Nuclei were stained with Hoechst (blue fluorescence); GFP (green); Scale bar = 5 μm.

β-catenin; 

PubMed: 29354056     


Confocal microscopy analysis of β-Catenin (red) in GTG7 cells treated with Rimonabant at indicated doses for 18 h. Nuclei were stained with Hoechst (blue fluorescence); GFP (green); Scale bar = 5 μm.

29354056
体内研究

Rimonabant腹腔内给药或口服给药有效且剂量依赖性拮抗大麻素受体激动剂的经典药理学和行为作用。[3]在氧化偶氮甲烷诱发的结肠癌模型中,结肠直肠癌发生之前,Rimonabant显著减少异常隐窝病灶(ACF)的形成。[4] Rimonabant (10 mg/kg,强饲)对3个月大雄性肥胖Zucker大鼠喂食2周作为葡萄糖耐量降低模型,对6个月大雄性肥胖Zucker大鼠喂食10周作为代谢综合征模型。相对于瘦的Zucker大鼠,RANTES (正常T细胞表达和分泌的活性调节蛋白)和MCP-1 (单核细胞趋化蛋白-1)血清水平在肥胖Zucker大鼠体内增加,而在Rimonabant长期治疗下显著降低,这表明代谢综合征大鼠的体重增加。相对于瘦的Zucker大鼠,Neutrophils和单核细胞在年老和幼小的肥胖Zucker大鼠中显著增加,但是被Rimonabant降低。相对于瘦的Zucker大鼠,血小板结合的纤维蛋白原在两个年龄段肥胖Zucker大鼠中显著增加,但是被Rimonabant减少。来自肥胖大鼠的血小板对凝血酶诱导的聚集更敏感,并粘附在纤维蛋白原上,这都会被Rimonabant治疗减弱。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

配体结合试验:

将人CB1和CB2稳定转染的HEK 293细胞和细胞膜纯化。0.2-8 μg纯化的细胞膜与0.75 nM [3H] CP55,940和Rimonabant在孵育液(50 mM Tris-HCl,5 mM MgCl2,1 mM EDTA,0.3%BSA,pH 7.4)中培育。非特异性结合在1 μM CP55,940存在下定义。反应在30 °C下,Multiscreen中培育1.5小时。反应通过多重过滤终止,并且用冰预冷的洗涤缓冲液(50mM Tris,pH 7.4,0.25% BSA)清洗4次。过滤器上的放射性结合通过Topcount测量。IC50定义为抑制50%[3H] CP55,940结合时Rimonabant的浓度,并通过非线性回归计算。
细胞实验:

[2]

- 合并
  • Cell lines: Raw 264.7细胞
  • Concentrations: 0,1,4 μM
  • Incubation Time: 17小时
  • Method:

    Raw 264.7细胞(2×106/孔)在12孔板中用PBS漂洗,培养基用不同量Rimonabant增补,1小时后用7-酮基胆固醇(7KC)增补。所有孔加入等量的载体。培育16小时后,caspase-3和caspase 3样活性使用荧光底物(Ac-DEVD-AFC)和配备酶标仪的分光光度计测定。


    (Only for Reference)
动物实验:

[3]

- 合并
  • Animal Models: 雄性小鼠和雄性大鼠
  • Dosages: 20 ml/kg (小鼠) 和 5 ml/kg (大鼠)
  • Administration: WIN55212-2静脉注射之前,Rimonabant 通过腹腔注射(30 分钟)或口服(1 小时)给药
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 25 mg/mL (53.9 mM)
Water Insoluble
Ethanol '''''2 mg/mL
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+dd H2O
5 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 463.79
化学式

C22H21Cl3N4O

CAS号 168273-06-1
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00750347 Completed Drug: Rimonabant and Paracetamol and placebo Pain University Hospital Clermont-Ferrand September 2008 Phase 1
NCT00656487 Completed Drug: rimonabant|Drug: placebo Cannabis Dependence|Cannabis Withdrawal The Scripps Research Institute|National Institute on Drug Abuse (NIDA) April 30 2008 Phase 2
NCT00546325 Completed Drug: Rimonabant|Drug: Placebo Diabetes Mellitus Type 2 Sanofi October 2007 Phase 3
NCT00525681 Completed Drug: cyclosporine A|Drug: tacrolimus Renal Transplantation University of Oslo School of Pharmacy September 2007 Phase 4
NCT00584389 Terminated Drug: rimonabant|Behavioral: Dietary intervention Obesity University of Surrey|European Foundation for the Study of Diabetes|Royal Surrey County Hospital NHS Foundation Trust July 2007 Phase 4
NCT01041170 Completed Drug: THC|Drug: Rimonobant|Drug: Rimonabant Cannabis|Dependence National Institute on Drug Abuse (NIDA)|National Institutes of Health Clinical Center (CC) April 16 2006 Phase 1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID