Vinblastine (NSC-49842) sulfate

For research use only. Not for use in humans.

目录号:S4505 别名: Vincaleukoblastine sulfate salt, 29060-LE, Exal, Velban, Velbe 中文名称:硫酸长春碱

Vinblastine (NSC-49842) sulfate Chemical Structure

CAS No. 143-67-9

Vinblastine sulfate (NSC49842, Vincaleukoblastine sulfate salt, 29060-LE, Exal, Velban, Velbe)抑制微管形成nAChR活性,在无细胞实验的测定中,IC50为8.9 μM。Vinblastine sulfate 可诱导自噬和凋亡。

规格 价格 库存 购买数量  
RMB 573.88 现货
RMB 2023.45 现货
RMB 5545.08 现货
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客户使用Selleck生产的Vinblastine (NSC-49842) sulfate发表文献25篇:

产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Vinblastine sulfate (NSC49842, Vincaleukoblastine sulfate salt, 29060-LE, Exal, Velban, Velbe)抑制微管形成nAChR活性,在无细胞实验的测定中,IC50为8.9 μM。Vinblastine sulfate 可诱导自噬和凋亡。
靶点
nAChR [1]
(Adrenal Chromaffin Cells)
8.9 μM
体外研究

Vinblastine的平均终末半衰期为14.3小时。Vinblastine加入到新鲜分离的大鼠肝细胞,可迅速穿透到细胞当中[3]。Vinblastine抑制由肾上腺髓质素诱导的血管生成反应,还可引起M期阻滞[4]。在特定浓度下,vinblastine能显著增加微核单核细胞的数量[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
COLO 320 human colorectal carcinoma cell line M1rsdWZ2dmO2aX;uJIF{e2G7 NGDUZlNKdiC4aYTyc{Bkd26lZX70doF1cW:wIILldZVqemWmIITvJItqdGxiNUClJI9nKEORTF:gN|IxKGi3bXHuJINwdG:{ZXP0ZYwh[2G{Y3nuc41iKGOnbHygcIlv\SxiRVO1NF0xNjB6IN88US=> NVfLcmZyOTJ|NkGzPVc>
LNCaP human prostate cancer cell line NFPUXZZHfW6ldHnvckBie3OjeR?= M3PBV2lvKH[rdILvJINwdmOnboTyZZRqd25icnXxeYlz\WRidH:gb4ltdCB3MDWgc4YhVE6FYWCgbJVu[W5icILvd5RifGViY3HuZ4VzKGOnbHygcIlv\SxiRVO1NF0xNjVizszN MnvNNVI{PjF|OUe=
K562 cell M4DqOmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHzQNHFKdiC4aYTyc{BqdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDoeY1idiClaILvcolkKG27ZXzv[4Vvd3W|IHzleYtmdWmjIFu1OlIh[2WubDDndo94fGhuIFnDOVA:OC5yMEGg{txO NYHrXFVPOTJ6NUK3Olg>
T47D cells MkKxSpVv[3Srb36gZZN{[Xl? MWHJckB3cXS{bzDjc45k\W62cnH0bY9vKHKncYXpdoVlKHSxIHvpcIwhPTBnIH;mJHQ1P0RiaIXtZY4h[nKnYYP0JIR2[3SjbDDjZZJkcW6xbXGgZ4VtdCCuaX7lMEBGSzVyPUCuNFgh|ryP NET5flAyOjN4MUO5Oy=>
K562 cell MUfQdo9tcW[ncnH0bY9vKGG|c3H5 M{\mfFQ5KGh? NGfIeVBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFu1OlIh[2WubIOgZYZ1\XJiNEigbJJ{NCCLQ{WwQVAvODBzIN88US=> MUmxPVIzODBzOB?=
ACHN cells NYfIR5YyS3m2b4TvfIlkcXS7IHHzd4F6 M1v6RlQ5KGh? Mnj3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRWNJViClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUKyMlch|ryP MV2xPVQ3Pzh5Nx?=
A375 cells MWHDfZRwfG:6aXPpeJkh[XO|YYm= NUjrO41GPDhiaB?= NWX6PJhzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTN5NTDj[YxteyCjZoTldkA1QCCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvOiEQvF2= NW\pRZMyOTl2Nke4O|c>
C32 cells MkTsR5l1d3SxeHnjbZR6KGG|c3H5 NVH0[JB{PDhiaB?= M1LCTGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGM{OiClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUOg{txO M{Lnc|E6PDZ5OEe3
LNCAP cells Ml7ER5l1d3SxeHnjbZR6KGG|c3H5 NUn2R3d2PDhiaB?= M4\SbmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxPS0GSIHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUC9NlkvOyEQvF2= NILqfGcyQTR4N{i3Oy=>
Huh-7D12 cells NVvHSFFFS3m2b4TvfIlkcXS7IHHzd4F6 Ml3kOFghcA>? MkH2R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpNTeGMUKgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF01PS54IN88US=> NIDIVpgyQTR4N{i3Oy=>
COR-L23 cells NW[wfW86S3m2b4TvfIlkcXS7IHHzd4F6 NX[zb2p1PDhiaB?= MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDU3IuVDJ|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUC9OFUvPSEQvF2= NXzwdI4xOTl2Nke4O|c>
142BR cells NELTW2xEgXSxdH;4bYNqfHliYYPzZZk> NFXJRoM1QCCq MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjCxOFJDWiClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUO3MlYh|ryP NGGy[lcyQTR4N{i3Oy=>
HT-29 cells M{mzTmN6fG:2b4jpZ4l1gSCjc4PhfS=> MknQOFghcA>? M2f4UmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhVNTJ7IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFNiYYPzZZktKEmFNUC9NE42PSEQvF2= M{nOe|IyQTJyN{[y
A549 cells MmTLVJJwdGmoZYLheIlwdiCjc4PhfS=> NGSyZVY1QCCq MmHDRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:Oi5|NjFOwG0> NVi3fWV4OjJ3NE[2O|Q>
DU145 cells NXvke4R3WHKxbHnm[ZJifGmxbjDhd5NigQ>? MorROFghcA>? NFj4WXdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTVNVQ2KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO|YYmsJGlEPTB;ND6yOUDPxE1? NV\6OFNVOjJ3NE[2O|Q>
SK-MEL-5 cells NYLNbI44WHKxbHnm[ZJifGmxbjDhd5NigQ>? M4H3V|Q5KGh? NI\CWnZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLMW1GVC13IHPlcIx{KGGodHXyJFQ5KGi{czDifUBOXFNiYYPzZZktKEmFNUC9NU44PCEQvF2= M{fnWVIzPTR4Nke0
HepG2 cells NUe0SJRFWHKxbHnm[ZJifGmxbjDhd5NigQ>? NGD2Nog1QCCq NFnEOJRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjldGczKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHMh[XO|YYmsJGlEPTB;MD6xOkDPxE1? NYLvSZdXOjJ3NE[2O|Q>
HT-29 cells NHzNdmNRem:uaX\ldoF1cW:wIHHzd4F6 MUG0PEBp MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWLUK5JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:OTFwMUig{txO NV35cW11OjJ3NE[2O|Q>
MCF7 cells MlPzVJJwdGmoZYLheIlwdiCjc4PhfS=> NI[1TGo1QCCq MnLxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:OjRwMEig{txO NIf3XXUzOjV2Nk[3OC=>
MDA-MB-231 cells NYWyNIF[WHKxbHnm[ZJifGmxbjDhd5NigQ>? M4fUOlQ5KGh? NUHjeYJ6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:OzFwNUKg{txO M2nLcVIzPTR4Nke0
rat REF52 cells M4T4[GZ2dmO2aX;uJIF{e2G7 NEC2[lMxNjFizszN Ml2zN|AhdWmwcx?= Mn;xTY5lfWO2aX;uJI9nKG2rY4LveJVjfWynIHTldI9tgW2ncnn6ZZRqd25iaX6gdoF1KFKHRkWyJINmdGy|IHH0JFAvOSC3TTDh[pRmeiB|MDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeR?= M{T5WVI{QTR5OEK2
HepG2 cells MXfDfZRwfG:6aXPpeJkh[XO|YYm= MVe3NkBp MlfsR5l1d3SxeHnjbZR6KGGpYXnud5Qh[WS{aXHtfYNqdi2{ZYPpd5RidnRiaIXtZY4hUGWyR{KgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkC1OkDPxE1? MXiyN|cxQDBzMB?=
HepG2 cells MomwR5l1d3SxeHnjbZR6KGG|c3H5 NHu5[Yw4OiCq MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvODF7IN88US=> NHL3RmkzOzdyOECxNC=>
K562 cells NHvuSo9EgXSxdH;4bYNqfHliYYPzZZk> NHrxN3Y4OiCq MmXnR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvODF4IN88US=> NXTnWJJ7OjN5MEiwNVA>
MDA-MB-231 cells NH:zO2NEgXSxdH;4bYNqfHliYYPzZZk> NX;aWo1lPzJiaB?= NIruS|FEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE4xODh|IN88US=> NYXF[XRXOjN5MEiwNVA>
MCF7 cells NFfkT|REgXSxdH;4bYNqfHliYYPzZZk> NHu1ZlM4OiCq NF\LOlNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC5yMEeg{txO NF\X[FIzOzdyOECxNC=>
SCL6 cells M1\LbWN6fG:2b4jpZ4l1gSCjc4PhfS=> NHnyc4lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUS0x4IHPlcIx{KGK7IF3UWEBie3OjeTygSWQ2OD14LkGg{rxu NIS0RWwyOjh6MEOxOC=>
SCL9 NUDxSnJmS3m2b4TvfIlkcXS7IHHzd4F6 NFjPfFJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUS0x7IHPlcIx{KGK7IF3UWEBie3OjeTygSWQ:PS5|IN88US=> NF\zZosyOjh6MEOxOC=>
KATO III cells MmrqR5l1d3SxeHnjbZR6KGG|c3H5 NX\DUZlsS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0GWTzDJTWkh[2WubIOgZpkhVVSWIHHzd4F6NCCHREWwQVYvOSEQvF2= MWmxNlg5ODNzNB?=
NUGC4 cells NI\kXlVEgXSxdH;4bYNqfHliYYPzZZk> MULDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDOWWdEPCClZXzsd{BjgSCPVGSgZZN{[XluIFXEOVA:PS5|IN88US=> NYK4bm51OTJ6OECzNVQ>
UACC903 cells NGHlVohEgXSxdH;4bYNqfHliYYPzZZk> M4DqOlQ5KGh? M3jYe2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHVCS0N7MEOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WUzDhd5NigSxiSVO1NF0yNjZ3IN88US=> Mn\hNlE6OjB5NkK=
K562 cells NVf4[mJ1TnWwY4Tpc44h[XO|YYm= NXrjclk3UW6qaXLpeIlwdiCxZjD0eYJ2dGmwIIDvcJlu\XKrenH0bY9vKGmwIHj1cYFvKEt3NkKgZ4VtdHNuIFnDOVA:OC5zMzFOwG0> MYSyNFU1Pjl6MB?=
BxPC3 cells MUjQdo9tcW[ncnH0bY9vKGG|c3H5 NInBbWs1QCCq MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEK6UFOzJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGOgZZN{[XluIFnDOVA:OS5zMzFOwG0> M1HIOVIzPTR4Nke0

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
GRP78 ; 

PubMed: 19674193     


Taxol and vinblastine induce GRP78 expression in breast cancer cells. (A) ZR75-30 cells were treated with taxol and vinblastine for 24 hrs. Cell lysates were harvested and subjected to Western blot analysis with anti-GRP78 antibody. -actin was probed as a loading control. (B) Western blot analysis of GRP78 expression in response to taxol and vinblastine treatments in T47D cells. (C) Western blot analysis of GRP78 expression in response to taxol and vinblastine treatments in MCF-7 cells. (D) MCF-7 cells were treated with 0.5 μM taxol and 0.1 μM vinblastine for indicated hours. Cell lysates were harvested and subjected to Western blot analysis with anti-GRP78 antibody.

p-eIF2; 

PubMed: 19674193     


MCF-7 cells were treated with 0.5 μM taxol and 0.1 μM vinblastine for indicated periods. Total proteins were harvested and subjected to Western blot analysis of the phosphorylated eIF2α.

p-JNK / c-Caspase-7 / c-PARP ; 

PubMed: 19674193     


MCF-7 cells were treated with indicated doses of taxol and vinblastine for 24 hrs. Total proteins were harvested and subjected to Western blot analysis of the phosphorylated JNK, cleaved caspase-7 and PARP.

ERK / p-ERK / Mcl-1 / Bad / Bid / Noxa ; 

PubMed: 20371726     


Expression of Bcl-2 family members and activation of MAPK proteins in the panel of cell lines. The indicated cell lines were either undamaged or incubated with 2.2 μM vinblastine for 4 h. Lysates were analyzed by immunoblotting for expression of the indicated proteins.

19674193 20371726
Immunofluorescence
α-tubulin / Acetyl tubulin ; 

PubMed: 30120268     


Differentiated SH-SY5Y cells were incubated in the absence and presence of indibulin (25 nM), vinblastine (10 nM) and colchicine (10 nM) for 24 h. Cells were fixed and processed for immunostaining with antibodies against α-tubulin (green) and acetylated tubulin (red). Cells were observed at two magnifications under a microscope. Scale bar = 100 and 10 μm in low (Left panel) and high (Right panel) magnification, respectively.

30120268
Growth inhibition assay
Cell viability; 

PubMed: 27114800     


Panel (A) shows cell viability (%) of the cells after 24 and 48 hrs incubation with vinblastine (0-100 nM) or docetaxel (0-100 nM). 

27114800
体内研究 Vinblastine作为抗癌剂被广泛运用,但具有一些意外的副作用[6]。Vinblastine和RAP在低浓度下的结合使用,可在体内得到比较满意的抗血管形成作用[4]。在临床使用剂量下,vinblastine抑制CEM细胞中的微管蛋白的棕榈酰化[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[2]

- 合并
  • Cell lines: 中国仓鼠卵巢细胞(CHO)
  • Concentrations: 1% (v/v) (dissolved in DMSO)
  • Incubation Time: 3 h
  • Method:

    6孔细胞培养板,每孔细胞密度为5×104 cells/mL,悬浮于3 mL培养基中。向其中加入vinblastine孵育3小时,生长21小时后进行后续检测。


    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL (110.0 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 909.05
化学式

C46H58N4O9.H2SO4

CAS号 143-67-9
储存条件 粉状
溶于溶剂
别名 Vincaleukoblastine sulfate salt, 29060-LE, Exal, Velban, Velbe

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02840409 Recruiting Drug: Vinblastine|Drug: Bevacizumab Low Grade Glioma The Hospital for Sick Children|Hoffmann-La Roche August 2016 Phase 2
NCT02670707 Recruiting Drug: Cytarabine|Drug: Vinblastine/prednisone Langerhans Cell Histiocytosis Baylor College of Medicine March 7 2016 Phase 3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID