Cyclopamine

For research use only. Not for use in humans.

目录号:S1146 别名: 11-deoxojervine 中文名称:环巴胺

Cyclopamine Chemical Structure

CAS No. 4449-51-8

Cyclopamine (11-deoxojervine)是一种特异性Hedgehog (Hh)信号通路拮抗剂,作用于Smoothened (Smo),在TM3Hh12细胞中IC50为46 nM。

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客户使用Selleck生产的Cyclopamine发表文献74篇:

产品安全说明书

Hedgehog/Smoothened抑制剂选择性比较

生物活性

产品描述 Cyclopamine (11-deoxojervine)是一种特异性Hedgehog (Hh)信号通路拮抗剂,作用于Smoothened (Smo),在TM3Hh12细胞中IC50为46 nM。
靶点
Smoothened [1]
(TM3Hh12 cells)
46 nM
体外研究

Cyclopamine 抑制Hedgehog信号通路, IC50 为46 nM, 且在[3H]Hh-Ag结合实验中,作用于CHO-K1细胞,抑制人类Smo受体活性,IC50为 280 nM。[1] Cyclopamine作用于表达Patched (PTCH) mRNA的肠源性肿瘤细胞,显著抑制 Hedgehog通路活性,这种作用存在剂量依赖性,且浓度为3 μM时,抑制肿瘤细胞生长,抑制达75-95%, 但是作用于不表达PTCH mRNA的结肠癌细胞没有效果,说明Cyclopamine 特定作用于Hedgehog 通路,且没有毒性。[2]通过与Smo直接作用而抑制Hedgehog信号,Cyclopamine (10 μM) 抑制SMOhigh Cyclopamine-反应细胞系L3.6sl和 Panc 05.04增殖,抑制达 75-80%,且使凋亡率提高2.5到 3.5倍,但是不影响BxPC3-SMOlow 细胞系。[3] Cyclopamine 处理E3LZ10.7细胞系,显著降低Snail mRNA,且提供E-钙粘着蛋白转录。Cyclopamine处理Hedgehog依赖型 L3.6pl 细胞,显著抑制浸润型细胞, 使转移细胞降低500多倍,但是对Hedgehog非依赖性细胞系Panc-1没有影响。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
OS-RC-2 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M36wcWlEPTB;NT64OlY3KM7:TR?= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
DOHH-2 NVrE[nlbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLwb4pLUUN3ME25MlM2Pjh7IN88US=> M33VelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
no-10 NHfrUJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTlwOUCzPUDPxE1? NHWxe3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
LS-513 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLiN2VKSzVyPUGxMlM2PDdizszN MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ALL-PO M1fGbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTmR|V1UUN3ME2xNU44PzN2IN88US=> M2eySFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
8-MG-BA NYnTZolTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjYeIlKSzVyPUGzMlEyOjNizszN NYWyXIlMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
RPMI-8402 MoO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoTzTWM2OD1zNT64OVM4KM7:TR?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
EoL-1-cell NGTWTmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTF6LkW5OFgh|ryP NHy0W2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NALM-6 NUDOVWVXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonaTWM2OD1zOT6wNVY4KM7:TR?= M4r0blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
DEL MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1ewdWlEPTB;MkCuNVQ4OSEQvF2= Mnr3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SR Mkm0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTJ|Lk[3NVUh|ryP NW\ES|Y2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
697 M2TUOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\Qc5VKSzVyPUK2MlYyPTVizszN MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
COLO-829 NHGxVGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXNTWM2OD1{Nj64OFg{KM7:TR?= MmrLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
EVSA-T MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfFU2JKSzVyPUK3MlU2PjFizszN M{[zN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ATN-1 NX73flhVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVTJR|UxRTNzLkKzNlkh|ryP NF\VNmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
L-363 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYq5NHRqUUN3ME2zNU44PDZzIN88US=> MlyzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LAMA-84 NXH2T3oyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmCyTWM2OD1|Mj61NlEyKM7:TR?= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NOS-1 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjNWIliUUN3ME2zOE4zQTV4IN88US=> MmHEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
BB30-HNC M37JT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{WyNWlEPTB;M{SuN|MxPiEQvF2= NHvnR3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
BC-1 NVfUZXJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;GfYpOUUN3ME2zO{46PzR4IN88US=> NH\FPWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
IST-SL2 M3TOZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TrbWlEPTB;M{iuNlI1KM7:TR?= M4PLXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
D-392MG MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnCUZpKUUN3ME20NE4zOjF3IN88US=> MlnsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
no-11 NVfpSoc1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4flNWlEPTB;NECuOVUzOSEQvF2= NUG4XY1yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LC4-1 NXzJWI1KT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTRyLki3NVYh|ryP NV:1OVUzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
A388 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vzcGlEPTB;NEKuOVg1QCEQvF2= M33BbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NTERA-S-cl-D1 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\4XJBuUUN3ME20Nk44ODd2IN88US=> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
CESS MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LNcWlEPTB;NESuNlI{OiEQvF2= NVjxWJNqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
RS4-11 NYjGWZNNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTR7LkC5N|gh|ryP M{K2XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MS-1 NHjvcW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTVyLkmzOVEh|ryP NHPiW4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
CTV-1 NULDbnk6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTVzLkC3OEDPxE1? NIjZW2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
D-502MG NX\Ke5FoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUWxWJZnUUN3ME21NU43OjdzIN88US=> NG\FTIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ML-2 NIPlemJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXrRTXp7UUN3ME21Nk46OTl3IN88US=> M3rGblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SK-NEP-1 NYrUN3UxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjyNW92UUN3ME21N{4{QTJ|IN88US=> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LOXIMVI MlrPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DweWlEPTB;NUOuOVg5PCEQvF2= MmjaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
DJM-1 MkLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrHSnBKSzVyPUW2MlM{QTFizszN NV62e2ZQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
GI-1 NUDC[4FnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLnTWM2OD13Nj62NVQ6KM7:TR?= Ml\1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
IST-MES1 NWKweFc4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYTk[5o4UUN3ME22NE42PDl|IN88US=> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MV-4-11 NGqwfplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXieXBKSzVyPU[wMlY2OzhizszN M2TDXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
OVCAR-4 NXWzdHBnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{L4XWlEPTB;NkOuOVY2PyEQvF2= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KE-37 NIL1TIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGj3XndKSzVyPU[2MlI3PjhizszN NV3XTnlrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
D-542MG MnfuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHod3RKSzVyPU[4MlQyOzVizszN M2W3[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MHH-PREB-1 NEPIRVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTd{Lki0OFEh|ryP MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
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MC-CAR MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M16xWmlEPTB;MkCyMlI2OyEQvF2= NXe1SGdSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SNB75 M1PiZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzUTWM2OD1{MkGuPVQh|ryP MmX4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ES4 NGXMV3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MonqTWM2OD1{MkOuO|g{KM7:TR?= NXLVXllKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KARPAS-422 MoCxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MY\JR|UxRTJ{OD6zOVIh|ryP MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-H1648 Ml;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTJb5BKSzVyPUKyPU41QDlizszN NFv5RYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ES6 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTJ|OT60N{DPxE1? MmfNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KNS-81-FD NH;xflZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfFTWM2OD1{NEGuNVk4KM7:TR?= NYLmRmdYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
JAR MmO3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYTEVJVRUUN3ME2yOVYvOjJ3IN88US=> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NB1 NGTCeWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTJ4MD61NVYh|ryP M{L4O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
D-336MG NELSSYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTGd5VQUUN3ME2yOlAvPjl6IN88US=> NGL6OlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
BC-3 M2rtR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkS2TWM2OD1{NkWuNVc5KM7:TR?= MmrYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HCC2218 NW\T[IZ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUXKWFJqUUN3ME2yOlYvPDF3IN88US=> M3vae|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
TE-9 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\vTWM2OD1{Nk[uOlI4KM7:TR?= NYHqcWJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LB1047-RCC MlTxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLhTWM2OD1{Nk[uO|U{KM7:TR?= MoTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
CTB-1 M4D0T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jsWmlEPTB;Mk[5Mlk4OyEQvF2= M1X3clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
NB7 M{j4S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\lTWM2OD1{N{Gg{txO NWnSRpV[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ST486 NYf3VItwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFHXbnJKSzVyPUK3O{41OTJizszN MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HCC1187 NIjUblBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;0d2lEPTB;MkiyMlgyOSEQvF2= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-SNU-16 M2jJSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFywc5FKSzVyPUK4OE4zPDhizszN MmS4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
COR-L279 MlPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTiVWdpUUN3ME2yPVEvPTh2IN88US=> MmW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ES8 MkD3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\HTlZKSzVyPUK5OE4yQDJizszN MkK4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
U-698-M NUXrXZZGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnLtTWM2OD1{OUiuNlQ{KM7:TR?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HEL NFmyN5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTNyOT6xOFkh|ryP MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
KINGS-1 M3;RTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlTNTWM2OD1|MUCuOlc1KM7:TR?= MoTDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KY821 M4TtUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DybWlEPTB;M{O2MlU6PSEQvF2= MnXHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MZ1-PC NEjj[45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjER4dKSzVyPUO0OU43OThizszN MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LS-411N MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLhNZZKSzVyPUO1OE43PiEQvF2= M4P0NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SIG-M5 NVLYTphFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHHhWZpKSzVyPUO1PU44QDJizszN M1rC[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HT NIPJT2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGT2RplKSzVyPUO2O{44OTFizszN MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HC-1 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2m3emlEPTB;M{[3Mlc5PyEQvF2= NXS4TllkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H1694 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxRTN5Mj65N|Qh|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BB65-RCC NHHyWnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTN5Nj6yOFUh|ryP NGj4boM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
HAL-01 NX7GXWdFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXZTWM2OD1|N{muPFM5KM7:TR?= NVnDO3V5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ARH-77 M2\keWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXLSZZxUUN3ME2zPVQvODB6IN88US=> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MZ7-mel NV\NRZcxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3XTWM2OD1|OUeuNlM{KM7:TR?= MmnvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SIMA NUDyfmVKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHyTWM2OD12MEOuPVM{KM7:TR?= NGi4fpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
DG-75 M4fMNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfOSmpKSzVyPUSxOU43QThizszN NUS4U3ZpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HUTU-80 M{fZVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4\6T2lEPTB;NEG5MlE5PSEQvF2= NUjVTVVlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KNS-42 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4Pq[2lEPTB;NEK1MlgyPSEQvF2= NYGxWXU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SH-4 NIH0S3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlyyTWM2OD12MkeuOVY2KM7:TR?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
L-540 NYTKUHFwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfUUHg2UUN3ME20N|EvODNzIN88US=> NFy4cXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NB10 NFzRWGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTsTWM2OD12NEGuNlM1KM7:TR?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ES1 NYrvXJh4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHJTWM2OD12NUKuO|U{KM7:TR?= NWnrZ|JJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KMOE-2 NVf4PZlJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVHJR|UxRTR3Nj63NVEh|ryP M3r0SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MC116 NYWzfYxuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETEbmpKSzVyPUS1PE4yOTZizszN NUTPXZFKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
RCC10RGB MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4j1SWlEPTB;NE[wMlAxPSEQvF2= NETjcow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
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Raji NGPoW5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVLJR|UxRTR4OD6xOFMh|ryP NEnKb4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
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Shh-light2 NGXNOJVHfW6ldHnvckBie3OjeR?= MoTPTY5pcWKrdHnvckBw\iCVbX:tcYVlcWG2ZXSgTIghe2mpbnHsbY5oKGmwIHj1cYFvKFOqaD3sbYdpfDJiY3XscJMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyIE2gNE4{KM7:TT6= M17PdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[4OVUyLz5{MkK2PFU2OTxxYU6=
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C3H10T1/2 NYHQVGJSTnWwY4Tpc44h[XO|YYm= NFvYOnVKdmirYnn0bY9vKG:oIF6teIVzdWmwYXygV2hJKGGldHn2ZZRm\CCyYYToe4F6KGmwIH3veZNmKEN|SEGwWFEwOiClZXzsd{Bie3Onc4Pl[EBieyCVQVetbY5lfWOnZDDj[YxtKGSrZn\ldoVvfGmjdHnvckBjgSCjbHvhcIlv\SCyaH;zdIhifGG|ZTDhd5NigSxiSVO1NEA:KDBwNjFOwG0v NUXK[41VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1PVI4QDhpPkKxOVkzPzh6PD;hQi=>
C3H10T1/2 NEHvfYdHfW6ldHnvckBie3OjeR?= NIL6R5Y3KGi{cx?= MV7Jcohq[mm2aX;uJI9nKFODRz3pcoR2[2WmIHTp[oZmemWwdHnheIlwdiCxZjDtc5V{\SCvZYPlcoNpgW2jbDDwcJVzcXCxdHXueEBEO0hzMGSxM|Ih[2WubIOgeI8h[WytYXzpcoUheGixc4DoZZRie2VicH;zbZRqfmVib3Xz[Y9jdGG|dIOgZYZ1\XJiNjDodpMtKEmFNUCgQUAxNjZ{IN88UU4> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4OEW1NUc,OjJ{Nki1OVE9N2F-
ASZ001 NX\EWWdETnWwY4Tpc44h[XO|YYm= NHz4R5c1QCCqcoO= MlvFTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC|aXfuZYxqdmdiaX6gcY92e2ViQWPaNFAyKGOnbHzzJIF{e2W|c3XkJIF{KGSxd37y[Yd2dGG2aX;uJI9nKEeuaUGgcXJPSSCneIDy[ZN{cW:wIHHmeIVzKDR6IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZMtKEmFNUCgQUAxNjZ4IN88UU4> NEjNVYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFcyPid-MkS5NFA4OTZ:L3G+
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medulloblastoma cells NEHXT|JCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MkXvRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCvZXT1cIxw[myjc4TvcYEh[2WubIOgbIFz[m:{aX7nJIhmfGW{b4r5[492eyCydHPoNUBo\W6nIHL5JG1VXCCjc4PhfUwhTUN3MDC9JFEh|ryPLh?= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzRzN{[zNUc,OTd2MUe2N|E9N2F-
CHO M331NWZ2dmO2aX;uJIF{e2G7 MknZRY51[WexbnnzeEBi[3Srdnn0fUBifCCvb4Xz[UBUdW9icnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{BjgSCdM1jdTIguSWdiYnnu[Ilv\yCjc4PhfUwhUUN3MDC9JFEvOiEQvF2u M4HBVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEmxOVU6Lz5zOUC5NVU2QTxxYU6=
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neural precursor cells M1vB[mFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NUfveWI{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDu[ZVz[WxicILlZ5Vze2:{IHPlcIx{KGK7IHPvcI9vgSCob4LtZZRqd25iYYPzZZktKEWFNUCgQUAyOy52NDFOwG0v NV\1eWZzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVc3OzFpPkG3OFE4PjNzPD;hQi=>
U87 NUTsfWMyS3m2b4TvfIlkcXS7IHHzd4F6 NWSyXZNyS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTh5IHPlcIx{KGG|c3Xzd4VlKGG|II\pZYJqdGm2eTDpckBxemW|ZX7j[UBw\iCkZYThJIdtfWO3cn;ubYRie2VuIFnDOVAhRSBzNT61JO69VS5? M3H4flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUG2PVA1Lz5{MEGxOlkxPDxxYU6=
U87MG MV7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M{O2NVczKGi{cx?= NEH2NodCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGW4O21IKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHMh[XO|YYmsJGlEPTBiPTCyNk42KM7:TT6= Mki3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{Mk[2OVcoRjJ{MkK2OlU4RC:jPh?=
A549 NYPyR3p5SW62aXPhcoNmeiCjc4PhfS=> NYK3c|VHSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[nliTWTTJIF{e2G7LDDJR|UxKD1iNEmg{txONg>? M17zeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkKxPFczLz5zOEKyNVg4OjxxYU6=
DU145 MoH0S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NW\VXVQ4PSC3TR?= NGjrOII6PiCqcoO= MWTHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDEWVE1PSClZXzsd{BifCB3IIXNJIFnfGW{IEm2JIhzew>? NEi4bmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0PVEzPSd-MUiyOFkyOjV:L3G+
DU145 M1P0dWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NF3YNnAyOCC3TR?= MUW5OkBpenN? NEfrWpNIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBFXTF2NTDj[YxteyCjdDCxNEB2VSCjZoTldkA6PiCqcoO= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2OUGyOUc,OTh{NEmxNlU9N2F-
HEK293 NW\US4d4TnWwY4Tpc44h[XO|YYm= NGLsdFlKdmirYnn0bY9vKG:oIHLleIEh\2GuYXP0c5Nq\GG|ZTDpckBJTUt{OUOgZ4VtdHN? M{DqUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEm0O|Y3Lz5zN{S5OFc3PjxxYU6=
22Rv MV;GeY5kfGmxbjDhd5NigQ>? NXPVUm1RWmWmdXP0bY9vKG:oIHX4dJJme3Orb36gc4YhWFSFSDDtVm5CKGmwIHj1cYFvKDJ{Uo[gZ4VtdHN? NGTnfpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S5OFc3Pid-MUe0PVQ4PjZ:L3G+
PANC1 M{DxfmZ2dmO2aX;uJIF{e2G7 NXHLV|AxOC5{IIXN NXv2V4VMOjRiaILz NWf4RWpRUW6qaXLpeIlwdiCxZjDIbE9IVElzLX3l[IlifGWmIGDUR2ghdVKQQTDlfJBz\XO|aX;uJIlvKGi3bXHuJHBCVkNzIHPlcIx{KGG2IECuNkB2VSCjZoTldkAzPCCqcoOgZpkhWlRvUFPS NIfjWI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NFE4OCd-MkC0OVAyPzB:L3G+
PANC1 M2fnNWZ2dmO2aX;uJIF{e2G7 NED0WJcxNjRidV2= MUiyOEBpenN? NETUd2JKdmirYnn0bY9vKG:oIFjoM2dNUTFvbXXkbYF1\WRiUGTDTEBuWk6DIHX4dJJme3Orb36gbY4hcHWvYX6gVGFPSzFiY3XscJMh[XRiMD60JJVOKGGodHXyJFI1KGi{czDifUBTXC2SQ2K= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR3MEG3NEc,OjB2NUCxO|A9N2F-
Shh Light2 M4LGe2Z2dmO2aX;uJIF{e2G7 MoDCOk4zPSC3TR?= NG\EUGw{OCCqcoO= NX7MSmlzUW6qaXLpeIlwdiCxZjDOMZBidG2rdH;5cIF1\WRiU3joJIlvKG2xdYPlJHNpcCCOaXfoeFIh[2WubIOgZZQhPi5{NTD1UUBi\nSncjCzNEBpenNiYomg[olz\W[ueTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> Mnz0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzNUG3N|EoRjF7MUWxO|MyRC:jPh?=
U87MG NVrEd4ZHTnWwY4Tpc44h[XO|YYm= NGPyb2syOCC3TR?= NUDD[GZzPCCqcoO= NV3Wc3NvUW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBpfW2jbjDVPFdOTyClZXzsd{Bie3Onc4Pl[EBieyCmb4fuJJJm\3WuYYTpc44hd2ZiR3zpNUBifCBzMDD1UUBi\nSncjC0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN? NHW5PZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkKyOlY2Pyd-MkKyNlY3PTd:L3G+
HEK293 NHXD[mpHfW6ldHnvckBie3OjeR?= MmTCOUB2VQ>? MW[xNEBpenN? MYHEbZNxdGGlZX3lcpQhd2ZiQl;ETXB[NWO7Y3zvdIFucW6nIH\yc40hcHWvYX6gV41wKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XRiNTD1UUBu\WG|dYLl[EBi\nSncjCxNEBpenNiYomgSGFRUSC|dHHpcolv\yCkYYPl[EBndHWxcnXzZ4Vv[2VibXnjdo9{[2:yaXOgZZN{[Xl? Ml74QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5M{[wOlMoRjJ5N{O2NFY{RC:jPh?=
HEK293 Mnj6SpVv[3Srb36gZZN{[Xl? NGr6V2w2KHWP NV\XZZN7OTBiaILz NFXvVJJFcXOybHHj[Y1mdnRib3[gRm9FUVC\LXP5Z4xweGGvaX7lJIZzd21iaIXtZY4hW22xIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYSgOUB2VSCvZXHzeZJm\CCjZoTldkAyOCCqcoOgZpkhTkGFUzDhcoFtgXOrcx?= NIjkW4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{ezOlA3Oyd-Mke3N|YxPjN:L3G+
C3H10T1/2 M4D2bWZ2dmO2aX;uJIF{e2G7 MlzLOVUvPSC2bzC0OVAxKG6P NWLWR5hLS2:vcHX0bZRqfmViaX7obYJqfGmxbjDv[kBUdW9iaX6gcY92e2ViQ{PINVBVOS9{IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhW0GJLXnu[JVk\WRiR3zpNUB1emGwc3PybZB1cW:wYXygZYN1cX[rdImgZZQhPTVwNTD0c{A1PTByIH7NJIJ6KHGSQ2KgZY5idHm|aYO= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB5NEW0NUc,OjNyN{S1OFE9N2F-
U2OS NVTBNJNHTnWwY4Tpc44h[XO|YYm= NXSzTWpkPSC3TR?= M4XETlYhcHK| NGrXRWhCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IHPobY1memmlIGPtc{A3OzNibYX0ZY51KGW6cILld5Nm\CCrbjDVNm9UKGOnbHzzJINw\XiycnXzd4lv\yCkZYThJIFzemW|dHnuNk1ITlBiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCrboTyZYNmdGy3bHHyJIJmfGFiYYLy[ZN1cW5{LVfGVEBi\2e{ZXfheIUh\m:{bXH0bY9vKGG2IEWgeW0h[W[2ZYKgOkBpenNiYomgZ49v\m:lYXygcYlkem:|Y3;wfS=> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4M{WyNkc,OjNyNkO1NlI9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
NF-κB / Cyclin D1 / MMP2 / MMP9; 

PubMed: 23599805     


Whole cell lysates were prepared, and 50 μg proteins were resolved using SDS-PAGE, followed by immunoblotting with the indicated specific antibodies against NF-κB, cyclin D1, MMP2 and MMP9. 

Gli1 / TGF-β1 / CXCR4; 

PubMed: 26137001     


Western blotting demonstrated that Gli1, TGF-β1 and CXCR4 protein expression levels were downregulated in the AGS cells treated with cyclopamine for 24 h.

Snail / E-cadherin / Slug / Vimentin ; 

PubMed: 26859575     


SW1736 and WRO82 cells were incubated in the presence of vehicle (0.5% DMSO) or the indicated concentrations of cyclopamine or GANT61 for 72 hr. The cells were harvested and analyzed for the expression of several genes involved in EMT.

23599805 26137001 26859575
Immunofluorescence
Vimentin; 

PubMed: 24553082     


Vimentin protein expression was reduced following treatment with cyclopamine (20 μmol/L; 72 h), detected by immunocytochemistry.

PCNA / β-catenin; 

PubMed: 28747625     


Immunofluorescence images demonstrating the distribution of PCNA or β-catenin in EH cells pre-treated with cyclopamine in presence and absence of estrogen for 24 h. Experiments were repeated at least three times.

24553082 28747625
体内研究 Cyclopamine 每天按50 mg/kg剂量处理小鼠,持续22天,消除HUCCT1移植瘤,且没有明显副作用。[2] Cyclopamine 按1.2 mg剂量处理Panc 05.04和 L3.6sl驱动的肿瘤7天,前者诱导明显的肿瘤细胞凋亡,后者降低肿瘤质量,降低达50-60%,但是作用于BxPC3-SMOlow肿瘤没有效果。[3] Cyclopamine单独处理E3LZ10.7 和L3.6pl移植瘤,显著抑制肿瘤转移,且和 Gemcitabine联用处理,完全废除转移。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

激酶实验:

Gli-Luc实验测定Hh 信号通路末期, 即使用荧光素酶进行读数,测定Gli的转录调控。Cyclopamine 在DMSO中连续稀释用于实验,然后加到空的实验板上。TM3Hh12 细胞(TM3 细胞含Hh反应受体基因结构 pTA-8xGli-Luc) 悬浮在含5% FBS 和15 mM Hepes pH 7.3的F12 Ham’s/DMEM (1:1)培养基上, 加到实验板上,与Cyclopamine在37oC下温育30分钟左右。1 nM Hh-Ag 1.5 加到实验板上,然后在37oC下温育。48小时后,在实验板中加入Bright-Glo或MTS,然后在492 nm处测定发光度或吸光度。通过分析反应曲线,使用R统计软件包测定IC50值。
细胞实验:[2]
- 合并
  • Cell lines: SEG1, OE33, KYAE, KYSE180, SNU1, AGS, SNU16, NCI-N-87, HUCCT1, PANC1, PL5, PL6, BXPC3, HS766T, KYSE150, GBD1, DLD1, 和HCT116
  • Concentrations: 溶于DMSO, 终浓度为3 μM
  • Incubation Time: 4天
  • Method: 在96孔板上,用Cyclopamine 处理细胞。通过MTS实验测定细胞活力。进行CellTiter96比色测定,进行2和4天,然后在490 nm处通过测定光密度而测定存活细胞。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: 皮下注射HUCCT1细胞的无胸腺裸鼠
  • Dosages: 50 mg/kg/day
  • Administration: 皮下注射处理
    (Only for Reference)

溶解度 (25°C)

体外 DMF 10 mg/mL warmed (24.29 mM)
Ethanol 2 mg/mL warmed (4.85 mM)
DMSO Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 411.62
化学式

C27H41NO2

CAS号 4449-51-8
储存条件 粉状
溶于溶剂
别名 11-deoxojervine

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How to reconstitute the compound for in vivo use in mice?

  • 回答:

    One paper dissolved this drug in DMSO, and diluted in saline: Berman DM, et al. Nature, 2003, 425(6960), 846-851. Alternatively, you can try this vehicle: 10% DMSO+30% PEG 300+5% Tween 80+ddH2O for P.O. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG300 and Tween, after they mixed well, dilute with water.

Hedgehog/Smoothened Signaling Pathway Map

Hedgehog/Smoothened Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID