Fisetin

For research use only. Not for use in humans.

目录号:S2298 中文名称:漆黄素,漆树黄酮,非瑟酮

Fisetin Chemical Structure

CAS No. 528-48-3

Fisetin 是有效的Sirtuin活化化合物(STAC),可以调节Sirtuin

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RMB 1302.21 现货
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客户使用Selleck生产的Fisetin发表文献10篇:

客户使用该产品的1个实验数据:

  • Liver pathology and serum markers of liver injury in mice fed control (Ctrl), or ethanol (EtOH), or ethanol combined with fisetin (E/Fisetin) diet for 4 weeks. (A) Light microscopy with hematoxylin and eosin staining shows lipid accumulation (arrows) in the liver of mice after alcohol exposure, which was alleviated by fisetin administration. (B) Plasma alanine aminotransferase (ALT) and (C) aspartate aminotransferase (AST) activities. Infinity ALT and AST reagents were used formeasuring ALT and AST activities. Scale bar: 50 lm. Results are mean±SD (n = 3 to 6). Results for bars that do not share a letter differed significantly among groups (p < 0.05). Significant differences among groups are determined by analysis of variance followed by Tukey’s test.

    Alcohol Clin Exp Res, 2016, 40(10):2076-2084.. Fisetin purchased from Selleck.

产品安全说明书

Sirtuin抑制剂选择性比较

生物活性

产品描述 Fisetin 是有效的Sirtuin活化化合物(STAC),可以调节Sirtuin
靶点
Sirtuin [1]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MDA-MB-453 NI\rSZpE\WyuII\pZYJqdGm2eTDhd5NigQ>? MnLRNEwhOjBuIESwMEA3OCxiOECsJIFv\CBzMECgxtVO M3njVVI1NCB2ODygZY5lKDd{IHjveZJ{ MUjXbZRpKGmwY4LlZZNqdmdiY3;uZ4VvfHKjdHnvcpMhd2ZiZnnz[ZRqdiBqMDygNlAtKDRyLDC2NEwhQDBuIHHu[EAyODBiwsXNLUwhfGincnWge4F{KGFiZHXjdoVie2Vib3Lz[ZJ3\WRiaX6gZ4VtdCCwdX3i[ZIhcW5iZHnm[oVz\W62IITpcYUhcW62ZYL2ZYx{KCh{NDygOFgtKGGwZDC3NkBpd3W{czmu M3j3W|MxPDNzNkmy
SGC7901 NWnCZWdUWHKxbHnm[ZJifGmxbjDhd5NigQ>? MYCwMEAyNCB3LDCxNEwhOTVib4KgNlAhyrWP NEXqNZg1QCCq Mn\lbY5pcWKrdIOgdJJwdGmoZYLheIlwdg>? MmDpNlk5ODV3OEC=
A549 M3XPcmNmdGxidnnhZoltcXS7IHHzd4F6 MX[wMEAyOCxiMkCsJFMxKG:{IESwJOK2VQ>? M4TrTFI1NCB2ODDvdkA4OiCq NXrseWhI\mm|ZYTpckB4[XNiZX\m[YN1cX[nIHnuJIlvcGmkaYTpcochfGinIHfyc5d1cCCxZjDBOVQ6KGOnbHzzJIlvKGFiZH;z[U0h[W6mIITpcYUu\GWyZX7k[Y51KG2jbn7ldi=> MWOyPVQ3Pzh3OR?=
ARPE-19 NX\ycGg6S2WubDDtbYdz[XSrb36gZZN{[Xl? NYHBcWFJPSCxcjCxNEDPxE1? MVuyOEBp NH;NR2hxemW2cnXheI1mdnRid3n0bEBncXOndHnuJI1iemunZHz5JJJm\HWlZXSgZ4VtdCCvaXfyZZRqd25iaX6gSWdHNXS{ZXH0[YQhSVKSRT2xPUBk\Wyucx?= M3nGW|I6Ojl4MEew
SCC-23 M4nHWWNmdGxidnnhZoltcXS7IHHzd4F6 M{H0[|AuOTByIN88US=> NF;IdWszPCCq M{jXPIZqe2W2aX6gd4lodmmoaXPhcpRtgSCrbnjpZol1\WRiY3XscEB3cWGkaXzpeJkhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZIhMHOrZ37p[olk[W62bImge4hmdiC2aHWgZ49v[2WwdILheIlwdiCrczDobYdp\XJidHjhckAzOCEQvF2p MlSyNlk{OTJ3MkC=
Tca-8113 MWrD[YxtKH[rYXLpcIl1gSCjc4PhfS=> M{LMRlAuOTByIN88US=> NXHUXnFrOjRiaB?= NUXTXmlr\mm|ZYTpckB{cWewaX\pZ4FvfGy7IHnubIljcXSnZDDj[YxtKH[rYXLpcIl1gSCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meiBqc3nncolncWOjboTsfUB4cGWwIITo[UBkd26lZX70doF1cW:wIHnzJIhq\2incjD0bIFvKDJyIN88UUk> NFS3THUzQTNzMkWyNC=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-IKKα/β / IKKβ / IKKα / p-IκBα / IκBα / p-p65 / p65 ; 

PubMed: 23861901     


Similar experiments were conducted with increasing doses of fisetin (1 to 10 µM), for a 15 minutes incubation with RANKL. Blots are representative of 3 independent experiments.

XBP1s / ATF6 / CHOP / ATF4 / PERK; 

PubMed: 25016296     


A375 and 451Lu cells were treated with varying doses of fisetin for 24 h and western blot analysis was performed. Equal loading was confirmed by reprobing for β-actin. Data shown are representative of three independent experiments.

DR3 / pNF-κB / NF-κB ; 

PubMed: 19670328     


Representative immunoblots showing expression of DR3, pNF-κB/p65 and NF-κB/p65 in cells treated with fisetin (0-40 μM).

p-mTOR / mTOR / Raptor / Rictor ; 

PubMed: 20530556     


Effect of fisetin on mTOR, Raptor and Rictor shown by western blot.

23861901 25016296 19670328 20530556
Immunofluorescence
p-4EBP1 / p-eIF4B / p-rpS6 ; 

PubMed: 20530556     


Effect of fisetin on mTOR target proteins. Immunofluorescent imaging of mTOR target proteins upon fisetin treatment is shown. Cells were seeded and cultured for 24 h in two-chamber glass slides followed by treatment with indicated doses of fisetin for 48 h before fixing and labeling with antibodies. For labeling, anti-rabbit antibodies against (A) p-4EBP1 (S65), (B) p-eIF4B (S422) and (C) p-rpS6 (S235/S236) (all from Cell Signaling Technology) were used. Alexa Fluor goat and anti-rabbit IgGs (Invitrogen) were used as secondary antibodies. 4′,6-Diamidino-2-phenylindole (DAPI) was used to counter stain the nucleus; ×600.

uPA ; 

PubMed: 23940799     


Cells were fixed, permeabilized, and immunostained with anti-uPA antibody (red), and cell nuclei were counter-stained with DAPI reagent. 

20530556 23940799
Growth inhibition assay
Cell viability; 

PubMed: 19670328     


Cell growth and viability of human PaC AsPC-1cells treated with fisetin (0-80 μM for 24 and 48 h) as measured by MTT assay. Each concentration of fisetin was repeated in ten wells. The values are represented as percent viable cells where vehicle (DMSO)-treated cells were regarded as 100% viable. Data is represented as mean ± SE of three independent experiments. Inset: Structure of fisetin.

19670328

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

溶解度 (25°C)

体外 DMSO 57 mg/mL (199.13 mM)
Ethanol 3 mg/mL (10.48 mM)
Water Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 286.24
化学式

C15H10O6

CAS号 528-48-3
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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操作手册

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Sirtuin Signaling Pathway Map

相关Sirtuin产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID