Vismodegib (GDC-0449)

For research use only. Not for use in humans.

目录号:S1082 中文名称:维莫德吉

Vismodegib (GDC-0449) Chemical Structure

CAS No. 879085-55-9

Vismodegib (GDC-0449)是一种新型有效的,特异性hedgehog抑制剂,无细胞试验中IC50为3 nM,也会抑制P-gp,IC50为3.0μM。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1179.52 现货
RMB 730.63 现货
RMB 3046.91 现货
RMB 8763.3 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Vismodegib (GDC-0449)发表文献164篇:

产品安全说明书

Hedgehog/Smoothened抑制剂选择性比较

生物活性

产品描述 Vismodegib (GDC-0449)是一种新型有效的,特异性hedgehog抑制剂,无细胞试验中IC50为3 nM,也会抑制P-gp,IC50为3.0μM。
靶点
Hedgehog [1]
(Cell-free assay)
3 nM
体外研究

GDC-0449有效抑制hedgehog通路,是一种新型及特定合成的小分子抑制剂,IC50为3nM。[1] GDC-0449作用于Hedgehog信号通路,阻断Hedgehog配位体,即细胞表面受体PTCH 及SMO的活性,从而阻断Hedgehog信号通路。在组织生长和修复方面,Hedgehog信号通路意义重大。Hedgehog通路信号的异常激活,及不受控制的细胞增殖,可能与Hedgehog配位体,即细胞表面受体PTCH 及SMO的突变有关。在体外,GDC-0449在不抑制胰脏细胞增殖的前提下阻止原代胰脏移植物的生长,这项结果最近已经应用到临床。GDC-0449也抑制ABCG2, P-糖蛋白, 及和MDR有关联的重要MRP1 ABC载体。GDC-0449也阻断其他多种ABC载体。ABC载体属于一个膜蛋白家族,它的过量表达和耐药性有关,这也是治疗癌症的一个重大障碍。GDC-0449强抑制两种ABC载体,ABCG2/BCRP和ABCB1/P-糖蛋白,也温和抑制ABCC1/MRP1。在ABCG2过量表达的HEK293细胞中, GDC-0449可以提高荧光ABCG2底物BODIPY-哌唑嗪的保持力,也可用米托蒽醌再次处理这些细胞,产生抗肿瘤的ABCG2底物。实验使Madin-Darby犬的肾脏细胞中过量表达P糖蛋白或者MRP1,GDC-0449 提高了钙黄绿素-AM的保持力,然后载用秋水仙素处理这些细胞。另外,用米托蒽醌,托泊替康,SN-38处理的过量表达ABCG2的人类非小细胞肺癌细胞NCI-H460/par和NCI-H460/MX20,用GDC-0449再次处理。GDC-0449 作用于ABCG2 和 Pgp 的IC50值分别为1.4μM和 3.0 μM。[2]GDC-0449 改变细胞内Ca2+ 平衡,且作用于抗cisplatin的肺癌细胞, 抑制细胞生长。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
IGROV-1 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTBwMEeyOFgh|ryP NGXkdZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
HCE-T MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfUTWM2OD1zLkOyNlQ4KM7:TR?= NUfMd|k5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
D-542MG NV\2UpJXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmSwTWM2OD1zLki2O|M4KM7:TR?= Ml;TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
23132-87 NITKZnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2izcmlEPTB;ND60NFE1PyEQvF2= MkLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
HDLM-2 MmKzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;yd4hUUUN3ME24MlA1PzZ4IN88US=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
ACN NUHiNVZIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;aTWM2OD16LkWwNVA6KM7:TR?= NEPmVoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
HuO-3N1 M{HueGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{n2TWlEPTB;OT62NFExQCEQvF2= M3\aZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
BHT-101 NIe3fFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvSPXVwUUN3ME2xNU4{QCEQvF2= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
KYSE-150 M3\jc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPNTWM2OD1zMT61PFQyKM7:TR?= NILNclk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
MC-IXC NXW3XYVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LabmlEPTB;MUKuNlI6OiEQvF2= M{e1ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
D-423MG MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYX5OVFjUUN3ME2xNk44PjV5IN88US=> M4j5OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
NY MkjXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzCTWM2OD1zND64PVA{KM7:TR?= M1\XNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
HOS M2HuN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HmcmlEPTB;MUWuOlcyQSEQvF2= NG\XfXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
NB7 NWS1VWRDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTF3Lki5NUDPxE1? M4[yNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
DMS-273 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVT2W3NHUUN3ME2xOk43PzF|IN88US=> NF\CZYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
MDA-MB-361 MoLVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1ywSGlEPTB;MUeuNlcyOSEQvF2= NUTSbIlvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
DU-145 Mki4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTF6LkOyJO69VQ>? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
NCI-H82 NUDoe5hlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnpZWFKSzVyPUG5Mlg{QDZizszN MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
NCI-SNU-1 MlrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXXJR|UxRTJyLkCxPVYh|ryP MkHiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
GCT MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MorLTWM2OD1{MD64PFI1KM7:TR?= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
C2BBe1 NYr5fpg{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTJzLkGwOVgh|ryP MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
LB2241-RCC M{juTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfDVWhKSzVyPUKxMlg1PDFizszN NW\HdXRRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
COLO-829 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nafGlEPTB;MkKuNVg4OSEQvF2= NVrHT3pNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
EW-11 MkHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTJ{LkiwNlIh|ryP NXLkcpVuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
NCI-H526 NXrySXZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTM[WFKSzVyPUKzMlQ4OTdizszN NF7oNI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
SF295 NVXqeoRuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkTlTWM2OD1{ND6wNlUzKM7:TR?= MoLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
D-566MG MkHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PiN2lEPTB;MkWuNlk1OyEQvF2= M1fhT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
8505C MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTLSGVKSzVyPUK1MlY{OzFizszN NIjzbmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
HT-29 NIjIcIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTJ4LkC0N|Eh|ryP M1TU[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
NBsusSR M1LzRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3ZTWM2OD1{Nj64NFA3KM7:TR?= M4jEWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
BV-173 NILZfI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3[0UGlEPTB;MkiuN|E5OiEQvF2= NFrmTXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
CTB-1 M4HEWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3rRN2lEPTB;M{CuNVA{OSEQvF2= NFjURZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
JAR NG\TcmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnScXRKSzVyPUOyMlU{PzFizszN Mn7oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
CAMA-1 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\SNWRrUUN3ME2zN{41PjF3IN88US=> MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
CAL-51 NEDNcHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTN2LkexO|Yh|ryP NXPQT3RMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
A172 NVjlfFZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlPETWM2OD1|Nz60PVIyKM7:TR?= M33LN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
QIMR-WIL NUL1PINHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;PO2lEPTB;M{iuNFcxQCEQvF2= NETsTHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
AsPC-1 M4Xsb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTN6LkS2OVEh|ryP MlfpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
MKN7 NEnSZmdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnwfFVKSzVyPUO5MlAxPzlizszN MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
ONS-76 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTR|LkOwOVch|ryP MljSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
RS4-11 M2PSfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mk\jTWM2OD12ND6wO|UzKM7:TR?= MofxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
NOS-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYTuPHkyUUN3ME20OE43ODNzIN88US=> NF7Vc2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
A101D MkK2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfJTIhSUUN3ME20OE45ODJ|IN88US=> MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
HCC1806 M4jTTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTR4LkGxOFgh|ryP NV\kdGNIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
CAL-27 NVXITW8{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTR5LkeyOFYh|ryP NXLpT5pmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
BT-549 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX;lZXc2UUN3ME20PE42OzF3IN88US=> NGOwUYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
LCLC-97TM1 M1z0VWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnHiTWM2OD12OT6yOFE{KM7:TR?= NIjwZlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
A4-Fuk NHPVVWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Moe4TWM2OD12OT64OFkh|ryP NUC4WJpiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
OVCAR-4 NGPnN3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTVyLkC2NFEh|ryP M1fVdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
HD-MY-Z NH7PS|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\1XWlEPTB;NUCuO|c3PCEQvF2= NYjpN|diRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
NCI-H292 Mm[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHxc3RKSzVyPUWwMlg4PThizszN MnnkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
Sk-ChA-1  MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3aNE4zPeLCk{WwJO69VQ>? NGXJbGQ4OiCq MoSxTWM2OD15ND61OOKyOi53ON88US=> NHH3bFA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe0NlQ5Oid-MkW3OFI1QDJ:L3G+
Mz-ChA-1 M{i3eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXlNE4zPeLCk{WwJO69VQ>? MWm3NkBp NYmwb2dzUUN3ME21OE46P8LzMz60Oe69VQ>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd2MkS4Nkc,OjV5NEK0PFI9N2F-
Smo-WT Mk[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHPpNndKSzVywrDv[kAyPMLibl2= NEP6[JU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK5NVExPCd-MkSyPVEyODR:L3G+
Smo-D473H  M2HUN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17nZWlEPTEEoH;mJFcvOcLizszN MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ7MUGwOEc,OjR{OUGxNFQ9N2F-
K562 NXzGOVU6TnWwY4Tpc44hSXO|YYm= NH;YPXEyOCEQvF2= NE\1TGc4OiCq NFvieYNz\WS3Y3XzJJRp\SCneIDy[ZN{cW:wIH;mJGdtcTIEoB?= NGDxUYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OxPVgzPCd-MkOzNVk5OjR:L3G+
T315I BCR-ABL BaF3 MmG1SpVv[3Srb36gRZN{[Xl? NVHNdmRrOTBizszN NFzLcJM4OiCq NUf4RphQemWmdXPld{B1cGViZYjwdoV{e2mxbjDv[kBIdGlzwrC= MlPMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MUm4NlQoRjJ|M{G5PFI1RC:jPh?=
TF-1 BCR-ABL NVLqNm1KTnWwY4Tpc44hSXO|YYm= Mn3QNVAh|ryP MYi3NkBp MVvy[YR2[2W|IITo[UBmgHC{ZYPzbY9vKG:oIFfsbVHDqA>? M{f5dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{G5PFI1Lz5{M{OxPVgzPDxxYU6=
Shh-Light 2 NUP0V2x5TnWwY4Tpc44h[XO|YYm= MlG0NkBl[Xm| M17MTmFvfGGpb37pd5Qh[WO2aY\peJkh[XRiU33vJIlvKG2xdYPlJHNpcC2OaXfoeEAzKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gV4hpNWmwZIXj[YQhT2yrMT3y[ZBwenSncjDhZ5Rqfmm2eTDh[pRmeiB{IHThfZMh[nliZIXhcE1tfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgcYV1cG:mLDDJR|UxKD1iMD6wNFE2KM7:TT6= M{DOR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[zOVIzLz5{M{C2N|UzOjxxYU6=
NIH/3T3 NEPRenBHfW6ldHnvckBie3OjeR?= NEntOXNKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGK7IFfsbU1lfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKgZZN{[XluIFnDOVAhRSByLkCwNlMh|ryPLh?= M2\QV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUiwNFEzLz5{N{G4NFAyOjxxYU6=
NIH/3T3 Light2 NHjGTXBHfW6ldHnvckBie3OjeR?= NGfNb25KdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIF7JTE8{XDNiTHnnbJQzKGOnbHzzJIJ6KEeuaT3seYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUCgQUAxNjByMkOg{txONg>? M2PjO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki4Nlc5Lz5{OE[4PFI4QDxxYU6=
Light2 M{PKSmZ2dmO2aX;uJIF{e2G7 NUfmc|c{PDhiaILz MXXJcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6KGmwIH3veZNmKEyrZ3j0NkBk\WyuczDh[pRmeiB2ODDodpMh[nliR3zpMYx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMECyOFYh|ryPLh?= NWTSS|VjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3N|k4OTRpPkK5O|M6PzF2PD;hQi=>
HEPM M4rMR2Z2dmO2aX;uJIF{e2G7 NIjISWdKdmirYnn0bY9vKG:oIGPITEBqdiCqdX3hckBJTVCPIHPlcIx{KGK7IFfsbU1tfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnOTEA:KDBwMECyPEDPxE1w NXqyU4x[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3NVYzQTZpPkG5O|E3Ojl4PD;hQi=>
Shh Light2 NXi1cYVRTnWwY4Tpc44h[XO|YYm= M1WwfGlvcGmkaYTpc44hd2ZiU1jIJIlvKG2xdYPlJHNpcCCOaXfoeFIh[2WubIOgZpkhT0yLLYLld5BwdnOrdnWg[olz\W[ueTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUCgQUAxNjByMzFOwG0v MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTNyOUC4NEc,OTl|MEmwPFA9N2F-
HEPM NI\jNIdHfW6ldHnvckBie3OjeR?= M{PoZmlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIHnuJIh2dWGwIFjFVG0h[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIILlZ4VxfG:{LX3l[IlifGWmIFfsbVEhdVKQQTDlfJBz\XO|aX;uJIJ6KHKncH;yeIVzKGenbnWgZZN{[XluIFnOTEA:KDBwMECzJO69VS5? NGLQdmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3OVc1OSd-MkC4O|U4PDF:L3G+
C3H10T1/2 M3P5WmZ2dmO2aX;uJIF{e2G7 MWmyNEBpenN? NUfqXGVFUW6qaXLpeIlwdiCxZjDTcY8hcW5ibX;1d4UhSzOKMUDUNU8zKGOnbHzzJJV{cW6pIHj1cYFvKHKnY3;tZolv[W62IGPITEBie3Onc4Pl[EBieyCnZn\lZ5Qhd25iU13PM3NJUCC2cnHud4lmdnRidILhcpNkemmydHnvcoFtKGGldHn2ZZRqd25iYX\0[ZIhOjBiaILzJIJ6KEeuaT3seYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7LDDJR|UxKD1iMD6wNFUh|ryPLh?= NFjFZlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFQ{Pid-MkS5NFA1OzZ:L3G+
NIH/3T3 MmPjSpVv[3Srb36gZZN{[Xl? M3fqc|I1KGi{cx?= NIDpXnZKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIF7JTE8{XDNiY3XscJMhdWWjc4Xy[YQh[W[2ZYKgNlQhcHK|IHL5JGdtcS2mdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMEC1JO69VS5? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzhzMEW5NUc,Ojd6MUC1PVE9N2F-
NIH3T3 M1y5W2Z2dmO2aX;uJIF{e2G7 NHfrPW01QCCqcoO= NEX4XGFKdmirYnn0bY9vKG:oIIPtc{1u\WSrYYTl[EBp\WSpZXjv[{B{cWewYXzpcocheGG2aIfhfUBqdiCvb4Xz[UBPUUh|VEOgZ4VtdHNiZYjwdoV{e2mwZzDHVmUuVHWlIILldI9zfGW{IHflcoUh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBUSUdvaX7keYNm\CCJUlWtUJVkKHKncH;yeIVzKGGldHn2bZR6KGGodHXyJFQ5KGi{czDifUBtfW2rbnXzZ4Vv[2ViYYPzZZktKEmFNUCgQUAxNjByNjFOwG0v MoLzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OUm0PFMoRjJ7NEm5OFg{RC:jPh?=
Shh-light2 MWPGeY5kfGmxbjDhd5NigQ>? M4O3U2lvcGmkaYTpc44hd2ZiU33vMY1m\GmjdHXkJGhpKHOrZ37hcIlv\yCrbjDoeY1idiCVaHitcIlocHR{IHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvODB5IN88UU4> NYHJWY9FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlg2PTFpPkKyNlY5PTVzPD;hQi=>
HEK293 MlX5SpVv[3Srb36gZZN{[Xl? NYHybGpqOiCqcoO= MYLEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NWyjYnXscIVlKGO7Y3zvdIFucW6nIH\yc40hcHWvYX6gV41wKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNkBpenNiYomg[ox2d3Knc3PlcoNmKG2rY4Lvd4NweHluIFnDOVAhRSByLkCwO{DPxE1w NV[3OnFQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlg2PTFpPkKyNlY5PTVzPD;hQi=>
NIH3T3 MUTGeY5kfGmxbjDhd5NigQ>? MVvJcohq[mm2aX;uJI9nKGinZHflbI9oKHKnY3XweI9zKHOrZ37hcIlv\yCyYYToe4F6KGmwIH3veZNmKE6LSEPUN{Bk\WyuczD0doFve2[nY4Tl[EB4cXSqIFfsbU1z\XCxcoTldkBo\W6nIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMEC3NVch|ryPLh?= NXS4No1XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NlM4PjVpPkK0PVI{PzZ3PD;hQi=>
NIH/3T3 M3jOTWZ2dmO2aX;uJIF{e2G7 NFi5TnI1QCCqcoO= NULQZYw2UW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBUd26rYzDo[YRo\WixZz3pcoR2[2WmIFfsbUBtfWOrZnXyZZNmKGGldHn2bZR6KGGodHXyJFQ5KGi{czDifUBFfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvODB5MUeg{txONg>? NHPRPIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK0PVQ6PCd-M{CyOFk1QTR:L3G+
NIH3T3 MVjGeY5kfGmxbjDhd5NigQ>? MnvVOFghcHK| MVLJcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIxqdmdiaX6gcY92e2ViTlnIN3Q{KGOnbHzzJJN1[WKueTD0doFve2[nY4Tl[EB4cXSqIFfsbU1tfWOrZnXyZZNmKGOxboP0dpVkfCCrbnP1ZoF1\WRiZn;yJFQ5KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvODB5MjFOwG0v M4PafVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OEK3NVM3Lz5{NkiyO|E{PjxxYU6=
NIH3T3 MYrGeY5kfGmxbjDhd5NigQ>? MnP2OFghcHK| MVHJcohq[mm2aX;uJI9nKFOxbnnjMYlv\HWlZXSgbIVl\2Wqb3egd4lodmGubHnu[{BqdiCvb4Xz[UBPUUh|VEOgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KEeuaT3seYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7LDDJR|UxKD1iMD6wNFczKM7:TT6= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjh{MEW1OEc,OjZ6MkC1OVQ9N2F-
NIH3T3 M{KzZmZ2dmO2aX;uJIF{e2G7 MV20PEBpenN? M4C5TmlvcGmkaYTpc44hd2ZiU1jIJJNq\26jbHnu[{Bx[XSqd3H5JIlvKG2xdYPlJG5KUDOWMzDj[YxteyCvZXHzeZJm\CCjZoTldkA1QCCqcoOgZpkhT2yrLXz1Z4ln\XKjc3WgdoVxd3K2ZYKgZZN{[XluIFnDOVAhRSByLkCwO|Ih|ryPLh?= MmLiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{[zPVYoRjJ2MUe2N|k3RC:jPh?=
NIH/3T3 M4LnXWZ2dmO2aX;uJIF{e2G7 MWm0PEBpenN? M3fCc2lvcGmkaYTpc44hd2ZiaHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gcY92e2ViTlnIM|NVOyClZXzsd{Bi\nSncjC0PEBpenNiYomgS4xqNWy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuNFA4OiEQvF2u MoqwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2OE[yNFMoRjJ2NEi2NlA{RC:jPh?=
NIH/3T3 NX32eYdpTnWwY4Tpc44h[XO|YYm= M4DoXWlvcGmkaYTpc44hd2ZiaHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gcY92e2ViTlnIM|NVOyClZXzsd{BjgSCJbHmxMYx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMEC3NkDPxE1w MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDRyNUewOEc,OjR2MEW3NFQ9N2F-
NIH/3T3 NXHEbZR6TnWwY4Tpc44h[XO|YYm= NV7KT3BDUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGOjcoL5bY5oKHO2YXLsfUB1emGwc3\lZ5Rm\CCJbHmtdoVxd3K2ZYKgZ49ve3S{dXP0JIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiYYPzZZktKEmFNUCgQUAxNjByN{Kg{txONg>? MnvUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4NEKxNFEoRjJ6NkSyNVAyRC:jPh?=
C3H10T1/2 MYXGeY5kfGmxbjDhd5NigQ>? NF\VbGM3KGi{cx?= NV2xUFkyUW6qaXLpeIlwdiCxZjDTRWcucW6mdXPl[EBlcW[oZYLlcpRq[XSrb36gc4YhdW:3c3WgcYV{\W6laIntZYwheGy3cnnwc5RmdnRiQ{PINVBVOS9{IHPlcIx{KHSxIHHsb4FtcW6nIIDoc5NxcGG2YYPlJJBwe2m2aY\lJI9me2WxYnzhd5R{KGGodHXyJFYhcHK|LDDJR|UxKD1iMD6wNVEh|ryPLh?= MnrNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{Nki1OVEoRjJ{Mk[4OVUyRC:jPh?=
HCC827 M33tOGZ2dmO2aX;uJIF{e2G7 MVPEbZNxdGGlZX3lcpQhd2ZiW{PIYU1kgWOub4DhcYlv\SCocn;tJHNOVyCYNEC0UUBufXSjboSgbY4h\2WoaYTpcoljKHKnc3nzeIFvfCCqdX3hckBJS0N6MkegZ4VtdHNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiS3mgQUAxNjBzMkKg{txONg>? MlPGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OEexOVYoRjJ6N{i3NVU3RC:jPh?=
C3H10T1/2 MWTGeY5kfGmxbjDhd5NigQ>? MkTrTY5pcWKrdHnvckBw\iCVSFigbY4hdW:3c3WgR|NJOTCWMT:yJINmdGy|IHL5JGdtcS2udXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTBiPTCwMlAyOyEQvF2u NIfpVms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUexOlI6Pid-MUm3NVYzQTZ:L3G+
S12 MnjoSpVv[3Srb36gZZN{[Xl? MVvJcohq[mm2aX;uJI9nKEinZHflbI9oKHOrZ37hcIlv\yCrbjDtc5V{\SCVMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKHKnY3XweI9zNW2nZHnheIVlKEeuaUGgcXJPSSCneIDy[ZN{cW:wIHL5JJJmeG:{dHXyJIdmdmViYYPzZZktKEmQSDC9JFAvODF|IN88UU4> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh5NUe0NUc,OjB6N{W3OFE9N2F-
TM3 MXnGeY5kfGmxbjDhd5NigQ>? MmDwOFghcHK| MofRTY5pcWKrdHnvckBw\iCKaDDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDUUVMh[2WubIOgZZN{\XO|ZXSgZZMh\G:5boLl[5Vt[XSrb36gc4YhT2yrMTDn[Y5mKGW6cILld5Nqd25iYX\0[ZIhPDhiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygSWM2OCB;IECuNFE{KM7:TT6= NWP3bZQ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5O|YzOTVpPkK2PVc3OjF3PD;hQi=>
NIH/3T3 MnG4SpVv[3Srb36gZZN{[Xl? MmOzTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCQSVivN3Q{KGOnbHzzJIV5eHKnc4Ppcochf2muZDD0fZBmKFOvbzDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iR3zpJI1TVkFiZYjwdoV{e2mxbjDifUBTXC2SQ2KgcYV1cG:mLDDJR|UxKD1iMD6wNVQ1KM7:TT6= NGfDeoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{ixNFU6OSd-Mke4NVA2QTF:L3G+
S12 MUnGeY5kfGmxbjDhd5NigQ>? NYrjenlrPDhiaILz M1j3NWlvcGmkaYTpc44hd2ZiaIXtZY4hW0iKIIDheIh4[XliaX6gcY92e2ViU{GyJINmdGy|IHHzd4V{e2WmIHHzJGdNUS2vZXTpZZRm\CC2cnHud4NzcXC2aX;uZYwh[WO2aY\peJkh[W[2ZYKgOFghcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMEG1JO69VS5? M3XNO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNEO4OVI4Lz5{MUSzPFUzPzxxYU6=
NIH/3T3 NYLZNXVITnWwY4Tpc44h[XO|YYm= NU\jN3hpUW6qaXLpeIlwdiCxZjDTUW8hcW5ibX;1d4UhVkmKL{PUN{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGPBS{1qdmS3Y3XkJIhm\GenaH;nMY1m\GmjdHXkJIx2dWmwZYPj[Y5k\SC|aXfuZYxqdmdiYomgS3JGNWy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuNFE3KM7:TT6= MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjFzOUWwNEc,OjZzMUm1NFA9N2F-
U2OS NWqzR41LTnWwY4Tpc44h[XO|YYm= NEnCVVkzKGi{cx?= MYfEbZNxdGGlZX3lcpQhd2ZiW{PIYYN6[2yxcHHtbY5mKG[{b32ge4lt\CC2eYDlJHNudyCneIDy[ZN{\WRiaX6gWVJQWyClZXzsd{Bi\nSncjCyJIhzeyCkeTDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCNaTC9JFAvODF4MjFOwG0v MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4M{WyNkc,OjNyNkO1NlI9N2F-
NIH3T3 MlXHSpVv[3Srb36gZZN{[Xl? MlvrN|AhcHK| NX3aeWpHUW6qaXLpeIlwdiCxZjDTcY8hemWlZYD0c5IhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iTlnIN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gV41wKGGpb37pd5QhW0GJLXnu[JVk\WRiR2LFJIFkfGm4YYTpc44h[W[2ZYKgN|AhcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMEKzJO69VS5? M2LYXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{K2PFA4Lz5{NEeyOlgxPzxxYU6=
medulloblastoma cell MlnuRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M2TE[lM3KGi{cx?= NEfPZ|FCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JHB1[2htLz2gZY5lKHB3Mz2vMUBud3W|ZTDt[YR2dGyxYnzhd5RwdWFiY3XscJMh[W[2ZYKgN|YhcHK|IHL5JGJz\HViaX7jc5Jxd3KjdHnvckBie3OjeTygTWM2OCB;IECuNFMxPCEQvF2u NIrPeYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[4PFI4QCd-Mki2PFgzPzh:L3G+
Shh Light2 M2DsbGZ2dmO2aX;uJIF{e2G7 NH\rcldCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IIPtc491cGWwZXSgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hdW:3c3WgV4hpKEyrZ3j0NkBk\WyuczDjc{1mgHC{ZYPzbY5oKEeuaT3k[ZBmdmSnboSgdoVxd3K2ZYKg[4Vv\SCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEiqIIPp[45idGmwZzDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvODN|IN88UU4> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR7MUS1PUc,OjR2OUG0OVk9N2F-
Light2 MkfSSpVv[3Srb36gZZN{[Xl? MnruTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCOaXfoeFIh[2WubIOgbY4hW2iqIHPvcoRqfGmxbnXkJI1m\Gm3bTDifUBIdGlvbIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyIE2gNE4xOzlizszNMi=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh5M{OwN{c,Ojh6N{OzNFM9N2F-
Shh Light2 NWHRNphjTnWwY4Tpc44h[XO|YYm= NHPqcnJKdmirYnn0bY9vKG:oIGPtc{1u\WSrYYTl[EBJcCC|aXfuZYxtcW6pIIDheIh4[XliaX6gcY92e2ViU3joJGxq\2i2MjDj[YxteyCkeTDHcIkudHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxKD1iMD6wN|kzKM7:TT6= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd|NkC2N{c,Ojd5M{[wOlM9N2F-
ASZ001 NWnpTGxbTnWwY4Tpc44h[XO|YYm= NHrhXZA1QCCqcoO= Mlu5TY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC|aXfuZYxqdmdiaX6gcY92e2ViQWPaNFAyKGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaUGgcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDR6IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjB2IN88UU4> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEexOkc,OjR7MEC3NVY9N2F-
NIH/3T3 NX\EeJFITnWwY4Tpc44h[XO|YYm= M1zPOVQ5KGi{cx?= Mnu1RY51[WexbnnzeEBi[3Srdnn0fUBifCCVbX:gdoVk\XC2b4KgbY4hdW:3c3WgUmlJNzOWMzDj[YxteyCqYYLic5JqdmdiR2LFMWx2[yCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKFODRz3pcoR2[2WmIFjoJJNq\26jbHnu[{Bx[XSqd3H5JJBz\WmwY4XiZZRm\CC5aYToJINmdGy|IH\vcIxwf2WmIHL5JHNCTyCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkA1QCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxKD1iMD6wOFYh|ryPLh?= MoS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl6NUeyO|UoRjJ7OEW3Nlc2RC:jPh?=
Light2 Mk[0SpVv[3Srb36gZZN{[Xl? M2TXW|MxKGi{cx?= MmnwTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCOaXfoeFIh[2WubIOgbY4hW2iqIHPvcoRqfGmxbnXkJI1m\Gm3bTDh[pRmeiB|MDDodpMh[nliR3zpMXJmdmmubHGgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC5yNTFOwG0v MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODB7OUK1O{c,OzByOUmyOVc9N2F-
C3H10T1/2 NGXqOlRHfW6ldHnvckBie3OjeR?= NH\Ne3M3KGSjeYO= NVLhO|NkUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeT3t[YRq[XSnZDDkbYZn\XKnboTpZZRqd25ib3[gcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJHNCTy2rbnT1Z4VlKEGOUDDhZ5Rqfmm2eTDh[pRmeiB4IHThfZMh[nliY3jlcYltfW2rbnXzZ4Vv[2VvYnHz[YQh[XO|YYmsJGlEPTBiPTCwMlA2KM7:TT6= M12yTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMEm5NlU4Lz5|MEC5PVI2PzxxYU6=
DaOY M2PEOmZ2dmO2aX;uJIF{e2G7 NV\DZVFxPDhiaILz NHzJeZhKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBpfW2jbjDEZW9[KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaUGgcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDR6IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjB6NjFOwG0v NXXEdlBDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4OTZpPkK0PVAxPzF4PD;hQi=>
C3H10T1/2 MkTlSpVv[3Srb36gZZN{[Xl? MUCxJIhz M3SxSWlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBxfXKvb4LwbIFucW6nLXnu[JVk\WRiaX7jdoVie2ViaX6gZYxs[WyrbnWgdIhwe3CqYYThd4UhdGW4ZXygeZNqdmdiQ1TQMZN1[XJiYYOgd5Vje3S{YYTlJJBz\XS{ZXH0[YQh\m:{IEGgbJIh\m:ubH;3[YQh[nlicIXycY9zeGijbXnu[UBi\GSrdHnvckBu\WG|dYLl[EBi\nSncjC1JIRigSxiSVO1NEA:KDBwMUSg{txONg>? MorBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NEe5N|koRjJ6OUS3PVM6RC:jPh?=
M210B4 MkPzSpVv[3Srb36gZZN{[Xl? NH[4dHozPCCqcoO= Mne5TY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC|aXfuZYxqdmdiaX6gcY92e2ViTUKxNGI1KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKFC2Y3igcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjF2IN88UU4> M{LOU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwO|E3Lz5{NEmwNFcyPjxxYU6=
M210B4 MXPGeY5kfGmxbjDhd5NigQ>? NWKxPYNVOjRiaILz NF\3NJdKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBud3W|ZTDNNlExSjRiY3XscJMh[XO|ZYPz[YQh[XNiZH;3cpJm\3WuYYTpc44hd2ZiR3zpNUBuWk6DIHX4dJJme3Orb36gZYZ1\XJiMkSgbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyxiSVO1NEA:KDBwMjFOwG0v MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEexOkc,OjR7MEC3NVY9N2F-
Shh Light2 MnjvSpVv[3Srb36gZZN{[Xl? MkmyNUBpeg>? NIXMVopKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBud3W|ZTDTbIghVGmpaISyJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCydYLtc5JxcGGvaX7lMYlv\HWlZXSgS4xqKG2nZHnheIVlKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBxemW2cnXheIVlKG[xcjCxJIhzKG[xbHzve4VlKGK7IID1do1wenCqYX3pcoUh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgOFghcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwOUig{txONg>? MnHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7NEe5N|koRjJ6OUS3PVM6RC:jPh?=
NIH/3T3 NUTTW2tITnWwY4Tpc44h[XO|YYm= NHTsOXpKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIF7JTE8{XDNiY3XscJMhcGG{Yn;ybY5oKFOvbzDEOFc4UCCvdYThcpQh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGdtcSCvUl7BJIV5eHKnc4Ppc44h[nliUmStVGNTKG2ndHjv[EwhUUN3MDC9JFEvOTl4IN88UU4> M2jZdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OEGwOVkyLz5{N{ixNFU6OTxxYU6=
S12 Ml35SpVv[3Srb36gZZN{[Xl? NYfqSG9pPDhiaILz MnXiTY5pcWKrdHnvckBw\iCqdX3hckBUUEhicHH0bJdigSCrbjDtc5V{\SCVMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgS2xKNW2nZHnheIVlKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFAvPSCvZz;tUEBpfW2jbjDhcJBp[S1zLXHjbYQh\2y7Y3;wdo91\WmwLDDJR|UxKD1iMT63OUDPxE1w NH7jWpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUSzPFUzPyd-MkG0N|g2Ojd:L3G+
Calu6 NWn0WGM6TnWwY4Tpc44h[XO|YYm= M4XY[VQhcHK| NFvlbWJRdGG|bXGgZ49v[2WwdILheIlwdiCrbjDoeY1idiCFYXz1OkBk\WyuczD4[Y5w\3KjZoTl[EBqdiCwdXTlJI1wfXOnIHH0JFc2KG2pL3vnMEBxdyCkaXSgcYVie3W{ZXSgZYZ1\XJiNDDodpMheG:|dDDmbYZ1cCCmb4PlMEBEeCB;IEKzJO69VS5? NIjKZnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUexOlI6Pid-MUm3NVYzQTZ:L3G+
Calu-6 NFrlUHVHfW6ldHnvckBie3OjeR?= MUPQcIF{dWFiY3;uZ4VvfHKjdHnvckBqdiCFYXz1MVYh[2WubIOgfIVvd2e{YX\0[YQhdnWmZTDtc5V{\SCSSz;QSEBud2SnbDDheEA4PSCvZz;r[{wheG9iYnnkJIZweiB3IHThfZMhdWWjc4Xy[YQhPCCqcoOgdI9{fCCuYYP0JIRwe2VicnXsZZRqfmVidH:geY51emWjdHXkJINwdnS{b3ysJGNxKD1iMkOg{txONg>? NG\xW489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi3OVc1OSd-MkC4O|U4PDF:L3G+
T47D MYXDfZRwfG:6aXPpeJkh[XO|YYm= MV:3NkBpenN? MoHnR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWFQ4TCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gOFEvOzRizszNMi=> NEezepA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUGwO|QzQSd-MkmxNFc1Ojl:L3G+
LS180 NGjVZYZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M{jNVVczKGi{cx?= NXzPUm9SSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMV|E5OCClZXzsd{Bi\nSncjC3NkBpenNiYomgd5Vt\m:{aH;kZY1qdmVvQjDhd5NigSxiSVO1NEA:KDR3IN88UU4> Mn;BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByOUmyOVcoRjNyMEm5NlU4RC:jPh?=
LS174T M3nLPWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M4LrN|czKGi{cx?= MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEyVMUe0WEBk\WyuczDh[pRmeiB5MjDodpMh[nliQ3XscHRqfGW{LVfsc{BtfW2rbnXzZ4VvfCClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OCB;IES1MlgyKM7:TT6= MnfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ6MkC1OVQoRjJ4OEKwOVU1RC:jPh?=
BxPC3 M4\RPWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MUO3NkBpenN? NWOzZ412SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDCfHBEOyClZXzsd{Bi\nSncjC3NkBpenNiYomgR4VtdFSrdHXyMWdtdyCudX3pcoV{[2WwdDDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFQ4Njl3IN88UU4> M3i3NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OEKwOVU1Lz5{NkiyNFU2PDxxYU6=
medulloblastoma cell NEX1VnpCdnSrdIXtc5Ih[XO|YYm= MUTBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCvZXT1cIxw[myjc4TvcYEh[2WubIOgfIVvd2e{YX\0[YQhcW5iUITjbEAsNy1ibX;1d4Uh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6geJVud3Jidn;seY1mKGG2IEGyMlUhdWdxa3esJJBwKGKrZD6= M1HG[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{G2Nlk3Lz5zOUexOlI6PjxxYU6=
S12 NGj5TmtHfW6ldHnvckBie3OjeR?= MXG0PEBpenN? MmXDTY5pcWKrdHnvckBw\iCqdX3hckBUUEhicHH0bJdigSCrbjDtc5V{\SCVMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgS2xKNW2nZHnheIVlKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFEhdWdxbVygbJVu[W5iYXzwbIEuOS2jY3nkJIdtgWOxcILveIVqdi5? NI\YPYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUSzPFUzPyd-MkG0N|g2Ojd:L3G+
Shh Light2 NXjKe2lETnWwY4Tpc44h[XO|YYm= MlrENE4yKHWP MVuzOkBpenN? NFjNdG9KdmirYnn0bY9vKG:oIGPtc{1u\WSrYYTl[EBJcCC|aXfuZYxtcW6pIIDheIh4[XliaX6gcY92e2ViU3joJGxq\2i2MjDj[YxteyCjc4Pld5Nm\CCjczDzeZBxemW|c3nvckBw\iCJbHmxMY1TVkFiZYjwdoV{e2mxbjDheEAxNjFidV2gcYVie3W{ZXSgZYZ1\XJiM{[gbJJ{KGK7IGLUMZFRS1JiYX7hcJl{cXNw NYnpeVFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3N|YxPjNpPkK3O|M3ODZ|PD;hQi=>
SW1353 NV3peWFVSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MmPRNVAhfU1? MXG0PEBpenN? NET5[YFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPXNVM2OyClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCjdDCxNEB2VSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6Ng>? MoruQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2OUG0OVkoRjJ2NEmxOFU6RC:jPh?=
DaOY M1OzfmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NIXiNZUyOCC3TR?= NYPP[Iw3PDhiaILz M3nndWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRHHPXUBk\WyuczDhd5Nme3OnZDDhd{Boem:5dHigbY5pcWKrdHnvckBifCBzMDD1UUBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5Mi=> M1jPbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEmxOFU6Lz5{NES5NVQ2QTxxYU6=
A549 MnjJRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NHPFRXIyOCC3TR?= NVHDOYZlPDhiaILz NF\yS2lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[XRiMUCgeW0h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfU4> Mmr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2OUG0OVkoRjJ2NEmxOFU6RC:jPh?=
HCT116 M3nVOWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M2XmV|ExKHWP MVW0PEBpenN? M{jhUmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vKGG2IEGwJJVOKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZkv M1\D[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEmxOFU6Lz5{NES5NVQ2QTxxYU6=
NIH/3T3 MmjLSpVv[3Srb36gZZN{[Xl? NHz4SIQyOCC3TR?= M4LyW2lvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIHnuJI1wfXOnIF7JTE8{XDNiY3XscJMh[XO|ZYPz[YQh[XNiZH;3ckBz\We3bHH0bY9vKG:oIH3QWGNJOSCvUl7BJIV5eHKnc4Ppc44h[XRiMUCgeW0v NHXGOYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES5NVQ2QSd-MkS0PVE1PTl:L3G+
MSC Mnm1SpVv[3Srb36gZZN{[Xl? NGPhfo4zOCC2bzC4NEB2VQ>? NHHnendKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDpckBud3W|ZTDNV2Mh[2WubIOgZZN{\XO|ZXSgZZMh\G:5bjDy[Yd2dGG2aX;uJI9nKGGua3HsbY5mKHCqb4PwbIF1[XOnIHH0JFIxKHSxIEiwJJVONg>? NGD3cJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES5NVQ2QSd-MkS0PVE1PTl:L3G+
SK-N-MC NWDDU4JSeUiWUzDhd5NigQ>? NUnrTHB2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJMv M2n0RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32 NEnTc4hyUFSVIHHzd4F6 MoLBdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsd{4> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NIH3T3-Luc NXvmbJdqTnWwY4Tpc44h[XO|YYm= MWKxJJVO MmPSOFghcHK| M3\aOmlvcGmkaYTpc44hd2Zic33vMY1m\GmjdHXkJIhm\GenaH;nJJNq\26jbHnu[{Bx[XSqd3H5JIlvKG2xdYPlJG5KUDOWMz3MeYMh[2WubIOgZZN{\XO|ZXSgZZMh\G:5boLl[5Vt[XSrb36gc4YhT2yrIH3SUmEh\XiycnXzd4lwdiCjdDCxJJVOKGGodHXyJFQ5KGi{czDifUBz\WGuLYTpcYUhWEOUIHHuZYx6e2m|Lh?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR7OUS4N{c,Ojl2OUm0PFM9N2F-
C3H10T1/2 MUPGeY5kfGmxbjDhd5NigQ>? NEjkNpEzPCCqcoO= MVXJcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6NW2nZHnheIVlKEeOSUGgcXJPSSCneIDy[ZN{cW:wIHnuJI1wfXOnIFOzTFExXDFxMjDj[YxteyCjZoTldkAzPCCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nzJIlvKHC{ZYPlcoNmKG:oIDixVkw{[VJuNGOsO4FTMS15YT3t[ZRpgWxvMT2oLHIqNTZvbXX0bJltcGWydHHuMVIugWxrb3P0ZYh6\HKxLUHIMYlv\GWwLUStfYwhOy2qeXTyc5h6[mWwen;heIUv NUW2ZmxZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3N|A6QDRpPkK0O|MxQTh2PD;hQi=>
C3H10T1/2 MWDGeY5kfGmxbjDhd5NigQ>? MUKyOEBpenN? NVL3fmN7UW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeT3t[YRq[XSnZDDHUGkyKG2UTlGg[ZhxemW|c3nvckBqdiCvb4Xz[UBEO0hzMGSxM|Ih[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IGLUMXBEWiCjbnHsfZNqeyCrbjDwdoV{\W6lZTDv[kBXTDNw MlzkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5M{C5PFQoRjJ2N{OwPVg1RC:jPh?=
NIH/3T3 MWXGeY5kfGmxbjDhd5NigQ>? NUflR2dnOTByIH7N M13WT|M3KGi{cx?= M{LSNGlvcGmkaYTpc44hd2ZiaHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gcY92e2ViTlnIM|NVOyClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCVaHitbY5lfWOnZDDHcIkyKG2UTlGg[ZhxemW|c3nvckBt\X[nbIOgZZQhOTByIH7NJIFnfGW{IEO2JIhzeyCkeTDSWE1yWEOUIHHuZYx6e2m|Lh?= NVPxW2dURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4O|M{ODNpPkK4PFc{OzB|PD;hQi=>
C3H10T1/2 MoLzSpVv[3Srb36gZZN{[Xl? M2X6R|ExOCCwTR?= Mlj3N|YhcHK| NFK4NGdKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIFOzTFExXDFxMjDj[YxteyCjc4Pld5Nm\CCjczDkc5dvemWpdXzheIlwdiCxZjDTbIgucW6mdXPl[EBIdGlzIH3SUmEh\XiycnXzd4lwdiCuZY\lcJMh[XRiMUCwJI5OKGGodHXyJFM3KGi{czDifUBTXC2zUFPSJIFv[Wy7c3nzMi=> M2PweVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEezN|A{Lz5{OEi3N|MxOzxxYU6=
NIH/3T3 NHf0TJlHfW6ldHnvckBie3OjeR?= NYLHTnBCOTByIH7N MUizOkBpenN? NUfTfpJPUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGG|c3Xzd4VlKGG|IHTve45z\We3bHH0bY9vKG:oIGPobE1qdmS3Y3XkJJB1[2hzIH3SUmEh\XiycnXzd4lwdiCuZY\lcJMh[XRiMUCwJI5OKGGodHXyJFM3KGi{czDifUBTXC2zUFPSJIFv[Wy7c3nzMi=> M{nrPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEezN|A{Lz5{OEi3N|MxOzxxYU6=
C3H10T1/2 M1;xZ2Z2dmO2aX;uJIF{e2G7 MVWxNFAhdk1? M{TtSVM3KGi{cx?= MUnJcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6KGmwIH3veZNmKEN|SEGwWFEwOiClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCVaHitbY5lfWOnZDDweINpOSCvUl7BJIV5eHKnc4Ppc44hdGW4ZXzzJIF1KDFyMDDuUUBi\nSncjCzOkBpenNiYomgVnQueVCFUjDhcoFtgXOrcz6= NU\rUo52RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4O|M{ODNpPkK4PFc{OzB|PD;hQi=>
medulloblastoma cell MmXVRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MoG2eZAhfG9iM{CgeW0> NXLITpR5PzJiaILz NETLS3BCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIGD0Z4gsNy1icEWzMU8uKG2nZIXscI9jdGG|dH;tZUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEBxem:uaX\ldoF1cW:wIIXwJJRwKDNyIIXNJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmu NWfh[YNQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4O|M{ODNpPkK4PFc{OzB|PD;hQi=>
medulloblastoma cell MX;BdI9xfG:|aYOgZZN{[Xl? MX\JcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIH3veZNmKFC2Y3irM{0heDV|LT:tJI1m\HWubH;icIF{fG:vYTDj[YxteyCrbYDsZY51\WRiaX6gZZRpgW2rYzDueYRmKG2xdYPlJIF1KDF{LkWgcYcwc2duIHnwJIJq\CCob4KgNVUh[2:wc3XjeZRqfmViZHH5d{BjgSCWVV7FUEBie3OjeT6= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh5M{OwN{c,Ojh6N{OzNFM9N2F-
NIH/3T3 MUDGeY5kfGmxbjDhd5NigQ>? NIHj[phKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHX4dJJme3OnZDDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcGWmZ3Xoc4cucW6mdXPl[EBIdGlvMjDhZ4N2dXWuYYTpc44h[XRidHnwJI9nKHC{aX3hdpkh[2muaXGgZpkhTEGSSTDzeIFqdmmwZzDiZZNm\CClb37mc4NidCCvaXPyc5Nkd3CrYzDhcoFtgXOrcz6= NGrIcG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{ixNFU6OSd-Mke4NVA2QTF:L3G+
NIH/3T3 MXTGeY5kfGmxbjDhd5NigQ>? NHrqPXFKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIF7JTE8{XDNiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBp\WSpZXjv[{1qdmS3Y3XkJHNudy2HR1\QJINqdGmjcomgeJJidnOub3PheIlwdiCkeTDERXBKKHO2YXnubY5oKGKjc3XkJINwdm[xY3HsJI1q[3Kxc3PvdIlkKGGwYXz5d4l{Ng>? M4ThRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OEGwOVkyLz5{N{ixNFU6OTxxYU6=
medulloblastoma cell NFPwbVFCdnSrLYT1cY9zKGG|c3H5 NV;qd5FTSW62aT30eY1weiCjY4Tpeol1gSCjZ3HpcpN1KG2xdYPlJHB1[2htLz2gdFU{NS9vIH3l[JVtdG:kbHHzeI9u[SClZXzsd{BqdXCuYX70[YQhcW5iYYTofY1q[yCwdXTlJI1wfXOnIHHzd4V{e2WmIHHzJJR2dW:{IHfyc5d1cCCrbnjpZol1cW:wIHH0JFIxKG2pL3vnMEBxdyCjZH3pcol{fGW{ZXSgeJdq[2ViZHHpcJkhfmmjIHfheoFo\SCvZXHzeZJm\CCndnXyfUBwfGincjDkZZkh\HW{aX7nJINwdXCxdX7kJIRwe2mwZz6= NIixXZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUezPVcyPCd-Mkm3N|k4OTR:L3G+
C3H10T1/2 M1W4RmZ2dmO2aX;uJIF{e2G7 MWG0MlEhfG9iMUCwNEBvVQ>? NV7pZWFRSWyub4P0[ZJq[yCrbnjpZol1cW:wIH;mJHNudyCrbjDtc5V{\SCFM1ixNHQyNzJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBxfXKvb4LwbIFucW6nLXnu[JVk\WRiR3zpNUB1emGwc3PybZB1cW:wYXygZYN1cX[rdImgZZQhPC5zIITvJFExODBibl2gZpkheVCFcjDhcoFtgXOrcz6= M3e1OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEe0OVQyLz5{M{C3OFU1OTxxYU6=
C3H10T1/2 M4HseWZ2dmO2aX;uJIF{e2G7 MkHqOE4yKHSxIEGwNFAhdk1? NF7jbWtEd22yZYTpeIl3\SCrbnjpZol1cW:wIH;mJHNudyCrbjDtc5V{\SCFM1ixNHQyNzJiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBUSUdvaX7keYNm\CCJbHmxJJRz[W6|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDheEA1NjFidH:gNVAxOCCwTTDifUByWEOUIHHuZYx6e2m|Lh?= NFLPeFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C3OFU1OSd-MkOwO|Q2PDF:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Bcl-2 / Bax; 

PubMed: 28195165     


GDC-0449 treatment affected the expression levels of cell cycle-related protein levels. U251 cells were treated with 0.1% DMSO or GDC-0449 at the indicated concentrations for 24 h. Cells were then harvested and examined using Western blot analysis with the indicated antibodies.

p53 / Cyclin D1 / p21; 

PubMed: 28195165     


GDC-0449 treatment affected the expression levels of cell cycle-related protein levels. U251 cells were treated with 0.1% DMSO or GDC-0449 at the indicated concentrations for 24 h. Cells were then harvested and examined using Western blot analysis with the indicated antibodies.

Gli1 / SOX2 / OCT4; 

PubMed: 26418365     


The activation of Hedgehog pathways and CSC markers were tested by immunoblotting analysis in Hs578T and BT549 cells. β‐Actin was used as a loading control.

p-GSK3β / GSK3β / p-Akt / Akt / Gli1; 

PubMed: 27143997     


Cells were pretreated with or without 30 μM GDC-0449 for 2 h before TMT treatment. After incubation with 3.75 μM TMT for another 24 h, the expression levels of proteins were determined through Western blot analysis.

Fas / DR4 / DR5 / Cleaved PARP / Bcl-2 / Cleaved caspase-3 / PDGFRα; 

PubMed: 22087285     


Pancreatic CSCs were treated with GDC-0449 (0, 1, 5 and 10 µM) for 48 h. The expression of Fas, DR4/TRAIL-R1, DR5/TRAIL-R2, PARP cleavage, Bcl-2, and Caspase-3 by the Western blot analysis. β-Actin was used as a loading control.

28195165 26418365 27143997 22087285
Immunofluorescence
Gli1 / Gli2; 

PubMed: 22087285     


GDC-0449 inhibits expression of Gli1 and Gli2 in human pancreatic CSCs. The cells were seeded on fibronectin-coated coverslips and treated with GDC-0449 (10 µM) for 48 h. Subsequently, cells were fixed with 4% paraformaldehyde, blocked in 10% normal goat serum and stained with Gli1 and Gli2 primary antibodies (1∶100) for 16 h at 4°C and washed with PBS. Afterwards, cells were incubated with fluorescently labeled secondary antibody (1∶200) along with DAPI (1 mg/ml) for 1 h at room temperature and cells were mounted and visualized under a fluorescent microscope. For better visuality, the color of DAPI was changed from blue to red.

22087285
Growth inhibition assay
Cell viability; 

PubMed: 29042665     


GDC-0449 improves the anti-proliferation activity in NIH-3T3 cells but not in BxPC-3, Panc-1, MIAPaca-2 and SW1990 cells when cultured alone. The cytotoxicities of GDC-0449, Dox or Dox with GDC-0449 (5 μM or 10 μM) were measured by performing a 48-h MTT assay in BxPC-3 (A), Panc-1 (B), MIAPaca-2 (C), SW1990 (D) and NIH-3T3 (E) cells. Apoptosis rate was detected by flow cytometry (Annexin V staining) analysis after treatment with Dox (200 nM) with or without GDC-0449 for 24 h (F). Data are presented as the mean ± SD. *P < 0.05, **P < 0.01.

29042665
体内研究 GDC-0449已经用于治疗动物模型的成髓细胞瘤。[2] GDC-0449 抑制原代胰腺移植瘤生长,但是不抑制细胞增殖。GDC-0449按25 mg/kg 以上剂量口服给药髓母细胞瘤Ptch(+/-)异体移植物模型,引起细胞衰退,按92 mg/kg剂量处理两种配位体依赖的结肠直肠癌模型D5123和1040830,每天处理两次,抑制肿瘤生长。分析Hh通路活性和 PK/PD 模型显示GDC-0449抑制 Gli1,IC50值与GDC-0449作用于髓母细胞瘤和D5123模型的IC50值差不多(分别为0.165 μM ±11.5% 和0.267 μM ±4.83%)。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[2]
- 合并
  • Cell lines: MDCKII细胞
  • Concentrations: 20 μM
  • Incubation Time: 2小时
  • Method: MDCKII细胞按每孔3x105密度接种在24孔板中。培养液包含不同药物,实验组为50 µM VP,50 µM吲哚美辛,或者20 µM GDC-0449溶解在DMSO中,对照组只含有DMSO。然后加入非荧光的钙黄绿素-AM,最终浓度达到1.0 µM。然后在37oC温育2小时。细胞用含Ca2+,Mg2+的Hank's平衡盐溶液buffer冲洗2次,然后震荡1小时溶解在含0.01% Triton X-100的磷酸缓冲液中,然后放在4oC下过夜。溶解的物质然后转移到96孔板上,钙黄绿素引起的荧光信号用分光光度法定量。所有的实验操作需在暗中进行,所有的读数用平均值±SEM表示。
    (Only for Reference)
动物实验:[4]
- 合并
  • Animal Models: Ptch(+/-)异体移植物模型
  • Dosages: 25 mg/kg,92 mg/kg
  • Administration: 口服
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 84 mg/mL (199.38 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
12.5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 421.3
化学式

C19H14Cl2N2O3S

CAS号 879085-55-9
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03610022 Recruiting Drug: Treatment with vismodegib Metastatic Basal Cell Carcinoma|Locally Advanced Basal Cell Carcinoma University Hospital Bordeaux September 3 2018 Phase 4
NCT03035188 Unknown status Drug: Vismodegib Basal Cell Carcinoma SRH Wald-Klinikum Gera GmbH January 2017 Phase 2
NCT02781389 Completed -- Basal Cell Carcinoma University Hospital Essen|OnkoDataMed GmbH April 29 2016 --
NCT02593760 Completed Other: Placebo|Drug: Ruxolitinib|Drug: Vismodegib Myelofibrosis Hoffmann-La Roche January 25 2016 Phase 1
NCT02648048 Completed Drug: Pirfenidone|Drug: Vismodegib Idiopathic Pulmonary Fibrosis Hoffmann-La Roche January 15 2016 Phase 1
NCT02436408 Completed Drug: Vismodegib Carcinoma Basal Cell University of Michigan Rogel Cancer Center July 15 2015 Phase 4

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
免费分装抑制剂

Hedgehog/Smoothened Signaling Pathway Map

Hedgehog/Smoothened Inhibitors with Unique Features

相关Hedgehog/Smoothened产品

Tags: 购买Vismodegib (GDC-0449) | Vismodegib (GDC-0449)供应商 | 采购Vismodegib (GDC-0449) | Vismodegib (GDC-0449)价格 | Vismodegib (GDC-0449)生产 | 订购Vismodegib (GDC-0449) | Vismodegib (GDC-0449)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID