BI 2536

目录号:S1109

仅限科研使用

BI-2536是一种有效的Plk1抑制剂,无细胞试验中IC50为0.83 nM。BI-2536可抑制 Bromodomain 4 (BRD4) ,其Kd值为37 nM,并有效地抑制 c-Myc 的表达。BI-2536可诱导凋亡而减弱自噬。Phase 2。

BI 2536 Chemical Structure

CAS: 755038-02-9

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1978.87 现货
RMB 981.57 现货
RMB 5492.59 现货
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客户使用Selleck生产的BI 2536发表文献259篇:

产品安全说明书

PLK抑制剂选择性比较

相关PLK产品

生物活性

产品描述 BI-2536是一种有效的Plk1抑制剂,无细胞试验中IC50为0.83 nM。BI-2536可抑制 Bromodomain 4 (BRD4) ,其Kd值为37 nM,并有效地抑制 c-Myc 的表达。BI-2536可诱导凋亡而减弱自噬。Phase 2。
特性 BI 2536是第一个有效的Plk1选择性抑制剂,抑制 Plk1的标记。
靶点
c-Myc [3] PLK1 [1]
(Cell-free assay)
PLK2 [1]
(Cell-free assay)
PLK3 [1]
(Cell-free assay)
BRD4 [3]
(Cell-free assay)
0.83 nM 3.5 nM 9.0 nM 37 nM(Kd)
体外研究

除了Plk1, BI 2536也抑制 Plk2 和Plk3活性,只是抑制程度低点,IC50分别为3.5 nM和9.0 nM。BI 2536比一组63种其他蛋白激酶(IC50>10 μM)选择性高1000多倍。BI 2536 按10 nM 到 100 nM剂量作用于HeLa细胞,抑制有丝分裂中心体中γ-微管蛋白招募和Apc6 磷酸化,抑制染色体臂中释放黏结蛋白, 诱导产生单极主轴,及一些依赖Plk1的其他有丝分裂过程。与作用于Plk1 RNAi效果相一致, BI 2536处理HeLa细胞,使细胞周期停在G2/M期, 随后DNA分解及凋亡,且累积断裂的PARP p85片段,这种作用存在浓度依赖性。BI 2536抑制一组32种人肿瘤细胞系生长,EC50为2-25 nM,抑制指数生长的hTERT-RPE1,人脐静脉内皮细胞 (HUVECs),和正常大鼠肾脏(NRK) 细胞的增殖,EC50为12-31 nM。[1] BI 2536 抑制Plk1,降低甲状腺未分化癌(ATC)细胞如 CAL62, OCUT-1, SW1736, 8505C,和 ACT-1 的生长的生长和活力,EC50为1.4-5.6 nM。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PSN1 M1PETmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTBwMEC3O|Yh|ryP MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
ACN NWXFNIlyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIjt[mtKSzVyPUCuNFA5PDNizszN MmnYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
TE-8 MnPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTBwMEC5OFEh|ryP NWOxTXJGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
LC-2-ad MoPBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlrrTWM2OD1yLkCxNFk{KM7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
ALL-PO MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXWyOGYxUUN3ME2wMlAyOyEQvF2= NVjIfZJPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
EW-16 NGDLZ4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLZdVZKSzVyPUCuNFE{OzZizszN NYDMOXNwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
KM12 NGO1N4NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmf2TWM2OD1yLkCxN|Q5KM7:TR?= NIqyd2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
GI-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnWxTWM2OD1yLkCxN|g{KM7:TR?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
LS-411N NVfuN5JpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnSTWM2OD1yLkCxOFA4KM7:TR?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
ES8 Mor3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2DuOWlEPTB;MD6wNVQ6PCEQvF2= MnLlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
SNU-C2B Ml\pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1Pye2lEPTB;MD6wNVUzPiEQvF2= NWjoelY{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
CESS MljtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;jTJR4UUN3ME2wMlAyPjB{IN88US=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
697 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\XR2VzUUN3ME2wMlAyPjZ6IN88US=> NVPLbIxRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H1963 NVzWUlc6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMEG2O|Mh|ryP MkLVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
IA-LM NVX0TW9zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvXV4ZKSzVyPUCuNFE3QDhizszN MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
SW954 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUCwR3hjUUN3ME2wMlAyPzB3IN88US=> MlixQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
RL95-2 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XxSGlEPTB;MD6wNVc{QCEQvF2= NIK2VJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
LXF-289 MlziS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXnNmhKSzVyPUCuNFE4PyEQvF2= NEfkTGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
OCUB-M NWDE[o5[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1Kxe2lEPTB;MD6wNVgzPiEQvF2= NXTIeIJYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
RKO NHiyTHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWPJR|UxRTBwMEG5PFch|ryP MnPoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
LOUCY MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1z3W2lEPTB;MD6wNlA2KM7:TR?= M3H5W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NOS-1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTBwMEKwPVMh|ryP NWLBTI9vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
A4-Fuk M{POUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULY[VNEUUN3ME2wMlAzOjF6IN88US=> NGDSS3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
A388 MlXGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfjS2dEUUN3ME2wMlAzOjJ5IN88US=> M3nVcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
TE-9 M3PaUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmS0TWM2OD1yLkCyNlYyKM7:TR?= Mo\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
KURAMOCHI M1y0eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYXkUnJRUUN3ME2wMlAzOjh3IN88US=> M33jV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
KALS-1 NUTxSWxCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLqTWM2OD1yLkCyN|M5KM7:TR?= MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
HOP-62 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTBwMEK0NkDPxE1? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
RPMI-8402 NV7TU2F6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPBTWM2OD1yLkCyOVA5KM7:TR?= NFixUpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
TE-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTBwMEK1NVIh|ryP NVf0OFNIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
CCRF-CEM M4POdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDZWldKSzVyPUCuNFI2PDNizszN NE\LTZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HUTU-80 MmXLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnz1TWM2OD1yLkCyOlI1KM7:TR?= NEHyS|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1648 NISycYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3f0Z2lEPTB;MD6wNlY1PyEQvF2= M3S0VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
HCE-4 NYjBWlh1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;iZ4dKSzVyPUCuNFI3QDJizszN NWi0dGNORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
ETK-1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELRfHRKSzVyPUCuNFI4OzZizszN MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
TE-15 NX;xN3hMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTBwMEK3N|gh|ryP NYizW40{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
BE-13 MmfFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTBwMEK3OEDPxE1? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
KE-37 NXrEO2VbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\rO4JtUUN3ME2wMlAzPzRzIN88US=> NGDq[oQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1355 NVu1cm9HT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWi4W4tlUUN3ME2wMlAzPzh|IN88US=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
TE-11 MkP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLhTWM2OD1yLkCyPFA6KM7:TR?= NG[3WIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
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TE-441-T M1v3[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUL6fIFGUUN3ME2wMlUxOTJizszN M3PsXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
MDA-MB-134-VI NWLicYNlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYi2SY83UUN3ME2wMlUxQTJzIN88US=> M4C1UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
SCC-3 M336S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPtRlZKSzVyPUCuOVE5PzNizszN NInQe3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
KLE NFfvUZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTBwNUKxO|Eh|ryP MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
LB2241-RCC NXnvZ41MT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLuTWM2OD1yLkWyOVI2KM7:TR?= MoTLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NCI-H1417 NH;Ifo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDJTWM2OD1yLkWzOFM2KM7:TR?= M3HZcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H2081 NHLFeHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4L6bmlEPTB;MD61PFYyOSEQvF2= NX;WZ|BmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H1522 NEfHTYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIPLXpZKSzVyPUCuOVk{QDNizszN NEHPcY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H889 NVG5TW1RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTBwNUm4OFQh|ryP Mn7yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
CGTH-W-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4Dy[mlEPTB;MD62NFc2PSEQvF2= M3zvfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
EB-3 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3jenpnUUN3ME2wMlYyOTV|IN88US=> NYXnNZVwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
D-336MG M4CzXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFLDc5ZKSzVyPUCuOlE4QTFizszN M33qbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LC-1F M3;vbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnXqTWM2OD1yLk[yOVkzKM7:TR?= M3XkblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
MHH-NB-11 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHtZpZKSzVyPUCuOlM5ODFizszN NIfo[oM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SK-N-FI MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlu5TWM2OD1yLk[zPVEh|ryP M2\oclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H69 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DBdGlEPTB;MD62OFg4QSEQvF2= MoDHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
SCH MmjQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGjWPFJKSzVyPUCuOlYxOjNizszN MoC1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
LS-513 MoDMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTBwNkeyOVkh|ryP NICyXGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
CP67-MEL NXrjVJRsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTBwNke1PVYh|ryP NIflZYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
GCIY Mn21S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnzTWM2OD1yLkewN|Y{KM7:TR?= NGr3SZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SHP-77 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoriTWM2OD1yLkewO|M1KM7:TR?= NEjIPHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
EHEB M3m1OGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnMTWM2OD1yLkexPFU1KM7:TR?= NIXr[JQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
D-247MG NVzaeoI5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTBwN{OyN|Ih|ryP M3u2OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
Raji NIn5e3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nOVmlEPTB;MD63OFk5QSEQvF2= NHn2PJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
MPP-89 NVPWZZNWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPEVZhKSzVyPUCuO|U6PzhizszN MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
EKVX MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\oUoF6UUN3ME2wMlc6ODd|IN88US=> MoLZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
HCC2218 MmrRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HLOGlEPTB;MD63PVE{OSEQvF2= NILvZYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
KNS-81-FD NIS1cG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV6zWol5UUN3ME2wMlgyODFzIN88US=> M4DZd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
HCC2157 NVzoPGZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TIUGlEPTB;MD64OFk2OSEQvF2= NV;vWZc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
BT-474 M1jGTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTBwOEi4OVQh|ryP NXLYPW91RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H748 M{PpVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUflcZRSUUN3ME2wMlkyOiEQvF2= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
EW-24 NXTpPFI4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\QTGlEPTB;MD65NlAxOiEQvF2= MkW0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
D-263MG MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MknWTWM2OD1yLkm0OVEzKM7:TR?= M4fJNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
OMC-1 M2TLOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTBwOU[zO|Mh|ryP MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H2196 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MknwTWM2OD1yLkm3O|c4KM7:TR?= MkX6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
HAL-01 MnrsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\DT5FKSzVyPUCuPVk5PzJizszN MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H2107 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITlZ5JKSzVyPUGuNFE5PTRizszN M{PT[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LB996-RCC M3vPcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTFwMEiwOVEh|ryP MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H1395 M3PseGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTFwMUC2O{DPxE1? NGPEO3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
CHP-126 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDKW4tTUUN3ME2xMlE3OTl7IN88US=> NHTJ[ZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NB69 M1LNNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3P1emlEPTB;MT6yOlE1QCEQvF2= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
CAS-1 M4qzXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHwXm1RUUN3ME2xMlM1OjNzIN88US=> NYXD[JRxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
JVM-3 MnLLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NE\xbXRKSzVyPUGuOFc5PTVizszN Ml65QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
DMS-153 Mm\DS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MorxTWM2OD1zLkW3Olc1KM7:TR?= MlSwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
SK-MEL-2 NWnTWIpnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljNTWM2OD1zLkexO|g6KM7:TR?= NUPqXW1jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H1155 M3e2fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHyUVVRUUN3ME2xMlc{Pjd4IN88US=> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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LU-165 NEnEXYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDkTWM2OD1zLki2OFc3KM7:TR?= NEDIVVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
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C4-2 MkDLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33tRWlEPTB;ODDuUS=> Mn3XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3MEWxO|QoRjJ3NUC1NVc1RC:jPh?=
JURKAT NIDNSnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnyR3AyOjRiaB?= M{jsZmlEPTB;NEG2MlY3KG6P Mne0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
MOLT-4 Mmq1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;xbpVHOjRiaB?= NUnUN5BtUUN3ME2yPFU{NjVibl2= M33wZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
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RS 4;11 M2DF[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXmyOEBp MlPETWM2OD13MUmuNFUhdk1? MlnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
697 NHrVT2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;yNlQhcA>? MmLuTWM2OD1{MUC3MlEhdk1? NFLnbJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
SIL-ALL M1XQbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYKyOEBp M17yPGlEPTB;MUm5Mlc4KG6P NFnZe449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
CEM MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVP6Z|JwOjRiaB?= M3HWZ2lEPTB;NUKuNVghdk1? M3\xdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
P12 NXn4NmJqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XCeVI1KGh? M1zze2lEPTB;M{muNlchdk1? M3jzUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
NALM-16 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XxOFI1KGh? M{C0U2lEPTB;OU[uNlghdk1? Mkj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
JURKAT Ml;xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\4OFghcA>? M4HyfWlEPTB;MUSuPFQhdk1? M4nn[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
MOLT-4 NGTMcWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX60PEBp MkfYTWM2OD1{Nj62JI5O NHf4[2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
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NALM-6 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfkcVloPDhiaB?= M3y5T2lEPTB;MkOuNlYhdk1? M1vmRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
RS 4;11 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjvOFghcA>? NFTadVFKSzVyPUG2OE4zQCCwTR?= NUXURo9RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
697 NF\6OmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXy0PEBp MV;JR|UxRTNyNT6zJI5O NGD3NVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
SIL-ALL NVrTNo9YT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFOxWWQ1QCCq MXjJR|UxRTd2Lk[yJI5O Mn3UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
CEM NG\XWFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\hNHU1QCCq NHTXeIFKSzVyPUK0MlU2KG6P MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
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NALM-16 NHHZcm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mon3OFghcA>? MVLJR|UxRTZ|LkK0JI5O MkHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
JURKAT Mn;PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\2WlczKGh? M2\EeWlEPTB;Mz62OUBvVQ>? MlHRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
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RS 4;11 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXW3NkBp NUPjR|ByUUN3ME20MlM5KG6P Moi1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
697 NUfyZ5ZsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV63NkBp Mm[5TWM2OD15ND6yPEBvVQ>? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
SIL-ALL NWnlXWJNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7YZ20{PzJiaB?= MnLyTWM2OD15LkKgcm0> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
CEM MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1z1eVczKGh? MkfLTWM2OD13LkSyJI5O M2LGZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
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NALM-16 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVzWOYFmPzJiaB?= M2\PXGlEPTB;N{euO|Mhdk1? NYTPV2Q3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
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Assay
Methods Test Index PMID
Western blot pro-caspase-8 / cleaved caspase-8 / cleaved caspase-3 / cleaved caspase-9 p-HH3 / PARP / Myc / p-Myc pMEK / MEK / pERK / ERK / pRSK1 / RSK1 pPlk1 / Plk1 / pMyt1 Aurora B / pAurora B / pAurora C / pH3 / Cyclin B1 / Securin / pBcl-XL / MPM2 27699933 24771642 28430578 25169932
Immunofluorescence p-H2AX / propidium iodide Pericentrin / α-tubulin 22309939 21772266
Growth inhibition assay Cell viability 21772266
体内研究

BI 2536静脉注射给药多种移植瘤模型,每周一次或两次,效果都很好,通过使细胞周期停滞而抑制细胞增殖,随后诱导肿瘤细胞死亡。BI 2536按 50 mg/kg剂量处理HCT 116移植瘤,每周一次或两次,显著抑制肿瘤生长,T/C 分别为15%和0.3%。BI 2536每周处理两次BxPC-3和A549模型,也显著抑制肿瘤生长,T/C分别为5% 和14%。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

  • 体外Plk1激酶实验:

    重组人Plk1 (1-603残基) 作为氮端,携带杆状病毒表达系统的GST-标记的融合蛋白通过亲和层析,使用谷胱甘肽-琼脂糖而纯化。在梯度稀释的BI 2536存在时,使用 20 ng 重组激酶,及10 μg牛奶中的酪蛋白作为底物进行Plk1 酶活性实验。在终体积为60 μL 的混合物(15 mM MgCl2, 25 mM MOPS [pH 7.0], 1 mM DTT, 1% DMSO, 7.5 mM ATP, 0.3 μCi γ-33P-ATP)中30oC下进行激酶反应45分钟。加入 125 μL 冰冻的5% TCA终止反应。沉淀物转移到混合酯纤维素的多屏过滤板上,使用1% TCA冲洗实验板,然后利用放射性进行测量。绘制剂量反应曲线,而计算IC50值。

细胞实验:

[1]

  • Cell lines: HeLa, A43, SKOV-3, HT-29, K562, A549, Saos-2, MCF7, HCT116, COLO 205, Hep G2, Raji, PC-3,等等
  • Concentrations: 溶于DMSO, 终浓度为~1 μM
  • Incubation Time: 24,和72小时
  • Method:

    使用不同浓度BI 2536处理细胞24和72小时。 在荧光分光光度计中测量Alamar Blue染料转换而测定细胞生长。为了测定培养基中DNA含量,细胞悬液在80%乙醇中混合, 使用溶于PBS的0.25% Triton X-100处理5分钟,然后与0.1% RNase 和溶于PBS的 10 μg/mL碘化丙啶在室温下温育20分钟。通过流式细胞仪分析而测定细胞周期谱。

动物实验:

[1]

  • Animal Models: 皮下注射HCT 116, NCI-H460, 或A549 细胞的雌性BomTac:NMRI-Foxn1nu 小鼠
  • Dosages: ~50 mg/kg
  • Administration: 静脉注射,每周一到两次

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O

12.5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 521.66
化学式

C28H39N7O3

CAS号 755038-02-9
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02215044 Terminated Drug: BI 2536 intravenous|Drug: Gemcitabine intravenous Pancreatic Neoplasms Boehringer Ingelheim June 2007 Phase 1
NCT02211833 Completed Drug: BI 2536|Drug: Pemetrexed Carcinoma Non-Small-Cell Lung Boehringer Ingelheim October 2006 Phase 1
NCT00701766 Completed Drug: BI 2536 Leukemia Myeloid Acute Boehringer Ingelheim October 2006 Phase 2
NCT00706498 Completed Drug: BI 2536 Prostatic Neoplasms Boehringer Ingelheim September 2006 Phase 2
NCT00376623 Completed Drug: BI 2536 Carcinoma Non-Small-Cell Lung Boehringer Ingelheim July 2006 Phase 2
NCT02211872 Completed Drug: BI 2536 BS intravenous Neoplasms Boehringer Ingelheim August 2004 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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常见问题及建议解决方法

问题 1:
We need to use BI 2536 in preclinical studies using mice, how to formulate the inhibitor for this in vivo study?

回答:
For in vivo study, DMSO should not be more than 5% because the toxicity. You can formulated BI2536 in hydrochloric acid (0.1 N), and diluted with 0.9% NaCl for animal study according to the reference :( Supplemental Data) http://linkinghub.elsevier.com/retrieve/pii/S0960-9822(06)02671-6