Cyclopamine

目录号:S1146 别名: 11-deoxojervine 中文名称:环巴胺

仅限科研使用

Cyclopamine (11-deoxojervine)是一种特异性Hedgehog (Hh)信号通路拮抗剂,作用于Smoothened (Smo),在TM3Hh12细胞中IC50为46 nM。

Cyclopamine Chemical Structure

CAS: 4449-51-8

规格 价格 库存 购买数量
RMB 1227.56 现货
RMB 3833.48 现货
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客户使用Selleck生产的Cyclopamine发表文献110篇:

产品安全说明书

Hedgehog/Smoothened抑制剂选择性比较

相关Hedgehog/Smoothened产品

生物活性

产品描述 Cyclopamine (11-deoxojervine)是一种特异性Hedgehog (Hh)信号通路拮抗剂,作用于Smoothened (Smo),在TM3Hh12细胞中IC50为46 nM。
靶点
Smoothened [1]
(TM3Hh12 cells)
46 nM
体外研究

Cyclopamine 抑制Hedgehog信号通路, IC50 为46 nM, 且在[3H]Hh-Ag结合实验中,作用于CHO-K1细胞,抑制人类Smo受体活性,IC50为 280 nM。[1] Cyclopamine作用于表达Patched (PTCH) mRNA的肠源性肿瘤细胞,显著抑制 Hedgehog通路活性,这种作用存在剂量依赖性,且浓度为3 μM时,抑制肿瘤细胞生长,抑制达75-95%, 但是作用于不表达PTCH mRNA的结肠癌细胞没有效果,说明Cyclopamine 特定作用于Hedgehog 通路,且没有毒性。[2]通过与Smo直接作用而抑制Hedgehog信号,Cyclopamine (10 μM) 抑制SMOhigh Cyclopamine-反应细胞系L3.6sl和 Panc 05.04增殖,抑制达 75-80%,且使凋亡率提高2.5到 3.5倍,但是不影响BxPC3-SMOlow 细胞系。[3] Cyclopamine 处理E3LZ10.7细胞系,显著降低Snail mRNA,且提供E-钙粘着蛋白转录。Cyclopamine处理Hedgehog依赖型 L3.6pl 细胞,显著抑制浸润型细胞, 使转移细胞降低500多倍,但是对Hedgehog非依赖性细胞系Panc-1没有影响。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
OS-RC-2 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnSUYp6UUN3ME21Mlg3PjZizszN MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
DOHH-2 NHTLbVVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH[xb2NKSzVyPUmuN|U3QDlizszN NELMepY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
no-10 MoDhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrFSJNKSzVyPUmuPVA{QSEQvF2= M{S0dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LS-513 NFvyfIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXT4Oow6UUN3ME2xNU4{PTR5IN88US=> NHTXcYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ALL-PO NFnLXJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1:1dmlEPTB;MUGuO|c{PCEQvF2= Ml:1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
8-MG-BA M2rBOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHW1cWNKSzVyPUGzMlEyOjNizszN MojiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
RPMI-8402 MlPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTwSHpKSzVyPUG1Mlg2OzdizszN NVT6UGZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
EoL-1-cell MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjZendKSzVyPUG4MlU6PDhizszN NYjhVFlWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NALM-6 NIm0S2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPyemFmUUN3ME2xPU4xOTZ5IN88US=> NFOyfo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
DEL MmPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jUNmlEPTB;MkCuNVQ4OSEQvF2= MoPhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SR NEnB[I5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlv2TWM2OD1{Mz62O|E2KM7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
697 M1LIZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzlSVFwUUN3ME2yOk43OTV3IN88US=> NH[5Rm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
COLO-829 NVH0cY5xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\uRmlEPTB;Mk[uPFQ5OyEQvF2= M{[xTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
EVSA-T M{nSO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLYfHQxUUN3ME2yO{42PTZzIN88US=> NWL1e3VrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ATN-1 M124bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTNzLkKzNlkh|ryP MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
L-363 NYL3[3pZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDmTWM2OD1|MT63OFYyKM7:TR?= M1HEVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LAMA-84 NVLz[mVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrSTWM2OD1|Mj61NlEyKM7:TR?= M2LFZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NOS-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M320dWlEPTB;M{SuNlk2PiEQvF2= NXHsUXVtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
BB30-HNC MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmSzTWM2OD1|ND6zN|A3KM7:TR?= NGLXfmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
BC-1 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XkdGlEPTB;M{euPVc1PiEQvF2= MlTqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
IST-SL2 MonkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3lU|NyUUN3ME2zPE4zOjRizszN NHPyWpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
D-392MG NYnJS3lqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PBN2lEPTB;NECuNlIyPSEQvF2= MnTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
no-11 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjT[oZnUUN3ME20NE42PTJzIN88US=> M2jZSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LC4-1 NEfnbHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTRyLki3NVYh|ryP MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
A388 MoLMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3qwRWlEPTB;NEKuOVg1QCEQvF2= NXn5XFA{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NTERA-S-cl-D1 Mlr6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LrPGlEPTB;NEKuO|A4PCEQvF2= Mk\RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
CESS MojYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;PNYFKSzVyPUS0MlIzOzJizszN NX3zfVlSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
RS4-11 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXuTWM2OD12OT6wPVM5KM7:TR?= Moq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MS-1 NYXlZm9TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoOwTWM2OD13MD65N|UyKM7:TR?= MkPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
CTV-1 Mn3YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rNV2lEPTB;NUGuNFc1KM7:TR?= MmjRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-502MG MoPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1LXN2lEPTB;NUGuOlI4OSEQvF2= MonpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ML-2 NEXzZZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkPjTWM2OD13Mj65NVk2KM7:TR?= MlrvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SK-NEP-1 NWCxPJV[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTQW49YUUN3ME21N{4{QTJ|IN88US=> M3ziSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LOXIMVI NFn4S2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\HPYJvUUN3ME21N{42QDh2IN88US=> NXPHXYhwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
DJM-1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfWPYxKSzVyPUW2MlM{QTFizszN MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
GI-1 NHPaT2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjaUFlrUUN3ME21Ok43OTR7IN88US=> M17FOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
IST-MES1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;zTWM2OD14MD61OFk{KM7:TR?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MV-4-11 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlOzTWM2OD14MD62OVM5KM7:TR?= NX\BPGtWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
OVCAR-4 NVHnSG8{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7tepZXUUN3ME22N{42PjV5IN88US=> MnrUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KE-37 NHXzR2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVHJR|UxRTZ4LkK2Olgh|ryP NH3zV5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
D-542MG NHrDb3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\hTWM2OD14OD60NVM2KM7:TR?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MHH-PREB-1 MnHDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHReIhLUUN3ME23Nk45PDRzIN88US=> NWm0e4FuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
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MC-CAR NEG2VGJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTtTWM2OD1{MEKuNlU{KM7:TR?= MkfzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SNB75 M1PjWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWfJR|UxRTJ{MT65OEDPxE1? M4DrWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ES4 M{\Lb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3uTWM2OD1{MkOuO|g{KM7:TR?= NEHGcGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KARPAS-422 NF;3RVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXW[5VKSzVyPUKyPE4{PTJizszN NFrqeII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NCI-H1648 NUTtNXFHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTNTWM2OD1{MkmuOFg6KM7:TR?= M2nMW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ES6 M3TxNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnuyTWM2OD1{M{muOFMh|ryP NI\ye249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KNS-81-FD Ml70S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nZdWlEPTB;MkSxMlE6PyEQvF2= NFHDeHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
JAR NYHjZlVnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2i2fmlEPTB;MkW2MlIzPSEQvF2= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NB1 NIi2Z4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHVTFJOUUN3ME2yOlAvPTF4IN88US=> MnL0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-336MG NW[5O29iT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2XqeWlEPTB;Mk[wMlY6QCEQvF2= Mo\tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
BC-3 NHeyZYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTJ4NT6xO|gh|ryP M4XKV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HCC2218 M3HMeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTJ4Nj60NVUh|ryP Mn3zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
TE-9 NX\2dWg3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4faSGlEPTB;Mk[2MlYzPyEQvF2= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LB1047-RCC NEHxdI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTJ4Nj63OVMh|ryP MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
CTB-1 M4T4[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7LTWM2OD1{NkmuPVc{KM7:TR?= NF;hc5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NB7 NV\nZYp[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX[wXmNCUUN3ME2yO|Eh|ryP MlKzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ST486 NHO5eIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTVTWM2OD1{N{euOFEzKM7:TR?= NGDFNXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
HCC1187 NInu[VFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rjOGlEPTB;MkiyMlgyOSEQvF2= M3jKVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NCI-SNU-16 NWDWPJMyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGn3eYVKSzVyPUK4OE4zPDhizszN NFHEfIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
COR-L279 MmjoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\GTWM2OD1{OUGuOVg1KM7:TR?= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ES8 M{PnVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWLJR|UxRTJ7ND6xPFIh|ryP NUHH[I9XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
U-698-M M{LQ[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTJ7OD6yOFMh|ryP M{XFO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HEL M4rE[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXf5XYVHUUN3ME2zNFkvOTR7IN88US=> NIr0co89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KINGS-1 M4jZXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfJR|UxRTNzMD62O|Qh|ryP MlqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KY821 NE\zNodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjOVYRKSzVyPUOzOk42QTVizszN Mm\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MZ1-PC M4[5bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUewT|BIUUN3ME2zOFUvPjF6IN88US=> M4[z[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LS-411N NY\oUYY5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTN3ND62OkDPxE1? NYTjPINGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SIG-M5 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWe3dJJtUUN3ME2zOVkvPzh{IN88US=> NFjBdoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
HT MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7pTWM2OD1|NkeuO|EyKM7:TR?= Mm\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HC-1 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;6TWM2OD1|NkeuO|g4KM7:TR?= M{X4dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NCI-H1694 NEG3fVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XtXmlEPTB;M{eyMlk{PCEQvF2= M{\lXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
BB65-RCC MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLMNlJKSzVyPUO3Ok4zPDVizszN MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HAL-01 NHLj[mNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGG4S41KSzVyPUO3PU45OzhizszN MnrkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ARH-77 M3jKTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnTuTWM2OD1|OUSuNFA5KM7:TR?= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MZ7-mel MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIn2PW9KSzVyPUO5O{4zOzNizszN M13EXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SIMA M1\o[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonqTWM2OD12MEOuPVM{KM7:TR?= MnrWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
DG-75 NH;iVpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTRzNT62PVgh|ryP MnXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HUTU-80 NEjQOohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrUSmRKSzVyPUSxPU4yQDVizszN M4PDWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KNS-42 NETKfXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrvNlAxUUN3ME20NlUvQDF3IN88US=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
SH-4 NHzy[lBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfQZ2dKSzVyPUSyO{42PjVizszN NIn3NHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
L-540 M2D6R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mlu4TWM2OD12M{GuNFMyKM7:TR?= MoHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NB10 NHrQU2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnH2TWM2OD12NEGuNlM1KM7:TR?= NEfXO|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ES1 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjQXW17UUN3ME20OVIvPzV|IN88US=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KMOE-2 M1H2Zmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHiXZlKSzVyPUS1Ok44OTFizszN NHrMXVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
MC116 NF;mV5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7Udm5mUUN3ME20OVgvOTF4IN88US=> MlvHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
RCC10RGB NV\pbJk6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjJTWM2OD12NkCuNFA2KM7:TR?= M17BO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
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A549 Ml73RY51cWOjbnPldkBie3OjeR?= MlfKRY51cWOjbnPldkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZpkhVVSVIHHzd4F6NCCLQ{WwJF0hPDlizszNMi=> NHr1TmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEKyNVg4Oid-MUiyNlE5PzJ:L3G+
DU145 M1LSR2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX3BTXZ7PSC3TR?= MnLPPVYhcHK| Moj0S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gSHUyPDViY3XscJMh[XRiNTD1UUBi\nSncjC5OkBpenN? NXLjUJJoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFkyOjVpPkG4NlQ6OTJ3PD;hQi=>
DU145 M2nwRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYLjdZNlOTBidV2= MmLHPVYhcHK| NULBeZZtT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hTFVzNEWgZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhQTZiaILz MmXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEmxNlUoRjF6MkS5NVI2RC:jPh?=
HEK293 MYTGeY5kfGmxbjDhd5NigQ>? NVHmPGVyUW6qaXLpeIlwdiCxZjDi[ZRiKGejbHHjeI9{cWSjc3WgbY4hUEWNMkmzJINmdGy| MorSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2OUS3OlYoRjF5NEm0O|Y3RC:jPh?=
22Rv Mnr2SpVv[3Srb36gZZN{[Xl? NEfRTYxT\WS3Y4Tpc44hd2ZiZYjwdoV{e2mxbjDv[kBRXEOKIH3SUmEhcW5iaIXtZY4hOjKUdjDj[Yxtew>? MlvPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2OUS3OlYoRjF5NEm0O|Y3RC:jPh?=
PANC1 NGjEVWVHfW6ldHnvckBie3OjeR?= M3:wOFAvOiC3TR?= M2TXNVI1KGi{cx?= M3fHWmlvcGmkaYTpc44hd2ZiSHivS2xKOS2vZXTpZZRm\CCSVFPIJI1TVkFiZYjwdoV{e2mxbjDpckBpfW2jbjDQRW5EOSClZXzsd{BifCByLkKgeW0h[W[2ZYKgNlQhcHK|IHL5JHJVNVCFUh?= NWf2XYllRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OVAyPzBpPkKwOFUxOTdyPD;hQi=>
PANC1 NFXOWZRHfW6ldHnvckBie3OjeR?= NHrYZ4IxNjRidV2= NHW3ZWYzPCCqcoO= MljQTY5pcWKrdHnvckBw\iCKaD;HUGkyNW2nZHnheIVlKFCWQ1igcXJPSSCneIDy[ZN{cW:wIHnuJIh2dWGwIGDBUmMyKGOnbHzzJIF1KDBwNDD1UUBi\nSncjCyOEBpenNiYomgVnQuWEOU M4TPWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWwNVcxLz5{MES1NFE4ODxxYU6=
Shh Light2 MlTkSpVv[3Srb36gZZN{[Xl? Ml;COk4zPSC3TR?= MoPmN|AhcHK| MljxTY5pcWKrdHnvckBw\iCQLYDhcI1qfG:7bHH0[YQhW2iqIHnuJI1wfXOnIGPobEBNcWeqdEKgZ4VtdHNiYYSgOk4zPSC3TTDh[pRmeiB|MDDodpMh[nliZnny[YZtgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= M1zvW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUWxO|MyLz5zOUG1NVc{OTxxYU6=
U87MG MVLGeY5kfGmxbjDhd5NigQ>? MV2xNEB2VQ>? NVTVdo5[PCCqcoO= MV;Jcohq[mm2aX;uJI9nKEinZHflbI9oKHOrZ37hcIlv\yCyYYToe4F6KGmwIHj1cYFvKFV6N13HJINmdGy|IHHzd4V{e2WmIHHzJIRwf25icnXneYxifGmxbjDv[kBIdGlzIHH0JFExKHWPIHHmeIVzKDRiaILzJIJ6KFKWLWDDVkBidmGueYPpdy=> MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ{Nk[1O{c,OjJ{Mk[2OVc9N2F-
HEK293 NIjtb3dHfW6ldHnvckBie3OjeR?= NFnIPIE2KHWP NXfjemUyOTBiaILz MVPEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NWO7Y3zvdIFucW6nIH\yc40hcHWvYX6gV41wKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XRiNTD1UUBu\WG|dYLl[EBi\nSncjCxNEBpenNiYomgSGFRUSC|dHHpcolv\yCkYYPl[EBndHWxcnXzZ4Vv[2VibXnjdo9{[2:yaXOgZZN{[Xl? MnWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5M{[wOlMoRjJ5N{O2NFY{RC:jPh?=
HEK293 Ml3OSpVv[3Srb36gZZN{[Xl? MnnaOUB2VQ>? MX2xNEBpenN? M1TK[GRqe3CuYXPlcYVvfCCxZjDCU2RKWFlvY4njcI9x[W2rbnWg[pJwdSCqdX3hckBUdW9iZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjdDC1JJVOKG2nYYP1doVlKGGodHXyJFExKGi{czDifUBHSUOVIHHuZYx6e2m| M3zYUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{O2NFY{Lz5{N{ezOlA3OzxxYU6=
C3H10T1/2 NFPqW2RHfW6ldHnvckBie3OjeR?= NWP6N4VuPTVwNTD0c{A1PTByIH7N NW\MRXRyS2:vcHX0bZRqfmViaX7obYJqfGmxbjDv[kBUdW9iaX6gcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhW0GJLXnu[JVk\WRiR3zpNUB1emGwc3PybZB1cW:wYXygZYN1cX[rdImgZZQhPTVwNTD0c{A1PTByIH7NJIJ6KHGSQ2KgZY5idHm|aYO= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB5NEW0NUc,OjNyN{S1OFE9N2F-
U2OS NGHpZWdHfW6ldHnvckBie3OjeR?= NV3nfoFrPSC3TR?= NHXadpg3KGi{cx?= NHTD[VRCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHPobY1memmlIGPtc{A3OzNibYX0ZY51KGW6cILld5Nm\CCrbjDVNm9UKGOnbHzzJINw\XiycnXzd4lv\yCkZYThJIFzemW|dHnuNk1ITlBiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCrboTyZYNmdGy3bHHyJIJmfGFiYYLy[ZN1cW5{LVfGVEBi\2e{ZXfheIUh\m:{bXH0bY9vKGG2IEWgeW0h[W[2ZYKgOkBpenNiYomgZ49v\m:lYXygcYlkem:|Y3;wfS=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4M{WyNkc,OjNyNkO1NlI9N2F-
Assay
Methods Test Index PMID
Western blot Snail / E-cadherin / Slug / Vimentin Gli1 / TGF-β1 / CXCR4 NF-κB / Cyclin D1 / MMP2 / MMP9 26859575 26137001 23599805
Immunofluorescence PCNA / β-catenin Vimentin 28747625 24553082
体内研究 Cyclopamine 每天按50 mg/kg剂量处理小鼠,持续22天,消除HUCCT1移植瘤,且没有明显副作用。[2] Cyclopamine 按1.2 mg剂量处理Panc 05.04和 L3.6sl驱动的肿瘤7天,前者诱导明显的肿瘤细胞凋亡,后者降低肿瘤质量,降低达50-60%,但是作用于BxPC3-SMOlow肿瘤没有效果。[3] Cyclopamine单独处理E3LZ10.7 和L3.6pl移植瘤,显著抑制肿瘤转移,且和 Gemcitabine联用处理,完全废除转移。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
  • 激酶实验:

    Gli-Luc实验测定Hh 信号通路末期, 即使用荧光素酶进行读数,测定Gli的转录调控。Cyclopamine 在DMSO中连续稀释用于实验,然后加到空的实验板上。TM3Hh12 细胞(TM3 细胞含Hh反应受体基因结构 pTA-8xGli-Luc) 悬浮在含5% FBS 和15 mM Hepes pH 7.3的F12 Ham’s/DMEM (1:1)培养基上, 加到实验板上,与Cyclopamine在37oC下温育30分钟左右。1 nM Hh-Ag 1.5 加到实验板上,然后在37oC下温育。48小时后,在实验板中加入Bright-Glo或MTS,然后在492 nm处测定发光度或吸光度。通过分析反应曲线,使用R统计软件包测定IC50值。

细胞实验:[2]
  • Cell lines: SEG1, OE33, KYAE, KYSE180, SNU1, AGS, SNU16, NCI-N-87, HUCCT1, PANC1, PL5, PL6, BXPC3, HS766T, KYSE150, GBD1, DLD1, 和HCT116
  • Concentrations: 溶于DMSO, 终浓度为3 μM
  • Incubation Time: 4天
  • Method: 在96孔板上,用Cyclopamine 处理细胞。通过MTS实验测定细胞活力。进行CellTiter96比色测定,进行2和4天,然后在490 nm处通过测定光密度而测定存活细胞。
动物实验:[2]
  • Animal Models: 皮下注射HUCCT1细胞的无胸腺裸鼠
  • Dosages: 50 mg/kg/day
  • Administration: 皮下注射处理

溶解度(25°C)

体外

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 411.62
化学式

C27H41NO2

CAS号 4449-51-8
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

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操作手册

如果有其他问题,请给我们留言。

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常见问题及建议解决方法

问题 1:
How to reconstitute the compound for in vivo use in mice?

回答:
One paper dissolved this drug in DMSO, and diluted in saline: Berman DM, et al. Nature, 2003, 425(6960), 846-851. Alternatively, you can try this vehicle: 10% DMSO+30% PEG 300+5% Tween 80+ddH2O for P.O. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG300 and Tween, after they mixed well, dilute with water.

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