Anisomycin

For research use only. Not for use in humans.

目录号:S7409 别名: Flagecidin, Wuningmeisu C 中文名称:茴香霉素

Anisomycin Chemical Structure

CAS No. 22862-76-6

Anisomycin (Flagecidin, Wuningmeisu C) 是一种从Streptomyces griseolus提取的抗菌性抗生素,抑制蛋白质合成,也是一种 JNK 激活剂。Anisomycin 可促进自噬与凋亡。

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客户使用Selleck生产的Anisomycin发表文献45篇:

产品安全说明书

JNK抑制剂选择性比较

生物活性

产品描述 Anisomycin (Flagecidin, Wuningmeisu C) 是一种从Streptomyces griseolus提取的抗菌性抗生素,抑制蛋白质合成,也是一种 JNK 激活剂。Anisomycin 可促进自噬与凋亡。
靶点
JNK [1]
体外研究

Anisomycin(3 μM)可降低MDA16和MDA-MB-468细胞中的蛋白质合成,减少MDA-MB-468细胞的集落形成。Anisomycin可促进MDA-MB-468细胞的凋亡,但不促进MDA16细胞的凋亡。在MDA-MB-468细胞中,anisomycin可激活JNK的磷酸化。[2] 在U251和U87细胞中,anisomycin(0.01-8μM)可时间依赖性和浓度依赖性地抑制细胞生长,其IC50值(48 h)分别为0.233和0.192 μM。Anisomycin(4 μM)可分别引起21.5%和25.3%的U251和U87细胞凋亡,并激活p38 MAPK和JNK活性,使ERK1/2失活。Anisomycin(4 μM)可时间依赖性地降低U251和U87细胞中PP2A/C亚基水平。[3] Anisomycin可浓度依赖性地抑制EAC细胞增殖。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 NUTGbYVqTnWwY4Tpc44h[XO|YYm= NX7sfJhCUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJJJmeXWrcnXkJJRwKHC{b3T1Z4Uh[3m2b4TvfIlkcXS7IHHnZYlve3RiSFXLNlk{KGOnbHzzMEBKSzVyPUCuNFLPxE1w MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjByNUKxN{c,OTZyMEWyNVM9N2F-
HeLa MnGzSpVv[3Srb36gZZN{[Xl? Mme1NVAhfU1? NGP4WohKdmirYnn0bY9vKG:oIITyZY5{dGG2aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMUCgeW0h[nliM{XTMY1mfGirb37pcoUhdWW2YXLvcIlkKGyjYnXsbY5oKHO2dXT5 M2\EelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MU[1NVM3Lz5zNUG2OVE{PjxxYU6=
HEK293 NYDIdlBQTnWwY4Tpc44h[XO|YYm= MlPCNVAxKHWP MmO1NVUhdWmwcx?= NYjHV|VWUW6lcnXhd4Uhd2ZiSl7LJJBpd3OyaH;yfYxifGmxbjDpckBWPTB2OEigeJJm[XSnZDD1cpRz[W6|ZnXjeIVlKEiHS{K5N{Bk\WyuczDheEAyODBidV2gZYZ1\XJiMUWgcYlvew>? M{e1[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{CyO|UxLz5zN{ewNlc2ODxxYU6=
HEK293 M1K4VGZ2dmO2aX;uJIF{e2G7 MoTuOVAhfU1? MYmxOUBucW6| MULJcoNz\WG|ZTDv[kBWPTB2OEitbY5lfWOnZDDKUmsheGixc4Doc5J6dGG2aX;uJIlvKHWwdILhcpNn\WO2ZXSgTGVMOjl|IHPlcIx{KGG2IEWwJJVOKGGodHXyJFE2KG2rboO= M1nmNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{CyO|UxLz5zN{ewNlc2ODxxYU6=
RAW264.7 MorSSpVv[3Srb36gZZN{[Xl? MlXsOUB2VQ>? NHX1VYs{OCCvaX7z MWfBZ5RqfmG2aX;uJI9nKHB|OF3BVGshcW5ibX;1d4UhWkGZMk[0Mlch[2WubIOgZZN{\XO|ZXSgZZMheGixc4Doc5J6dGG2aX;uJIF1KFSqckG4NE9VgXJzOEKgZZQhPSC3TTDh[pRmeiB|MDDtbY5{KGK7IGfld5Rmem5iYnzveJRqdmdiYX7hcJl{cXN? NETiUVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K5OFI5Pid-MkOyPVQzQDZ:L3G+
Sf9 NXXFcItFTnWwY4Tpc44h[XO|YYm= M1;VdVExKHWP MUO2JJRwKDJ2IHjy M2rzWGlv[3KnYYPlJI9nKEGWUDDs[ZZmdCCrbjDTdI9ld3C2ZYLhJIZzfWercHXy[IEhMG[jbHygZZJugXexcn2pJHNnQSClZXzsd{BifCBzMDD1UUBi\nSncjC2JJRwKDJ2IHjyJIJ6KGy3bXnu[ZNk\W62IHPlcIwhfmmjYnnsbZR6KGG|c3H5 NGHZUZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OjNzOUKvK|5EcEWPQly8M4E,
Sf9 NFrIcYFEgXSxdH;4bYNqfHliYYPzZZk> NFjkd|gyOCC3TR?= NIT6cow4OiCqch?= MXjDfZRwfG:6aXPpeJkh[WejaX7zeEBUeG:mb4D0[ZJiKG[{dXfpdIVz\GFiKH\hcIwh[XKveYfvdo0qKFOoOTDj[YxteyCjdDCxNEB2VSCjZoTldkA4OiCqcjDifUB1enmyYX6gZox2\SCmeXWg[ZhkdHW|aX;uJJRme3R? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NlMyQTJxJ{7DbGVOSkx:L3G+
VERO-E6 MVHGeY5kfGmxbjDhd5NigQ>? MX[0PEBpenN? NEDSc3ZF\XSncn3pcoF1cW:wIH;mJGlEPTBidnHseYV{KG[xcjDpcohq[mm2aX;uJI9nKFODUmOtR49XNTJiaX7keYNm\CCleYTveI95cWOrdImgc4YhXkWUTz3FOkBk\WyuczDh[pRmeiB2ODDoc5VzeyBiZYjwc5N2emVidH:gNE4xOSCPT1mgV2FTWyCFb2[tNkB3cXK3czDifUBpcWeqIHPvcpRmdnRiaX3h[4lv\yxiSVO1NF0xNjB7zszNMi=> NF7OdnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OjNzOUKvK|5EcEWPQly8M4E,
VERO-E6 MUfGeY5kfGmxbjDhd5NigQ>? MV:0PEBpenN? NV3WPWhNXG:6aXPpeJkhS0N3MDDh[4FqdnO2IG\FVm8uTTZiY3XscJMh\GW2ZYLtbY5m\CCjdDC0PEBpd3W{czDifUBpcWeqIHPvcpRmdnRiaX3h[4lv\yBqc3Ht[UBkd26maYTpc45{KGG|IELfUGV[KHerdHjveZQh\Xiyb4P1doUhfG9iMD6wNUBOV0liU1HSV{BEd1ZvMjD2bZJ2eyluIFPDOVA:OC5zzszNMi=> NIjnelU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1OjNzOUKvK|5EcEWPQly8M4E,
Vero E6 NUf6T4ViTnWwY4Tpc44h[XO|YYm= NHO1ZXRESzVyIHTleIVzdWmwYYTpc44h[XRiTV;JJFAvODB2IIXzbY5oKEOnbHzUbZRmei1iR3zvJEhEXEdrIHHzd4F6NCCyZYLmc5Ju\WRiMzDkZZl{KHCxc4StbY5n\WO2aX;uJIlvKF[ncn:gSVYh[2WubIOsJGNEPTB:MD6zPe69VS5? Mnm2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFI{OTl{Lze+R4hGVUKOPD;hQi=>
Vero E6 MWPGeY5kfGmxbjDhd5NigQ>? NHHPbYhESzVyIHTleIVzdWmwYYTpc44h[XRiTV;JJFAvODFidYPpcochS2WubGTpeIVzNSCJbH:gLGNVTyliYYPzZZktKHCncn\vdo1m\CB|IHThfZMheG:|dD3pcoZm[3Srb36gbY4hXmW{bzDFOkBk\WyuczygR2M2ODxyLkO5{txONg>? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NlMyQTJxJ{7DbGVOSkx:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-ERK / ERK / p-JNK / JNK / p-p38 / ATF3 ; 

PubMed: 17014422     


COS-1 cells were treated with anisomycin (50 ng/ml) for the indicated times and analysed by immunoblot with the indicated antibodies or by RT–PCR with primers specific to ATF3 or GAPDH.

PP2A / PP2C ; 

PubMed: 22684030     


U251 and U87 cells were non-treated (–) or treated with 4 μmol/L anisomycin for 6, 12, 24, or 48 h. PP2A C subunit level was detected by Western blotting. GAPDH served as loading control.

p-Keratin 20 / Keratin 20 / p-Keratin 18 / Keratin 18 / p-Keratin 8 / Keratin 8 / p-MK2 / p-hHSPB1 / hHSPB1; 

PubMed: 20724476     


HT29 cells were stimulated with 1 or 10 μg/ml anisomycin (Aniso) for 30 min. For p38 inhibition, cells were pretreated with 5 μm SB203580/5 μmSB202190 for 1 h followed by a 30-min stimulation with 10 μg/ml anisomycin. After treatment, the samples were analyzed by Western blotting using antibodies to the indicated antigens. GAPDH was used as a loading control. 

17014422 22684030 20724476
Immunofluorescence
p-Keratin 8 / p-Keratin 18 / p-Keratin 20; 

PubMed: 20724476     


HT29 cells grown on coverslips were left untreated or stimulated for 30 min with anisomycin, with or without pretreatment with 5 μm SB202190. Cells were fixed and stained using antibodies to the indicated antigens.

20724476
Growth inhibition assay
Cell viability; 

PubMed: 22684030     


U251 and U87 cells were separately treated with anisomycin at concentrations of 0, 0.004, 0.01, 0.04, 0.1, 0.4, 1, 2, 4, or 8 μmol/L for 48 h. Cell viability was analyzed using CCK-8 kit. Anisomycin started to suppress U251 and U87 cell growth at concentration of 0.01 μmol/L (cell viability is 75.3%±6.1% for U251 and 74.4%±4.3% for U87). At 8 μmol/L, the cell viability is only 18.4%±2.1% for U251 and 15.6%±1.3% for U87. Anisomycin inhibits U251 and U87 cell growth in a concentration-dependent manner. 

22684030
体内研究 瘤旁注射anisomycin(5 mg/kg)可显著抑制埃利希腹水癌(EAC)生长,EAC接种90天后的小鼠存活率为60%。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[2]
- 合并

JNK磷酸化:

以500000细胞/孔的密度接种细胞于6孔板中培养过夜。细胞用测试样品和作为空白对照的DMSO(终浓度1%,v/v)处理1 h。加入嘌呤霉素(终浓度为18 μM),继续培养10 min标记新生多肽链。背景值通过不加入嘌呤霉素培养细胞测定。然后在HBSS中洗涤细胞,通过刮取并离心收集细胞(300 g,离心5 min)。细胞重悬浮在含有磷酸酶抑制剂的0.5 mL 50 mM DTT中,95℃孵育10 min。随后样品经液氮快速冷冻,-20℃保存。样本(20–30 μg 蛋白/样本)被转移到PVDF膜。将膜封闭,并用anti-phospho-Thr183/Tyr185-JNK抗体在4℃孵育过夜。使用二抗标记一抗,用红外扫描仪检测。anti-phospho-JNK的荧光信号需经背景校正处理。
细胞实验:[4]
- 合并
  • Cell lines: 埃利希腹水癌(EAC)细胞
  • Concentrations: 500 ng/mL
  • Incubation Time: 48 h
  • Method: EAC细胞以10,000个细胞/孔/200 µL培养基的密度接种至96孔板中。细胞用不同浓度anisomycin处理48 h。Adriamycin(500 ng/mL)为阳性对照组。每孔加入0.5 mg/mL MTT,4 h 后,加入DMSO溶解MTT产物,Model 680酶标仪测定570 nm 处吸光值。
    (Only for Reference)
动物实验:[4]
- 合并
  • Animal Models: 雄性BALB/c小鼠
  • Dosages: 5 mg/kg
  • Administration: 瘤旁注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 41 mg/mL warmed (154.54 mM)
Ethanol 17 mg/mL warmed (64.07 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+corn oil
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 265.3
化学式

C14H19NO4

CAS号 22862-76-6
储存条件 粉状
溶于溶剂
别名 Flagecidin, Wuningmeisu C

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID