SB202190 (FHPI)

目录号:S1077

仅限科研使用

SB202190 (FHPI)是一种有效的p38 MAPK抑制剂,靶向作用于p38α/β,无细胞试验中IC50为50 nM/100 nM,有时用于代替SB 203580研究其在体内对SAPK2a/p38的潜在作用。SB202190 可通过诱导自噬和血红素氧化酶1来抑制内皮细胞的凋亡。SB202190可显著抑制Erastin依赖的铁死亡ferroptosis

SB202190 (FHPI) Chemical Structure

CAS: 152121-30-7

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1091.41 现货
RMB 1189.49 现货
RMB 3017.14 现货
大包装 有超大折扣
大剂量询单有大折扣!

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的SB202190 (FHPI)发表文献141篇:

产品安全说明书

p38 MAPK抑制剂选择性比较

相关p38 MAPK产品

生物活性

产品描述 SB202190 (FHPI)是一种有效的p38 MAPK抑制剂,靶向作用于p38α/β,无细胞试验中IC50为50 nM/100 nM,有时用于代替SB 203580研究其在体内对SAPK2a/p38的潜在作用。SB202190 可通过诱导自噬和血红素氧化酶1来抑制内皮细胞的凋亡。SB202190可显著抑制Erastin依赖的铁死亡ferroptosis
特性 SB 202190 与SB 203580极为相似。
靶点
Ferroptosis [11] p38α [1]
(Cell-free assay)
p38β [1]
(Cell-free assay)
50 nM 100 nM
体外研究

SB 202190显著抑制内源本底和和抗Fas抗体诱导的MAPKAPK 2活性,且抑制效果呈剂量依赖性。SB 202190本身就足以诱导Jurkat细胞和Hela细胞的死亡:通过激活CPP 32样半胱氨酸蛋白酶,可阻断Bcl - 2的表达。p38β可减轻而p38α可加剧由SB 202190诱导的细胞凋亡[2]。在HaCaT细胞中SB 202190强烈抑制由紫外线诱导的cox - 2蛋白表达和 mRNA水平[3]。用SB 202190处理肾小管上皮细胞(正常大鼠k肝脏-52E),可将由白蛋白引发的炎症(单核细胞趋化蛋白-1,MCP - 1)或肿瘤生长因子β-1(TGFβ-1)引发的原纤维化(原骨胶原I a1,procollagen-i alpha1)相关基因降低50%[4]。SB 202190处理A549细胞,可诱导JNK磷酸化(该磷酸化效应呈时间和剂量依赖性)、转录因子ATF - 2磷酸化,并增加AP – 1与DNA的结合[6]。SB 202190处理促进THP-1和MV4-11细胞的生长。SB 202190可提高c-Raf 和ERK(细胞外信号调节激酶)磷酸化,暗示由SB 202190诱导的白血病细胞增殖过程有Ras-Raf-MEK-mitogen激活的MAPK通路的参与[7]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT-116 M{PJdGZ2dmO2aX;uJGF{e2G7 MWGyOUDPxE1? NGDjS5o{OCCvaX6= NIe2dFBFVVORwrC= MmO3ZZR1\W63YYTld{B1cGVibWLORUBidmRicILveIVqdiCneIDy[ZN{cW:wIH;mJIhDTC1{IHnuJJJme3CxboPpeoUhfG9iRFG= NVXiXllpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yNlMzPTFpPkK2NlI{OjVzPD;hQi=>
MDA-MB-231 NXjaR5kxTnWwY4Tpc44hSXO|YYm= Mn7vNkDPxE1? MVSyOEBp NFjqSW5t\XO|ZX7ld{BES0x{IHnu[JVkfGmxbjDifUBVVkcQsR?= NXvmUIprRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xNFA5PDhpPkK2NVAxQDR6PD;hQi=>
rBMSCs MojnSpVv[3Srb36gRZN{[Xl? NXjEPJE{OTBizszN M{nQZVIvPSCq NH3HO5Rl\XC{ZYPz[ZMhfGinIIDoc5NxcG:{eXzheIlwdiCxZjDFVmsh[W6mIICzPC=> NXiyTmdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wOVMzPjZpPkK2NFU{OjZ4PD;hQi=>
MG63 NX7mWWx1TnWwY4Tpc44hSXO|YYm= NInQeIkyOC9{MD:zNEDPxE1? MX:yOEBp M361SpNq\26rZnnjZY51dHliZHXjdoVie2W|IITo[UBt\X[nbDDv[kBxcG:|cHjvdplt[XSnZDDwN|ghcW6mdXPl[EBjgSCFZFPsNkBqdiCjIHPvcoNmdnS{YYTpc44hNWSncHXu[IVvfCCvYX7u[ZI> NF\uNGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm5PFMyOid-MkW5PVg{OTJ:L3G+
MG63 M3rwVGFxd3C2b4Ppd{BCe3OjeR?= MX2xNE8zOC9|MDFOwG0> NHjkSlIzPCCq NH;KNpd{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnZDD0bIUh[XCxcITvd4l{KHKjdHWgc4YhVUd4MzDpcoR2[2WmIHL5JGNlS2x{ NGD6WZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm5PFMyOid-MkW5PVg{OTJ:L3G+
HTSMCs NYPodFBKTnWwY4Tpc44hSXO|YYm= M2jTblAvOS9zL{GwJO69VQ>? M13ib|EhcA>? M1u1PIlvcGmkaYTl[EBEV1KPLUKtbY5lfWOnZDDIU{0yKHC{b4TlbY4hdGW4ZXzzJIFv\CCvUl7BJIV5eHKnc4Ppc44> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl{MUS2OEc,OjV7MkG0OlQ9N2F-
MIA PaCa-2 MnzJSpVv[3Srb36gRZN{[Xl? NUH4SFVoOjEEoN88US=> M4fCd|I1KGh? MWny[YR2[2WmIHzhZ5RifGViYXPjeY12dGG2aX;uJIlvKGOxbXLpcoF1cW:wIIfpeIgh[m:2aDCyMWRIKGGwZDDEMYFtdG:|ZdMg MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
MIA PaCa-2 Mo\mSpVv[3Srb36gRZN{[Xl? MlPHNlDDqM7:TR?= NGewZmkzPCCq M1LnOpJme3WudIOgbY4h[SCvb3Tld5QhcW6qaXLpeIlwdiCxZjDITWYuOc7zIIDyc5RmcW5iYXPjeY12dGG2aX;u M1PhelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
BxPC-3 MVPGeY5kfGmxbjDBd5NigQ>? NWPUenhOOjEEoN88US=> NUHTfVdoOjRiaB?= NHPTbllz\XO3bITzJIlvKGFibX;k[ZN1KGmwaHnibZRqd25ib3[gTGlHNTIQsTDwdo91\WmwIHHjZ5VufWyjdHnvci=> M1LP[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
AsPC-1 NIrXdmZHfW6ldHnvckBCe3OjeR?= M{nTN|IxyqEQvF2= M4\wSlI1KGh? NU\a[IQ{emW|dXz0d{BqdiCjIH3v[IV{fCCrbnjpZol1cW:wIH;mJGhKTi1zzsGgdJJwfGWrbjDhZ4N2dXWuYYTpc44> NHjvepY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
MIA PaCa-2 NIH3U3RHfW6ldHnvckBCe3OjeR?= MnPwNlDDqM7:TR?= NVu4WWNMOjRiaB?= NUXiT3E1\W6qYX7j[ZMh[2ynYY\h[4Uhd2ZiUFHSVEB4cGWwIHPvcYJqdmWmIIfpeIgh\2y3Y3;z[UBidmGub3fz NEXYTJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
MIA PaCa-2 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\wS|MzOMLizszN MlvZNkBp MkfVd4Vve2m2aYrld{Bk\WyuIHzpcoV{KHSxIITy[YF1dWWwdDD3bZRpKDJvRFegZY5lKERvYXzsc5Nm NIC4WlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
BxPC-3 MlzVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfkfJgzOMLizszN NWO1XGVMOiCq M{fW[5NmdnOrdHn6[ZMh[2WubDDsbY5meyC2bzD0doVifG2nboSge4l1cCB{LVTHJIFv\CCGLXHscI9{\Q>? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6OES4PUc,OjV6OEi0PFk9N2F-
AsPC-1 NIXDRY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULOUVJ5OjEEoN88US=> NEnKRoszKGh? NGCxT41{\W6|aYTpfoV{KGOnbHygcIlv\XNidH:geJJm[XSvZX70JJdqfGhiMj3ES{BidmRiRD3hcIxwe2V? M4DlVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi4OFg6Lz5{NUi4PFQ5QTxxYU6=
HEY MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHoU3UzOMLizszN NETkTpIzKGh? M322c5NmdnOrdHn6[ZMh[2WubDDsbY5meyC2bzD0doVifG2nboSge4l1cCB{LVTHJIFv\CCGLXHscI9{\Q>? NGnUcnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
OVCAR-3 NFPPenhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPzR|FuOjEEoN88US=> NFPUW5UzKGh? MVfz[Y5{cXSrenXzJINmdGxibHnu[ZMhfG9idILlZZRu\W62IIfpeIghOi2GRzDhcoQhTC2jbHzvd4U> Mn;NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6OEi0PFkoRjJ3OEi4OFg6RC:jPh?=
SK-OV-3 NX62UHJwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVq5b3l7OjEEoN88US=> M2HnXFIhcA>? M1j0NpNmdnOrdHn6[ZMh[2WubDDsbY5meyC2bzD0doVifG2nboSge4l1cCB{LVTHJIFv\CCGLXHscI9{\Q>? NH3lOpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4PFQ5QSd-MkW4PFg1QDl:L3G+
MH7A  NGHHZ2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;6UY0xOjRiaB?= NXrze5ZnemWrbn\vdoNmeyC2aHWgbY5pcWKrdH;yfUBm\m[nY4TzJI9nKFiDTh?= NIDvXZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi2Nlk3Pid-MkW4OlI6PjZ:L3G+
MH7A  M136XGFxd3C2b4Ppd{BCe3OjeR?= NX\6VHFlOjVizszN NFLvZ3ozPCCq NY\WUFhQemW4ZYLz[ZMh[2WubDDjfYNt\SCjcoLld5QhcW6mdXPl[EBjgSC[QV6gZY5lKGOjdYPl[EBieG:ydH;zbZMhd2ZiY3XscJMhfmmjIHHjeIl3[XSrb36gc4YhUk6N M1TpPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OE[yPVY3Lz5{NUi2Nlk3PjxxYU6=
SCC25 NYnCXmJ1TnWwY4Tpc44hSXO|YYm= NHXDc3EzOCEQvF2= MoLYNlQhcA>? NUm0c20zcW6lcnXhd4V{KGG3dH;wbIFogSCuZY\lcC=> NWXOb5BRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N|Q1ODBpPkK1PFM1PDByPD;hQi=>
HaCaT  MoLUSpVv[3Srb36gRZN{[Xl? Mn;5OUDDvU1? MXuyOEBp M{GyOIlvcGmkaYTzJGlHVi4Qsz3pcoR2[2WmIFPDUFIzKHC{b3T1Z5Rqd25ibHX2[Yx{ NYS0N3hkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4N|Q{PTNpPkK1PFM1OzV|PD;hQi=>
HaCaT  NG\ofmtHfW6ldHnvckBCe3OjeR?= M1XiV|UhyrWP MXuyOEBp MkfibY5pcWKrdIOgTWZPNc7zLXnu[JVk\WRiQ1PMNlIheHKxZIXjeIlwdiCuZY\lcJM> M2r3dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEO0N|U{Lz5{NUizOFM2OzxxYU6=
HPAEpiCs  NIThUHJHfW6ldHnvckBCe3OjeR?= M3TVN|EwOy9zMDFOwG0> MlzUNUBp MnrxdoVlfWOnczDTNXAucW6mdXPl[EBKS0GPLUGgdJJwfGWrbjDhcoQhdVKQQTDlfJBz\XO|aX;uJIFv\CCycn;tc5RmeiCjY4Tpeol1gQ>? M{nUSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{O0PVAxLz5{NUezOFkxODxxYU6=
HPAEpiCs MYDGeY5kfGmxbjDBd5NigQ>? MWOxM|MwOTBizszN MkflNUBp M{j2OolvcGmkaYTzJHMyWC2|dHnteYxifGWmIFHreEBxcG:|cHjvdplt[XSrb36gJOKh MkPDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5M{S5NFAoRjJ3N{O0PVAxRC:jPh?=
HPAEpiCs MkLOSpVv[3Srb36gRZN{[Xl? M1ftW|EwOy9zMDFOwG0> MkXINUBp MXLpcohq[mm2czDTNXAhfGmvZT3k[ZBmdmSnboTsfUB{fGmvdXzheIVlKGNvSoXuJJBpd3OyaH;yfYxifGmxbh?= M4DON|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{O0PVAxLz5{NUezOFkxODxxYU6=
K562 MlW5SpVv[3Srb36gRZN{[Xl? NUnXT4xyOTEEoN88US=> M2Gz[FHDqGh? NIH1UVdFVVOR MUHpcohq[mm2czDxeYlv[WO{aX7lMYlv\HWlZXSgdFM5KE2DUFugdIhwe3Cqb4L5cIF1cW:w NVH0[W5NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFQxPDNpPkK1Olg1ODR|PD;hQi=>
PANC-1 MnPuSpVv[3Srb36gRZN{[Xl? NVvVeHVFOTBizszN M4jx[|EhcA>? NGTWVVVmdmijbnPld{B1cGViYYX0c5Bp[WerYzDl[oZm[3UEoB?= Mlq0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{KyNlIoRjJ3NkOyNlIzRC:jPh?=
BxPC-3 MmDjSpVv[3Srb36gRZN{[Xl? NFjvUIcyOCEQvF2= MYCxJIg> NFrkdoFmdmijbnPld{B1cGViYYX0c5Bp[WerYzDl[oZm[3UEoB?= Mmf1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4M{KyNlIoRjJ3NkOyNlIzRC:jPh?=
K562  NV7XUYtZTnWwY4Tpc44hSXO|YYm= NXvlfG1KOC5{NT2xJO69VQ>? NXPSbW03OjRiaB?= NEe0T2F{fXCycnXzd4V{KHKnc4\ldoF1em:uLXnu[JVk\WRiSELBXEBxcG:|cHjvdplt[XSrb36= MmLxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4MUmzPVIoRjJ3NkG5N|kzRC:jPh?=
THP-1 M2TWeWZ2dmO2aX;uJGF{e2G7 MWK1JOK2VQ>? MnHYNkBp MXvzbYdvcW[rY3HueIx6KGG2dHXueYF1\XNic3XjdoV1cW:wIH;mJGlNNTIQsTDpcoR2[2WmIHL5JFI4V0iFaH;sJJBtfXNiRmPMMVHDqA>? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV7OE[2NUc,OjV3OUi2OlE9N2F-
WB MYHGeY5kfGmxbjDBd5NigQ>? NXG5OYUxOjEkgJpOwG3DqA>? NFjzVYU{OOLCiX3pci=> M2rjdIRm[3KnYYPld{B1cGViTGDTMUBweiCOVFGtbY5lfWOnZDDJUE03KGGwZDDUUmYu|rIEoIDyc4R2[3Srb36= NE\NUIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWzNFY5Oid-MkW1N|A3QDJ:L3G+
RAW 264.7 MYnGeY5kfGmxbjDBd5NigQ>? NGezbIoyOMLizszN NWHS[5VpOzEkgJntbY4> M3ntVIlv\HWlZYOgZ4hiemGldHXybZN1cWNidnHjeY9t[XSrb36gc4YhV0O|wrC= NGr0TGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUS2NVM6QSd-MkW0OlE{QTl:L3G+
RAW 264.7 NUnoW412TnWwY4Tpc44hSXO|YYm= NYrGeFBWOTEEoN88US=> NEDwdXA{OOLCiX3pci=> NFjJ[HVifHSnboXheIV{KHSqZTDl[oZm[3S|IH;mJG9RTyCxbjDvd5Rmd2OuYYP0JJJmfHKjY4Tpc44> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MUO5PUc,OjV2NkGzPVk9N2F-
HaCaT  NUS3Rop7TnWwY4Tpc44hSXO|YYm= MoDPOFDDqM7:TR?= MWizMVI1KGh? MWjEUXNQ NXfxbFZ4emWmdXPld{B1cGViYXPjeY12dGG2aX;uJI9nKFqRLUG= MnTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{W0PFUoRjJ3NEO1OFg2RC:jPh?=
H9c2 M1Pr[mZ2dmO2aX;uJGF{e2G7 NH\YT5Q2OMLizszNxsA> MVyxNkBp M3\6d5Jm\HWlZYOgUGRJKHKnbHXhd4Uh[W6mIF3NVEBtd3O| NHW0dGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUK0OVgyQCd-MkWyOFU5OTh:L3G+
HSCs MoLDRZBweHSxc3nzJGF{e2G7 NF;JT2ozPSEQvF2= NE\H[FUzPMLiaB?= NX\TSHNDe2mpbnnmbYNidnSueTDheJRmdnWjdHXzJHRINWmwZIXj[YQh[WO2aY\heIVlKEiVQ4OgZZBweHSxc3nz NV3R[GhCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlE6PTBpPkK0PVYyQTVyPD;hQi=>
THP-1 NHXqOZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVm3NkBp NGDVe49FVVOR MWfJR|UxRTRwN988US=> NWLicYQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
MDDCs MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2[5NFczKGh? NIP2OVJFVVOR M4freWlEPTB{LkhOwG0> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzNUC4O{c,OjR6MUWwPFc9N2F-
MDDCs MnzUSpVv[3Srb36gRZN{[Xl? NVvrSIxlOC1zNTFOwG0> Mlr2OFghcA>? NU\lWldMTE2VTx?= MUDzeZBxemW|c3XzJGlHVi4QsTDhcoQhUVBvMUCgdJJw\HWldHnvcuKh NFviNWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEixOVA5Pyd-MkS4NVUxQDd:L3G+
MDDCs MX3GeY5kfGmxbjDBd5NigQ>? M3TxVVAuOTVizszN MVS0PEBp NIHONFZFVVOR NWrtXYl4cW6qaXLpeJMhVUmSLUHhMEBOUVBvMXKgZY5lKFKDTmTFV{Bxem:mdXP0bY9v MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDhzNUC4O{c,OjR6MUWwPFc9N2F-
MDDCs Mn7HSpVv[3Srb36gRZN{[Xl? MVmxNEDPxE1? M4fRXlMvPSCq M3HkSmROW09? MVHicI9kc3NiRVLPWkBIWCxiYoX0JI5wfCCYU2[gS{Bu\WSrYYTl[EBmdnS{eTDpcpRwKGi3bXHuJG1FTEO| NUXKVYxQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NVUxQDdpPkK0PFE2ODh5PD;hQi=>
macrophages NWPFVJRDTnWwY4Tpc44hSXO|YYm= M{\4blHjiIoQvF2= NILjNZg1NjYkgJno M3\sUoNwdXCuZYTlcJkhcW6qaXLpeJMhVVRvSVnJMYlv\HWlZXSgZYN1cX[jdHnvckBw\iCQRj5OvmI> NX\3b2lpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4NFg3OzNpPkK0PFA5PjN|PD;hQi=>
PDL  MX7GeY5kfGmxbjDBd5NigQ>? NGnNO4YzOMLizszNxsA> Mmi3N|AhdWmw M13UfmROW09? MmLvd4lodmmoaXPhcpRtgSCrbnjpZol1eyC2aHWgeIVve2muZTDmc5Jk\S2vZXTpZZRm\CCETWCtNkBmgHC{ZYPzbY9v M33sRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NU[xNFgyLz5{NEW2NVA5OTxxYU6=
AGS NFLB[XBHfW6ldHnvckBCe3OjeR?= M1TVNVUh|ryP NYK0XZl2OzBibXnu NGTIWXp{fXCycnXzd4V{yqBzLXnu[JVk\WRiY3HzdIF{\S16IHHu[EBk[XOyYYPlMVMh[WO2aY\heIlwdg>? MlGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3NEe4O|goRjJ2NUS3PFc5RC:jPh?=
H520 MXXGeY5kfGmxbjDBd5NigQ>? NXfoSXR5OTEEoNM1US=> M1vqdFEhcA>? MmfqSG1UVw>? M1;ObIRm[3KnYYPld{B1cGVicHXt[ZRz\XinZD3pcoR2[2WmIF3TTFIhdVKQQTDhcoQheHKxdHXpckBt\X[nbIO= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV|MES3OUc,OjR3M{C0O|U9N2F-
H1703 NVHOVZAxTnWwY4Tpc44hSXO|YYm= MonwNVDDqML3TR?= NV;l[ZpWOSCq NUPzTIFwTE2VTx?= NYXCVGpZ\GWlcnXhd4V{KHSqZTDw[Y1mfHKneHXkMYlv\HWlZXSgUXNJOiCvUl7BJIFv\CCycn;0[YlvKGyndnXsdy=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV|MES3OUc,OjR3M{C0O|U9N2F-
H520 MVjGeY5kfGmxbjDBd5NigQ>? NHzsOHUyOMLiwsXN MlToNVIhcA>? NFzkRZZFVVOR MWPpcohq[mm2czDw[Y1mfHKneHXkMYVtcWOrdHXkJG1UUDJicILveIVqdiC|dHHibYxqfHl? M4H4TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUOwOFc2Lz5{NEWzNFQ4PTxxYU6=
H1703 M3TITGZ2dmO2aX;uJGF{e2G7 MmT4NVDDqML3TR?= M3Xlc|EzKGh? MlizSG1UVw>? NWHDd5dLcW6qaXLpeJMheGWvZYTy[Zhm\C2nbHnjbZRm\CCPU1iyJJBzd3SnaX6gd5Ri[mmuaYT5 MmflQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3M{C0O|UoRjJ2NUOwOFc2RC:jPh?=
H520 MXTGeY5kfGmxbjDBd5NigQ>? NInsTYQyOMLiwsXN NF7Lb2w3KGh? NGrsO3ZFVVOR Mlf2d4lodmmoaXPhcpRtgSCrbnPy[YF{\XNidHjlJIxmfmWuczDv[kB2[mmzdXn0bY4u[2:waoXnZZRm\CCPU1iyJIlvKHCnbXX0doV5\WRvdILlZZRm\MLiY3XscEBtcW6n NXr1flU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1N|A1PzVpPkK0OVMxPDd3PD;hQi=>
H1703 M1;XUmZ2dmO2aX;uJGF{e2G7 MYSxNOKhyrWP MkflOkBp Ml\WSG1UVw>? NHq5[Id{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczD0bIUhdGW4ZXzzJI9nKHWkaYH1bZRqdi2lb37qeYdifGWmIF3TTFIhcW5icHXt[ZRz\XinZD30doVifGWmwrDj[YxtKGyrbnW= M4PkNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUOwOFc2Lz5{NEWzNFQ4PTxxYU6=
MC3T3-E1 Mkm2SpVv[3Srb36gRZN{[Xl? MmjVNE4{NzNxM{Cg{txO NUPnXYRlOSCq NFXEdFFifHSnboXheIV{KFSQRj5OtU1qdmS3Y3XkJG1OWC17IHX4dJJme3Orb36gbY4h[SClb37j[Y51emG2aX;uMYRmeGWwZHXueEBu[W6wZYNCpC=> M37DOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUCyOlk3Lz5{NEWwNlY6PjxxYU6=
MC3T3-E1 NVrpV2MxTnWwY4Tpc44hSXO|YYm= NV\3WnJGOzBizszN MX:xJIg> MYPheJRmdnWjdHXzJHRPTi4QsT3zeIlufWyjdHXkJJA{QCCPQWDLJJBpd3OyaH;yfYxifGmxbh?= NUiweXAxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NFI3QTZpPkK0OVAzPjl4PD;hQi=>
HUVECs  M2T6R2Z2dmO2aX;uJGF{e2G7 MYOxNOKhyrWP NET2dmcyKGh? M4\scIlvcGmkaYTzJHRPTi4QsT3pcoR2[2WmIFPYR2wyKHC{b3T1Z5Rqd25? NFvQVIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES4O|k3PCd-MkS0PFc6PjR:L3G+
AGS  MnTWSpVv[3Srb36gRZN{[Xl? NUfSXGRkOTEEoNM1US=> M1zXOFMxyqCvaX6= MVHpcohq[mm2czDJUE0y|rJvaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QA>? NYHOWllKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|k3QDFpPkK0OFc6PjhzPD;hQi=>
MKN-45 Mn;XSpVv[3Srb36gRZN{[Xl? NIC1W2IyOMLiwsXN NIjvUIw{OMLibXnu M3:wcolvcGmkaYTzJGlNNTIQsj3pcoR2[2WmIHHjeIl3[XSrb36gc4YheDN6 NIDGU4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3PVY5OSd-MkS0O|k3QDF:L3G+
AGS  MkexSpVv[3Srb36gRZN{[Xl? NGHTNnYyOMLiwsXN NVL3VGpoOzEEoH3pci=> NHfnbnRifHSnboXheIV{KEmOLUJOtk1qdmS3Y3XkJGdCKGOnbHygcYloemG2aX;uJIFv\CCrbo\hd4lwdg>? NYHxS3g3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|k3QDFpPkK0OFc6PjhzPD;hQi=>
MKN-45 MXPGeY5kfGmxbjDBd5NigQ>? MnHsNVDDqML3TR?= MUizNOKhdWmw NYDlempD[XS2ZX71ZZRmeyCLTD2x{tIucW6mdXPl[EBISSClZXzsJI1q\3KjdHnvckBidmRiaX72ZZNqd25? MmT1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{m2PFEoRjJ2NEe5OlgyRC:jPh?=
AGS  NITnVodHfW6ldHnvckBCe3OjeR?= MXixNOKhyrWP MYWzNOKhdWmw NHi4eXN{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczDJcE0y|rJvaX7keYNm\CCPTWCyJIFv\CCPTWC5JI1TVkFiZYjwdoV{e2mxbh?= M2C5T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEe5OlgyLz5{NES3PVY5OTxxYU6=
MKN-45 MVnGeY5kfGmxbjDBd5NigQ>? M2HCS|ExyqEEtV2= NWPnZ2tIOzEEoH3pci=> NUXCR2hqe2mpbnnmbYNidnSueTDk[YNz\WG|ZYOgTYwuOc7{LXnu[JVk\WRiTV3QNkBidmRiTV3QPUBuWk6DIHX4dJJme3Orb36= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5OU[4NUc,OjR2N{m2PFE9N2F-
DCs NI\lfJNHfW6ldHnvckBCe3OjeR?= M1nzZlIxyqEQvF2= NEPuUoYyyqCq NXG3eoJO\GWlcnXhd4V{KEmOLUGyJJBzd2S3Y4Tpc44> NGC4[Ys9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESzOFY{Pid-MkS0N|Q3OzZ:L3G+
HUVEC  NVj3WnFDTnWwY4Tpc44hSXO|YYm= M1:5dVIxKM7:bdMg NYrU[5JtPSCq NGCxTIVFVVOR NXnq[pZ5emWmdXPld{BkgXSxa3nu[UBmgHC{ZYPzbY9vKGyndnXsd{BqdiCjIHPvcoNmdnS{YYTpc44u\GWyZX7k[Y51KG2jbn7ldi=> MlezQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOEmwOlIoRjJ2MUi5NFYzRC:jPh?=
A 549 MoLhSpVv[3Srb36gRZN{[Xl? MoDyOVAh|ryP MVSxJIg> NUfCNXJC\GWlcnXhd4V{KHSqZTDs[ZZmdCCxZjDJUE05yqB? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF5OU[4PEc,OjRzN{m2PFg9N2F-
H520  NYfHN2ZFTnWwY4Tpc44hSXO|YYm= M1P3fVUwOTBizszN MlH3NUBp MXnEUXNQ NHrMdGZl\WO{ZXHz[ZMhVVOKMjDwdo91\WmwIHHzJJdmdGxiYYOgcXJPSSCuZY\lcJMhcW5iZ3XmbZRqdmmkLXX4dI9{\WRiY3XscC=> MlPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{i5NFMoRjJ2MUO4PVA{RC:jPh?=
H1703  NXizOWo6TnWwY4Tpc44hSXO|YYm= MkDnOU8yOCEQvF2= MUexJIg> M4[xfmROW09? MWnk[YNz\WG|ZYOgUXNJOiCycn;0[YlvKGG|IIflcIwh[XNibWLORUBt\X[nbIOgbY4h\2WoaYTpcoljNWW6cH;z[YQh[2WubB?= NXy2SmdHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxN|g6ODNpPkK0NVM5QTB|PD;hQi=>
H520  NEHXb5BHfW6ldHnvckBCe3OjeR?= NFHIPYYyOMLiwsXN MmHXNVIhcA>? NXrxZ5pqTE2VTx?= MV3k[YNz\WG|ZYOgUXNJOiCvUl7BJIFv\CCycn;0[YlvKHO2YXLpcIl1gSCrbjDn[YZqfGmwaXKteJJm[XSnZDDOV2NNSyClZXzsdy=> MnfsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzM{i5NFMoRjJ2MUO4PVA{RC:jPh?=
H1703  M1vWfmZ2dmO2aX;uJGF{e2G7 MYixNOKhyrWP NV\sVmZUOTJiaB?= NFTGXmRFVVOR MkPa[IVkemWjc3XzJG1UUDJibWLORUBidmRicILveIVqdiC|dHHibYxqfHliaX6g[4VncXSrbnniMZRz\WG2ZXSgUnNEVENiY3XscJM> M1XmO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUO4PVA{Lz5{NEGzPFkxOzxxYU6=
MCF-7  NImz[oVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fkZVExyqEQvF2= MVSyOOKhcA>? NHvM[FdqdmirYnn0d{B1cGViQ2KxNFgucW6mdXPl[EBk\WyuIHTlZZRp MojuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMki4OVMoRjJ2MUK4PFU{RC:jPh?=
HPAEpiCs MnPHSpVv[3Srb36gRZN{[Xl? M1\XSlAvOS9zL{GwJO69VQ>? MljvNUBp NG\GRpVqdmirYnn0d{BVVkZvzsGtbY5lfWOnZDDjVGxCOsLicILveIVqdiCjbnSgcXJPSSCneIDy[ZN{cW:w M4XneFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2ME[5NVU5Lz5{NEC2PVE2QDxxYU6=
podocytes M3nBd2Z2dmO2aX;uJGF{e2G7 NXvnOJBXOTEEoN88US=> MVyxJIg> NF[5W2hqdmirYnn0d{BVT0cQskGtbY5lfWOnZDDhZ5RqfmG2aX;uJI9nKHB|OF3BVGsh[W6mIFXyb|EwOsLi MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB|NkKxNkc,OjRyM{[yNVI9N2F-
MCF-7 M1TmRmZ2dmO2aX;uJGF{e2G7 MUexNOKh|ryP MX2xJIg> MV7EUXNQ NXvEUXBIemWmdXPld{B1cGViV1GtbY5lfWOnZDDwbI9{eGixconsZZRm\CCyM{igUWFRUw>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzOUC5NEc,OjRyMUmwPVA9N2F-
MCF-7 NX\L[XFtTnWwY4Tpc44hSXO|YYm= MlS5NVDDqM7:TR?= M3zuRlI1KGh? NFTEUYRFVVOR NIm0S|dqdmO{ZXHz[ZMhfGinIGfBMYlv\HWlZXSgZZBweHSxc3nz NUe3PHZsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVkxQTBpPkK0NFE6ODlyPD;hQi=>
HAPI M1nXSWZ2dmO2aX;uJGF{e2G7 NXT2VZhQOTBxMkCvOFDDqM7:TR?= MXWxJIg> NWH5N|VZcW6qaXLpeJMhXEOGRD3pcoR2[2WmIICzPE9LVktiTVHQT{BxcG:|cHjvdplt[XSrb36= MlTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkmxNlAoRjJ|OU[5NVIxRC:jPh?=
HAPI NGfqPYpHfW6ldHnvckBCe3OjeR?= Mnr2NlDDqM7:TdMg M{nOSFEhcA>? NUfmb4oyTE2VTx?= M2HnR4F1fGWwdXH0[ZMhXEOGRD3pcoR2[2WmIHHjeIl3[XSrb36gc4YhcU6RUzDhcoQheHKxZIXjeIlwdg>? MkHQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NkmxNlAoRjJ|OU[5NVIxRC:jPh?=
HepG2 NEnzcllHfW6ldHnvckBCe3OjeR?= NF;DOJo{PTBibl2= NFryWHozPCCq NFn3Z5BqdmirYnn0d{B1cGViZHXneYVtcW5vaX7keYNm\CCjY4TpeoF1cW:wIH;mJJA{QE2DUFu= NIO0dmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mzN|E6QCd-MkO5N|MyQTh:L3G+
AGS  MnvQSpVv[3Srb36gRZN{[Xl? M1r4U|ExyqEQvF2= NED1foY{OCCvaX6= MnnNbY5pcWKrdIOgZ4F{eGG|ZT2zJIFkfGm4YYTpc44h[W6mIHnubIljcXSrb36gc4YhTVKN MkS5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NUC5PVQoRjJ|OEWwPVk1RC:jPh?=
HepG2 M4fnVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYmwMVUxKM7:TR?= MXe0PEBp M1XIWYlvcGmkaYTzJJRp\SCycn;sbYZmemG2aX;uJIlvKGFiZH;z[UBl\XCnbnTlcpQhdWGwbnXy MnPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MEe1NFgoRjJ|OEC3OVA5RC:jPh?=
BEL7404 MlTxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWPqUoFNOC13MDFOwG0> MXW0PEBp MlX3bY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gbY4h[SCmb4PlJIRmeGWwZHXueEBu[W6wZYK= NHruXJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwO|UxQCd-MkO4NFc2ODh:L3G+
HL7702 MnnsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTmXmJGOC13MDFOwG0> MXW0PEBp MX\pcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDpckBiKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= NF;RWmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwO|UxQCd-MkO4NFc2ODh:L3G+
HepG2 MmqxSpVv[3Srb36gRZN{[Xl? NGn6XIQxNTVyIN88US=> MmPJNlQhcA>? NXmy[pVqcW6qaXLpeJMhfGinIIDoc5NxcG:{eXzheIlwdiCxZjDwN|gh\G:5boP0doVidSCycn;0[YlveyCPQWDLRXBMOixiQWTGNkwhVVONMTDhcoQhUFOSMkegbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= M1TmbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEC3OVA5Lz5{M{iwO|UxQDxxYU6=
BEAS-2B NHLsZWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEfaR|QyOCEQvF2= MnXFN|AhdWmw M2n2cZJmfmW{c3XzJJRp\SCmZXPy[YF{\SCxZjDj[YxtKH[rYXLpcIl1gSCrbnT1Z4VlKGK7IFjDTS=> NGPIW|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e4OFA{PCd-MkO3PFQxOzR:L3G+
BEAS-2B Ml\xR5l1d3SxeHn0fUBCe3OjeR?= M{X3ZVExKM7:TR?= MXizNEBucW5? NFLlTY9qdmirYnn0[YQhfGinIHnuZ5Jm[XOnIHnuJGxFUCCjbnSgTWwuQCCneIDy[ZN{cW:w MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6NECzOEc,OjN5OESwN|Q9N2F-
BEAS-2B MXLGeY5kfGmxbjDBd5NigQ>? NUfY[29yOTBizszN MkTVN|AhdWmw M1jDcIRm[3KnYYPld{B1cGVibHX2[Yx{KG:oIHPhd5Bie2VvMzygRoFlKGGwZDDmZZM> MkLtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OESwN|QoRjJ|N{i0NFM1RC:jPh?=
H9c2  MmrzSpVv[3Srb36gRZN{[Xl? NXzv[ml[OC5yMT:wMlEwOSEQvF2= MluzNUBp MnjaZZR1\W63YYTld{BVVkZvzsGtbY5lfWOnZDDNUXAuQSCneIDy[ZN{cW:wLDDtVm5CKGyndnXsd{wh[W6mIIDyc41wfGW{IHHjeIl3cXS7 NGP3XmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e3OFI2Oid-MkO3O|QzPTJ:L3G+
H9c2  MnjySpVv[3Srb36gRZN{[Xl? NWHoc|d7OSEQvF2= MVexJIg> NYrNc49oemWmdXPld{BVVkZvzsGg[Ilz\WO2bImgd5RqdXWuYYTl[EBxOzhiTVHQT{BxcG:|cHjvdplt[XSrb36= Mln3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5N{SyOVIoRjJ|N{e0NlUzRC:jPh?=
U937 Mk\PSpVv[3Srb36gRZN{[Xl? NXLyT2tsOTEEoN88UeKh MWKxJIg> MULhZpJw\2G2ZYOgeIhmKGOjZn\lbY5mKGWoZnXjeEBwdiCPS2CtNUBidmRiUGCyRYPPuSCvUl7BJJRz[W6|Y4LpdJRqd26jbDDs[ZZmdHN? NGrPeos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO|M5Pyd-MkO3NFc{QDd:L3G+
U937 MVHGeY5kfGmxbjDBd5NigQ>? M2HhU|ExyqEQvF5CpC=> M1Hm[lEhcA>? MY\hZpJw\2G2ZYOgZ4Fn\mWrbnWtbY5lfWOnZDDNT3AuOSCmb4fuMZJm\3WuYYTpc44h[W6mIGDQNmFk|rFidYCtdoVofWyjdHnvci=> NHu5OZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO|M5Pyd-MkO3NFc{QDd:L3G+
U937 MVLGeY5kfGmxbjDBd5NigQ>? MkTJNVDDqM7:TdMg NFrsSG8yKGh? NGm5T4l{fXCycnXzd4V{KGNvSoXuJIFv\CCFUlXCJJBpd3OyaH;yfYxifGmxbjDpckBk[W[oZXnu[U11emWjdHXkJINmdGy|wrC= NV3KZXpuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFc{QDdpPkKzO|A4Ozh5PD;hQi=>
A549 MlvtSpVv[3Srb36gRZN{[Xl? MmHENE4{NzNxM{Cg{txO MX6xJIg> M4\qN5Nq\26rZnnjZY51dHliYYT0[Y52[XSnczDBWHDPu1NvbXXkbYF1\WRiQ1;YMVIheHKxdHXpckBidmRibWLORUBmgHC{ZYPzbY9vKGGwZDDwdo9ud3SncjDhZ5Rqfmm2edMg MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6ME[3OEc,OjN4OEC2O|Q9N2F-
A549 M3nSUWZ2dmO2aX;uJGF{e2G7 MkHSNVDDqM7:TdMg NEj6VFkxNTNyIH3pci=> NE\ldJNqdmirYnn0d{BCXFEQs2OgbY5lfWOnZDDwOFIweDR2IF3BVGsh[W6mIICzPEBOSVCNIIDoc5NxcG:{eXzheIlwdg>? NXrnbY41RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFA3PzRpPkKzOlgxPjd2PD;hQi=>
A549 M37Kb2Z2dmO2aX;uJGF{e2G7 MYqxNOKh|ryPwrC= M33kdlEhcA>? NWLrU21jcW6qaXLpeJMhSVSSzsPTJIlv\HWlZXSgUmYu|rqEIIC2OUB{fWK3bnn0JJBpd3OyaH;yfYxifGmxbjDhcoQhVkZvzsrCJJBzd22xdHXyJIFkfGm4aYT5 NIXJNGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NFY4PCd-MkO2PFA3PzR:L3G+
A549 Mnm2SpVv[3Srb36gRZN{[Xl? MXSxNOKh|ryPwrC= M1jwUVEhcA>? NYTkVY1qemWmdXPld{BCXFEQs2Otd5RqdXWuYYTl[EBkWEyDMjDwbI9{eGixconsZZRqd28EoB?= NHy4UVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NFY4PCd-MkO2PFA3PzR:L3G+
A549 NFjyUWJHfW6ldHnvckBCe3OjeR?= NUWwUGVuOTEEoN88UeKh NV7GNmR3OSCq NWnPZ4dLemWmdXPld{BCXFEQs2Ot[Y5p[W6lZXSg[Y57gW2jdHnjJIFkfGm4aYT5JI9nKGOSTFGyxsA> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6ME[3OEc,OjN4OEC2O|Q9N2F-
PC12 M2LmUGZ2dmO2aX;uJGF{e2G7 Mn;YNVAwOjBxNECg{txO MmjONeKhcA>? NET1S3ZqdmirYnn0d{BLVktiYX7kJJA{QA>? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV6NEO1O{c,OjN3OESzOVc9N2F-
HK-2 MlrCRZBweHSxc3nzJGF{e2G7 NEPJN|MzOMLizszN M4LybFI1KGh? M4jTTYlvcGmkaYTzJGVTUyCjbnSgdFM5VUGSSx?= MmnZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3NEOxOVEoRjJ|NUSzNVUyRC:jPh?=
H9c2 NH;UT5lHfW6ldHnvckBCe3OjeR?= MYGxxsDPxE1? NHzsem0yKGh? NIXmfmhz\WS3Y3XzJHRPTi4QsT3pcoR2[2WmIF3NVE06KG2UTlGgcIV3\Wy|IHHu[EBxem:vb4TldkBi[3Srdnn0fS=> NYfwXJVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzOVM3QTlpPkKzN|U{Pjl7PD;hQi=>
H9c2 NGnzfnpHfW6ldHnvckBCe3OjeR?= M{P4blHDqM7:TR?= M{G4OVEhcA>? NWDvSFJKemWmdXPld{BVVkZvzsGt[Y5p[W6lZXSgRXAuOSCycn;tc5RmeiCjY4Tpeol1gQ>? M2SzbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{WzOlk6Lz5{M{O1N|Y6QTxxYU6=
H1650  M2LvW2Z2dmO2aX;uJGF{e2G7 NHPoUHgyOMLizszNxsA> NXjq[HRFOSCq M{\MdmROW09? MVrk[YNz\WG|ZYOgZo91cCCycn;0[YlvKGGwZDDtVm5CKGyndnXsd{Bw\iCHUlPDNUBqdiCyYXPsbZRigGWuLXX4dI9{\WUEoHPlcIx{ NUC1enZbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyNlg3QTZpPkKzNlI5Pjl4PD;hQi=>
H1703 NFHFSWlHfW6ldHnvckBCe3OjeR?= MYixNOKh|ryPwrC= NIf4RogyKGh? MlHKSG1UVw>? MYjk[YNz\WG|ZYOgZo91cCCycn;0[YlvKGGwZDDtVm5CKGyndnXsd{Bw\iCHUlPDNUBqdiCyYXPsbZRigGWuLXX4dI9{\WUEoHPlcIx{ NVnveWpYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyNlg3QTZpPkKzNlI5Pjl4PD;hQi=>
H1650  MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXQUFFpOTEEoN88UeKh M{n6fFEhcA>? MkT3SG1UVw>? Mlv1[Y5p[W6lZYOgdIFkdGm2YYjlcE1qdmS3Y3XkJIN6fG:2b4jpZ4l1gQ>? NHXxV5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KyPFY6Pid-MkOyNlg3QTZ:L3G+
H1703 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV6xNOKh|ryPwrC= M3zndFEhcA>? MX;EUXNQ NX7vPIh5\W6qYX7j[ZMheGGlbHn0ZZhmdC2rbnT1Z4VlKGO7dH;0c5hq[2m2eR?= NGmxVYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{KyPFY6Pid-MkOyNlg3QTZ:L3G+
medulloblastoma cells MVrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnTLRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCvZXT1cIxw[myjc4TvcYEh[2WubIOgbIFz[m:{aX7nJIhmfGW{b4r5[492eyCydHPoNUBo\W6nIHL5JG1VXCCjc4PhfUwhTUN3MDC9JFMvODB4IN88UU4> M165S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEG3OlMyLz5zN{SxO|Y{OTxxYU6=
neural precursor cells NEn4doNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKG6ndYLhcEBxemWldYLzc5Ih[2WubIOgZpkhVVSWIHHzd4F6NCCHQ{WwJF0hQC5yNkOg{txONg>? NEDY[VQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SxO|Y{OSd-MUe0NVc3OzF:L3G+
RAW264.7 MmLHRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NGLCWIMyOCCvaX7z NHLiS|VCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCrbnP1ZoF1\WRiZn;yJFExKG2rboOgdJJqd3JidH:gUHBUNWOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAyQCCqcoOgZpkhT3KrZYPzJI1mfGixZDygTWM2OCB;IEG2JO69VS5? NXy2ZWplRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4N|E4QThpPkKyPFMyPzl6PD;hQi=>
BL21(DE3) M1zZOmZ2dmO2aX;uJIF{e2G7 M1L3fFYxKG2rboO= Ml7KTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCQLYTldo1qdmGuIFjpd|YufGGpZ3XkJGJTTDRiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgRmwzOSiGRUOpJINmdGy|IIXzbY5oKGKrb4Tpcplt[XSnZDDobZN1d26nIFi0JJBmeHSrZHWgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkhSWyyaHHTZ5Jm\W5iYYPzZZktKEmFNUCgQUA{NjRizszNMi=> NGrXdnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEG5OVczOyd-MkixPVU4OjN:L3G+
RAW264.7 NUHyXmY5TnWwY4Tpc44h[XO|YYm= M4jOcXBzd3SnY4Tpc44h[WejaX7zeEBD[WOrbHz1d{BidnSqcnHjbZMheHKxdHXjeIl3\SCjboTp[4VvKGGwZDDs[ZRp[WxidH;4bY4u\GmyaITo[ZJq[SC2b4jpckBkcGmvZYLpZ{Bxem:2ZXnuJI1m\GmjdHXkJIN6fG:2b4jpZ4l1gSCrbjDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fS=> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR6NUWwOEc,OTd2OEW1NFQ9N2F-
A673 M4q2RZFJXFNiYYPzZZk> NXfjdWlPeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFG2O|Mh[2WubIO= M3LWPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
DAOY MYfxTHRUKGG|c3H5 M3z0[JFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCGQV;ZJINmdGy| NYHyNXluRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD MkntdWhVWyCjc4PhfS=> NV3zOHhDeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLEJINmdGy| M2XTblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 NWew[WhWeUiWUzDhd5NigQ>? NX\wNoN5eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsdy=> NYr4ZWRRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-12 NHr2XY1yUFSVIHHzd4F6 M16zR5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTF{IHPlcIx{ MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
LAN-5 NV3yV3loeUiWUzDhd5NigQ>? M{TBbJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGxCVi13IHPlcIx{ MlryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh41 MVHxTHRUKGG|c3H5 MXrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? MmK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 NV\ZR5RneUiWUzDhd5NigQ>? Mn;1dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIuTUKlMTDj[Yxtew>? NEOybmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-SH MY\xTHRUKGG|c3H5 M3ToVZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vU1igZ4VtdHN? NYHXboJNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
A673 MWjxTHRUKGG|c3H5 Mn\hdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgRVY4OyClZXzsd{k> M3i3ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 NHnCdVJyUFSVIHHzd4F6 NFnycYNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1|NzDj[Yxtew>? MnHhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R) MmHodWhVWyCjc4PhfS=> NG\XOnhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBOTyB4MzCoOk1VTyCUKTDj[Yxtew>? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh30 M2fNdZFJXFNiYYPzZZk> NXzuXYZzeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmh|MDDj[Yxtew>? NXi0N|lERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
OHS-50 MlT3dWhVWyCjc4PhfS=> MYnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDPTHMuPTBiY3XscJM> M3f6c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh41 NWG0[XE4eUiWUzDhd5NigQ>? NF3VOppyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBTcDRzIHPlcIx{ M{LyfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC NXe2XmwyeUiWUzDhd5NigQ>? MlKwdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= MlGzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 M13aeZFJXFNiYYPzZZk> MoDPdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIyPjR|IHPlcIx{ NXjCXlFFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC MnHpdWhVWyCjc4PhfS=> M{XWcZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNMNU5vTVOgZ4VtdHN? NHjRZ3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SJ-GBM2 MkP5dWhVWyCjc4PhfS=> NVLhNWtteUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= NU\OTHVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 M3zJcZFJXFNiYYPzZZk> NW[5e2poeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN|MjDj[Yxtew>? M4rNbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 MYPxTHRUKGG|c3H5 NUPqfI5neUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Assay
Methods Test Index PMID
Western blot mTOR / p-S6K / S6K / p-S6 / p-AKT / p-MK2 pJNK1 / pJNK2 / JNK1 / JNK2 26844273 18222647
Immunofluorescence p-p38 / MMP9 24479681
Growth inhibition assay Cell viability 26844273
体内研究

SB 202190通过抑制p38减弱了由人脊髓灰质炎IgG(PV-IgG)在被动转移小鼠模型中诱发的水泡形成[5]。在脓毒症内毒素模型中,与对照组相比,经SB 202190处理可显著提高存活率[8]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

  • 体外酶活检测:

    p38α 和 p38β 溶解在25mM的Tris - HCl缓冲液中,PH 7.5,含0.1mM EGTA,髓鞘碱性蛋白(0.33毫克/毫升)作为底物。人工操作条件用50μL [γ-33P]ATP于30 °C孵育10分钟;Biomek 2000自动化工作站用25μL [γ-33P]ATP在96孔板室温孵育40分钟。ATP和乙酸镁浓度分别为0.1mM和10mM。所有检测都用MgATP起始。人工检测将孵育液滴到磷酸纤维素纸上,浸泡在50mM磷酸中。用5μL磷酸终止反应,后将孵育液点滴在P30过滤垫上。滤膜在50mM磷酸中漂洗四次以去除ATP,在用丙酮(人工孵育)或甲醇(工作站孵育)处理后,干燥并用放射性计量。

细胞实验:

[2]

  • Cell lines: Jurkat, 和 HeLa细胞
  • Concentrations: 溶解于DMSO, 终浓度~50 μM
  • Incubation Time: 24 小时
  • Method:

    细胞血清饥饿后,用不同浓度SB 202190处理24小时。用台盼蓝或PI染色后经流式细胞仪分析,以检测细胞活力。凋亡细胞核用H33258染色。

动物实验:

[5]

  • Animal Models: C57BL/6J 小鼠皮内注射无菌的对照IgG或人脊髓灰质炎IgG做对比。
  • Dosages: 12.5 μg
  • Administration: 皮内注射

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%H2O

8 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 331.34
化学式

C20H14N3OF

CAS号 152121-30-7
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

* 必填项

请输入您的姓名
请输入您的邮箱地址 请输入一个有效的邮箱地址
请写点东西给我们