BI 2536

For research use only. Not for use in humans.

目录号:S1109

BI 2536 Chemical Structure

CAS No. 755038-02-9

BI-2536是一种有效的Plk1抑制剂,无细胞试验中IC50为0.83 nM。BI-2536可抑制 Bromodomain 4 (BRD4) ,其Kd值为37 nM,并有效地抑制 c-Myc 的表达。BI-2536可诱导凋亡而减弱自噬。Phase 2。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1978.87 现货
RMB 981.57 现货
RMB 5492.59 现货
RMB 8160.83 现货
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客户使用Selleck生产的BI 2536发表文献241篇:

产品安全说明书

PLK抑制剂选择性比较

生物活性

产品描述 BI-2536是一种有效的Plk1抑制剂,无细胞试验中IC50为0.83 nM。BI-2536可抑制 Bromodomain 4 (BRD4) ,其Kd值为37 nM,并有效地抑制 c-Myc 的表达。BI-2536可诱导凋亡而减弱自噬。Phase 2。
特性 BI 2536是第一个有效的Plk1选择性抑制剂,抑制 Plk1的标记。
靶点
c-Myc [3]
()
PLK1 [1]
(Cell-free assay)
PLK2 [1]
(Cell-free assay)
PLK3 [1]
(Cell-free assay)
BRD4 [3]
(Cell-free assay)
0.83 nM 3.5 nM 9.0 nM 37 nM(Kd)
体外研究

除了Plk1, BI 2536也抑制 Plk2 和Plk3活性,只是抑制程度低点,IC50分别为3.5 nM和9.0 nM。BI 2536比一组63种其他蛋白激酶(IC50>10 μM)选择性高1000多倍。BI 2536 按10 nM 到 100 nM剂量作用于HeLa细胞,抑制有丝分裂中心体中γ-微管蛋白招募和Apc6 磷酸化,抑制染色体臂中释放黏结蛋白, 诱导产生单极主轴,及一些依赖Plk1的其他有丝分裂过程。与作用于Plk1 RNAi效果相一致, BI 2536处理HeLa细胞,使细胞周期停在G2/M期, 随后DNA分解及凋亡,且累积断裂的PARP p85片段,这种作用存在浓度依赖性。BI 2536抑制一组32种人肿瘤细胞系生长,EC50为2-25 nM,抑制指数生长的hTERT-RPE1,人脐静脉内皮细胞 (HUVECs),和正常大鼠肾脏(NRK) 细胞的增殖,EC50为12-31 nM。[1] BI 2536 抑制Plk1,降低甲状腺未分化癌(ATC)细胞如 CAL62, OCUT-1, SW1736, 8505C,和 ACT-1 的生长的生长和活力,EC50为1.4-5.6 nM。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PSN1 NFLJWVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTBwMEC3O|Yh|ryP M3zBXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
ACN NWLVZpdzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nmd2lEPTB;MD6wNFg1OyEQvF2= M{X2[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
TE-8 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXP0TotZUUN3ME2wMlAxQTRzIN88US=> NVTnXWhKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
LC-2-ad MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MluyTWM2OD1yLkCxNFk{KM7:TR?= MkK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
ALL-PO M2PNSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfTWFJ4UUN3ME2wMlAyOyEQvF2= NWP5WVVkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
EW-16 Mm\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEn5O4hKSzVyPUCuNFE{OzZizszN MmHmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
KM12 M4\UXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjMTWM2OD1yLkCxN|Q5KM7:TR?= NIjMe2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
GI-1 M3zK[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn[zTWM2OD1yLkCxN|g{KM7:TR?= M{nLRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LS-411N MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4CzeWlEPTB;MD6wNVQxPyEQvF2= M{X0[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
ES8 MlvFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPBRVhKSzVyPUCuNFE1QTRizszN NHzEbWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SNU-C2B M1ixcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXS4cpRUUUN3ME2wMlAyPTJ4IN88US=> NI\4Z409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
CESS M{\mVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\UTWM2OD1yLkCxOlAzKM7:TR?= M3G5RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
697 M17jZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTBwMEG2Olgh|ryP MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H1963 NXPyXIJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTBwMEG2O|Mh|ryP NXH5OVhRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
IA-LM NEPXSmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjQTWM2OD1yLkCxOlg5KM7:TR?= NG\rU|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SW954 NVvLZnBOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlfFTWM2OD1yLkCxO|A2KM7:TR?= NHPjT409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
RL95-2 M2HIWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoDITWM2OD1yLkCxO|M5KM7:TR?= M1TI[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LXF-289 M3fV[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vnPGlEPTB;MD6wNVc4KM7:TR?= M3LkTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
OCUB-M M1nSe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFXxcWdKSzVyPUCuNFE5OjZizszN MlzaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
RKO MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTBwMEG5PFch|ryP M3HqPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LOUCY NXqwSFl1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTBwMEKwOUDPxE1? M2fwb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NOS-1 NXzCXWFuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnK1TWM2OD1yLkCyNFk{KM7:TR?= M4fU[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
A4-Fuk MlXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwMEKyNVgh|ryP NVy5bGJWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
A388 Ml\ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX24R2l3UUN3ME2wMlAzOjJ5IN88US=> NI[5V5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
TE-9 NHvCeI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjT[49KSzVyPUCuNFIzPjFizszN M2D4[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
KURAMOCHI MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXPT|ZCUUN3ME2wMlAzOjh3IN88US=> NUPzWZNmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
KALS-1 NIHkVpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnsPYlKSzVyPUCuNFI{OzhizszN NFvnSWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HOP-62 M3XQV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLNTWM2OD1yLkCyOFIh|ryP MmDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
RPMI-8402 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTBwMEK1NFgh|ryP NIfBbJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
TE-1 NIrLTGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrDWZB7UUN3ME2wMlAzPTF{IN88US=> M4XBNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
CCRF-CEM NGjne2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX\tWmpTUUN3ME2wMlAzPTR|IN88US=> MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
HUTU-80 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHPN|VWUUN3ME2wMlAzPjJ2IN88US=> MnTLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NCI-H1648 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MULJR|UxRTBwMEK2OFch|ryP NUjyb5pMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
HCE-4 MkjkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYC2PYxyUUN3ME2wMlAzPjh{IN88US=> M1v1dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
ETK-1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmOwTWM2OD1yLkCyO|M3KM7:TR?= NFP3WXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
TE-15 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfjTWM2OD1yLkCyO|M5KM7:TR?= M{OwRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
BE-13 NYH2OFdST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;vTWM2OD1yLkCyO|Qh|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
KE-37 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2TSXGlEPTB;MD6wNlc1OSEQvF2= NEPPeWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1355 NHXEbmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVzJR|UxRTBwMEK3PFMh|ryP M4PTUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
TE-11 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWjNOINSUUN3ME2wMlAzQDB7IN88US=> M3fZd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
BL-70 MlnMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmDXTWM2OD1yLkCyPFU4KM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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MOLT-4 Mnj0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGjZUINKSzVyPUCuNFg6QDNizszN NWS0dXJlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-SNU-5 NVnNbmF6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjxfZNKSzVyPUCuNFkzPThizszN NVPhdndlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
ONS-76 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHxTWM2OD1yLkC5Nlc3KM7:TR?= NXHhRXB7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H446 M1\ycGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;xOGlUUUN3ME2wMlA6PDZ{IN88US=> MlnxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
COR-L279 NGDzUI5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX3mZmJ6UUN3ME2wMlA6PTV4IN88US=> NYLhWnA6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
Calu-6 NGm4c4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTBwMEm1PVgh|ryP MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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NCI-H2126 NFHFSIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmWzTWM2OD1yLkC5O|kyKM7:TR?= NVzwWJNrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H1882 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\vTWM2OD1yLkC5PFA6KM7:TR?= MlriQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
BB65-RCC NV:zNm9ZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULLdVl3UUN3ME2wMlExOjl2IN88US=> NH\jdIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
DU-4475 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7zTWM2OD1yLkGwOFA4KM7:TR?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H526 MoDpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTBwMUC0OEDPxE1? NHr4Z289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NEC8 NGDncZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwMUC1NlUh|ryP NH3ZO2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1304 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PvVGlEPTB;MD6xNFU6PCEQvF2= MmLWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
L-540 NIjiZodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4D0WGlEPTB;MD6xNFcxQCEQvF2= NV\5SZlsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-SNU-16 NYr3e4tVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEL3S3pKSzVyPUCuNVA4PDJizszN Mn\YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
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TE-441-T M3jCdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHGTWM2OD1yLkWwNVIh|ryP NGDLO5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
MDA-MB-134-VI NGnPc2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPZRnFKSzVyPUCuOVA6OjFizszN Mlv2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
SCC-3 MnrNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPkSIpkUUN3ME2wMlUyQDd|IN88US=> MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
KLE NEPLUZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrVc|BKSzVyPUCuOVIyPzFizszN MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
LB2241-RCC MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nIfWlEPTB;MD61NlUzPSEQvF2= MkXaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NCI-H1417 NHLrZmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLQTWM2OD1yLkWzOFM2KM7:TR?= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H2081 NWDiZ2l5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3IcWNKSzVyPUCuOVg3OTFizszN MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H1522 M3PY[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTBwNUmzPFMh|ryP NV\mdpdNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H889 MmLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml34TWM2OD1yLkW5PFQ1KM7:TR?= NHLXXJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
CGTH-W-1 Mn\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXSR5RKSzVyPUCuOlA4PTVizszN NX;ySVEyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
EB-3 Mo\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwNkGxOVMh|ryP M4XKbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
D-336MG M1HIRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGntdGRKSzVyPUCuOlE4QTFizszN MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
LC-1F MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjMTWM2OD1yLk[yOVkzKM7:TR?= MkLLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
MHH-NB-11 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37Lc2lEPTB;MD62N|gxOSEQvF2= M2LUd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
SK-N-FI NVXucnB[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnflTWM2OD1yLk[zPVEh|ryP NHHtO3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H69 NYWwTmk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkXmTWM2OD1yLk[0PFc6KM7:TR?= NU[wNoM3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
SCH M{jtPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLVTXZKSzVyPUCuOlYxOjNizszN NEnEVlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
LS-513 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzUTWM2OD1yLk[3NlU6KM7:TR?= M2KzTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
CP67-MEL NEfVRnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwNke1PVYh|ryP NHnUVIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
GCIY MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXOTWM2OD1yLkewN|Y{KM7:TR?= NW\t[I84RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
SHP-77 Mlu5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTBwN{C3N|Qh|ryP MkPXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
EHEB MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXXVWVKSzVyPUCuO|E5PTRizszN MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
D-247MG M2DhZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGW1VmNKSzVyPUCuO|MzOzJizszN MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
Raji MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWjJR|UxRTBwN{S5PFkh|ryP MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
MPP-89 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fqNmlEPTB;MD63OVk4QCEQvF2= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
EKVX MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTBwN{mwO|Mh|ryP M4TYS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
HCC2218 NGPNfYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TBRmlEPTB;MD63PVE{OSEQvF2= M2LXWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
KNS-81-FD MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTBwOEGwNVEh|ryP MmO5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
HCC2157 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTBwOES5OVEh|ryP NHXLSo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
BT-474 M1zQb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVjJR|UxRTBwOEi4OVQh|ryP M2HKZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H748 NW\l[|VTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NE\xUopKSzVyPUCuPVEzKM7:TR?= NHzFc4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
EW-24 MoOwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH[wTopKSzVyPUCuPVIxODJizszN MlHpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
D-263MG NGHsPVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDYW2JKSzVyPUCuPVQ2OTJizszN MoH5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
OMC-1 NVHmcGxUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjVbpJ3UUN3ME2wMlk3Ozd|IN88US=> NGnXS4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H2196 M1Tncmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7INVJKSzVyPUCuPVc4PzdizszN MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
HAL-01 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\ieGlEPTB;MD65PVg4OiEQvF2= NGT2NGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H2107 NVLKOYJUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUD4V3NRUUN3ME2xMlAyQDV2IN88US=> NELhSZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
LB996-RCC NF2wSJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXmyb4NXUUN3ME2xMlA5ODVzIN88US=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H1395 NHHPd2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\XVmlEPTB;MT6xNFY4KM7:TR?= NIHuV2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
CHP-126 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGK5O5dKSzVyPUGuNVYyQTlizszN NIXkRmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NB69 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTFwMk[xOFgh|ryP MoHwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
CAS-1 NVrMOVVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTFwM{SyN|Eh|ryP MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
JVM-3 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVrHSGpEUUN3ME2xMlQ4QDV3IN88US=> NEmxOZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
DMS-153 M1LXOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTFwNUe2O|Qh|ryP NGXhSXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SK-MEL-2 MlTUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIT2coRKSzVyPUGuO|E4QDlizszN MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H1155 Mn3xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLjTWM2OD1zLkezOlc3KM7:TR?= Ml;tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
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SIL-ALL NHnLN2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4D4clI1KGh? MXvJR|UxRTF7OT63O{BvVQ>? M3jTXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
CEM NIG3SJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfXV44zPCCq MW\JR|UxRTV{LkG4JI5O NUnrTlhQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
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SIL-ALL NUH6NGt5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7vdXE1QCCq Mn\NTWM2OD15ND62NkBvVQ>? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
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NALM-16 NH3ISWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHvhfWk1QCCq MlzCTWM2OD14Mz6yOEBvVQ>? NIHoSFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
JURKAT NIXxfGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjVU20zPzJiaB?= NFvoRWdKSzVyPUOuOlUhdk1? MmqwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
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MG 63(6-TG R) NHX2bWNyUFSVIHHzd4F6 MUfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDNS{A3OyBqNj3US{BTMSClZXzsdy=> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Fibroblast cells MkOzdWhVWyCjc4PhfS=> MXnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDjc451em:uIFjoJJdqdGRidInw[UBncWK{b3LsZZN1KGOnbHzz M4jSdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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NB-EBc1 MlzZdWhVWyCjc4PhfS=> M{TnbpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQj3FRoMyKGOnbHzz NXnIOJZ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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Rh18 MnWxdWhVWyCjc4PhfS=> NF\MOlRyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnixPEBk\Wyucx?= MkH2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 MVXxTHRUKGG|c3H5 MlrqdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIyPjR|IHPlcIx{ MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
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SJ-GBM2 MVrxTHRUKGG|c3H5 MWrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTTk1ISk1{IHPlcIx{ MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
TC32 NIrrbVVyUFSVIHHzd4F6 NVy4UoM6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN|MjDj[Yxtew>? NVuzW|hSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh18 NWjPZ2xWeUiWUzDhd5NigQ>? NYTTTZE6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? M3;XdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 NXnqeHQ3eUiWUzDhd5NigQ>? MVPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTZY9{NTJiY3XscJM> NEXQZok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
DU145 MoroRZBweHSxc3nzJIF{e2G7 MVexNFAhdk1? M4PlV|I1KGi{cx?= NFrWSINKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFTVNVQ2KGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIGDBVnAh[2ynYY\h[4Uh[XRiMUCwJI5OKGGodHXyJFI1KGi{czDifUBqdW23bn;icI91fGmwZzDt[ZRpd2R? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NUm0NUc,OjV4OEW5OFE9N2F-
HeLa NGTGR|lHfW6ldHnvckBie3OjeR?= Ml7CNVAxKG6P NUfPUVJ3UW6qaXLpeIlwdiCxZjDQUGsyKFSqckKxNEBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCBzMECgcm0h[nliaX3teY5w[myxdITpcochdWW2aH;k MlnRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4OEW5OFEoRjJ3Nki1PVQyRC:jPh?=
DU145 MlzpSpVv[3Srb36gZZN{[Xl? NH;zOGkyODBibl2= MmG1TY5pcWKrdHnvckBw\iCSTFuxJJBzd3SnaX6gZYNkfW23bHH0bY9vKGmwIHj1cYFvKESXMUS1JINmdGy|IHH0JFExOCCwTTDifUBqdW23bn;icI91fGmwZzDt[ZRpd2R? NX73dXhLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFU6PDFpPkK1Olg2QTRzPD;hQi=>
HeLa M4DQVGZ2dmO2aX;uJIF{e2G7 NX7TWXR5OTByIH7N MkTwTY5pcWKrdHnvckBw\iCSTFuxJJBzd3SnaX6gZYNkfW23bHH0bY9vKGmwIHj1cYFvKEinTHGgZ4VtdHNiYYSgNVAxKG6PIHL5JIludXWwb3Lsc5R1cW6pIH3leIhw\A>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NUm0NUc,OjV4OEW5OFE9N2F-
HeLa NXyyTJluTnWwY4Tpc44h[XO|YYm= MmDrNVAxKG6P NIf1enIyQCCqcoO= NFn0[GVKdmS3Y4Tpc44hd2ZibX;uc5BwdGG{IH3peI91cWNic4DpcoRt\XNiaX6gbJVu[W5iSHXMZUBk\WyuczDheEAyODBibl2gbY5kfWKjdHXkJIZweiBzODDodpMh[nliaX7kbZJm[3RiaX3teY5w\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcIm= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NUm0NUc,OjV4OEW5OFE9N2F-
HeLa MUfBdI9xfG:|aYOgZZN{[Xl? NVXBV|B3OTByIH7N MXWyOEBpenN? MkTSTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKFCDUmCgZ4xm[X[jZ3WgZZQhOTByIH7NJIFnfGW{IEK0JIhzeyCkeTDpcY12dm:kbH;0eIlv\yCvZYToc4Q> NFXmT|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4OVk1OSd-MkW2PFU6PDF:L3G+
PC3 Mo\aSpVv[3Srb36gZZN{[Xl? NGTiTXEyODBibl2= MUOxPEBpenN? MnLSTY5lfWO2aX;uJI9nKG2xbn;wc4xieiCvaYTveIlkKHOyaX7kcIV{KGmwIHj1cYFvKFCFMzDj[YxteyCjdDCxNFAhdk1iaX7jeYJifGWmIH\vdkAyQCCqcoOgZpkhcW6maYLlZ5QhcW2vdX7v[ox2d3Knc3PlcoNmKG2rY4Lvd4NweHl? NGnPS|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4OVk1OSd-MkW2PFU6PDF:L3G+
DU145 NWD3S5ZYTnWwY4Tpc44h[XO|YYm= M3fPfFExOCCwTR?= M33DNWlvcGmkaYTpc44hd2ZiUFzLNUBVcHJ{MUCgdIhwe3Cqb4L5cIF1cW:wIHnuJIh2dWGwIFTVNVQ2KGOnbHzzJIF1KDFyMDDuUUBjgSCrbX31co9jdG:2dHnu[{Bu\XSqb3S= M4PFXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nki1PVQyLz5{NU[4OVk1OTxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pro-caspase-8 / cleaved caspase-8 / cleaved caspase-3 / cleaved caspase-9; 

PubMed: 27699933     


PLKi‐induced caspase activation. HeLa cells were treated with BI 2536 (10 nmol/L), BI 6727 (68 nmol/L), or GSK461364 (11 nmol/L), and HCT 116 cells were treated with BI 2536 (20 nmol/L), BI 6727 (70 nmol/L), or GSK461364 (12 nmol/L) for 48 h. The levels of cleaved caspase (CASP)‐3, ‐8, and ‐9 were examined by Western blot analysis.

p-HH3 / PARP / Myc / p-Myc; 

PubMed: 24771642     


Western blotting showing more mitotic arrested (shown by phospho-histone H3) and apoptotic (shown by PARP) cells as well as lower MYC levels in LNCaP-Pim1 cells after BI 2536 treatment compared with LNCaP-Neo cells. The difference is obvious at lower dose of BI 2536 (10 nM). Both total MYC expression and phosphor-MYC levels were lower in Pim1 cells than Neo cells after BI 2536 treatment.

pMEK / MEK / pERK / ERK / pRSK1 / RSK1; 

PubMed: 28430578     


(A) and (B) Western blot analysis of the expression and activation of components of the MEK-ERK-RSK1 pathway in MKN45 and MKN74 cancer cells treated with BI 2536 and BI 6727 for 24 and 48 hours, respectively.

pPlk1 / Plk1 / pMyt1; 

PubMed: 28430578     


(A) and (B) Western blot analysis of the expression and phosphorylation of Plk1, and phosphorylation of Myt1 in the CSC-like and cancer cells after treatment with BI 2536 and BI 6727 for 24 and 48 hours, respectively.

Aurora B / pAurora B / pAurora C / pH3 / Cyclin B1 / Securin / pBcl-XL / MPM2; 

PubMed: 25169932     


Western blot analysis of cells treated for 24 h with the indicated inhibitor concentrations. Cell lysates were immunoblotted for Plk1, Phospho-Aurora B (T232)/Aurora C (T198), Aurora B, histone H3 phosphorylated at S10 (pH3), Cdk1, Cyclin B1, Securin, Phospho-Bcl-XL, Induced myeloid leukemia cell differentiation protein Mcl-1, MPM-2 and β-Actin. Left (Hela cells); Right (HUVEC)

27699933 24771642 28430578 25169932
Immunofluorescence
p-H2AX / propidium iodide; 

PubMed: 22309939     


Apoptotic images of the cells after BI 2536 treatment, as revealed by HCS (high-content screening) instrument (×10) with phospho-H2AX antibody or PI (propidium iodide) intake. 

Pericentrin / α-tubulin; 

PubMed: 21772266     


MEFs were labelled using Plk1, pericentrin and α-tubulin antibodies and 4,6-diamidino-2-phenylindole for the analysis by immunofluorescence microscopy. Representative examples of BI 2536-treated and control MEFs are depicted. Scale bar, 5 μm.

22309939 21772266
Growth inhibition assay
Cell viability; 

PubMed: 21772266     


Proliferative activity of MEFs at different BI 2536 concentrations (25–250 nM; means±s.d., n=3, for each concentration). *P<0.05, **P<0.01, ***P<0.001, Student's t-test, unpaired and two-tailed.

21772266
体内研究

BI 2536静脉注射给药多种移植瘤模型,每周一次或两次,效果都很好,通过使细胞周期停滞而抑制细胞增殖,随后诱导肿瘤细胞死亡。BI 2536按 50 mg/kg剂量处理HCT 116移植瘤,每周一次或两次,显著抑制肿瘤生长,T/C 分别为15%和0.3%。BI 2536每周处理两次BxPC-3和A549模型,也显著抑制肿瘤生长,T/C分别为5% 和14%。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

体外Plk1激酶实验:

重组人Plk1 (1-603残基) 作为氮端,携带杆状病毒表达系统的GST-标记的融合蛋白通过亲和层析,使用谷胱甘肽-琼脂糖而纯化。在梯度稀释的BI 2536存在时,使用 20 ng 重组激酶,及10 μg牛奶中的酪蛋白作为底物进行Plk1 酶活性实验。在终体积为60 μL 的混合物(15 mM MgCl2, 25 mM MOPS [pH 7.0], 1 mM DTT, 1% DMSO, 7.5 mM ATP, 0.3 μCi γ-33P-ATP)中30oC下进行激酶反应45分钟。加入 125 μL 冰冻的5% TCA终止反应。沉淀物转移到混合酯纤维素的多屏过滤板上,使用1% TCA冲洗实验板,然后利用放射性进行测量。绘制剂量反应曲线,而计算IC50值。
细胞实验:

[1]

- 合并
  • Cell lines: HeLa, A43, SKOV-3, HT-29, K562, A549, Saos-2, MCF7, HCT116, COLO 205, Hep G2, Raji, PC-3,等等
  • Concentrations: 溶于DMSO, 终浓度为~1 μM
  • Incubation Time: 24,和72小时
  • Method:

    使用不同浓度BI 2536处理细胞24和72小时。 在荧光分光光度计中测量Alamar Blue染料转换而测定细胞生长。为了测定培养基中DNA含量,细胞悬液在80%乙醇中混合, 使用溶于PBS的0.25% Triton X-100处理5分钟,然后与0.1% RNase 和溶于PBS的 10 μg/mL碘化丙啶在室温下温育20分钟。通过流式细胞仪分析而测定细胞周期谱。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 皮下注射HCT 116, NCI-H460, 或A549 细胞的雌性BomTac:NMRI-Foxn1nu 小鼠
  • Dosages: ~50 mg/kg
  • Administration: 静脉注射,每周一到两次
    (Only for Reference)

溶解度 (25°C)

体外 Ethanol 100 mg/mL (191.69 mM)
DMSO 21 mg/mL (40.25 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
12.5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 521.66
化学式

C28H39N7O3

CAS号 755038-02-9
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02215044 Terminated Drug: BI 2536 intravenous|Drug: Gemcitabine intravenous Pancreatic Neoplasms Boehringer Ingelheim June 2007 Phase 1
NCT02211833 Completed Drug: BI 2536|Drug: Pemetrexed Carcinoma Non-Small-Cell Lung Boehringer Ingelheim October 2006 Phase 1
NCT00701766 Completed Drug: BI 2536 Leukemia Myeloid Acute Boehringer Ingelheim October 2006 Phase 2
NCT00706498 Completed Drug: BI 2536 Prostatic Neoplasms Boehringer Ingelheim September 2006 Phase 2
NCT00376623 Completed Drug: BI 2536 Carcinoma Non-Small-Cell Lung Boehringer Ingelheim July 2006 Phase 2
NCT02211872 Completed Drug: BI 2536 BS intravenous Neoplasms Boehringer Ingelheim August 2004 Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    We need to use BI 2536 in preclinical studies using mice, how to formulate the inhibitor for this in vivo study?

  • 回答:

    For in vivo study, DMSO should not be more than 5% because the toxicity. You can formulated BI2536 in hydrochloric acid (0.1 N), and diluted with 0.9% NaCl for animal study according to the reference :( Supplemental Data) http://linkinghub.elsevier.com/retrieve/pii/S0960-9822(06)02671-6

PLK Signaling Pathway Map

PLK Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID