PD184352 (CI-1040)

For research use only. Not for use in humans.

目录号:S1020

PD184352 (CI-1040) Chemical Structure

CAS No. 212631-79-3

PD184352 (CI-1040) 是一种ATP非竞争性的MEK1/2抑制剂,细胞试验中IC50为17 nM,对MEK1/2的选择性比MEK5高100倍。PD184352 (CI-1040)可选择性地诱导凋亡。Phase 2。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1146.45 现货
RMB 574.72 现货
RMB 972.33 现货
RMB 2212.13 现货
RMB 5505.57 现货
RMB 6314.65 现货
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客户使用Selleck生产的PD184352 (CI-1040)发表文献120篇:

产品安全说明书

MEK抑制剂选择性比较

生物活性

产品描述 PD184352 (CI-1040) 是一种ATP非竞争性的MEK1/2抑制剂,细胞试验中IC50为17 nM,对MEK1/2的选择性比MEK5高100倍。PD184352 (CI-1040)可选择性地诱导凋亡。Phase 2。
特性 首个开始临床开发的MEK抑制剂。
靶点
MEK1 [1]
(Cell-free assay)
MEK2 [1]
(Cell-free assay)
17 nM 17 nM
体外研究

CI-1040治疗使多重肿瘤细胞,包括结肠26,BX-PC3 胰腺癌,A431 子宫颈癌,HT-29结肠癌, ZR-25-1乳腺癌和SKOV-3 卵巢癌细胞中pMAPK 水平降低。CI-1040治疗不抑制Jun激酶,p38 激酶或Akt的磷酸化作用,这表明CI-1040特定作用于MEK。CI-1040对MAPK活化的抑制阻止细胞周期进程,并诱导G1期阻滞。[1] CI-1040抑制MEK1的IC50为0.3 μM,比抑制Swiss 3T3细胞中EGF诱导的ERK2活化所需的浓度高15倍。这些结果表明CI-1040通过抑制MKK1活化,而不是通过阻断MKK1活性对细胞发挥作用。2 nM PD184352抑制Swiss 3T3细胞中50%的MKK1活化,而超过100倍浓度的CI-1040在体外抑制MEK1。PD184352也会抑制Raf催化的MEK1磷酸化,而对Raf催化的髓鞘碱性蛋白磷酸化没有作用。[2]与仅用DMSO处理的细胞相比,CI-1040抑制86%甲状腺乳头状癌(PTC)细胞生长,10μM浓度时导致RET/PTC1重排。CI-1040对PTC细胞(BRAF 突变)表现出有效的抑制作用,GI50为52 nM,但是对RET/PTC1重排型活性较低,GI50 为1.1 μM。[3]一项最近的研究表明CI-1040增加CML 急变期细胞系,K562,和初级慢性期CD34+ CML细胞中BMS-214662的凋亡作用。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PC-3 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPT[ndRUUN3ME2yOE4xPTJ4IN88US=> M4TkZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
VM-CUB-1 MoDrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPCTWM2OD1{Mz63N|QyKM7:TR?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
LC-2-ad NHfwSo5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jIWmlEPTB;MkOuOlE1PyEQvF2= NXzONYoxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
A704 MojjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTJ|Lk[xNFMh|ryP MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
DMS-114 M4n6XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjnN2lKSzVyPUKzMlU4PjVizszN MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
EKVX MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWf5NY41UUN3ME2yN{4{QTB7IN88US=> NWjhPIlsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
SNU-449 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUD3eIdSUUN3ME2yN{4{OjlizszN MnPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
HCC1806 NUXmVlcxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTJ|LkC1PFMh|ryP M4LITFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
CaR-1 MkLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2rLUmlEPTB;MkOuNFIyOyEQvF2= NF61bnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NCI-H441 MkXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmniTWM2OD1{Mj61Olg6KM7:TR?= M1jTblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
TI-73 NVLzOIhUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrp[ZBKSzVyPUKyMlMzQCEQvF2= NEDPR5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
NCI-H1755 M3\je2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlnaTWM2OD1{MT62O|QyKM7:TR?= M1XxN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
A498 NEi1fVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTJzLkWzNVIh|ryP M2XsXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
CFPAC-1 M1HDZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmjDTWM2OD1{MT6zPFU5KM7:TR?= Mn7XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
ChaGo-K-1 MofBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\RZotKSzVyPUKxMlM3OjdizszN MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
KP-4 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGqzZlhKSzVyPUKxMlEyQTZizszN MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
Becker M3XLfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWPtXIV{UUN3ME2yNU4yOTF6IN88US=> NH7Qeo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
UMC-11 NGjqPVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3qzXGlEPTB;MkCuPVE3PCEQvF2= M{LsN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
TE-6 MnXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoX3TWM2OD1{MD64NFY4KM7:TR?= MlvzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
SF539 M3TLd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoKxTWM2OD1{MD61NVczKM7:TR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
FTC-133 M37MfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTJyLkS3NFMh|ryP MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
NCI-H226 NFTyVpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrTZ4lKSzVyPUKwMlQ{QTlizszN M4\uSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
NCI-H1048 M1z1NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zoOGlEPTB;MkCuN|I3PiEQvF2= NHPHfWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
Calu-3 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{TCdGlEPTB;MUmuOFI{PyEQvF2= NETyV2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
SF126 NX7LTodJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3[zSGlEPTB;MUmuN|UxOiEQvF2= NU\ZbVl[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
NTERA-S-cl-D1 NUH2T2tzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPMXnpKSzVyPUG5MlIxODdizszN MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
SW1573 NEK0NnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M33tSGlEPTB;MUmuNVk3QSEQvF2= MlvJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
SNU-387 M4i4dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkGzTWM2OD1zOT6xPFg6KM7:TR?= M1zKdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
ETK-1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUTJR|UxRTF7LkG4Nlch|ryP M{jxWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
HuO9 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml3pTWM2OD1zOT6xN|c6KM7:TR?= NVfsR5ZsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
RO82-W-1 NIXTXHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY\JR|UxRTF6LkiyPFgh|ryP M4L1flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
D-502MG MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX35PFVtUUN3ME2xPE45OTN7IN88US=> M3TUPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
LS-123 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn74TWM2OD1zOD63PVAyKM7:TR?= MkTqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
OC-314 M4jlXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoK3TWM2OD1zOD63N|g1KM7:TR?= MoTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
Ramos-2G6-4C10 MoXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXzWI9wUUN3ME2xPE42QTJ5IN88US=> Ml3TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
SW962 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NICyenVKSzVyPUG4MlU4ODJizszN NFzZR4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
SW982 NYnKS2gzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrFTWM2OD1zOD61OlQ6KM7:TR?= NXe3OpdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
KYSE-410 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX2wc|JjUUN3ME2xPE41PTd5IN88US=> M3fZdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
HT-3 NFHSVHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\KTWM2OD1zOD6zPVc4KM7:TR?= NYrjR|BERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
CAL-85-1 NXHEV5prT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYDocFdHUUN3ME2xPE4yQDB7IN88US=> M2O0SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
BB30-HNC MmC5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zqdWlEPTB;MUiuNFMyQCEQvF2= MlvGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
D-247MG NEjCVY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTF5LkmyOFch|ryP NFf0SZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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T-24 NYHwTIFRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;qSopKSzVyPUKuPVEyOTJizszN NF3rRnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
CAL-39 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3SzW2lEPTB;Mj64PVk6QSEQvF2= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
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HCC2218 MmTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIrqWpVKSzVyPUKuOlQ4PzhizszN NETGNpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
DB NHHldXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PjNmlEPTB;Mj61PFc5QCEQvF2= NHfkUpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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KNS-62 NHLLRY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFfyZm1KSzVyPUKuOVEyPjRizszN NITVSmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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BB65-RCC MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vNNmlEPTB;Mj60NFY4PCEQvF2= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
CAL-27 Mlq1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jZPWlEPTB;Mj6zPVI{PiEQvF2= Mm\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
DOK NX7wWoNjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfBTWM2OD1{LkO1O|c{KM7:TR?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
NB7 NVWwb|VkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2C1Z2lEPTB;Mj6zOFA5PiEQvF2= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
A2058 MlXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\6XmlEPTB;Mj6xPVI4QSEQvF2= NHrF[Y09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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RXF393 M{PZOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFjBeppKSzVyPUGuPVc5QDdizszN MnfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
LCLC-97TM1 MknRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzOTWM2OD1zLkmyNlU3KM7:TR?= M4DPZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
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BPH-1 MnrVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DV[mlEPTB;MT64PVU6OSEQvF2= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
LOXIMVI MmH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrDbolKSzVyPUGuPFk1OjdizszN MnP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
NCI-H292 NFnMPY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFj0SpFKSzVyPUGuPFY3QTlizszN MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
NB69 MkPWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkWyTWM2OD1zLke4OFY6KM7:TR?= NW\EcZZvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
OVCAR-5 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjhTWM2OD1zLke3Olg4KM7:TR?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
COLO-829 Mlm2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3wTWM2OD1zLk[yNlMzKM7:TR?= NY[wRnVjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
RT-112 MlPPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoX0TWM2OD1zLkW4PVUzKM7:TR?= MkC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA2PDR{Lze+V2FPT0WUPD;hQi=>
SK-MEL-2 M3PiUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TzeWlEPTB;MT61O|Q2QSEQvF2= NHnH[IQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODV2NEKvK|5USU6JRWK8M4E,
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SW1116 NEfEW3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUez[YtrUUN3ME2xMlU3Ozl6IN88US=> M{H6UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
NCI-H1437 M4Xv[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTFwNUWyO|Uh|ryP M4DCblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPTR2Mj:nQnNCVkeHUkyvZV4>
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HO-1-N-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVOzdZMxUUN3ME2xMlUyPjN2IN88US=> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFU1PDJxJ{7TRW5ITVJ:L3G+
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LS-513 Mm\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXzJR|UxRTFwNEWxPVIh|ryP NXXC[m9nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
BV-173 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{jzXmlEPTB;MT60OFAyPSEQvF2= NXzlbZpNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB3NESyM{c,W0GQR1XSQE9iRg>?
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U937 NXjITJRVS2WubDDjfYNt\SCjc4PhfS=> Mkf1NlQhcHK| NYj2VGJNS2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hXTl|NzDj[YxteyCjc4Pld5Nm\CCjczDhZ4N2dXWuYYTpc44h[XRiR{KvUU1xcGG|ZTDh[pRmeiB{NDDodpMh[nlicILvdIllcXWvIHnv[Ill\SC|dHHpcolv\y2kYYPl[EBndG:5IHP5eI9u\XS{aXOgZY5idHm|aYOgbY4heHKnc3XuZ4Uhd2ZiTVXLJIlvcGmkaYTvdkBRTDF6NEO1Ni=> M3Tp[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEe0PVg2Lz5{MkC3OFk5PTxxYU6=
DAOY NWO3fWtueUiWUzDhd5NigQ>? M{LwPJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCGQV;ZJINmdGy| NHj3NZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SJ-GBM2 NWfnWHJjeUiWUzDhd5NigQ>? Mlu2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= NVrUWmJMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
A673 MnXSdWhVWyCjc4PhfS=> MlnLdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEF4N{OgZ4VtdHN? NXvmW5JzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC NFfXV3ZyUFSVIHHzd4F6 MXnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= NFW1S3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-37 MXHxTHRUKGG|c3H5 NULiVYdCeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz NFP5Vnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 MmrudWhVWyCjc4PhfS=> MlXwdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
U-2 OS M3naW5FJXFNiYYPzZZk> NWfCfJZneUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGWtNkBQWyClZXzsdy=> NXPFW4p2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 NXvOOnVUeUiWUzDhd5NigQ>? MYTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy| MkDmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH M33XOZFJXFNiYYPzZZk> NHnYOVFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=> M2flc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 NUfpfJdieUiWUzDhd5NigQ>? MWjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| M1PFd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5 MlnmdWhVWyCjc4PhfS=> NGP0ZppyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTFHOMVUh[2WubIO= M3zwNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-12 NXXZTWlNeUiWUzDhd5NigQ>? NGTzU4dyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
OHS-50 M3\2VZFJXFNiYYPzZZk> Mn3IdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= NHPEXYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
RD MlzkdWhVWyCjc4PhfS=> NETGSIxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUlSgZ4VtdHN? NF3kSoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) NHPTXlByUFSVIHHzd4F6 NXrvcYpkeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF3HJFY{KCh4LWTHJHIqKGOnbHzz NVr5O3VYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
786-0 MYLGeY5kfGmxbjDhd5NigQ>? NU\uO|VVOjRiaILz MW\Jcohq[mm2aX;uJI9nKEONMjDpckBpfW2jbjC3PFYuOCClZXzsd{Bie3Onc4Pl[EBieyCmZXPy[YF{\SCrbjDBb5QyKHCqb4PwbI9zgWyjdHnvckBifCCVZYKxNlkh[W[2ZYKgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqeyxiRVO1NF0xNjIQvF2= M4T6fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNki5PVQ3Lz5|ME[4PVk1PjxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-ERK1/2 / ERK1/2 ; 

PubMed: 21762482     


Effect of PD184352 on phosphorylation of ERK1/2 in sympathetic neurons. Immunoblots were performed with whole cell extracts from sympathetic neurons maintained in the presence of NGF and left untreated or treated with PD184352 at 0.1, 1, 2 or 10 μM for 16 hours. Antibodies to detect total ERK1/2 and phospho-ERK1/2 were used. 

p-ERK5 / ERK5 ; 

PubMed: 21762482     


Effect of PD184352 on phosphorylation of ERK5 in sympathetic neurons.

21762482
Growth inhibition assay
Cell viability; 

PubMed: 29989268     


Relative viability of DU145CR cells treated with PD184352 was significantly less than that of DU145 cells in WST assay. 

29989268
体内研究 CI-1040口服给药减弱小鼠和人结肠肿瘤异种移植物的生长,具有48-200 mg/kg每剂的广泛剂量范围,但是对P388白血病没有作用。[1] CI-1040口服给药(300 mg/kg/d)3周后,抑制来自PTC细胞的肿瘤异种移植物,与未处理的(仅载体处理)小鼠相比,使携带BRAF突变型的移植物减少31.3%,携带RET/PTC1重排型的移植物减少47.5%。小鼠用CI-1040处理时,没有观察到毒性作用。[2]乳腺肿瘤对CI-1040和UCN-01的瞬时暴露引起肿瘤细胞体内死亡,并延长对肿瘤再生长的抑制。CI-1040 (25 mg/kg) 和UCN-01 (0.1-0.2 mg/kg)的联合治疗显著减少MDA-MB-231,并很大程度上废除植入无胸腺小鼠的MCF7肿瘤生长,而任何单一治疗都没有显著活性。联合用药引起显著的肿瘤细胞死亡,这与ERK1/2的磷酸化和Ki67与CD31的免疫活性降低相一致。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[2]

- 合并

MEK1 试验:

MAP激酶被MEK磷酸化后活化;活化的MAP激酶随后使髓鞘碱性蛋白(MBP)磷酸化。32P整合到髓鞘碱性蛋白(MBP)的测定在包含44-kDa MAPK (GST-MAPK)或45-kDa MEK (GST-MEK1)的谷胱甘肽S-转移酶(GST)融合蛋白存在下进行。试验在包含10 μg GST-MEK1,0.5 μg GST-MAPK,和40 μg MBP的50μL 50 mM Tris,pH 7.4/10 mM MgCl2 /2 mM EGTA/10 μM [γ-32P]ATP中进行。30°C下培养15分钟后,加入Laemmli SDS样品缓冲液停止反应。磷酸化的MBP通过SDS/10% PAGE溶解。这种筛选作用使几种小分子MEK抑制剂被发现,例如CI-1040。评估加入顺序的实验表明CI-1040直接抑制MEK1,50%抑制浓度(IC50)为17 nM,而不影响MAPK活性。
细胞实验:

[1]

- 合并
  • Cell lines: Colon 26肿瘤细胞
  • Concentrations: 0.1-10 μM
  • Incubation Time: 24小时
  • Method: 将细胞接种在T-75 cm 2烧瓶,第二天用DMSO或CI-1040处理24小时。收集单细胞悬浮液,并且将沉淀在冰乙醇 (70%)中固定30分钟。样品离心后,将碘化丙啶(50μg/mL)和RNase (30 units/mL)加入沉淀物中,37℃下培养20分钟。过滤后,样品通过流式细胞仪分析。
    (Only for Reference)
动物实验:

[3]

- 合并
  • Animal Models: 无胸腺小鼠的PTC细胞
  • Dosages: 150 mg/kg
  • Administration: 口服,一天两次
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 96 mg/mL (200.55 mM)
Ethanol 14 mg/mL (29.24 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
30% PEG400+0.5% Tween80+5% propylene glycol, pH 9
10 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 478.67
化学式

C17H14ClF2IN2O2

CAS号 212631-79-3
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
免费分装抑制剂

MEK Signaling Pathway Map

MEK Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID