Apigenin (LY 080400)

For research use only. Not for use in humans.

目录号:S2262 别名: NSC 83244 中文名称:芹菜素,芹黄素,芹菜苷元

Apigenin (LY 080400) Chemical Structure

CAS No. 520-36-5

Apigenin (NSC 83244, LY 080400)是一种有效的CYP2C9抑制剂,Ki为2 μM.。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 735.38 现货
RMB 580.57 现货
RMB 990.11 现货
RMB 1714.91 现货
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客户使用Selleck生产的Apigenin (LY 080400)发表文献17篇:

客户使用该产品的4个实验数据:

  • Western blotting confirmed that both GLUT-1 (A) and p-Akt (B) were expressed in Hep-2 cells in different apigenin and cisplatin concentration.

    Int J Clin Exp Pathol, 2014, 7(7):3938-3.. Apigenin (LY 080400) purchased from Selleck.

  • SKH-1 mice were subjected to UVB radiation (1000 J/m2 daily, 5 days), or topical apigenin (Api, 5 μmol, in 200 μl vehicle, DMSO: acetone 1:9) was applied 1 h prior to each UVB exposure. Mice were also sham-irradiated and treated with apigenin, and the control group of mice was subjected to sham irradiation and vehicle. 24 h after the final UVB exposure, the mice were euthanized, dorsal skin was harvested, fixed in formalin and paraffin-embedded. (A) Representative Hematoxylin and Eosin (H&E) staining of skin sections (scale bar, 100 μm). (B) Quantitation of epidermal thickness (mean ± SD), *, P < 0.001; **, P< 0.0001. Three sections per mouse and 3 mice per group were evaluated.

    Cell Signal, 2016, 28(5):460-468. Apigenin (LY 080400) purchased from Selleck.

  • The effects of apigenin and GLUT-1 AS-ODNs on xenograft apoptosis (the second experiment), observed an under optical microscope (magnification, ×400), and the percentage of apoptotic cells in 100 cells per field was counted and used to calculate the mean apoptosis index (AI). AI, apoptotic index.

    Oncol Rep, 2015, 34(4):1805-14. Apigenin (LY 080400) purchased from Selleck.

  • GLUT-1 mRNA expression levels were significantly reduced in the ACC-2 human adenoid cystic carcinoma cells following treatment with increasing doses of apigenin (P<0.05), as determined by reverse transcription-quantitative polymerase chain reaction. Following treatment with 10 µM apigenin, the expression levels of GLUT-1 mRNA did not vary significantly with increasing treatment duration (P>0.05). Following treatment with 40 and 160 µM apigenin, the expression levels of GLUT-1 mRNA were significantly reduced with increasing treatment duration. *P<0.05 vs. control. GLUT-1, glucose transporter-1.

    Mol Med Rep, 2015, 12(5):6461-6. Apigenin (LY 080400) purchased from Selleck.

产品安全说明书

P450 (e.g. CYP17)抑制剂选择性比较

生物活性

产品描述 Apigenin (NSC 83244, LY 080400)是一种有效的CYP2C9抑制剂,Ki为2 μM.。
特性 对CT-L的抑制比 kaempferol和myricetin更有效。
靶点
CYP2C9 [5]
2 μM(Ki)
体外研究

Apigenin通过与三磷酸腺苷(ATP)竞争而抑制PKC。Apigenin也降低TPA诱导的细胞蛋白的磷酸化水平,并抑制TPA诱导的c-jun和c-fos表达。Apigenin对转化的v-H-ras表型的NIH3T3细胞具有反转作用。[1]在中国仓鼠卵巢细胞中,Apigenin能够抑制硝基芘诱导的毒性基因引起的突变。在小鼠巨噬细胞中,Apigenin抑制LPS诱导的环氧合酶-2 和一氧化氮合成酶-2的活性和表达。Apigenin被报道具有抑制蛋白激酶C,分裂素激活的蛋白激酶(MAPK),C3HI小鼠胚胎成纤维细胞的转化以及v-Ha-ras-转化的NIH3T3细胞中下游致癌基因的活性。 Apigenin阻断过氧物酶体增殖调控的激酶(ERK),离体肝细胞中的MAPK。Apigenin可以下调Na+/Ca2+-交换器的表达,该蛋白是大鼠心肌细胞钙离子外排的一个重要蛋白。在表皮细胞和成纤维细胞中,Apigenin通过抑制p34 (cdc2)激酶活性引起G2/M and G0/G1可逆阻滞,并伴随p53蛋白质稳定性增加。在培养的人类内皮细胞中,Apigenin能够有效抑制TNFα诱导的细胞粘附分子-1的上调。在人类卵巢癌细胞中,Apigenin通过PI3K/Akt/p70S6K1和HDM2/p53信号通路抑制HIF-1α 和 VEGF表达[2]在人类角质细胞中,Apigenin通过抑制MAPK信号转导并降低API转录因子水平抑制分化。Apigenin也会抑制人类角质细胞的增殖。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse RAW264.7 cells NGPWZm9HfW6ldHnvckBie3OjeR?= NH:0W2dKdmirYnn0bY9vKG:oIFPPXFIheHKxdHXpckBmgHC{ZYPzbY9vKGmwIH3veZNmKFKDV{K2OE44KGOnbHzzMEBKSzVyPUCuOUDPxE1? NGCxeFAyPjB|OEWzOi=>
human H295R cells MV\GeY5kfGmxbjDhd5NigQ>? NU[xOJk6UW6qaXLpeIlwdiCxZjDhdo9u[XSjc3Wg[ZhxemW|c3XkJIlvKGi3bXHuJGgzQTWUIHPlcIx{NCCLQ{WwQVEh|ryP M3O1[|E5Pzd6OUS0
HEK293 FS cells Ml22SpVv[3Srb36gZZN{[Xl? MkH6TY5pcWKrdHnvckBw\iCQT2i0JIV5eHKnc4Pl[EBqdiCKRVuyPVMhTlNiY3XscJMh[XO|ZYPz[YQh[XNiSELPNkBxem:mdXP0bY9vKGK7IFiyU|IwXHm{L1zQU{Bie3OjeTygTWM2OD1zLkGzJO69VQ>? M2CwNlIxPzNzM{W3
human HeLa cells MYHGeY5kfGmxbjDhd5NigQ>? NVW2[pdyUW6qaXLpeIlwdiCxZjDNVnAyKHS{YX7z[oVkfGWmIHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDbN2heVFSFNDD0doFve3CxcoSgZpkhemGyaXSg[oltfHKjdHnvckBie3OjeTygT4k:Oi52IN88US=> MmG2NVk4OjV3N{i=
human MV4-11 cells NEfBOmVEgXSxdH;4bYNqfHliYYPzZZk> NEfxN5U4OiCq M3O4dWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1XPC1zMTDj[YxteyCqYYLic5JqdmdiRlzUN{BufXSjdHnvckBi\nSncjC3NkBpenNiYomgeIV1emG8b3zpeY0h[mG|ZXSgSZohS3mWb4igZ4VtdCC4aXHibYxqfHliYYPzZZktKEeLNUC9Nk45OSEQvF2= MYqyN|QyOTB5Mx?=
human mast cells M1P3cmZ2dmO2aX;uJIF{e2G7 NWW2c3RoUW6qaXLpeIlwdiCxZjDTXWshcW5iaIXtZY4hdWG|dDDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hdWG|dDDj[YxtKGSnZ4LhcpVt[XSrb36sJGVEPTB;MzFOwG0> MXuyNVM2PDhyMB?=
MDCK cells NXfpSWdFTnWwY4Tpc44h[XO|YYm= MkP4TY5pcWKrdHnvckBw\iCEQ2LQJIV5eHKnc4Pl[EBqdiCPRFPLJINmdGy|IIXzbY5oKEixZXPod5QhOzN|NEKgd5RicW6rbnesJGlEPTB;Mz6xJO69VQ>? MkezNlE{PTR6MEC=
human MDA-kb2 cells NVv3Z29PTnWwY4Tpc44h[XO|YYm= MWXBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGGwZILv[4VvKHKnY3XweI9zKGmwIHj1cYFvKE2GQT3rZlIh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDETHQucW6mdXPl[EBtfWOrZnXyZZNmKGGldHn2bZR6KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME21MlIh|ryP NG\3d4cyQTV7MkK0OS=>
MCF-7 MX cells M1vrRmZ2dmO2aX;uJIF{e2G7 M{GzXmlvcGmkaYTpc44hd2ZiQlPSVEBmgHC{ZYPz[YQhcW5iTVPGMVchVVhiY3XscJMhfXOrbnegTI9m[2i|dDCzN|M1OiC|dHHpcolv\yxiSVO1NF02NjlizszN NF\CWoUzOTN3NEiwNC=>
mouse RAW264.7 cells MVTGeY5kfGmxbjDhd5NigQ>? NWH4ZoIyOjRiaB?= NV3XN5ZuSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRibnn0dol1\SCjY3P1cZVt[XSrb36gZYZ1\XJiMkSgbJJ{KGK7IFfybYV{eyC{ZXHn[Y51KG2ndHjv[EwhUUN3ME22Mlch|ryP MkLFNVk4PzhyOE[=
human H9 cells MojYSpVv[3Srb36gZZN{[Xl? NHvVfY8{KGSjeYO= NF3YTXVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJUVZzIEPCJIlv\mWldHXkJIlvKGi3bXHuJGg6KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[geolz[WxicnXwcIlk[XSrb36gZYZ1\XJiMzDkZZl{KGK7IICyOEBidnSrZ3XuJINieHS3cnWgZZN{[XluIFXDOVA:QSEQvF2= NYC3OXRlQDF3OEG2OC=>
HEK293 cells MlPBSpVv[3Srb36gZZN{[Xl? NYflV45VOjRiaB?= MUHB[49vcXO2IHHjeIl3cXS7IHH0JI1wfXOnIGDQRXJo[W2vYTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHPvMYV5eHKnc4Ppcochf2m2aDDHZYw1KHKncH;yeIVzKH[nY4TvdkBi\nSncjCyOEBpenNiYomg[JVidC2udXPp[oVz[XOnIILldI9zfGW{IHHzd4F6NCCHQ{WwQVI1NjlizszN NFfzeoozPDl3NUi4PS=>
mouse 26-L5 cells NHPjZ4lRem:uaX\ldoF1cW:wIHHzd4F6 MmfsO|IhcA>? NYLWSZdbSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTCyOk1NPSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBGSzVyPUK1JO69VQ>? NGj0SHkyOjB{N{ezPS=>
human RS4:11 cells MWLDfZRwfG:6aXPpeJkh[XO|YYm= NX3VT5lwPzJiaB?= M4fYemN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHJUPDpzMTDj[YxteyCqYYLic5Jqdmdid3ns[EB1gXCnIF\MWFMh[W[2ZYKgO|IhcHK|IHL5JJRmfHKjen;sbZVuKGKjc3XkJGV7KEO7VH;4JINmdGxidnnhZoltcXS7IHHzd4F6NCCJSUWwQVI4NjlizszN MUiyN|QyOTB5Mx?=
mouse B16-BL6 cells Mn\OVJJwdGmoZYLheIlwdiCjc4PhfS=> MljUO|IhcA>? MoXURY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCEMU[tRmw3KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGVEPTB;M{GuOkDPxE1? MlnpNVIxOjd5M{m=
human H9 cells M2O2bGN6fG:2b4jpZ4l1gSCjc4PhfS=> NIj5Opk{KGSjeYO= NEjIbYhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJQSClZXzsd{Bi\nSncjCzJIRigXNuIFnDOVA:OzVizszN MkDQPFE2QDF4NB?=
human HT1080 cells NGLN[VhRem:uaX\ldoF1cW:wIHHzd4F6 NVi0N4I5PzJiaB?= MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiWMUC4NEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJRy=> MXqxNlAzPzd|OR?=
MDCK cells NHnjfJdEgXSxdH;4bYNqfHliYYPzZZk> NUHLWHFuS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTDT{Bk\WyuczDifUBOXFRiYYPzZZktKEOFNUC9N|kvPTlizszN NE\TVWoyQDZ2MEC0Ni=>
mouse L929 cells MkTxSpVv[3Srb36gZZN{[Xl? MmLDNVUhdWmwcx?= MVTQc5RmdnSrYYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iVF7GMYFteGijLXnu[JVk\WRiY4n0c5RwgGmlaYT5JI9nKG2xdYPlJGw6OjliY3XscJMh[XO|ZYPz[YQh[XNic4Xyeol3[WyrdImgdJJmcW6ldXLheIVlKG[xcjCxOUBucW6|IHLl[o9z\SCWTl\hcJBp[SCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkAzPCCqcoOgZpkh[3K7c4ThcEB3cW:uZYSgd5RicW6rbne= MlqyPVI5PzRzNR?=
human THP1 cells M{\1TGZ2dmO2aX;uJIF{e2G7 MWKyNEB2VQ>? MVGxJIg> NUCwdFdGUW6qaXLpeIlwdiCxZjDOU3gzKGmwIHj1cYFvKFSKUEGgZ4VtdHNiYYPz[ZN{\WRiYYOg[I94dnKnZ4XsZZRqd25ib3[gWHBCNWmwZIXj[YQhS0R|NjDtVm5CKGW6cILld5Nqd25iYYSgNlAhfU1iaX7jeYJifGWmIH\vdkAyKGi{IIDybY9zKHSxIGTQRUBkcGGubHXu[4UhdWWjc4Xy[YQh[W[2ZYKgNlQhcHK|IHL5JHJVNVCFUjDhcoFtgXOrcx?= MUmyN|c5PjV{MB?=
MDA-MB-231 cells NUPwWGYyTnWwY4Tpc44h[XO|YYm= MYm1JJVO MmHFTY5pcWKrdHnvckBw\iCSTVGtd5RqdXWuYYTl[EBPTi2tYYDwZWIhe2mpbnHsbY5oKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgOUB2VSCrbnP1ZoF1\WRiZn;yJFE3KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? NYjZTFM6OjVzOUC0OlY>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
MHC / MHC2A / MHC2B / MyoD ; 

PubMed: 29108230     


Apigenin increases total MHC, MHC2A, MHC2B and myoD expression in C2C12 cells. 

CXCR4; 

PubMed: 23743303     


(C) Apigenin suppressed CXCR4 levels in a dose-dependent manner. Cells were treated with the indicated concentrations of apigenin for 24 hours. CXCR4 expressions were indicated by anti-CXCR4 and anti-GAPDH western blotting analyses. (D) Apigenin suppressed CXCR4 levels in a time-dependent manner. Cells were treated with 40 μM apigenin for the indicated times, after which western blotting was performed as described above.

CDK1 / Cyclin B1 / p21 ; 

PubMed: 24009741     


Immunoblot analysis of G2/M phase regulators in cells treated with apigenin for 24 hr. CT: control.

29108230 23743303 24009741
Immunofluorescence
E-caherin / Vimentin ; 

PubMed: 27203387     


Typical images of immunofluorescent double staining for E-cadherin and Vimentin in Bel-7402 and PLC/PRF/5 cells. Each experiment was performed in triplicate.

Snail; 

PubMed: 27203387     


Typical immunofluorescence images of Snail in Bel-7402 and PLC/PRF/5 cells. Each experiment was performed in triplicate.

27203387
Growth inhibition assay
Cell viability; 

PubMed: 23224239     


Effect of apigenin on cell viability. Cells were treated with various concentrations of apigenin (2–30 μg/ml) for 24 h and cell viability was measured by MTT assay. Statistical significance: *p < 0.001.

23224239
体内研究 在卵清蛋白免疫的BALB/C小鼠中,Apigenin下调IL-4的产生。Apigenin通过抑制肿瘤细胞和内皮细胞的相互作用抑制黑色素瘤的肺转移。在雌性小鼠中,Apigenin引起子宫重量和整个子宫中雌激素受体(ER)-α浓度显著增加,通过雌激素受体β1抑制前列腺癌和乳腺癌细胞生长。Apigenin抑制前列腺移植瘤中IGF-I水平,增加在血液循环中结合IGF-I 蛋白的IGFBP-3含量。[2] Apigenin (12.5 毫克/千克)增加成年小鼠海马区齿状回区域细胞的增殖。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[5]

- 合并
  • Cell lines: WI-38, T-24, HT-1376 and PC-3 cells
  • Concentrations: 0, 1, 5, 10, 20, 30, 40, and 50 μg/ml
  • Incubation Time: 24 h
  • Method:

    To measure the effect of apigenin on cell viability, the WI-38, T-24, HT-1376 and PC-3 cells were seeded in 24-well plates (1 × 105 cells/well) for 16-18 h. The cells were then treated with or without various concentrations (0, 1, 5, 10, 20, 30, 40, and 50 μg/ml) of apigenin for 24 h. Each treatment was repeated 3 times. After the exposure period, the medium was removed and followed by washing the cells with PBS. The medium was then changed and incubated with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) solution (5 mg/ml)/well for 4 h. The medium was removed, and formazan was solubilised in isopropanol and measured spectrophotometrically at 563 nm. The percentage of viable cells was estimated by comparing them with the untreated control cells.


    (Only for Reference)
动物实验:

[6]

- 合并
  • Animal Models: heterozygous C57BL/TGN TRAMP mice
  • Dosages: 20 and 50 μg/mouse/day
  • Administration: p.o.
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 54 mg/mL (199.82 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 270.24
化学式

C15H10O5

CAS号 520-36-5
储存条件 粉状
溶于溶剂
别名 NSC 83244

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

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操作手册

如果有其他问题,请给我们留言。

  • * 必填项

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID