Brefeldin A

For research use only. Not for use in humans.

目录号:S7046 中文名称:布雷菲德菌素A

Brefeldin A  Chemical Structure

CAS No. 20350-15-6

Brefeldin A 作用于HCT 116细胞,抑制内酯抗生素和ATPase,作用于protein transport(蛋白转运)IC50为0.2 μM,诱导癌细胞分化和凋亡。它还能提高同源重组修复效率,是CRISPR-mediated HDR的增强剂。Brefeldin A 也是自噬和线粒体自噬的抑制剂。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 655.27 现货
RMB 571.63 现货
RMB 2235.02 现货
RMB 5490.64 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Brefeldin A 发表文献46篇:

产品安全说明书

ATPase抑制剂选择性比较

生物活性

产品描述 Brefeldin A 作用于HCT 116细胞,抑制内酯抗生素和ATPase,作用于protein transport(蛋白转运)IC50为0.2 μM,诱导癌细胞分化和凋亡。它还能提高同源重组修复效率,是CRISPR-mediated HDR的增强剂。Brefeldin A 也是自噬和线粒体自噬的抑制剂。
靶点
ATPase (HCT 116) [1]
0.2 μM
体外研究

Brefeldin A是一种真菌代谢产物,抑制内质网和高尔基体之间的传输,Brefeldin A导致膜蛋白分布受损。Brefeldin A处理HCT 116人结肠癌细胞,观察到形态学的变化,表示细胞分化。Brefeldin A作用于肿瘤细胞,主要通过诱导分化和凋亡而发挥其细胞毒性作用。[1]20 μg/mL Brefeldin A处理检测条6小时,在10mM Indomethacin 和 30 μM L-NOARG存在时,完全废除Bradykinin诱导的松弛。浓度为1 nM 到 1 mM之间的 20 μg/mL Brefeldin A处理,废除Bradykinin诱导的[Ca2+]i降低。Brefeldin A作用于内皮细胞,对Bradykinin 或 Substance P诱导的[Ca2+]i 提高没有影响。[2] Brefeldin A 不影响GTPS与myr-rARF1的自发磷脂依赖性的结合,但完全废除视网膜等渗提取物(RIE)的催化交换,2 μM Brefeldin A的半抑制浓度为2 μM。Brefeldin A 抑制多种膜转运途径。Brefeldin A作用于高尔基体膜或脑细胞质,抑制ADP-核糖基化因子特定的鸟嘌呤核苷酸交换活性。Brefeldin A完全抑制说明视网膜提取物包含ARF特定的鸟嘌呤核苷酸交换因子。Brefeldin A只部分抑制ADP-核糖基化因子(ARFs)释放的视网膜等渗提取物(RIE)催化的GTPS,即使浓度高达300 μM时。[3] Brefeldin A诱导高尔基体与ER融合。Brefeldin A废除 CERT 抑制剂HPA-12的抑制效果。Brefeldin A处理CHO细胞,使鞘磷脂的合成提高2到3倍。[4]除了B-CLL细胞,Brefeldin A还造成多发性骨髓瘤(U266,NCI-H929),JURKAT,HeLa细胞,白血病(HL60,K562,BJAB),结肠(HT-29),和前列腺,及腺样囊性瘤细胞发生凋亡。25 ng/mL Brefeldin A处理 HF4.9 和 HF28RA 细胞,完全抑制细胞生长,而要完全抑制HF1A3 细胞生长则需要高剂量的Brefeldin A (75 ng/mL) 。50-75 ng/mL Brefeldin A 处理HF1A3, HF4.9 和 HF28RA细胞,24小时内抑制细胞增殖,这种作用存在剂量依赖性,3H-胸甘的渗透几乎完全停止,50 ng/ml处理时,HF1A3, HF4.9 和 HF28RA细胞分别抑制26%, 76%, 87%,75 ng/mL处理时,HF1A3, HF4.9 和 HF28RA细胞分别抑制75%, 87%, 92%。YO-PRO 1/PI检测中,Brefeldin A诱导细胞死亡,这种作用存在剂量依赖性。[5] Brefeldin A可提高同源重组效率,是CRISPR-mediated HDR的增强剂[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC12 MojjSpVv[3Srb36gRZN{[Xl? NH7Ue3QzKM7:TR?= MYixJIg> MYPpcohq[mm2czD0bIUhVC2GT2DBJEgzOMLizszNLU1qdmS3Y3XkJJRz[W6|aXXueEBGWktzL{KgdIhwe3Cqb4L5cIF1cW:wwrC= M1HXNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4M{[zNVkyLz5{NkO2N|E6OTxxYU6=
C2C12 MlLWSpVv[3Srb36gRZN{[Xl? MUWxxsDPxGdxbXy= NHe4OnYyyqCq NYf1WlNY[WKxbHnzbIV{KGO7dH;rbY5mKHKnbHXhd4Uh\nKxbTDDNmMyOiCveX;0eYJmew>? NITmRZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK5NVI4QSd-Mk[yPVEzPzl:L3G+
MEFs WT MVjGeY5kfGmxbjDBd5NigQ>? NEHXSo02yqEQvF2= M2PNS|IxKG2rbh?= NEHRbGlk[XW|ZYOgdoV{cWSnboSg[Y57gW2nczDzeYNpKGG|IF7BS3QuT0[SLDD0c{BlcW[odYPlJIJi[2tidH:geIhmKEWU NXfmb2xORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xPVYxOjNpPkK2NVk3ODJ|PD;hQi=>
MEFs VAMP7 KO M4jxTWZ2dmO2aX;uJGF{e2G7 NITxZmI2yqEQvF2= NFjvR4ozOCCvaX6= M4nLWYNifXOnczDy[ZNq\GWwdDDlcpp6dWW|IIP1Z4gh[XNiTlHHWE1ITlBuIITvJIRq\m[3c3WgZoFkcyC2bzD0bIUhTVJ? NHvSXm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG5OlAzOyd-Mk[xPVYxOjN:L3G+
SMCs NVn5XYxUTnWwY4Tpc44hSXO|YYm= Mn3pNVDDqML3Zz;tcC=> M1\1NFAuOTJiaB?= NF:1SGRFVVOR MX;zbI94eyCjIITy[Y5lKHSxd3Hy[JMh[SCqaXfo[ZIh[2:wY3XueJJifGmxbjDv[kB1cGViRWKvV3IhdmW2d3;yb{BqdiC2aHWgdIVzcW63Y3zlZZIh[XKnYdMg NIC0PWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3NlA5OCd-Mk[xO|IxQDB:L3G+
SMCs NFHhcnZHfW6ldHnvckBCe3OjeR?= NYK1TldxOTEEoNM1[{9udA>? MYWwMVEzKGh? MX;EUXNQ NWWxSnlF[2G3c3XzJIEhfHKjboPp[Y51KEOjMjxCpJJmdGWjc3Wg[pJwdSC2aHWgSXIwW1MEoB?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5MkC4NEc,OjZzN{KwPFA9N2F-
HEMC-1 Mkj4SpVv[3Srb36gRZN{[Xl? NXny[3hoOC5zwrFCuYcwdWx? M1W4clI1yqCq NV;UR3pl[2G3c3XzJIEhcGmpaHXyJIlvcGmkaYTvdpkh\W[oZXP0JI9vKGW6b3P5eI9{cXNidHjhckBvd2OxZHH6c4xm MofSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7N{K3OVkoRjJ3OUeyO|U6RC:jPh?=
HUVEC MYfGeY5kfGmxbjDBd5NigQ>? NFfTdFgyOMLizszN NGP4dYMyyqCq NVOwR4h[TE2VTx?= NWWzO41{[WKxbHnzbIV{KGi7cH;4bYEucW6mdXPl[EBz\WynYYPlJI9nKEGWUDDmdo9uKGGyaXPhcEBidmRiYnHzc4xifGW{YXygd5Vz\mGlZYO= NUL0Zo11RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OVY6QDhpPkK1PVU3QTh6PD;hQi=>
HUVEC M1HYVWZ2dmO2aX;uJGF{e2G7 MXyxNOKh|ryP NYf1PZZ4OcLiaB?= MWfEUXNQ MW\pcoNz\WG|ZYRCpJRp\SCwdX3i[ZIh[W6mIHnueIVve2m2eTDv[kBndHWxcnXzZ4VvfCCjcnXhd{Bme3CnY3nhcIx6KGmwIIDldolvfWOuZXHyJJNx[WOn MmnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NU[5PFgoRjJ3OUW2PVg5RC:jPh?=
Caco-2 Mne5SpVv[3Srb36gRZN{[Xl? NFX3XYYzNjVizszN NYHOOIY5OzBibXnu NWLTcJJ1[XS2ZX71ZZRmeyC2aHWgWGdHNc7{MT3t[YRq[XSnZDDpcoNz\WG|ZTDpckBUTVKWIH\1coN1cW:w NYPlW|JFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OVQ6OzFpPkK1PVU1QTNzPD;hQi=>
NRK M1:1W2Z2dmO2aX;uJGF{e2G7 NFfVTo0zODEkgJXu[{9udA>? M4T3[VTjiIWq MV;EUXNQ MkDRdoV{[3WnczDtbZRwfGmlIIDyc4dz\XO|aX;u NF[0R3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm0PFU5Pid-MkW5OFg2QDZ:L3G+
HeLa M2jTWWZ2dmO2aX;uJGF{e2G7 NF7Bd5kzODEkgJXu[{9udA>? MU[zJIg> NHzhR3RFVVOR M2jYW4lv\HWlZYOgeIhmKGG{dHnmbYNq[WxiYoLlZYsufXBib3[geIhmKEexbHfpJINwdXCuZYi= MkHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NEi1PFYoRjJ3OUS4OVg3RC:jPh?=
COS MV7GeY5kfGmxbjDBd5NigQ>? NV\wbpVMOSEQvHevcYw> MkX3N{Bp MVPjc41xdGW2ZXz5JIRqe3CncoPld{B1cGViQWCtNUB{cWewYX|CpC=> MlvEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7MUW5NFAoRjJ3OUG1PVAxRC:jPh?=
DF1  NF3TUXBHfW6ldHnvckBCe3OjeR?= NYe3V4ZHOcLizszNxsA> MnLROFghcA>? MWDEUXNQ MmLJ[Il{eGW{c3XzxsB1cGViZYjv[4Vvd3W|IFPTS4FtVkGlVEKgdJJwfGWrbh?= MnjjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MEewOVQoRjJ3OEC3NFU1RC:jPh?=
nHDFs  NIDnfnhHfW6ldHnvckBCe3OjeR?= MlLUNeKh|ryPwrC= NF;jNWIzyqCq M2mySZBz\X[nboTzJJRp\SCjc4PlcYJtgSCxZjDjfZRwe2:uaXOgZ49ifCCycn;0[YlveyCxboTvJGdwdGerIH3lcYJz[W6ncx?= NEDCUm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe3NlYyPid-MkW3O|I3OTZ:L3G+
FRT  MX7GeY5kfGmxbjDBd5NigQ>? NEDaN282KM7:Zz;tcC=> NYfIWmdyOsLiaB?= MnS5Zoxw[2u|IITyZYZncWOtaX7nJJRpem:3Z3igeIhmKEexbHfpJINwdXCuZYigZpkhcW6qaXLpeIlv\yCHUj30c{1Id2ypaTD0doFve3CxcoS= NX7NUox7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3OlcyOTVpPkK1O|Y4OTF3PD;hQi=>
FRT  MYjGeY5kfGmxbjDBd5NigQ>? NEDnZ|Y2KM7:Zz;tcC=> NVS3cnFxOsLiaB?= NYjJfHY3eHKndnXueJMhfGinIHnuZ5Jm[XOnIHnuJINt\WG4ZXSg{tEhe3WkdX7peJMhf2inbjDbUoEsZWoEoIfhd{Bz\WS3Y3Xk NIn1flU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe2O|EyPSd-MkW3OlcyOTV:L3G+
HepG2  M1njNGZ2dmO2aX;uJGF{e2G7 MknjNgKBkcL3TdMg NHW0SXIzPCCq Mm\pSG1UVw>? MnvW[IVkemWjc3XzJJRp\SCuZY\lcEBw\iCSWGKgcXJPSQ>? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZzNkW5O{c,OjV4MU[1PVc9N2F-
SMCs NWHnXmRmTnWwY4Tpc44hSXO|YYm= NW[wU2hSOc7:Zz;tUC=> MXWzJIg> NHO3V|li[2O3bYXsZZRmeyCFTmDZNkBxem:2ZXnuJIlvKHSqZTDFVkBkd22yYYL0cYVvfCCjbnSgco8hdG:wZ3XyJINwNWyxY3HsbZpm\CC5aYToJJRp\SCJb3znbUBu[XKtZYNCpC=> M1Xqb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUi5OFI2Lz5{NUW4PVQzPTxxYU6=
OB-6 M2rqTWFxd3C2b4Ppd{BCe3OjeR?= MonpNk44yqEQvF2= M{LSZ|Q5KGh? MXXpcoR2[2W|IHHwc5B1d3Orcx?= NFridGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWzNlQ5OCd-MkW1N|I1QDB:L3G+
iPSC-CMs  MUnGeY5kfGmxbjDBd5NigQ>? NHjTUVA2ODBibnevcYw> Mof6OFghcA>? M1v2WYlv[3KnYYPld{B1cGViaX70[Y5{cXS7IH;mJJRp\SCqaXfo[ZIhdW:kaXzpeJkhVEGPUIOgZZQhfGinIHPvd5Qhd2ZidHjlJIxwf2W{IH3vZoltcXS7IIPw[YNq\XN? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR6OE[2Okc,OjV2OEi2OlY9N2F-
SP-Nluc NYSxfoNlTnWwY4Tpc44hSXO|YYm= M1zZR|UhdWdxbVy= NX7hVWxZPiCq NWrRW49zTE2VT9Mg NHv5empk[XW|ZYOgZY4hcW6lcnXhd4UhcW5icnXwc5J1\XJiYXP0bZZqfHliaX6geIhmKHCjcnHzbZRm NUXRWG9ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzPVI6QThpPkK1N|kzQTl6PD;hQi=>
PEXEL-Nluc M4HwXGZ2dmO2aX;uJGF{e2G7 NGnT[Y42KG2pL33M NEKy[pY3KGh? NE\LPHZFVVORwrC= MnWwZ4F2e2W|IHHuJIlv[3KnYYPlJIlvKHKncH;yeIVzKGGldHn2bZR6KGmwIITo[UBx[XKjc3n0[S=> NHTH[oo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO5Nlk6QCd-MkWzPVI6QTh:L3G+
H1299 M3PoeWZ2dmO2aX;uJGF{e2G7 M1PTU|ExKM7:Zz;tcC=> MWWyOEBp NFK2SVBqdmS3Y3XzJIF2fG:yaHHnfeKh MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN6OEm3NEc,OjV|OEi5O|A9N2F-
MDA-MB-231 NHzKOZRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MUmw5qCUPTBizsznM41N MmjTOFghcA>? MXTFR|UxyqB;IECuNFE3KML3Zz;tUC=> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN3NkW2O{c,OjV|NU[1Olc9N2F-
MDA-MB-231 Mmj3RZBweHSxc3nzJGF{e2G7 MoLMNE4yKM7:Zz;tUC=> NW\LTGQ3PCCq MY\pcoR2[2W|IHHwc5B1d3Orcx?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN3NkW2O{c,OjV|NU[1Olc9N2F-
MDA-MB-231 MoHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjabVUxNjBzL{CuNFUh|rypL33M M{m4eVI1KGh? NIG0SZRqdmO{ZXHz[ZMhfGinIH\yZYN1cW:wIH;mJJN2[i2JMTDj[YxtKGSnYoLpdy=> MojzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|NU[1OlcoRjJ3M{W2OVY4RC:jPh?=
MDA-MB-231 Ml3BRZBweHSxc3nzJGF{e2G7 NGTxRmgxNjB34pETNUDPxGdxbVy= MY[yOEBp M1P1Rolv\HWlZYOgVGFTWCBqcH;sfUBCTFBvcnnic5NmKHCxbInt[ZJie2VvMTmgZ4xm[X[jZ3W= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN3NkW2O{c,OjV|NU[1Olc9N2F-
MDA-MB-231 NY\YfXJrTnWwY4Tpc44hSXO|YYm= NVy0[2Z5OOLCk{WwJO69\y:vTB?= M1:zTVI1KGh? NVvhd2JpcW6qaXLpeJMhfGinIH\vdo1ifGmxbjDv[kA{TCCjbnSgNmQh[2:ub37p[ZM> M{PuZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{W2OVY4Lz5{NUO1OlU3PzxxYU6=
A172 M3m1UWZ2dmO2aX;uJGF{e2G7 M1yxZ|ExyqEQvHevcYw> MVm0xsBp M{PMZ2ROW00EoB?= M4LTe5Jme3WudIOgbY4hfGinIILleJJw\3KjZHWgeJJidnOyb4L0JI9nKG[udX;y[ZNk\W62IHfyZY52dGW| NWrZeGpIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWyN|k2ODdpPkK1NlM6PTB5PD;hQi=>
KMS-6 M1\UZmZ2dmO2aX;uJGF{e2G7 MlywNeKh|ryPwrC= NGr2cmczPCCq MojZ[ZhpcWKrdIOgbIFt\iC2aHWgd4VkemW2aX;uJI9nKGejbHHubY4uVEliYYOg[IllKHSqZTDjc451em:u MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ{OUGyOkc,OjV{MkmxNlY9N2F-
MEC NHLVW4RHfW6ldHnvckBCe3OjeR?= M320OVEh|ryP NF\IZ3QyNjViaB?= M3vSeYNifXOnczDhJIRz[W2jdHnjJIRm[3KnYYPlJIlvKHSqZTDzeZJn[WOnIG\FS2ZTOg>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJ{OEixOUc,OjV{Mki4NVU9N2F-
HEK293/hERG M3WwdmZ2dmO2aX;uJGF{e2G7 NUnRZYJIOTEEoN88US=> MV2xxsBp MXHy[ZN2dHS|IHnuJIEhfGmvZT3k[ZBmdmSnboSgdoVlfWO2aX;uJI1ifHW{ZTDoSXJIKHC{b4TlbY7DqA>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTJzOES2PUc,OjV{MUi0Olk9N2F-
RBE4 M{PjZ2Fxd3C2b4Ppd{BCe3OjeR?= NUP2S3FPOsLizszN MofWN-KBmzJ2wrDo NXTGfFRjcW6mdXPld{BieG:ydH;zbZMhfGmvZTDk[ZBmdmSnboTsfS=> NGnxOos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGyPFAzPSd-MkWxNlgxOjV:L3G+
RBE4 MUDGeY5kfGmxbjDBd5NigQ>? NWTR[WtNOsLizszN MVqz5qCUOjUEoHi= NYPQcIxQcW6lcnXhd4V{KHSqZTDYRnAyKHC{b4TlbY4hdGW4ZXzzJIFnfGW{IEOgZY5lKDcEoHigc4YhfHKnYYTt[Y51 MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF{OECyOUc,OjVzMkiwNlU9N2F-
RBE4 MWTGeY5kfGmxbjDBd5NigQ>? NVfUVWR6OsLizszN NVvLZYxqO+LCk{K0xsBp NXftZo8zcW6lcnXhd4V{KGGldHn2[UBk[XOyYYPlMVEzKGmwIHGgeIlu\S2mZYDlcoRmdnRibXHucoVzyqB? NX\1XIJLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNlgxOjVpPkK1NVI5ODJ3PD;hQi=>
RBE4 NWrqT|lpTnWwY4Tpc44hSXO|YYm= NHzLVXozyqEQvF2= M3\EdVPjiJN{NNMgbC=> M4r3folv[3KnYYPld{B1cGVibHX2[Yx{KG:oIGLPV{B1cW2nLXTldIVv\GWwdHz5 M4D0OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUK4NFI2Lz5{NUGyPFAzPTxxYU6=
RBE4 NHLlToZHfW6ldHnvckBCe3OjeR?= NWjlWm5vOsLizszN MYOz5qCUOjUEoHi= MoS4bY5lfWOnczDhJIRmdGG7ZXSg[IVxdGW2aX;uJI9nKHSqZTDFVkBE[TJtwrDjc451\W62IHH0JFbDqGhib3[gbY5kfWKjdHnvckB{cWewaX\pZ4FvfGy7 M3jH[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUK4NFI2Lz5{NUGyPFAzPTxxYU6=
RBE4 MoHySpVv[3Srb36gRZN{[Xl? M3jIOlLDqM7:TR?= NUjTSo9yO+LCk{K0xsBp MWTpcoR2[2W|IHHuJI93\XKub3HkJI9nKEOjMjxCpIlvKHSqZTDtbZRw[2ixbnTybYEhcW5idHjlJIZqenO2IEdCpIghd2ZiaX7jeYJifGmxbjCodQKBkT{kgJmwMlAxOSliYoX0JGNiOiwEoHzleoVteyCrbjD0bIl{KG:{Z3Hu[Yxt\SCmZXPy[YF{\WRiYX\0[ZIhOTMEoHigc4YhcW6ldXLheIlwdg>? MoSzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMkiwNlUoRjJ3MUK4NFI2RC:jPh?=
Huh-7  MmC3SpVv[3Srb36gRZN{[Xl? NHziWIoy|rypL33M M1TEclPjiJN{NNMgbC=> Mom2bY5kemWjc3XzJJRp\SCuZY\lcEBw\iCDUFWxJIlvKGFidHnt[U1l\XCnbnTlcpQhdWGwbnXy M2HoXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MEK2NVc1Lz5{NUCyOlE4PDxxYU6=
HepG2  NFPnb4hHfW6ldHnvckBCe3OjeR?= NYXwTI95Oc7:Zz;tUC=> NYDBVFg4O+LCk{K0xsBp NWrXN|FwcW6lcnXhd4V{KHSqZTDs[ZZmdCCxZjDBVGUyKGmwIHGgeIlu\S2mZYDlcoRmdnRibXHucoVz MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB{NkG3OEc,OjVyMk[xO|Q9N2F-
H838-LKB1 MlT3SpVv[3Srb36gRZN{[Xl? NI\Vcog{OMLibnevcYw> NGfBW2EyOi9zODDo M{fDV4lv[3KnYYPld{B1cGVicILveIVqdiCuZY\lcJMhd2ZiQnnQxsA> NYLTTIR4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwNVExQDJpPkK1NFEyODh{PD;hQi=>
H838-KDLKB1  MVjGeY5kfGmxbjDBd5NigQ>? MV6zNOKhdmdxbXy= NVy4NGQxOTJxMUigbC=> MVjpcoNz\WG|ZYOgeIhmKHC{b4TlbY4hdGW4ZXzzJI9nKEKrUNMg NEPmUW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxNVA5Oid-MkWwNVExQDJ:L3G+
H838-KDLKB1  MnHKSpVv[3Srb36gRZN{[Xl? M2rJfFMxyqCwZz;tcC=> NHjYdFYyOi9zODDo MX\pcoNz\WG|ZYOgeIhmKGyndnXsd{Bw\iCyaH;zdIhwenmuYYTl[EBmUUZ{zsGgLJBpd3OyaH:t[WlHOs7zKR?= NGi1V2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUCxNVA5Oid-MkWwNVExQDJ:L3G+
3T3-L1 MYXGeY5kfGmxbjDBd5NigQ>? MnflOUDPxGdxbXy= MnH4N|AhdWmw M1LPbo1qdWmlczD0bIUh\W[oZXP0d{Bw\iCrboP1cIlvKGGwZDDjZZV{\XNicn;ieZN1KHCqb4PwbI9zgWyjdHnvckBw\iCDa4SgLHNmeiB2N{OpJIFv\CCyaH;zdIhwenmuYYTpc44hd2ZiQWOxOlAhMFSqcjC2OFIh[W6mIGPldkA2QDhr NEfxPJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0N|gzPyd-MkS4OFM5Ojd:L3G+
3T3-L1 NGnWXYxHfW6ldHnvckBCe3OjeR?= NV31b2ZIPSEQvHevcYw> NUTxNpVWOzBibXnu M{HMdZJm[2GyaYT1cIF1\XNiaX7zeYxqdiCjY4Tpc44hf2m2aDDy[ZNx\WO2IITvJJJm\3WuYYTpcochSWu2IHHjeIl3cXS7IHHu[EBCWzF4MDDwbI9{eGixconsZZRqd25? MmrwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEO4NlcoRjJ2OESzPFI4RC:jPh?=
3T3-L1 NGnLc3ZHfW6ldHnvckBCe3OjeR?= NF\uSWE2KM7:Zz;tcC=> MYCzNEBucW5? MoXZZ4F2e2W|IILleoVze2mkbHWgdoVlcXO2cnnieZRqd25ib3[gS2xWXDR? NHXVSGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0N|gzPyd-MkS4OFM5Ojd:L3G+
3T3-L1 M4jxd2Z2dmO2aX;uJGF{e2G7 NFzjUG82KM7:Zz;tcC=> NIjPUYIyyqCq MVjjZZV{\XNicnXkbZN1emmkdYTpc44hd2ZiR1zVWFQh[nW2IH7veEBqdmO{ZXHz[UBqdiCpbIXjc5NmKHWydHHr[S=> MlLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEO4NlcoRjJ2OESzPFI4RC:jPh?=
3T3-L1 MoHlSpVv[3Srb36gRZN{[Xl? NGnCZVI2KM7:Zz;tcC=> NH63PJoyyqCq MWTjZZV{\XNicHjvd5Bpd3K7bHH0bY9vKG:oIITo[UBHd3iRMTD0doFve2O{aYD0bY9vKG[jY4Tvdi=> NIDpcpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi0N|gzPyd-MkS4OFM5Ojd:L3G+
HeLa  MVzGeY5kfGmxbjDBd5NigQ>? MVy1JO69\y:vbB?= MV2zJIg> MY\jZZV{\XNiboXjcIVieiCneHPseZNqd25ib3[geIhmKE[xeF:xJJRz[W6|Y4LpdJRqd25iZnHjeI9zKGGwZDDk[YNz\WG|ZYOgeJJidnOlcnnweIlwdiCxZjDGc5hQOS2{ZXf1cIF1\WRiZ3Xu[ZM> MlqwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NEO4NlcoRjJ2OESzPFI4RC:jPh?=
HEK293 NUn5SYJtTnWwY4Tpc44hSXO|YYm= M1;4TVXDqM7:Zz;tcC=> MXixNuKhcA>? NU\peYdx[WKxbHnzbIV{KEOPQT3pcoR2[2WmIFPSSWxFOiC|ZXPy[ZRqd25? M4SydVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nki3OFMyLz5{NE[4O|Q{OTxxYU6=
COS-1 MkPySpVv[3Srb36gRZN{[Xl? NVXFOJpHPSEEtXevcYw> MmLaNlQhcMLi MUPy[ZN1emmldIOgcI9k[WyrenH0bY9vKG:oIF7CJIlvKHSqZTDw[ZJqdnWlbHXhdkBz\Werb39CpC=> NFv0WHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[3NVc2OSd-MkS2O|E4PTF:L3G+
PRP M2HjUGZ2dmO2aX;uJGF{e2G7 NXX4d5lQOTBizszN NEnJNnhi[nKxZ3H0[ZMhW0SILUJOtU1u\WSrYYTl[EBEYEOUNzDlfJRmem6jbHn6ZZRqd28EoB?= M160SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Nk[4O|UxLz5{NE[2PFc2ODxxYU6=
RAW264.7 NGC2VIxCeG:ydH;zbZMhSXO|YYm= NUXZfpkzPCEQvF2= MXW0PEBp MoHhZZR1\W63YYTld{B1cGViaX7obYJqfGmxbjDv[kBwgC2ORFytbY5lfWOnZDDhdI9xfG:|aYOgZY5lKHSqZTDmZYNqdGm2YYTpc44hd2ZiY3jvcIV{fGW{b3yg[YZndHW6IHL5JGFkNWiHLUG4RU1PUDJ? NYDlWnNMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|kxOzJpPkK0OlM6ODN{PD;hQi=>
MDMs NXr2[5J3SXCxcITvd4l{KEG|c3H5 M2XpO|Ex6oDLzsznM41t NWKxbZVTOTJxMUWgbC=> MWjpcoR2[2W|IHHwc5B1d3Orcx?= Mnj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3NU[2PVUoRjJ2NUW2Olk2RC:jPh?=
PMHs  NXfZOoR[TnWwY4Tpc44hSXO|YYm= NELEb5cyOOLCk{Kw5qCK|rypL33s MnzQNlTjiImq MYjEUXNQ MUHpcoR2[2WmIFXSJJN1emW|cx?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDRyN{K0Nkc,OjR2MEeyOFI9N2F-
PMHs  MnT4RZBweHSxc3nzJGF{e2G7 Mn7zNVDjiJN{MPMAje69\y:vbB?= NFS5TlIzPOLCiXi= MXPEUXNQ NFfhclFqdmO{ZXHz[ZMh[2WubDDk[YF1cA>? NXLUVGlPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0NFczPDJpPkK0OFA4OjR{PD;hQi=>
HEK293/tau NYPDN286TnWwY4Tpc44hSXO|YYm= MnXQOUDPxE1? M{TMNFEwOi92IHi= NGHHdZRqdmS3Y3XzJGdwdGerIH\yZYdu\W62YYTpc47DqA>? NILNT|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2PFA5QSd-MkSzOlgxQDl:L3G+
HEK293/tau MVjGeY5kfGmxbjDBd5NigQ>? MXO1JO69VQ>? NUn5d3BzOyCq MnnlbY5lfWOnczD0ZZUhcHmyZYLwbI9{eGixconsZZRqd25? NWDs[I9kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlgxQDlpPkK0N|Y5ODh7PD;hQi=>
ADF MW\GeY5kfGmxbjDBd5NigQ>? M1PKblExKM7:TR?= M2\wNFE3KGh? MmLobY5pcWKrdIOgeIhmKFqwQ3yyMYlv\HWlZXSgeJJidnOub3PheIlwdiCxZjDDVnQ> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{OEKzNkc,OjR{MkiyN|I9N2F-
U373  NXX2[3BXTnWwY4Tpc44hSXO|YYm= NF\4RmgyOCEQvF2= MVixOkBp M4G4d4lvcGmkaYTzJJRp\SCcblPsNk1qdmS3Y3XkJJRz[W6|bH;jZZRqd25ib3[gR3JV MoPsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkiyN|IoRjJ2MkK4NlMzRC:jPh?=
RKO-HIPK2i MVrGeY5kfGmxbjDBd5NigQ>? M1O1Z|ExKM7:TR?= MoPKNVYhcA>? NV2xXXAxcW6qaXLpeJMhfGinIGruR4wzNWmwZIXj[YQhfHKjboPsc4NifGmxbjDv[kBEWlR? NFHFe3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyPFI{Oid-MkSyNlgzOzJ:L3G+
ADF  M2fIcWZ2dmO2aX;uJGF{e2G7 M{fUd|ExKM7:TdMg MknyOkBp MXvpcZBicXK|IITo[UBFSyCjY4TpeoF1cW:w NUTjTmg2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlgzOzJpPkK0NlI5OjN{PD;hQi=>
Huh7 NHf6dFVHfW6ldHnvckBCe3OjeR?= MXK1JO69\y:vbB?= M3vmfFQhcA>? M4nid4Fjd2yrc3jld{B1cGVic3XjdoV1cW:wIH;mJEBqdnS{YXPlcIx2dGG{IFHwc2I> NFjSUJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGwNFE1OCd-MkSxNFAyPDB:L3G+
Huh7 NHXuV3lHfW6ldHnvckBCe3OjeR?= M{fMSlUh|rypL33s MUCxJIg> NHLYXItk[XW|ZYOgZUB{cWewaX\pZ4FvfCCrbnPy[YF{\SCrbjDBdI9DNWO{ZYPj[Y51ew>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyMEG0NEc,OjRzMECxOFA9N2F-
Huh7 M1zzPGZ2dmO2aX;uJGF{e2G7 M33BZ|XjiJNzMNMgcocwdWx? NGn1ZZUyOiCq Mo[2bY5kemWjc3XzJGFxd0JvY4Lld4NmdnS|IIfpeIhwfXRiaX7obYJqfGmwZzDz[YNz\XSrb36= M{TqdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUCwNVQxLz5{NEGwNFE1ODxxYU6=
BAECs M1O4UWZ2dmO2aX;uJGF{e2G7 M1zGW|Uh|rypL33s NEXifJgxNTRiaB?= NXPUWpF2cW6mdXPld{B1cGVicnHwbYQh\GWyaH;zdIhwenmuYYTpc44hd2ZiZV7PV{BifCCVZYKxNVc6 M4\WXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEi1NlI2Lz5{NEC4OVIzPTxxYU6=
Macrophages MmjWSpVv[3Srb36gRZN{[Xl? M3TKTFcyKML3TR?= MVK2JIg> NF7vbYFqdmirYnn0d{BtfW6jc3nuJIlvfGW{bnHsbZpifGmxbtMg NUW1cHhRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwN|k4PDBpPkK0NFM6PzRyPD;hQi=>
Colo 205 NG\4[|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDGNE02KM7:Zz;tUC=> NYm0WolRPDhiaB?= MlPzbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5ic4XzdIVve2mxbjDjeYx1fXKnczD3bZRpKGGwIHXzeIlu[XSnZDDJR|UxKG:oII6xOUBv\y:vTB?= MkWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7N{O5PVYoRjJ|OUezPVk3RC:jPh?=
Colo 205 MlXoSpVv[3Srb36gRZN{[Xl? M3qzNlAvODF{LUCuNFI2KM7:Zz;tUC=> MX[xOEBl NHrJPINz\WS3Y3XzJJRp\SClbH;uc4dmdmmlaYT5JI9nKEOxbH:gNlA2KEOVQ4O= MnTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7N{O5PVYoRjJ|OUezPVk3RC:jPh?=
Colo 205 NELidYVCeG:ydH;zbZMhSXO|YYm= Mn6wNE4yKM7:Zz;tUC=> MnjONE0zPCCq MYjpcoR2[2W|IHHwc5B1d3OrczDv[kBEd2yxIEKwOUBk\WyuczDpckB{fXOyZX7zbY9vKGO3bIT1doV{ NEW5SW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m3N|k6Pid-MkO5O|M6QTZ:L3G+
Colo 205 MWjGeY5kfGmxbjDBd5NigQ>? MYGwMlAyPSEQvHevcWw> NFrrNGEzPCCq M{HKOYlv\HWlZYOgeIhmKGW6cILld5Nqd25ib3[gSXIhe3S{ZYPzMZJmdGG2ZXSg[4Vv\XN? M3nSTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUezPVk3Lz5{M{m3N|k6PjxxYU6=
Colo 205 M1T0UWZ2dmO2aX;uJGF{e2G7 NWPh[ZRtOC5yMUWg{txoN22O NX73dnZvOjRiaB?= MXPpcohq[mm2czD0bIUh[WO2aY\peJkhd2ZiTV3Qdy=> MnvGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7N{O5PVYoRjJ|OUezPVk3RC:jPh?=
IBRS2 M{LBNWZ2dmO2aX;uJGF{e2G7 NX\oS4Z1PSEQvHevcYw> NVfBVnlQOC53IHi= MVjEUXNQ M2T3d4Rqe3K3cITzJJRp\SCHUlfJR{BidmRiR3;s[4nDqA>? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4M{WzOEc,OjN7NkO1N|Q9N2F-
IBRS2 MlvLSpVv[3Srb36gRZN{[Xl? NEW1blM2KM7:Zz;tcC=> MYewMlUhcA>? MXzEUXNQ MnjW[Y5p[W6lZYOgSm1FXiCrbn\lZ5Rqd25? NXXneYNbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OlM2OzRpPkKzPVY{PTN2PD;hQi=>
HeLa NUKyVnRoTnWwY4Tpc44hSXO|YYm= Ml3rNkDPxE1? M2PONVIhcMLi MoP1ZZR1\W63YYTld{B1cGViVF7GMYlv\HWlZXSgd4VkemW2aX;uJI9nKEmOLUG1 NX;ET49DRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OVA5QTJpPkKzPVUxQDl{PD;hQi=>
HFS  MYTGeY5kfGmxbjDBd5NigQ>? NIHMSGQxNTFizsznM41t NHiy[|AzPCCq MUfHUHRRKGW6cILld5Nqd25icnXhZ4hmeyCjIIDsZZRm[XViYYSgZ49v[2WwdILheIlwdnNiYYOgcI94KGG|IECuNFEhyrWpL33s NYn0W|E{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4PVQ3OzNpPkKzPFk1PjN|PD;hQi=>
HFS  MYLGeY5kfGmxbjDBd5NigQ>? MmPWNE4xOSEEtXevcYw> MX:yOEBp MonFbY5kemWjc3XzJJRp\SCneIDy[ZN{cW:wIH;mJIdtgWOxc4DobY5od2yrcHnkJJN6dnSqYYPlJIdmdmW|IHH0JFYhcA>? NEfCeoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i5OFY{Oyd-MkO4PVQ3OzN:L3G+
OVCAR-3 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYKx5qCUOTYEoN88UeKh MmTsNlTDqGh? NH3TXFhqdmS3Y3XzJIEhdG:|czDv[kBk\WyuII\pZYJqdGm2eTDkc5NmKGSncHXu[IVvfGy7wrC= NE\Cb3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOlk3PCd-MkO4NlY6PjR:L3G+
OVCAR-3 NGfnTpNHfW6ldHnvckBCe3OjeR?= NHTOSFIy6oDVMUZCpO69VcLi NGDoTmQzPMLiaB?= MlvhbY5lfWOnczDueYNt\WG{IHThcYFo\Q>? MnjxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6Mk[5OlQoRjJ|OEK2PVY1RC:jPh?=
OVCAR-3 MmT3RZBweHSxc3nzJGF{e2G7 Mof2NU0yOMLizszN NYjnUodSPCCq M2HtPYlv\HWlZYOgeIhmKGGldHn2ZZRqd25ib3[gZZBweHSxc3nzMZJmdGG2ZXSgdJJwfGWrboO= M1HIeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEK2PVY1Lz5{M{iyOlk3PDxxYU6=
OVCAR-3 M1q0SmFxd3C2b4Ppd{BCe3OjeR?= MmrsNVDDqM7:TR?= NGnScogzPMLiaB?= NY\QXJNKcW6mdXPld{Bi[3SrdnH0bY9vKG:oIHPhd5Bie2W| NIPG[5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyOlk3PCd-MkO4NlY6PjR:L3G+
OVCAR-3 NIr6VndHfW6ldHnvckBCe3OjeR?= MnPqNgKBmzFywrFOwG0> M{fWTVI1yqCq NIPUOWNqdmS3Y3XzJIRqe3K3cITpc44hd2ZidHjlJI1qfG:laH;u[JJq[WxidILhcpNu\W2kcnHu[UBxd3SnboTpZYw> MkjGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6Mk[5OlQoRjJ|OEK2PVY1RC:jPh?=
OVCAR-3 NGS0fmJHfW6ldHnvckBCe3OjeR?= MY[x5qCUOTEEoN88US=> NInhfFgzPMLiaB?= NETwU|hqdmS3Y3XzJIZwem2jdHnvckBw\iC{ZXHjeIl3\SCxeInn[Y4he3CnY3nldy=> MnHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6Mk[5OlQoRjJ|OEK2PVY1RC:jPh?=
OVCAR-3 MXfGeY5kfGmxbjDBd5NigQ>? M1vDcVHjiJNzMNMg{txO NHXR[2szPMLiaB?= MVHpcohq[mm2czDj[YxtKGGmaHXzbY9vKGGwZDDtbYdz[XSrb36= NWryPW5SRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NlY6PjRpPkKzPFI3QTZ2PD;hQi=>
MKN45 NHLpd|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXNSmZyUUN3MEywMlAxOSEQvHevcYw> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7M{O0Nkc,OjN5OUOzOFI9N2F-
LOVO MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XwcWlEPTB;MD6xNkDPxGdxbXy= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7M{O0Nkc,OjN5OUOzOFI9N2F-
A549 NU\JU2w5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHoO4w6UUN3ME2wMlA1KM7:Zz;tcC=> Mn\3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUOzOFIoRjJ|N{mzN|QzRC:jPh?=
MDA-MB-435 NXnLXYdUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXzJR|UxRDBwMECxJO69\y:vbB?= MnnVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUOzOFIoRjJ|N{mzN|QzRC:jPh?=
HepG2 Ml3yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRDBwMECxJO69\y:vbB?= Mny1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUOzOFIoRjJ|N{mzN|QzRC:jPh?=
HL-60 M{ezemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjtWXBKSzVyPECuNFAyKM7:Zz;tcC=> Mkj0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUOzOFIoRjJ|N{mzN|QzRC:jPh?=
neural precursor cells M33keWZ2dmO2aX;uJIF{e2G7 MoPtTY5pcWKrdHnvckBw\iCwZYXyc5NxcGW{ZTDwdo9tcW[ncnH0bY9vKG:oIH3veZNmKG6ndYLhcEBxemWldYLzc5Ih[2WubIOgZpkhVVSWIHHzd4F6 NX[ze4gxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0NVc3OzFpPkG3OFE4PjNzPD;hQi=>
HeLa NVO4VIxnTnWwY4Tpc44h[XO|YYm= NH;kdI4yODBidV2= MXWyJIhzew>? Mn7ZSIl{eGW{c3nvckBw\iClaYOg[49t\2libXHyb4VzKGKndHHDc3AhcW5iaIXtZY4hUGWOYTDj[YxteyCjdDCxNFAhfU1iZn;yJFIhcHK| MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV4M{O2PUc,OTd3NkOzOlk9N2F-
HeLa M{S4WWZ2dmO2aX;uJIF{e2G7 MmjwNVAxKHWP NYHyTZZoOiCqcoO= MlTMSIl{eGW{c3nvckBw\iClaYOg[49t\2libXHyb4VzKEuGRVygbY4hcHWvYX6gTIVN[SClZXzsd{BifCBzMECgeW0h\m:{IEKgbJJ{ MlLhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3NkOzOlkoRjF5NU[zN|Y6RC:jPh?=
Vero NGfNWmtHfW6ldHnvckBie3OjeR?= NH\TbHoyOCC3Zz;tcC=> M2rWRlUhdWmwcx?= NVTvVotrUW6qaXLpeIlwdiCxZjDBdoYyKGmwIHHmdolk[W5iZ4Ll[Y4hdW:wa3X5JHZmem9iY3XscJMh[XO|ZYPz[YQh[XNicnHwbYQhSVBvMTDkbZNx\XK|YXyg[pJwdSCpb3znbUBu\W2kcnHu[ZMh[XRiMUCgeYcwdWxiYX\0[ZIhPSCvaX7zJIJ6KGmvbYXuc4ZtfW:{ZYPj[Y5k\SCvZYToc4Q> MnzUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzOEK3PFMoRjF7MUiyO|g{RC:jPh?=
Vero MUDGeY5kfGmxbjDhd5NigQ>? Mor0NVAhfWdxbXy= NYnPcYJsPSCvaX7z M3jTfmlvcGmkaYTpc44hd2ZiQYLmNUBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDW[ZJwKGOnbHzzJIF{e2W|c3XkJIF{KHKjcHnkJGdISTNiZHnzdIVze2GuIH\yc40hfHKjboOg[49t\2libnX0e49zcyCjdDCxNEB2\y:vbDDh[pRmeiB3IH3pcpMh[nliaX3teY5w\my3b4Lld4NmdmOnIH3leIhw\A>? M4nWWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUiyO|g{Lz5zOUG4Nlc5OzxxYU6=
Vero NXv4OnQzTnWwY4Tpc44h[XO|YYm= M3HISFExKHWP M3\MXFEhcHJ? MnrPTY5pcWKrdHnvckBw\iCJQl[xJHFPXiCmZXzleIVlKG23dHHueEBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDW[ZJwKGOnbHzzJIF{e2W|c3XkJIF{KGWoZnXjeEBwdiClaHHu[4UhcW5iZ3;s[4khdW:{cHjvcI9ogSCjdDCxNEB2VSCjZoTldkAyKGi{IHL5JIludXWwb3\seY9z\XOlZX7j[UBu\XSqb3S= NYiwXWdFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxPFI4QDNpPkG5NVgzPzh|PD;hQi=>
Vero MYXGeY5kfGmxbjDhd5NigQ>? M2i2[lExKHWP MW[xJIhz NH\mbpdKdmirYnn0bY9vKG:oIFfCSlEhWU6YIITvJGFCSSCvdYThcpQhcW5iYX\ybYNidiCpcnXlckBud26tZYmgWoVzdyClZXzsd{Bie3Onc4Pl[EBieyCnZn\lZ5Qhd25iY3jhcodmKGmwIHfvcIdqKG2xcoDoc4xw\3liYYSgNVAhfU1iYX\0[ZIhOSCqcjDifUBqdW23bn;mcJVwemW|Y3XuZ4UhdWW2aH;k NH;hPFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUG4Nlc5Oyd-MUmxPFI4QDN:L3G+
Vero MoKwSpVv[3Srb36gZZN{[Xl? MmfRNVAhfWdxbXy= M1;Vc|EhcHJ? NGnvSW5KdmirYnn0bY9vKG:oIFHy[lEhcW5iYX\ybYNidiCpcnXlckBud26tZYmgWoVzdyClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDBdoYyNUeWUDDs[ZZmdHNiYYSgNVAhfWdxbXygZYZ1\XJiMTDodi=> NVn1XZc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxPFI4QDNpPkG5NVgzPzh|PD;hQi=>
Vero NF72WZZHfW6ldHnvckBie3OjeR?= MmXPNVAhfU1? M2HBbWlvcGmkaYTpc44hd2ZiR1LGNUBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDW[ZJwKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gV5R5Si2VUzDy[ZRzd2ejZHWgeJJidnOyb4L0JIZzd21iZX7kc5NwdWW|IITvJHRIViCjdDCxNEB2VSCkeTDpcY12dm:obIXvdoV{[2WwY3WgcYV1cG:m M1z6eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUiyO|g{Lz5zOUG4Nlc5OzxxYU6=
Vero NXvuTY16TnWwY4Tpc44h[XO|YYm= NXzXbY51OTBidV2= NV3JVWk{OSCqch?= MVXJcohq[mm2aX;uJI9nKEeERkGgbY4h[W[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDhd5Nme3OnZDDhd{BxfW6ldHH0[UBidmRiZHnm[pV{\SCmaYP0doljfXSrb36gc4YhdWWmaXHsMWdwdGerIH3hdotmeiCpaXHueIlvKG[{b32gWGdPKGG2IEGwJJVOKGGodHXyJFEhcHJiYomgbY1ufW6xZnz1c5Jme2OnbnPlJI1mfGixZB?= NVvyTIJYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxPFI4QDNpPkG5NVgzPzh|PD;hQi=>
Vero NGTl[olHfW6ldHnvckBie3OjeR?= MWCxNEB2\y:vbB?= MWexJIhz NGHaV2JKdmirYnn0bY9vKG:oIFHy[lEhcW5iYX\ybYNidiCpcnXlckBud26tZYmgWoVzdyClZXzsd{Bie3Onc4Pl[EBieyCydX7jeIF1\SCjbnSg[Iln\nW|ZTDkbZN1emmkdYTpc44hd2ZibXXkbYFtNUexbHfpJI1iemuncjDnbYFvfGmwIHH0JFExKHWpL33sJIFnfGW{IEGgbJIh[nliaX3teY5w\my3b4Lld4NmdmOnIH3leIhw\A>? NVLr[nZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmxPFI4QDNpPkG5NVgzPzh|PD;hQi=>
Vero MUnGeY5kfGmxbjDhd5NigQ>? MX2xNEB2\y:vbB?= NHK5OJo2KG2rboO= MUTJcohq[mm2aX;uJI9nKEG{ZkGgbY4h[W[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDhd5Nme3OnZDDhd{Bz[XCrZDDDU3BKKHKnZHnzeJJq[nW2aX;uJIZzd21iZ3;s[4kh[XRiMUCgeYcwdWxiYX\0[ZIhPSCvaX7zJIJ6KGmvbYXuc4ZtfW:{ZYPj[Y5k\SCvZYToc4Q> M2HZVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MUiyO|g{Lz5zOUG4Nlc5OzxxYU6=
Vero NUfySmk5TnWwY4Tpc44h[XO|YYm= MUOxNEB2\y:vbB?= Mm[wNUBpeg>? MUTJcohq[mm2aX;uJI9nKEG{ZkGgbY4h[W[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDhd5Nme3OnZDDhd{B1fWK3bHWg[o9zdWG2aX;uJIZzd21idILhcpMh\2:uZ3mgcoV1f2:{azDhcoQh\W6mb4PvcYV{KGKnZn;y[UBqfHNiZHnzdIVze2GuIHH0JFExKHWpL33sJIFnfGW{IEGgbJIh[nliaX3teY5w\my3b4Lld4NmdmOnIH3leIhw\A>? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF6Mke4N{c,OTlzOEK3PFM9N2F-
Vero MYHGeY5kfGmxbjDhd5NigQ>? NUHXc4VEOTBidXevcYw> MlywNUBpeg>? NECzSHdKdmirYnn0bY9vKG:oIFHy[lEhcW5iYX\ybYNidiCpcnXlckBud26tZYmgWoVzdyClZXzsd{Bie3Onc4Pl[EBieyCpaXHueIlvKHCxc3n0bZZmKHC3bnP0ZZRmKHO2coXjeJVz\XNiaX6gZ49vfGGldDD3bZRpKFOnY{OxMZBwe2m2aY\lJGVTKGW6aYSgd4l1\SCjdDCxNEB2\y:vbDDh[pRmeiBzIHjyJIJ6KGmvbYXuc4ZtfW:{ZYPj[Y5k\SCvZYToc4Q> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF6Mke4N{c,OTlzOEK3PFM9N2F-
Vero MX;GeY5kfGmxbjDhd5NigQ>? NHzOWJMyOCC3Zz;tcC=> NI[xS|hKdmirYnn0bY9vKG:oIFHy[lEhcW5iYX\ybYNidiCpcnXlckBud26tZYmgWoVzdyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHN1gEJvU2OgdoV1em:pYXTlJJRz[W6|cH;yeEBnem:vIHXu[I9{d22nczD0c{BVT05iYYSgNVAhfWdxbXygZpkhcW2vdX7v[ox2d3Knc3PlcoNmKG2ndHjv[C=> MnvzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlzOEK3PFMoRjF7MUiyO|g{RC:jPh?=
Vero NVTOdXhOTnWwY4Tpc44h[XO|YYm= NXP3ZW1tOTBidV2= MUixJIhz MVLJcoR2[3Srb36gc4YhT0KIMTDpckBi\nKrY3HuJIdz\WWwIH3vcotmgSCYZYLvJINmdGy|IHHzd4V{e2WmIHHzJJB2dmO2YYTlJIFv\CCmaX\meZNmKGSrc4TybYJ2fGmxbjDv[kBkcXNvR3;s[4khdWG{a3XyJGdOOTNyIH\yc40hXEeQIHH0JFExKHWPIHHmeIVzKDFiaIKgZpkhcW2vdX7v[ox2d3Knc3PlcoNmKG2ndHjv[C=> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF6Mke4N{c,OTlzOEK3PFM9N2F-
Vero NF7UR49HfW6ldHnvckBie3OjeR?= NELVVWwyOCC3Zz;tcC=> Mn\INUBpeg>? M{fTemlvcGmkaYTpc44hd2ZiQYLmNUBqdiCjZoLpZ4FvKGe{ZXXuJI1wdmuneTDW[ZJwKGOnbHzzJIF{e2W|c3XkJIF{KHC3bnP0ZZRmKGGwZDDkbYZnfXOnIHTpd5RzcWK3dHnvckBw\iClaYOtS49t\2libXHyb4VzKEePMUOwJIF1KDFyIIXnM41tKGGodHXyJFEhcHJiYomgbY1ufW6xZnz1c5Jme2OnbnPlJI1mfGixZB?= NF7TbIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUG4Nlc5Oyd-MUmxPFI4QDN:L3G+
NRK NHX1eYhHfW6ldHnvckBie3OjeR?= MojOO{B2VQ>? NHPvdpU3OCCvaX7z NVPpOlA1T2:uZ3mt[Il{fHW{Ynnu[{Bi[3Srdnn0fUBqdiCpb3znbUBieHCjcnH0eZMhd2ZicnH0JG5TUyClZXzsd{Bie3Onc4Pl[EBieyCodYPpc44hd2ZiZ3;s[4khdWWvYoLhcoUh\nW|aX;uJJdqfGhiZX7kc5Bt[XOvaXOgdoV1cWO3bIXtJIF1KDdidV2gZYZ1\XJiNkCgcYlveyCkeTDIc4VkcHO2IEOzOFIhe3SjaX7pcocu[mG|ZXSgbY1ufW6xZnz1c5Jme2OnbnPlJI1q[3Kxc3PvdJk> MmiwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzOEm4NVMoRjJyMUi5PFE{RC:jPh?=
NCI60 NX\MTmR{S3m2b4P0ZZRq[yCjc4PhfS=> MXrDfZRwe3SjdHnjJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTlPJOlAh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVAvODJyNt88UU4> NYnDU2tORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NFU6PTdpPkKzPFA2QTV5PD;hQi=>
NCI60 M2LHbWN6fG:|dHH0bYMh[XO|YYm= MlPzR5l1d3O2YYTpZ{Bi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF7DTVYxKGOnbHzzJIJ6KFOUQjDhd5NigSxiVFfJQVMvPDkQvF2u NIH3OlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwOVk2Pyd-MkO4NFU6PTd:L3G+
HeLa R19 NUHv[JR[SW62aY\pdoFtKGG|c3H5 NXrrTGRuOC53IIXN NVS2c3pKPyCqcoO= NH6ybG1CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBEd3i|YXPrbYV3cXK3czDCN{Bqdm[nY4Tl[EBqdiCqdX3hckBJ\UyjIGKxPUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKHKncHzpZ4F1cW:wIHH0JFAvPSC3TTDh[pRmeiB5IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= M{ezVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEC1PVU4Lz5{M{iwOVk2PzxxYU6=
HeLa MX3GeY5kfGmxbjDhd5NigQ>? M2KyTlUhfU1? MnHaN|AhfG9iNkCgcYlvew>? M1zlZmlv\HWldHnvckBw\iCpb3znbUBieHCjcnH0eZMh\Gm|YYPz[Y1jdHliaX6gbJVu[W5iSHXMZUBk\WyuczDheEA2KHWPIHHmeIVzKDNyIITvJFYxKG2rboOgZpkh[2:wZn;jZYwhdWmlcn;zZ49xcWNiYX7hcJl{cXN? NEm4SYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iwOVk2Pyd-MkO4NFU6PTd:L3G+
Arabidopsis thaliana root cells MUXGeY5kfGmxbjDhd5NigQ>? MkXXPVAhfU1? NVfwUIRqOzBibXnudy=> NXjLd5V5UW6mdXP0bY9vKG:oIH3vdpBpd2yxZ3njZYwh[2ijbnfld{Bw\iCpb3znbUBieHCjcnH0eZMhcW5iQYLhZolld3C|aYOgeIhidGmjbnGgdo9wfCClZXzsd{BmgHC{ZYPzbY5oKFOWLWnGVE9XUEGjMT3SSnAh[XRiOUCgeW0h[W[2ZYKgN|AhdWmwczDifUBkd26ob3PhcEBt[XOncjDzZ4FvdmmwZzDtbYNzd3Olb4DpZ{BidmGueYPpdy=> NVzPelEyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4NFU6PTdpPkKzPFA2QTV5PD;hQi=>
HeLa R19 NWPBR2VOSW62aY\pdoFtKGG|c3H5 NWi3UJlIPSC2bzC1NEB2VQ>? MVi3JIhzew>? NX7zS2g1SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhS2:6c3Hjb4lmfmm{dYOgRlMhcW6oZXP0[YQhcW5iaIXtZY4hUGWOYTDSNVkh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCC{ZYDsbYNifGmxbjDheEA2KHSxIEWwJJVOKGGodHXyJFchcHK|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigQ>? M3rHR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEC1PVU4Lz5{M{iwOVk2PzxxYU6=
PC3 Mmm1SpVv[3Srb36gZZN{[Xl? NEjCdIQ2OCCwTR?= MlTXO|IhcHK| MoHsVI91\W62aXH0bY9vKG:oIEOgcm0h\G:lZYThfIVtNWmwZIXj[YQh[3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWEN|IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHH0JFUxKG6PIHHmeIVzKDd{IHjyd{BjgSC2conwZY4h[my3ZTDlfINtfXOrb36gZZN{[Xl? NE\VdoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES2Nlg{OSd-Mki0OlI5OzF:L3G+
L02 NG\NZpZEgXSxdH;4bYNqfHliYYPzZZk> M1\0VVczKGi{cx?= NX7lepZoS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVDB{IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRDBwMECwOO69VS5? M1Sy[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEm0NlUyLz5{OES5OFI2OTxxYU6=
PC3 NEjWRZVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M3\yVVczKGi{cx?= NXLBZ5p6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkC2PO69VS5? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR7NEK1NUc,Ojh2OUSyOVE9N2F-
HT-29 MoD5RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M2rYdVczKGi{cx?= M{\1WmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkG2{txONg>? M4qzflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEm0NlUyLz5{OES5OFI2OTxxYU6=
HepG2 M2nrcWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 MnrHO|IhcHK| MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEincFeyJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OC5|Nd88UU4> NXPTbW5ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki0PVQzPTFpPkK4OFk1OjVzPD;hQi=>
LO2 M3\pdGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M3nMSVczKGi{cx?= NETkdZlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzPNkBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRDBwMECx{txONg>? NF;NS3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWyOFczQCd-Mkm1NlQ4Ojh:L3G+
Bel7402 MWrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NGXzR4I4OiCqcoO= NYiwW|JmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB{IHPlcIx{KGGodHXyJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NE4xOjUQvF2u NUXUSWN3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1NlQ4OjhpPkK5OVI1PzJ6PD;hQi=>
HL60 MnnCRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NXzyN3h6PzJiaILz MV3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjB{Nd88UU4> M1HQSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUK0O|I5Lz5{OUWyOFczQDxxYU6=
PC3 NVnEXnA5SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NFPhTZI4OiCqcoO= MWfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCFMzDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvODZ6zszNMi=> M1ruRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUK0O|I5Lz5{OUWyOFczQDxxYU6=
Bel7402/5-FU NHHnNJFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NV74VlRGPzJiaILz NVLnToh6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDC[Yw4PDB{L{WtSnUh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1yLkiy{txONg>? NFfpdGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWyOFczQCd-Mkm1NlQ4Ojh:L3G+
HeLa MUDGeY5kfGmxbjDhd5NigQ>? NF3wO2QyQCC3TR?= MYizJIhzew>? MUTJcohq[mm2aX;uJI9nKGGua3HsbY5mKHCqb4PwbIF1[XOnIIPlZ5JmfGmxbjDpckBpfW2jbjDI[WxiKGOnbHzzJIF1KDF6IIXNJIlv[3WkYYTl[EBnd3JiMzDodpM> MlvsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF2MkG5OlUoRjNzNEKxPVY2RC:jPh?=
VERO-E6 NHPjcY1HfW6ldHnvckBie3OjeR?= M3rE[lQ5KGi{cx?= NFrXeXBF\XSncn3pcoF1cW:wIH;mJGlEPTBidnHseYV{KG[xcjDpcohq[mm2aX;uJI9nKFODUmOtR49XNTJiaX7keYNm\CCleYTveI95cWOrdImgc4YhXkWUTz3FOkBk\WyuczDh[pRmeiB2ODDoc5VzeyBiZYjwc5N2emVidH:gNE4xOSCPT1mgV2FTWyCFb2[tNkB3cXK3czDifUBpcWeqIHPvcpRmdnRiaX3h[4lv\yxiSVO1NF0xNjB{zszNMi=> NUj1eI9YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTl7OECvK|5EcEWPQly8M4E,
VERO-E6 MnW5SpVv[3Srb36gZZN{[Xl? MmjiOFghcHK| M1jPTXRwgGmlaYT5JGNEPTBiYXfhbY5{fCCYRWLPMWU3KGOnbHzzJIRmfGW{bXnu[YQh[XRiNEigbI92enNiYomgbIlocCClb370[Y51KGmvYXfpcochMHOjbXWgZ49v\Gm2aX;ud{BieyB{X1zFXUB4cXSqb4X0JIV5eG:|dYLlJJRwKDBwMEGgUW9KKFODUmOgR49XNTJidnnyeZMqNCCFQ{WwQVAvODcQvF2u MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPVk5OC9pPlPoSW1DVDxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p53 / GRP78 ; 

PubMed: 22859938     


(B) present the dose- and time- dependent effect of ER stress inducer brefeldin A on p53 expression. MCF-7 Cells were exposed to brefeldin A in 10% FBS–supplemented DMEM as time and dose indicated. The cell lysates were analyzed by western blotting with antibodies for p53, GRP78, and α-tubulin. The p53 protein expression level was quantified densitometrically. GRP78 served as an ER stress marker. 

22859938
Immunofluorescence
FMNL1 / GM130 ; 

PubMed: 21868368     


FMNL1 colocalizes with the cis-Golgi marker GM130 and is dispersed by treatment with brefeldin A (+BFA).

ErbB3 / Calnexin ; 

PubMed: 21576364     


293T cells transfected with ErbB3 were treated without (top) or with (bottom) BfA, and confocal immunofluorescence microscopy was used to examine the colocalization of ErbB3 with endogenous calnexin.

MTP / GBF1 ; 

PubMed: 26267806     


3T3-L1 cells, plated as above, were treated with brefeldin A (5 μg/ml, 3 hr), fixed and stained for MTP and GBF1. Brefeldin A treatment leads to redistribution of MTP from the juxtanuclear region, similar to what occurs with GBF1. Bars = 10 μm.

21868368 21576364 26267806
Growth inhibition assay
Cell viability; 

PubMed: 28462831     


Effects of brefeldin A on human prostate cancer cells. LNCaP and PC-3 cells were seeded in cell culture dishes and incubated for 24 h. The cells were then treated with different concentrations of brefeldin A for 72 h. The number of viable cells was determined by the trypan blue exclusion assay. Apoptosis was determined by propidium iodide staining and morphological assessment.

28462831

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[5]

- 合并
  • Cell lines: 人类滤泡性淋巴瘤细胞系HF1A3,HF4.9 和HF28RA
  • Concentrations: 0 ng/mL-75 ng/mL
  • Incubation Time: 5 天
  • Method:

    使用 YO-PRO 1/PI 和 SYTO16/PI 探针对细胞进行双染色,测定HF1A3, HF4.9细胞活力。为了测定细胞增殖使用0–100 ng/mL Brefeldin A 在完全培养基中处理细胞20小时,然后加入1 μCi/mL [methyl-3H]-胸甘在37°C下再处理4小时。使用 Microbeta 计数仪对渗透的放射性胸甘进行闪烁计数。为了测定Brefeldin A治疗的长期效果,细胞按初始浓度105 cells/mL接种,然后使用0-75 ng/mL Brefeldin A处理长达5天。在指定时间,移除细胞样本,通过标准台酚蓝排除法测定存活细胞数。


    (Only for Reference)

溶解度 (25°C)

体外 DMSO 4 mg/mL (14.26 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 280.36
化学式

C16H24O4

CAS号 20350-15-6
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
免费分装抑制剂

ATPase Signaling Pathway Map

相关ATPase产品

Tags: 购买Brefeldin A | Brefeldin A 供应商 | 采购Brefeldin A | Brefeldin A 价格 | Brefeldin A 生产 | 订购Brefeldin A | Brefeldin A 代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID