Tamoxifen (ICI 46474) Citrate

For research use only. Not for use in humans.

目录号:S1972 中文名称:枸橼酸他莫昔芬

Tamoxifen (ICI 46474) Citrate Chemical Structure

CAS No. 54965-24-1

Tamoxifen Citrate (ICI 46474) 是一种雌激素受体 estrogen receptor 调节剂 (SERM),通过竞争性抑制雌激素结合发挥作用。Tamoxifen Citrate 也是一种有效的 Hsp90 激活剂,可增强Hsp90分子伴侣ATPase的活性。Tamoxifen 可诱导凋亡与自噬。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1061.43 现货
RMB 783.61 现货
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客户使用Selleck生产的Tamoxifen (ICI 46474) Citrate发表文献14篇:

客户使用该产品的1个实验数据:

  • Effects of DPN, LY500307, Raloxifene and Tamoxifen on cell viability in BSO-treated FRDA fibroblasts. BSO concentration was 1 mM and all steroid concentrations were 100 nM. Depicted are mean ± SD for n= 8 per group. * indicated p<0.05 versus BSO alone-treated cells.

    University of North Texas Health Science Center, 2014.. Tamoxifen (ICI 46474) Citrate purchased from Selleck.

产品安全说明书

Estrogen/progestogen Receptor抑制剂选择性比较

生物活性

产品描述 Tamoxifen Citrate (ICI 46474) 是一种雌激素受体 estrogen receptor 调节剂 (SERM),通过竞争性抑制雌激素结合发挥作用。Tamoxifen Citrate 也是一种有效的 Hsp90 激活剂,可增强Hsp90分子伴侣ATPase的活性。Tamoxifen 可诱导凋亡与自噬。
靶点
ER [1]
(Cell-free assay)
体外研究

Tamoxifen具有抗雌激素活性(ER)而表现出抗肿瘤活性。在不同ER来源(种属),蛋白浓度和测定条件下,Tamoxifen对ER的表观亲和力在30到0.01%之间。Tamoxifen与ER的结合进一步引起抑制雌激素相关基因表达的抑制,包括被肿瘤通过自分泌或者旁分泌机制分泌的生长因子和血管生长因子。Tamoxifen也直接诱导细胞程序性死亡。[1] Tamoxifen抑制MCF-7细胞 [3H]胸腺嘧啶插入和DNA聚合酶活性,同时引起细胞DNA含量减少和细胞数目减少。Tamoxifen对MCF-7细胞生长的抑制作用可以通过在培养基中加入雌二醇逆转。2 和6 μM Tamoxifen降低S期细胞比例,增加G1期细胞比例。10 μM的Tamoxifen导致细胞在48小时内死亡。[2]Tamoxifen治疗10天后,抑制MCF-7的生长,IC50值为~10 nM。Tamoxifen抑制MCF-7的纤维蛋白溶解酶原激活性,并抑制雌二醇激活的纤维蛋白溶解酶原激活性。Tamoxifen也会引起细胞内孕酮受体水平小幅增加。[3]Tamoxifen抑制前列腺癌细胞系PC3, PC3-M和 DU145,IC50从5.5到10 μM,这与蛋白激酶C的抑制和p21的诱导相关。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MCF7 cells NVHzVng4S3m2b4TvfIlkyqCjc4PhfS=> NEKzXWwyQCCq NFXjOIhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOS0Z5IHPlcIx{KGGodHXyJFE5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVAvOiEQvF2= NHvue2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUmzNlAzPCd-MUm5N|IxOjR:L3G+
human MDA-MB-231 cells MYjDfZRwfG:6aXRCpIF{e2G7 MVSxPEBp NFP3OHlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEG4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUKuN{DPxE1? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl|MkCyOEc,OTl7M{KwNlQ9N2F-
human Ishikawa cells NIjnRnJEgXSxdH;4bYPDqGG|c3H5 NIq2e4QyQCCq M3zPOGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGl{cGmtYYfhJINmdGy|IHHmeIVzKDF6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTJwNTFOwG0> MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl|MkCyOEc,OTl7M{KwNlQ9N2F-
human DU145 cells NUi0Ro84S3m2b4TvfIlkyqCjc4PhfS=> NFzVRVEyQCCq MWfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEWVE1PSClZXzsd{Bi\nSncjCxPEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUG1MlI2KM7:TR?= MlHiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7M{KwNlQoRjF7OUOyNFI1RC:jPh?=
MCF-7 NVXvOWRvTnWwY4Tpc44h[XO|YYm= NIr3VIZKdmirYnn0bY9vKG:oIGuzTH11cHmvaXTpcoUhcW6lb4Lwc5JifGmxbjDpcpRwKE2FRj23JINmdGy|LDDJR|UxRTdwMt88US=> Mn;hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5M{[zNEc,OTV5M{[zNFww[T5?
MCF-7 MYTGeY5kfGmxbjDhd5NigQ>? M3SwU2Vn\mWldHn2[UBld3OnIH\vdkBcO0ifLTDld5Rz[WSrb3ygZYdicW6|dDDwdo9tcW[ncnH0bY9vKG:oIF3DSk04KGOnbHzzMEBGSzVyPUCuNFAxOTIQvF2= NELRSIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[5OlgyLz5{N{[5OlgyRC:jPh?=
BT-20 NV\K[VJ5TnWwY4Tpc44h[XO|YYm= M4nO[|ExKHWP NHT0NZBKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QheHKxbHnm[ZJifGmxbjDv[kBDXC1{MDDj[YxteyCrbjDwdoV{\W6lZTDv[kBVWEViYYSgNVBmNTViTTDjc45k\W62cnH0bY9vNCCLQ{OwQVUvP87:TR?= MmXiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4OU[4NUc,Ojd4OU[4NVww[T5?
MCF-7 Mli1SpVv[3Srb36gZZN{[Xl? M2DWU|EhfU1? NFXjSpdKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QheHKxbHnm[ZJifGmxbjDv[kBOS0ZvNzDj[YxteyCrbjDwdoV{\W6lZTDv[kAyOGVvNjDNJINwdmOnboTyZZRqd25ib3[gX|NJZS1iZYP0doFlcW:uIDjFNkktKEmFM{C9Ok42|ryP Mn3rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4OU[4NUc,Ojd4OU[4NVww[T5?
OHS-50 NXP2fndyeUiWUzDhd5NigQ>? MXvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| NH\uUlA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi96MkCxOVg4Lz56MkCxOVg4RC:jPh?=
MCF-7 MoXxR5l1d3O2YYTpZ4l1gSCjc4PhfS=> Mn\DNUB2VQ>? MnrvR5l1d3O2YYTpZ4l1gSCjZ3HpcpN1KE2FRj23JINmdGy|IHnuJJRp\SCycnXz[Y5k\SCxZjCxJJVOd2yjcjDFNkAp\XO2cnHkbY9tMSxiSVO1NF05|ryP MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86ODh7M{OyK|46ODh7M{OyQE9iRg>?
MCF-7 NV3WUXlTS3m2b4TvfIlkcXS7IHHzd4F6 NUPy[XRzOSC3TR?= MlHyR5l1d3SxeHnjbZR6KGGpYXnud5QhVUOILUegZ4VtdHNiaX6geIhmKGGkc3XuZ4Uhd2ZiMTD1UUBGOiBqZYP0doFlcW:uKTygR5l1d3SxeHnjbZR6RTlwON88US=> NX3B[HM1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUC4PVM{Oid-OUC4PVM{OjxxYU6=
MCF-7 MlvsR5l1d3SxeHnjbZR6KGG|c3H5 M1\hWlEhfU1? NGrqZnFEgXSxdH;4bYNqfHliYXfhbY5{fCCPQ1[tO{Bk\WyuczDpckBxemW|ZX7j[UBw\iBzIIXNJGUzKCinc4TyZYRqd2xrLDDDfZRwfG:6aXPpeJk:OTQQvF2= M{L1cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzlyOEmzN|IoRjlyOEmzN|I9N2F-
MCF-7 NGXWTpZHfW6ldHnvckBie3OjeR?= NG[yTYFKdmirYnn0bY9vKG:oIHXzeJJw\2WwLXnu[JVk\WRicILvcIln\XKjdHnvckBqdiCqdX3hckBOS0ZvNzDidoVie3RiY3HuZ4VzKGOnbHzzMEBKSzVyPUCuOFgy|ryP NGTsSXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97MUW0PVY{Lz57MUW0PVY{RC:jPh?=
Ishikawa cells MnK2RY51cWW|dILv[4VvcWNiYYPzZZk> M{nQN|Qh\GG7cx?= NH:3e29CdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDJd4hqc2G5YTDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGW|dILv[4VvNWmwZIXj[YQh[WytYXzpcoUheGixc4DoZZRie2ViYXP0bZZqfHliYX\0[ZIhPCCmYYnzJIJ6KEWOSWPBMEBKSzVyPUCuOFLPxE1? NUTnWIt5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUe4OFE3Oyd-OUe4OFE3OzxxYU6=
NIH-3T3-G185 NH\3XmVHfW6ldHnvckBie3OjeR?= M1P0WHRRZ1SUQV7TVG9TXEWUOjDpcohq[mm2aX;uJI9nKESjdX7vdpVjcWOrbjDl[oZtfXhiaX6gUmlJNTOWMz3HNVg2KGOnbHzzMEBKSzVyPU[uOO69VQ>? NIrQV2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUexOlUyPCd-MUG3NVY2OTR:L3G+
NIH-3T3-G185 M3zMNGZ2dmO2aX;uJIF{e2G7 MVHUVH9VWkGQU2DPVnRGWjpiaX7obYJqfGmxbjDv[kBNTFNvN{WxJIVn\my3eDDpckBPUUhvM2SzMWcyQDViY3XscJMtKEmFNUC9NVIvOc7:TR?= NFPzemc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUexOlUyPCd-MUG3NVY2OTR:L3G+
NIH-3T3-G185 M1v5TWZ2dmO2aX;uJIF{e2G7 NFyye3NVWF:WUlHOV3BQWlSHUkqgbY5pcWKrdHnvckBw\iCUaH;kZY1qdmViMUKzJIVn\my3eDDpckBPUUhvM2SzMWcyQDViY3XscJMtKEmFNUC9N|EvPM7:TR?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTdzNkWxOEc,OTF5MU[1NVQ9N2F-
MCF-7-2a Ml;WRY51cWW|dILv[4VvcWNiYYPzZZk> NYTVbGROSW62aXXzeJJw\2WwaXOgZYN1cX[rdImgbY4hVUOILUetNoEh[2WubIOgZZMh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{Bz\WS3Y3Wg[ZN1emGmaX;sJIVn\mWldDDifUA2OCVuIFnDOVA:OC53zszN NVHL[mxvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK0N|ExPjNpPkGyOFMyODZ|PD;hQi=>
MCF-7-2a NWfDOpUzTnWwY4Tpc44h[XO|YYm= MUfJcohq[mm2aX;uJI9nKGW|dILh[IlwdCCrbnT1Z4VlKGW|dILv[4VvKHKnY3XweI9zKHS{YX7zZ5JqeHSrb37hcEBi[3SrdnH0bY9vKGmwIF3DSk04NTKjIHPlcIx{NCCLQ{WwQVAvPc7:TR?= M4DOclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NkeyNlQ6Lz5zMk[3NlI1QTxxYU6=
SK-E2 MnixSpVv[3Srb36gZZN{[Xl? M1rHUXRRZ1SUQV7TVG9TXEWUOjDpcoNz\WG|ZTDpckBjd2SrcImgbY51emGlZXzseYxieiCjY3P1cZVt[XSrb36gLGJw\GmyeUqgNE4zKHWPKTDpckBUUy2HMjDj[YxteyBqZYjwdoV{e2mwZzDCV2VRMSxiSVO1NF0yPS52zszN MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjd|OUe1PUc,OTJ5M{m3OVk9N2F-
SK-E2 MkTLSpVv[3Srb36gZZN{[Xl? MXHUVH9VWkGQU2DPVnRGWjpiaX7jdoVie2ViaX6g[IlpgWS{b3\seY9z\XOlZXnuJIlvfHKjY3XscJVt[XJiYXPjeY12dGG2aX;uJEhlcWi7ZILv[ox2d3Knc3PlbY47KDFidV2pJIlvKFONLVWyJINmdGy|IDjlfJBz\XO|aX7nJGJUTVBrLDDJR|UxRTJ|LkROwG0> NXS0dWpORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK3N|k4PTlpPkGyO|M6PzV7PD;hQi=>
DAOY NYT6SnNneUiWUzDhd5NigQ>? NFnFe4RyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?= NIXvR|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkiyOVk{PSd-MUK4NlU6OzV:L3G+
MCF-7 NW\acYNXTnWwY4Tpc44h[XO|YYm= NV[5U2FuUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgUWNHNTdiYoLlZZN1KHS3bX;yJINmdGy|IIXzbY5oKE2FRj23JIF{e2G7LDDJR|UxRTBwNUlOwG0> MkTuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ6MkW5N|UoRjF{OEK1PVM2RC:jPh?=
Caco-2 MUfGeY5kfGmxbjDhd5NigQ>? MYXUVH9VWkGQU2DPVnRGWjpidILhcpNmeGm2aHXsbYFtKHS{YX7zdI9zfCCxZjDSbI9l[W2rbnWgNVI{KCikYYPhcEB1dyCjcHnjZYwqKGmwIFPhZ48uOiClZXzsd{whUUN3ME2yPe69VQ>? NFHkd2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUO4OlQ5Oid-MUWzPFY1QDJ:L3G+
HeLa MWLGeY5kfGmxbjDhd5NigQ>? MVnJcohq[mm2aX;uJI9nKDF5LXLleIEu\XO2cnHkbY9tKG2nZHnheIVlKGy3Y3nm[ZJie2VidILhcpNkemmydHnvckBqdiCKZVzhJINmdGy|IHX4dJJme3OrbnegbJVu[W5iZYP0do9o\W5icnXj[ZB1d3JiYXzwbIE8KEWURTDhd5NigSxiSVO1NF0xNjZ{Mt88US=> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZ3OEi1NUc,OTV4NUi4OVE9N2F-
MCF-7 NWG4O2tuSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NEjiSFFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBqdiCPQ1[tO{BpfW2jbjDidoVie3RiY3HuZ4VzKGOnbHzzMEBKSzVyPUKuN:69VQ>? M2rXXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NkW4PFc2Lz5zNU[1PFg4PTxxYU6=
MDA-MB 231 MXrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MoPnRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgbY4hVUSDLV3CJFI{OSCqdX3hckBjemWjc4SgZ4Fv[2W{IHPlcIx{NCCLQ{WwQVExNjcQvF2= MlzSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV4NUi4O|UoRjF3NkW4PFc2RC:jPh?=
MCF7 NHfWdG5HfW6ldHnvckBie3OjeR?= M4DaUF4yOCC3TR?= NYPnVZpnSWexbnnzeEBi[3Srdnn0fUBifCCHUnHsdIhiKGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhTTJvaX7keYNm\CCudXPp[oVz[XOnIHflcoUhfHKjboPhZ5RqfmG2aX;uJIlvKE2FRkegZ4VtdHNiYYSgQlExKHWP NIHHXGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkezOVEzOCd-MU[3N|UyOjB:L3G+
MCF7 MYHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MVOzJI1oN2up MnvsOFghcHK| NF;wWWFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JG1ETjdiY3XscJMh[XRiMzDt[{9s\y:mYYmgZYZ1\XJiNEigbJJ{NCCLQ{WwQVUvODoQvF2= NFTYSJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkm3PVM{Pyd-MU[5O|k{Ozd:L3G+
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MCF7 MnfqR5l1d3SxeHnjbZR6KGG|c3H5 NW[2eIJjS3m2b4TvfIlkcXS7IHHjeIl3cXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCkeTDNWHMh[XO|YYmsJGlEPTB;M{JOwG0> MnzpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4OECwOlQoRjJzNkiwNFY1RC:jPh?=
ER-positive MCF7 MXfBcpRq\XO2cn;n[Y5q[yCjc4PhfS=> NYHi[Vd2OjBibl2= MknoOFghcHK| NFflfm9CdnSrZYP0do9o\W6rYzDhZ5Rqfmm2eTDpckBpfW2jbjDFVk1xd3OrdHn2[UBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhOTekZYThJIV{fHKjZHnvcE1qdmS3Y3XkJJNm[3KndHXkJIFtc2GuaX7lJJBpd3OyaHH0ZZNmKGGldHn2bZR6KGG2IEKwJI5OKGGodHXyJFQ5KGi{czDifUBxcG:|cHjhMYxq\2i2IILldI9zfGW{IHPo[Y1qdHWvaX7ld4NmdmOnIHHzd4F6 NUDOXIxORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4NFA5PTlpPkKxPFAxQDV7PD;hQi=>
HEK293 MmrjRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MU[0PEBpenN? NFvySolCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjFT|I6OyClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGw{txO MkHLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
MCF7 MkLwRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MnTwOFghcHK| MUPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2FRkegZ4VtdHNiZYjwdoV{e2mwZzDld5Rzd2enbjDy[YNmeHSxcjDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTF{LkS4{txO MlvOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
MDA-MB-231 NFXZOYlCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NXTiRW1RPDhiaILz NFrXdVNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIV{fHKxZ3XuJJJm[2WydH;yMYRm\mmlaXXueEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHX4dJJme3Orbneg[ZN1em:pZX6gdoVk\XC2b4KgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zOD65{txO NX\BcZV7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4O|E5OTJpPkKxPFcyQDF{PD;hQi=>
Ishikawa M2LWUGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NE\0fHM1QCCqcoO= MkfBRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCLc3jpb4F4[SClZXzsd{BmgHC{ZYPzbY5oKGW|dILv[4VvKHKnY3XweI9zKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlIvPc7:TR?= Ml7uQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6N{G4NVIoRjJzOEexPFEzRC:jPh?=
A673 M3vPcZFJXFNiYYPzZZk> M12yR5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy| Mnq5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2N{KwOFUoRjJ{NEeyNFQ2RC:jPh?=
HEK293 MnrmSpVv[3Srb36gZZN{[Xl? MnXwNU42KGi{cx?= NIrtU|FFcXOybHHj[Y1mdnRib3[gX|NJZUyVRDDmdo9uKGi3bXHuJFVJXDZicnXj[ZB1d3JiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjZoTldkAyNjViaILzJIJ6KGyrcYXp[EB{[2mwdHnscIF1cW:wIHPveY51cW6pLDDLbV0yNjB2Md88US=> MknSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3M{exOVMoRjJ{NUO3NVU{RC:jPh?=
AH109 M3zQWmZ2dmO2aX;uJIF{e2G7 M1XPSlI1KGi{cx?= NEDTSI9CdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFXSZoV1[SC{ZXPldJRweiCOQlSg[ZhxemW|c3XkJIlvKHmnYYP0JGFJOTB7IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhcW62ZYLhZ5Rqd25id3n0bEBUWkNzIHHmeIVzKDJ2IHjyd{BjgSCjbIDoZU1o[WyjY4Tvd4ll[XOnIHHzd4F6NCCLQ{WwQVEvPjcQvF2= MlHLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEeyNVcoRjJ{NkS3NlE4RC:jPh?=
AH109 MXjGeY5kfGmxbjDhd5NigQ>? M3\3UFI1KGi{cx?= NUHreItCSW62YXfvcol{fCCjY4Tpeol1gSCjdDDFVoFteGijIILlZ4VxfG:{IFzCSEBmgHC{ZYPz[YQhcW5ieXXhd5QhSUhzMEmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCrboTldoFkfGmxbjD3bZRpKFOUQ{GgZYZ1\XJiMkSgbJJ{KGK7IHHsdIhiNWejbHHjeI9{cWSjc3WgZZN{[XluIFnDOVA:Oi53NN88US=> NXH1Opd[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
MCF7 NEnufG1CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NHrzVHgzPCCqcoO= M1PhT2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIHPlcIwhfmmjYnnsbZR6KGGodHXyJFI1KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NVkvPTUQvF2= NXzHUZpYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2OFczOTdpPkKyOlQ4OjF5PD;hQi=>
DLD1 M13ndGN6fG:2b4jpZ4l1gSCjc4PhfS=> MXW5OkBpenN? MYLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDEUGQyKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;ND63PO69VQ>? NEDZcW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
MCF7 MULDfZRwfG:6aXPpeJkh[XO|YYm= NUnHOIpOQTZiaILz NWjHTHF1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjZoTldkA6PiCqcoOgZpkhW1KEIHHzd4F6NCCLQ{WwQVcvOc7:TR?= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
NIH/3T3 NYrW[mliS3m2b4TvfIlkcXS7IHHzd4F6 NFS1RXM6PiCqcoO= M4CxfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KG2xdYPlJG5KUC9|VEOgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF04NjJ4zszN MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
518A2 NWL3OZdlS3m2b4TvfIlkcXS7IHHzd4F6 MkfTPVYhcHK| M1LEWmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFUyQEF{IHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUC9O{43Os7:TR?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
A2780 NEHFVlVEgXSxdH;4bYNqfHliYYPzZZk> M1HuUFk3KGi{cx?= M37jdmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGEzPzhyIHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUC9O{44P87:TR?= NELWWXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke0PVM6Oid-MkK3OFk{QTJ:L3G+
Lipo MkfER5l1d3SxeHnjbZR6KGG|c3H5 NVvJXIN2QTZiaILz NIr3SYVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNcXCxIHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUC9PE43PM7:TR?= NW\TWlFwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO|Q6Ozl{PD;hQi=>
A253 M{\OWWN6fG:2b4jpZ4l1gSCjc4PhfS=> NHjVXXI6PiCqcoO= MUfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlU{KGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;OD65Nu69VQ>? M{Du[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{S5N|kzLz5{Mke0PVM6OjxxYU6=
A549 NHTjbnhEgXSxdH;4bYNqfHliYYPzZZk> MVW5OkBpenN? MknlR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{Bi\nSncjC5OkBpenNiYomgV3JDKGG|c3H5MEBKSzVyPUmuOlbPxE1? NYft[HUyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3OFk{QTJpPkKyO|Q6Ozl{PD;hQi=>
850C MlfsR5l1d3SxeHnjbZR6KGG|c3H5 M{XQWVk3KGi{cx?= NH7Lb2lEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckA5PTCFIHPlcIx{KGGodHXyJFk3KGi{czDifUBUWkJiYYPzZZktKEmFNUC9NVEvODoQvF2= MmLZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5NEmzPVIoRjJ{N{S5N|kzRC:jPh?=
KIF5 MVLUc5hq[2m2eTDhd5NigQ>? NFfQdIwzPCCqcoO= MoW2WI95cWOrdImgZYdicW6|dDDoeY1idiCNSV[1JINmdGy|IHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KEOnbHzUbZRmeiCEbIXlJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVI{Njd3zszN NX3heGp4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK3O|U1PzRpPkKyO|c2PDd2PD;hQi=>
MCF7 MXXDfZRwfG:6aXPpeJkh[XO|YYm= NIPDWng4OiCqcoO= MojqR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC3NkBpenNiYomgW3NVOSCjc4PhfUBqdiCycnXz[Y5k\SCxZjCyNEUhTkKVLDDJR|UxRThwNt88US=> MlLRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7MEG4PVUoRjJ{OUCxPFk2RC:jPh?=
HL60 Mkn1R5l1d3SxeHnjbZR6KGG|c3H5 NH\VcmU4OiCqcoO= NH;2SpVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJVDZyIHPlcIx{KGGodHXyJFczKGi{czDifUBYW1RzIHHzd4F6KGmwIIDy[ZNmdmOnIH;mJFIxLSCIQmOsJGlEPTB;MUSuN:69VQ>? MnyxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7MEG4PVUoRjJ{OUCxPFk2RC:jPh?=
C41 DE3 NY\0TZlkTnWwY4Tpc44h[XO|YYm= NUm1e4N4PjBidV2= MU[xNEBucW6| NXLsbI11UW6jY4TpeoF1cW:wIH;mJGN[WDKENjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBGe2OqZYLpZ4hq[SClb3zpJGM1OSCGRUOgZ4VtdHNiYYPz[ZN{\WRiYYOgcY9lcW[rZXSgZZBwNVB2NUCgNmI3KGGmZIXjeEBnd3KvYYTpc44h[XRiNkCgeW0h[W[2ZYKgNVAhdWmwczDifUBGW0lvTFOtUXMh[W6jbInzbZMhcW5icILld4Vv[2Vib3[gUmFFWEh? M3r4[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUSyN|E4Lz5{Mkm0NlMyPzxxYU6=
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C41 DE3 MnW2SpVv[3Srb36gZZN{[Xl? NFHkbWsyODBidV2= MWGzNEBucW6| MnX5TY5i[3SrdnH0bY9vKG:oIFPZVFJDPiBqdX7rco94diCxcnnnbY4qKGW6cILld5Nm\CCrbjDFd4Np\XKrY3jpZUBkd2yrIFO0NUBFTTNiY3XscJMh[XO|ZYPz[YQh[XNidIL5dJRq[yCyZYD0bYRmKDF6OF\IXXFFWUVzOUSge4l1cCC|aYTlJI9nKGGmZIXjeEBnd3KvYYTpc44hdG:lYXzpfoVlKHSxIFfsclE6OyCjdDCxNFAhfU1iYX\0[ZIhOzBibXnud{BjgSCOQz3NV{9OWyCjbnHsfZNqeyCrbjDwdoV{\W6lZTDv[kBPSUSSSB?= M4TGXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OUSyN|E4Lz5{Mkm0NlMyPzxxYU6=
MCF7 NYPyOVBmS3m2b4TvfIlkcXS7IHHzd4F6 M{n4O|Q5KGi{cx?= M2Tmb2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNU41PM7:TR?= MnrLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMkKyPFEoRjJ|MEKyNlgyRC:jPh?=
MDA-MB-231 NGruO45EgXSxdH;4bYNqfHliYYPzZZk> Ml3aOFghcHK| MVzDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTJwNEJOwG0> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB{MkK4NUc,OjNyMkKyPFE9N2F-
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Ishikawa cells NFjLVYNCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MWC+OFAhfU1? NYH5TGVEPDhiaILz MXzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEm|aHnrZZdiKGOnbHzzJIF1KD52MDD1UUBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5 NUHLUXQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0PFI6PDRpPkK5OFgzQTR2PD;hQi=>
MCF7 M{XmW2Z2dmO2aX;uJIF{e2G7 NYnscmd5OSC3TR?= NEDLZY5CdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFXSZYxxcGFiaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iZYP0doFlcW:uLXnu[JVk\WRiUHfSJI1TVkFiZYjwdoV{e2mxbjDheEAyKHWPIHL5JHN[SlJiR4Ll[Y4h\HmnIHLhd4VlKFKWLWDDVkBidmGueYPpdy=> MofVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OEK5OFQoRjJ7NEiyPVQ1RC:jPh?=
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insect cells NGntTZNHfW6ldHnvckBie3OjeR?= NFnHcWwzKGi{cx?= MV7EbZNxdGGlZX3lcpQhd2ZiRnz1c5Jud26nIFXTNkBIemWnbjDmdo9uKG[3bHygcIVv\3SqIHj1cYFvKEWULXHsdIhiKGW6cILld5Nm\CCrbjDpcpNm[3RiY3XscJMh[W[2ZYKgNkBpenNiYomg[ox2d3Knc3PlcoNmKHCxbHHybZpifGmxbjDhd5NigSxiSVO1NF0yNjR{zszN MkD2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEeyNlEoRjJ7NUi3NlIyRC:jPh?=
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Lucena 1 NUDGbWxVTnWwY4Tpc44h[XO|YYm= NGrNb5AyOi53IIXN NGfSR2oyKGi{ M{K1NWlvcGmkaYTpc44hd2ZiUD3ndE1u\WSrYYTl[EBTcG9vMUKzJIVn\my3eDDpckBpfW2jbjDMeYNmdmFiMTDj[YxteyCjdDCxNk42KHWPIIDy[Ylv[3WkYYTl[EBnd3JiMTDodkBnd2yub4fl[EBjgSCUaH:tNVI{KGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFEhcHJiYomg[oxwfyCleYTvcYV1enl? M13xfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNkGzN|I1Lz5|ME[xN|MzPDxxYU6=
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PANC1 MkL5R5l1d3SxeHnjbZR6KGG|c3H5 MorKR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVGFPSzFiY3XscJMtKEeLNUC9NE4yPc7:TR?= M4XtXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzMkCwNlM3Lz5|MUKwNFI{PjxxYU6=
MDA-MB-231 MXLDfZRwfG:6aXPpeJkh[XO|YYm= MnvPR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczygS2k2OD1yLkK0{txO NXfXeVBpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GyNFAzOzZpPkOxNlAxOjN4PD;hQi=>
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MCF7 NWL5[HJRSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MoLMRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNE45P87:TR?= NUj5XYEzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1OFYyQTdpPkOxOVQ3OTl5PD;hQi=>
A549 NEDvTlBCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M{\EZ2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NVAvQDgQvF2= NUHue404RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1OFYyQTdpPkOxOVQ3OTl5PD;hQi=>
HeLa NH7vWY9CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NGXnSYtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZpkhVVSWIHHzd4F6NCCLQ{WwQVExNjh5zszN MkDsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3NE[xPVcoRjNzNUS2NVk4RC:jPh?=
DU145 MXfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MV7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKESXMUS1JINmdGy|IHL5JG1VXCCjc4PhfUwhUUN3ME2xNE45P87:TR?= MnrwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3NE[xPVcoRjNzNUS2NVk4RC:jPh?=
MDA-MB-231 NGL1W4lCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= Mmi2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGK7IF3UWEBie3OjeTygTWM2OD1zMT6y{txO MnHkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3NE[xPVcoRjNzNUS2NVk4RC:jPh?=
SiHa NXvnfXdbT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWfjV|liT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hW2mKYTDj[YxteyxiR1m1NF0xNjF{zszN NXLPe5ZZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G2NFU5PjVpPkOxOlA2QDZ3PD;hQi=>
MDA-MB-231 M4ryPWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NV73bmh4T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{whT0l3ME2wMlEz|ryP M3;aPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNkC1PFY2Lz5|MU[wOVg3PTxxYU6=
PANC1 NEjoOohIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnmxS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gVGFPSzFiY3XscJMtKEeLNUC9NE4yOs7:TR?= NV\PfHR6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G2NFU5PjVpPkOxOlA2QDZ3PD;hQi=>
MCF7 NWrM[lZXSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NGrnTlRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZpkh\my3b4Lld4NmdmOnIHLhd4VlKH[rYXLs[UBk\WyuIHPveY51cW6pIH3leIhw\CxiSVO1NF0zPc7:TR?= M{f6d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNkGwN|c5Lz5|MU[xNFM4QDxxYU6=
M-HeLa NYfFPYt[SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NH6yRYpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF2tTIVN[SClZXzsd{BjgSCobIXvdoV{[2WwY3WgZoF{\WRidnnhZoxmKGOnbHygZ492dnSrbnegcYV1cG:mLDDJR|UxRTJ6zszN MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTZzMEO3PEc,OzF4MUCzO|g9N2F-
Chang M4fOZmN6fG:2b4jpZ4l1gSCjc4PhfS=> M{nQVWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNp[W6pIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDifUBndHWxcnXzZ4Vv[2ViYnHz[YQhfmmjYnzlJINmdGxiY3;1cpRqdmdibXX0bI9lNCCLQ{WwQVQzNjIQvF2= NVflVJcyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G2NVA{PzhpPkOxOlExOzd6PD;hQi=>
MCF7 M1rremN6fG:2b4jpZ4l1gSCjc4PhfS=> MmrWOFghcHK| NXjZU3R1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgbY5kfWKjdHXkJIZweiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTJ{LkdOwG0> M4TkRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNkKwNlI2Lz5|MU[yNFIzPTxxYU6=
RAW264.7 NULGWWxbS3m2b4TvfIlkcXS7IHHzd4F6 MnzINlQhcHK| NHXXWmtEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgNlQhcHK|IHL5JI5mfXS{YXygdoVlKGS7ZTDiZZNm\CCjc4PhfUwhS0N3ME2xNU4y|ryP Ml;DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF5MEO4NVgoRjNzN{CzPFE5RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-AKT / AKT ; 

PubMed: 30759864     


MCF-7 cells were treated with 2 μM tamoxifen and harvested at the indicated time point to analyze the expressions of p-AKT and AKT by western blot.

ER-α66 / ER-α36 / HER2 / EGFR ; 

PubMed: 25203051     


Western blot analysis of the expression levels of ER-α66, ER-α36, HER2 and EGFR in ER-positive breast cancer MCF7 (A), T47D (B) and H3396 (C) cells treated with 1 µM of tamoxifen (TAM) for indicated time period.

p-AMPK / AMPK / p-ACC / ACC / p-P70S6K / p-S6 / p-4EBP1 ; 

PubMed: 27020861     


B. Immunoblot of AMPK pathway activation following treatment with 30μM tamoxifen in MCF7 and C. in MDA-MB-231 cells. 

CD36 / p-ERK / ERK ; 

PubMed: 30573731     


MCF-7 cells MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h, or with 5 μM Tam for the indicated times. Expression of CD36, p-ERK1/2 and ERK1/2 was determined by western blot.

30759864 25203051 27020861 30573731
Immunofluorescence
CD36; 

PubMed: 30573731     


MCF-7 cells were treated with tamoxifen (Tam) at the indicated concentrations for 16 h. Expression of CD36 was determined by immunofluorescent staining . 

30573731
体内研究

在肿瘤中,Tamoxifen处理快速增殖的,雌二醇激活的MCF-7异种移植通过显著降肿瘤细胞增殖引起剂量依赖性的肿瘤生长阻滞或停止。Tamoxifen处理导致肿瘤生长减慢(肿瘤倍增时间,12天),显著延长了肿瘤倍增时间Tpot) (6.6天),降低标记指数(%LI) (至8%)至处理后23天,而未处理的小鼠倍增时间是5天,Tpot是2.3 天,%LI 为23%。[5] Tamoxifen根据种属,组织和基因的不同,不仅具有抗雄性激素活性,还具有雌激素活性。Tamoxifen对骨和血清脂类浓度和子宫内膜等刺激有保护作用。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[3]

- 合并

竞争性结合试验:

从150平方厘米的T型瓶中收集细胞,蛋白以约2 mg/mL的浓度溶于PBS。180,000 ×g离心,上层清夜与不同浓度的Tamoxifen 和 2.5 nM [3H]雌二醇在0-4℃孵育16小时。用葡聚糖活性炭[l0 μL 0.5%葡聚糖 C-5% Norite A 的TE缓冲液]测定游离的类固醇。800×g,30分钟离心后测定。化合物的相对结合活性是以抑制50%放射性标记的雌二醇的浓度计算的,化合物对雌二醇的结合活性默认为100%。
细胞实验:

[3]

- 合并
  • Cell lines: 人乳腺癌细胞MCF-7
  • Concentrations: ~1 μM
  • Incubation Time: 10天
  • Method:

    MCF-7细胞接种于T-25烧瓶(1.5×105细胞/烧瓶)并在MEM中生长2天,MEM中含10 mM HEPES缓冲液,庆大霉素(50 微克/毫升),青霉素(100 单位/毫升),链霉素(0.1 毫克/毫升),牛胰岛素(6 纳克/毫升),氢化可的松(3.75 纳克/毫升),以及用葡聚糖被覆活性炭在55 ℃下处理45分钟除去内生激素的5% 牛血清。随后将培养基更换为上诉的MEM,除了其包含2%活性炭葡聚糖处理的小牛血清和不同浓度的Tamoxifen。培养结束时,测定细胞数目。


    (Only for Reference)
动物实验:

[5]

- 合并
  • Animal Models: 人乳腺癌异种移植MCF-7
  • Dosages: 2cm三苯氧胺胶囊
  • Administration: 皮下植入
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL (177.41 mM)
Water Insoluble
Ethanol ''''100 mg/mL
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
30% PEG400+0.5% Tween80+5% propylene glycol
30 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 563.64
化学式

C26H29NO.C6H8O7

CAS号 54965-24-1
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04200066 Not yet recruiting Drug: Temozolomide Tamoxifen Maprotiline Glioblastoma|Brain Tumor University of Rochester May 1 2021 Phase 1
NCT04433494 Not yet recruiting Drug: TY-302: capsule 25mg/50mg ; Tamoxifen: tablet10mg Breast Cancer|Solid Tumor TYK Medicines Inc October 2020 Phase 1
NCT04174352 Recruiting Drug: Tamoxifen|Diagnostic Test: FES PET/CT ERα+ Breast Cancer|ESR1 Gene Mutation University of Wisconsin Madison October 20 2020 Early Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I am wondering if the Tamoxifen (product Cat # S1972) is a 4-hydroxy Tamoxifen form or just plain Tamoxifen Citrate?

  • 回答:

    The Tamoxifen (S1972) is a prodrug, just plain Tamoxifen citrate.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID