Smoothened Agonist (SAG) HCl

For research use only. Not for use in humans.

目录号:S7779

Smoothened Agonist (SAG) HCl Chemical Structure

CAS No. 2095432-58-7

Smoothened Agonist (SAG) HCl是一种细胞渗透性Smoothened (Smo)激动剂,其在Shh-LIGHT2细胞中的EC50约为3 nM。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 3660.93 现货
RMB 954.43 现货
RMB 2024.65 现货
RMB 6110.42 现货
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客户使用Selleck生产的Smoothened Agonist (SAG) HCl发表文献38篇:

产品安全说明书

Hedgehog/Smoothened抑制剂选择性比较

生物活性

产品描述 Smoothened Agonist (SAG) HCl是一种细胞渗透性Smoothened (Smo)激动剂,其在Shh-LIGHT2细胞中的EC50约为3 nM。
靶点
Smoothened [1]
(mouse cultured cell assay)
3 nM(EC50)
体外研究

SAG通过直接结合到Smo螺旋束调控Smo活性。[1] SAG以GRK2依赖的方式诱导通过Gli的Smo-依赖性信号。[2] SAG(1 nM)也会诱导神经元和神经胶质前体细胞的增殖,而不影响新生细胞的分化模式。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse Shh-Light II cell MVfGeY5kfGmxbjDhd5NigQ>? MlPZOFghcHK| M1zSPWFkfGm4YYTpc44hd2ZiU3joJJBifGi5YYmgbY4hdW:3c3WgV4hpNUyrZ3j0JGlKKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDncIkyKGW6cILld5Nqd25iYX\0[ZIhPDhiaILzJIJ6KHKnbnnscIEhdHWvaX7ld4NmdmOnIHHzd4F6NCCHQ{WwQVAvODB6ODFOwG0> M3TIXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUi1PVU5Lz5{Mkm4OVk2QDxxYU6=
HUVEC NWDsW443S3m2b4TvfIlkcXS7IHHzd4F6 MnvnNlQhcA>? NGTJeGlEgXSxdH;4bYNqfHliYXfhbY5{fCCKVW\FR{Bi\nSncjCyOEBpenNiYomgW3NVNTFiYYPzZZktKEmFNUC9NlIh|ryP M1\He|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUi1PVU5Lz5{Mkm4OVk2QDxxYU6=
Shh-Light II NWnQO2tmTnWwY4Tpc44h[XO|YYm= M{XqT|Q5KGi{cx?= NFrFTmFC[3SrdnH0bY9vKG:oIGPobEBx[XSqd3H5JIlvKG2xdYPlJHNpcC2OaXfoeEBKUSClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[g[4xqOSCneIDy[ZN{cW:wIHHmeIVzKDR6IHjyd{BjgSC{ZX7pcIxiKGy3bXnu[ZNk\W6lZTDhd5NigSxiRVO1NEA:KDBwME[xPEDPxE1w NIrSNIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mkm4OVk2QCd-MkK5PFU6PTh:L3G+
NIH/3T3 MkPMSpVv[3Srb36gZZN{[Xl? MnzIRYdwdmm|dDDhZ5Rqfmm2eTDheEBUdW9iaX6gcY92e2ViTlnIM|NVOyClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJGdtcS2mZYDlcoRmdnRiZnny[YZtgSCudXPp[oVz[XOnIILldI9zfGW{IHHzd4V{e2WmIHHzJJN1cW23bHH0bY9vKG:oIFfsbUB1emGwc3PybZB1cW:wIH\hZ5RweixiRVO1NEA:KDBwMEOg{txONg>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzR{OUK1OUc,Ojd2MkmyOVU9N2F-
C3H10T1/2 NV;CVJRZTnWwY4Tpc44h[XO|YYm= MlXvOkBl[Xm| Ml70RYdwdmm|dDDhZ5Rqfmm2eTDheEBUdW9iaX6gcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBk\WyuIHTp[oZmemWwdHnheIlwdiCrboTvJI9{fGWxYnzhd5QhcW6ldXLheIVlKG[xcjC2JIRigXNiYomgZYxs[WyrbnWgdIhwe3CqYYThd4Uh[XO|YYmsJGVEPTBiPTCwMlE{KM7:TT6= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzR{OUK1OUc,Ojd2MkmyOVU9N2F-
A673 Mly0dWhVWyCjc4PhfS=> MX\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? NFTJPVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Saos-2 NIK3TXByUFSVIHHzd4F6 M1[0NZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVYX;zMVIh[2WubIO= NX7ZfmJmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-37 NFq3dGtyUFSVIHHzd4F6 NYDhSo85eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz NUj5TYEzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD NHLXb5FyUFSVIHHzd4F6 MWjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-SH NHfjRmFyUFSVIHHzd4F6 NU\xbVhreUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uW0hiY3XscJM> NIXpNYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 NInaTnByUFSVIHHzd4F6 NYTvWGN2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVEzKGOnbHzz M136UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R) MV;xTHRUKGG|c3H5 M{niZ5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucx?= M1fBdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 M3TSdpFJXFNiYYPzZZk> MWjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| M2LzNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 MoG2dWhVWyCjc4PhfS=> MkPZdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= NGrVeY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SJ-GBM2 MYDxTHRUKGG|c3H5 MWnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= NIDjO|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC MoLpdWhVWyCjc4PhfS=> M3rG[5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz Ml:xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 NILn[pJyUFSVIHHzd4F6 MYLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= M2PRdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 MX3xTHRUKGG|c3H5 MUPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? NVXkPXA2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
LC3I / LC3II; 

PubMed: 23504944     


Western blotting for LC3I/II. Huh7, Hep3B and HepG2 cells were incubated with vehicle control, SAG (0.5 µM), Pur (10 µM), Shh (0.4 µg/ml), GANT61 (20 µM) or GDC0449 (20 µM) for 48 hours.

23504944
Immunofluorescence
Smo; 

PubMed: 25636740     


In the absence of SAG, Smo is not detected at the cilium. MRT-92 alone does not induce Smo trafficking to the primary cilium. Cell exposure to SAG induces Smo trafficking to the cilium, which is abolished by MRT-92.

acetylated alpha tubulin; 

PubMed: 25992706     


TM3 cells were cultured in the presence of the SMO agonist (SAG) to monitor response to HH activation. A) In control cells (a-c; 0 h), primary cilia marked by Ac-T (red; arrows) only displayed occasional, speckled appearance of SMO (green). After 1 h in the presence of 100 nM SAG (d-f), a stronger and more frequent localization of SMO to the primary cilia was observed, now also at the ciliary base (f; insert; arrow), and this was even more apparent after 4 h of SAG-stimulation (g-i). Nuceli were stained with DAPI. Scale bar = 10 μm. 

25636740 25992706
体内研究 在成年大鼠海马中,SAG (2.5 nM)脑室内给药显著增加新生细胞的数量,并延长海马细胞的存活时间。[3]在小鼠中,SAG (20 μg/g, i.p.)有效防止GC-诱导的新生小脑发育异常。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:[3]
- 合并
  • Animal Models: 大鼠
  • Dosages: 2.5 nM
  • Administration: 侧脑室给药
    (Only for Reference)

溶解度 (25°C)

体外 Water 100 mg/mL (189.92 mM)
DMSO 71 mg/mL warmed (134.84 mM)
Ethanol 40 mg/mL warmed (75.97 mM)
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
water
100mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 526.52
化学式

C28H29Cl2N3OS

CAS号 2095432-58-7
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03645044 Active not recruiting -- Hiv|HBV Coinfection University of Melbourne|National Health and Medical Research Council Australia|The University of Western Australia|University of Adelaide|The HIV Netherlands Australia Thailand Research Collaboration|University of Malaya|YR Gaitonde Centre for AIDS Research and Education|Melbourne Health May 24 2018 --
NCT03180619 Completed Drug: TAF Chronic Hepatitis B Gilead Sciences June 29 2017 Phase 2
NCT02195518 Completed Drug: Tenofovir disoproxil fumarate Hepatitis B Chronic GlaxoSmithKline March 18 2015 Phase 4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID