Vismodegib (GDC-0449)

For research use only. Not for use in humans.

目录号:S1082 中文名称:维莫德吉

Vismodegib (GDC-0449) Chemical Structure

CAS No. 879085-55-9

Vismodegib (GDC-0449)是一种新型有效的,特异性hedgehog抑制剂,无细胞试验中IC50为3 nM,也会抑制P-gp,IC50为3.0μM。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1179.52 现货
RMB 730.63 现货
RMB 3046.91 现货
RMB 8763.3 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Vismodegib (GDC-0449)发表文献144篇:

产品安全说明书

Hedgehog/Smoothened抑制剂选择性比较

生物活性

产品描述 Vismodegib (GDC-0449)是一种新型有效的,特异性hedgehog抑制剂,无细胞试验中IC50为3 nM,也会抑制P-gp,IC50为3.0μM。
靶点
Hedgehog [1]
(Cell-free assay)
3 nM
体外研究

GDC-0449有效抑制hedgehog通路,是一种新型及特定合成的小分子抑制剂,IC50为3nM。[1] GDC-0449作用于Hedgehog信号通路,阻断Hedgehog配位体,即细胞表面受体PTCH 及SMO的活性,从而阻断Hedgehog信号通路。在组织生长和修复方面,Hedgehog信号通路意义重大。Hedgehog通路信号的异常激活,及不受控制的细胞增殖,可能与Hedgehog配位体,即细胞表面受体PTCH 及SMO的突变有关。在体外,GDC-0449在不抑制胰脏细胞增殖的前提下阻止原代胰脏移植物的生长,这项结果最近已经应用到临床。GDC-0449也抑制ABCG2, P-糖蛋白, 及和MDR有关联的重要MRP1 ABC载体。GDC-0449也阻断其他多种ABC载体。ABC载体属于一个膜蛋白家族,它的过量表达和耐药性有关,这也是治疗癌症的一个重大障碍。GDC-0449强抑制两种ABC载体,ABCG2/BCRP和ABCB1/P-糖蛋白,也温和抑制ABCC1/MRP1。在ABCG2过量表达的HEK293细胞中, GDC-0449可以提高荧光ABCG2底物BODIPY-哌唑嗪的保持力,也可用米托蒽醌再次处理这些细胞,产生抗肿瘤的ABCG2底物。实验使Madin-Darby犬的肾脏细胞中过量表达P糖蛋白或者MRP1,GDC-0449 提高了钙黄绿素-AM的保持力,然后载用秋水仙素处理这些细胞。另外,用米托蒽醌,托泊替康,SN-38处理的过量表达ABCG2的人类非小细胞肺癌细胞NCI-H460/par和NCI-H460/MX20,用GDC-0449再次处理。GDC-0449 作用于ABCG2 和 Pgp 的IC50值分别为1.4μM和 3.0 μM。[2]GDC-0449 改变细胞内Ca2+ 平衡,且作用于抗cisplatin的肺癌细胞, 抑制细胞生长。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
IGROV-1 MnHWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofhTWM2OD1yLkC3NlQ5KM7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
HCE-T M{XNNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTFwM{KyOFch|ryP MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
D-542MG NGHsZnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrDTWM2OD1zLki2O|M4KM7:TR?= NV72WolzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
23132-87 NV;lSWRsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\jTWM2OD12LkSwNVQ4KM7:TR?= NWS4fG51RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
HDLM-2 NYrF[lJpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjkOI9KSzVyPUiuNFQ4PjZizszN NHW0Tnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
ACN NFvFbmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXyzblAyUUN3ME24MlUxOTB7IN88US=> M1XmPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
HuO-3N1 MkTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{[3bmlEPTB;OT62NFExQCEQvF2= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
BHT-101 NEexPGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmD1TWM2OD1zMT6zPEDPxE1? NYnsNJhlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
KYSE-150 M3n2bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRTFzLkW4OFEh|ryP NUP4VGxjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
MC-IXC MlvvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV75RYR7UUN3ME2xNk4zOjl{IN88US=> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
D-423MG MkK3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXixdGc3UUN3ME2xNk44PjV5IN88US=> MlrSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
NY MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTF2Lki5NFMh|ryP NHrqVGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
HOS MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDMS3hKSzVyPUG1MlY4OTlizszN NUXoW4RpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
NB7 M1Sxemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\xb3ZKSzVyPUG1Mlg6OSEQvF2= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
DMS-273 M4H3b2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LUZWlEPTB;MU[uOlcyOyEQvF2= M3vuUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
MDA-MB-361 NX7IOlhXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{[1UWlEPTB;MUeuNlcyOSEQvF2= NGrWV209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
DU-145 MkLFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37KNWlEPTB;MUiuN|Ih|ryP NGrJR5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
NCI-H82 NFvDS4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTF7LkizPFYh|ryP MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
NCI-SNU-1 M{nGOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTJyLkCxPVYh|ryP MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
GCT NY\wS21TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTJyLki4NlQh|ryP M2HkUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
C2BBe1 M3\Pc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWXJR|UxRTJzLkGwOVgh|ryP NFWxNo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
LB2241-RCC MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PBOGlEPTB;MkGuPFQ1OSEQvF2= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
COLO-829 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3S3b2lEPTB;MkKuNVg4OSEQvF2= NH7DR4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
EW-11 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfaTWM2OD1{Mj64NFIzKM7:TR?= NX20WJZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
NCI-H526 NEfEcmNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoHkTWM2OD1{Mz60O|E4KM7:TR?= NIO2N2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
SF295 Ml\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUDT[XE2UUN3ME2yOE4xOjV{IN88US=> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
D-566MG NIrFT4hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUHBWmJEUUN3ME2yOU4zQTR|IN88US=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
8505C NVjWdJFMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nQe2lEPTB;MkWuOlM{OSEQvF2= M1i3eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
HT-29 NFr3dHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\KfmlEPTB;Mk[uNFQ{OSEQvF2= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
NBsusSR MmfOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3v5[2lEPTB;Mk[uPFAxPiEQvF2= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
BV-173 NH3HepVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnnUTWM2OD1{OD6zNVgzKM7:TR?= M37US|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
CTB-1 NFTrTodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXZTWM2OD1|MD6xNFMyKM7:TR?= NYiySokzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
JAR NF\jdpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIT4NJlKSzVyPUOyMlU{PzFizszN M3HYWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
CAMA-1 NYLt[o02T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknmTWM2OD1|Mz60OlE2KM7:TR?= MkLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
CAL-51 NU\oWFl4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;aRnRKSzVyPUO0MlcyPzZizszN MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
A172 NWjFVnJNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nFSWlEPTB;M{euOFkzOSEQvF2= NHvpVJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
QIMR-WIL NXrpbJk4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYTDVZFWUUN3ME2zPE4xPzB6IN88US=> MkWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
AsPC-1 NVS3c|FkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fzcWlEPTB;M{iuOFY2OSEQvF2= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
MKN7 NICydpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkLmTWM2OD1|OT6wNFc6KM7:TR?= MmS4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
ONS-76 NV;GVWlxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFux[4ZKSzVyPUSzMlMxPTdizszN NIjj[Ww9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
RS4-11 M{DEN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnuwTWM2OD12ND6wO|UzKM7:TR?= MnnGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
NOS-1 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\WWlRKSzVyPUS0MlYxOzFizszN MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
A101D MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTR2LkiwNlMh|ryP MojyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFc{PDF5Lze+V2FPT0WUPD;hQi=>
HCC1806 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PaVGlEPTB;NE[uNVE1QCEQvF2= M3XHb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
CAL-27 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTR5LkeyOFYh|ryP M4C4Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
BT-549 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGX1WpBKSzVyPUS4MlU{OTVizszN NW\jN2d7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
LCLC-97TM1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLZUnUzUUN3ME20PU4zPDF|IN88US=> NHHKSmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1PzN2MUevK|5USU6JRWK8M4E,
A4-Fuk M4fWTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXnqcoNwUUN3ME20PU45PDlizszN MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
OVCAR-4 M{HvV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkLmTWM2OD13MD6wOlAyKM7:TR?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0O|M1OTdxJ{7TRW5ITVJ:L3G+
HD-MY-Z NXXtUGlPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTVyLke3OlQh|ryP NW\iRW5GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDd|NEG3M{c,W0GQR1XSQE9iRg>?
NCI-H292 M2n6T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzndFg3UUN3ME21NE45PzV6IN88US=> M2PH[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQ4OzRzNz:nQnNCVkeHUkyvZV4>
Sk-ChA-1  NYXGVpFlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVKwMlI26oDVNUCg{txO M2nXNlczKGh? MmC1TWM2OD15ND61OOKyOi53ON88US=> NFL1O4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe0NlQ5Oid-MkW3OFI1QDJ:L3G+
Mz-ChA-1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnm1NE4zPeLCk{WwJO69VQ>? NHXISVQ4OiCq NEXEcm1KSzVyPUW0Mlk4yrF|LkS1{txO NF;uTIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe0NlQ5Oid-MkW3OFI1QDJ:L3G+
Smo-WT NVzaU|hbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX:1OXBxUUN3MNMgc4YhOTUEoH7N M3q1TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkmxNVA1Lz5{NEK5NVExPDxxYU6=
Smo-D473H  NHn0[YFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH;lN|NKSzVywrDv[kA4NjIEoN88US=> M{n6O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkmxNVA1Lz5{NEK5NVExPDxxYU6=
K562 Mkn4SpVv[3Srb36gRZN{[Xl? MWCxNEDPxE1? M1H4PFczKGh? NYD3XpR1emWmdXPld{B1cGViZYjwdoV{e2mxbjDv[kBIdGlzwrC= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNzOUiyOEc,OjN|MUm4NlQ9N2F-
T315I BCR-ABL BaF3 M1O5O2Z2dmO2aX;uJGF{e2G7 MoDXNVAh|ryP MnfCO|IhcA>? MnTjdoVlfWOnczD0bIUh\XiycnXzd4lwdiCxZjDHcIkyyqB? MnfHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|MUm4NlQoRjJ|M{G5PFI1RC:jPh?=
TF-1 BCR-ABL Mnq5SpVv[3Srb36gRZN{[Xl? MVyxNEDPxE1? NULWUIxiPzJiaB?= Mon4doVlfWOnczD0bIUh\XiycnXzd4lwdiCxZjDHcIkyyqB? NUTjWVFYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzNVk5OjRpPkKzN|E6QDJ2PD;hQi=>
Shh-Light 2 NULxU2FbTnWwY4Tpc44h[XO|YYm= Ml7FNkBl[Xm| Ml;URY51[WexbnnzeEBi[3Srdnn0fUBifCCVbX:gbY4hdW:3c3WgV4hpNUyrZ3j0JFIh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDTbIgucW6mdXPl[EBIdGlzLYLldI9zfGW{IHHjeIl3cXS7IHHmeIVzKDJiZHH5d{BjgSCmdXHsMYx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDt[ZRpd2RuIFnDOVAhRSByLkCwNVUh|ryPLh?= Mkm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ|ME[zOVIzRC:jPh?=
NIH/3T3 M3HD[WZ2dmO2aX;uJIF{e2G7 MVTJcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDtc5V{\SCQSVivN3Q{KGOnbHzzJIJ6KEeuaT3keYFtNWy3Y3nm[ZJie2VicnXwc5J1\XJiYYPzZZktKEmFNUCgQUAxNjByMkOg{txONg>? M4n5TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUiwNFEzLz5{N{G4NFAyOjxxYU6=
NIH/3T3 Light2 Mmf3SpVv[3Srb36gZZN{[Xl? NFf1[I9KdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIF7JTE8{XDNiTHnnbJQzKGOnbHzzJIJ6KEeuaT3seYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUCgQUAxNjByMkOg{txONg>? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ6OEK3PEc,Ojh4OEiyO|g9N2F-
Light2 MkfBSpVv[3Srb36gZZN{[Xl? NILjUpU1QCCqcoO= NYXlbmRLUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDMbYdpfDJiY3XscJMh[W[2ZYKgOFghcHK|IHL5JGdtcS2udXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTBiPTCwMlAxOjR4IN88UU4> MoXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5M{m3NVQoRjJ7N{O5O|E1RC:jPh?=
HEPM MYHGeY5kfGmxbjDhd5NigQ>? MVLJcohq[mm2aX;uJI9nKFOKSDDpckBpfW2jbjDISXBOKGOnbHzzJIJ6KEeuaT3seYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZktKEmQSDC9JFAvODB{ODFOwG0v NEX4cWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUexOlI6Pid-MUm3NVYzQTZ:L3G+
Shh Light2 MkTmSpVv[3Srb36gZZN{[Xl? NYHFc4JPUW6qaXLpeIlwdiCxZjDTTGghcW5ibX;1d4UhW2iqIFzp[4h1OiClZXzsd{BjgSCJTFmtdoV{eG:wc3n2[UBncXKnZnz5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMECzJO69VS5? NUHJ[GtzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmzNFkxQDBpPkG5N|A6ODhyPD;hQi=>
HEPM MXnGeY5kfGmxbjDhd5NigQ>? MljZTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC|aXfuZYxqdmdiaX6gbJVu[W5iSFXQUUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5icnXj[ZB1d3JvbXXkbYF1\WRiR3zpNUBuWk6DIHX4dJJme3Orb36gZpkhemWyb4L0[ZIh\2WwZTDhd5NigSxiSV7IJF0hOC5yMEOg{txONg>? M3rPflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEe1O|QyLz5{MEi3OVc1OTxxYU6=
C3H10T1/2 MVXGeY5kfGmxbjDhd5NigQ>? MmrkNlAhcHK| NHr2T|BKdmirYnn0bY9vKG:oIGPtc{BqdiCvb4Xz[UBEO0hzMGSxM|Ih[2WubIOgeZNqdmdiaIXtZY4hemWlb33ibY5idnRiU1jIJIF{e2W|c3XkJIF{KGWoZnXjeEBwdiCVTV:vV2hJKHS{YX7zbYVvfCC2cnHud4NzcXC2aX;uZYwh[WO2aY\heIlwdiCjZoTldkAzOCCqcoOgZpkhT2yrLXz1Z4ln\XKjc3WgdoVxd3K2ZYKgZZN{[XluIFnDOVAhRSByLkCwOUDPxE1w NYf4bXlRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA1OzZpPkK0PVAxPDN4PD;hQi=>
NIH/3T3 NFHqRXRHfW6ldHnvckBie3OjeR?= M3LiW|I1KGi{cx?= NH7meIJKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIF7JTE8{XDNiY3XscJMhdWWjc4Xy[YQh[W[2ZYKgNlQhcHK|IHL5JGdtcS2mdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMEC1JO69VS5? M3nmWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OEGwOVkyLz5{N{ixNFU6OTxxYU6=
NIH3T3 M{HiPWZ2dmO2aX;uJIF{e2G7 M2XqXFQ5KGi{cx?= M4W4dWlvcGmkaYTpc44hd2Zic33vMY1m\GmjdHXkJIhm\GenaH;nJJNq\26jbHnu[{Bx[XSqd3H5JIlvKG2xdYPlJG5KUDOWMzDj[YxteyCneIDy[ZN{cW6pIFfSSU1NfWNicnXwc5J1\XJiZ3Xu[UBie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHNCTy2rbnT1Z4VlKEeURT3MeYMhemWyb4L0[ZIh[WO2aY\peJkh[W[2ZYKgOFghcHK|IHL5JIx2dWmwZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvODB4IN88UU4> MnTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OUm0PFMoRjJ7NEm5OFg{RC:jPh?=
Shh-light2 NWXqWmNFTnWwY4Tpc44h[XO|YYm= M3Tnd2lvcGmkaYTpc44hd2ZiU33vMY1m\GmjdHXkJGhpKHOrZ37hcIlv\yCrbjDoeY1idiCVaHitcIlocHR{IHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvODB5IN88UU4> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4OEW1NUc,OjJ{Nki1OVE9N2F-
HEK293 MXTGeY5kfGmxbjDhd5NigQ>? NG\Fc5UzKGi{cx?= MVTEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NWyjYnXscIVlKGO7Y3zvdIFucW6nIH\yc40hcHWvYX6gV41wKHKnY3XweI9zKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[W[2ZYKgNkBpenNiYomg[ox2d3Knc3PlcoNmKG2rY4Lvd4NweHluIFnDOVAhRSByLkCwO{DPxE1w M4C1O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[4OVUyLz5{MkK2PFU2OTxxYU6=
NIH3T3 NIXLdHJHfW6ldHnvckBie3OjeR?= MYrJcohq[mm2aX;uJI9nKGinZHflbI9oKHKnY3XweI9zKHOrZ37hcIlv\yCyYYToe4F6KGmwIH3veZNmKE6LSEPUN{Bk\WyuczD0doFve2[nY4Tl[EB4cXSqIFfsbU1z\XCxcoTldkBo\W6nIHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMEC3NVch|ryPLh?= NWezb25ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NlM4PjVpPkK0PVI{PzZ3PD;hQi=>
NIH/3T3 NX;Lc|h5TnWwY4Tpc44h[XO|YYm= NIqwbow1QCCqcoO= NWLt[JFEUW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBUd26rYzDo[YRo\WixZz3pcoR2[2WmIFfsbUBtfWOrZnXyZZNmKGGldHn2bZR6KGGodHXyJFQ5KGi{czDifUBFfWGuLXz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvODB5MUeg{txONg>? M1nyfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMkS5OFk1Lz5|MEK0PVQ6PDxxYU6=
NIH3T3 MVfGeY5kfGmxbjDhd5NigQ>? NFn6XYU1QCCqcoO= NUjXcVJxUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyuaX7nJIlvKG2xdYPlJG5KUDOWMzDj[YxteyC|dHHicJkhfHKjboPm[YN1\WRid3n0bEBIdGlvbIXjbYZmemG|ZTDjc45{fHK3Y4SgbY5kfWKjdHXkJIZweiB2ODDodpMh[nliZIXhcEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVAhRSByLkCwO|Ih|ryPLh?= Mnr2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ6MkexN|YoRjJ4OEK3NVM3RC:jPh?=
NIH3T3 M3LiN2Z2dmO2aX;uJIF{e2G7 NVvHWpZiPDhiaILz Mn\4TY5pcWKrdHnvckBw\iCVb37pZ{1qdmS3Y3XkJIhm\GenaH;nJJNq\26jbHzpcochcW5ibX;1d4UhVkmKM2SzJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCJbHmtcJVkcW[ncnHz[UBz\XCxcoTldkBie3OjeTygTWM2OCB;IECuNFA4OiEQvF2u NUX4bXV7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4NlA2PTRpPkK2PFIxPTV2PD;hQi=>
NIH3T3 MUjGeY5kfGmxbjDhd5NigQ>? MonpOFghcHK| NHzTSo5KdmirYnn0bY9vKG:oIGPITEB{cWewYXzpcocheGG2aIfhfUBqdiCvb4Xz[UBPUUh|VEOgZ4VtdHNibXXhd5Vz\WRiYX\0[ZIhPDhiaILzJIJ6KEeuaT3seYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7LDDJR|UxKD1iMD6wNFczKM7:TT6= Mn\5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzN{[zPVYoRjJ2MUe2N|k3RC:jPh?=
NIH/3T3 NVH2cnM6TnWwY4Tpc44h[XO|YYm= MmjCOFghcHK| NEe1TGFKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIF7JTE8{XDNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JGdtcS2udXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTBiPTCwMlAxPzJizszNMi=> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR6NkKwN{c,OjR2OE[yNFM9N2F-
NIH/3T3 NUH0NJBGTnWwY4Tpc44h[XO|YYm= M37QZ2lvcGmkaYTpc44hd2ZiaHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gcY92e2ViTlnIM|NVOyClZXzsd{BjgSCJbHmxMYx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwMEC3NkDPxE1w NX3BXYE{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NFU4ODRpPkK0OFA2PzB2PD;hQi=>
NIH/3T3 NYDyRmM3TnWwY4Tpc44h[XO|YYm= NYTSToFMUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGOjcoL5bY5oKHO2YXLsfUB1emGwc3\lZ5Rm\CCJbHmtdoVxd3K2ZYKgZ49ve3S{dXP0JIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiYYPzZZktKEmFNUCgQUAxNjByN{Kg{txONg>? NUKydZI6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OFIyODFpPkK4OlQzOTBzPD;hQi=>
C3H10T1/2 NX;Ue241TnWwY4Tpc44h[XO|YYm= NHzsd|U3KGi{cx?= MoK2TY5pcWKrdHnvckBw\iCVQVetbY5lfWOnZDDkbYZn\XKnboTpZZRqd25ib3[gcY92e2VibXXz[Y5kcHmvYXygdIx2emmyb4TlcpQhSzOKMUDUNU8zKGOnbHzzJJRwKGGua3HsbY5mKHCqb4PwbIF1[XOnIIDvd4l1cX[nIH;ld4Vw[myjc4TzJIFnfGW{IE[gbJJ{NCCLQ{WwJF0hOC5yMUGg{txONg>? M3i2d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[4OVUyLz5{MkK2PFU2OTxxYU6=
HCC827 M{L6WGZ2dmO2aX;uJIF{e2G7 MUfEbZNxdGGlZX3lcpQhd2ZiW{PIYU1kgWOub4DhcYlv\SCocn;tJHNOVyCYNEC0UUBufXSjboSgbY4h\2WoaYTpcoljKHKnc3nzeIFvfCCqdX3hckBJS0N6MkegZ4VtdHNiYomgd4NqdnSrbHzheIlwdiClb4XueIlv\yxiS3mgQUAxNjBzMkKg{txONg>? MkHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5OEexOVYoRjJ6N{i3NVU3RC:jPh?=
C3H10T1/2 M3rjTGZ2dmO2aX;uJIF{e2G7 MUfJcohq[mm2aX;uJI9nKFOKSDDpckBud3W|ZTDDN2gyOFRzL{KgZ4VtdHNiYomgS4xqNWy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuNFE{KM7:TT6= NXPybmFlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm3NVYzQTZpPkG5O|E3Ojl4PD;hQi=>
S12 NHPqXGtHfW6ldHnvckBie3OjeR?= NVLHb5J1UW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegbY4hdW:3c3WgV|EzKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDy[YNmeHSxcj3t[YRq[XSnZDDHcIkyKG2UTlGg[ZhxemW|c3nvckBjgSC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJUmghRSByLkCxN{DPxE1w NUHPOldbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4O|U4PDFpPkKwPFc2PzRzPD;hQi=>
TM3 NUPkR2R{TnWwY4Tpc44h[XO|YYm= NYXPNFZLPDhiaILz MUnJcohq[mm2aX;uJI9nKEiqIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIGTNN{Bk\WyuczDhd5Nme3OnZDDhd{Bld3ewcnXneYxifGmxbjDv[kBIdGlzIHflcoUh\XiycnXzd4lwdiCjZoTldkA1QCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDFR|UxKD1iMD6wNVMh|ryPLh?= M4P6RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUe2NlE2Lz5{Nkm3OlIyPTxxYU6=
NIH/3T3 NF;s[mhHfW6ldHnvckBie3OjeR?= MW\Jcohq[mm2aX;uJI9nKGinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6KGmwIH3veZNmKE6LSD:zWFMh[2WubIOg[ZhxemW|c3nu[{B4cWymIIT5dIUhW22xIHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCJbHmgcXJPSSCneIDy[ZN{cW:wIHL5JHJVNVCFUjDt[ZRpd2RuIFnDOVAhRSByLkCxOFQh|ryPLh?= M2\wclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5OEGwOVkyLz5{N{ixNFU6OTxxYU6=
S12 NVPOSJBGTnWwY4Tpc44h[XO|YYm= NEezd|E1QCCqcoO= NX3qN|NmUW6qaXLpeIlwdiCxZjDoeY1idiCVSFigdIF1cHejeTDpckBud3W|ZTDTNVIh[2WubIOgZZN{\XO|ZXSgZZMhT0yLLX3l[IlifGWmIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCjZoTldkA1QCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxKD1iMD6wNVUh|ryPLh?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR|OEWyO{c,OjF2M{i1Nlc9N2F-
NIH/3T3 M1LQbmZ2dmO2aX;uJIF{e2G7 NWDyVVZNUW6qaXLpeIlwdiCxZjDTUW8hcW5ibX;1d4UhVkmKL{PUN{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGPBS{1qdmS3Y3XkJIhm\GenaH;nMY1m\GmjdHXkJIx2dWmwZYPj[Y5k\SC|aXfuZYxqdmdiYomgS3JGNWy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuNFE3KM7:TT6= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjFzOUWwNEc,OjZzMUm1NFA9N2F-
U2OS MUjGeY5kfGmxbjDhd5NigQ>? Ml\3NkBpenN? NX7EeZdnTGm|cHzhZ4Vu\W62IH;mJHs{UF2leXPsc5BidWmwZTDmdo9uKHerbHSgeJlx\SCVbX:g[ZhxemW|c3XkJIlvKFV{T2OgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4khRSByLkCxOlIh|ryPLh?= M4nUUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[zOVIzLz5{M{C2N|UzOjxxYU6=
NIH3T3 NWjxPId[TnWwY4Tpc44h[XO|YYm= Mn[xN|AhcHK| MXLJcohq[mm2aX;uJI9nKFOvbzDy[YNmeHSxcjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBPUUh|VEOgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCVbX:gZYdwdmm|dDDTRWcucW6mdXPl[EBIWkViYXP0bZZifGmxbjDh[pRmeiB|MDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyIE2gNE4xOjNizszNMi=> NWS5TnBxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NlY5ODdpPkK0O|I3QDB5PD;hQi=>
medulloblastoma cell MWHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MVOzOkBpenN? NVG5OY1XSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBRfGOqKz:tJIFv\CCyNUOtM{0hdW:3c3WgcYVlfWyub3LsZZN1d22jIHPlcIx{KGGodHXyJFM3KGi{czDifUBDemS3IHnuZ49zeG:{YYTpc44h[XO|YYmsJGlEPTBiPTCwMlA{ODRizszNMi=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ6OEK3PEc,Ojh4OEiyO|g9N2F-
Shh Light2 NVmwW3l2TnWwY4Tpc44h[XO|YYm= M1HkdWFvfGGpb37pd5Qh[WO2aY\peJkh[XRic33vc5Rp\W6nZDCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBud3W|ZTDTbIghVGmpaISyJINmdGy|IHPvMYV5eHKnc4PpcochT2yrLXTldIVv\GWwdDDy[ZBwenSncjDn[Y5mKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhUGhic3nncoFtcW6pIHL5JIR2[WxibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyIE2gNE4xOzNizszNMi=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR7MUS1PUc,OjR2OUG0OVk9N2F-
Light2 MWfGeY5kfGmxbjDhd5NigQ>? MmG5TY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCOaXfoeFIh[2WubIOgbY4hW2iqIHPvcoRqfGmxbnXkJI1m\Gm3bTDifUBIdGlvbIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyIE2gNE4xOzlizszNMi=> MmThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6N{OzNFMoRjJ6OEezN|A{RC:jPh?=
Shh Light2 MoHwSpVv[3Srb36gZZN{[Xl? NI\PXJhKdmirYnn0bY9vKG:oIGPtc{1u\WSrYYTl[EBJcCC|aXfuZYxtcW6pIIDheIh4[XliaX6gcY92e2ViU3joJGxq\2i2MjDj[YxteyCkeTDHcIkudHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR|UxKD1iMD6wN|kzKM7:TT6= M1W2VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{O2NFY{Lz5{N{ezOlA3OzxxYU6=
ASZ001 MnzjSpVv[3Srb36gZZN{[Xl? NHjiRmo1QCCqcoO= NXu4S4ZqUW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegbY4hdW:3c3WgRXNbODBzIHPlcIx{KGG|c3Xzd4VlKGG|IHTve45z\We3bHH0bY9vKG:oIFfsbVEhdVKQQTDlfJBz\XO|aX;uJIFnfGW{IES4JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXNuIFnDOVAhRSByLkC0JO69VS5? MoCxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3NVYoRjJ2OUCwO|E3RC:jPh?=
NIH/3T3 MYTGeY5kfGmxbjDhd5NigQ>? M4HZS|Q5KGi{cx?= M{TWXGFvfGGpb37pd5Qh[WO2aY\peJkh[XRiU33vJJJm[2WydH;yJIlvKG2xdYPlJG5KUC9|VEOgZ4VtdHNiaHHyZo9zcW6pIFfSSU1NfWNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCVQVetbY5lfWOnZDDIbEB{cWewYXzpcocheGG2aIfhfUBxemWrbnP1ZoF1\WRid3n0bEBk\WyuczDmc4xtd3enZDDifUBUSUdiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhPDhiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuNFQ3KM7:TT6= NWP1c3RURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm4OVczPzVpPkK5PFU4Ojd3PD;hQi=>
Light2 MV3GeY5kfGmxbjDhd5NigQ>? MWSzNEBpenN? NEXJPIpKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIFzp[4h1OiClZXzsd{BqdiCVaHigZ49v\Gm2aX;u[YQhdWWmaYXtJIFnfGW{IEOwJIhzeyCkeTDHcIkuWmWwaXzsZUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVAhRSByLkC1JO69VS5? NYLCfoM{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwPVkzPTdpPkOwNFk6OjV5PD;hQi=>
C3H10T1/2 M1zQNGZ2dmO2aX;uJIF{e2G7 NULmfYJzPiCmYYnz Mn7vTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigS2vZXTpZZRm\CCmaX\m[ZJmdnSrYYTpc44hd2ZibX;1d4UhSzOKMUDUNU8zKGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIGPBS{1qdmS3Y3XkJGFNWCCjY4Tpeol1gSCjZoTldkA3KGSjeYOgZpkh[2inbXnseY1qdmW|Y3XuZ4Uu[mG|ZXSgZZN{[XluIFnDOVAhRSByLkC1JO69VS5? M{Lv[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyMEm5NlU4Lz5|MEC5PVI2PzxxYU6=
DaOY M1HyVWZ2dmO2aX;uJIF{e2G7 NGLTNZM1QCCqcoO= NIPpTlVKdmirYnn0bY9vKG:oIFjl[IdmcG:pIIPp[45idGmwZzDpckBpfW2jbjDEZW9[KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaUGgcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDR6IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjB6NjFOwG0v MoDiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3NVYoRjJ2OUCwO|E3RC:jPh?=
C3H10T1/2 MVnGeY5kfGmxbjDhd5NigQ>? NXz6TVVbOSCqch?= M{KwNGlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBxfXKvb4LwbIFucW6nLXnu[JVk\WRiaX7jdoVie2ViaX6gZYxs[WyrbnWgdIhwe3CqYYThd4UhdGW4ZXygeZNqdmdiQ1TQMZN1[XJiYYOgd5Vje3S{YYTlJJBz\XS{ZXH0[YQh\m:{IEGgbJIh\m:ubH;3[YQh[nlicIXycY9zeGijbXnu[UBi\GSrdHnvckBu\WG|dYLl[EBi\nSncjC1JIRigSxiSVO1NEA:KDBwMUSg{txONg>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDl2N{mzPUc,Ojh7NEe5N|k9N2F-
M210B4 M3foOGZ2dmO2aX;uJIF{e2G7 MWqyOEBpenN? Mn;OTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC|aXfuZYxqdmdiaX6gcY92e2ViTUKxNGI1KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKFC2Y3igcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjF2IN88UU4> MlH2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3NVYoRjJ2OUCwO|E3RC:jPh?=
M210B4 MYPGeY5kfGmxbjDhd5NigQ>? M1XFTVI1KGi{cx?= M{L1VGlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIHnuJI1wfXOnIF2yNVBDPCClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCJbHmxJI1TVkFiZYjwdoV{e2mxbjDh[pRmeiB{NDDodpMh[nliUmStVGNTKGGwYXz5d4l{NCCLQ{WwJF0hOC5{IN88UU4> M13Y[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwO|E3Lz5{NEmwNFcyPjxxYU6=
Shh Light2 NIS0TnpHfW6ldHnvckBie3OjeR?= MmnENUBpeg>? MWPJcohq[mm2aX;uJI9nKEinZHflbI9oKHOrZ37hcIlv\yCrbjDtc5V{\SCVaHigUIlocHR{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBxfXKvb4LwbIFucW6nLXnu[JVk\WRiR3zpJI1m\GmjdHXkJJRz[W6|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDwdoV1emWjdHXkJIZweiBzIHjyJIZwdGyxd3XkJIJ6KHC3cn3vdpBp[W2rbnWgZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiNEigbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvQThizszNMi=> NX;aS5VLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5OFc6OzlpPkK4PVQ4QTN7PD;hQi=>
NIH/3T3 NIPQZmdHfW6ldHnvckBie3OjeR?= NVnxbHFUUW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDOTWgwO1R|IHPlcIx{KGijcnLvdolv\yCVbX:gSFQ4P0hibYX0ZY51KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBIdGlibWLORUBmgHC{ZYPzbY9vKGK7IGLUMXBEWiCvZYToc4QtKEmFNUCgQUAyNjF7NjFOwG0v NEnzWoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{ixNFU6OSd-Mke4NVA2QTF:L3G+
S12 MnHNSpVv[3Srb36gZZN{[Xl? NF74R3U1QCCqcoO= MkPlTY5pcWKrdHnvckBw\iCqdX3hckBUUEhicHH0bJdigSCrbjDtc5V{\SCVMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgS2xKNW2nZHnheIVlKHS{YX7zZ5JqeHSrb37hcEBi[3Srdnn0fUBi\nSncjC0PEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFAvPSCvZz;tUEBpfW2jbjDhcJBp[S1zLXHjbYQh\2y7Y3;wdo91\WmwLDDJR|UxKD1iMT63OUDPxE1w NF\5RYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUSzPFUzPyd-MkG0N|g2Ojd:L3G+
Calu6 M3q0OGZ2dmO2aX;uJIF{e2G7 MonXOEBpenN? NW\SWpo6WGyjc33hJINwdmOnboTyZZRqd25iaX6gbJVu[W5iQ3HseVYh[2WubIOgfIVvd2e{YX\0[YQhcW5iboXk[UBud3W|ZTDheEA4PSCvZz;r[{wheG9iYnnkJI1m[XO3cnXkJIFnfGW{IESgbJJ{KHCxc4Sg[olnfGhiZH;z[UwhS3BiPTCyN{DPxE1w Mn\wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5MU[yPVYoRjF7N{G2Nlk3RC:jPh?=
Calu-6 NIr4b5RHfW6ldHnvckBie3OjeR?= MlfIVIxie22jIHPvcoNmdnS{YYTpc44hcW5iQ3HseU03KGOnbHzzJJhmdm:pcnHmeIVlKG63ZHWgcY92e2ViUFuvVGQhdW:mZXygZZQhPzVibXevb4ctKHCxIHLp[EBnd3JiNTDkZZl{KG2nYYP1doVlKDRiaILzJJBwe3RibHHzeEBld3OnIILlcIF1cX[nIITvJJVvfHKnYYTl[EBkd262cn;sMEBEeCB;IEKzJO69VS5? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh5NUe0NUc,OjB6N{W3OFE9N2F-
T47D MUfDfZRwfG:6aXPpeJkh[XO|YYm= MV[3NkBpenN? MYTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDUOFdFKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC0NU4{PCEQvF2u MmDsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzMEe0NlkoRjJ7MUC3OFI6RC:jPh?=
LS180 M4q3SWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGrkdXA4OiCqcoO= NFXCbpVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzTNVgxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDzeYxnd3Kqb3ThcYlv\S2EIHHzd4F6NCCLQ{WwJF0hPDVizszNMi=> MkfVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByOUmyOVcoRjNyMEm5NlU4RC:jPh?=
LS174T NETqeI5CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MYe3NkBpenN? MmDtRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOU{G3OHQh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPlcIxVcXSncj3HcI8hdHWvaX7ld4NmdnRiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTBiPTC0OU45OSEQvF2u MnnUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ6MkC1OVQoRjJ4OEKwOVU1RC:jPh?=
BxPC3 Mk\CRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MX:3NkBpenN? MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEK6UFOzJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCFZXzsWIl1\XJvR3zvJIx2dWmwZYPj[Y51KGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxKD1iNEeuPVUh|ryPLh?= NWfyNIo1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4NlA2PTRpPkK2PFIxPTV2PD;hQi=>
medulloblastoma cell M3ftWmFvfGm2dX3vdkBie3OjeR?= NFfjNVZCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBud3W|ZTDt[YR2dGyxYnzhd5RwdWFiY3XscJMhgGWwb3fyZYZ1\WRiaX6gVJRkcCBtLz2gcY92e2ViYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hfHWvb4Kgeo9tfW2nIHH0JFEzNjVibXevb4ctKHCxIHLp[E4> MoTTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl5MU[yPVYoRjF7N{G2Nlk3RC:jPh?=
S12 NHLIc4ZHfW6ldHnvckBie3OjeR?= NWfvfoFuPDhiaILz NX;jU5hnUW6qaXLpeIlwdiCxZjDoeY1idiCVSFigdIF1cHejeTDpckBud3W|ZTDTNVIh[2WubIOgZZN{\XO|ZXSgZZMhT0yLLX3l[IlifGWmIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCjZoTldkA1QCCqcoOgZpkhdHWlaX\ldoF{\SC{ZYDvdpRmeiCpZX7lJIF{e2G7IHnuJJBz\XOnbnPlJI9nKDFibXevcWwhcHWvYX6gZYxxcGFvMT3hZ4llKGeueXPvdJJwfGWrbj6= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTR|OEWyO{c,OjF2M{i1Nlc9N2F-
Shh Light2 MWHGeY5kfGmxbjDhd5NigQ>? MlS4NE4yKHWP MlvYN|YhcHK| M1;1bGlvcGmkaYTpc44hd2ZiU33vMY1m\GmjdHXkJGhpKHOrZ37hcIxqdmdicHH0bJdigSCrbjDtc5V{\SCVaHigUIlocHR{IHPlcIx{KGG|c3Xzd4VlKGG|IIP1dJBz\XO|aX;uJI9nKEeuaUGtcXJPSSCneIDy[ZN{cW:wIHH0JFAvOSC3TTDt[YF{fXKnZDDh[pRmeiB|NjDodpMh[nliUmStdXBEWiCjbnHsfZNqey5? M4TndVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{O2NFY{Lz5{N{ezOlA3OzxxYU6=
SW1353 MnvjRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NUmyTXRKOTBidV2= MojJOFghcHK| NYmwWW9VSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTW|E{PTNiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYYSgNVAhfU1iYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigS5? NGPYTY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES5NVQ2QSd-MkS0PVE1PTl:L3G+
DaOY M1fm[2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 M1foWlExKHWP NHzC[5I1QCCqcoO= NHfDWGVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFThU3kh[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[XRiMUCgeW0h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfU4> NW[ySmNNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0PVE1PTlpPkK0OFkyPDV7PD;hQi=>
A549 Mm\JRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M2ficFExKHWP NXjxb2JZPDhiaILz MVHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOg[5Jwf3SqIHnubIljcXSrb36gZZQhOTBidV2gZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeT6= M{fBUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEmxOFU6Lz5{NES5NVQ2QTxxYU6=
HCT116 M3HoUGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NFrFV2oyOCC3TR?= NYfDSIRnPDhiaILz Mne0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO|ZXSgZZMh\3Kxd4ToJIlvcGmkaYTpc44h[XRiMUCgeW0h[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfU4> NHLkZZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES5NVQ2QSd-MkS0PVE1PTl:L3G+
NIH/3T3 NYrVR21JTnWwY4Tpc44h[XO|YYm= MnnXNVAhfU1? NVjFXJRQUW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegbY4hdW:3c3WgUmlJNzOWMzDj[YxteyCjc4Pld5Nm\CCjczDkc5dvKHKnZ4XsZZRqd25ib3[gcXBVS0hzIH3SUmEh\XiycnXzd4lwdiCjdDCxNEB2VS5? NU\iSVM3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0PVE1PTlpPkK0OFkyPDV7PD;hQi=>
MSC M4LuV2Z2dmO2aX;uJIF{e2G7 M4iyNlIxKHSxIEiwJJVO NHvrO21KdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDpckBud3W|ZTDNV2Mh[2WubIOgZZN{\XO|ZXSgZZMh\G:5bjDy[Yd2dGG2aX;uJI9nKGGua3HsbY5mKHCqb4PwbIF1[XOnIHH0JFIxKHSxIEiwJJVONg>? NGq4[5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES5NVQ2QSd-MkS0PVE1PTl:L3G+
SK-N-MC MV7xTHRUKGG|c3H5 MVXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucz6= NIDjWos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
TC32 M2DibJFJXFNiYYPzZZk> MXfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDUR|MzKGOnbHzzMi=> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NIH3T3-Luc MonuSpVv[3Srb36gZZN{[Xl? M3rxWFEhfU1? MV20PEBpenN? NGHWZZZKdmirYnn0bY9vKG:oIIPtc{1u\WSrYYTl[EBp\WSpZXjv[{B{cWewYXzpcocheGG2aIfhfUBqdiCvb4Xz[UBPUUh|VEOtUJVkKGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaTDtVm5CKGW6cILld5Nqd25iYYSgNUB2VSCjZoTldkA1QCCqcoOgZpkhemWjbD30bY1mKFCFUjDhcoFtgXOrcz6= NVOzRWdGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0PVk1QDNpPkK5OFk6PDh|PD;hQi=>
C3H10T1/2 MmKxSpVv[3Srb36gZZN{[Xl? MUKyOEBpenN? MmnjTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigS2vZXTpZZRm\CCJTFmxJI1TVkFiZYjwdoV{e2mxbjDpckBud3W|ZTDDN2gyOFRzL{KgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KFKWLWDDVkBidmGueYPpd{BqdiCycnXz[Y5k\SCxZjCoNXItO2GULETTMFdiWilvN3GtcYV1cHmuLUGtLEhTMS14LX3leIh6dGincIThck0zNXmuKX;jeIFpgWS{bz2xTE1qdmSnbj20MZltKDNvaInkdo95gWKnborvZZRmNg>? NXzPZlloRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3N|A6QDRpPkK0O|MxQTh2PD;hQi=>
C3H10T1/2 Ml3JSpVv[3Srb36gZZN{[Xl? NVzMTZhROjRiaILz M4fVcmlvcGmkaYTpc44hd2ZiaHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XlvbXXkbYF1\WRiR1zJNUBuWk6DIHX4dJJme3Orb36gbY4hdW:3c3WgR|NJOTCWMT:yJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMhcW5icILld4Vv[2Vib3[gWmQ{Ng>? M3v2R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{OwPVg1Lz5{NEezNFk5PDxxYU6=
NIH/3T3 NGnoXY9HfW6ldHnvckBie3OjeR?= NXLNc40yOTByIH7N NXnRUHBtOzZiaILz MnG1TY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCQSVivN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKFOqaD3pcoR2[2WmIFfsbVEhdVKQQTDlfJBz\XO|aX;uJIxmfmWuczDheEAyODBibl2gZYZ1\XJiM{[gbJJ{KGK7IGLUMZFRS1JiYX7hcJl{cXNw NXrX[ZlERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4O|M{ODNpPkK4PFc{OzB|PD;hQi=>
C3H10T1/2 NXvIeIpxTnWwY4Tpc44h[XO|YYm= NVHKeIlFOTByIH7N NUnjVZh1OzZiaILz Mn7rTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCFM1ixNHQyNzJiY3XscJMh[XO|ZYPz[YQh[XNiZH;3cpJm\3WuYYTpc44hd2ZiU3joMYlv\HWlZXSgS4xqOSCvUl7BJIV5eHKnc4Ppc44hdGW4ZXzzJIF1KDFyMDDuUUBi\nSncjCzOkBpenNiYomgVnQueVCFUjDhcoFtgXOrcz6= M1jsclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEezN|A{Lz5{OEi3N|MxOzxxYU6=
NIH/3T3 M2HwT2Z2dmO2aX;uJIF{e2G7 MofGNVAxKG6P MkPWN|YhcHK| NFXpXHRKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIF7JTE8{XDNiY3XscJMh[XO|ZYPz[YQh[XNiZH;3cpJm\3WuYYTpc44hd2ZiU3joMYlv\HWlZXSgdJRkcDFibWLORUBmgHC{ZYPzbY9vKGyndnXsd{BifCBzMECgcm0h[W[2ZYKgN|YhcHK|IHL5JHJVNXGSQ2KgZY5idHm|aYOu M4iwUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OEezN|A{Lz5{OEi3N|MxOzxxYU6=
C3H10T1/2 MoGxSpVv[3Srb36gZZN{[Xl? NEH3fWIyODBibl2= NFG0b3c{PiCqcoO= NWLuXYQ5UW6qaXLpeIlwdiCxZjDo[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBud3W|ZTDDN2gyOFRzL{KgZ4VtdHNiYYPz[ZN{\WRiYYOg[I94dnKnZ4XsZZRqd25ib3[gV4hpNWmwZIXj[YQheHSlaEGgcXJPSSCneIDy[ZN{cW:wIHzleoVteyCjdDCxNFAhdk1iYX\0[ZIhOzZiaILzJIJ6KFKWLYHQR3Ih[W6jbInzbZMv MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh5M{OwN{c,Ojh6N{OzNFM9N2F-
medulloblastoma cell NIDNbodCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MUH1dEB1dyB|MDD1US=> NXrLTm1TPzJiaILz NUjVd5VUSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDQeINpMy9vIIC1N{0wNSCvZXT1cIxw[myjc4TvcYEh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwheHKxbHnm[ZJifGmxbjD1dEB1dyB|MDD1UUBi\nSncjC3NkBpenNiYomgUXRUKGG|c3H5Mi=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh5M{OwN{c,Ojh6N{OzNFM9N2F-
medulloblastoma cell M{noU2Fxd3C2b4Ppd{Bie3OjeR?= NInEb4VKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJI1wfXOnIGD0Z4gsNy1icEWzMU8uKG2nZIXscI9jdGG|dH;tZUBk\WyuczDpcZBt[W62ZXSgbY4h[XSqeX3pZ{BvfWSnIH3veZNmKGG2IEGyMlUhdWdxa3esJIlxKGKrZDDmc5IhOTViY3;ud4VkfXSrdnWg[IF6eyCkeTDUWW5GVCCjc4PhfU4> NIjkc5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEi3N|MxOyd-Mki4O|M{ODN:L3G+
NIH/3T3 NGTPXVhHfW6ldHnvckBie3OjeR?= M4HtS2lvcGmkaYTpc44hd2ZiaHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliZYjwdoV{e2WmIHnuJI1wfXOnIF7JTE8{XDNiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBp\WSpZXjv[{1qdmS3Y3XkJGdtcS1{IHHjZ5VufWyjdHnvckBifCC2aYCgc4YheHKrbXHyfUBkcWyrYTDifUBFSVCLIIP0ZYlvcW6pIHLhd4VlKGOxbn\vZ4FtKG2rY4Lvd4NweGmlIHHuZYx6e2m|Lh?= NF6wWIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{ixNFU6OSd-Mke4NVA2QTF:L3G+
NIH/3T3 NE\adWZHfW6ldHnvckBie3OjeR?= MkHxTY5pcWKrdHnvckBw\iCqZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCQSVivN3Q{KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gbIVl\2Wqb3etbY5lfWOnZDDTcY8uTUeIUDDjbYxq[XK7IITyZY5{dG:lYYTpc44h[nliRFHQTUB{fGGrbnnu[{Bj[XOnZDDjc45nd2OjbDDtbYNzd3Olb4DpZ{BidmGueYPpd{4> MkPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd6MUC1PVEoRjJ5OEGwOVkyRC:jPh?=
medulloblastoma cell NInZUlNCdnSrLYT1cY9zKGG|c3H5 NUXFbow4SW62aT30eY1weiCjY4Tpeol1gSCjZ3HpcpN1KG2xdYPlJHB1[2htLz2gdFU{NS9vIH3l[JVtdG:kbHHzeI9u[SClZXzsd{BqdXCuYX70[YQhcW5iYYTofY1q[yCwdXTlJI1wfXOnIHHzd4V{e2WmIHHzJJR2dW:{IHfyc5d1cCCrbnjpZol1cW:wIHH0JFIxKG2pL3vnMEBxdyCjZH3pcol{fGW{ZXSgeJdq[2ViZHHpcJkhfmmjIHfheoFo\SCvZXHzeZJm\CCndnXyfUBwfGincjDkZZkh\HW{aX7nJINwdXCxdX7kJIRwe2mwZz6= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd|OUexOEc,Ojl5M{m3NVQ9N2F-
C3H10T1/2 MorzSpVv[3Srb36gZZN{[Xl? M1rENFQvOSC2bzCxNFAxKG6P NGjRWWdCdGyxc4TldolkKGmwaHnibZRqd25ib3[gV41wKGmwIH3veZNmKEN|SEGwWFEwOiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJB2em2xcoDoZY1qdmVvaX7keYNm\CCJbHmxJJRz[W6|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDheEA1NjFidH:gNVAxOCCwTTDifUByWEO{IHHuZYx6e2m|Lh?= NFHsdG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C3OFU1OSd-MkOwO|Q2PDF:L3G+
C3H10T1/2 MnHqSpVv[3Srb36gZZN{[Xl? NWPYfYJ6PC5zIITvJFExODBibl2= NXnyeWVLS2:vcHX0bZRqfmViaX7obYJqfGmxbjDv[kBUdW9iaX6gcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhW0GJLXnu[JVk\WRiR3zpNUB1emGwc3PybZB1cW:wYXygZYN1cX[rdImgZZQhPC5zIITvJFExODBibl2gZpkheVCFUjDhcoFtgXOrcz6= NUDLUFgyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO|Q2PDFpPkKzNFc1PTRzPD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Fas / DR4 / DR5 / Cleaved PARP / Bcl-2 / Cleaved caspase-3 / PDGFRα; 

PubMed: 22087285     


Pancreatic CSCs were treated with GDC-0449 (0, 1, 5 and 10 µM) for 48 h. The expression of Fas, DR4/TRAIL-R1, DR5/TRAIL-R2, PARP cleavage, Bcl-2, and Caspase-3 by the Western blot analysis. β-Actin was used as a loading control.

p-GSK3β / GSK3β / p-Akt / Akt / Gli1; 

PubMed: 27143997     


Cells were pretreated with or without 30 μM GDC-0449 for 2 h before TMT treatment. After incubation with 3.75 μM TMT for another 24 h, the expression levels of proteins were determined through Western blot analysis.

Gli1 / SOX2 / OCT4; 

PubMed: 26418365     


The activation of Hedgehog pathways and CSC markers were tested by immunoblotting analysis in Hs578T and BT549 cells. β‐Actin was used as a loading control.

p53 / Cyclin D1 / p21; 

PubMed: 28195165     


GDC-0449 treatment affected the expression levels of cell cycle-related protein levels. U251 cells were treated with 0.1% DMSO or GDC-0449 at the indicated concentrations for 24 h. Cells were then harvested and examined using Western blot analysis with the indicated antibodies.

Bcl-2 / Bax; 

PubMed: 28195165     


GDC-0449 treatment affected the expression levels of cell cycle-related protein levels. U251 cells were treated with 0.1% DMSO or GDC-0449 at the indicated concentrations for 24 h. Cells were then harvested and examined using Western blot analysis with the indicated antibodies.

22087285 27143997 26418365 28195165
Immunofluorescence
Gli1 / Gli2; 

PubMed: 22087285     


GDC-0449 inhibits expression of Gli1 and Gli2 in human pancreatic CSCs. The cells were seeded on fibronectin-coated coverslips and treated with GDC-0449 (10 µM) for 48 h. Subsequently, cells were fixed with 4% paraformaldehyde, blocked in 10% normal goat serum and stained with Gli1 and Gli2 primary antibodies (1∶100) for 16 h at 4°C and washed with PBS. Afterwards, cells were incubated with fluorescently labeled secondary antibody (1∶200) along with DAPI (1 mg/ml) for 1 h at room temperature and cells were mounted and visualized under a fluorescent microscope. For better visuality, the color of DAPI was changed from blue to red.

22087285
Growth inhibition assay
Cell viability; 

PubMed: 29042665     


GDC-0449 improves the anti-proliferation activity in NIH-3T3 cells but not in BxPC-3, Panc-1, MIAPaca-2 and SW1990 cells when cultured alone. The cytotoxicities of GDC-0449, Dox or Dox with GDC-0449 (5 μM or 10 μM) were measured by performing a 48-h MTT assay in BxPC-3 (A), Panc-1 (B), MIAPaca-2 (C), SW1990 (D) and NIH-3T3 (E) cells. Apoptosis rate was detected by flow cytometry (Annexin V staining) analysis after treatment with Dox (200 nM) with or without GDC-0449 for 24 h (F). Data are presented as the mean ± SD. *P < 0.05, **P < 0.01.

29042665
体内研究 GDC-0449已经用于治疗动物模型的成髓细胞瘤。[2] GDC-0449 抑制原代胰腺移植瘤生长,但是不抑制细胞增殖。GDC-0449按25 mg/kg 以上剂量口服给药髓母细胞瘤Ptch(+/-)异体移植物模型,引起细胞衰退,按92 mg/kg剂量处理两种配位体依赖的结肠直肠癌模型D5123和1040830,每天处理两次,抑制肿瘤生长。分析Hh通路活性和 PK/PD 模型显示GDC-0449抑制 Gli1,IC50值与GDC-0449作用于髓母细胞瘤和D5123模型的IC50值差不多(分别为0.165 μM ±11.5% 和0.267 μM ±4.83%)。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[2]
- 合并
  • Cell lines: MDCKII细胞
  • Concentrations: 20 μM
  • Incubation Time: 2小时
  • Method: MDCKII细胞按每孔3x105密度接种在24孔板中。培养液包含不同药物,实验组为50 µM VP,50 µM吲哚美辛,或者20 µM GDC-0449溶解在DMSO中,对照组只含有DMSO。然后加入非荧光的钙黄绿素-AM,最终浓度达到1.0 µM。然后在37oC温育2小时。细胞用含Ca2+,Mg2+的Hank's平衡盐溶液buffer冲洗2次,然后震荡1小时溶解在含0.01% Triton X-100的磷酸缓冲液中,然后放在4oC下过夜。溶解的物质然后转移到96孔板上,钙黄绿素引起的荧光信号用分光光度法定量。所有的实验操作需在暗中进行,所有的读数用平均值±SEM表示。
    (Only for Reference)
动物实验:[4]
- 合并
  • Animal Models: Ptch(+/-)异体移植物模型
  • Dosages: 25 mg/kg,92 mg/kg
  • Administration: 口服
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 84 mg/mL (199.38 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
12.5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 421.3
化学式

C19H14Cl2N2O3S

CAS号 879085-55-9
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03610022 Recruiting Drug: Treatment with vismodegib Metastatic Basal Cell Carcinoma|Locally Advanced Basal Cell Carcinoma University Hospital Bordeaux September 3 2018 Phase 4
NCT03035188 Unknown status Drug: Vismodegib Basal Cell Carcinoma SRH Wald-Klinikum Gera GmbH January 2017 Phase 2
NCT02371967 Completed Drug: Vismodegib Basal Cell Carcinoma Hoffmann-La Roche April 8 2015 --
NCT02667574 Active not recruiting Drug: ERIVEDGE Basal Cell Carcinoma University Hospital Lille|Hoffmann-La Roche November 18 2014 Phase 2
NCT02067104 Terminated Drug: Vismodegib|Drug: Placebo Basal Cell Carcinomas University of Arizona February 17 2014 Phase 2
NCT01774253 Terminated Drug: Vismodegib Pontine Glioma Giselle SaulnierSholler|Phoenix Children''s Hospital|Atrium Health May 2013 Phase 2

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

Hedgehog/Smoothened Signaling Pathway Map

Hedgehog/Smoothened Inhibitors with Unique Features

相关Hedgehog/Smoothened产品

Tags: 购买Vismodegib (GDC-0449) | Vismodegib (GDC-0449)供应商 | 采购Vismodegib (GDC-0449) | Vismodegib (GDC-0449)价格 | Vismodegib (GDC-0449)生产 | 订购Vismodegib (GDC-0449) | Vismodegib (GDC-0449)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID