NVP-AEW541

For research use only. Not for use in humans.

目录号:S1034 别名: AEW541

NVP-AEW541 Chemical Structure

CAS No. 475489-16-8

NVP-AEW541是一种有效的IGF-1R/InsR抑制剂,在无细胞试验中IC50为150 nM/140 nM,在细胞试验中对IGF-1R具有较高的作用和选择性。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 4890.3 现货
RMB 1711.38 现货
RMB 4316.72 现货
RMB 8657.46 现货
RMB 43055.62 现货
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客户使用Selleck生产的NVP-AEW541发表文献56篇:

产品安全说明书

IGF-1R抑制剂选择性比较

生物活性

产品描述 NVP-AEW541是一种有效的IGF-1R/InsR抑制剂,在无细胞试验中IC50为150 nM/140 nM,在细胞试验中对IGF-1R具有较高的作用和选择性。
靶点
Insulin Receptor [1]
(Cell-free assay)
IGF-1R [1]
(Cell-free assay)
FLT3 [1]
(Cell-free assay)
Tek [1]
(Cell-free assay)
FLT1 [1]
(Cell-free assay)
0.14 μM 0.15 μM 0.42 μM 0.53 μM 0.6 μM
体外研究

在纯化的激酶/重组激酶域实验中,NVP-AEW541也抑制InsR, Tek, Flt1 和 Flt3,IC50分别为140 nM, 530 nM, 600 nM 和 420 nM。在细胞水平,NVP-AEW541选择性更高,比InsR选择性高27倍。NVP-AEW541抑制IGF-I调节的生存,软琼脂,和MCF-7细胞增殖,IC50分别为0.162 μM, 0.105 μM 和 1.64 μM。NVP-AEW541作用于NWT-21细胞,也降低 p-IGF-IR 和 p-PKB 水平。[1] NVP-AEW541 作用于培养在低血清培养基和含10% FBS的培养基中的TC-71肌肉骨骼肉瘤细胞,抑制生长。NVP-AEW541作用于肉瘤细胞系(TC-71, SK-N-MC, SaoS-2, RD/18 和 RH4),抑制细胞周期进展和诱导细胞周期在G1期停顿。 [2]NVP-AEW541可抑制神经母细胞瘤细胞生长,IC50为0.4-6.8 μM。在这些细胞中可以检测到亚二倍体片段增多及 S和 G2-M 期细胞消耗。在神经母细胞瘤细胞中,NVP-AEW541驱动的 IGF-IR 受抑制,可降低 Akt磷酸化,而不是Erk1和Erk2磷酸化。[3] NVP-AEW541 抑制神经胶质瘤细胞生长,且破坏HIF1α 稳定化启动的自分泌环。[4]最新研究显示NVP-AEW541抑制 PC3, DU145, 和22Rv1 前列腺癌细胞增殖和活性。NVP-AEW541 作用于22Rv1和 DU415 细胞而不是PC3细胞,降低 p-Akt水平,不会影响整体Akt水平,说明PTEN状态可决定NVP-AEW541的有效性。NVP-AEW541诱导的放射敏感度决定于Akt 激活状态。NVP-AEW541作用于PC3, DU145, 和 22Rv1细胞,可提高H2AX 磷酸化。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NWT-21 NYrTU3FHU2mwYYPlJIF{e2G7 NWjl[4lwhjFy4pEK{txO M4TGdGROW09? NH3ORZRqdmirYnn0d{BKT0ZvSWKge4l1cCCLQ{WwJI9nKDBwMEi2JOKyKDBwMEK4JO69VQ>? NIjESos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUC1NFkyPSd-MUWwOVA6OTV:L3G+
A14 NVzCZ4JSU2mwYYPlJIF{e2G7 MWL+NVDjiIsQvF2= MVPEUXNQ MnTpbY5pcWKrdIOgTY5{WiC5aYToJGlEPTBib3[gNk4{KMLzIECuNVY{KM7:TR?= NUTJUXdwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWwOVA6OTVpPkG1NFUxQTF3PD;hQi=>
A431  NWXZZllRU2mwYYPlJIF{e2G7 NF;VTlB,OTEkgJtOwG0> NYfyVoxjTE2VTx?= M{TvfolvcGmkaYTzJGhGWjFid3n0bEBKSzVyIH;mJF4yOCEQvF2= M3zGc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MEWwPVE2Lz5zNUC1NFkyPTxxYU6=
A31  NX7XRmNDU2mwYYPlJIF{e2G7 M1fscJ4yOOLCit88US=> NYfDU3F4TE2VTx?= NVLnbnlXcW6qaXLpeJMhWESJRmKge4l1cCCLQ{WwJI9nKD5zMDFOwG0> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTB3MEmxOUc,OTVyNUC5NVU9N2F-
GIST882 NVvmWVE{U2mwYYPlJIF{e2G7 NFO2b|d,OTEkgJtOwG0> M4G2UWROW09? NHXwWY9qdmirYnn0d{BkNUurdDD3bZRpKEmFNUCgc4YhRjVizszN Mk\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTVyNUC5NVUoRjF3MEWwPVE2RC:jPh?=
32D-Bcr-Abl M{PHWGtqdmG|ZTDhd5NigQ>? MUD+NVDjiIsQvF2= M3\6fmROW09? MWXpcohq[mm2czDCZ5IuSWKuIICyNVAhf2m2aDDJR|UxKG:oIE6xNEDPxE1? NHfyToQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUC1NFkyPSd-MUWwOVA6OTV:L3G+
MCF-7  MlHZR5l1d3irY3n0fUBie3OjeR?= NFOzS3RFVVOR NVLMU|NmUUN3ME2xMlY1KM7:TR?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTB3MEmxOUc,OTVyNUC5NVU9N2F-
NWT-21 NWH4OFFpT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= MkTmSG1UVw>? NVHGVHJRUUN3ME2wMlE3OyEQvF2= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTB3MEmxOUc,OTVyNUC5NVU9N2F-
TC-71 Ml;qS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? MVL+NUDPxE1? MVPEUXNQ M3frcolvcGmkaYTzJIlve3WuaX6tcIls\SCpcn;3eIgh\mGldH;yMWnjiJOvZXTpZZRm\CCpcn;3eIg> MoPlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV6NkezPFYoRjF3OE[3N|g3RC:jPh?=
TC-71 MYDHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? MmPNglfjiIsQvF2= MX7EUXNQ MYDJR|UxRDBwNTFOwG0> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTh4N{O4Okc,OTV6NkezPFY9N2F-
Saos-2 NITidmdIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MWX+O-KBks7:TR?= NEi1b4FFVVOR M{nSbWlEPTB:MzFOwG0> M3PrSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OE[3N|g3Lz5zNUi2O|M5PjxxYU6=
U-2OS M4DDU2dzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NVXHU2V2hjgkgJtOwG0> M3rye2ROW09? MUTJR|UxRDBwNTFOwG0> M2nvOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OE[3N|g3Lz5zNUi2O|M5PjxxYU6=
SK-ES-1 MkHzS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? Mlq2glfjiIsQvF2= NYP0bYlZTE2VTx?= M{jtXGlEPTB:MD61JO69VQ>? NYSzV5F1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW4Olc{QDZpPkG1PFY4Ozh4PD;hQi=>
SK-N-MC M3Xvemdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NETHbIJ,P+LCit88US=> NXP5TndGTE2VTx?= M33QPWlEPTB:MD61JO69VQ>? NI\iXIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUi2O|M5Pid-MUW4Olc{QDZ:L3G+
RD-ES M165W2dzd3e2aDDpcohq[mm2b4L5JIF{e2G7 Ml3DglfjiIsQvF2= MoXPSG1UVw>? NUf2UFBLUUN3MEywMlUh|ryP NH3Qb3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUi2O|M5Pid-MUW4Olc{QDZ:L3G+
SJ-Rh 30 NWSye|lUT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= Mn6xglfjiIsQvF2= NWDO[4F5TE2VTx?= MXvJR|UxRDBwNTFOwG0> M4rSclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OE[3N|g3Lz5zNUi2O|M5PjxxYU6=
SJ-Rh 4 NU\mR3NUT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= Mn;QglfjiIsQvF2= NVTZXZFrTE2VTx?= NU[0UFVGUUN3MEywMlUh|ryP MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTh4N{O4Okc,OTV6NkezPFY9N2F-
6647 MnPxS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? M4TlTp446oDMzszN NGjVXG5FVVOR M2nYRWlEPTB:MD61JO69VQ>? MkfnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV6NkezPFYoRjF3OE[3N|g3RC:jPh?=
SARG NFPOPVBIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M1;CTp446oDMzszN MmDpSG1UVw>? MULJR|UxRDNizszN Mo\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV6NkezPFYoRjF3OE[3N|g3RC:jPh?=
MOS NVrWO41wT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NUHX[JRphjgkgJtOwG0> M2SzUmROW09? NYDOS5lLUUN3MEy0JO69VQ>? MnG5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV6NkezPFYoRjF3OE[3N|g3RC:jPh?=
IOR/OS7 MlSzS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NEHLOFR,P+LCit88US=> Mn76SG1UVw>? MoDsTWM2ODxzIN88US=> M1nkT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OE[3N|g3Lz5zNUi2O|M5PjxxYU6=
IOR/OS9 NETiUndIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MVL+O-KBks7:TR?= NGPPU5hFVVOR MmK1TWM2ODx4IN88US=> NH;kUmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUi2O|M5Pid-MUW4Olc{QDZ:L3G+
IOR/OS10 MmSwS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? M{j5[p446oDMzszN NVXLbIh6TE2VTx?= NVP4Z2dnUUN3MEy1JO69VQ>? MnrOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV6NkezPFYoRjF3OE[3N|g3RC:jPh?=
IOR/OS14 MnnTS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NXPSVlF1hjgkgJtOwG0> NWPrXlNWTE2VTx?= M2Tsb2lEPTB:NDFOwG0> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTh4N{O4Okc,OTV6NkezPFY9N2F-
LAP35 M3u0O2dzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NFnmRnF,P+LCit88US=> M2HufGROW09? Mnf3TWM2ODxyLkWg{txO M2faUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OE[3N|g3Lz5zNUi2O|M5PjxxYU6=
IOR/BRZ NYTBbW5MT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= MVr+O-KBks7:TR?= NHnDdodFVVOR MkHrTWM2ODxyLkWg{txO NFW2TJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUi2O|M5Pid-MUW4Olc{QDZ:L3G+
IOR/CAR NWiwOXl[T3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= Mk\JglfjiIsQvF2= NV3PU2VsTE2VTx?= Mmn3TWM2ODxzIN88US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTh4N{O4Okc,OTV6NkezPFY9N2F-
IOR/NGR Mly2S5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NXy5fld5hjgkgJtOwG0> MWrEUXNQ NF;KT|ZKSzVyPECuOUDPxE1? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTh4N{O4Okc,OTV6NkezPFY9N2F-
IOR/RCH NYnRb|lCT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= MmTRglfjiIsQvF2= MVvEUXNQ NWH1W|FlUUN3MEywMlUh|ryP M1HnblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OE[3N|g3Lz5zNUi2O|M5PjxxYU6=
RMZ-RC2 MX\Hdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? MmjMglfjiIsQvF2= MYjEUXNQ NYK4UJprUUN3MEywMlUh|ryP M4n2UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3OE[3N|g3Lz5zNUi2O|M5PjxxYU6=
CCA NF7Gc3ZIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M3TIXJ446oDMzszN MUXEUXNQ MXzJR|UxRDJizszN NWi0NolQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW4Olc{QDZpPkG1PFY4Ozh4PD;hQi=>
RD/18 NXO3OpA5T3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= MoDGglfjiIsQvF2= NGHy[|dFVVOR M1fsSmlEPTB:NDFOwG0> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTh4N{O4Okc,OTV6NkezPFY9N2F-
OVCAR-3 M2T2emdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NFXPe3d,OTYkgJtOwG0> MnXuSG1UVw>? NVjuNoppcW6qaXLpeJMh[2WubDDwdo9tcW[ncnH0bY9v NWr5NY5PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zNFA5OjBpPkG2N|AxQDJyPD;hQi=>
OVCAR-4 MWnHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? NVnXbINNhjF34pEK{txO NGH2VoxFVVOR MmHxbY5pcWKrdIOgZ4VtdCCycn;sbYZmemG2aX;u NU\KSnlwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zNFA5OjBpPkG2N|AxQDJyPD;hQi=>
OVCAR-3 Ml\6RZBweHSxc3nzJIF{e2G7 MXf+NVXjiIsQvF2= NWXNNWFzTE2VTx?= NW\lR2FXcW6mdXPld{BieG:ydH;zbZM> MkflQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ|MEC4NlAoRjF4M{CwPFIxRC:jPh?=
OVCAR-4 MlTjRZBweHSxc3nzJIF{e2G7 MWP+NVXjiIsQvF2= NFv2RVJFVVOR NIT3[YlqdmS3Y3XzJIFxd3C2b4Ppdy=> NX\5Zo9GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[zNFA5OjBpPkG2N|AxQDJyPD;hQi=>
OVCAR-3 M2PtSGZ2dmO2aX;uJIF{e2G7 M4\ZO54yPeLCit88US=> Ml3vSG1UVw>? NYHBcXpOTGWlcnXhd4V{KHCqb4PwbI9zgWyjdHnvckBw\iCDS2S= M4DjVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4M{CwPFIxLz5zNkOwNFgzODxxYU6=
Huh-7 NGPTN3RIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NEC0TJJ,OTEkgJtOwG0> MWLEUXNQ M2m0cGlEPTB;MT60JO69VQ>? NGrYc4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkWzNFc{PCd-MU[1N|A4OzR:L3G+
Hep-G2 NYjUbmhMT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NFjxWlV,OTEkgJtOwG0> MXjEUXNQ MU\JR|UxRTFwODFOwG0> M3G2PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUOwO|M1Lz5zNkWzNFc{PDxxYU6=
Hep-3B M3TGbWdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 MX3+NVDjiIsQvF2= MUPEUXNQ MV7JR|UxRTFwOTFOwG0> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjV|MEezOEc,OTZ3M{C3N|Q9N2F-
SK-Hep-1 MVPHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? MYn+NVDjiIsQvF2= NXrUZlJCTE2VTx?= NIH1U4FKSzVyPU[uPUDPxE1? NIrUeoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkWzNFc{PCd-MU[1N|A4OzR:L3G+
Huh-7 M2XwUWZ2dmO2aX;uJIF{e2G7 NGL1dm5,OTEkgJtOwG0> MkPWSG1UVw>? MoK5TY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dB?= MoDMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ3M{C3N|QoRjF4NUOwO|M1RC:jPh?=
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SK-Hep-1 MVPGeY5kfGmxbjDhd5NigQ>? MmPPglEx6oDMzszN M1HURWROW09? MkSyTY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dB?= NY[1NVBuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[1N|A4OzRpPkG2OVMxPzN2PD;hQi=>
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primary colorectal cancer cells MVzGeY5kfGmxbjDhd5NigQ>? MYn+OgKBks7:TR?= NVjBeWdTTE2VTx?= NVrGbWZw[Wy2ZYLzJJRp\SCvb4LwbI9td2e7IH;mJJRp\SC{ZX3hbY5qdmdiY3XscJM> M{HVR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MEC3NFE2Lz5zN{CwO|AyPTxxYU6=
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SK-N-BE MWjGeY5kfGmxbjDhd5NigQ>? M2DJWp45KM7:TR?= M2LlT2ROW09? MXzpcohq[mm2czDJS2YuUUlvbXXkbYF1\WRic4TpcZVt[XSrb36gc4YhUUeILVnSJIFv\CCDa4S= NYfrVFhwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlE5QThpPkG3NVIyQDl6PD;hQi=>
LAN-5 Mlz2SpVv[3Srb36gZZN{[Xl? NGXUZ3B,QCEQvF2= M1\SW2ROW09? M2[ycolvcGmkaYTzJGlITi2LST3t[YRq[XSnZDDzeIlufWyjdHnvckBw\iCLR1[tTXIh[W6mIFHreC=> NHTXbYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{GyNVg6QCd-MUexNlE5QTh:L3G+
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SH-EP NInjWoxHfW6ldHnvckBie3OjeR?= NFvUbo9,QCEQvF2= MoO0SG1UVw>? NF3nbZlqdmirYnn0d{BKT0ZvSVmtcYVlcWG2ZXSgd5RqdXWuYYTpc44hd2ZiSVfGMWlTKGGwZDDBb5Q> M4O4PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUKxPFk5Lz5zN{GyNVg6QDxxYU6=
SK-N-AS NHf3SXJHfW6ldHnvckBie3OjeR?= MUT+PEDPxE1? MmXuSG1UVw>? NVrxRoVtcW6qaXLpeJMhUUeILVnJMY1m\GmjdHXkJJN1cW23bHH0bY9vKG:oIFnHSk1KWiCjbnSgRYt1 M4\2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUKxPFk5Lz5zN{GyNVg6QDxxYU6=
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SY-5Y(N) Mlj6SpVv[3Srb36gZZN{[Xl? MYL+PEDPxE1? NGnEbJRFVVOR Mk\ybY5pcWKrdIOgTWdHNUmLLX3l[IlifGWmIIP0bY12dGG2aX;uJI9nKEmJRj3JVkBidmRiQXv0 MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{MUi5PEc,OTdzMkG4PVg9N2F-
GI-CA-N NH3jfmFIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MmHWglgh|ryP MVjEUXNQ NX\kcIQ5UUN3ME2gOk45KM7:TR?= NFfncGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{GyNVg6QCd-MUexNlE5QTh:L3G+
SH-EP NFzmdHRIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MlvDglgh|ryP MVrEUXNQ NYrkWYlWUUN3ME2gN{DPxE1? M3TpTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5MUKxPFk5Lz5zN{GyNVg6QDxxYU6=
HTLA-230 MkS1S5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? MXf+PEDPxE1? MVnEUXNQ NIrjV41KSzVyPTCwMlUh|ryP NHHJXlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{GyNVg6QCd-MUexNlE5QTh:L3G+
SK-N-BE2c NFTYVFVIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NU\vW2FIhjhizszN M3[5TWROW09? MVjJR|UxRSBzLkGg{txO Ml\ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzMkG4PVgoRjF5MUKxPFk5RC:jPh?=
SK-N-BE2 NESze|NIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NVjVcGdthjhizszN NWjhW|R{TE2VTx?= M1j4dmlEPTB;IEOg{txO MlnqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzMkG4PVgoRjF5MUKxPFk5RC:jPh?=
SY-5Y (N) NILaU25Iem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NETsSId,QCEQvF2= NVTzW4xHTE2VTx?= NH3iNIxKSzVyPTCyMlQh|ryP MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{MUi5PEc,OTdzMkG4PVg9N2F-
LAN-5 MkLqS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NITiXol,QCEQvF2= MmrMSG1UVw>? NFLj[5BKSzVyPTCwMlQh|ryP NEDVNI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{GyNVg6QCd-MUexNlE5QTh:L3G+
KCNR NHPseYhIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NVvJcIIyhjhizszN NYLLcGZQTE2VTx?= NV;hbGZ5UUN3ME2gNE41KM7:TR?= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{MUi5PEc,OTdzMkG4PVg9N2F-
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SK-N-AS NXf5XG9mT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NGHub|d,QCEQvF2= NWHDe|F[TE2VTx?= MlPkbY5lfWOnczDhdI9xfG:|aYO= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{MUi5PEc,OTdzMkG4PVg9N2F-
KCNR NI\ZZ2lCeG:ydH;zbZMh[XO|YYm= NUjIRnFqhjhizszN MULEUXNQ Ml\HbY5lfWOnczDhdI9xfG:|aYO= NWO2T4M6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlE5QThpPkG3NVIyQDl6PD;hQi=>
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HTLA-230 MVnBdI9xfG:|aYOgZZN{[Xl? Mn\Dglgh|ryP NHjXWYVFVVOR MoXubY5lfWOnczDhdI9xfG:|aYO= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{MUi5PEc,OTdzMkG4PVg9N2F-
SK-N-BE2c NUHkb2Z1SXCxcITvd4l{KGG|c3H5 NHXiR4h,QCEQvF2= MWPEUXNQ MYnpcoR2[2W|IHHwc5B1d3Orcx?= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF{MUi5PEc,OTdzMkG4PVg9N2F-
SY-5Y (N) MVTBdI9xfG:|aYOgZZN{[Xl? MkPsglgh|ryP NFy2OoZFVVOR NW[x[ldlcW6mdXPld{BieG:ydH;zbZM> NEe1dmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{GyNVg6QCd-MUexNlE5QTh:L3G+
HL60AR NI\Tb4NHfW6ldHnvckBie3OjeR?= NIfybYUyPjBibl2= Ml3V[Y5p[W6lZYOgeIhmKGyndnXsd{Bw\iCyMkfLbZAy NWDTcWdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezOlEzOjVpPkG3N|YyOjJ3PD;hQi=>
HL60AR MYjBdI9xfG:|aYOgZZN{[Xl? MkG0glIxOCCwTR?= M3nQZYlv\HWlZYOgZZBweHSxc3nz NXn5OnFvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezOlEzOjVpPkG3N|YyOjJ3PD;hQi=>
HPAF-II MY\LbY5ie2ViYYPzZZk> MnSwglEh|ryP M4j2ZmROW09? NXHKc4tjcW6qaXLpeJMhUUeILVmtcYVlcWG2ZXSgd4lodmGubHnu[y=> MonCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2NEW1NlAoRjF6NES1OVIxRC:jPh?=
HPAF-II MVPHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? MVL+NkDPxE1? NE\W[m1FVVOR MlTlbY5pcWKrdIOgZ4VtdCCycn;sbYZmemG2aX;u M2D0VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NES1OVIxLz5zOES0OVUzODxxYU6=
HPAF-II MlH5SpVv[3Srb36gZZN{[Xl? NVPSd3lqhjJizszN M1nZXWROW09? NUHETJJPcW6qaXLpeJMh[mG|YXygZY5lKEmJRj3JMY1m\GmjdHXkJJBidmO{ZXH0bYMh[2GwY3XyJINmdGxibXnndoF1cW:w MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR2NUWyNEc,OTh2NEW1NlA9N2F-
TFK-1 M1jKbGdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 MmqzglI2OCCwTR?= MV7EUXNQ MYTJR|UxRTBwMk[g{txO MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB4NkezOEc,OjByNk[3N|Q9N2F-
EGI-1 Mn7HS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NVrjXJh1hjJ3MDDuUS=> M2TKc2ROW09? NUXXU4JvUUN3ME2wMlI5KM7:TR?= NG\xbFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC2Olc{PCd-MkCwOlY4OzR:L3G+
CC-LP-1 MYXHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? NWPDOZN{hjJ3MDDuUS=> MX7EUXNQ NEjISY9KSzVyPUCuNVUh|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB4NkezOEc,OjByNk[3N|Q9N2F-
CC-SW-1 MV\Hdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? M3nYd54zPTBibl2= Mn;ISG1UVw>? M2TvZWlEPTB;MD61OEDPxE1? M{jJ[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyME[2O|M1Lz5{MEC2Olc{PDxxYU6=
Sk-ChA-1 NF3zRYlIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NW\ybJZThjJ3MDDuUS=> NHe4elNFVVOR NH;x[m5KSzVyPUCuNkDPxE1? NIT4VXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC2Olc{PCd-MkCwOlY4OzR:L3G+
Mz-ChA-1 NHvsUWhIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NVHDe|VNhjJ3MDDuUS=> NVfqR3Z[TE2VTx?= NXLWRpJCUUN3ME2xMlM6KM7:TR?= NG\WZm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC2Olc{PCd-MkCwOlY4OzR:L3G+
Mz-ChA-2 NEP0WYtIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MmrHglI2OCCwTR?= NFyzU5VFVVOR NV3uXIZpUUN3ME2wMlc{KM7:TR?= NF7QfG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC2Olc{PCd-MkCwOlY4OzR:L3G+
ECC-1 NVrG[ZV1U2mwYYPlJIF{e2G7 M2X5VZ4yOCEQvF2= MWXEUXNQ Mnm3bY5pcWKrdIOgTWdHNUmUIHHjeIl3[XSrb36gZpkhQThn NGriV|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5OVM{PSd-MkGyPVU{OzV:L3G+
Ishikawa NVrTR3FQU2mwYYPlJIF{e2G7 M4G5XJ4yOCEQvF2= MmG1SG1UVw>? NYTaOXl4cW6qaXLpeJMhUUeILVnSJIFkfGm4YYTpc44h[nliOUOl NEXrRVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5OVM{PSd-MkGyPVU{OzV:L3G+
USPC-1 MYDLbY5ie2ViYYPzZZk> M1;rSJ4yOCEQvF2= MVrEUXNQ MoT0bY5pcWKrdIOgTWdHNUmUIHHjeIl3[XSrb36gZpkhOTByJR?= NY\RN|lPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVU{OzVpPkKxNlk2OzN3PD;hQi=>
USPC-2 NYD2dI9CU2mwYYPlJIF{e2G7 NFnFOW5,OTBizszN M1Hnb2ROW09? NWq3NVFwcW6qaXLpeJMhUUeILVnSJIFkfGm4YYTpc44h[nliOU[l NVPhc5NuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVU{OzVpPkKxNlk2OzN3PD;hQi=>
ECC-1 Mm\yS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NEHydZh,OTBizszN MWrEUXNQ MnXw[IVkemWjc3XzJINmdGxicILvcIln\XKjdHnvci=> M{HHTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkm1N|M2Lz5{MUK5OVM{PTxxYU6=
Ishikawa NIPsV|VIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= MmK3glExKM7:TR?= MXPEUXNQ M1voRYRm[3KnYYPld{Bk\WyuIIDyc4xq\mW{YYTpc44> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7NUOzOUc,OjF{OUWzN|U9N2F-
USPC-1 MlHQS5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? NXLHOodHhjFyIN88US=> NV\0b2hKTE2VTx?= NUjDb2xy\GWlcnXhd4V{KGOnbHygdJJwdGmoZYLheIlwdg>? Ml\2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUWzN|UoRjJzMkm1N|M2RC:jPh?=
USPC-2 MXTHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? MX\+NVAh|ryP MlP4SG1UVw>? Mmj1[IVkemWjc3XzJINmdGxicILvcIln\XKjdHnvci=> NV3SfFFmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVU{OzVpPkKxNlk2OzN3PD;hQi=>
Ba/F3 M4ntdGZ2dmO2aX;uJIF{e2G7 MlvPTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDJS2YuOSC{ZXPldJRweiBqdX7rco94diCxcnnnbY4qKHS{YX7z[oVkfGWmIHnuJGJiN0Z|IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwheHKxbHnm[ZJifGmxbjygTWM2OD1yLkCy{txO MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl3MUe1N{c,OjZ7NUG3OVM9N2F-
HEK293 MnnNSpVv[3Srb36gZZN{[Xl? MnH0OlAhdWmwcx?= MoG1TY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDJS2YuOSC{ZXPldJRweiBqdX7rco94diCxcnnnbY4qKGG3dH;wbI9{eGixconsZZRqd25idILhcpNn\WO2ZXSgbY4hUEWNMkmzJINmdGy|IIDy[ZRz\WG2ZXSg[o9zKDZyIH3pcpMh\m:ubH;3[YQh[nliSVfGMVEhe3SrbYXsZZRqd25ibXXhd5Vz\WRiYX\0[ZIhOTBibXnud{BjgSCzdXHueIl1[XSrdnWgW4V{fGW{bjDicI91KGGwYXz5d4l{NCCLQ{WwQVAvODZ3zszN M2HHbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUWxO|U{Lz5{Nkm1NVc2OzxxYU6=
HEK293 Mlr4SpVv[3Srb36gZZN{[Xl? NV\TfVFpTGm|cHzhZ4Vu\W62IH;mJHs{UF1vZH;m[ZRqdGmmZTDmdo9uKGi3bXHuJGVTTyClaHHucoVtKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMtKEmFNUC9NE4yO87:TR?= Mn7NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7NUG3OVMoRjJ4OUWxO|U{RC:jPh?=
Ba/F3 MofGSpVv[3Srb36gZZN{[Xl? M2nxUGlvcGmkaYTpc44hd2ZiZoXscEBt\W6pdHigbY5{fWyrbjDy[YNmeHSxcjCoeY5sdm:5bjDvdolocW5rIITyZY5{\mWldHXkJIlvKEKjL1[zJINmdGy|IHHzd4V{e2WmIHHzJINmdGxicILvcIln\XKjdHnvckwhUUN3ME2wMlI1PM7:TR?= NYLZRY9zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5OVE4PTNpPkK2PVUyPzV|PD;hQi=>
HEK293 NEXUOYdHfW6ldHnvckBie3OjeR?= MnPEOlAhdWmwcx?= NYC2V|lmUW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBqdnO3bHnuJJJm[2WydH;yJEh2dmuwb4fuJI9zcWerbjmgZZV1d3Cqb4PwbI9zgWyjdHnvckB1emGwc3\lZ5Rm\CCrbjDISWszQTNiY3XscJMheHKndILlZZRm\CCob4KgOlAhdWmwczDmc4xtd3enZDDifUBKT0ZvMTDzeIlufWyjdHnvckBu\WG|dYLl[EBi\nSncjCxNEBucW6|IHL5JJF2[W62aYTheIl3\SCZZYP0[ZJvKGKub4SgZY5idHm|aYOsJGlEPTB;MD64PVLPxE1? M1;CZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUWxO|U{Lz5{Nkm1NVc2OzxxYU6=
TC32 NEnzW3ZyUFSVIHHzd4F6 M3zLZ5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCWQ{OyJINmdGy| MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB-EBc1 MW\xTHRUKGG|c3H5 NX[wc25UeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CMWVD[zFiY3XscJM> MnXxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Saos-2 MXnxTHRUKGG|c3H5 NXXhbopTeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPhc5MuOiClZXzsdy=> M2HMOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5 NV7PPIoxeUiWUzDhd5NigQ>? NFywPHFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO= M3LTbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 MlvSdWhVWyCjc4PhfS=> M3qxS5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCRSGOtOVAh[2WubIO= M4\6VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD MoTGdWhVWyCjc4PhfS=> MVPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 NHvuS4RyUFSVIHHzd4F6 NH;sRmRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUni0NUBk\Wyucx?= NGPUN2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+

... Click to View More Cell Line Experimental Data

体内研究 NVP-AEW541(50 mg/kg, 口服处理)作用于NWT-21移植瘤,导致基础型和IGF-I诱导型受体的废除,也抑制 PKB和MAPK磷酸化,T/C 值为14%。[1]NVP-AEW541(50 mg/kg) 作用于HTLA-230和 SK-N-BE2c移植瘤,引起肿瘤缩小,没有全身毒性迹象。NVP-AEW541作用于Matrigel包被的细胞和HTLA-230移植瘤,可以抑制肿瘤入侵。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

体外酶法测定:

NVP-AEW541溶解在DMSO(10 mM) 中,储存在-20oC下。稀释液在1:1的DMSO/水中现配。在酶法测定中DMSO的最终浓度<0.5 %。蛋白激酶实验在96孔板上进行,加入20 μl 125 mM EDTA终止反应。随后, 30 μl (c-Abl, c-Src, IGF-1R) 反应混合物转移到Immobilon-P转印膜上,加入甲醇预浸泡5分钟,用水冲洗, 然后加入0.5 % H3PO4浸泡5分钟,然后装在真空管中。识别所有样本后,用200 μl 0.5 % H3PO4冲洗每孔。分离膜,在搅拌器上用1.0 % H3PO4冲洗4次,其中一次加入乙醇。烘干后,建立Packard TopCount 96孔框架,每孔加入10 μl Microscint, 膜计数。通过线性回归分析NVP-AEW541在四种不同浓度(0.01, 0.1, 1, 和10 μM)下的抑制百分数来计算IC50值。37oC下,每分钟,每毫克蛋白中,[γ33P]ATP转化到底物蛋白中的1 nM 33P表示蛋白激酶活性。
细胞实验:[1]
- 合并
  • Cell lines: MCF-7细胞
  • Concentrations: 30到300 nM
  • Incubation Time: 30分钟
  • Method: NVP-AEW541直接加到琼脂培养基中,最终浓度为30到300 nM。MCF7生长培养基中下层包括每孔0.5 ml 细菌琼脂。包被培养板,储存在孵育器中(37oC, 5% CO2)至少30分钟,固定培养基,然后加入上层琼脂。在生长培养基的0.5ml上层0.4%琼脂中每孔接种5×103个MCF-7细胞。在37oC, 5% CO2环境下温育3周后, 细胞混合, 用结晶紫染色,计数阳性菌落(直径>40 μm),使用KS-400图像分析系统测定转化效率。
    (Only for Reference)
动物实验:[1]
- 合并
  • Animal Models: 携带NWT-21细胞的Harlan无胸腺裸鼠,体重为18-25 g
  • Dosages: 20, 30,或50 mg/kg; 10 ml/kg
  • Administration: 每天口服处理2次,每周处理7天
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 88 mg/mL (200.2 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
30% PEG400+0.5% Tween80+5% propylene glycol
20 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 439.55
化学式

C27H29N5O

CAS号 475489-16-8
储存条件 粉状
溶于溶剂
别名 AEW541

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
免费分装抑制剂

IGF-1R Signaling Pathway Map

IGF-1R Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID