PD98059

For research use only. Not for use in humans.

目录号:S1177

PD98059 Chemical Structure

CAS No. 167869-21-8

PD98059 是一种非ATP竞争性的MEK抑制剂,无细胞试验中IC50为2 μM,特异性抑制MEK-1介导的MAPK激活;不直接抑制ERK1或ERK2。PD98059 是一种aryl hydrocarbon receptor (AHR)的配体并起到拮抗剂的功能。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1099.68 现货
RMB 1213.08 现货
RMB 3845.73 现货
RMB 5489.52 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的PD98059发表文献353篇:

产品安全说明书

MEK抑制剂选择性比较

生物活性

产品描述 PD98059 是一种非ATP竞争性的MEK抑制剂,无细胞试验中IC50为2 μM,特异性抑制MEK-1介导的MAPK激活;不直接抑制ERK1或ERK2。PD98059 是一种aryl hydrocarbon receptor (AHR)的配体并起到拮抗剂的功能。
特性 PD98059不会抑制已经被c-Raf磷酸化的 MEK1
靶点
AhR [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
1 μM 2 μM
体外研究

PD98059 要么抑制本底的MEK1,要么抑制一种被部分激活的MEK突变体,该突变体是 218 和 222号位上丝氨酸突变为谷氨酸后形成的(MEK-2E), IC50 为2 μM. PD98059 不会抑制JNK和P38这两个 MAPK 的同系物. PD98059会高度选择性的抑制MEK, 因为对其它包括Raf 激酶, cAMP-依赖性激酶, 蛋白激酶C, v-Src, 表皮生长因子 (EGF) 受体激酶, 胰岛素受体激酶, PDGF 受体激酶, 和磷脂酰肌醇(-3)激酶等在内的一系列激酶都没有抑制性。 PD98059 会抑制经过PDGF刺激而活化的MAPK 和胸苷 进入 3T3 细胞, IC50分别为 ~10 μM 和 ~7 μM [1]。PD98059可以有效地阻止 MEK1 被Raf 或者MEK 激酶活化, IC50为4 μM, 可以微弱地 抑制MEK2被 Raf 活化, IC50 为50 μM。 在KB 和 PC12 细胞中, PD98059 不会抑制MKK4 和RK激酶这两个参与压力和白细胞介素-1介导的激酶级联反应的MEK的同系物的活化,在Swiss 3T3 细胞中也不会通过胰岛素或者表皮生长因子抑制p70 S6 激酶的活化[2]。PD98059会在不改变细胞生存能力的情况下完全阻断 神经生长因子(NGF)诱导的 PC12 细胞的分化[3] 。PD98059会以剂量依赖性的方式抑制培养在含有破骨细胞分化因子培养基中的 RAW264.7细胞的增值,进而导致TRAP阳性细胞数量明显下降[6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780  NWDrRYhLTnWwY4Tpc44hSXO|YYm= M4DPfFIxyqEQvF2= MnLuNUBp NUPCPJh{[myxY3vzJGRVS0RvaX7keYNm\CCGUkWg[ZhxemW|c3nvci=> M4XTOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Nkm2PFYzLz5{M{[5Olg3OjxxYU6=
AML 1# M3O5WmFxd3C2b4Ppd{BCe3OjeR?= NHXVNJczOMLizszN Mn7kNVIhcA>? MYPlcohidmOnczDj[YxtKGGyb4D0c5NqeyCrbnT1Z4VlKGK7IGOx M4\lelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C2OlkyLz5{M{ewOlY6OTxxYU6=
KG-1  NFnrd49CeG:ydH;zbZMhSXO|YYm= NYTj[JZuOjEEoN88US=> NVvabpJVOTJiaB?= Mn\X[Y5p[W6lZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDTNS=> MoHRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5ME[2PVEoRjJ|N{C2OlkyRC:jPh?=
HUVECs NY\JUWZmSXCxcITvd4l{KEG|c3H5 MUeyM|Qh|ryP NGjBe2EzPC92ODDo NYHme25lcW6mdXPld{Bk\WyuIHTlZZRp MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{WyNEc,OjN5MEe1NlA9N2F-
HepG2  NWSxWo01TnWwY4Tpc44hSXO|YYm= NWDvPVBDOjEEoN88US=> NFTDU4EzPCCq M333RYlvcGmkaYTzJJRp\SCKTz2xJJBzd3SnaX6g[ZhxemW|c3nvckBkdy22cnXheIVlKHerdHigcYV1\m:{bXnu NGq0PWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO|YxQSd-MkO3NFc3ODl:L3G+
NB4  NVe1SmZFSXCxcITvd4l{KEG|c3H5 Mo\6NVAwOjBxNkCg{txO NV7ucFhROS53IHi= NI[ydWFFVVOR M4PEVIRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDjc{11emWjdHXkJJdqfGhiUHHjcIl1[XinbB?= NYLvbohCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3N|U2PDFpPkKzO|M2PTRzPD;hQi=>
BxPC-3 cells NF;4dXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWKyNOKh|ryP MXuwMlUhcA>? NHGwXVFqdmirYnn0d{BXTUeILVGtdoVofWyjdHXkJGhWXkWFIHfyc5d1cCCjbnSgeJVj\SCob4LtZZRqd25iaX7keYNm\CCkeTDQRXIuOiCDUB?= NF\sVYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e2OFA1Pid-MkO3OlQxPDZ:L3G+
MKN45 NI\V[ZNCeG:ydH;zbZMhSXO|YYm= M3\BZVExKM7:TdMg NFrrelAzPCCq MYjpcoNz\WG|ZYOgeIhmKESDUGStbY5lfWOnZDDj[YxtKGGyb4D0c5Nqew>? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7MkW4PEc,OjN5OUK1PFg9N2F-
SGC7901 MVrBdI9xfG:|aYOgRZN{[Xl? M3G5[VExKM7:TdMg NWiwWmtvOjRiaB?= M4ezdYlv[3KnYYPld{B1cGViRFHQWE1qdmS3Y3XkJINmdGxiYYDvdJRwe2m| Mmq4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
MKN45 Ml\pSpVv[3Srb36gRZN{[Xl? NFLBb4wyOCEQvF5CpC=> NY\YOZViOjRiaB?= M4f2[olvcGmkaYTzJJRp\SCneIDy[ZN{cW:wIH;mJJBpd3OyaH;yfYxifGWmIFXST|EwOg>? MlXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
SGC7901 NUjXN5lxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXKxNEDPxE4EoB?= MmO3NlQwPDhxN{KgbC=> NF7rUFRqdmirYnn0d{Bk\WyuIHfyc5d1cCClbz30doVifGWmIIfpeIghTEGSVB?= NH\jUZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlU5QCd-MkO3PVI2QDh:L3G+
MKN45 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\SNVAh|ryPwrC= Mm[2NlQwPDhxN{KgbC=> M{fNZ4lvcGmkaYTzJINmdGxiZ4Lve5RpKGOxLYTy[YF1\WRid3n0bEBFSVCW M3[3dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{myOVg5Lz5{M{e5NlU5QDxxYU6=
SGC7901 M2TzR2Z2dmO2aX;uJGF{e2G7 NIC4UIoyOCEQvF5CpC=> MmjDNlQhcA>? M{jiUYlvcGmkaYTzJJRp\SCneIDy[ZN{cW:wIH;mJJBpd3OyaH;yfYxifGWmIFXST|EwOg>? MnzSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK1PFgoRjJ|N{myOVg5RC:jPh?=
HUASMCs MofoSpVv[3Srb36gRZN{[Xl? MnPONVAh|ryPwrC= MlTLNlQhcA>? MWfkbY1qdmm|aHXzJGFv\yCLST3jZZV{\WRiU1;DV|MhdVKQQTDhcoQheHKxdHXpckBmgHC{ZYPzbY9vyqB? Mlj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MU[0OlgoRjJ|OEG2OFY5RC:jPh?=
HUASMCs MVLGeY5kfGmxbjDBd5NigQ>? M{jLXFExKM7:TdMg M1:0dlI1KGh? NFfNbJBqdmirYnn0d{BCdmdiSVmtbY5lfWOnZDDFVmsyNzJicHjvd5Bpd3K7bHH0bY9vKGyndnXs M1vsZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEG2OFY5Lz5{M{ixOlQ3QDxxYU6=
EPOR/CR3 MnzKSpVv[3Srb36gRZN{[Xl? MlTOOVDDqM7:TR?= M1PtN|MhcA>? NGDqR3pz\WS3Y3XzJGVRVyCjbnSvc5IhUUxvMz3pcoR2[2WmIITo[UB1gXKxc3nu[UBxcG:|cHjvdplt[XSrb39CpC=> NHHCS249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyNFc{OSd-MkO4NlA4OzF:L3G+
NB4  MWPGeY5kfGmxbjDBd5NigQ>? NGqzbm8yOCEQvF5CpC=> NXvpXVh7PzJiaB?= MlnySG1UVw>? MoTubY5pcWKrdIOg[IF{[XSrbnniMYlv\HWlZXSgR2QyOWJiZYjwdoV{e2mxbh?= Mn\iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MkW1PFUoRjJ|OEK1OVg2RC:jPh?=
HL60  NXHjb|RNTnWwY4Tpc44hSXO|YYm= M4Ox[VIxyqEQvF2= MmDCO|IhcA>? MXfEUXNQ MVPpcohq[mm2czDkZZNifGmwaXKtbY5lfWOnZDDDSFEy[iCneIDy[ZN{cW:w MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{NUW4OUc,OjN6MkW1PFU9N2F-
HUVECs MoK5SpVv[3Srb36gRZN{[Xl? MUGyOUDPxE1? NHzhZnAyKGh? NVzw[IdCcW6lcnXhd4UhVkZvzsrCJJA3PSCwdXPs[YFzKHS{YX7zcI9k[XSrb36= MlTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEGwNFgoRjJ|OUCxNFA5RC:jPh?=
LNCaP  NF7FWohHfW6ldHnvckBCe3OjeR?= MWOxNEDPxE4EoB?= MmPVNUBp MXXk[YNz\WG|ZYOgeIhmKEWJRjD1dJJm\3WuYYTl[EBxNVmELUG= Mn;KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6M{izNVgoRjJ|OEO4N|E5RC:jPh?=
HL-60 MojVRZBweHSxc3nzJGF{e2G7 M2rDSVUxyqEQvF2= MVKxJIg> M13CfZJme2O3ZYOgRmEyPDVibXXkbYF1\WRiYYDvdJRwe2m| Mkj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7NEi3OVEoRjJ|OUS4O|UyRC:jPh?=
HepG2 NV\yNG81TnWwY4Tpc44hSXO|YYm= MUm0NEDPxE1? NWXTOmY2Pi9zMjDo MkL2bY5pcWKrdIOgeIhmKGmwY4LlZZNmKG:oIICtSXJMOSCjbnSgdE1kNUq3bjDwdo91\WmwIHX4dJJme3Orb36gZpkhWEx? NVrGfGZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OFI5PTFpPkKzPVQzQDVzPD;hQi=>
HPAF-II NY\Qc2JzTnWwY4Tpc44hSXO|YYm= M133b|ExKM7:TdMg NXntb3YxPiCq NWDLcHV5cW6lcnXhd4UhdWmULUG0N{BmgHC{ZYPzbY9v MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl5M{exNEc,OjN7N{O3NVA9N2F-
BxPC-3 MnzkSpVv[3Srb36gRZN{[Xl? NYPV[3FIOTBizszNxsA> MlywOkBp MYnpcoNz\WG|ZTDtbXIuOTR|IHX4dJJme3Orb36= M4S0UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUezO|ExLz5{M{m3N|cyODxxYU6=
COLO205 NX7Ke5FJSXCxcITvd4l{KEG|c3H5 NG\zOpcyOC9{MD:0NEDPxE1? NHLnR5UzPCCq NWOyR5N4cW6mdXPld{BFVkFibHHk[IVzKG[xcn3heIlwdg>? M164OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG5NVA5Lz5{NECxPVExQDxxYU6=
G292  M13aZ2Fxd3C2b4Ppd{BCe3OjeR?= MoXKN|DDqM7:TR?= Mo\KNkBp M2[0WZJme3SxcnXzJINieHOjaXPpck1qdmS3Y3XkJINmdGxiZHXheIg> M3TBS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEGyPVMxLz5{NECxNlk{ODxxYU6=
SGC7901 MWHBdI9xfG:|aYOgRZN{[Xl? MVOyNOKh|ryP MXqxJIg> MljzbY5pcWKrdIOgZZBweHSxc3nzJIlv\HWlZXSgZpkhUU[QLd8xxsBidmRiNfMAtk1FTlWU NYTpUnRFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNlc4PTBpPkK0NFI4PzVyPD;hQi=>
MGC803  MXTBdI9xfG:|aYOgRZN{[Xl? NXn2Xmp{OjEEoN88US=> M1:3WFEhcA>? MVHpcohq[mm2czDhdI9xfG:|aYOgbY5lfWOnZDDifUBKTk5vzsJCpIFv\CB34pEyMWRHXVJ? NIP6Rog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECyO|c2OCd-MkSwNlc4PTB:L3G+
HPMC M2LtVWFxd3C2b4Ppd{BCe3OjeR?= MXKxNOKh|ryP MlG2NlTDqGh? NVP6V2VTemW4ZYLz[ZMh\GWlcnXhd4UhcW5iY3XscEB3cWGkaXzpeJkhcW6mdXPl[EBjgSCKR2DEVy=> M4nCbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MESyPFM5Lz5{NEC0Nlg{QDxxYU6=
HCT-8 NFvYPGNCeG:ydH;zbZMhSXO|YYm= M4[4fVExyqEQvF2= NUPTZ3VDOjUEoHi= NWOxe3dN\GWlcnXhd4V{KGOnbHygZZBweHSxc3nzJIlv\HWlZXSgZpkhPS2IVR?= NYriUGM6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVU5PjNpPkK0NFk2QDZ|PD;hQi=>
HPMC M1rOTmZ2dmO2aX;uJGF{e2G7 NH33OlAyOMLizszN NGrFb4k1QCCq MmjtdoV3\XK|ZYOgeIhmKGOqYX7n[ZMhcW5iY3XscEBud3KyaH;sc4d6KGmwZIXj[YQh[nliSFfQSHM> M1zmVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MESyPFM5Lz5{NEC0Nlg{QDxxYU6=
A549 MYjGeY5kfGmxbjDBd5NigQ>? MkfCOVDDqM7:TR?= NF;LcngzKGh? MXXicI9kc3NiRWLLJJBpd3OyaH;yfYxifGmxbjDt[YRq[XSnZDDifUAyNDJvTmG= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB4N{eyO{c,OjRyNke3Nlc9N2F-
Caco-2  MnPXRZBweHSxc3nzJGF{e2G7 MXmxNOKh|ryP Ml7qNlTDqGh? M{fLbIRm[3KnYYPld{Bk\WyuIHHwc5B1d3OrczDpcoR2[2WmIHL5JFUuTlV? NF3mZYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5OVg3Oyd-MkSwPVU5PjN:L3G+
AGS NWHxfZVNTnWwY4Tpc44hSXO|YYm= MUSxNEDPxE4EoB?= M3\ZNlAvPSCq MlnTbY5pcWKrdIOgeIhmKHWycnXneYxifGmxbjDv[kB1cGViSVytPEBo\W6n M3S3fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUC2NVY3Lz5{NEGwOlE3PjxxYU6=
Ca9-22 NF;zVoZHfW6ldHnvckBCe3OjeR?= MmLYN{DPxE1? MU[xM|IhcA>? MX3y[YR2[2W|IFjiVk1qdmS3Y3XkJGFVTi1{IIDoc5NxcG:{eXzheIlwdg>? NVXU[3poRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNlY2OzJpPkK0NVI3PTN{PD;hQi=>
Ca9-22 M{\4dWZ2dmO2aX;uJGF{e2G7 M3[x[|Mh|ryP NFLKOokyKGh? NIr6NpBi[m:uaYPo[ZMhfGinIHHibYxqfHlib3[gTIJTKHSxIHnu[JVk\SCLTD24JJBzd2S3Y4Tpc44> MkjvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMk[1N|IoRjJ2MUK2OVMzRC:jPh?=
HAECs M{DWOmZ2dmO2aX;uJGF{e2G7 MYKxNEDPxE1? MXSxJIg> MmXzxsBifHSnboXheIV{KFSQRj5OtU1{fGmvdXzheIVlKEmFQV2tNUBidmRiVlPBUU0yKGW6cILld5Nqd25? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|NE[1O{c,OjRzM{S2OVc9N2F-
Caco-2 NYrpVm1yTnWwY4Tpc44hSXO|YYm= M1v1TlUxyqEQvF2= NH;Je3Y1QGh? NHH0Z5hFVVOR MoXH[Y5p[W6lZYOgeIhmKG2UTlGgcIV3\Wy|IH;mxsBUS06QMVGsSnh[TDNuwrDMR3QtyqCOT2isxsBJUUZ|QT|CpHpIOTZuwrDQSGU3ScLiYX7kxsBNT0GOU{G2xsBo\W6nc9MgZ48ufHKnYYTl[EB4cXSqIFTlfC=> M4Xi[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MU[xOlk2Lz5{NEG2NVY6PTxxYU6=
MCF-7 MnLYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXixNEDPxE1? NXTIfVBXPDiq NY[zNldjTE2VTx?= NF7oXnhz\X[ncoPld{BDVkZvaX7keYNm\CClZXzsJIN6[2ynIHHydoV{fA>? NHPvZYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG2N|QxPCd-MkSxOlM1ODR:L3G+
SMMC7721 NIj2[GxHfW6ldHnvckBCe3OjeR?= M2XxblI2NzVyIN88US=> MWqyOEBp MYTzeZBxemW|c3XzJJRp\SCneIDy[ZN{cW:wIH;mJJAuSWu2IH;yJJAuTVKNMT:yxsA> MnfZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNkiwOVYoRjJ2MU[4NFU3RC:jPh?=
SGC7901  Mnm1RZBweHSxc3nzJGF{e2G7 NETz[GM2OMLizszN M4XyblI1NzR6L{eyJIg> MUnEUXNQ NFzre2NqdmS3Y3XzJIFxd3C2b4Ppd{Bkd22kaX7l[EB4cXSqIFrBT|Ihe2iUTlG= NFnqcXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG3PFI1OCd-MkSxO|gzPDB:L3G+
7721 MoP2RZBweHSxc3nzJGF{e2G7 MUezNEDPxE1? NUG2WY1QPSCm MVLk[YNz\WG|ZYOgZ4VtdCCycn;sbYZmemG2aX;u NFTCbJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxNVI2Oyd-MkSyNVEzPTN:L3G+
DLD-1  NFfIb3FHfW6ldHnvckBCe3OjeR?= M1v6XFIxyqEQvF2= M2LNO|Q5KGh? NYHhUZh[emWmdXPld{B1cGViQl7JVFMh\XiycnXzd4lwdiCycnWteJJm[XSnZDD3bZRpKDVvYYrhMYRE MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzMUW4NUc,OjR{MUG1PFE9N2F-
HT-29 M2nndWZ2dmO2aX;uJGF{e2G7 M3vsPFIxyqEQvF2= NULpVI1IPDhiaB?= M3PiZ5Jm\HWlZYOgeIhmKEKQSWCzJIV5eHKnc4Ppc44heHKnLYTy[YF1\WRid3n0bEA2NWG8YT3kRy=> NISzeYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxNVU5OSd-MkSyNVE2QDF:L3G+
7402 MnzIRZBweHSxc3nzJGF{e2G7 NFrHT4g{OCEQvF2= M333RlUh\A>? M4KzVoRm[3KnYYPld{Bk\WyuIIDyc4xq\mW{YYTpc44> M1vUSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkGxNlU{Lz5{NEKxNVI2OzxxYU6=
MCF-7 MVLGeY5kfGmxbjDBd5NigQ>? MVqyNEDPxE1? M1Tac|EhcA>? MVjhZo9tcXOqZYOg[ZhxemW|c3nvckBw\iCyaH;zdIhwenmuYYTl[EBGWktiY3:teJJm[XSvZX70JJdqfGhiY3;ubpVo[XSn MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzNkK4PUc,OjR{MU[yPFk9N2F-
MCF-7 NX2xNmRmSXCxcITvd4l{KEG|c3H5 MX6yNEDPxE1? NU\4ZoxjOSCq Mn32bY5kemWjc3XzJINie3Cjc3WtPUBmdnq7bXWgZYN1cX[rdIm= Mn32QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MU[yPFkoRjJ2MkG2Nlg6RC:jPh?=
CRL-2302 MorTSpVv[3Srb36gRZN{[Xl? MX:yNEDPxE1? MkHWNE42KGh? NVnPbpY6cW6qaXLpeJMhSXCnbHnuMYlv\HWlZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJGVzcyCjbnSgRYt1 NEfCb3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyO|kyQCd-MkSyNlc6OTh:L3G+
ARPE-19 NV\DN|M2TnWwY4Tpc44hSXO|YYm= MYmyNEDPxE1? NVj2bWh{OC53IHi= MlfwbY5pcWKrdIOgRZBmdGmwLXnu[JVk\WRicHjvd5Bpd3K7bHH0bY9vKG:oIFXyb{BidmRiQXv0 MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{N{mxPEc,OjR{Mke5NVg9N2F-
MG-63 MVXGeY5kfGmxbjDBd5NigQ>? MkC1NlAh|ryP NX\PVoo5OC53IHi= Mo\CZoxw[2u|IITo[UBEUC2rbnT1Z4VlKHCqb4PwbI9zgWyjdHXkJGVNUzFicILveIVqdiCneIDy[ZN{cW:w NVPVOWRTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyN|k3PDBpPkK0NlM6PjRyPD;hQi=>
SGC-7901  NVXhVlFVSXCxcITvd4l{KEG|c3H5 M4jjTVIxKM7:TR?= NYLreWxEOjRiaB?= NF:wVJpqdmirYnn0d{BEWC2vZXTpZZRm\CCjcH;weI9{cXN? NIrTPXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0NVM2OSd-MkSyOFE{PTF:L3G+
NUGC2 NX[0OVF6TnWwY4Tpc44hSXO|YYm= NHrsbmk2OCEQvF2= MXS0PEBp MlTQSG1UVw>? NWTVelRtcW6lcnXhd4V{KGW6cILld5Nqd25ib3[gTGxCNUFyMjDvdkBJVEFvQUK0JI1wdGWldXzldy=> NHTzbYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
NUGC3 NI[0[mJHfW6ldHnvckBCe3OjeR?= MUC1NEDPxE1? MmLQOFghcA>? M1PGXmROW09? NU\UNnhTcW6lcnXhd4V{KGW6cILld5Nqd25ib3[gTGxCNUFyMjDvdkBJVEFvQUK0JI1wdGWldXzldy=> NX\IPXU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
KATOIII  M{DwcmZ2dmO2aX;uJGF{e2G7 MUO1NEDPxE1? NF:zTZI1QCCq MXLEUXNQ MnLvbY5kemWjc3XzJIV5eHKnc4Ppc44hd2ZiSFzBMWExOiCxcjDIUGEuSTJ2IH3vcIVkfWyncx?= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NECyN{c,OjR{NESwNlM9N2F-
NCI-N87  M3jpTmZ2dmO2aX;uJGF{e2G7 MmLPOVAh|ryP MWS0PEBp M3fzUmROW09? MmXVbY5kemWjc3XzJIV5eHKnc4Ppc44hd2ZiSFzBMWExOiCxcjDIUGEuSTJ2IH3vcIVkfWyncx?= M2Dtc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkS0NFI{Lz5{NEK0OFAzOzxxYU6=
OE19 M1jld2Z2dmO2aX;uJGF{e2G7 MoLoOVAh|ryP NGLSSG01QCCq MWDEUXNQ NVm1[mNLcW6lcnXhd4V{KGW6cILld5Nqd25ib3[gTGxCNUFyMjDvdkBJVEFvQUK0JI1wdGWldXzldy=> NFrkclA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
MKN7 MWHGeY5kfGmxbjDBd5NigQ>? MmnIOVAh|ryP NIqwXJU1QCCq MlvxSG1UVw>? NFrQPW9qdmO{ZXHz[ZMh\XiycnXzd4lwdiCxZjDIUGEuSTB{IH;yJGhNSS2DMkSgcY9t\WO3bHXz NWHLO5ppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
TE4 MnTuSpVv[3Srb36gRZN{[Xl? NG\LXYE2OCEQvF2= NUC1S3VuPDhiaB?= M1\3eWROW09? M1;6SpVxemWpdXzheIV{KHSqZTDlfJBz\XO|aX;uJI9nKEiOQTDjcIF{eyCL MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NECyN{c,OjR{NESwNlM9N2F-
KYSE30 M4fT[2Z2dmO2aX;uJGF{e2G7 MlPBOVAh|ryP MkW2OFghcA>? MVrEUXNQ M{PzNpVxemWpdXzheIV{KHSqZTDlfJBz\XO|aX;uJI9nKEiOQTDjcIF{eyCL NFuw[4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
TE5 M3nERWZ2dmO2aX;uJGF{e2G7 NFzNV4Y2OCEQvF2= NHjieVI1QCCq NILNfmZFVVOR MWL1dJJm\3WuYYTld{B1cGViZYjwdoV{e2mxbjDv[kBJVEFiY3zhd5MhUQ>? NX:3WZpTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFQxOjNpPkK0NlQ1ODJ|PD;hQi=>
TE1 MnjPSpVv[3Srb36gRZN{[Xl? Mn7uOVAh|ryP MlHUOFghcA>? NVXBXnAxTE2VTx?= NFjmd3Z2eHKnZ4XsZZRmeyC2aHWg[ZhxemW|c3nvckBw\iCKTFGgZ4xie3NiSR?= MnfGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NESwNlMoRjJ2MkS0NFI{RC:jPh?=
TE3 NH:3cI1HfW6ldHnvckBCe3OjeR?= M3HjdFUxKM7:TR?= MonoOFghcA>? M1i0Z2ROW09? Mm\KeZBz\We3bHH0[ZMhfGinIHX4dJJme3Orb36gc4YhUEyDIHPsZZN{KEl? NHjQVJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0OFAzOyd-MkSyOFQxOjN:L3G+
KYSE30 NF7kd2RHfW6ldHnvckBCe3OjeR?= MlP6NlAwPTBxMUCwJO69VQ>? MWC0PEBp M{LQ[GROW09? NIDr[ZpqdmirYnn0d{BxNUW{azDhcoQhf2:{dH3hco5qdiCmb4fudoVofWyjdHXkJJAuSWu2IHnuJIEh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ2NECyN{c,OjR{NESwNlM9N2F-
TE1 M{T2fmZ2dmO2aX;uJGF{e2G7 M1TpXFIxNzVyL{GwNEDPxE1? NYXEXmppPDhiaB?= MUXEUXNQ M2XkUolvcGmkaYTzJJAuTXKtIHHu[EB4d3K2bXHucolvKGSxd37y[Yd2dGG2ZXSgdE1Cc3RiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M3f4e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkS0NFI{Lz5{NEK0OFAzOzxxYU6=
TE4 MYjGeY5kfGmxbjDBd5NigQ>? NUTXZ29NOjBxNUCvNVAxKM7:TR?= M4rIPFQ5KGh? MnjOSG1UVw>? MlHpbY5pcWKrdIOgdE1GemtiYX7kJJdwenSvYX7ubY4h\G:5boLl[5Vt[XSnZDDwMWFsfCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MmX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NESwNlMoRjJ2MkS0NFI{RC:jPh?=
HT29 MUPGeY5kfGmxbjDBd5NigQ>? MVSxNEDPxE1? NEmxcmszKGh? MnfDbY5pcWKrdIOgc4YhUkGNMjygSXJMOS9{IHHu[EBUXEGWMzDwbI9{eGixconsZZRqd25? MnLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NkWyPVMoRjJ2Mk[1Nlk{RC:jPh?=
HepG2 MlSzRZBweHSxc3nzJGF{e2G7 NGr5XIEzOCEQvF2= NV;iXng4OjRiaB?= MXXpcohq[mm2czDFVmsyNzJicHjvd5Bpd3K7bHH0bY9vKGGwZDDlcohidmOnczDWRlEucW6mdXPl[EBieG:ydH;zbZM> NVvY[ZJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOFc6ODlpPkK0NlQ4QTB7PD;hQi=>
HepG2 NH31OlhHfW6ldHnvckBCe3OjeR?= M4rLblIxKM7:TR?= MkK5NkBp M1\1coVvcGGwY3XzJHZDOS2rbnT1Z4VlKE[RWF:zZUB1emGwc3PybZB1cW:wYXygZYN1cX[rdIm= MoqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{NEe5NFkoRjJ2MkS3PVA6RC:jPh?=
MC-3 MWTBdI9xfG:|aYOgRZN{[Xl? Mn\4NVAh|ryP NVvTZVJLOjRiaB?= NH7Vc5Nxd3SnboTpZZRm\CCPRWPDMYlv\HWlZXSgZZBweHSxc3nzJIlvKCClZXzsdy=> M1e4fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkewOVI{Lz5{NEK3NFUzOzxxYU6=
Raji  MUPGeY5kfGmxbjDBd5NigQ>? M1HRTFExKM7:TR?= MUKxJIg> M3H2fIJtd2OtczDod2JCTkZiaX7keYNm\CCHcnuxM|IheGixc4Doc5J6dGG2aX;u M{\NelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mk[5OlMxLz5{NEK2PVY{ODxxYU6=
Raji  NUXUVZRzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vHXFExKM7:TR?= NVLJbGF4OSCq NUHiTnlZcW6qaXLpeJMhfGinIHLhd4FtKG:{IHjzRmFHTi2|dHnteYxifGWmIHPlcIwheHKxbHnm[ZJifGmxbjDhcoQhfmmjYnnsbZR6 NYWxT2NqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlk3OzBpPkK0NlY6PjNyPD;hQi=>
A549 MoPnSpVv[3Srb36gRZN{[Xl? MkDaN|Ah|ryP MWKwMlUhcA>? M2PGcGROW09? MoXObY5pcWKrdIOgeIhzd22kaX6tbY5lfWOnZDDDM2VDWM7{IGTodlI{PcLicHjvd5Bpd3K7bHH0bY9v MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ5N{[5Okc,OjR{N{e2PVY9N2F-
HCSMCs MkK4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfxNVAh|ryP NYXxWm1wOjRiaB?= Mn7wZoxw[2u|IF\BRnA1NWmwZIXj[YQhUEODU13DJJBzd2yrZnXyZZRqd25? MmPzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MUKzPFEoRjJ2M{GyN|gyRC:jPh?=
PANC-1 M{DEO2Z2dmO2aX;uJGF{e2G7 M3\6T|IxKM7:TR?= NWDRTIFkPDhiaB?= MXnpcohq[mm2czD0bIUh\XiycnXzd4lwdiCxZjFOmFZFKGmwIILld5BwdnOnIITvJJRp\SCSUFHS{tTDqGGpb37pd5TDqA>? NXrlSnhqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyPVQyOzNpPkK0Nlk1OTN|PD;hQi=>
A549 Ml\CSpVv[3Srb36gRZN{[Xl? MkfUN|Ah|ryP MnXLNE42KGh? MVLEUXNQ MWXpcohq[mm2czD0bIUhfGi{b33ibY4ucW6mdXPl[EBKVC16L1PYR2w5NUy3YzDhZ5Rqfmm2eR?= M3rzV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mke3Olk3Lz5{NEK3O|Y6PjxxYU6=
SW480 MVHGeY5kfGmxbjDBd5NigQ>? MWGxNEDPxE1? MnnCNlAhcA>? NYq0fJRWTE2VTx?= M1zEWZN2eHC{ZYPz[ZMhfGinIFPSWEBi[3Srdnn0fS=> Ml2wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkSzOlYoRjJ2M{K0N|Y3RC:jPh?=
HEK 293 M4m2cmZ2dmO2aX;uJGF{e2G7 MkXvNVAh|ryP Mn;hOUBp NF3RfW5FVVOR MmnDbY5pcWKrdIOgW451NWmwZIXj[YQh|rJvY3H0[Y5qdi:WQ1[0JIFkfGm4aYT5JIFv\CCwdXPs[YFzKM7{LXPheIVvcW5iYXPjeY12dGG2aX;u M1fP[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{K0N|Y3Lz5{NEOyOFM3PjxxYU6=
HEK 293 MnWySpVv[3Srb36gRZN{[Xl? NYfweFFoOTBizszN NFnwTI82KGh? M4e2fmROW09? NFfNelh{fXCycnXzd4V{KHSqZTDDVnQh[WO2aY\peJk> MljVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|MkSzOlYoRjJ2M{K0N|Y3RC:jPh?=
HL-60  MWfGeY5kfGmxbjDBd5NigQ>? NULtcGRSOTBxMkCg{txO MkfJNUBp NVLaWFQzcW6qaXLpeJMhfGinwrDOMkBkcGmwZX7zbZNmgHS{YXP0xsBqdmS3Y3XkJIRq\m[ncnXueIlifGmxbjDpcpRwKGe{YX71cI9kgXSncx?= MonqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|NUewNlAoRjJ2M{W3NFIxRC:jPh?=
HL-60 MnfQSpVv[3Srb36gRZN{[Xl? MUWyJOK2VQ>? NXjLUVJ[OTZiaB?= NGfGNJhFVVOR Mlf4bY5pcWKrdIOgeIhmKGG|c3;jbYF1cW:wIH;mJJBUPjJzIGLh[k0yKGGwZDDOSmFV[zNuIHHu[EB1cGViUlGtbY5lfWOnZDDwbI9{eGixconsZZRqd25ib3[gcpVkdGWjcjDOSmFV[zN? MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN|MEC2PEc,OjR|M{CwOlg9N2F-
HeLa NU\DVHlRTnWwY4Tpc44hSXO|YYm= NVjxSY1KPTBizszN MV:wMlUhcA>? MojDZoxw[2u|IGTSXE0yKG63Y3zlZZIhdWmpcnH0bY9vKGGwZDDUXG5KWCCmb4fuMZJm\3WuYYTpc44> M13SfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{e2PFI4Lz5{NEO3OlgzPzxxYU6=
HUVECs MY\GeY5kfGmxbjDBd5NigQ>? MWOxNOKh|ryP NYDkNFZbOSCq NYHBNZZpcW6qaXLpeJMhfGinIFjEUEBz\WS3Y3XkJGNQYC1{IHX4dJJme3Orb36gZY5lKFCJST2yJJJmdGWjc3W= NWG1OlFXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzPFUyODlpPkK0N|g2OTB7PD;hQi=>
MCF-7 MmfKSpVv[3Srb36gRZN{[Xl? NFX5b5QyOMLizszN NHzBWmUyOC9|MDDtbY4> Mn\odoVlfWOnczD0bIUhXVSSLXTldIVv\GWwdDDFVmsheGixc4Doc5J6dGG2aX;u MoXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR|OUC4NVkoRjJ2M{mwPFE6RC:jPh?=
HGC-27 MX3BdI9xfG:|aYOgRZN{[Xl? Ml:xNUDDvU1? MVOxJIg> M1;JUpN2eHC{ZYPz[ZMhWkGGMECxJJBtfXNiTVutNlIxPi2rbnT1Z4VlKGOnbHygeoli[mmuaYT5JIxwe3N? M1joXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEG2N|Q6Lz5{NESxOlM1QTxxYU6=
PC3  NGHad|JCeG:ydH;zbZMhSXO|YYm= NFK4eYQ2OCEQvF2= NETofmQxNjViaB?= MUHpcohq[mm2czDNTHkuPDR7LXnu[JVk\WRiYYDvdJRwe2m|wrC= Ml7aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2MkS4PFkoRjJ2NEK0PFg6RC:jPh?=
HPAEpiCs  M1rPUWZ2dmO2aX;uJGF{e2G7 NHe1b2E{OCEQvF2= Ml3RNUBp MorlbY5pcWKrdIOgWG5HNc7zIIP0bY12dGG2ZXSgdFQzN3B2NDDNRXBMKHCqb4PwbI9zgWyjdHnvci=> NFrnZXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES0NVg4OCd-MkS0OFE5PzB:L3G+
BeWo NGPaZVhHfW6ldHnvckBCe3OjeR?= NH\nc2QyOMLizszN NYLxZ2w{OiCq NXG3OmJ4cW6qaXLpeJMhTVKNMT:y NHvs[Jk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESzN|g1Pid-MkS0N|M5PDZ:L3G+
A498 MmW1RZBweHSxc3nzJGF{e2G7 NUfzS4NTPTEEoN88US=> MmjXNlQhcA>? NYH6Sm9[eG:2ZX70bYF1\XQEoITo[UBxem9vYYDvdJRwfGmlIHXm[oVkfHNib3[gUmM> NF\sRoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWwPFQ4Pid-MkS1NFg1PzZ:L3G+
NHBE MYrGeY5kfGmxbjDBd5NigQ>? NFfLRWUzNzJyIN88US=> M2LHT|IhcA>? MX3heJRmdnWjdHXzJGlNNTN|IIP0bY12dGG2ZXSgR3hEVDhxSVytPEB{\WO{ZYTpc44> M3ficVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEe5OVI3Lz5{NES3PVUzPjxxYU6=
A375 MWTD[YxtKEmwdnHzbY9vKEG|c3H5 NFfme3oyOOLCk{KwJOK2VQ>? MX2yOEBp NHK1VYJz\WS3Y3XzJI1mdGGwb33hJINmdGxiaX72ZZNqd25? NFLlPIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES2OlA{Pid-MkS0OlYxOzZ:L3G+
HBMEC M33JS2Z2dmO2aX;uJGF{e2G7 NIO1RnEyOCEQvF2= MnvxNUBp NWPvUlNG[myxY3vzJHZGT0ZvaX7keYNm\CCHcHjBNkBmgHC{ZYPzbY9v MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR3OEm4Nkc,OjR2NUi5PFI9N2F-
HCT-15 M4X4XWZ2dmO2aX;uJGF{e2G7 MXGxJIg> MVjheJRmdnWjdHXzJHBITTJvaX7keYNm\CCyaH;zdIhwenmuYYTpc44hd2ZiRYLrxsA> MlXOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{G0NlUoRjJ3NEOxOFI2RC:jPh?=
HCT-15 M2TSRWFxd3C2b4Ppd{BCe3OjeR?= M2HZNVEhcA>? MYPhZo9tcXOqZYOgeIhmKHC{b4TlZ5RqfmViZX\m[YN1eyCxZjDQS2UzyqCjZ3HpcpN1KGO3cnP1cYlvNWmwZIXj[YQh[XCxcITvd4l{ M4nKNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEOxOFI2Lz5{NUSzNVQzPTxxYU6=
786-O Mn3tRZBweHSxc3nzJGF{e2G7 NIGzOWI2OMLizszN MXOyOEBp MlzydI91\W62aXH0[ZPDqHSqZTDwdo8u[XCxcITveIlkKGWoZnXjeJMhd2ZiTlO= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVyOES3Okc,OjR3MEi0O|Y9N2F-
HepG2  NX;5XnpQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\ySmwzOCEQvF2= MXiyOEBp MXPzeZBxemW|c3XzJHRITi4QskGtbY5lfWOnZDDj[YxtKHC{b3zp[oVz[XSrb36gZY5lKGmwdnHzbY9v NHrvS|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW2NFQ5QCd-MkW1OlA1QDh:L3G+
SW480 NYPwVItDTnWwY4Tpc44hSXO|YYm= MoWzNlDDqM7:TR?= NGXkW20yyqCq NY[5TFM2emWmdXPld{B1cGViZYjwdoV{e2mxbjDv[kBCXEZ|IIDyc5RmcW5? NWW2e5JPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OFc5OTZpPkK1OFQ4QDF4PD;hQi=>
MDA-MB-231 M2n3cWZ2dmO2aX;uJGF{e2G7 NIjHRXYzPSEQvF2= MonFNk0{KGh? M4G3R4Rm[3KnYYPld{BxNUWUS{GvNkBidmRiU{GwNGE1KGW6cILld5Nqd25? NW\u[ItKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVU5PzVpPkK1OVU2QDd3PD;hQi=>
HepG2  MmXuSpVv[3Srb36gRZN{[Xl? MlHWNVAh|ryP NYjyZYlbPSCq NVnGR5BM[myxY3vzJJBpd3OyaH;yfYxifGWmIF3BVGt{KGmwZIXj[YQh[nliZYjv[4Vvd3W|IGTHSk3PujF? NX3Dd4FXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OlA1QDhpPkK1OVYxPDh6PD;hQi=>
MCF-7  M2Hu[mZ2dmO2aX;uJGF{e2G7 MkXsNVAh|ryP MmnUNUBp NHr2Z3hqdmirYnn0d{BKVC1zOD3lcohidmOnZDDj[YxtKG2rZ4LheIlwdg>? Ml7WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5MkewNVEoRjJ3N{K3NFEyRC:jPh?=
H1355 NIrU[phHfW6ldHnvckBCe3OjeR?= MoCwNlUh|ryP NUnmdYpCOSCq M3zrZoJtfW62czD0bIUhSlujXWCtbY5lfWOnZDDpcoNz\WG|ZTDpckBxcG:|cHjvMWNpczFiYX7kJJBpd3OyaH:tSXJMKGW6cILld5Nqd25? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd4OUG4NUc,OjV5NkmxPFE9N2F-
PC12 NXnOd3V5TnWwY4Tpc44h[XO|YYm= MYfJcohq[mm2aX;uJI9nKG6nco\lJIdzd3e2aDDmZYN1d3JvbXXkbYF1\WRiTVHQJItqdmG|ZTDhZ5Rqfmm2eTDpckBpfW2jbjDQR|EzKGOnbHzzMEBKSzVyIE2gNkDPxE1w NFKySYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC3O|M3Oyd-MUiwO|c{PjN:L3G+
HT-29 NX64TIRiSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NF\aT3c2KGSjeYO= M2Cye2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGStNlkh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IGfTWFEh[XO|YYmsJGlEPTBiPTC0JO69VS5? NHPsfpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC3PFMyPid-MkWwO|g{OTZ:L3G+
IEC6 M{DlUGZ2dmO2aX;uJIF{e2G7 NXzu[mNpPSCvaX7z MX7Jcohq[mm2aX;uJI9nKE2HS{GvNkBqdiC{YYSgTWVEPiClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gSXJMOS9{IHzvc5AheGixc4Doc5J6dGG2aX;uJIRwe2WmIEOwJI1qdnNiYnXmc5JmKHSxIIP0bY12dGG2aX;uJJdqfGhiMUClJJNmenWvIH\vdkA2KG2rboOgZpkhcW2vdX7vZoxwfHSrbnegcYV1cG:mLDDJR|UxKD1iND6yJO69VS5? NH7QR3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC3PFMyPid-MkWwO|g{OTZ:L3G+
NG 108-15 M2P0XWZ2dmO2aX;uJIF{e2G7 Mlj0R49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDhZo9tcXOqIF3BVGsh[WO2aY\peJkhcW5ibX;1d4UhdmW3cn;icIF{fG:vYTDhcoQhemG2IHfsbY9u[SCqeXLybYQhVkdiMUC4MVE2KGOnbHzzMEBC[3Srdnn0fUA:KDFyIN88UU4> NX34dol1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1N|c{PTRpPkG1OVM4OzV2PD;hQi=>
PC12 NWnBcJdoTnWwY4Tpc44h[XO|YYm= M1:xWlExKHWP NXTLVmpjPSCqcoO= Ml3SRYN1cX[jdHnvckBw\iCQcn[yM2FTTSCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDIU{0yKHC{b4TlbY4hcW6mdXP0bY9vKGG2IEGwJJVOKGGodHXyJFUhcHK|IIDy[ZRz\WG2ZXSge4l1cCCMTlugbY5pcWKrdH;yJHNRPjByMUK1JIZweiBzIHjyJIJm\m:{ZTDjc41xd3WwZDDh[IRqfGmxbjDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MmDnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|NEW2PFUoRjJzM{S1Olg2RC:jPh?=
PC12 MkC1SpVv[3Srb36gZZN{[Xl? NIrZVJkyOCC3TR?= MnXJOUBpenN? MkS4RYN1cX[jdHnvckBw\iCQcn[yM2FTTSCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDIU{0yKHC{b4TlbY4hcW6mdXP0bY9vKGG2IEGwJJVOKGGodHXyJFUhcHK|IIDy[ZRz\WG2ZXSge4l1cCCPRVuyJIlvcGmkaYTvdkBWODF{NjDmc5IhOSCqcjDi[YZwemViY3;tdI92dmRiYXTkbZRqd25iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ NFzs[FE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO0OVY5PSd-MkGzOFU3QDV:L3G+
PC12 NH21UolHfW6ldHnvckBie3OjeR?= M1yweVExKHWP MWW1JIhzew>? MkPERYN1cX[jdHnvckBw\iCQcn[yM2FTTSCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDIU{0yKHC{b4TlbY4hcW6mdXP0bY9vKGG2IEGwJJVOKGGodHXyJFUhcHK|IIDy[ZRz\WG2ZXSge4l1cCCPRVuxJIlvcGmkaYTvdkBRTDl6MEW5JIZweiBzIHjyJIJm\m:{ZTDjc41xd3WwZDDh[IRqfGmxbjDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? NEX1TIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO0OVY5PSd-MkGzOFU3QDV:L3G+
HeLa M4PMSGZ2dmO2aX;uJIF{e2G7 M4T4SVIxKHWP NV3ZR2pvOyCqcoO= MVjJcohq[mm2aX;uJI9nKEWUS{GvNkBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCB{MDD1UUBi\nSncjCzJIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6pIHHuZYx6e2m| MmmxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3N{C2NVUoRjJ|NUewOlE2RC:jPh?=
RD MnfQdWhVWyCjc4PhfS=> MlXwdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKGIHPlcIx{ MoHZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 MWPxTHRUKGG|c3H5 NV3QVXlqeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsdy=> NFLBepA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
RD M2rTXpFJXFNiYYPzZZk> MV\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSSEBk\Wyucx?= NX3E[2FnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC M2Xhd5FJXFNiYYPzZZk> NELqSHRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=> NWLFPWpqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 M4LDc5FJXFNiYYPzZZk> MoD0dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsdy=> NF\x[VM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MDA-MB-231 M2\WZ2Z2dmO2aX;uJIF{e2G7 MorROVAhfU1? MV;Ec5dvemWpdXzheIlwdiCxZjDNUXAzKGmwIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiNUCgeW0h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz M2XTOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUK2NlI3Lz5{MkmyOlIzPjxxYU6=
MDA-MB-231 M3eyWWZ2dmO2aX;uJIF{e2G7 MlvYOVAhfU1? Mm\DSI94dnKnZ4XsZZRqd25ib3[gUW1RQSCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG2IEWwJJVOKGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NIHVNmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmyOlIzPid-MkK5NlYzOjZ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
c-Jun / α-tubulin / p-ERK / ERK / p-AKT / AKT; 

PubMed: 17482134     


ERK regulates c-Jun levels in melanoma. The indicated melanoma cell lines were treated with 50μM PD98059 (PD) or 10μM LY294002 (LY) for 12h in the absence of serum. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-JNK / JNK / Cyclin D1 ; 

PubMed: 17482134     


Inhibition of the MEK/ERK pathway affects c-Jun, P-JNK and cyclin D1 levels. The indicated melanoma cell lines were treated with 50μM PD98059 for 16h. Protein samples were analyzed by Western blots using the indicated antibodies. α-Tubulin reveals equal loading.

p-HER2 / HER2 ; 

PubMed: 24244023     


TE1cells were treated with wortmannin (Akt signal inhibitor), PD98059 (MAPK signal inhibitor), or lapatinib (EGFR and HER2 dual inhibitor) at the indicated doses or with DMSO as a negative control for 48 h. HER2-related signaling molecules were assessed by Western blot. 

MMP9 / XIAP / VEGF ; 

PubMed: 29695559     


SK-Hep1 cells were treated 10 μM PD98059 for 48 h.  Protein levels of MMP-9, XIAP, VEGF, cyclin-D1, and pERK were evaluated with western blotting assay.

17482134 24244023 29695559
体内研究

在大脑缺血前30分钟用PD98059处理小鼠可以明显减轻伤害, 使脑梗塞的体积减小[4]。基于胰腺的湿重和组织学研究发现,提前30分钟用PD98059预处理 (10 mg/kg 静脉注射) 然后每小时注射一次蛙皮素连续三次的小鼠可以明显改善蛙皮素诱导的急性胰腺炎[7]。卡拉胶损伤一小时后用PD98059 (10 mg/kg) 对小鼠进行治疗可以发现与炎症相关的所有指标均有所下降[11]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

体外MEK激酶活性测定:

在含有44-kDa GST-MAPK 或者45-kDa GST-MEK1的体系中检测32P被掺入蛋白 MBP中的情况。 反应体系50 μL ,含有50 mM Tris, pH 7.4/10 mM MgCl2/2 mM EGTA/10 μM [γ-32P]ATP, 加入10 μg GST-MEK1, 0.5 μg GST-MAPK和 40 μg MBP。30°C 孵育15 分钟, 加入Laemmli SDS 样品缓冲液终止反应。磷酸化的MBP 进行10% SDS-PAGE分析
细胞实验:

[1]

- 合并
  • Cell lines: K-Balb, KNRK, v-raf-3Y1, SRA/3Y1, EGFR/3T3, 和K562
  • Concentrations: 溶于DMSO, 终浓度~100 μM
  • Incubation Time: 3 天, 或者7-10天
  • Method:

    细胞按10,000-20,000个/mL的密度接种在多孔平板中进行单层生长。48小时后把不同浓度的PD98059 加到细胞培养基孵育3天。 然后通过胰酶将细胞从平板中消化下来并用Coulter 计数器计数。 将细胞按5,000-10,000每皿的浓度接种在35 mm的培养皿中,培养基中含有0.3% 的琼脂和期望浓度的PD98059,让细胞在软琼脂上生长.经过7-10天的生长, 可以借助解剖显微镜将可见的菌落数出来。


    (Only for Reference)
动物实验:

[7]

- 合并
  • Animal Models: 患有急性胰腺炎的雄性Sprague–Dawley 大鼠
  • Dosages: 10 mg/kg
  • Administration: 静脉注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 14 mg/mL warmed (52.37 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
4% DMSO+30% PEG 300+5% Tween 80+ddH2O
1mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 267.28
化学式

C16H13NO3

CAS号 167869-21-8
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How to formulate this inhibitor for i.p. injection?

  • 回答:

    You can prepare the stock by the vehicle 30% PEG400/0.5% Tween80/5% Propylene glycol, 0.5% CMC.

MEK Signaling Pathway Map

MEK Inhibitors with Unique Features

相关MEK产品

Tags: 购买PD98059 | PD98059供应商 | 采购PD98059 | PD98059价格 | PD98059生产 | 订购PD98059 | PD98059代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID