Ponatinib (AP24534)

目录号:S1490 中文名称:帕纳替尼

仅限科研使用

Ponatinib (AP24534)是一种新型有效的多靶点抑制剂,在无细胞试验中作用于Abl,PDGFRα,VEGFR2,FGFR1和Src,IC50分别为0.37 nM,1.1 nM,1.5 nM,2.2 nM和5.4 nM。Ponatinib (AP24534)可抑制自噬。

Ponatinib (AP24534) Chemical Structure

CAS: 943319-70-8

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 819.31 现货
RMB 903.01 现货
RMB 3034.48 现货
RMB 7959.08 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Ponatinib (AP24534)发表文献181篇:

产品安全说明书

Bcr-Abl抑制剂选择性比较

相关Bcr-Abl产品

生物活性

产品描述 Ponatinib (AP24534)是一种新型有效的多靶点抑制剂,在无细胞试验中作用于Abl,PDGFRα,VEGFR2,FGFR1和Src,IC50分别为0.37 nM,1.1 nM,1.5 nM,2.2 nM和5.4 nM。Ponatinib (AP24534)可抑制自噬。
靶点
Abl [1]
(Cell-free assay)
PDGFRα [1]
(Cell-free assay)
VEGFR2 [1]
(Cell-free assay)
FGFR1 [1]
(Cell-free assay)
c-Src [1]
(Cell-free assay)
点击更多
0.37 nM 1.1 nM 1.5 nM 2.2 nM 5.4 nM
体外研究

Ponatinib是有效的可用于口服的多靶点激酶抑制剂,作用于野生型细胞,及T315I 突变细胞IC50分别为1.2和8.8nM。[1] Ponatinib抑制Ba/F3衍生细胞系的增殖。[2] Ponatinib作用于表达野生型或T315I突变型BCR-ABL的CML细胞系,有效抑制BCR-ABL调节的信号。Ponatinib阻断在细胞瘤筛选时产生抗性突变。[3] Ponatinib也抑制VEGFR和FGFR家族激酶,在体外抑制FLT3, KIT, FGFR1,和PDGFRa激酶活性时IC50分别为13, 13, 2, 和1 nM.[4]Ponatinib抑制全部4种RTKs磷酸化,存在剂量依赖性,IC50值为0.3到20 nM。然而,Ponatinib抑制表达野生型FLT3的RS4;11细胞时IC50>100 nM。

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
EoL-1-cell NEfNO2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;TNmlEPTB;MD6wNFAxODByM{W5JO69VQ>? MlfsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
MV-4-11 Mn;NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWj5VJVsUUN3ME2wMlAxODB7NU[g{txO NX71VpB4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
MEG-01 NVyxc4ZwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX\JR|UxRTBwMECwNVI5KM7:TR?= MnvBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
LAMA-84 MnToS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX[wcldQUUN3ME2wMlAxODF4NTFOwG0> NYDISVU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
CGTH-W-1 M3rUe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTBwMECxOlkh|ryP NVficY9ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
KASUMI-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF2zWoZKSzVyPUCuNFAzOTdizszN NUe1UJFCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
BE-13 NXnZPYVpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEfXVlhKSzVyPUCuNFAzPTlizszN NIjOU4E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
CTV-1 MkezS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwMEC1NlEh|ryP MleyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
NKM-1 NVPib29jT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX;OV4VGUUN3ME2wMlAxPjV3IN88US=> NYT4UIpURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
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KU812 NFHYepJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfaTWM2OD1yLkCyPFQ5KM7:TR?= NVXUTGpLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
KG-1 MmjhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33POmlEPTB;MD6wN|I6PCEQvF2= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
DOHH-2 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfKb2U{UUN3ME2wMlA1QDB|IN88US=> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
HuO9 NUPpNIFxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml74TWM2OD1yLkC1OFQ5KM7:TR?= M1zMS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK|5USU6JRWK8M4E,
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SU-DHL-1 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTBwME[zO|kh|ryP MmTUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
NCI-H719 NGnySmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTBwMEe4PFgh|ryP M{DlXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK|5USU6JRWK8M4E,
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PC-3 NHjkTIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXjJR|UxRTR2LkSzOFch|ryP M2HnT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK|5USU6JRWK8M4E,
HuP-T4 NWPYSJNRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HvU2lEPTB;NESuOFQ4PCEQvF2= MmPlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
NCI-H510A MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVnJR|UxRTR2LkewPEDPxE1? NX7HfGdrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
LB2518-MEL M4e4UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vOOGlEPTB;NESuO|k{PyEQvF2= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
TE-5 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV72dWVrUUN3ME20OU46PThzIN88US=> MnjLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
EW-24 NVLkN2pHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVqxU|dXUUN3ME20Ok4yPzJ|IN88US=> NUnvNmZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
LB647-SCLC MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MW\JR|UxRTR4LkKxOVUh|ryP MnTqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
NCI-H524 M{L1SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PZZ2lEPTB;NE[uO|E5PSEQvF2= MmTiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
EW-11 M1;xO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;0VGRsUUN3ME20Ok46Ozd|IN88US=> Mn\0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
KALS-1 Mnf4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTG[ldKSzVyPUS3MlIxPjZizszN NX;oXGx5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
BT-474 M{Xibmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\STWM2OD12Nz60NlE3KM7:TR?= M{ixZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK|5USU6JRWK8M4E,
SK-N-AS NV3tc5R[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fMZWlEPTB;NEeuPFg5KM7:TR?= M3KyNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK|5USU6JRWK8M4E,
HCC2157 MlHkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfBTWM2OD12OD6wO|Yh|ryP NFTyV5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
HCC1954 NGrQSmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnR[W9XUUN3ME20PE4yPzB2IN88US=> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
FADU Ml;WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjIRnJ5UUN3ME20PE4{ODNzIN88US=> M1[yeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEyPzF6M{evK|5USU6JRWK8M4E,
S-117 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVq5NIVKUUN3ME20PE4{QDF3IN88US=> NUj1OZJuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTF5MUizO{8oRlODTlfFVlww[T5?
LS-1034 MlzYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoO4TWM2OD12OD63NFY4KM7:TR?= MkPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVE4OTh|Nz:nQnNCVkeHUkyvZV4>
CAMA-1 MkfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1;vNWlEPTB;NEmuOVU1OyEQvF2= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
LU-134-A NXjJcW15T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\ydGVKSzVyPUS5MlgxQDVizszN NFjqVGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOTdzOEO3M{c,W0GQR1XSQE9iRg>?
NCI-H1838 Ml;YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fTOWlEPTB;NEmuPFA5PyEQvF2= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNVcyQDN5Lze+V2FPT0WUPD;hQi=>
Assay
Methods Test Index PMID
Western blot ABL β-catenin β-catenin Mcl-1 / Survivin / Bcl-Xl / XIAP / Bax / Bcl-2 / Bim AIF / Cyto C PARP / Caspase-3 / Active Caspase-3 p-PDGFRα / PDGFRα PI3K p85 / p-PI3K / p-PDK1 / PDK1 / p-mTOR / mTOR p-JAK2 / JAK2 / p-STAT3 / STAT3 p-p38 MAPK / p38 MAPK c-Jun / p-JNK(T183/Y185) / JNK p-MEK / MEK / p-ERK / ERK RAD51 p-Rb(S807/811) / Rb / CDK4 / CDK6 / Cyclin D1 / CDK2 / Cyclin E1 cleaved caspase-3 / cleaved caspase-7 / BAX / Bcl-2 pFGFR1(pY20) / FGFR1 pSrc / Src / pPlcg / Plcg / pStat5 / Stat5 19878872 24472312 30959969 22781593
Growth inhibition assay Cell viability 30174789
体内研究 Ponatinib抑制取自FLT3-ITD阳性AML病人的原代白血病细胞,而不抑制表达阴性FLT3的AML病人的白血病细胞。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
  • 激酶实验:

    通过同质时间分辨荧光共振能量转移法(TR-FRET),使用纯化的人类野生型ABL激酶,T315I突变型ABL激酶,及生物素化的磷酸激酶底物肽: 生物素-EKKMAAEAIYAAPFAK-NH2,测定野生型ABL和T315I突变型ABL活性的抑制激酶反应在全LANCE激酶buffer (LKB)中进行,LKB包括20 mM NaHEPES(pH 为7.4), 0.1 mg ⁄ mL BSA, 1 mM ATP, 10 mM MgCl2, 及0.41 mM DTT,在提前用1% BSA(溶于PBS)包被的96孔板上4oC下过夜。用于激酶反应,Ponatinib稀释物和野生型ABL, T315I突变型ABL (40 pM), 及底物肽(50 nM), 在室温下温育1小时(T315I突变型ABL)或2小时(野生型ABL)。加入15 nM 抑制剂, 6 nM 铕标记的磷酸酪氨酸抗体(磷酸化位点在第66为酪氨酸上),及60 nM 别藻蓝素标记的链酶亲和素,终止反应。实验板在室温下黑暗温育20分钟,使用Wallac Victor2V读数器读取615和665nm处的荧光值。在孔中数据平均值中插入抑制剂的3倍连续稀释液,再读取665nm处荧光值,计算IC50值。

细胞实验:[1]
  • Cell lines: Ba ⁄ F3细胞
  • Concentrations: 0-625 nM, 和0-10 µM
  • Incubation Time: 72小时
  • Method: Ba/F3细胞系按每孔4×103个细胞分布在96孔板上(4×103),和梯度浓度Ponatinib温育72小时。Ponatinib浓度范围为: 0-625 nM 作用于表达BCR-ABL细胞,0-10 µM 用于测定BCR-ABL阴性细胞。使用MTS实验测定细胞增殖,取三次平行实验平均值测定IC50值。
动物实验:[3]
  • Animal Models: 皮下植入MV4-11细胞移植瘤(1×107,50%基质胶中) 的雌性CB.17 SCID鼠
  • Dosages: 每天处理一次,持续4周
  • Administration: 口服饲喂处理

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
1% CMC Na

30mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 532.56
化学式

C29H27F3N6O

CAS号 943319-70-8
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05306301 Not yet recruiting Drug: Ponatinib Chemotherapy|Leukemia Acute Lymphoblastic Gruppo Italiano Malattie EMatologiche dell''Adulto September 1 2022 Phase 2
NCT05268003 Recruiting Drug: Ponatinib|Drug: Venetoclax|Drug: Mini-hyper CVD Acute Lymphoblastic Leukemia|Leukemia M.D. Anderson Cancer Center June 7 2022 Phase 2
NCT04554459 Recruiting Drug: Ponatinib 15 MG Oral Tablet Acute Lymphoblastic Leukemia Adult|Ph+ ALL|Newly Diagnosed Institute of Hematology and Blood Transfusion Czech Republic|CZECRIN - Czech Clinical Research Infrastructure Network February 16 2021 Phase 2
NCT04160546 Recruiting Drug: Ponatinib 15 MG|Drug: Acetylsalicylic acid 100 MG Chronic Myeloid Leukemia Chronic Phase Fundacion CRIS de Investigación para Vencer el Cáncer|Incyte Biosciences UK|Apices Soluciones S.L. January 17 2020 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技术支持

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操作手册

如果有其他问题,请给我们留言。

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常见问题及建议解决方法

问题 1:
Is the pre-solubilized inhibitor suitable for in vivo whole animal studies? Whether the solvent contains any non-DMSO stabilizer?

回答:
Our 10mM Ponatinib in DMSO solution is in pure DMSO and there is not other stabilizer added. You can use it for animal study. The vehicle we suggest for Ponatinib is: 30% PEG400/0.5% Tween80/5% Propylene glycol. It is a milky suspension used for oral gavage.

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