Acetylcysteine (N-acetylcysteine)
别名: N-acetylcysteine 中文名称：乙酰半胱氨酸
Acetylcysteine (N-acetyl-l-cysteine, NAC,N-acetylcysteine)是ROS抑制剂，拮抗多种蛋白酶体抑制剂的活性。它还是肿瘤坏死因子TNF的抑制剂，主要用作祛痰剂，可通过维持或恢复肝脏中谷胱甘肽的浓度来处理扑热息痛(对乙酰氨基酚过量)。Acetylcysteine(N-acetyl-l-cysteine) 通过抑制IκB kinases抑制TNF诱导的NF-κB活化。Acetylcysteine(N-acetyl-l-cysteine) 通过线粒体依赖性途径诱导凋亡并抑制铁死亡和病毒复制。在溶液中不稳定，请现配现用！
Acetylcysteine (N-acetylcysteine) Chemical Structure
CAS: 616-91-1
客户使用Selleck的Acetylcysteine (N-acetylcysteine)发表文献175篇
产品质控
常与Acetylcysteine (N-acetylcysteine)一起在实验中被使用的化合物
Aslam S, et al. Antimicrob Agents Chemother. 2007 Apr; 51(4): 1556–1558.
North TE, et al. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17315-20.
Acetylcysteine (N-acetylcysteine)相关产品
| 相关靶点 | TNF-α | 点击展开 |
|---|---|---|
| 相关化合物库 | 自噬化合物库 凋亡分子化合物库 铁死亡化合物库 细胞焦亡化合物库 线粒体靶向化合物库 | 点击展开 |
相关信号通路图
TNF-alpha抑制剂选择性比较
细胞实验数据示例
| 细胞系 | 实验类型 | 给药浓度 | 孵育时间 | 活性描述 | 文献信息 |
|---|---|---|---|---|---|
| HUVEC cells | Function assay | 2μM,4μM,8μM | 90min | HUVECs that were exposed with serum of P. falciparum and treated with NAC 2 µM，4 µM，8 µM. Supernatant from culture and lysed cells culture were measured for H2O2, GSH and MDA levels. | 21602763 |
| HUVEC cells | Function assay | 10μM | 2h | HUVECs were pretreated with 10 μM NAC for 2 hours, and then treated with 0.5 μM PCB 118. presentce of NAC for 48 hours. | 28592194 |
| NHBE | Cytotoxicity assay | 1 mM | 18 hrs | Cytoprotective activity in NHBE cells assessed as inhibition of tBHP-induced GSH depletion at 1 mM preincubated for 18 hrs followed by tBHP addition for 4 hrs by thiostar dye based fluorescence assay | 27031670 |
| HUVEC | Antioxidant assay | 5 mmol/L | 4 hrs | Antioxidant activity against H2O2-induced lipid accumulation in HUVEC cells assessed as reduction in MDA level at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs | 22841280 |
| HUVEC | Antioxidant assay | 5 mmol/L | 4 hrs | Antioxidant activity in HUVEC cells assessed as reduction in H2O2-induced GSH activity at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs | 22841280 |
| HUVEC | Cytotoxicity assay | 5 mmol/L | 4 hrs | Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as cell viability at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by MTT assay | 22841280 |
| HUVEC | Cytotoxicity assay | 5 mmol/L | 4 hrs | Inhibition of H2O2-induced cytotoxicity in HUVEC cells assessed as LDH release at 5 mmol/L incubated 4 hrs prior to H2O2 challenge measured after 12 hrs by LDH assay | 22841280 |
| SH-SY5Y | Neuroprotective assay | 5 mM | 4 hrs | Neuroprotective activity in H2O2-stimulated human SH-SY5Y cells assessed as upregulation of Bax expression at 5 mM incubated for 4 hrs prior to H2O2 challenge measured after 12 hrs by Western blotting analysis | 23403085 |
| SH-SY5Y | Neuroprotective assay | 5 mM | 4 hrs | Neuroprotective activity in H2O2-stimulated human SH-SY5Y cells assessed as downregulation of Bcl2 expression at 5 mM incubated for 4 hrs prior to H2O2 challenge measured after 12 hrs by Western blotting analysis | 23403085 |
| HT22 | Neuroprotective assay | 5 mM | 24 hrs | Neuroprotective activity against glutamate-induced cell death in mouse HT22 cells assessed as increase in cell viability at 5 mM after 24 hrs by MTT assay | 29122481 |
| HT22 | Neuroprotective assay | 50 uM | 10 to 12 hrs | Neuroprotective activity against glutamate-induced cell death in mouse HT22 cells assessed as reduction in apoptotic cells at 50 uM after 10 to 12 hrs by Annexin V-alexa 488/propidium iodide staining based flow cytometry | 29122481 |
| HL-7702 | Hepatoprotective assay | 10 uM | 24 hrs | Hepatoprotective activity against APAP-induced cell injury in human HL-7702 cells assessed as increase in survival rate at 10 uM pre-incubated for 24 hrs before APAP addition and measured 6 hrs post APAP challenge by MTT assay | 28729056 |
| PC12 | Cytotoxicity assay | 24 hrs | Inhibition of 6-hydroxydopamine induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level | 17158454 | |
| BL21 (DE3) | Function assay | 30 mins | Inhibition of hexahistidine-tagged IMP-7 (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using fluorocillin as substrate incubated for 30 mins by TECAN fluorescent plate reader analysis, IC50 = 20.7 μM. | 25815530 | |
| PC12 | Cytotoxicity assay | 24 hrs | Inhibition of hydrogen peroxide induced cytotoxicity in rat PC12 cells pretreated for 24 hrs assessed as elevation of intracellular glutathione level | 17158454 | |
| 点击查看更多细胞系数据 | |||||
生物活性
| 产品描述 | Acetylcysteine (N-acetyl-l-cysteine, NAC,N-acetylcysteine)是ROS抑制剂，拮抗多种蛋白酶体抑制剂的活性。它还是肿瘤坏死因子TNF的抑制剂，主要用作祛痰剂，可通过维持或恢复肝脏中谷胱甘肽的浓度来处理扑热息痛(对乙酰氨基酚过量)。Acetylcysteine(N-acetyl-l-cysteine) 通过抑制IκB kinases抑制TNF诱导的NF-κB活化。Acetylcysteine(N-acetyl-l-cysteine) 通过线粒体依赖性途径诱导凋亡并抑制铁死亡和病毒复制。在溶液中不稳定，请现配现用！ | ||||
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| 靶点 |
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| 体外研究(In Vitro) | ||||
| 体外研究活性 | N-acetylcysteine抑制c-Jun N末端激酶，P38 MAP激酶和氧化还原敏感激活蛋白1和核因子κB转录因子的活化，调节多种基因的表达。 N-acetylcysteine也能阻止细胞凋亡并通过激活细胞外信号调节激酶通路，是用于治疗某些退行性疾病有用的一个概念。N-acetylcysteine通过其还原活性直接调节几种蛋白质的活性。[1] 在没有其它营养支持的血清剥夺PC12细胞中，N-acetylcysteine可以防止细胞凋亡的DNA片段，并保持长期生存。 N-acetylcysteine还可以防止PC12细胞和交感神经元死亡。[2] 在大鼠和人的主动脉平滑肌细胞中，N-acetylcysteine剂量依赖性地减少存活率。[3] 在PC12细胞中，N-acetylcysteine激活Ras-外信号调节激酶（ERK）途径。N-acetylcysteine保护撤出营养支持诱发的神经元细胞免受死亡。 N-acetylcysteine增加存储在血管组织中一氧化氮（NO）的释放。 N-乙酰半胱氨酸预处理的PC12细胞干扰NGF-依赖的信号和神经突增生，并且有人提出，N-acetylcysteine干扰氧化还原敏感步骤。 [4] |
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| 体内研究(In Vivo) | ||
| 体内研究活性 |
N-acetylcysteine提高了12月龄SAMP8小鼠在T型迷宫电击回避范式和杠杆记者食欲任务的认知能力，不引起电机活动，激励非特异性作用以避免震动，或体重。[5] |
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|---|---|---|
| NCT Number | Recruitment | Conditions | Sponsor/Collaborators | Start Date | Phases |
|---|---|---|---|---|---|
| NCT06112834 | Not yet recruiting | Botulism |
California Department of Public Health |
June 2024 | Phase 2 |
| NCT04520139 | Not yet recruiting | Ovarian Cancer|Cognitive Impairment |
University of California Irvine|Jarrow Formulas Inc |
December 2023 | Phase 1|Phase 2 |
| NCT06039280 | Not yet recruiting | Hepatocellular Carcinoma |
Institute of Liver and Biliary Sciences India |
September 15 2023 | Not Applicable |
化学信息&溶解度
| 分子量 | 163.19 | 分子式 | C5H9NO3S |
| CAS号 | 616-91-1 | SDF | Download Acetylcysteine (N-acetylcysteine) SDF |
| Smiles | CC(=O)NC(CS)C(=O)O | ||
| 储存条件(自收到货起) | 3年-20°C 粉状 | 此产品性质不稳定，需现配现用!建议您购买分装规格，或者在收到货后进行分装。 | |
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体外溶解度 |
DMSO : 32 mg/mL ( (196.09 mM)； DMSO吸湿会降低化合物溶解度，请使用新开封DMSO) Water : 32 mg/mL Ethanol : 32 mg/mL |
摩尔浓度计算器 |
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体内溶解度 现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂 |
动物体内配方计算器 | ||||
实验计算
动物体内配方计算器（澄清溶液）
第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）
第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）
计算结果：
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；
体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。
体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。
注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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