Cyclopamine

For research use only. Not for use in humans.

目录号：S1146 别名： 11-deoxojervine 中文名称：环巴胺

CAS No. 4449-51-8

Cyclopamine (11-deoxojervine)是一种特异性Hedgehog (Hh)信号通路拮抗剂，作用于Smoothened (Smo)，在TM3Hh12细胞中IC50为46 nM。

规格

价格

库存

购买数量

5mg	RMB 1227.56	现货
25mg	RMB 3037.37	现货
50mg	RMB 3833.48	现货
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客户使用Selleck生产的Cyclopamine发表文献85篇：

Nature, 2015, 10.1038/nature14325

PubMed: 25938716 ( click the link to review the publication )

Cancers (Basel), 2021, 13(3)418

PubMed: 33499351 ( click the link to review the publication )

J Cell Mol Med, 2021, 10.1111/jcmm.16313

PubMed: 33507617 ( click the link to review the publication )

Oncol Lett, 2021, 21(4):295

PubMed: 33732371 ( click the link to review the publication )

J Orthop Surg Res, 2021, 16(1):160

PubMed: 33637095 ( click the link to review the publication )

Can J Physiol Pharmacol, 2021, 10.1139/cjpp-2020-0588

PubMed: 33617370 ( click the link to review the publication )

Invest Ophthalmol Vis Sci, 2021, 62(2):33

PubMed: 33616621 ( click the link to review the publication )

Nat Commun, 2020, 11(1):44

PubMed: 31896750 ( click the link to review the publication )

Cell Rep, 2020, 21;31(3):107521

PubMed: 32320667 ( click the link to review the publication )

Int J Cancer, 2020, 7

PubMed: 32383186 ( click the link to review the publication )

Stem Cell Reports, 2020, S2213-6711(20)30192-2

PubMed: 32589876 ( click the link to review the publication )

J Clin Endocrinol Metab, 2020, dgaa656

PubMed: 32936899 ( click the link to review the publication )

Stem Cell Res Ther, 2020, 11(1):488

PubMed: 33198821 ( click the link to review the publication )

Oxid Med Cell Longev, 2020, 20;2020:1868764

PubMed: 32377291 ( click the link to review the publication )

Proc Biol Sci, 2020, 287(1935):20192939

PubMed: 32933441 ( click the link to review the publication )

Chemosphere, 2020, 6;254:126709

PubMed: 32348926 ( click the link to review the publication )

Front Cell Neurosci, 2020, 14:575690

PubMed: 33343302 ( click the link to review the publication )

Int J Mol Sci, 2020, 21(17)E6410

PubMed: 32899202 ( click the link to review the publication )

Acta Pharmacol Sin, 2020, 10.1038/s41401-020-00504-4

PubMed: 32855528 ( click the link to review the publication )

Dev Biol, 2020, 457(1):128-139

PubMed: 31550483 ( click the link to review the publication )

Cancer Med, 2020, 10.1002/cam4.2873

PubMed: 32030915 ( click the link to review the publication )

Oncol Rep, 2020, 44(6):2610-2620

PubMed: 33125122 ( click the link to review the publication )

Front Pharmacol, 2020, 11:204

PubMed: 32194421 ( click the link to review the publication )

Neural Plast, 2020, 2020:8815195

PubMed: 32802036 ( click the link to review the publication )
Dev Cell, 2019, 51(6):665-674.e6

PubMed: 31813796 ( click the link to review the publication )

J Cell Biol, 2019, 218(9):3134-3152

PubMed: 31371388 ( click the link to review the publication )

Elife, 2019, 8e50208

PubMed: 31482846 ( click the link to review the publication )

Cancers (Basel), 2019, 11(10)

PubMed: 31658643 ( click the link to review the publication )

Hum Mol Genet, 2019, 28(6):896-911

PubMed: 30445456 ( click the link to review the publication )

J Cell Physiol, 2019, 234(3):2058-2066

PubMed: 30317648 ( click the link to review the publication )

Int J Mol Sci, 2019, 20(8)E1991

PubMed: 31018557 ( click the link to review the publication )

J Cell Biochem, 2019, 120(4):5386-5395

PubMed: 30367510 ( click the link to review the publication )

J Cell Biochem, 2019, 120(11):18979-18994

PubMed: 31245876 ( click the link to review the publication )

Exp Cell Res, 2019, 385(2):111671

PubMed: 31634481 ( click the link to review the publication )

Basic Clin Pharmacol Toxicol, 2019, 124(6):660-669

PubMed: 30548093 ( click the link to review the publication )

Hepatobiliary Pancreat Dis Int, 2019, 18(1):38-47

PubMed: 30243878 ( click the link to review the publication )

Phytomedicine, 2019, 67:153163

PubMed: 31901891 ( click the link to review the publication )

Neuron, 2018, 97(6):1235-1243

PubMed: 29526553 ( click the link to review the publication )

EMBO Rep, 2018, 19(2):269-289

PubMed: 29263200 ( click the link to review the publication )

Elife, 2018, 7

PubMed: 30014845 ( click the link to review the publication )

Lab Chip, 2018, 18(20):3184-3195

PubMed: 30204194 ( click the link to review the publication )

Exp Cell Res, 2018, 366(1):63-70

PubMed: 29548749 ( click the link to review the publication )

Mol Pain, 2018, 14:1744806918767560

PubMed: 29607715 ( click the link to review the publication )

Biol Reprod, 2018, 98(4):480-490

PubMed: 29329377 ( click the link to review the publication )

Oncol Rep, 2018, 39(1):21-30

PubMed: 29115520 ( click the link to review the publication )

Cell Transplant, 2018, 27(3):393-406

PubMed: 29749250 ( click the link to review the publication )

Anticancer Drugs, 2018, 29(10):995-1003

PubMed: 30106753 ( click the link to review the publication )

Int J Clin Exp Pathol, 2018, 11(5):2920-2930

PubMed: 31938417 ( click the link to review the publication )
Nat Commun, 2017, 8:14382

PubMed: 28176763 ( click the link to review the publication )

PLoS Genet, 2017, 13(8):e1006951

PubMed: 28797033 ( click the link to review the publication )

Front Immunol, 2017, 9:2847

PubMed: 30568656 ( click the link to review the publication )

Cancer Discov, 2017,

PubMed: 28923910 ( click the link to review the publication )

Cell Physiol Biochem, 2017, 43(5):1813-1828

PubMed: 29065414 ( click the link to review the publication )

J Cell Mol Med, 2017, 21(3):568-578

PubMed: 27709831 ( click the link to review the publication )

Exp Cell Res, 2017, 357(1):79-87

PubMed: 28461158 ( click the link to review the publication )

Int J Biochem Cell Biol, 2017, 89:147-156

PubMed: 28624529 ( click the link to review the publication )

Mol Immunol, 2017, 92:68-75

PubMed: 29054053 ( click the link to review the publication )

Onco Targets Ther, 2017, 10:5605-5619

PubMed: 29200875 ( click the link to review the publication )

Oncotarget, 2017, 8(26):42495-42509

PubMed: 28477008 ( click the link to review the publication )

EMBO Rep, 2016, 17(5):682-94

PubMed: 27113760 ( click the link to review the publication )

J Med Chem, 2016, 59(24):11050-11068

PubMed: 27736063 ( click the link to review the publication )

Cell Biosci, 2016, 10.1186/s13578-016-0104-8

PubMed: 27313840 ( click the link to review the publication )

Oncotarget, 2016, 7(6):6626-38

PubMed: 26675547 ( click the link to review the publication )

J Hematol Oncol, 2015, 8:63

PubMed: 26048374 ( click the link to review the publication )

Development, 2015, 142(15):2610-22

PubMed: 26116667 ( click the link to review the publication )

Am J Cancer Res, 2015, 5(9):2569-89

PubMed: 26609469 ( click the link to review the publication )

Mol Carcinog, 2015, 10.1002/mc.22300

PubMed: 25728352 ( click the link to review the publication )

Int J Mol Med, 2015, 35(6):1641-50

PubMed: 25872645 ( click the link to review the publication )

Stem Cell Reports, 2015, 5(5):816-828

PubMed: 26527387 ( click the link to review the publication )

Cancer Biol Ther, 2014, 15(5):544-59

PubMed: 24553082 ( click the link to review the publication )

Eur J Pharmacol, 2014, 10.1016/j.ejphar.2014.01.074

PubMed: 24534491 ( click the link to review the publication )

Mol Cell Biochem, 2014, 396(1-2):257-68

PubMed: 25064451 ( click the link to review the publication )
Biochem Biophys Res Commun, 2014, 10.1016/j.bbadis.2014.10.001

PubMed: 25305367 ( click the link to review the publication )

Insect Sci, 2014, 10.1111/1744-7917

PubMed: 25154865 ( click the link to review the publication )

Dev Biol, 2013, 381(2):312-23

PubMed: 23872235 ( click the link to review the publication )

Dev Cell, 2013, 25(2):169-81

PubMed: 23602386 ( click the link to review the publication )

J Exp Clin Cancer Res, 2013, 5;32(1):7

PubMed: 23379358 ( click the link to review the publication )

Toxicol Lett, 2013, 225(2):318-324

PubMed: 24388991 ( click the link to review the publication )

PLoS One, 2013, 8(3):e57346

PubMed: 23483902 ( click the link to review the publication )

Oncotarget, 2013, 4(10):1698-1711

PubMed: 24091605 ( click the link to review the publication )

Liver Int, 2013, 33(1):149-61

PubMed: 23121638 ( click the link to review the publication )

Neuro Oncol, 2012, 14(12):1441-51

PubMed: 23104476 ( click the link to review the publication )

Cancer Res, 2012, 72(9):2262-74

PubMed: 22396494 ( click the link to review the publication )

Cancer Lett, 2012, 322(2):169-76

PubMed: 22450749 ( click the link to review the publication )

Exp Hematol, 2012, 40(5):418-27

PubMed: 22240607 ( click the link to review the publication )

产品安全说明书

Hedgehog/Smoothened抑制剂选择性比较

生物活性

产品描述

Cyclopamine (11-deoxojervine)是一种特异性Hedgehog (Hh)信号通路拮抗剂，作用于Smoothened (Smo)，在TM3Hh12细胞中IC50为46 nM。

靶点

Smoothened ^[1]
(TM3Hh12 cells)

46 nM

体外研究

Cyclopamine 抑制Hedgehog信号通路， IC50 为46 nM, 且在[3H]Hh-Ag结合实验中，作用于CHO-K1细胞，抑制人类Smo受体活性，IC50为 280 nM。^[1] Cyclopamine作用于表达Patched (PTCH) mRNA的肠源性肿瘤细胞，显著抑制 Hedgehog通路活性，这种作用存在剂量依赖性，且浓度为3 μM时，抑制肿瘤细胞生长，抑制达75-95%, 但是作用于不表达PTCH mRNA的结肠癌细胞没有效果，说明Cyclopamine 特定作用于Hedgehog 通路，且没有毒性。^[2]通过与Smo直接作用而抑制Hedgehog信号，Cyclopamine (10 μM) 抑制SMOhigh Cyclopamine-反应细胞系L3.6sl和 Panc 05.04增殖，抑制达 75-80%,且使凋亡率提高2.5到 3.5倍，但是不影响BxPC3-SMOlow 细胞系。^[3] Cyclopamine 处理E3LZ10.7细胞系，显著降低Snail mRNA，且提供E-钙粘着蛋白转录。Cyclopamine处理Hedgehog依赖型 L3.6pl 细胞，显著抑制浸润型细胞, 使转移细胞降低500多倍，但是对Hedgehog非依赖性细胞系Panc-1没有影响。^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
OS-RC-2	NY\2XY97T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXfJR\|UxRTVwOE[2OkDPxE1?	M2rGPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
DOHH-2	M4iwXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MX;JR\|UxRTlwM{W2PFkh\|ryP	M3TRXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
no-10	M2PG[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NGn4SlFKSzVyPUmuPVA{QSEQvF2=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LS-513	NIf3c3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NEO0bFVKSzVyPUGxMlM2PDdizszN	NHz5[ok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
ALL-PO	NFKyVVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MXrJR\|UxRTFzLke3N\|Qh\|ryP	NXfmeHpjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
8-MG-BA	MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MkLPTWM2OD1zMz6xNVI{KM7:TR?=	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
RPMI-8402	NITORlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NEPmT3lKSzVyPUG1Mlg2OzdizszN	M{TuU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
EoL-1-cell	MliwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NVfYNYxWUUN3ME2xPE42QTR6IN88US=>	NU\1ZoFURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NALM-6	MkHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NGLhVHFKSzVyPUG5MlAyPjdizszN	NGPZUI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
DEL	NUnvZ2p{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NHrwfWVKSzVyPUKwMlE1PzFizszN	MlntQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SR	Mn7xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MV7JR\|UxRTJ\|Lk[3NVUh\|ryP	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
697	NVr6eIhbT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXHJR\|UxRTJ4Lk[xOVUh\|ryP	NVLvW4hVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
COLO-829	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MXHJR\|UxRTJ4Lki0PFMh\|ryP	NHjwcIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
EVSA-T	M{Ttcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			Ml:wTWM2OD1{Nz61OVYyKM7:TR?=	M4HiO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ATN-1	NVXY[2RiT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NVvk[2t{UUN3ME2zNU4zOzJ7IN88US=>	NX;ueHR1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
L-363	NHHyWnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NIXie4pKSzVyPUOxMlc1PjFizszN	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LAMA-84	NG\DXmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVLJR\|UxRTN{LkWyNVEh\|ryP	NIjxenc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
NOS-1	MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NInKZ5RKSzVyPUO0MlI6PTZizszN	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BB30-HNC	Ml23S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NX70TpRyUUN3ME2zOE4{OzB4IN88US=>	NHrkVWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
BC-1	NF\jW4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MkDOTWM2OD1\|Nz65O\|Q3KM7:TR?=	M3zlUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
IST-SL2	MlHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MonXTWM2OD1\|OD6yNlQh\|ryP	M3O1W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
D-392MG	M1fMNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M3:wfmlEPTB;NECuNlIyPSEQvF2=	NYHNdIVVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
no-11	NIDUfJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MmnsTWM2OD12MD61OVIyKM7:TR?=	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LC4-1	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NHe2[4RKSzVyPUSwMlg4OTZizszN	NF;WbpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
A388	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NIPLfYtKSzVyPUSyMlU5PDhizszN	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NTERA-S-cl-D1	M{\xc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NWDZRmNXUUN3ME20Nk44ODd2IN88US=>	NUnYO5Q5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
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LB647-SCLC	MmXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NXnyUXB[UUN3ME2xO\|UvQDR3IN88US=>	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
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NOMO-1	NXLNVldkT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NX7WfG9oUUN3ME2xPFIvQDVizszN	NVjQS3dqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
GI-ME-N	M1exXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NHPie4ZKSzVyPUG4O{46PjlizszN	MljrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KMS-12-PE	NXvHcGs6T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MlLNTWM2OD1zOEmuNlc{KM7:TR?=	MkD2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
Daudi	M3vwb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MWfJR\|UxRTF7MT6xNlgh\|ryP	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LB996-RCC	MnrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M4HPSGlEPTB;MUmxMlY6QSEQvF2=	Mn\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NCI-H2107	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			Mo\3TWM2OD1zOUOuO\|M6KM7:TR?=	M1LmO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
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MC-CAR	NHPzSYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MkDwTWM2OD1{MEKuNlU{KM7:TR?=	NELKUJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
SNB75	M3;ZSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M3PpeWlEPTB;MkKxMlk1KM7:TR?=	Mn2zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ES4	MnHQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M1G2Z2lEPTB;MkKzMlc5OyEQvF2=	NWrlZoQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KARPAS-422	MkDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M2XMWmlEPTB;MkK4MlM2OiEQvF2=	MnW5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NCI-H1648	M4fFRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NFOwfIJKSzVyPUKyPU41QDlizszN	MoL0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ES6	MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MVXJR\|UxRTJ\|OT60N{DPxE1?	NVnCSVg4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KNS-81-FD	M3PCNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NYjmWHJtUUN3ME2yOFEvOTl5IN88US=>	NV\UUY1KRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
JAR	NV23OlA2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MVHJR\|UxRTJ3Nj6yNlUh\|ryP	M2nhZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
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D-336MG	M1TKNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NV7pUWI5UUN3ME2yOlAvPjl6IN88US=>	M3\O[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
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HCC2218	M4nEUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M3;kRWlEPTB;Mk[2MlQyPSEQvF2=	MnPrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
TE-9	MnfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M4rzcWlEPTB;Mk[2MlYzPyEQvF2=	M37sOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LB1047-RCC	M{e0fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M1G0[mlEPTB;Mk[2Mlc2OyEQvF2=	NInCVWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
CTB-1	NXLt[odMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MkDyTWM2OD1{NkmuPVc{KM7:TR?=	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
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HCC1187	MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M3HmSGlEPTB;MkiyMlgyOSEQvF2=	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-SNU-16	M17kU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MlTSTWM2OD1{OESuNlQ5KM7:TR?=	NGfxW3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
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HEL	NFy2WmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NYPsTplFUUN3ME2zNFkvOTR7IN88US=>	NHP5[249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
KINGS-1	NHS5eWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVvJR\|UxRTNzMD62O\|Qh\|ryP	MkPZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KY821	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M{\Ld2lEPTB;M{O2MlU6PSEQvF2=	Ml3LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MZ1-PC	NV3JNG1bT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Ml\kTWM2OD1\|NEWuOlE5KM7:TR?=	NEnXSo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
LS-411N	M4TYXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M37p[GlEPTB;M{W0MlY3KM7:TR?=	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
SIG-M5	NFrlWWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVXJR\|UxRTN3OT63PFIh\|ryP	M4TvSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HT	Ml60S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NHPtN\|lKSzVyPUO2O{44OTFizszN	M3yzV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HC-1	NHzNPYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MUfJR\|UxRTN4Nz63PFch\|ryP	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H1694	M4j4cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M2DTeGlEPTB;M{eyMlk{PCEQvF2=	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BB65-RCC	NVj6c\|Q5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M4LacWlEPTB;M{e2MlI1PSEQvF2=	M2fGeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HAL-01	NFm4NHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NFnNeYFKSzVyPUO3PU45OzhizszN	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ARH-77	MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M2\QeGlEPTB;M{m0MlAxQCEQvF2=	NXnBR4FiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MZ7-mel	MlfjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MWDJR\|UxRTN7Nz6yN\|Mh\|ryP	NULoNmRMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SIMA	NFrYZm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MmnBTWM2OD12MEOuPVM{KM7:TR?=	MmjuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
DG-75	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M3XQO2lEPTB;NEG1MlY6QCEQvF2=	Ml3MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HUTU-80	MmTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NGr1VnJKSzVyPUSxPU4yQDVizszN	NWDrN2ZvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KNS-42	MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NGDPSGVKSzVyPUSyOU45OTVizszN	NWPY[oFCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SH-4	MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			Ml\tTWM2OD12MkeuOVY2KM7:TR?=	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
L-540	M3u2TGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NVHFeYFTUUN3ME20N\|EvODNzIN88US=>	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NB10	MmWyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MVfJR\|UxRTR2MT6yN\|Qh\|ryP	NVLWZ216RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ES1	Mn7qS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MkXzTWM2OD12NUKuO\|U{KM7:TR?=	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KMOE-2	NWP5TIFpT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MnLLTWM2OD12NU[uO\|EyKM7:TR?=	NHjPfZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK\|5USU6JRWK8M4E,
MC116	NIjiXGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NVPpe3lLUUN3ME20OVgvOTF4IN88US=>	Mn6yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
RCC10RGB	M4C2NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			Ml:zTWM2OD12NkCuNFA2KM7:TR?=	NW\xXpplRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
RL95-2	M3u0RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			Mn;3TWM2OD12NkCuNlM4KM7:TR?=	NY\pTmdrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
Raji	NH:5N5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M3jtdGlEPTB;NE[4MlE1OyEQvF2=	NXz3UHd6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
CAS-1	NE\uWI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M13zO2lEPTB;NEeyMlA4OyEQvF2=	M4rndlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
Calu-6	M3[4Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NF;oSHJKSzVyPUS3OU4zPjVizszN	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KG-1	NYD0enRGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NHvRVHFKSzVyPUS3PE41PCEQvF2=	NYfVTmNHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LB771-HNC	MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MWDJR\|UxRTR6Mj6yN\|Ih\|ryP	M4rmXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ACN	MnTMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M1v0WWlEPTB;NEmzMlU6QSEQvF2=	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KM12	NH;jWGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MkXMTWM2OD12OU[uOVg6KM7:TR?=	Ml;tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
U2OS	NIniXFBHfW6ldHnvckBie3OjeR?=			M3PmcmJqdmSrbnegZYZncW6rdImgeI8hf2muZDD0fZBmKFOvbzDlfJBz\XO\|ZXSgbY4hXTKRUzDj[YxteyCkeTDzZ4lvfGmubHH0bY9vKGOxdX70bY5oNCCNZDC9JFAvODF{NDFOwG0v	M2PBWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|ME[zOVIzLz5{M{C2N\|UzOjxxYU6=
U2OS	MWHGeY5kfGmxbjDhd5NigQ>?		NVfPUoRVOiCqcoO=	NUPXNHZzTGm\|cHzhZ4Vu\W62IH;mJHs{UF2leXPsc5BidWmwZTDmdo9uKHerbHSgeJlx\SCVbX:g[ZhxemW\|c3XkJIlvKFV{T2OgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4khRSByLkCxNlch\|ryPLh?=	Moq0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ\|ME[zOVIzRC:jPh?=
TM3	MUXGeY5kfGmxbjDhd5NigQ>?		NH\iV2w1QCCqcoO=	MkPMTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC\|aXfuZYxqdmdicHH0bJdigSCrbjDtc5V{\SCWTUOgZ4VtdHNiYnXhdolv\yCyVFGtPJhIdGlvTIXjJJJmeG:{dHXyJINwdnO2coXjeEBie3Onc4Pl[EBieyC2cnHud4NzcXC2aX;uZYwhdW:mdXzheIlwdiCxZjDHcIkh[W[2ZYKgOFghcHK\|IHL5JIx2[2moZYLhd4Uh[XO\|YYmsJGlEPTBiPTCwMlA1PiEQvF2u	M2fXXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEmxOVU6Lz5zOUC5NVU2QTxxYU6=
HCC827	NE\oXFlHfW6ldHnvckBie3OjeR?=			NGnQToNFcXOybHHj[Y1mdnRib3[gX\|NJZS2leXPsc5BidWmwZTDmdo9uKFOPTzDWOFA1VSCvdYThcpQhcW5iZ3XmbZRqdmmkIILld4l{fGGwdDDoeY1idiCKQ1O4Nlch[2WubIOgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4khRSByLkC1NUDPxE1w	NGPwUVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEe4O\|E2Pid-Mki3PFcyPTZ:L3G+
HEK293	MW\GeY5kfGmxbjDhd5NigQ>?		NHTYSmwzKGi{cx?=	NGf3[oZFcXOybHHj[Y1mdnRib3[gRm9FUVC\LXzhZoVtdGWmIHP5Z4xweGGvaX7lJIZzd21iaIXtZY4hW22xIILlZ4VxfG:{IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYX\0[ZIhOiCqcoOgZpkh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcImsJGlEPTBiPTCwMlA3PCEQvF2u	NIi2ZVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2PFU2OSd-MkKyOlg2PTF:L3G+
Shh Light2	NX3xcZZ[TnWwY4Tpc44h[XO\|YYm=		MoX6N\|AhcHK\|	NGO0[HVKdmirYnn0bY9vKG:oIHjl[IdmcG:pIIPp[45idGmwZzDwZZRpf2G7IHnuJI1wfXOnIGPobEBNcWeqdEKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC\|b37pZ{Bp\WSpZXjv[{1qdmS3Y3XkJGdNUTFvbXXkbYF1\WRidILhcpNkemmydHnvcoFtKGGldHn2bZR6KG2nYYP1doVlKGGodHXyJFMxKGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvODd2MTFOwG0v	MlywQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd3NkezO\|EoRjJ5NU[3N\|cyRC:jPh?=
U2OS	NV;LWWFGTnWwY4Tpc44h[XO\|YYm=			NED5SG5DcW6maX7nJIFn\mmwaYT5JJRwKFOvbzDEOFc{UCCvdYThcpQh\XiycnXzd4VlKGmwIGWyU3Mh[2WubIOgZpkhe2OrboTpcIxifGmxbjDjc5VvfGmwZzygT4QhRSByLkGxOkDPxE1w	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4M{WyNkc,OjNyNkO1NlI9N2F-
DaOY	M3;NZWZ2dmO2aX;uJIF{e2G7		NX\0N\|ZyPDhiaILz	MYLJcohq[mm2aX;uJI9nKEinZHflbI9oKHOrZ37hcIlv\yCrbjDoeY1idiCGYV;ZJINmdGy\|IHHzd4V{e2WmIHHzJIRwf26{ZXf1cIF1cW:wIH;mJGdtcTFibWLORUBmgHC{ZYPzbY9vKGGodHXyJFQ5KGi{czDifUBTXC2SQ2KgZY5idHm\|aYOsJGlEPTBiPTCwMlE3KM7:TT6=	NHzJ[lU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFcyPid-MkS5NFA4OTZ:L3G+
U2OS	MkLRSpVv[3Srb36gZZN{[Xl?		M2fQRVIhcHK\|	NYXaPWhzTGm\|cHzhZ4Vu\W62IH;mJHs{UF2leXPsc5BidWmwZTDmdo9uKFOvbzDEOFc{UCCvdYThcpQh\XiycnXzd4VlKGmwIGWyU3Mh[2WubIOgZYZ1\XJiMjDodpMh[nlic3PpcpRqdGyjdHnvckBkd3WwdHnu[{whU2liPTCwMlI{OiEQvF2u	MmfqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ\|ME[zOVIzRC:jPh?=
CHO	NGDYPZFHfW6ldHnvckBie3OjeR?=			NXPHRnpvSW62YXfvcol{fCCjY4Tpeol1gSCjdDDoeY1idiCVbX:gdoVk\XC2b4Kg[ZhxemW\|c3XkJIlvKEOKTzDj[YxteyCkeTDbN2heUGhvQXegZolv\GmwZzDhd5NigSxiSVO1NEA:KDBwMkig{txONg>?	Mmf0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyOUG1OVkoRjF7MEmxOVU6RC:jPh?=
Shh Light2	NInCfldHfW6ldHnvckBie3OjeR?=		NWTabJljPDBiaILz	M{THdWlvcGmkaYTpc44hd2ZiU1jIJJBifGi5YYmgbY4hdW:3c3WgV4hpKEyrZ3j0NkBk\WyuczDh[pRmeiB2MDDodpMh[nliR3zpMYRmeGWwZHXueEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVAhRSByLkOg{txONg>?	MnXiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3OUK3PFgoRjJzNUmyO\|g5RC:jPh?=
Shh-light2	MV;GeY5kfGmxbjDhd5NigQ>?			M1jnRmlvcGmkaYTpc44hd2ZiU33vMY1m\GmjdHXkJGhpKHOrZ37hcIlv\yCrbjDoeY1idiCVaHitcIlocHR{IHPlcIx{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3MDC9JFAvOyEQvF2u	NFrDbpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2PFU2OSd-MkKyOlg2PTF:L3G+
M210B4	MnroSpVv[3Srb36gZZN{[Xl?		MWOyOEBpenN?	M1PQcWlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIHnuJI1wfXOnIF2yNVBDPCClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCSdHPoJI1TVkFiZYjwdoV{e2mxbjDh[pRmeiB{NDDodpMh[nliUmStVGNTKGGwYXz5d4l{NCCLQ{WwJF0hOC52MzFOwG0v	NV\MOXI5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA4OTZpPkK0PVAxPzF4PD;hQi=>
Shh-Light 2	NWnOWJRDTnWwY4Tpc44h[XO\|YYm=		MXeyJIRigXN?	NWLVbplVSW62YXfvcol{fCCjY4Tpeol1gSCjdDDTcY8hcW5ibX;1d4UhW2iqLVzp[4h1KDJiY3XscJMh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBUcGhvaX7keYNm\CCJbHmxMZJmeG:{dHXyJIFkfGm4aYT5JIFnfGW{IEKg[IF6eyCkeTDkeYFtNWy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBu\XSqb3SsJGlEPTBiPTCwMlQ5PCEQvF2u	M3exflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|ME[zOVIzLz5{M{C2N\|UzOjxxYU6=
Shh Light2	MVvGeY5kfGmxbjDhd5NigQ>?			NIj1bJBKdmirYnn0bY9vKG:oIGPITEBqdiCvb4Xz[UBUcGhiTHnnbJQzKGOnbHzzJIJ6KEeOST3y[ZNxd26\|aY\lJIZqemWobImgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC53IN88UU4>	NGjUSno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUOwPVA5OCd-MUmzNFkxQDB:L3G+
C3H10T1/2	M3G4[GZ2dmO2aX;uJIF{e2G7			NH;rZVlKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygV2hJKGGldHn2ZZRm\CCyYYToe4F6KGmwIH3veZNmKEN\|SEGwWFEwOiClZXzsd{Bie3Onc4Pl[EBieyCVQVetbY5lfWOnZDDj[YxtKGSrZn\ldoVvfGmjdHnvckBjgSCjbHvhcIlv\SCyaH;zdIhifGG\|ZTDhd5NigSxiSVO1NEA:KDBwNjFOwG0v	M4fzb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUmyO\|g5Lz5{MUW5Nlc5QDxxYU6=
C3H10T1/2	NIToXIFHfW6ldHnvckBie3OjeR?=		M4[4PVYhcHK\|	MYTJcohq[mm2aX;uJI9nKFODRz3pcoR2[2WmIHTp[oZmemWwdHnheIlwdiCxZjDtc5V{\SCvZYPlcoNpgW2jbDDwcJVzcXCxdHXueEBEO0hzMGSxM\|Ih[2WubIOgeI8h[WytYXzpcoUheGixc4DoZZRie2VicH;zbZRqfmVib3Xz[Y9jdGG\|dIOgZYZ1\XJiNjDodpMtKEmFNUCgQUAxNjZ{IN88UU4>	MkDHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{Nki1OVEoRjJ{Mk[4OVUyRC:jPh?=
ASZ001	M3T3WWZ2dmO2aX;uJIF{e2G7		NXnLZm9RPDhiaILz	M1PoNmlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIHnuJI1wfXOnIFHTXlAxOSClZXzsd{Bie3Onc4Pl[EBieyCmb4fudoVofWyjdHnvckBw\iCJbHmxJI1TVkFiZYjwdoV{e2mxbjDh[pRmeiB2ODDodpMh[nliUmStVGNTKGGwYXz5d4l{NCCLQ{WwJF0hOC54NjFOwG0v	M13SPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUCwO\|E3Lz5{NEmwNFcyPjxxYU6=
M210B4	NV;LVGxoTnWwY4Tpc44h[XO\|YYm=		MVqyOEBpenN?	NV[5O4Q1UW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegbY4hdW:3c3WgUVIyOEJ2IHPlcIx{KGG\|c3Xzd4VlKGG\|IHTve45z\We3bHH0bY9vKG:oIFfsbVEhdVKQQTDlfJBz\XO\|aX;uJIFnfGW{IEK0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXNuIFnDOVAhRSByLkig{txONg>?	MlHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3NVYoRjJ2OUCwO\|E3RC:jPh?=
medulloblastoma cells	M{PPS2FvfGmycn;sbYZmemG2aY\lJIF{e2G7			M2\iSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2VibXXkeYxtd2KuYYP0c41iKGOnbHzzJIhiemKxcnnu[{Bp\XSncn;6fYdwfXNicITjbFEh\2WwZTDifUBOXFRiYYPzZZktKEWFNUCgQUAyKM7:TT6=	NULidoR6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVc3OzFpPkG3OFE4PjNzPD;hQi=>
CHO	MoTxSpVv[3Srb36gZZN{[Xl?			M{LhWGFvfGGpb37pd5Qh[WO2aY\peJkh[XRibX;1d4UhW22xIILlZ4VxfG:{IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[nliW{PIYWhpNUGpIHLpcoRqdmdiYYPzZZktKEmFNUCgQUAyNjJizszNMi=>	NUHQ[2ZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwPVE2PTlpPkG5NFkyPTV7PD;hQi=>
Shh-Light2	M2DI[2Z2dmO2aX;uJIF{e2G7		NY\CSoFFPDhiaILz	M2HvdWlvcGmkaYTpc44hd2ZiU1jIJIlvKG2xdYPlJHNpcC2OaXfoeFIh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IFfsbVEhemWyb4L0[ZIh\2WwZTDhd5NigSCrbjDwdoV{\W6lZTDv[kBUSUduIFnDOVAhRSBzLkOxNkDPxE1w	MmnmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3NEG0PVAoRjF7NUSxOFkxRC:jPh?=
MEF	MXzGeY5kfGmxbjDhd5NigQ>?			M4S4UWlvcGmkaYTpc44hd2ZiU33vJIlvKG2xdYPlJHB1[2hvZHXmbYNq\W62IF3FSkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGPobE1qdmS3Y3XkJGdtcTFidILhcpNkemmydHnvcoFtKGGldHn2bZR6NCCLQ{WwJF0hOS53IN88UU4>	MkH6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyN{S1OFEoRjJ\|MEe0OVQyRC:jPh?=
MEF	Mo\YSpVv[3Srb36gZZN{[Xl?		NYX1SVhqOzBiaILz	NWjUS49[UW6qaXLpeIlwdiCxZjDTcY8hcW5ibX;1d4UhWHSlaD3k[YZq[2mnboSgUWVHKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gV4hpNWmwZIXj[YQhT2yrLYLld5BwdnOrdnWgZoV1[WejbHHjeI9{cWSjc3WgZYN1cX[rdImgZYZ1\XJiM{CgbJJ{KGK7IFLleIFIVG9iYYPzZZktKEmFNUCgQUAyNjlizszNMi=>	NUi2ZW5QRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO\|Q2PDFpPkKzNFc1PTRzPD;hQi=>
neural precursor cells	NF3PWFVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=			NGPC[nBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIH7leZJidCCycnXjeZJ{d3JiY3XscJMh[nliY3;sc456KG[xcn3heIlwdiCjc4PhfUwhTUN3MDC9JFE{NjR2IN88UU4>	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzN{[zNUc,OTd2MUe2N\|E9N2F-
U87	MlPhR5l1d3SxeHnjbZR6KGG\|c3H5			NY\nWZdxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTh5IHPlcIx{KGG\|c3Xzd4VlKGG\|II\pZYJqdGm2eTDpckBxemW\|ZX7j[UBw\iCkZYThJIdtfWO3cn;ubYRie2VuIFnDOVAhRSBzNT61JO69VS5?	M4PuelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUG2PVA1Lz5{MEGxOlkxPDxxYU6=
U87MG	NGHwV3pCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NFrlU3Q4OiCqcoO=	MV;BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFV6N13HJINmdGy\|IHHmeIVzKDd{IHjyd{BjgSCPVGOgZZN{[XluIFnDOVAhRSB{Mj61JO69VS5?	NEnuN\|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKyOlY2Pyd-MkKyNlY3PTd:L3G+
A549	M3;O[GFvfGmlYX7j[ZIh[XO\|YYm=			NGDl[3ZCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{BjgSCPVGOgZZN{[XluIFnDOVAhRSB2OTFOwG0v	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ{MUi3Nkc,OTh{MkG4O\|I9N2F-
DU145	NXHiOZNIT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=	MoXGOUB2VQ>?	M4rYUlk3KGi{cx?=	NEDPZYJIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBFXTF2NTDj[YxteyCjdDC1JJVOKGGodHXyJFk3KGi{cx?=	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2OUGyOUc,OTh{NEmxNlU9N2F-
DU145	NG\odHJIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	MnzoNVAhfU1?	NV\xcFR2QTZiaILz	NHnHN29Iem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBFXTF2NTDj[YxteyCjdDCxNEB2VSCjZoTldkA6PiCqcoO=	NID5Vpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0PVEzPSd-MUiyOFkyOjV:L3G+
HEK293	NHLQdJpHfW6ldHnvckBie3OjeR?=			NGLiSpRKdmirYnn0bY9vKG:oIHLleIEh\2GuYXP0c5Nq\GG\|ZTDpckBJTUt{OUOgZ4VtdHN?	M4i0O\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEm0O\|Y3Lz5zN{S5OFc3PjxxYU6=
22Rv	NIrsV5JHfW6ldHnvckBie3OjeR?=			MXPS[YR2[3Srb36gc4Yh\XiycnXzd4lwdiCxZjDQWGNJKG2UTlGgbY4hcHWvYX6gNlJTfiClZXzsdy=>	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR7NEe2Okc,OTd2OUS3OlY9N2F-
PANC1	NGrHXo5HfW6ldHnvckBie3OjeR?=	M3rySlAvOiC3TR?=	NEjKV44zPCCqcoO=	Ml;JTY5pcWKrdHnvckBw\iCKaD;HUGkyNW2nZHnheIVlKFCWQ1igcXJPSSCneIDy[ZN{cW:wIHnuJIh2dWGwIGDBUmMyKGOnbHzzJIF1KDBwMjD1UUBi\nSncjCyOEBpenNiYomgVnQuWEOU	Mmj6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUCxO\|AoRjJyNEWwNVcxRC:jPh?=
PANC1	NF7tWY9HfW6ldHnvckBie3OjeR?=	NEfaWFExNjRidV2=	M4H4cVI1KGi{cx?=	MoPRTY5pcWKrdHnvckBw\iCKaD;HUGkyNW2nZHnheIVlKFCWQ1igcXJPSSCneIDy[ZN{cW:wIHnuJIh2dWGwIGDBUmMyKGOnbHzzJIF1KDBwNDD1UUBi\nSncjCyOEBpenNiYomgVnQuWEOU	MorzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUCxO\|AoRjJyNEWwNVcxRC:jPh?=
Shh Light2	MUXGeY5kfGmxbjDhd5NigQ>?	M1W1UVYvOjVidV2=	Ml7SN\|AhcHK\|	M{LQZ2lvcGmkaYTpc44hd2ZiTj3wZYxucXSxeXzheIVlKFOqaDDpckBud3W\|ZTDTbIghVGmpaISyJINmdGy\|IHH0JFYvOjVidV2gZYZ1\XJiM{CgbJJ{KGK7IH\pdoVndHlibIXjbYZmemG\|ZTDy[ZBwenSncjDn[Y5mKGG\|c3H5	NFzWb2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUG1NVc{OSd-MUmxOVE4OzF:L3G+
U87MG	NHTqPHVHfW6ldHnvckBie3OjeR?=	M1;ZTlExKHWP	MXy0JIhzew>?	NXiyemxOUW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBpfW2jbjDVPFdOTyClZXzsd{Bie3Onc4Pl[EBieyCmb4fuJJJm\3WuYYTpc44hd2ZiR3zpNUBifCBzMDD1UUBi\nSncjC0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN?	M1\RUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkK2OlU4Lz5{MkKyOlY2PzxxYU6=
HEK293	NEPGWIFHfW6ldHnvckBie3OjeR?=	NXPvfXZkPSC3TR?=	NUXOUXVOOTBiaILz	NXThems4TGm\|cHzhZ4Vu\W62IH;mJGJQTEmSWT3jfYNtd3CjbXnu[UBnem:vIHj1cYFvKFOvbzDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHH0JFUhfU1ibXXhd5Vz\WRiYX\0[ZIhOTBiaILzJIJ6KESDUFmgd5RicW6rbnegZoF{\WRiZnz1c5Jme2OnbnPlJI1q[3Kxc3PvdIlkKGG\|c3H5	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd\|NkC2N{c,Ojd5M{[wOlM9N2F-
HEK293	M2LBc2Z2dmO2aX;uJIF{e2G7	M4GyflUhfU1?	NHr3cW0yOCCqcoO=	NWTtOGwyTGm\|cHzhZ4Vu\W62IH;mJGJQTEmSWT3jfYNtd3CjbXnu[UBnem:vIHj1cYFvKFOvbzDlfJBz\XO\|ZXSgbY4hUEWNMkmzJINmdGy\|IHH0JFUhfU1ibXXhd5Vz\WRiYX\0[ZIhOTBiaILzJIJ6KE[DQ2OgZY5idHm\|aYO=	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd\|NkC2N{c,Ojd5M{[wOlM9N2F-
C3H10T1/2	NFXVPFNHfW6ldHnvckBie3OjeR?=	MnvxOVUvPSC2bzC0OVAxKG6P		MmS1R49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDTcY8hcW5ibX;1d4UhSzOKMUDUNU8zKGOnbHzzJIF{e2W\|c3XkJIF{KGmwaHnibZRqd25ib3[gV2FINWmwZIXj[YQhT2yrMTD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYYSgOVUvPSC2bzC0OVAxKG6PIHL5JJFRS1JiYX7hcJl{cXN?	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB5NEW0NUc,OjNyN{S1OFE9N2F-
U2OS	MVPGeY5kfGmxbjDhd5NigQ>?	NIH0dFg2KHWP	NIPEO2I3KGi{cx?=	MXzBcpRi\2:waYP0JIFkfGm4aYT5JIF1KGOqaX3ldolkKFOvbzC2N\|MhdXW2YX70JIV5eHKnc4Pl[EBqdiCXMl;TJINmdGy\|IHPv[ZhxemW\|c3nu[{Bj\XSjIHHydoV{fGmwMj3HSnAh[XO\|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBqdnS{YXPlcIx2dGG{IHLleIEh[XK{ZYP0bY4zNUeIUDDh[4dz\WejdHWg[o9zdWG2aX;uJIF1KDVidV2gZYZ1\XJiNjDodpMh[nliY3;u[o9k[WxibXnjdo9{[2:yeR?=	M1rPd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|ME[zOVIzLz5{M{C2N\|UzOjxxYU6=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	NF-κB / Cyclin D1 / MMP2 / MMP9; PubMed: 23599805 Oncol Lett Whole cell lysates were prepared, and 50 μg proteins were resolved using SDS-PAGE, followed by immunoblotting with the indicated specific antibodies against NF-κB, cyclin D1, MMP2 and MMP9. Gli1 / TGF-β1 / CXCR4; PubMed: 26137001 Oncol Lett Western blotting demonstrated that Gli1, TGF-β1 and CXCR4 protein expression levels were downregulated in the AGS cells treated with cyclopamine for 24 h. Snail / E-cadherin / Slug / Vimentin ; PubMed: 26859575 Oncotarget SW1736 and WRO82 cells were incubated in the presence of vehicle (0.5% DMSO) or the indicated concentrations of cyclopamine or GANT61 for 72 hr. The cells were harvested and analyzed for the expression of several genes involved in EMT.	23599805 26137001 26859575
Immunofluorescence	Vimentin; PubMed: 24553082 Cancer Biol Ther Vimentin protein expression was reduced following treatment with cyclopamine (20 μmol/L; 72 h), detected by immunocytochemistry. PCNA / β-catenin; PubMed: 28747625 Sci Rep Immunofluorescence images demonstrating the distribution of PCNA or β-catenin in EH cells pre-treated with cyclopamine in presence and absence of estrogen for 24 h. Experiments were repeated at least three times.	24553082 28747625

体内研究

Cyclopamine 每天按50 mg/kg剂量处理小鼠，持续22天，消除HUCCT1移植瘤，且没有明显副作用。^[2] Cyclopamine 按1.2 mg剂量处理Panc 05.04和 L3.6sl驱动的肿瘤7天，前者诱导明显的肿瘤细胞凋亡，后者降低肿瘤质量，降低达50-60%，但是作用于BxPC3-SMOlow肿瘤没有效果。^[3] Cyclopamine单独处理E3LZ10.7 和L3.6pl移植瘤，显著抑制肿瘤转移，且和 Gemcitabine联用处理，完全废除转移。^[4]

激酶实验：^[1]	- 合并激酶实验: Gli-Luc实验测定Hh 信号通路末期, 即使用荧光素酶进行读数，测定Gli的转录调控。Cyclopamine 在DMSO中连续稀释用于实验，然后加到空的实验板上。TM3Hh12 细胞(TM3 细胞含Hh反应受体基因结构 pTA-8xGli-Luc) 悬浮在含5% FBS 和15 mM Hepes pH 7.3的F12 Ham’s/DMEM (1:1)培养基上, 加到实验板上，与Cyclopamine在37^oC下温育30分钟左右。1 nM Hh-Ag 1.5 加到实验板上，然后在37^oC下温育。48小时后，在实验板中加入Bright-Glo或MTS，然后在492 nm处测定发光度或吸光度。通过分析反应曲线，使用R统计软件包测定IC50值。
细胞实验：^[2]	- 合并 Cell lines: SEG1, OE33, KYAE, KYSE180, SNU1, AGS, SNU16, NCI-N-87, HUCCT1, PANC1, PL5, PL6, BXPC3, HS766T, KYSE150, GBD1, DLD1, 和HCT116 Concentrations: 溶于DMSO, 终浓度为3 μM Incubation Time: 4天 Method: 在96孔板上，用Cyclopamine 处理细胞。通过MTS实验测定细胞活力。进行CellTiter96比色测定，进行2和4天，然后在490 nm处通过测定光密度而测定存活细胞。 (Only for Reference)
动物实验：^[2]	- 合并 Animal Models: 皮下注射HUCCT1细胞的无胸腺裸鼠 Dosages: 50 mg/kg/day Administration: 皮下注射处理 (Only for Reference)

参考文献

[4] Feldmann G, et al. Cancer Res, 2007, 67(5), 2187-2196.

- 合并

溶解度 (25°C)

体外	DMF	10 mg/mL warmed (24.29 mM)
	Ethanol	2 mg/mL warmed (4.85 mM)
	DMSO	Insoluble

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Cyclopamine SDF

分子量	411.62
化学式	C₂₇H₄₁NO₂
CAS号	4449-51-8
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	11-deoxojervine

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
How to reconstitute the compound for in vivo use in mice?
回答：
One paper dissolved this drug in DMSO, and diluted in saline: Berman DM, et al. Nature, 2003, 425(6960), 846-851. Alternatively, you can try this vehicle: 10% DMSO+30% PEG 300+5% Tween 80+ddH2O for P.O. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG300 and Tween, after they mixed well, dilute with water.

Hedgehog/Smoothened Signaling Pathway Map

Hedgehog/Smoothened Inhibitors with Unique Features

选择性强的Smoothened抑制剂

PF-5274857 : Smoothened-selective, IC50=5.8 nM.
用于临床的Smoothened抑制剂

LDE225 (NVP-LDE225,Erismodegib) : Phase III for Hh-pathway activated relapsed Medulloblastoma (MB).
最新的Smoothened抑制剂

GANT61 : Inhibitor for GLI1 as well as GLI2-induced transcription, inhibits hedgehog with IC50 of 5 μM, displays selectivity over other pathways, such as TNF and glucocorticoid receptor gene transactivation.
Smoothened激活剂

Purmorphamine : Directly binds and activates Smoothened, and blocks BODIPY-cyclopamine binding to Smo with IC50 of ~ 1.5 μM.

研究领域

产品类型

定制您的化合物库

Cyclopamine

客户使用Selleck生产的Cyclopamine发表文献85篇：

产品安全说明书

Hedgehog/Smoothened抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Cyclopamine SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

技术支持

常见问题及建议解决方法

Hedgehog/Smoothened Signaling Pathway Map

Hedgehog/Smoothened Inhibitors with Unique Features

相关Hedgehog/Smoothened产品