Rotenone (Barbasco)

For research use only. Not for use in humans.

目录号:S2348 别名: Barbasco, Dactinol, Paraderil, Rotenon, Rotocide

Rotenone (Barbasco) Chemical Structure

CAS No. 83-79-4

Rotenone (Barbasco, Dactinol, Paraderil, Rotenon, Rotocide) 是一种植物杀虫剂,是线粒体电子传递mitochondrial electron transport的抑制剂。Rotenone 可抑制 NADH/DB oxidoreductaseNADH oxidase,对应的IC50值分别为28.8 nM和5.1 nM。Rotenone 可通过增强线粒体活性氧的产生来诱导凋亡。

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产品安全说明书

NADPH-oxidase抑制剂选择性比较

生物活性

产品描述 Rotenone (Barbasco, Dactinol, Paraderil, Rotenon, Rotocide) 是一种植物杀虫剂,是线粒体电子传递mitochondrial electron transport的抑制剂。Rotenone 可抑制 NADH/DB oxidoreductaseNADH oxidase,对应的IC50值分别为28.8 nM和5.1 nM。Rotenone 可通过增强线粒体活性氧的产生来诱导凋亡。
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MG63 MonuSpVv[3Srb36gZZN{[Xl? M3K1[FEh|ryP MnnQNUBpeg>? NWTmOVBZUW6lcnXhd4UhcW5ibHHjeIF1\SCycn;keYN1cW:wIHnuJG1IPjNiY3XscJMh[XRiMTD1UUBi\nSncjCxJIhzKGK7IFXMTXNC NUK5cGVFOTdyOEi0PFk>
HepG2 NXvSRVZXS2WubDD2bYFjcWyrdImgZZN{[Xl? Mk\aNlQhcA>? NVLRRph2SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xOUDPxE1? MXuxO|Q5Ojh{NB?=
A549 MnXBR4VtdCC4aXHibYxqfHliYYPzZZk> NIfzVmYzPCCq NVLIW5ZrSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjCyOEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUK2JO69VQ>? NUniOlBkOTd2OEK4NlQ>
U937 M{i3[nBzd2yrZnXyZZRqd25iYYPzZZk> NHzMTWk4OiCq MlvCRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCXOUO3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCZU2StPEBie3OjeR?= MWixO|E2QDB3NB?=
U937 Mn\uVJJwdGmoZYLheIlwdiCjc4PhfS=> MVO0PEBp M{fSb2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVUmzO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliV2PUMVgh[XO|YYmsJGlEPTB;MD6yJO69VQ>? MY[xO|E2QDB3NB?=
U937 NVr5fXFzWHKxbHnm[ZJifGmxbjDhd5NigQ>? MVOyOEBp MVvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV7M{egZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFeVVD24JIF{e2G7LDDJR|UxRTF|IN88US=> NYewOoN6OTdzNUiwOVQ>
T47D MXrGeY5kfGmxbjDhd5NigQ>? MUmwMlAxOSC2bzCwMlEh|ryP NHzJcpFKdmirYnn0bY9vKG:oIHPlcIx2dGG{IILld5BqemG2aX;uJIlvKGi3bXHuJHQ1P0RiY3XscJMh[XO|ZYPz[YQh[XNicnH0[UBw\iCxeInn[Y4h[2:wc4XtdJRqd25iYYSgNE4xODFidH:gNE4yKHWP NGfGPJQzOTh5NUGxOC=>
T47D MlrMSpVv[3Srb36gZZN{[Xl? M2DOS|Q5KGh? M{i2NHJm\HWldHnvckBqdiCDVGCgcIV3\WxiaX6gbJVu[W5iVES3SEBk\WyuczDh[pRmeiB2ODDodpMh[nliSGDMR{1WXiCjbnHsfZNqew>? MUWyNVg4PTFzNB?=
MDA-MB-231 NXO5W3BsTnWwY4Tpc44h[XO|YYm= Mn\nNE4yNTFizszN M{fmUlMxKG2rbh?= MUHJcohq[mm2aX;uJI9nKG2WT2KgbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDhd5Nme3OnZDDhd{BmUUZ{YXzwbIEhcHmyZYLwbI9{eGixconsZZRqd25ib36gV4VzKDVzIHH0JFAvOSC2bzCxJJVOKGGodHXyJFMxKG2rboOgZpkhX2W|dHXyckBjdG:2dHnu[y=> MoHsNlE5PzVzMUS=
Hepa-1c1c7 NIP4NmtEgXSxdH;4bYNqfHliYYPzZZk> NHK5OFBEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBJ\XCjLUHjNYM4KGOnbHzzMEBKSzVyPUCuO{DPxE1? MV6yN|g6PTBzOR?=
697 cells M4jIUGN6fG:2b4jpZ4l1gSCjc4PhfS=> MkfPO|IhcA>? NYjxW2EyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hPjl5IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEmFNUC9NE4{KM7:TR?= NHv6blgzOzh7NUCxPS=>
697 cells NF;aUIxEgXSxdH;4bYNqfHliYYPzZZk> M{Hwc|Q5KGh? NULleZNRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hPjl5IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFNiYYPzZZktKEmFNUC9NE4{KM7:TR?= NGPuTXczOzh7NUCxPS=>
Raji MoH6R5l1d3SxeHnjbZR6KGG|c3H5 M1fOUVczKGh? MVvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDSZYpqKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGlEPTB;MT6xJO69VQ>? NVPtOnA6OjN6OUWwNVk>
Raji NV3YTVRwS3m2b4TvfIlkcXS7IHHzd4F6 MX:3NkBp NWXmN5ljS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWmGsaTDj[YxteyCjZoTldkA1QCCqcoOgZpkhVVSVIHHzd4F6NCCLQ{WwQVQvOiEQnH2= MYeyN|g6PTBzOR?=
HT-29 MYDDfZRwfG:6aXPpeJkh[XO|YYm= M4LxOFczKGh? NYHHeW86S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUFRvMkmgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF0xNjNizszN NHzjPHkzOzh7NUCxPS=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
sestrin2 ; 

PubMed: 26031332     


Mes 23.5 cells were treated with DMSO or rotenone at the indicated concentrations for 12 h. After rotenone treatment, cells were harvested and immunoblotted for sestrin2 protein levels.

p-AMPK / AMPK ; 

PubMed: 26031332     


Cells were treated with rotenone and immunoblotted for phosphorylated AMPK (Thr172) and total AMPK. 

p-mTOR / mTOR / p-S6K1 / S6K1 / p-4EBP1 / 4EBP1 ; 

PubMed: 25304210     


Neuronal cells were treated with (A) rotenone (0–1 μM) for 24 h followed by Western blotting using the indicated antibodies. The blots were probed for β-tubulin as a loading control. 

p-CaMKII / CaMKII ; 

PubMed: 26859572     


Rotenone resulted in phospho-CaMKII increase in a concentration-dependent manner in the cells.

26031332 25304210 26859572
Immunofluorescence
p65 ; 

PubMed: 23977201     


BV2 cells were treated with rotenone for 6 hours or LPS for 1 hour. The cells were fixed and labeled with DAPI (blue) or anti-p65 antibodies (red). The scale bar represents 10 µm.

activated caspase-1 ; 

PubMed: 23977201     


BV2 cells were treated with 1 µM rotenone or 1 µg/mL LPS for 24 hours and then stained with green fluorescent probes for activated caspase-1 for 1 hour. The scale bar represents 10 µm.

23977201
Growth inhibition assay
Cell viability; 

PubMed: 27045591     


PC12 cells were treated with 0~100 μmol/L (μM) rotenone for 24 hours. Cell viability was measured as percentage relative to vehicle (*P < 0.05; **P < 0.01).

27045591

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

体外 DMSO 55 mg/mL (139.44 mM)
Ethanol 2 mg/mL (5.07 mM)
Water Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 394.42
化学式

C23H22O6

CAS号 83-79-4
储存条件 粉状
溶于溶剂
别名 Barbasco, Dactinol, Paraderil, Rotenon, Rotocide

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID