Cyclopamine

For research use only. Not for use in humans.

目录号:S1146 别名: 11-deoxojervine 中文名称:环巴胺

Cyclopamine Chemical Structure

CAS No. 4449-51-8

Cyclopamine (11-deoxojervine)是一种特异性Hedgehog (Hh)信号通路拮抗剂,作用于Smoothened (Smo),在TM3Hh12细胞中IC50为46 nM。

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客户使用Selleck生产的Cyclopamine发表文献102篇:

产品安全说明书

Hedgehog/Smoothened抑制剂选择性比较

生物活性

产品描述 Cyclopamine (11-deoxojervine)是一种特异性Hedgehog (Hh)信号通路拮抗剂,作用于Smoothened (Smo),在TM3Hh12细胞中IC50为46 nM。
靶点
Smoothened [1]
(TM3Hh12 cells)
46 nM
体外研究

Cyclopamine 抑制Hedgehog信号通路, IC50 为46 nM, 且在[3H]Hh-Ag结合实验中,作用于CHO-K1细胞,抑制人类Smo受体活性,IC50为 280 nM。[1] Cyclopamine作用于表达Patched (PTCH) mRNA的肠源性肿瘤细胞,显著抑制 Hedgehog通路活性,这种作用存在剂量依赖性,且浓度为3 μM时,抑制肿瘤细胞生长,抑制达75-95%, 但是作用于不表达PTCH mRNA的结肠癌细胞没有效果,说明Cyclopamine 特定作用于Hedgehog 通路,且没有毒性。[2]通过与Smo直接作用而抑制Hedgehog信号,Cyclopamine (10 μM) 抑制SMOhigh Cyclopamine-反应细胞系L3.6sl和 Panc 05.04增殖,抑制达 75-80%,且使凋亡率提高2.5到 3.5倍,但是不影响BxPC3-SMOlow 细胞系。[3] Cyclopamine 处理E3LZ10.7细胞系,显著降低Snail mRNA,且提供E-钙粘着蛋白转录。Cyclopamine处理Hedgehog依赖型 L3.6pl 细胞,显著抑制浸润型细胞, 使转移细胞降低500多倍,但是对Hedgehog非依赖性细胞系Panc-1没有影响。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
OS-RC-2 MkfJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHkTWM2OD13Lki2OlYh|ryP MljzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
DOHH-2 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\iXZI1UUN3ME25MlM2Pjh7IN88US=> MkDrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
no-10 MkXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTlwOUCzPUDPxE1? NVjSUIFZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LS-513 NGn3O2hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWDSbYRXUUN3ME2xNU4{PTR5IN88US=> MknHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
ALL-PO MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELRXVlKSzVyPUGxMlc4OzRizszN MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
8-MG-BA NUPvcVd[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrRc|RPUUN3ME2xN{4yOTJ|IN88US=> M4jXTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
RPMI-8402 NHjZbFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\sTWM2OD1zNT64OVM4KM7:TR?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
EoL-1-cell NH;yVIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4O2V2lEPTB;MUiuOVk1QCEQvF2= NV7RW5YzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NALM-6 Mkj4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUf5TmUzUUN3ME2xPU4xOTZ5IN88US=> M2PYflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
DEL NX[3N5dST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nQVWlEPTB;MkCuNVQ4OSEQvF2= NEjCPJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
SR M3jBZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTJ|Lk[3NVUh|ryP MnPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
697 NVjwc2RCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjKcIFKSzVyPUK2MlYyPTVizszN NXntPXpNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
COLO-829 NVjRW5psT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLFcJBXUUN3ME2yOk45PDh|IN88US=> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
EVSA-T MlzTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrNemw4UUN3ME2yO{42PTZzIN88US=> NVHEW4JFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ATN-1 NYLSO2dyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjpTWM2OD1|MT6yN|I6KM7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
L-363 M3zt[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3Pm[WlEPTB;M{GuO|Q3OSEQvF2= Mlm5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LAMA-84 NV23VHpqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXHJR|UxRTN{LkWyNVEh|ryP NWj5clV3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NOS-1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFi4bXBKSzVyPUO0MlI6PTZizszN NFW1OGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
BB30-HNC MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nzW2lEPTB;M{SuN|MxPiEQvF2= M1O1[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
BC-1 MlLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfjfVk5UUN3ME2zO{46PzR4IN88US=> MkP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
IST-SL2 NEXnfFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1HiNWlEPTB;M{iuNlI1KM7:TR?= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
D-392MG NUXrOnNuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTRyLkKyNVUh|ryP MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
no-11 Ml7MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTRyLkW1NlEh|ryP MlXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LC4-1 M4PUOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTRyLki3NVYh|ryP NEjIPFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
A388 NHixcG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTR{LkW4OFgh|ryP MmnPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NTERA-S-cl-D1 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTR{LkewO|Qh|ryP NG\tXJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
CESS NYX4PItGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{H6emlEPTB;NESuNlI{OiEQvF2= NFTCWHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
RS4-11 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWXJR|UxRTR7LkC5N|gh|ryP M1nufFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
MS-1 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\VTWM2OD13MD65N|UyKM7:TR?= NIPtfGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
CTV-1 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTVzLkC3OEDPxE1? NE\vU2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
D-502MG NVu2OYwyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkHZTWM2OD13MT62NlcyKM7:TR?= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ML-2 M33WdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUjJR|UxRTV{LkmxPVUh|ryP MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
SK-NEP-1 NYHz[3lxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3I[2doUUN3ME21N{4{QTJ|IN88US=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
LOXIMVI MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUXVdHZRUUN3ME21N{42QDh2IN88US=> M1LQRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
DJM-1 NGi4VJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYTZSVU3UUN3ME21Ok4{OzlzIN88US=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
GI-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTV4Lk[xOFkh|ryP NHe3NZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
IST-MES1 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LVSmlEPTB;NkCuOVQ6OyEQvF2= NV30NZVIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MV-4-11 NVvjOJR5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITpTWVKSzVyPU[wMlY2OzhizszN Mo[1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
OVCAR-4 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\jeWlEPTB;NkOuOVY2PyEQvF2= M4O1O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KE-37 M2TVT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nVZmlEPTB;Nk[uNlY3QCEQvF2= MljjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
D-542MG MoK3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXod41KSzVyPU[4MlQyOzVizszN NEXZbmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
MHH-PREB-1 NXjTXm1OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULPZ3I6UUN3ME23Nk45PDRzIN88US=> NXXib|dORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MRK-nu-1 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYT3W|B2UUN3ME23N{41PzB3IN88US=> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
D-247MG M{\NXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3q4emlEPTB;N{OuOVQ1OiEQvF2= M1XySVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
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NCI-H69 NF73UGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHCZnJKSzVyPUG0Nk46OzJizszN Mny5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
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KU812 MoD5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3FTWM2OD1zNkGuPVAzKM7:TR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
REH NGDwcIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoLKTWM2OD1zNkKuNVI2KM7:TR?= NEfXcpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
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Becker M1[xfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7ne5lKSzVyPUG3OE4yQCEQvF2= NUXMc4FQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
LB647-SCLC M363bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1npU2lEPTB;MUe1Mlg1PSEQvF2= M17nWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
LU-139 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTF5OD6wNVkh|ryP M4rJXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
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Daudi NFrEVnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjTPHVHUUN3ME2xPVEvOTJ6IN88US=> NHjqR3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
LB996-RCC MmjDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPodIdKSzVyPUG5NU43QTlizszN MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
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MC-CAR NX3oSGE2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVL5fGxZUUN3ME2yNFIvOjV|IN88US=> NYr1ZVBMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
SNB75 NWjXTHJLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\BOHJKSzVyPUKyNU46PCEQvF2= M2TyfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ES4 NYfiUnpDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUHJR|UxRTJ{Mz63PFMh|ryP M4PndVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
KARPAS-422 NGjjN5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXEXG1KSzVyPUKyPE4{PTJizszN NVviU2NuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
NCI-H1648 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4i3WGlEPTB;MkK5MlQ5QSEQvF2= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
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NB1 NYDPeHN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojyTWM2OD1{NkCuOVE3KM7:TR?= NH60PWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
D-336MG NXXVOlVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEXNVXdKSzVyPUK2NE43QThizszN MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BC-3 NVzLRZZMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDa[5V1UUN3ME2yOlUvOTd6IN88US=> MmD6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HCC2218 M3r3eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPiZ402UUN3ME2yOlYvPDF3IN88US=> NFTwUHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
TE-9 NGXjVlVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPtTWM2OD1{Nk[uOlI4KM7:TR?= MkfZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
LB1047-RCC MkDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PIe2lEPTB;Mk[2Mlc2OyEQvF2= M3zhOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
CTB-1 M1nZO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXK0eJNiUUN3ME2yOlkvQTd|IN88US=> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NB7 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTJ5MTFOwG0> NIrLTXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
ST486 NITkNotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPCTnVKSzVyPUK3O{41OTJizszN NEK3O3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
HCC1187 NGPl[IlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVrJR|UxRTJ6Mj64NVEh|ryP MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
NCI-SNU-16 NWjjPFc2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTJ6ND6yOFgh|ryP NHXvTWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
COR-L279 NUnNXIttT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTJ7MT61PFQh|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
ES8 M{jRemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4jyfWlEPTB;Mkm0MlE5OiEQvF2= NV30eoJIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
U-698-M NWK4fWc5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTJ7OD6yOFMh|ryP Mk\TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
HEL Mne0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3HTWM2OD1|MEmuNVQ6KM7:TR?= NGPjbG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
KINGS-1 MnfQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PlXGlEPTB;M{GwMlY4PCEQvF2= NXTOR5o{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KY821 M17vWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXUTnhkUUN3ME2zN|YvPTl3IN88US=> NUHxNVZ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
MZ1-PC M1i2[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXrXIppUUN3ME2zOFUvPjF6IN88US=> NIX2WJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
LS-411N MmH2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTBSG9TUUN3ME2zOVQvPjZizszN MnzuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
SIG-M5 M4DSOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX61WHJUUUN3ME2zOVkvPzh{IN88US=> M{W0N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
HT MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILZfnZKSzVyPUO2O{44OTFizszN NFLDUW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
HC-1 NYLHPXhmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEH5fHRKSzVyPUO2O{44QDdizszN MofvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
NCI-H1694 M4\Gcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mlq0TWM2OD1|N{KuPVM1KM7:TR?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
BB65-RCC MnXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHS4bINKSzVyPUO3Ok4zPDVizszN MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
HAL-01 MlTsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3m0c2lEPTB;M{e5Mlg{QCEQvF2= NVPuSFRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
ARH-77 NYfVfndwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rx[2lEPTB;M{m0MlAxQCEQvF2= Mk[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
MZ7-mel NUDKSGpKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\oSpQ6UUN3ME2zPVcvOjN|IN88US=> M4fRSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SIMA Ml3KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljyTWM2OD12MEOuPVM{KM7:TR?= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
DG-75 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHIe5lGUUN3ME20NVUvPjl6IN88US=> NXL1fFVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
HUTU-80 NVvBW296T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\QNlhNUUN3ME20NVkvOTh3IN88US=> Ml3oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
KNS-42 NXXQbZFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH63UVVKSzVyPUSyOU45OTVizszN M{HRdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
SH-4 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXjGeJZLUUN3ME20NlcvPTZ3IN88US=> Mnz3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlU1OTJ7Lze+V2FPT0WUPD;hQi=>
L-540 NXPkSW9{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\NcmJ7UUN3ME20N|EvODNzIN88US=> NHzQbXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
NB10 MmXXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLVTWM2OD12NEGuNlM1KM7:TR?= M{jUXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
ES1 M2rKNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTR3Mj63OVMh|ryP NULpdHg5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjV2MUK5M{c,W0GQR1XSQE9iRg>?
KMOE-2 NHS5SHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XqfWlEPTB;NEW2MlcyOSEQvF2= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyOVQyOjlxJ{7TRW5ITVJ:L3G+
MC116 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2n5TmlEPTB;NEW4MlEyPiEQvF2= NH7VO2k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPTRzMkmvK|5USU6JRWK8M4E,
RCC10RGB MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITJN4tKSzVyPUS2NE4xODVizszN M{\sTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI2PDF{OT:nQnNCVkeHUkyvZV4>
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neural precursor cells M1m0PWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M1nPeGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2VibnX1doFtKHC{ZXP1dpNweiClZXzsd{BjgSClb3zvcpkh\m:{bXH0bY9vKGG|c3H5MEBGSzVyIE2gNVMvPDRizszNMi=> NUXhSolRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVc3OzFpPkG3OFE4PjNzPD;hQi=>
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U87MG M2C1e2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NUTVOlVwPzJiaILz NYT3PFhlSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVPFdOTyClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRUKGG|c3H5MEBKSzVyIE2gNlIvPSEQvF2u M3qxVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkK2OlU4Lz5{MkKyOlY2PzxxYU6=
A549 NWXISWFoSW62aXPhcoNmeiCjc4PhfS=> NEHqdlNCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{BjgSCPVGOgZZN{[XluIFnDOVAhRSB2OTFOwG0v NYj3UpBTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyNlE5PzJpPkG4NlIyQDd{PD;hQi=>
DU145 M3Wz[mdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIXF[FQ2KHWP M1Hkdlk3KGi{cx?= MmK2S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gSHUyPDViY3XscJMh[XRiNTD1UUBi\nSncjC5OkBpenN? M4XmflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS5NVI2Lz5zOEK0PVEzPTxxYU6=
DU145 M4PXbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFL4VYIyOCC3TR?= NWG3UpM4QTZiaILz MnGwS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gSHUyPDViY3XscJMh[XRiMUCgeW0h[W[2ZYKgPVYhcHK| NXzFelU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFkyOjVpPkG4NlQ6OTJ3PD;hQi=>
HEK293 NFTlbZpHfW6ldHnvckBie3OjeR?= MUHJcohq[mm2aX;uJI9nKGKndHGg[4Ft[WO2b4Pp[IF{\SCrbjDISWszQTNiY3XscJM> NELRb|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S5OFc3Pid-MUe0PVQ4PjZ:L3G+
22Rv MU\GeY5kfGmxbjDhd5NigQ>? Mmi2VoVlfWO2aX;uJI9nKGW6cILld5Nqd25ib3[gVHREUCCvUl7BJIlvKGi3bXHuJFIzWnZiY3XscJM> MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR7NEe2Okc,OTd2OUS3OlY9N2F-
PANC1 NXzxOoJjTnWwY4Tpc44h[XO|YYm= NIG3UogxNjJidV2= M363SVI1KGi{cx?= NGLjNXJKdmirYnn0bY9vKG:oIFjoM2dNUTFvbXXkbYF1\WRiUGTDTEBuWk6DIHX4dJJme3Orb36gbY4hcHWvYX6gVGFPSzFiY3XscJMh[XRiMD6yJJVOKGGodHXyJFI1KGi{czDifUBTXC2SQ2K= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR3MEG3NEc,OjB2NUCxO|A9N2F-
PANC1 NFnZV5NHfW6ldHnvckBie3OjeR?= NXnkOWJCOC52IIXN NWfuU2J7OjRiaILz NUPNSJNCUW6qaXLpeIlwdiCxZjDIbE9IVElzLX3l[IlifGWmIGDUR2ghdVKQQTDlfJBz\XO|aX;uJIlvKGi3bXHuJHBCVkNzIHPlcIx{KGG2IECuOEB2VSCjZoTldkAzPCCqcoOgZpkhWlRvUFPS M2X4TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWwNVcxLz5{MES1NFE4ODxxYU6=
Shh Light2 NX\W[ZozTnWwY4Tpc44h[XO|YYm= MoHXOk4zPSC3TR?= MVqzNEBpenN? M4X5U2lvcGmkaYTpc44hd2ZiTj3wZYxucXSxeXzheIVlKFOqaDDpckBud3W|ZTDTbIghVGmpaISyJINmdGy|IHH0JFYvOjVidV2gZYZ1\XJiM{CgbJJ{KGK7IH\pdoVndHlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5 MlvmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzNUG3N|EoRjF7MUWxO|MyRC:jPh?=
U87MG MVnGeY5kfGmxbjDhd5NigQ>? MWOxNEB2VQ>? MoT0OEBpenN? NVfYSIhkUW6qaXLpeIlwdiCxZjDI[YRo\WixZzDzbYdv[WyrbnegdIF1cHejeTDpckBpfW2jbjDVPFdOTyClZXzsd{Bie3Onc4Pl[EBieyCmb4fuJJJm\3WuYYTpc44hd2ZiR3zpNUBifCBzMDD1UUBi\nSncjC0JIhzeyCkeTDSWE1RS1JiYX7hcJl{cXN? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ{Nk[1O{c,OjJ{Mk[2OVc9N2F-
HEK293 NIHYfllHfW6ldHnvckBie3OjeR?= NYnqVYFOPSC3TR?= MYWxNEBpenN? NHKxR|ZFcXOybHHj[Y1mdnRib3[gRm9FUVC\LXP5Z4xweGGvaX7lJIZzd21iaIXtZY4hW22xIHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYSgOUB2VSCvZXHzeZJm\CCjZoTldkAyOCCqcoOgZpkhTEGSSTDzeIFqdmmwZzDiZZNm\CCobIXvdoV{[2WwY3WgcYlkem:|Y3;wbYMh[XO|YYm= NXy5bIN4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke3N|YxPjNpPkK3O|M3ODZ|PD;hQi=>
HEK293 NF;SWmtHfW6ldHnvckBie3OjeR?= NVTZdnZDPSC3TR?= MmG0NVAhcHK| Mnj6SIl{eGyjY3Xt[Y51KG:oIFLPSGlRYS2leXPsc5BidWmwZTDmdo9uKGi3bXHuJHNudyCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG2IEWgeW0hdWWjc4Xy[YQh[W[2ZYKgNVAhcHK|IHL5JGZCS1NiYX7hcJl{cXN? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd|NkC2N{c,Ojd5M{[wOlM9N2F-
C3H10T1/2 M4DFN2Z2dmO2aX;uJIF{e2G7 NY\lVG4zPTVwNTD0c{A1PTByIH7N M2fqNmNwdXCndHn0bZZmKGmwaHnibZRqd25ib3[gV41wKGmwIH3veZNmKEN|SEGwWFEwOiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHNCTy2rbnT1Z4VlKEeuaUGgeJJidnOlcnnweIlwdmGuIHHjeIl3cXS7IHH0JFU2NjVidH:gOFUxOCCwTTDifUByWEOUIHHuZYx6e2m| MmTnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyN{S1OFEoRjJ|MEe0OVQyRC:jPh?=
U2OS NVWzXVQ3TnWwY4Tpc44h[XO|YYm= NX3IeZFXPSC3TR?= NFvUflE3KGi{cx?= MljERY51[WexbnnzeEBi[3Srdnn0fUBifCClaHnt[ZJq[yCVbX:gOlM{KG23dHHueEBmgHC{ZYPz[YQhcW5iVULPV{Bk\WyuczDjc4V5eHKnc4Ppcoch[mW2YTDhdpJme3SrbkKtS2ZRKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcW62cnHj[YxtfWyjcjDi[ZRiKGG{cnXzeIlvOi2JRmCgZYdoemWpYYTlJIZwem2jdHnvckBifCB3IIXNJIFnfGW{IE[gbJJ{KGK7IHPvcoZw[2GuIH3pZ5Jwe2OxcIm= MojJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkO1NlIoRjJ|ME[zOVIzRC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Snail / E-cadherin / Slug / Vimentin ; 

PubMed: 26859575     


SW1736 and WRO82 cells were incubated in the presence of vehicle (0.5% DMSO) or the indicated concentrations of cyclopamine or GANT61 for 72 hr. The cells were harvested and analyzed for the expression of several genes involved in EMT.

Gli1 / TGF-β1 / CXCR4; 

PubMed: 26137001     


Western blotting demonstrated that Gli1, TGF-β1 and CXCR4 protein expression levels were downregulated in the AGS cells treated with cyclopamine for 24 h.

NF-κB / Cyclin D1 / MMP2 / MMP9; 

PubMed: 23599805     


Whole cell lysates were prepared, and 50 μg proteins were resolved using SDS-PAGE, followed by immunoblotting with the indicated specific antibodies against NF-κB, cyclin D1, MMP2 and MMP9. 

26859575 26137001 23599805
Immunofluorescence
PCNA / β-catenin; 

PubMed: 28747625     


Immunofluorescence images demonstrating the distribution of PCNA or β-catenin in EH cells pre-treated with cyclopamine in presence and absence of estrogen for 24 h. Experiments were repeated at least three times.

Vimentin; 

PubMed: 24553082     


Vimentin protein expression was reduced following treatment with cyclopamine (20 μmol/L; 72 h), detected by immunocytochemistry.

28747625 24553082
体内研究 Cyclopamine 每天按50 mg/kg剂量处理小鼠,持续22天,消除HUCCT1移植瘤,且没有明显副作用。[2] Cyclopamine 按1.2 mg剂量处理Panc 05.04和 L3.6sl驱动的肿瘤7天,前者诱导明显的肿瘤细胞凋亡,后者降低肿瘤质量,降低达50-60%,但是作用于BxPC3-SMOlow肿瘤没有效果。[3] Cyclopamine单独处理E3LZ10.7 和L3.6pl移植瘤,显著抑制肿瘤转移,且和 Gemcitabine联用处理,完全废除转移。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

激酶实验:

Gli-Luc实验测定Hh 信号通路末期, 即使用荧光素酶进行读数,测定Gli的转录调控。Cyclopamine 在DMSO中连续稀释用于实验,然后加到空的实验板上。TM3Hh12 细胞(TM3 细胞含Hh反应受体基因结构 pTA-8xGli-Luc) 悬浮在含5% FBS 和15 mM Hepes pH 7.3的F12 Ham’s/DMEM (1:1)培养基上, 加到实验板上,与Cyclopamine在37oC下温育30分钟左右。1 nM Hh-Ag 1.5 加到实验板上,然后在37oC下温育。48小时后,在实验板中加入Bright-Glo或MTS,然后在492 nm处测定发光度或吸光度。通过分析反应曲线,使用R统计软件包测定IC50值。
细胞实验:[2]
- 合并
  • Cell lines: SEG1, OE33, KYAE, KYSE180, SNU1, AGS, SNU16, NCI-N-87, HUCCT1, PANC1, PL5, PL6, BXPC3, HS766T, KYSE150, GBD1, DLD1, 和HCT116
  • Concentrations: 溶于DMSO, 终浓度为3 μM
  • Incubation Time: 4天
  • Method: 在96孔板上,用Cyclopamine 处理细胞。通过MTS实验测定细胞活力。进行CellTiter96比色测定,进行2和4天,然后在490 nm处通过测定光密度而测定存活细胞。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: 皮下注射HUCCT1细胞的无胸腺裸鼠
  • Dosages: 50 mg/kg/day
  • Administration: 皮下注射处理
    (Only for Reference)

溶解度 (25°C)

体外 DMF 10 mg/mL warmed (24.29 mM)
Ethanol 2 mg/mL warmed (4.85 mM)
DMSO Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 411.62
化学式

C27H41NO2

CAS号 4449-51-8
储存条件 粉状
溶于溶剂
别名 11-deoxojervine

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How to reconstitute the compound for in vivo use in mice?

  • 回答:

    One paper dissolved this drug in DMSO, and diluted in saline: Berman DM, et al. Nature, 2003, 425(6960), 846-851. Alternatively, you can try this vehicle: 10% DMSO+30% PEG 300+5% Tween 80+ddH2O for P.O. When preparing the solution, please dissolve the compound in DMSO clearly first. Then add PEG300 and Tween, after they mixed well, dilute with water.

Hedgehog/Smoothened Signaling Pathway Map

Hedgehog/Smoothened Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID