Gossypol Acetate

For research use only. Not for use in humans.

目录号:S2303 别名: Gossypol Acetic acid, Pogosin, AT101 中文名称:棉酚,棉籽酚,棉子酚

Gossypol Acetate Chemical Structure

CAS No. 12542-36-8

Gossypol Acetate (Gossypol Acetic acid, Pogosin, AT101) 是多酚醛,可渗透细胞,是一些脱氢酶(如乳酸脱氢酶、NAD-linked enzymes)的抑制剂。

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RMB 725.55 现货
RMB 1406.43 现货
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客户使用Selleck生产的Gossypol Acetate发表文献13篇:

客户使用该产品的5个实验数据:

  • 16h after seeding HMLE RAS cells were exposed to 5 uM or 10 uM Gossypol in the presence or absence of Cisplatin for 16h. Afterwards, cells were harvested and lysates were analyzed for apoptosis by western blotting. Actin staining was used as loading control, while LC3a/b staining was used as indicator for autophagy. Accumulation of LC3a/b II indicates autophagy. Non-treated and DMSO-treated cells were used as control. Arrows are indicating the intended staining. For Bcl-xL: * indicates deamidated protein, # indicates unmodified protein. n=1.

    Georg August University Gottingen 2014 Western blotting. Gossypol Acetate purchased from Selleck.

  • Weight gain of Spodoptera littoralis larvae on combined Cry proteins and gossypol. Weight gain (mg ± SE) of 2nd instar S. littoralis after feeding for one week on diet containing different concentrations of Cry proteins, gossypol, or a combination of both. Control = diet with added PBST; C1 = Cry1Ac 0.05 + Cry2Ab2 1.6 pmol/g; C3 = Cry1Ac 0.52 + Cry2Ab2 16.4 pmol/g; C4 = Cry1Ac 1.7 + Cry2Ab2 54.5 pmol/g; G3 = 1.3 μmol/g gossypol. Asterisks indicate significant difference to the control diet using a GLM with gamma distribution followed by Dunnett`s Test (n = 53-60). *** p ≤ 0.001.

    Pest Manag Sci, 2016, 73(8):1670-1678. Gossypol Acetate purchased from Selleck.

  • H9c2 cells were incubated with indicated bioactive compounds for 2 hours prior to protein harvest. Cells were lysed and acetyl-lysine (Ac-Lysine), acetyl-histone 3 (Ac-H3 K9/14) and total histone 3 (Total-H3) were assessed via immunoblot. Acetylation of lysine and Ac-H3 (K9/14) increased for gossypol. Acetyl proteins were normalized to total H3 and quantitation performed via Image J software.

    Mol Nutr Food Res, 2017, 61(4). Gossypol Acetate purchased from Selleck.

  • Western blot analysis of apoptotic markers of H1975 cells after treatment of gossypol for 24 h.

    Front Pharmacol, 2018, 9:728. Gossypol Acetate purchased from Selleck.

  • Effect of AT-101 (AT) on MM cell lines survival. The survival of human (MM-B1, H-Meso-1, and MM-F1) and mouse (#40a) MM cell lines were assessed by the SRB assay after 24, 48, and 72 h of treatment with DMSO or AT-101. The percentage of surviving cells treated with the compound was calculated by normalizing the OD value to that of the control cultures (DMSO). The results are expressed as the means ± SD of three independent experiments performed in triplicate (xp ≤ 0.05, ∗p ≤ 0.01, #p ≤ 0.001 compared with the cultures treated with DMSO).

    Front Pharmacol, 2018, 9:1269. Gossypol Acetate purchased from Selleck.

产品安全说明书

Dehydrogenase抑制剂选择性比较

生物活性

产品描述 Gossypol Acetate (Gossypol Acetic acid, Pogosin, AT101) 是多酚醛,可渗透细胞,是一些脱氢酶(如乳酸脱氢酶、NAD-linked enzymes)的抑制剂。
靶点
Dehydrogenase [1] Bcl2 [5]
体外研究

Gossypol, a known antispermatogenic agent from the cotton plant genus Gossypium, was found to inhibit yellow perch sperm motility in vitro and lactate dehydrogenase activity in spermatozoa when used in a dose-dependent manner[1]. Gossypol has been approved to have antiproliferative and apoptosis-inducing effects on some kinds of cancer cell lines in vitro[2].

体内研究 Gossypol acetate is able to inhibit tumor growth in Wus1-bearing mice, but the survival of mice is not prolonged, and tumor grows rapidly after short inhibition. Gossypol has now been found to have inhibitory effects on proliferation or to induce apoptosis in ovarian cancer, endometrial cancer, adrenal cortical tumor, thyroid cancer, lung cancer, colon carcinoma, leukemia, pancreatic cancer, melanoma and lymphoma. In addition, gossypol can increase the sensitivity of drug-resistant tumor cells to chemotherapy and radiotherapy. Some clinical trials showed gossypol is well-tolerated, and partial responses are observed in some patients[4].

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[4]

- 合并
  • Cell lines: Human MM cell lines U266 and Wus1
  • Concentrations: 1, 5, 10, 25 and 50 µmol/l
  • Incubation Time: 0, 24, 48 and 72 h
  • Method:

    MM(Multiple myeloma) cells are plated in 24-cell culture clusters at a density of 1x105 viable cells/l per well. Triplicate wells are treated with 1, 5, 10, 25 and 50 µmol/l gossypol acetate, and the negative control group is supplemented with 0.1% DMSO. Then, cell numbers at different treatment time points (0, 24, 48 and 72 h) are determined by using a hemocytometer and the trypan blue dye-exclusion method. The trypan blue dye-exclusion method is used to evaluate the cell viability. The cells are examined in a counting chamber under a light microscope. Only viable cells are recorded.


    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL (172.82 mM)
Ethanol 10 mg/mL (17.28 mM)
Water Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 578.61
化学式

C32H34O10

CAS号 12542-36-8
储存条件 粉状
溶于溶剂
别名 Gossypol Acetic acid, Pogosin, AT101

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    S2812 and S2303 share same structures. However, S2812 is described as ‘R’ enantiomer. What’s the difference between S2812 and S2303?

  • 回答:

    S2303 is the mixture of S2812 and its enantiomer.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID