A-769662

For research use only. Not for use in humans.

目录号:S2697

A-769662 Chemical Structure

CAS No. 844499-71-4

A-769662是一种有效的,可逆的AMPK激活剂,在无细胞试验中EC50为0.8 μM,对GPPase/FBPase活性几乎没有作用。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1719.08 现货
RMB 1200.38 现货
RMB 2235.32 现货
RMB 3860.59 现货
RMB 10401.3 现货
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客户使用Selleck生产的A-769662发表文献57篇:

产品安全说明书

AMPK抑制剂选择性比较

生物活性

产品描述 A-769662是一种有效的,可逆的AMPK激活剂,在无细胞试验中EC50为0.8 μM,对GPPase/FBPase活性几乎没有作用。
靶点
AMPK [1]
(Cell-free assay)
Fatty acid synthesis [1]
(in primary rat hepatocytes)
0.8 μM(EC50) 3.2 μM
体外研究

A-769662部分刺激纯化的大鼠肝脏AMPK,EC50 为0.8 μM。A-769662以剂量响应的方式激活从多种组织和物种纯化得到的AMPK,伴随不同的EC50s。A-769662的EC50s,使用从大鼠心脏,大鼠肌肉,或人胚肾细胞(HEKs)部分纯化的AMPK测定,结果分别为2.2 mM,1.9 mM,或1.1 mM。原代大鼠肝细胞用A-769662处理4小时剂量依赖性增加ACC磷酸化,其与脂肪酸合成的抑制相关,IC50为3.2 μM。A-769662也会抑制小鼠肝细胞中脂肪酸合成,IC50为3.6 μM。[1] A-769662通过变构调节和抑制AMPK在Thr-172上的去磷酸化作用激活AMPK,类似于AMP的作用。[2] A-769662通过与AMPK无关的作用机制抑制蛋白酶体功能。 A-769662影响纯化26S蛋白酶体的体外活性,但是不影响纯化20S蛋白酶体的体外活性。A-769662对MEF细胞具有毒性作用。[3]一项最近的研究表明,A-769662抑制细胞增殖和DNA合成。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse hepatocytes NFXXT|BHfW6ldHnvckBie3OjeR?= MoTjNUBuVQ>? MX\EUXNQ NEHBOm5qdmirYnn0d{Bn[XS2eTDhZ4llKHO7boTo[ZNqeyC5aYToJGlEPTBib3[gN{43KM7:TR?= NXGzVXFuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[3OVM2PzZpPkG2O|U{PTd4PD;hQi=>
rat hepatocytes M2\ve2Z2dmO2aX;uJIF{e2G7 Mm\NNUBuVQ>? MmjlSG1UVw>? M1\BWIlvcGmkaYTzJIZifHS7IHHjbYQhe3mwdHjld4l{KHerdHigTWM2OCCxZjCzMlYh|ryP NHz1dZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNke1N|U4Pid-MU[3OVM2PzZ:L3G+
HEK293 M2jlXmtqdmG|ZTDhd5NigQ>? NYC1O5hnOjByIN88US=> MYLEUXNQ NX:2[HJG[WO2aY\heIV{KGWwZH;n[Y5wfXNiQV3QTy=> M1H3T|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5N{K4NlQyLz5zN{eyPFI1OTxxYU6=
CCL13 NFfnbFNMcW6jc3WgZZN{[Xl? M4PMfVIxOCEQvF2= MUPEUXNQ NYq3Opg1[WO2aY\heIV{KGWwZH;n[Y5wfXNiQV3QTy=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzd{OEK0NUc,OTd5MkiyOFE9N2F-
MEFs MoHySpVv[3Srb36gZZN{[Xl? M1TFXlMxOCEQvF2= NX:zb2ZuTE2VTx?= NEjNTlVqdmirYnn0d{Bxem:2ZXHzc41idCCodX7jeIlwdiCkeTDhckBCVVCNLXnu[IVx\W6mZX70JI1m[2ijbnnzcS=> NF3UOlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW5N|U5PCd-MUi1PVM2QDR:L3G+
epididymal clear cells NGLrd3hHfW6ldHnvckBie3OjeR?= M1O0cVIxOCEQvF2= MUTEUXNQ NInlS5dqdmirYnn0d{B1cGVicFitcYVlcWG2ZXSgWk1CXFCjc3WgZYNkfW23bHH0bY9vKGG2IITo[UBieGmlYXygcYVu[nKjbnW= M4TCRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MkGxPVE5Lz5zOUKxNVkyQDxxYU6=
3T3-L1 MlfUSpVv[3Srb36gZZN{[Xl? MmHJNU4zKG2P MXHEUXNQ MYXpcohq[mm2czCzWFMuVDFiQXTpdI9o\W6nc3nz MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6M{OwOEc,OTl2OEOzNFQ9N2F-
3T3-L1 NYjaNWE4TnWwY4Tpc44h[XO|YYm= MlHtNU4zKG2P MXTEUXNQ MlLUbY5pcWKrdIOgeIhmKEW6cILld5Nqd25ib3[gRYRqeG:pZX7ld4l{WmWuYYTl[EBVemGwc3PybZB1cW:wIF\hZ5RwenNiYX7kJG1iemuncoO= NVW0XWZQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0PFM{ODRpPkG5OFg{OzB2PD;hQi=>
3T3-L1 M{Xvb2Z2dmO2aX;uJIF{e2G7 NHTWTJMyNjJibV2= MmfPSG1UVw>? Ml7TbY5pcWKrdIOgUYl1d3SrYzDDcI9v[WxiRYjwZY5{cW:w MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6M{OwOEc,OTl2OEOzNFQ9N2F-
3T3-L1 M{iwNoN6fG:2b4jpZ4l1gSCjc4PhfS=> NXnjc|VOOS5{IH3N MnnESG1UVw>? MlXH[IVkemWjc3XzJGNmdGxiVnnhZoltcXS7 NI\qOVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS4N|MxPCd-MUm0PFM{ODR:L3G+
3T3-L1 NXLoOVJWU2mwYYPlJIF{e2G7 M4HzU|EvOiCvTR?= NEfCeXlFVVOR MWnhZ5RqfmG2ZYOgRW1RUw>? NH\ldpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS4N|MxPCd-MUm0PFM{ODR:L3G+
L6 skeletal muscle cells NUW1T2pXTnWwY4Tpc44h[XO|YYm= MUKyOVAh|ryP MWnEUXNQ MUjhZ5RqfmG2ZYOgRW1RUyC|aXfuZYxqdmdicHH0bJdigXN? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh{OEizOkc,OTl6Mki4N|Y9N2F-
L6 skeletal muscle cells M2\STGZ2dmO2aX;uJIF{e2G7 NH:4NWQzPTBizszN MlW0SG1UVw>? NVTXfJMxcW6qaXLpeJMhfGinIF7hL{1MMy2DVGDhd4UhfHKjboPwc5J1KGGldHn2bZR6KGGwZDDj[YxtKHO3cn\hZ4Uh[WK3bnThcoNm NV7hRWFoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4Nlg5OzZpPkG5PFI5QDN4PD;hQi=>
MDA-MB231 MW\BdI9xfG:|aYOgZZN{[Xl? M1mwXFQxOCEQvF2= NILJ[XRFVVOR M1vieZNmdnOrdHn6[ZMhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmW|IITvJHRTSUmOLXnu[JVk\WRiYYDvdJRwe2m| NITKeoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUi5OlQ3QSd-MUm4PVY1Pjl:L3G+
BT474 MUTBdI9xfG:|aYOgZZN{[Xl? MXK0NFAh|ryP Ml60SG1UVw>? M2PHVJNmdnOrdHn6[ZMhcHWvYX6gZpJm[XO2IHPhcoNmeiClZXzsJIxqdmW|IITvJHRTSUmOLXnu[JVk\WRiYYDvdJRwe2m| NFvENYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUi5OlQ3QSd-MUm4PVY1Pjl:L3G+
MCF7 MYXBdI9xfG:|aYOgZZN{[Xl? M1\heVQxOCEQvF2= MkLsSG1UVw>? NXjtV4pVe2Wwc3n0bZpmeyCqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hXFKDSVytbY5lfWOnZDDhdI9xfG:|aYO= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh7NkS2PUc,OTl6OU[0Olk9N2F-
Mesenchymal stem cells M{\XN2tqdmG|ZTDhd5NigQ>? MWKxNEDDvU1? NXTYWIFUTE2VTx?= NEC1bpVqdmS3Y3XzJIEhem:kdYP0JIFv\CC|dYP0ZYlv\WRiQV3QT{Bi[3SrdnH0bY9v Mn3MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMES4O|koRjJ2MUC0PFc6RC:jPh?=
Mesenchymal stem cells M3vmboN6fG:2b4jpZ4l1gSCjc4PhfS=> MUixNFAhyrWP NUi3W5NzTE2VTx?= MlfS[IVkemWjc3XzJJRp\SCPU1OgdJJwdGmoZYLheIlwdg>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyNEi3PUc,OjRzMES4O|k9N2F-
MG-63 MYHjfZRwfG:6aXPpeJkh[XO|YYm= NEPiXmEyOCEEtV2= MX3EUXNQ M1;0eolvcGmkaYTzJGgzVzJvSX7keYNm\CCRc4Tlc4Jt[XO2IFPlcIwhTGWjdHi= MlziQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkCzOlIoRjJ2OU[wN|YzRC:jPh?=
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MG-63 NHrieVBCeG:ydH;zbZMh[XO|YYm= MmraNVAhyrWP MkLXSG1UVw>? M3q5XJN2eHC{ZYPz[ZMhUDKRMj3JcoR2[2WmIF;zeIVw[myjc4SgR4VtdCCDcH;weI9{cXN? MnLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkCzOlIoRjJ2OU[wN|YzRC:jPh?=
MC3T3-E1 M1vIfGFxd3C2b4Ppd{Bie3OjeR?= MX:xNEDDvU1? MkXjSG1UVw>? NV\3d|lze3WycILld5NmeyCKMl:yMWlv\HWlZXSgU5N1\W:kbHHzeEBE\WyuIFHwc5B1d3Orcx?= M2TpNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wN|YzLz5{NEm2NFM3OjxxYU6=
MG-63 NYC0bXRKTnWwY4Tpc44h[XO|YYm= NWnJ[3ZFOTBiwsXN NU\4OlkzTE2VTx?= M2LPdYFtdGW4aXH0[ZMhWk:VIHHjZ5VufWyjdHnvckBidmRiQWTQJIRmeGyndHnvckBk[XW|ZXSgZpkhUDKRMh?= M2HtNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wN|YzLz5{NEm2NFM3OjxxYU6=
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MG-63 NGnzcZFHfW6ldHnvckBie3OjeR?= Ml73NVAhyrWP NInuXYtFVVOR M3;jUIZi[2muaYTheIV{KEh{T{KtbY5lfWOnZDDheZRweGijZ4mgZYN1cX[jdHnvci=> M4r6UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wN|YzLz5{NEm2NFM3OjxxYU6=
MC3T3-E1 NF[5XFdHfW6ldHnvckBie3OjeR?= NVHyVIVqOTBiwsXN M1e0TmROW09? NFLFOFFn[WOrbHn0ZZRmeyCKMl:yMYlv\HWlZXSgZZV1d3CqYXf5JIFkfGm4YYTpc44> NUPqTmZzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA{PjJpPkK0PVYxOzZ{PD;hQi=>
PC3 NUHtN|h7U2mwYYPlJIF{e2G7 NYjCRXE{OTByINM1US=> NUDEfYxvTE2VTx?= NIfOOlZ2eHKnZ4XsZZRmeyC2aHWgcIV3\Wy|IH;mJGFOWEtiYX7kJGFESyCyaH;zdIhwenmuYYTpc44> M{fuTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUm0NFQ{Lz5{NUW5OFA1OzxxYU6=
PC3M M2LWbmtqdmG|ZTDhd5NigQ>? MVGxNFAhyrWP MoDsSG1UVw>? MWT1dJJm\3WuYYTld{B1cGVibHX2[Yx{KG:oIFHNVGsh[W6mIFHDR{BxcG:|cHjvdplt[XSrb36= NHS4flg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW5OFA1Oyd-MkW1PVQxPDN:L3G+
PC3 MlThSpVv[3Srb36gZZN{[Xl? MUOxNFAhyrWP MXnEUXNQ MlHHbY5lfWOnczDQTVNMN22WT2KgdIF1cHejeYO= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV7NEC0N{c,OjV3OUSwOFM9N2F-
PC3M NVTRToFjTnWwY4Tpc44h[XO|YYm= NEDaSXQyODBiwsXN NUj4cFcxTE2VTx?= MXnpcoR2[2W|IGDJN2swdVSRUjDwZZRpf2G7cx?= MlfnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUSwOFMoRjJ3NUm0NFQ{RC:jPh?=
PC3 NWHCTolZT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= MnzINVAxKML3TR?= NG[yWVBFVVOR MXfzeZBxemW|c3XzJJBzd2yrZnXyZZRqd25? NGrSXm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW5OFA1Oyd-MkW1PVQxPDN:L3G+
PC3M NILkfZhIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M2W2ZVExOCEEtV2= MV3EUXNQ MYDzeZBxemW|c3XzJJBzd2yrZnXyZZRqd25? MmXrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3OUSwOFMoRjJ3NUm0NFQ{RC:jPh?=
MC3T3-E1 M1:wU2tqdmG|ZTDhd5NigQ>? NHmzNYcyOCEQvF2= MmrhSG1UVw>? NU\0OldZcW6mdXPld{B{cWewaX\pZ4FvfCCDTWDLJIFkfGm4YYTpc44> NVHtOnVYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4PVE5PjZpPkK2PFkyQDZ4PD;hQi=>
MC3T3-E1 M{\kUGdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NFvuUGEyOCEQvF2= M17JdWROW09? MXPpcohq[mm2czDE[ZgucW6mdXPl[EBwe3Snb3LsZZN1KGOnbHyg[IVifGh? MmHTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ6OUG4OlYoRjJ4OEmxPFY3RC:jPh?=
MC3T3-E1 NVHGVVVwTnWwY4Tpc44h[XO|YYm= NGnQNmwyOCEQvF2= MYXEUXNQ MmDQbY5pcWKrdIOgSIV5NWmwZIXj[YQhd3irZHH0bZZmKHO2cnXzdy=> NXLkTHlGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[4PVE5PjZpPkK2PFkyQDZ4PD;hQi=>
BL21 MXHGeY5kfGmxbjDhd5NigQ>? M1vDfFEhfG9iMUCgcYlvew>? MkfnRolv\GmwZzDh[oZqdmm2eTD0c{BpfW2jbjDy[YNwdWKrbnHueEBPNXSncn3pcoFtKEGYST30ZYdo\WRicHjvd5Bpd3K7bHH0[YQhSU2SSzDhcJBp[TGkZYThNYdidW2jMTDlfJBz\XO|ZXSgbY4hTXOlaHXybYNpcWFiY3;sbUBDVDJzIHPlcIx{KGGodHXyJFEhfG9iMUCgcYlveyCkeTDibY9t[XmncjDpcpRmem[ncn;t[ZRzgSxiS3SgQUAxNjVzIN88UU4> NFnpd|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkWxNFYzOid-Mk[1NVA3OjJ:L3G+
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PANC-08-13 NVLid2xsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJcohq[mm2aX;uJI9nKGi3bXHuJHBCVkNvMEitNVMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NEA:KDR5Lkm2OFEh|ryPLh?= NYjUdVd1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF{NUC0M{c,W0GQR1XSQE9iRg>?
786-0 Ml3WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;STY5pcWKrdHnvckBw\iCqdX3hckA4QDZvMDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyIE2gOFgvODd|IN88UU4> NHLZeFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTJ3MESvK|5USU6JRWK8M4E,
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MCF7 NH\xd|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHKdWFKdmirYnn0bY9vKG:oIHj1cYFvKE2FRkegZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFQ6NjV6NkWg{txONg>? Mm\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVEzPTB2Lze+V2FPT0WUPD;hQi=>
T47D NEHMbndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmH1TY5pcWKrdHnvckBw\iCqdX3hckBVPDeGIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTBiPTC0PU44ODB5IN88UU4> M3j2ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOjVyND:nQnNCVkeHUkyvZV4>
YKG-1 NFjsPJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1zYO2lvcGmkaYTpc44hd2ZiaIXtZY4hYUuJLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3MDC9JFQ6Njl2MUKg{txONg>? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVI2ODRxJ{7TRW5ITVJ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pCofilin / Cofilin / detyrosinated alpha tubulin / acetylated alpha tubulin ; 

PubMed: 26431377     


A & B. MCF-7 cells were treated with 100–400 μM of A-769662 for 4-24 hours. Protein was harvested and Western blot analysis was done to determine levels of pAMPK at threonine 172, pCofilin at serine 3, detyrosinated alpha tubulin (glu-tub), and acetylated alpha tubulin (acetyl-tub). C & D. BT-549 cells were treated with 100–400 μM A-769662 for 4–24 hours. Protein was harvested and Western blot analysis was done to determine levels of markers listed above.

p-AMPK / AMPK / p-S6K / S6K / p-ACC ; 

PubMed: 22456226     


Representative immunoblots for p-AMPK, total AMPK, p-S6K, total S6K, p-ACC and actin under control conditions (C) and in response to 50 μM A-769662 at various time points.

26431377 22456226
体内研究 A-769662对正常Sprague Dawley大鼠的短期治疗降低肝脏丙二酰CoA水平,和换气比值,VCO2/VO2,表明整体脂肪酸氧化比率增加。A-769662(30 mg/kg, b.i.d.)治疗ob/ob小鼠,减少PEPCK,G6Pase,和FAS的肝脏表达,降低40%血浆葡萄糖,减少体重增加,并显著降低血浆和肝脏甘油三酯水平。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

96-孔 AMPK 试验:

AMPK活性通过监测SAMS多肽底物(标准试验中为20 mM,附加试验中为100 mM)磷酸化根据前述方案(Anderson et al., 2004)测量。为测定 A-769662诱导的AMPK活化是否可逆,AMP或A-769662与大鼠肝脏AMPK在20倍标准试验浓度下预培养10分钟,然后稀释并测量AMPK活性。
细胞实验:

[3]

- 合并
  • Cell lines: MEF细胞
  • Concentrations: 300 μM
  • Incubation Time: 24小时
  • Method: A-769662处理或未处理的MEF细胞的细胞活性如下进行测定:细胞通过胰蛋白酶化采集,并与0.5 mg/mL RNase 和50 μg/mL碘化丙啶在室温下黑暗中进行培育;细胞活性通过流式细胞术使用FACScanto流式细胞分析仪分析,激发激光波长为488 nm,碘化丙啶荧光检测波长为600 nm。为测定细胞周期各阶段的细胞比例,细胞通过胰蛋白酶化采集,通过离心收集,用PBS洗涤,并在80%乙醇中于-20°C固定过夜。随后,将这些固定的细胞离心以除去固定剂,并于黑暗中,室温下,在包含0.5 mg/mL RNase 和50 μg/mL碘化丙啶的PBS中培养20分钟。流式细胞术分析如上进行。G1,S,和G2期的细胞比例使用MODFIT程序测定。细胞培养照片在指示时间使用结合具有20倍物镜的倒置显微镜的相机拍摄。
    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: Sprague Dawley大鼠
  • Dosages: 30 mg/kg
  • Administration: 腹腔注射给药
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 72 mg/mL (199.78 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5% Tween 80+50% ddH2O
5 mg/ml

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 360.39
化学式

C20H12N2O3S

CAS号 844499-71-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
免费分装抑制剂

AMPK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID