Home Apoptosis Caspase inhibitor Z-VAD-FMK 仅限科研使用

Z-VAD-FMK

别名: Z-VAD(OMe)-FMK

Z-VAD-FMK (Z-VAD(OMe)-FMK)是一种细胞渗透性的，不可逆泛caspase抑制剂，在THP.1 和 Jurkat T细胞中阻断细胞凋亡的所有特性。

Z-VAD-FMK Chemical Structure

Z-VAD-FMK Chemical Structure

CAS: 187389-52-2

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 3431.61 现货
1mg RMB 962.65 现货
5mg RMB 2858.31 现货
25mg RMB 7944.3 现货
1g RMB 80181.06 现货
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批次： 纯度： 99.79%
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常与Z-VAD-FMK一起在实验中被使用的化合物

Necrostatin-1

Z-VAD-FMK和Necrostatin-1可保护NP细胞免受压迫性死亡并恢复线粒体功能。

Chen S, et al. Biomed Res Int. 2020 Dec 8;2020:6976317.

Fer-1 (Ferrostatin-1)

Z-VAD-FMK和Ferrostatin-1治疗减轻了突触蛋白的损失，恢复了铁超载大鼠海马依赖和海马独立方式的认知功能。

Sripetchwandee J, et al. Life Sci. 2023 Jan 15;313:121269.

Y-27632 2HCl

Z-VAD-FMK和Y-27632 2HCl可阻断H2O2诱导的PC12细胞膜起泡和胞核凝聚。

Wang Y, et al. Front Mol Neurosci. 2017 Mar 14;10:75.

3-MA (3-Methyladenine)

Z-VAD-FMK可抑制脱氢木香内酯(DHL)诱导的自噬，而3-MA对DHL诱导的细胞凋亡无影响。

Peng Y, et al. Food Chem Toxicol. 2022 Dec;170:113453.

Erastin

在HS578T/MDAMB231细胞中，Erastin联合Z-VAD-FMK不能阻断Erastin诱导的细胞死亡。

Che C, et al. Cell Death Dis. 2022 Feb 14;13(2):150.

Z-VAD-FMK相关产品

相关信号通路图

Caspase Signaling Pathways

Caspase信号通路图

Caspase抑制剂选择性比较

细胞实验数据示例

细胞系 实验类型 给药浓度 孵育时间 活性描述 文献信息
Molt-3  Apoptosis Assay 50μM 2h reduces melatonin-induced apoptosis 23725013
Jurkat Cell Viability Assay 100-200μM 24h inhibits HaA4 induces apoptosis dependent of concentration  23732481
T98G Cell Viability Assay 1-100μM 24h improves cell viability cotreatment with TS 23769275
RKO Cell Viability Assay 10 μM 24h inhibits the decrease of cell viability caused by DAB 23758064
Ec-109 Apoptosis Assay 10 μM 48h represses PE-mediated Ec-109 cell apoptosis 23782641
HL-60 Apoptosis Assay 50µM 4h blocks the cleavage of caspase-3, -9, and PARP induced by 6s 23804706
U251 Apoptosis Assay 20μM 24h inhibits casticin induced G2/M phase arrest and apoptosis 23816816
HeLa  Apoptosis Assay 20μM 2h inhibits DMMP induced apoptosis 23863966
HL-60 Apoptosis Assay 100μM 24h inhibits apoptosis induced by abietane diterpenes  23865778
K562 Apoptosis Assay 20μM 48h inhibits apoptosis induced by 4-MU 23876826
THP-1  Apoptosis Assay 10-50μM 2h inhibits apoptosis induced by triptolide 23900299
CAL27 Apoptosis Assay 10 μM 2h suppresses Cur-NPs-reduced viability by up to 90% 23917396
COS-7  Function Assay 50µM 24h affects the processing of ATN1s with polyQ tracts 23933208
Jurkat Cell Viability Assay 25-100μM 6h inhibits z-FA-CMK-induced cell death 23933532
A431  Kinase Assay 40μM 2h blocks fisetin-induced cleavage of caspases and PARP 23800058
RAW264.7  Apoptosis Assay 10 μM 24h decreases the apoptosis induced by GA 23820203
Jurkat Cell Viability Assay 2μM 4h reverses growth inhibition and β-catenin decrease by HS-ASA 23896061
L929 Apoptosis Assay 1.25–5 μM  24h augments TNFα-induced necroptosis and autophagy 23941769
HL-60 Apoptosis Assay 50µM 1h inhibits apoptosis induced by BA145 23948751
CNE2 Apoptosis Assay 20μM 48h blocks LK-A-induced apoptosis 23985029
CNE1 Apoptosis Assay 20μM 48h blocks LK-A-induced apoptosis 23985029
IM-9 B Apoptosis Assay 20μM 2h blocks anti-CD80 and anti-CD86 antibody-induced apoptosis 24008628
EBV-transformed B cells Apoptosis Assay 20μM 2h blocks anti-CD80 and anti-CD86 antibody-induced apoptosis 24008628
MG-63 Apoptosis Assay 40μM 24h blocks the increased cleavage of caspase-3 and PARP induced by BBMD3  24025361
G292 Apoptosis Assay 40μM 24h blocks the increased cleavage of caspase-3 and PARP induced by BBMD3  24025361
KHOS  Apoptosis Assay 40μM 24h blocks the increased cleavage of caspase-3 and PARP induced by BBMD3  24025361
Nalm-6  Apoptosis Assay 20μM 4h inhibits caspase-8 and caspase-3 activation and PARP-1 cleavage 24039967
BGC-823 Apoptosis Assay 10 μM 24h reduces tetrandrine-induced apoptosis  24098511
UM-SCC-10A  Apoptosis Assay 50µM 2h reduces apoptosis induced by high-dose isoalantolactone 24098753
A549  Apoptosis Assay 2.5μM 24h decreases oridonin-induced autophagy as well and Loss of Δψm also occurred during autophagic process 24102522
RAW 264.7 Apoptosis Assay 20μM 18h increases LC3-II/β-actin ratio compared to ECTV-MOS treatment only 24116707
SCCVII  Apoptosis Assay 25μM 1h inhibits the cell killing after dasatinib 24126464
HeLa  Apoptosis Assay 10/20μM 24h inhibits the activity of the majority of the members of the caspase-family 24137266
SH-SY5Y  Apoptosis Assay 10μM 24h attenuates caspase activation and cell death induced by HNE-2DG 24145463
UD29a Apoptosis Assay 50μM 24h inhibits the cell death caused by NUT3 24190574
RPE Apoptosis Assay 100μM 48h partially inhibits the calpain-1 and -2 activation as well as the caspase activation 24202052
OS  Apoptosis Assay 20μM 4h inhibits the cell death caused by PDT 24204937
4T1 Cell Viability Assay 2.5-10μM 4h rescues the cytotoxicity of 4T1 cells induced by SPDT in a concentration dependent manner 24206161
HEC-1B Apoptosis Assay 20μM 1h reduces TP-induced apoptosis caspase-3 and caspase-9 24213358
RKO-HIPK2i Apoptosis Assay 40μM 6h inhibits apoptosis induced by chemotherapy plus ZnCl2 24228232
 U373  Apoptosis Assay 40μM 6h inhibits apoptosis induced by chemotherapy plus ZnCl2 24228232
ADF Apoptosis Assay 40μM 6h inhibits apoptosis induced by chemotherapy plus ZnCl2 24228232
MCC-2 Apoptosis Assay 20 μM 6h blocks induced by staurosporine apoptosis cell death 24262658
A549  Apoptosis Assay 100μM 48h suppresses the apoptosis caused by piperine  24272201
5637 Apoptosis Assay 20μM 1h reverses CM-induced cell death 24282433
MB49 Apoptosis Assay 20μM 1h reverses CM-induced cell death 24282433
PDL fibroblasts Kinase Assay 80μM  1h inhibits Caspase-3 activity  24314135
fetal rat lung fibroblasts  Kinase Assay 80μM  1h inhibits Caspase-3 activity  24314135
 HepG2  Apoptosis Assay 10μM 24h reduces vimentin cleavage caused by LPS 24325816
 KB Apoptosis Assay 50μM 24h inhibits Lico-A-induced caspase-3 and PARP activation 24337492
podocytes Apoptosis Assay 200μM 6h inhibits apoptosis induced by 3,4-DGE 24337777
HaCaT  Apoptosis Assay 100μM 1h blunts UVB-induced apoptosis in HaCaT cells 24356997
k1735 Apoptosis Assay 20 μm 4h inhibits Salmonella-induced apoptosis 24451116
SGC-7901 Apoptosis Assay 10μM 24h promotes the CGII-inhibited cell growth in SGC-7901 cells 24454488
CLL Apoptosis Assay 25μM 1h partially blocks MLN2238-induced cell apoptosis 24467634
Caki-1 Apoptosis Assay 20μM 1h inhibits SCP-induced apoptosis 24504681
A549 Apoptosis Assay 5μM 24h suppresses 6e (BK10040)-induced apoptosis  24529871
HT-29  Apoptosis Assay 50μM 48h blocks the apoptosis induced by kaempferol 24549175
 BV-2 Apoptosis Assay 20μM 2h suppresses UV-induced chromatin extrusion and dilation of the nuclear envelope 24558118
A549 Apoptosis Assay 50µM 2h prevents the hypodiploid DNA content phase induced by cephalochromin 24588135
HDPC Apoptosis Assay 50µM 24h inhibits NO-induced apoptosis 24634593
A375 Apoptosis Assay 20μM 24h reverses dicitrinone B-induced apoptosis 24699111
L02 Apoptosis Assay 20μM 1h prevents the cell apoptosis induced by MEHP 24706461
HCT116 Apoptosis Assay 20μM 24h inhibits the cell apoptotic induced by YLT205 24713812
IEC-6  Apoptosis Assay 10μM 24h prevents TcdA-induced caspase-3 cleavage and β-catenin degradation 24711571
HeLa  Apoptosis Assay 100μM 24h suppresses rate of cell death induced by overexpression of G59S or G71R p150glued  24722468
HeLa  Apoptosis Assay 50μM 72h prevents apoptosis induced by compounds 1–5 24754786
T cell Cell proliferation assay 0-100μM 72h IC50=70 μM, inhibits anti-CD3-induced T cell proliferation in PBMCs 24768707
U937  necroptosis Assay 10 μM 30 min induces necroptosis combine with TNF 24773756
LCC9  Cell Viability Assay 100μM 5d blocks cell death induced by combination L17 and chloroquine 24785256
AGS  Apoptosis Assay 20μM 12h reduces the induction of apoptosis in response to the EtOAc fraction in a dose-dependent manner 24789703
macrophage Cell Viability Assay 0-200μM 24h induces TNF- and Rip3-dependent necroptosis in macrophage 24799565
Caco-2 Apoptosis Assay 40 μM 4h prevents ST-7-induced ZO-1 changes and TER drop 24822183
SNU449  Apoptosis Assay 20μM 48h decreases miR-451-induced apoptotic 24841638
LOVO Apoptosis Assay 10 µM 1h restrains cell apoptosis induced by gefitinib plus decitabine 24874286
SW1116  Apoptosis Assay 10 µM 1h restrains cell apoptosis induced by gefitinib plus decitabine 24874286
HCT116 Apoptosis Assay 20μM 24h abrogates of TQ-induced apoptosi 24890449
P815  Apoptosis Assay 100 µM 12h inhibits virus-induced apoptosis 24923273
THP-1  Apoptosis Assay 10μM 1h reduces apoptosis induced by ALA-SDT  24923653
HeLa  Apoptosis Assay 40μM 24h inhibits the increased apoptosis induced by siRNF121 24928685
AGS Apoptosis Assay 50μM 24h abolishs β-lapachone-induced cell death and inhibited growth 25009698
C6 Apoptosis Assay 50μM 48h prevents the loss of cell viability caused by pregnenolone 25013479
MM.1S  Apoptosis Assay 100μM 1h efficiently prevents sorafenib-induced cell death combine with necrostatin-1 25037851
H929  Apoptosis Assay 100μM 1h partially blocks cell death caused by necrostatin-1 25037851
U266  Apoptosis Assay 100μM 1h partially blocks cell death caused by necrostatin-1 25037851
RPMI 8226 Apoptosis Assay 100μM 1h almost completely blocks cell death caused by necrostatin-1 25037851
IMR-32 Apoptosis Assay 40 μM  2h decrease in apoptotic cells compared to T-2 toxin 25084755
K562 Apoptosis Assay 0.1-1μM 1h inhibits cleavage of HSP90 in a dose-dependent manner 25119188
Jurkat Apoptosis Assay 20μM 24h partially inhibit cell death of Jurkat cells induced by 10058-F4 combined with VPA  25120723
Ebs Apoptosis Assay 10-100μM 24h  MMTS generation rate decreased as the concentration of z-VAD.fmk increased 25134817
EA.hy926 Apoptosis Assay 10μM 2h  inhibits apoptosis and facilitates autophagy in DENV2-infected EA.hy926 25138703
HUVECs Apoptosis Assay 10μM 2h  inhibits apoptosis and facilitates autophagy in DENV2-infected HUVECs 25138703
OS Apoptosis Assay 20/40μM 72h inhibits OS cell viability reduction by C6 ceramide 25152399
MCF-7 Apoptosis Assay 20μM 2h decreases KDR-siRNA-induced apoptosis 25182240
A549 Apoptosis Assay 10μM 3h reduces cell apoptosis caused by PQQ 25161699
L929 Apoptosis Assay 10μM 6h induces necroptosis with TNF 25195660
K562 Apoptosis Assay 50μM 4h inhibits Jac-A-induced cell apoptosis 25241619
H9c2 Apoptosis Assay 50μM 1h inhibits DOX-induced caspase 3 activation but not the loss of cells 25283819
769-P Apoptosis Assay 40μM 48h reduces the number of Annexin V-positive cells 25279191
Caki-1  Apoptosis Assay 40μM 48h reduces the number of Annexin V-positive cells 25279191
 NBL-W-S  Apoptosis Assay 50μM 1h fully rescues cell viability after GANT-61 treatment 25323222
A549 Apoptosis Assay 2.5-25μM 1h decreases the population of apoptotic cells depend on concentrations 25342427
A549 Apoptosis Assay 50μM 24h reverses ribosome biogenesis and apoptosis caused by Chijongdan 25349781
A375 Apoptosis Assay 30μM 2h prevents the drug-induced PARP cleavage 25376115
COS7 Apoptosis Assay 10μM 48h partially prevented FC101-induced cell death 25384025
COS7 Kinase Assay 10μM 24h increases caspase 3/7 activities 25384025
Cytotoxicity Assay Cell Viability Assay 20μM 48h prevent MHMD-induced cell death 25392116
L929-N Cytotoxicity Assay 20 μM 24h enhances death via autocrine TNFα production 25398540
L929-A  Cytotoxicity Assay 20 μM 24h inhibits TNFα-induced cell death 25398540
Primary hepatocytes  Apoptosis Assay 50μM 18h inhibits apoptosis of hepatocytes induced by Act D and TNF-α 25407538
INS-1 Apoptosis Assay 50μM 6h decreases the proportion of early apoptotic cells  25430897
A549  Apoptosis Assay 10μM 24h blocks DMAS-induced cleavage of caspase-3 and PARP and apoptotic cell death 25434989
AGS Apoptosis Assay 10μM 1h prevents curcumin-induced cleavage of caspase-3, -8, and -9 proteins 25492214
HL-60 Cytotoxicity Assay 100μM 1h  improves viability of TCNAs-treated cells 25502932
MKN28 Apoptosis Assay 10μM 30min inhibits TNF-α plus CHX-induced apoptosis 25513960
MDA-MB-231 Cytotoxicity Assay 10μM 1h augments cell death after CHUA treatment 25521501
DLD1 Apoptosis Assay 20 μM 1h partly reverses cell apoptosis caused by WSP1 25524246
MM Apoptosis Assay 50μM 20min partly inhibits SHK-induced cell death 25530098
Primary human placental cytotrophoblasts Apoptosis Assay 30μM 24h reverses the inhibition of 11β-HSD2 by triclosan  25642592
Neocortical Neuron Neurotoxicity Assay 100μM 1h antagonizes Hoiamide A-Induced Neurotoxicity 25675001
U87 Growth Inhibition Assay 50μM 1h recovers cell growth from TMZ treatment 25681668
A549 Apoptosis Assay 10 μM 24h promotes HBC-treated A549 cell survival & attenuates the cleaved PARP expression 25683568
MCF-7 Apoptosis Assay 10μM 2h inhibits the cell death induced by 3B 25722114
SGC-7901 Apoptosis Assay 20 μM 1h inhibits the cell death induced by oxaliplatin 25767076
HeLa Apoptosis Assay 10μM 48h declines the rate of apoptosis dramatically 25772545
A549 Apoptosis Assay 20 μM 24h reduces cell death caused by HFCP treatment 25794149
GC1a Apoptosis Assay 50μM 24h indicates a protective effect against etoposide 26169075
HGL5 Apoptosis Assay 50μM 24h indicates a protective effect against etoposide 26169075
HepG2 Apoptosis Assay 20μM 1h attenuated the apoptotic induction of III-10  26164795
BEL-7402 Apoptosis Assay 20μM 1h attenuated the apoptotic induction of III-10  26164795
CEF Kinase Assay 0,33,67,100μM 15min down-regulates PR enzyme activity to 40％ at 100μM 26102339
SP2/0 Apoptosis Assay 100μM 1h blocks the apoptosis of SP2/0 cells 26074732
HUVEC-2c Apoptosis Assay 50μM 6h decreased the ox-LDL-induced autophagy 26021729
U1 Apoptosis Assay 0-100μM 2h reduces drug-induced apoptosis and subsequent HIV-1 replication in a dose-dependent manner 25980942
ACH-2 Apoptosis Assay 0-200μM 2h reduces drug-induced apoptosis and subsequent HIV-1 replication in a dose-dependent manner 25980942
U1 Kinase Assay 100μM 2h inhibits caspase-3 25980942
A549/V16 Apoptosis Assay 50μM 1h represses TG/TM-induced apoptosis 25946033
SGN Apoptosis Assay 20mM 48h has no influence on AIF, calpain expression or cell apoptosis 25874633
HCT116  Apoptosis Assay 50μM 2h inhibits the cell death induced by DDVP 25868818
DTK-SME Apoptosis Assay 50μM 2h partially inhibits the apoptosis induced by bupivacaine 25843897
HL-60 Apoptosis Assay 50μM 48h reduces cell death caused by SKI-II treatment  25824043
U937 Apoptosis Assay 50μM 48h reduces cell death caused by SKI-II treatment  25824043
hMSC12 Apoptosis Assay 100μM 4d inhibits osteogenic culture-induced cell death and calcification 23657822
HM7  Apoptosis Assay 20μM  1h blocks apicularen A acetate-induced caspase-3 activation and PARP cleavage 23583412
Hep-2  Apoptosis Assay 20μM  24h alleviates the decrease of cell viability induced by silibinin 23580032
HSCs Apoptosis Assay 50μM 24h inhibits nilotinib-induced DNA damage indicated by PARP cleavage 23499874
HL-60  Cytotoxicity Assay 20μM  24h reduces the cytotoxic effect of Ery5 23494480
HA Apoptosis Assay 50μM 24h suppresses the cleavage of PARP and caspase-3, -7, and -9 induced by bortezomib/SAHA 23475956
C666-1 Apoptosis Assay 50μM 24h suppresses the cleavage of PARP and caspase-3, -7, and -9 induced by bortezomib/SAHA 23475956
Hepa1-6 Apoptosis Assay 50μM 2h inhibits curcumin and resveratrol-induced apoptosis 23446753
PLC/PRF/5c Apoptosis Assay 50μM 1h prevents apoptosis triggered by CIN 23438824
HCT116 Apoptosis Assay 100μM 1.5h inhibits Os-induced cell death 23744353
HCEC Function Assay 50μM 72h restores of the normal HCEC phenotype 23742011
Primary rat cerebral cortical neurons Apoptosis Assay 100μM 1h prevents Cd-induced apoptosis and cell death 23741317
MDA-MB-231  Apoptosis Assay 25μM 24h abrogates cytotoxicity and cleavage of caspase-3 and PARP induced by Rg3 25337544
NLRP3-Tet-on-MC/9  Apoptosis Assay 10-40μM 12h attenuates the cell death caused by CAPS-associated NLRP3 mutants in the Tet-on system 23703389
KNS42  Apoptosis Assay 50μM 1h reduces cell death and completely abolished caspase 3/7 activity in response to ABT-263/2DG/metformin combination 23691145
MCF-7  Apoptosis Assay 20μM  72h inhibits equol- and 4-OHT-mediated apoptosis 23675643
hCMEC/D3  Apoptosis Assay 25μM 72h reduces LtxA induced apoptosis 23665198
Jurkat Apoptosis Assay 12.5-50μM 1h dose dependently suppresses SPH-induced Par-4 cleavage, PARP cleavage, DNA fragmentation, and loss of viability 23442976
CNE-1 Apoptosis Assay 20μM  24h inhibits RAD001-induced cell death 23426850
HONE-1 Apoptosis Assay 20μM  24h inhibits RAD001-induced cell death 23426850
astrocytes cell Apoptosis Assay 40μM 6h reduces early apoptosis induced by staurosporine 23411778
U-937 Apoptosis Assay 10μM 30min prevents TNF-induced necroptosis 23410748
MDA-MB-231 Apoptosis Assay 100μM 1h inhibits sensitization to TRAIL upon MTDH down-regulation 23408429
HeLa  Apoptosis Assay 100μM 2h blocks JRS-15 Induces Apoptotic Cell Death 23344045
Ec109 Apoptosis Assay 10μM 6h blocks apoptotic combination of Tat-SmacN7 and radiation 23338568
H460  Apoptosis Assay 10μM 6h blocks apoptotic combination of Tat-SmacN7 and radiation 23338568
HeLa  Apoptosis Assay 50μM 1.5h abrogates Chlamydia-induced apoptosis 23303804
SK-HEP1 Apoptosis Assay 100μM 1h inhibits CrT1 activated caspase-3, -7, -8, -9, and poly(ADP-ribose) polymerase 23302650
QGY7701 Apoptosis Assay 25μM 1.5h inhibits accumulation of sub-G1 phase induced by DOX + quercetin 23240061
HepG2 Apoptosis Assay 20μM 30min inhibits the enhanced cell death by combined treatment of apigenin and TRAIL 23224239
U87  Apoptosis Assay 25μM 2h decreases isoliquiritigenin (ISL)-induced apoptotic cell death, but not necrotic cell death 23229626
HSC-2 Apoptosis Assay 25/50μM 1h inhibits SN-38-induced cytotoxicity 23155248
HSC-4 Apoptosis Assay 25/50μM 1h inhibits SN-38-induced cytotoxicity 23155248
CL-1 Apoptosis Assay 20μM 1h blocks OP449 induced cell death 23131782
MEL Apoptosis Assay 20μM 24h impaires P2X7-induced MEL cell apoptosis 23014887
Bel-7402  Caspase Activation Assay 50μM 2h inhibits PCE-induced anoikis 23008742
Eca-109 Apoptosis Assay 25μM 30min inhibits BJ-B11-induced cell death 23076967
MEL Apoptosis Assay 20μM 1h impaires P2X7-induced MEL cell apoptosis 23014887
Bel-7402  Apoptosis Assay 50μM 2h inhibits PCE-induced anoikis 23008742
L929 Function Assay 2.5μM 1h increases RIP1 expression and exacerbated TNFα-induced mitochondrial dysfunction and ROS production 23000518
RCC  Cell Viability Assay 100μM 24h recoveres the viability of cells exposed to 15d-PGJ2 22991494
NB2a/d1 Apoptosis Assay 100μM 72h attenuates staurosporine-induced caspase activity, PARP, and tau cleavage 22988541
T cell Growth Inhibition Assay 25-100μM 24h dose-dependently inhibited T cell proliferation mediated through the co-stimulation with anti-CD3 and anti-CD28 22982538
K562 Apoptosis Assay 100μM 1h blocks Abnobaviscum F-induced apoptosis 22972372
Jurkat  Apoptosis Assay 40μM 1h abolishes FasL-induced caspase activation and cell death 22942738
BGC-823 Apoptosis Assay 100μM 1h partially rescues cells against damage of daidzein 22926545
Hep3B Apoptosis Assay 50μM 1h blocks apoptosis induced by HEGCs 22923154
LLC-PK1 Apoptosis Assay 20μM 1h prevents cisplatin-induced degradation of Atg5, beclin-1, and Atg12 proteins 22896037
A549 Apoptosis Assay 50μM 1h blocks the BAI-induced apoptosis  22887215
SGC-7901  Apoptosis Assay 10μM 24h inhibits β,β-dimethylacrylshikonin-induced apoptosis  22848597
DM6 Apoptosis Assay 100μM 72h blocks both E2F-1 and E2Ftr-mediated cytotoxicity 22825328
MCF-7, MDA-MB-468, Caco-2 Growth Inhibition Assay 50μM 48h inhibits the cell growth inhibition induced by saponin 22800968
A2750  Apoptosis Assay 20μM 2h blocks caspase cleavage during helenalin treatment and reduces autophagic cell death 22784363
U87  Apoptosis Assay 20μM 24h reduces the apoptosis rate induced by PLAB 22778780
HT1080 Growth Inhibition Assay 50μM 7d inhibits the cell growth inhibition caused by combined treatment of DCA and OMP 22740984
A549  Apoptosis Assay 50μM 2h partially decreases sodium selenite-induced apoptosis 22721804
Primary OPC Apoptosis Assay 1μM 6h/24h reduces the percentage of cells in early- and late-apoptosis/necrosis 22707385
PMNs Apoptosis Assay 40μM 6h reversed the amount of cleaved caspase-3 to near vehicle levels 22692577
A549  Apoptosis Assay 50μM 1h prevents apoptosis induced by Baohuoside I 22687635
AGS Apoptosis Assay 20μM 12h inhibits the activation of pro-caspase-3 in response to the EtOAc fraction 22687398
shC9  Apoptosis Assay 10μM 16h blunts SHH expression in shC9 cells exposed to either PA or LPC 22641094
MDA-MB-231  Apoptosis Assay 20μM 4d reduces the cell apoptosis induced by metformin treatment was significantly 22593441
C6  Apoptosis Assay 10μM 24h blocks the suppressive effect of the peptide on viability 22588980
HL-60  Apoptosis Assay 100μM 24h inhibits the cell apoptosis selected (+)-menthyl β-(1→6)-diglucopyranoside 5 22546669
HL-60 Apoptosis Assay 10-80μM 4h inhibits TGHQ-induced cell apoptosis  22523229
BCC Apoptosis Assay 50μM 1h inhibits DATS-mediated growth inhibition 22519436
RAW 264.7 Apoptosis Assay 50/100μM 1h concentration-dependent inhibits DON-induced rRNA cleavage 22491426
K562 Apoptosis Assay 25μM 2h prevents apoptosis induced by co-treatment with amurensin G and TRAIL 22483777
SGC-7901 Apoptosis Assay 20 μM  2h attenuates H2O2 or TNF α-induced cell apoptosis as well as caspase-3 activity  22471589
PC3 Apoptosis Assay 10μM 4h counters flavocoxid-induced caspase-related apoptosis 22471974
SMMC-7721 Apoptosis Assay 50μM 48h attenuates oTR-induced apoptosis 22465833
HeLa  Apoptosis Assay 50 μM 4/8h inhibits STS-induced late-phase apoptotic events 22460504
HeLa  Apoptosis Assay 50 μM 1h suppresses the FRAP-induced accumulation of annexin V positive cells 22449440
T47D  Apoptosis Assay 100μM 1h blocks the generation of E-cad/CTF2 by STS 22401168
HeLa  Apoptosis Assay 30μM 4h  increases the general cell viability 48 h after photodynamic treatment  22394248
HCC Apoptosis Assay 20 μM  2h attenuates the DHA induced activation of PARP 22342732
mESCs Apoptosis Assay 2.5μM 2h inhibits the NaF-mediated caspase activation 22285274
EMT-6  Cytotoxicity Assay 100μM 1h partially blocked cell death induced by siramesine 22251921
MCF7 Apoptosis Assay 50 μM 1h inhibits PA activated caspase-3, -9, and poly(ADP-ribose) polymerase 22223345
K562 Apoptosis Assay 20 μM  48h blocks lapatinib-induced inhibition of viability and apoptosis induction 22216158
Molt4-hyg Apoptosis Assay 10μM 0.5h blocks farnesol-induced caspase-3-like activity 26275811
HeLa Apoptosis Assay 10μM 0.5h inhibits apoptosis induced by the combined treatment with gomisin N and TRAIL 22179661
Jurkat T Apoptosis Assay 30μM 0.5h blocks the ziram-induced apoptosis 22159898
Neutrophil Apoptosis Assay 20μM 0.5h attenuates the pro-apoptotic effect of MaR1 26196844
HCT116 Apoptosis Assay 50 μM 2h reverses synergistic apoptosis effects of celecoxib and NPC-16  22159752
MDA-MB-231  Apoptosis Assay 2.5-7.5μM 2h inhibits the cell death of MDA-MB-231 cells induced by SDT in a concentration dependent manner 22115526
LNCaP Apoptosis Assay 40μM 2h inhibits butein induced cell apoptosis 22114764
MB231 Apoptosis Assay 100μM 1h abrogates the induction of cell death by WEE1 inhibition, TRAIL treatment, and the combination 22112940
HCC38  Apoptosis Assay 100μM 1h abrogates the induction of cell death by WEE1 inhibition, TRAIL treatment, and the combination 22112940
MDA-MB231 Apoptosis Assay 50 μM 24h abrogates curcumin-induced cell death 22101335
LNCaP Apoptosis Assay 50 μM 24h abrogates curcumin-induced cell death 22101335
HCT116 Apoptosis Assay 50 μM 24h abrogates curcumin-induced cell death 22101335
Ishikawa  Apoptosis Assay 25μM 24h reduces cell death and apoptosis induced by Baf A1 22088918
 YD-8  Apoptosis Assay 100μM 1h inhibits PLEO-induced apoptosis 22086183
eosinophil  Apoptosis Assay 90μM 24h inhibits apoptosis induced by Fas antibody  22079334
L929 Growth Inhibition Assay 2.5μM 24h inhibits the expression of p-p38 and NF-κB to augment TNFα-induced necroptosis and autophagy 22027097
YD-8  Apoptosis Assay 100μM 1h blocks the GS-HCl-induced apoptosis 22020078
HBx Apoptosis Assay 25μM 48h reduced cell death induced by 3-MA 22020078
U937  Apoptosis Assay 50 μM 1h inhibits HF-induced apoptosis  21998731
HL60 Apoptosis Assay 40μM 40min blocks BNDC compounds induce exposure of phosphatidylserine and DNA fragmentation 21983296
M-14 Apoptosis Assay 25μM 0.5h  inhibits both the crude extract- and compound-induced apoptosis 21954959
SK-BR-3 Apoptosis Assay 50 μM 2h blocks apoptotic DNA fragmentation induced by combination of SM-164 and radiation 21901386
MDA-MB-468 Apoptosis Assay 50 μM 2h blocks apoptotic DNA fragmentation induced by combination of SM-164 and radiation 21901386
MIA-PaCa-2 Apoptosis Assay 24h blocks cleavage of caspase-3 induced by both A-443654 and paclitaxel 24510992
primary MEFs Function Assay 12h  increases etoposide-induced mitochondrial membrane depolarization 22613767
3T9 MEFs Function Assay 16h increases cytochrome c release after etoposide-treatment 22613767
3T9 MEFs Function Assay 18h upregulates initiator caspase-9 but downregulates effector caspases after etoposide treatment 22613767
点击查看更多细胞系数据

生物活性

产品描述 Z-VAD-FMK (Z-VAD(OMe)-FMK)是一种细胞渗透性的，不可逆泛caspase抑制剂，在THP.1 和 Jurkat T细胞中阻断细胞凋亡的所有特性。
特性 Z-VAD-FMK是研究细胞凋亡的一种关键化合物。
靶点
Pan-caspase [1]
(THP.1, Jurkat T-cells)
体外研究(In Vitro)
体外研究活性

Z-VAD-FMK (10 μM)作用于THP.1细胞，抑制细胞凋亡。Z-VAD-FMK(10 μM)作用于THP.1细胞裂解液，抑制PARP蛋白酶活性的激活。Z-VAD-FMK (10 μM)作用于完整THP.1和 Jurkat细胞，抑制CPP32加工。[1]Z-VAD-FMK(50 μM)与Camptothecin共处理HL60细胞，废除Camptothecin处理的细胞凋亡形态。Z-VAD-FMK(50 μM)作用于HL60细胞，抑制Camptothecin诱导的DNA分裂。[2]Z-VAD-FMK (50 μM)作用于S2细胞，提高转染细胞的生存百分数从26％提高到63％。[3]Z-VAD-FMK(>100 μM)作用于人类嗜中性粒细胞，增强TNFα-诱导的中性粒细胞凋亡，较低浓度(1-30 μM)时完全抑制TNFα刺激的细胞凋亡。[4]Z-VAD-FMK (10 mM) 作用于早期角膜基质细胞，抑制细胞凋亡。[5]
细胞实验 细胞系 颗粒细胞GC1a, HGL5, COV434
浓度 50 μM
孵育时间 48 h
方法

为了确定Z-VAD-FMK的效力，在常氧条件下，用etoposide (50 μg/ml) 和/或 Z-VAD-FMK (50 μM)对三种人源颗粒细胞系(GC1a, HGL5, COV434)进行处理，处理48小时。为了模拟卵巢片段抑制后的缺氧状态，用低氧（1% O2）状态下，用无血清培养基培养细胞，处理以Z-VAD-FMK。用WST-1试验检测细胞的代谢活性。通过FACS检测细胞活力，RT-qPCR和WB检测凋亡相关分子的表达。
实验图片 检测方法 检测指标 实验图片 PMID
Western blot IRF-1 / p-STAT1 / cleaved PARP1 / cleaved-caspase3 Beclin1 / Atg5 / Atg7 / LC-3I / LC-3II p23 / Hsp90 / hTRET / MMP2 cyto C 27191889
Growth inhibition assay Cell viability 12522090
Immunofluorescence cytochrome c Smac/DIABLO Endo G AIF 11726499
ELISA IL-1β 26254306
体内研究(In Vivo)
体内研究活性

有研究表明，体内Z-VAD-FMK的给药不具有毒性，在动物模型中可抑制凋亡。在小鼠中，腹腔注射HK-GBS可引起早产，而预处理以Z-VAD-FMK可延迟早产现象。在OVA-sensitized的小鼠中，z-VAD-fmk的处理可抑制过敏原诱导的白细胞浸润。在OVA致敏前全身性注射z-VAD-fmk可减少OVA-sensitized/challenged小鼠中炎性细胞的积累、黏液的高分泌以及Th2细胞因子的释放。处理以z-VAD-fmk可阻止晶状体上皮细胞、角质细胞的终末分化；单核细胞向巨噬细胞的分化；以及红系造血祖细胞的分化。z-VAD-fmk可减缓过敏原诱导的呼吸道炎症以及高敏性、阻止后续T细胞的激活[7]。
动物实验 Animal Models CD1小鼠
Dosages 10 mg/kg
Administration i.p.

化学信息&溶解度

分子量 467.49 分子式

C22H30FN3O7
CAS号 187389-52-2 SDF Download Z-VAD-FMK SDF
Smiles CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1
储存条件(自收到货起)

体外溶解度
批次：

DMSO : 93 mg/mL ( (198.93 mM)； DMSO吸湿会降低化合物溶解度，请使用新开封DMSO)

Ethanol : 2 mg/mL

Water : Insoluble

摩尔浓度计算器

体内溶解度
批次：

现配现用，请按从左到右的顺序依次添加，澄清后再加入下一溶剂

 动物体内配方计算器

实验计算

摩尔浓度计算器

质量 浓度 体积 分子量

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

mg/kg g μL 只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH2O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

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常见问题及建议解决方法

问题 1:
Can you suggest an in vivo formulation of S7023 for animal studies?

回答：
For in vivo study of S7023, The IP formulation is 2% DMSO+35 % PEG 300+2% Tween 80+ddH2O up to 6 mg/ml. The gavage formula is 1%CMC-Na up to 30 mg/ml (suspensions).

问题 2:
What is difference between S7023 & S8102?

回答：
Unlike S7023, S8102 does not require pretreatment with esterase for in vitro studies. This information was cited from the article: https://www.ncbi.nlm.nih.gov/pubmed/16973565.

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