Nocodazole

For research use only. Not for use in humans.

目录号:S2775 别名: Oncodazole, R17934 中文名称:诺考达唑

Nocodazole Chemical Structure

CAS No. 31430-18-9

Nocodazole (Oncodazole, R17934)是一种微管聚合的快速可逆抑制剂,也抑制AblAbl(E255K)Abl(T315I),对应的IC50值分别为0.21 μM,0.53 μM和0.64 μM。Nocodazole可提高CRISPR介导的同源重组效率,对增强精确的基因编辑效力具有加成作用。Nocodazole可诱导凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 794.43 现货
RMB 580.33 现货
RMB 2217.08 现货
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客户使用Selleck生产的Nocodazole发表文献59篇:

产品安全说明书

Microtubule Associated抑制剂选择性比较

生物活性

产品描述 Nocodazole (Oncodazole, R17934)是一种微管聚合的快速可逆抑制剂,也抑制AblAbl(E255K)Abl(T315I),对应的IC50值分别为0.21 μM,0.53 μM和0.64 μM。Nocodazole可提高CRISPR介导的同源重组效率,对增强精确的基因编辑效力具有加成作用。Nocodazole可诱导凋亡。
靶点
Microtubules [2]
(Cell-free assay)
Abl [1]
(Cell-free assay)
Abl (E255K) [1]
(Cell-free assay)
Abl (T315I) [1]
(Cell-free assay)
0.21 μM 0.53 μM 0.64 μM
体外研究

Nocodazole是癌症相关激酶的高亲和力配体,如的磷酸化ABL, c-KIT, BRAF, 和 MEK ,Kd值分别为0.091 μM, 1.6 μM, 1.8 μM 和1.6 μM,。此外,Nocodazole对ABL(E255K)磷酸化, ABL(T315I) 磷酸化, BRAF(V600E) 和 PI3Kγ 的Kd值分别是0.12 μM, 0.17 μM, 1.1 μM 和1.5 μM。Nocodazole诱导慢性淋巴细胞白血病细胞凋亡;抑制胰岛素刺激的葡萄糖转运;在人类结肠癌细胞减少细胞凋亡;损害内侧神经节嵴细胞的形态和方向迁移[1]。Nocodazole于高浓度时,在细胞中迅速解聚微管,而低浓度可抑制微管的动态不稳定[2]。用Nocodazole处理经同步化6小时的分裂期细胞,加入不同浓度的紫杉醇后,可造成G1期阻滞,半数抑制浓度为4nM。紫杉醇处理经Nocodazole预处理的细胞,在无Nocodazole的条件下,细胞仅能形成游离微管,而在有Nocodazole存在的条件下,则可形成聚集的中心体微管[3]。 Nocodazole通过结合β-微管蛋白破坏微管,可阻碍二硫键形成和囊泡运输,抑制METH诱导的细胞凋亡和溶酶体功能障碍。Nocodazole预处理可显著减少METH诱导的细胞凋亡[4]。在iPSC中,Nocodazole可使HDR效率加倍,可高达30%[6]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 murine leukemia cell MmS1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1K3S2lvKH[rdILvJIZweiCrdIOgbY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGwyOjFyIH31dolv\SCuZYXr[Y1q[SClZXzsJIdzd3e2aDygTWM2OD1|ODDuUS=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzN6MUS2K|4yPzN6MUS2QE9iRg>?
LT12 MYrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Moe2OFghcHK| MWDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IILheEBNXDF{IHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUCgQUAxNjByNjFOwG0v MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ{MECxPEc,OTl{MkCwNVg9N2F-
HepG2 MWDGeY5kfGmxbjDhd5NigQ>? M2jrUFAvOSC2bzCxNEB2VQ>? NEKzcJcyKGi{ NVrZVnpRUW6qaXLpeIlwdiCxZjDUUmZidHCqYT3pcoR2[2WmIF7Gb4FxeGGEIDj1cotvd3ewIH;ybYdqdilvZHXw[Y5l\W62IITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCneIDy[ZN{\WRiaX6gbJVu[W5iSHXwS|Ih[2WubIOgZZQhOC5zIITvJFExKHWPIHnuZ5Vj[XSnZDDmc5IhOSCqcjDwdolweiC2bzDUUmZidHCqYTDpcoR2[3Srb36gcYVie3W{ZXSgZYZ1\XJiNjDodpMh[nliZIXhcE1tfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[Xl? M1rrN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK3OVIzLz5{NUiyO|UzOjxxYU6=
PANC1 MknkRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NGHPe|g1QCCqcoO= NXPnXoZ7SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDQRW5EOSClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5MEBIUTVyIE2gNE4xOSEQvF2u NGD4eYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[4PFE6Oyd-Mke2PFgyQTN:L3G+
HeLa NF\uRm5CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= Mm\GOFghcHK| NGnUeZJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygS2k2OCB;IECuNFEh|ryPLh?= NVfUU2p[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2PFgyQTNpPkK3Olg5OTl|PD;hQi=>
MDA-MB-231 MX7BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MlrZOFghcHK| MV7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3MDC9JFAvODFizszNMi=> NFe5V4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[4PFE6Oyd-Mke2PFgyQTN:L3G+
A549 Ml\mRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NXe1R5pMPDhiaILz M13GcGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJINmdGxicILvcIln\XKjdHnvckBie3OjeTygS2k2OCB;IECuNFEh|ryPLh?= NFm1T4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC4PVI{Oyd-MkmwPFkzOzN:L3G+
HeLa M3fZSmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NXzYb2d2PDhiaILz MnnMRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKZVzhJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZ4VtdCCycn;sbYZmemG2aX;uJIF{e2G7LDDHTVUxKD1iMD6wNUDPxE1w MmezQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyOEmyN|MoRjJ7MEi5NlM{RC:jPh?=
MDA-MB-231 NFPQN5dCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MmnWOFghcHK| Mn7ERY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiY3XscEBxem:uaX\ldoF1cW:wIHHzd4F6NCCJSUWwJF0hOC5yMTFOwG0v NH70[Iw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUC4PVI{Oyd-MkmwPFkzOzN:L3G+
HeLa NGHSUpJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NYjwS41pPDhiaILz NV\Rd2c5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGdKPTBiPTCwMlAyPSEQvF2u M2nydlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK3OVIzLz5{NUiyO|UzOjxxYU6=
MDA-MB-231 NH\lfW1CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NVzuNFk1PDhiaILz MnvVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEeLNUCgQUAxNjBzNzFOwG0v M3zKRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEK3OVIzLz5{NUiyO|UzOjxxYU6=
A549 M{HWWmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NVzD[2lmPDhiaILz MnnkRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFfJOVAhRSByLkCyJO69VS5? MoC5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6Mke1NlIoRjJ3OEK3OVIzRC:jPh?=
L1210 MX\GeY5kfGmxbjDhd5NigQ>? M1XZPVQ5KGi{cx?= M4K2NmlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IIDyc4xq\mW{YYTpc44hd2ZiY4XseJVz\WRibIntdIhwcWRibHX1b4VucWFiTEGyNVAh[2WubIOg[JVzcW6pIES4JIhzeyxiSVS1NEA:KDBwMEK3JO69VS5? NYPocJhDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxN{GzNVQ5Oyd-N{GzNVQ5OzxxYU6=
PANC1 NWDPN4llSW62aXPhcoNmeiCjc4PhfS=> MWW0PEBpenN? NEW2cpVCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gVGFPSzFiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHNTSiCjc4PhfUwhT0l3MDC9JFAvODJ7IN88UU4> MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzF|MEO1O{c,OjdzM{CzOVc9N2F-
A549 M2fiZ2N6fG:2b4jpZ4l1gSCjc4PhfS=> MmTrNlQhcHK| M3vOfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[W[2ZYKgNlQhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvODN4NjFOwG0v M{PvfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUGwNVM4Lz5{MEGxNFE{PzxxYU6=
NCI60 NFnhZo9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3HkXFQ5KGi{cx?= MlXLS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUmNKPjBiY3XscJMhcW6ldXLheIVlKG[xcjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiR1m1NEA:KDBwMEO4PUDPxE1w NWTz[ndIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke5OVYxOzhpPkK3PVU3ODN6PD;hQi=>
P388 MmLBRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NXzxbG5NPDhiaILz NUPmfG5PSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBud3W|ZTDQN|g5KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDYWHQh[XO|YYmsJGlEPTBiPTCwMlA1KM7:TT6= NIqxN3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUKyNFAyQCd-MUmyNlAxOTh:L3G+
LT12 MUTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M4f5NlQ5KGi{cx?= M3HoNmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgUHQyOiClZXzsd{BjgSC[VGSgZZN{[XliYX\0[ZIhPDhiaILzMEBKSzVyIE2gNE4xPCEQvF2u MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF6MUG2OEc,OTdzOEGxOlQ9N2F-
LT12 MYjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MV20PEBpenN? M4\nb2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgdoF1KEyWMUKgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFiWVDDhd5NigSxiSVO1NEA:KDBwMESg{txONg>? MnfMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3M{e3OlUoRjJyNUO3O|Y2RC:jPh?=
LT12 NX3xUFA6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NELUbVE1QCCqcoO= M1:2OGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgdoF1KEyWMUKgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFiWVDDhd5NigSxiSVO1NEA:KDBwMESg{txONg>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdyNUKyN{c,OjF5MEWyNlM9N2F-
MDA-MB-231 NIH3fYhCdnSrY3HuZ4VzKGG|c3H5 NUfz[4pUPDhiaILz NH\WeZFCdnSrY3HuZ4VzKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDHTVUxKD1iMD6wOFIh|ryPLh?= NFLYbGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GzNFM2Pyd-MkexN|A{PTd:L3G+
L1210 M2ruPWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M3P6XFQ5KGi{cx?= MWTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFyxNlExKGOnbHzzJIJ6KFiWVDDhd5NigSCjZoTldkA1QCCqcoOsJGlEPTBiPTCwMlA2KM7:TT6= NHrndlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G4NVE3PCd-MUexPFEyPjR:L3G+
P388 MnTBRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MUG0PEBpenN? NGPHd2pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JI1wfXOnIHHkdolidXmlaX6tdoV{cXO2YX70JHA{QDhiY3XscJMh[W[2ZYKgOFghcHK|IHL5JHhVXCCjc4PhfUwhUUN3MDC9JFAvODVizszNMi=> NFzCW4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m3N|M3OSd-MUe5O|M{PjF:L3G+
L12 NFy1R4hCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NXjHcFNOPDhiaILz MWnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IILheEBufWy2aXTyeYcuemW|aYP0ZY51KExzMjDj[YxteyCjZoTldkA1QCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwJF0hOC5yNTFOwG0v NYfLV3pKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5O|M{PjFpPkG3PVc{OzZzPD;hQi=>
L1210 MlLNRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NFyyN3Y1QCCqcoO= MlfoRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCOMUKxNEBk\WyuczDh[pRmeiB2ODDodpMh[nliWGTUJIF{e2G7LDDJR|UxKD1iMD6wOUDPxE1w NY\qfZpKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1N|c4PjVpPkKwOVM4PzZ3PD;hQi=>
P388 MmH4RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NXfFdVM{PDhiaILz M{TCUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgRWRTNXKnc3nzeIFvfCCvb4Xz[UBROzh6IHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUCgQUAxNjB3IN88UU4> M1jCXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{C1NlI{Lz5{MUewOVIzOzxxYU6=
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SK-N-MC Ml3qdWhVWyCjc4PhfS=> NU[4[mpreUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-12 MmHGdWhVWyCjc4PhfS=> M{G4U5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTF{IHPlcIx{ MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
LAN-5 MWDxTHRUKGG|c3H5 MlfOdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUGFPNTViY3XscJM> MoLKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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MG 63 (6-TG R) NHe3XVRyUFSVIHHzd4F6 Ml3xdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUWchPjNiKE[tWGchWiliY3XscJM> MljPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
U-2 OS MmG2dWhVWyCjc4PhfS=> NVXmNIt6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXS1{IF;TJINmdGy| NHXUbIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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Saos-2 MVfxTHRUKGG|c3H5 NFOwXFNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBU[W:|LUKgZ4VtdHN? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
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PC3MLN4 NFPRbZBHfW6ldHnvckBie3OjeR?= NIq1fZUyKHWP NXzh[GxHPDhiaILz NUO3VI44TGm|coXweIlwdiCxZjDtbYNzd3S3YoXs[UBlgW6jbXnjd{BqdiCqdX3hckBRSzOPTF60JINmdGy|IHH0JFEhfU1iYX\0[ZIhPDhiaILzJIJ6KGmvbYXuc4ZtfW:{ZYPj[Y5k\SCvaXPyc5Nkd3CrYzDt[ZRpd2R? NIW0[G09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUK4PFk{QSd-MkmyPFg6Ozl:L3G+
MCF7 NELJXWdHfW6ldHnvckBie3OjeR?= NUjIR3lVOSC3TR?= NHrIR3A1KGi{cx?= Mnr4TY5lfWO2aX;uJI9nKHS3YoXsbY4h\GWyb3z5cYVzcXqjdHnvckBqdiCqdX3hckBOS0Z5IHPlcIx{KGG2IEGgeW0h[W[2ZYKgOEBpenNiYomgW4V{fGW{bjDicI91KG2ndHjv[C=> NHuyR3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[4PVczQCd-Mke2PFk4Ojh:L3G+
Jurkat MUXGeY5kfGmxbjDhd5NigQ>? NHnwTFYyKHWP M1\hNFQhcHK| NYLxTGpNUW6mdXP0bY9vKG:oIIT1ZpVtcW5iZHXwc4x6dWW{aYrheIlwdiCrbjDoeY1idiCMdYLrZZQh[2WubIOgZZQhOSC3TTDh[pRmeiB2IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:m M2jSTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Nki5O|I5Lz5{N{[4PVczQDxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cdc2 / cyclin B1 / MAD2 / Cdc20 ; 

PubMed: 21918689     


E (upper part). Time-dependent changes in cyclin B1 and Cdc2 protein levels following nocodazole treatment. Cells were treated with nocodazole (250 nM) for the length of time as indicated, and whole cell lysates were prepared. An equal amount of protein lysates was electrophoretically separated on the 10% SDS-polyacrylamide gel, and transferred to nitrocellulose membrane. Western blots were detected using specific antibodies against cyclin B1, Cdc2 (CDK1), MAD2, and Cdc20 on an enhanced chemiluminescence (ECL) apparatus. Membrane was stripped for determining the levels of GAPDH as a loading control. E (lower part). The relative protein levels for cyclin B1, Cdc2, MAD2, and Cdc20 were calculated according to their densitometry readings, which were normalized according to the corresponding readings for the GAPDH protein bands. Each value is mean ± S.D. from three replicate determinations. * P<0.05, ** P<0.01 versus vehicle-treated control. 

21918689
Immunofluorescence
Tubulin / LC8 ; 

PubMed: 23038268     


Shown is endogenous LC8 colocalized with microtubules in HeLa cells. Control, 20 nm taxol- and 100 nm nocodazole-treated cells were stained with α-tubulin and LC8 antibodies. Scale bar, 20 μm.

Brd4 / α-tubulin ; 

PubMed: 22567088     


Cells were treated with indicated concentrations of nocodazole for 4 h, and mitotic cells were immunostained for endogenous Brd4 and α-tubulin. DNA was stained by Hoechst33324.

23038268 22567088
Growth inhibition assay
Cell viability ; 

PubMed: 21918689     


Changes in cell viability (MTT assay) after treatment of cells with 250 nM nocodazole for different lengths of time. Each data point is the mean ± S.D. from four replicate measurements from one representative experiment. 

21918689
体内研究 Ketoconazole和Nocodazole联合使用比单独使用可更强地抑制小鼠的肿瘤体积和肿瘤重量。在COLO205肿瘤移植模型中联合使用的诱导凋亡效果也比单独使用更明显[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

动物实验:

[5]

- 合并
  • Animal Models: COLO-205肿瘤移植裸鼠
  • Dosages: 5 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 7 mg/mL (23.23 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
1mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 301.32
化学式

C14H11N3O3S

CAS号 31430-18-9
储存条件 粉状
溶于溶剂
别名 Oncodazole, R17934

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

Microtubule Associated Signaling Pathway Map

相关Microtubule Associated产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID