BI 2536

For research use only. Not for use in humans.

目录号:S1109

BI 2536 Chemical Structure

CAS No. 755038-02-9

BI-2536是一种有效的Plk1抑制剂,无细胞试验中IC50为0.83 nM。BI-2536可抑制 Bromodomain 4 (BRD4) ,其Kd值为37 nM,并有效地抑制 c-Myc 的表达。BI-2536可诱导凋亡而减弱自噬。Phase 2。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1978.87 现货
RMB 981.57 现货
RMB 5492.59 现货
RMB 8160.83 现货
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客户使用Selleck生产的BI 2536发表文献257篇:

产品安全说明书

PLK抑制剂选择性比较

生物活性

产品描述 BI-2536是一种有效的Plk1抑制剂,无细胞试验中IC50为0.83 nM。BI-2536可抑制 Bromodomain 4 (BRD4) ,其Kd值为37 nM,并有效地抑制 c-Myc 的表达。BI-2536可诱导凋亡而减弱自噬。Phase 2。
特性 BI 2536是第一个有效的Plk1选择性抑制剂,抑制 Plk1的标记。
靶点
c-Myc [3]
()
PLK1 [1]
(Cell-free assay)
PLK2 [1]
(Cell-free assay)
PLK3 [1]
(Cell-free assay)
BRD4 [3]
(Cell-free assay)
0.83 nM 3.5 nM 9.0 nM 37 nM(Kd)
体外研究

除了Plk1, BI 2536也抑制 Plk2 和Plk3活性,只是抑制程度低点,IC50分别为3.5 nM和9.0 nM。BI 2536比一组63种其他蛋白激酶(IC50>10 μM)选择性高1000多倍。BI 2536 按10 nM 到 100 nM剂量作用于HeLa细胞,抑制有丝分裂中心体中γ-微管蛋白招募和Apc6 磷酸化,抑制染色体臂中释放黏结蛋白, 诱导产生单极主轴,及一些依赖Plk1的其他有丝分裂过程。与作用于Plk1 RNAi效果相一致, BI 2536处理HeLa细胞,使细胞周期停在G2/M期, 随后DNA分解及凋亡,且累积断裂的PARP p85片段,这种作用存在浓度依赖性。BI 2536抑制一组32种人肿瘤细胞系生长,EC50为2-25 nM,抑制指数生长的hTERT-RPE1,人脐静脉内皮细胞 (HUVECs),和正常大鼠肾脏(NRK) 细胞的增殖,EC50为12-31 nM。[1] BI 2536 抑制Plk1,降低甲状腺未分化癌(ATC)细胞如 CAL62, OCUT-1, SW1736, 8505C,和 ACT-1 的生长的生长和活力,EC50为1.4-5.6 nM。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PSN1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn76TWM2OD1yLkCwO|c3KM7:TR?= MlqyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
ACN NW\re5FYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MY\JR|UxRTBwMEC4OFMh|ryP NWrTRWtERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
TE-8 M4\5N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXzJR|UxRTBwMEC5OFEh|ryP NInTTFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
LC-2-ad NGrl[JBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTBwMEGwPVMh|ryP NVu4UFdyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
ALL-PO NFq1b|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfMOlhwUUN3ME2wMlAyOyEQvF2= M2fZXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
EW-16 M1fiT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTBwMEGzN|Yh|ryP MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
KM12 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEfoXJZKSzVyPUCuNFE{PDhizszN MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
GI-1 NIjyXllIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVTK[ZN5UUN3ME2wMlAyOzh|IN88US=> MoHtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
LS-411N MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTBwMEG0NFch|ryP NUnVOoFsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
ES8 NVXrSVU1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LyUGlEPTB;MD6wNVQ6PCEQvF2= NF3Qb4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SNU-C2B MmnpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{ezNWlEPTB;MD6wNVUzPiEQvF2= NV3DV3F4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
CESS NV\NT2ZnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{H5dWlEPTB;MD6wNVYxOiEQvF2= NFTNd249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
697 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17RNGlEPTB;MD6wNVY3QCEQvF2= NHz3dHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1963 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV65cW1qUUN3ME2wMlAyPjd|IN88US=> NH3PcIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
IA-LM NFi5W3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjYcI9qUUN3ME2wMlAyPjh6IN88US=> MoXEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
SW954 NXnuTIkyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnuwTWM2OD1yLkCxO|A2KM7:TR?= NVjNNXRORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
RL95-2 MknrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HPemlEPTB;MD6wNVc{QCEQvF2= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
LXF-289 M4LmR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTBwMEG3O{DPxE1? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
OCUB-M NHLhdYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Moj1TWM2OD1yLkCxPFI3KM7:TR?= NFm5VJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
RKO M2fEOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TOZWlEPTB;MD6wNVk5PyEQvF2= M4jCNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LOUCY NX7jcGtuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTLRnNTUUN3ME2wMlAzODVizszN NHfmSFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NOS-1 NUXD[pNLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHO0[m5KSzVyPUCuNFIxQTNizszN MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
A4-Fuk NEDUbZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XJZmlEPTB;MD6wNlIyQCEQvF2= MlK4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
A388 M2DPU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml3uTWM2OD1yLkCyNlI4KM7:TR?= MkjRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
TE-9 NUezeGdET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4HxfmlEPTB;MD6wNlI3OSEQvF2= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
KURAMOCHI MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGThdlBKSzVyPUCuNFIzQDVizszN M1TuWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
KALS-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHBZZhKSzVyPUCuNFI{OzhizszN M2jOe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
HOP-62 NGizWW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jaWWlEPTB;MD6wNlQzKM7:TR?= Mn63QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
RPMI-8402 NIj4cGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWe5dlQ6UUN3ME2wMlAzPTB6IN88US=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
TE-1 M4nOZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1rXWmlEPTB;MD6wNlUyOiEQvF2= MmHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
CCRF-CEM NVLMOI5sT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWm3c5c2UUN3ME2wMlAzPTR|IN88US=> NIDZNIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HUTU-80 NXvzVmVrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2n6fGlEPTB;MD6wNlYzPCEQvF2= NHqzNI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1648 NHvVd3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfjXW5KUUN3ME2wMlAzPjR5IN88US=> NVrz[FhVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
HCE-4 M4exXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLyTWM2OD1yLkCyOlgzKM7:TR?= M4fwZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
ETK-1 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Moj6TWM2OD1yLkCyO|M3KM7:TR?= NEXFd3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
TE-15 M4PoWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrGcGI3UUN3ME2wMlAzPzN6IN88US=> MoXmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
BE-13 NHe3NI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXNTWM2OD1yLkCyO|Qh|ryP NF;xZo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
KE-37 M2DaWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIGxb5VKSzVyPUCuNFI4PDFizszN NELsVHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1355 M4Lw[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYLJR|UxRTBwMEK3PFMh|ryP NHzLOHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
TE-11 M1LMeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\VS5NKSzVyPUCuNFI5ODlizszN Mk\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
BL-70 M{TlSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MomxTWM2OD1yLkCyPFU4KM7:TR?= NGjqO3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
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LB2241-RCC NWrGSnVmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPtTWM2OD1yLkWyOVI2KM7:TR?= NUnUfnNORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H1417 MmHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HIRWlEPTB;MD61N|Q{PSEQvF2= NIXHc4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H2081 MkDDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWm4dnFwUUN3ME2wMlU5PjFzIN88US=> MmL5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NCI-H1522 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTjb3NCUUN3ME2wMlU6Ozh|IN88US=> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H889 M13YN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUD2NVZbUUN3ME2wMlU6QDR2IN88US=> M4XDRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
CGTH-W-1 NF7LWmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjmOIVKSzVyPUCuOlA4PTVizszN NWLqT|NHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
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D-336MG MlPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVHUd4xPUUN3ME2wMlYyPzlzIN88US=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
LC-1F MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;QTWM2OD1yLk[yOVkzKM7:TR?= MlnYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
MHH-NB-11 MonuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2SzOWlEPTB;MD62N|gxOSEQvF2= M1PXTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
SK-N-FI MkfMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX33ZVE3UUN3ME2wMlY{QTFizszN M1rMRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H69 NXL4TGxrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4n0cmlEPTB;MD62OFg4QSEQvF2= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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LS-513 MnXTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\0NmlFUUN3ME2wMlY4OjV7IN88US=> NGLsZWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
CP67-MEL NHjFNphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\vTWM2OD1yLk[3OVk3KM7:TR?= M1\ldVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
GCIY M320dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoDiTWM2OD1yLkewN|Y{KM7:TR?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
SHP-77 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vVdWlEPTB;MD63NFc{PCEQvF2= NX;tTppyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
EHEB MoDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jlZWlEPTB;MD63NVg2PCEQvF2= NUjoUnRtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
D-247MG MlnxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2r5RWlEPTB;MD63N|I{OiEQvF2= M{jyU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
Raji M32xN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;iTWM2OD1yLke0PVg6KM7:TR?= NVv1WJl5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
MPP-89 NGD2U2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmGyTWM2OD1yLke1PVc5KM7:TR?= NUfwO4FMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
EKVX MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPGRohKSzVyPUCuO|kxPzNizszN NFHFdJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HCC2218 NHPPRnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTBwN{mxN|Eh|ryP MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
KNS-81-FD M1zjT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlPlTWM2OD1yLkixNFEyKM7:TR?= NHjGbJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HCC2157 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXTufW9sUUN3ME2wMlg1QTVzIN88US=> NVPSfVBxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
BT-474 NFjJPHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTBwOEi4OVQh|ryP NGjZSFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H748 NIfrXY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXTHV5B[UUN3ME2wMlkyOiEQvF2= NUDyemg1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
EW-24 M3vLNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2DlRmlEPTB;MD65NlAxOiEQvF2= NX;HcVl4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
D-263MG NEjGTnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJR|UxRTBwOUS1NVIh|ryP NX7vN21iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
OMC-1 Mn\QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml\zTWM2OD1yLkm2N|c{KM7:TR?= NXHZSWV5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H2196 NF;O[IVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoTNTWM2OD1yLkm3O|c4KM7:TR?= Mn;MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
HAL-01 NEDMVodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3fmdmlEPTB;MD65PVg4OiEQvF2= M3:0Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H2107 NHPEfWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nXWGlEPTB;MT6wNVg2PCEQvF2= M324fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LB996-RCC Ml[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTFwMEiwOVEh|ryP NXi3PY96RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H1395 M1rLT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;yTWM2OD1zLkGwOlch|ryP MmLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
CHP-126 NWC4TXl3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkPETWM2OD1zLkG2NVk6KM7:TR?= M4HxVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NB69 NYnWeYd6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\yOGlEPTB;MT6yOlE1QCEQvF2= MmPaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
CAS-1 NHPWWmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nuT2lEPTB;MT6zOFI{OSEQvF2= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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DMS-153 Moj6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4Pze2lEPTB;MT61O|Y4PCEQvF2= NFP2SVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SK-MEL-2 M3TqZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\OZ2lEPTB;MT63NVc5QSEQvF2= MoPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NCI-H1155 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxRTFwN{O2O|Yh|ryP MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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MSTO-211H MlnYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITOXIRKSzVyPUKxMlg5PThizszN NV3xdZhMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
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NH-12 M2nQXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XrT2lEPTB;M{OuNlAyPyEQvF2= M4nEfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
COLO-824 Mof1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXXTItbUUN3ME2zOE4yODB4IN88US=> NVTaTpNNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
SKM-1 NIGxW2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmXVTWM2OD1|Nz64OFIzKM7:TR?= NH3oR2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1838 MkTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn[1TWM2OD1|OD6zO|k4KM7:TR?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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KG-1 MlTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoWzTWM2OD12MD64PVU2KM7:TR?= MmTZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
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LNCaP-Clone-FGC NYG4coR[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn3ETWM2OD12MT65NFE4KM7:TR?= Mn3tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
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C4-2 MmmzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;1UGZKSzVyPUigcm0> NF7vRVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWwOVE4PCd-MkW1NFUyPzR:L3G+
JURKAT M{G5Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fHPVI1KGh? NXPw[3l5UUN3ME20NVYvPjZibl2= M3mwVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
MOLT-4 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;hTFI1KGh? MULJR|UxRTJ6NUOuOUBvVQ>? M4j2UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
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NALM-6 NIS0TlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrJ[llmOjRiaB?= NEnD[WZKSzVyPUO0N|MxKG6P MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
RS 4;11 NYPMdVlbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVeyOEBp M4f4WGlEPTB;NUG5MlA2KG6P M2X0TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
697 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY[yOEBp NYLKZ5ZGUUN3ME2yNVA4NjFibl2= NX70UppjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
SIL-ALL MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;zNlQhcA>? MkT3TWM2OD1zOUmuO|chdk1? M{LSNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
CEM NIHuRoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nOc|I1KGh? NUL6XZoyUUN3ME21Nk4yQCCwTR?= M3XPU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
P12 M4HDO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{WxbVI1KGh? MnKxTWM2OD1|OT6yO{BvVQ>? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
NALM-16 NFz2ZYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmH4NlQhcA>? MVjJR|UxRTl4LkK4JI5O MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
JURKAT MmfkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvmbVQ1QCCq NV[5UYlzUUN3ME2xOE45PCCwTR?= M1i0XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
MOLT-4 NGfTTZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{fINlQ5KGh? M2DOSWlEPTB;Mk[uOkBvVQ>? NGf1NFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
REH MoCwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1yzU|Q5KGh? NF61VJFKSzVyPUS0MlcyKG6P MkL3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
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RS 4;11 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jKeFQ5KGh? M2jR[GlEPTB;MU[0MlI5KG6P MnjrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
697 NUnFWW15T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmPCOFghcA>? MkWyTWM2OD1|MEWuN{BvVQ>? NFroWmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
SIL-ALL NHjVUJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnntOFghcA>? MUnJR|UxRTd2Lk[yJI5O M4XaO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
CEM Mn:1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXe0PEBp MYTJR|UxRTJ2LkW1JI5O MnLSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
P12 M4nIfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnPHOFghcA>? M{K3ZWlEPTB;MUiuOFYhdk1? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
NALM-16 NHX6cnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7lOFghcA>? NXvDbnFEUUN3ME22N{4zPCCwTR?= Mnz6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
JURKAT MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zySFczKGh? NH7adJVKSzVyPUOuOlUhdk1? NUTsXXdIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
MOLT-4 MkjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\USYw4OiCq MX;JR|UxRTdwM{mgcm0> Mk\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
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NALM-6 NGjMSHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TlflczKGh? NVPueXZkUUN3ME2xMlc5KG6P MmO0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
RS 4;11 NIe3RY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4nRWlczKGh? NFzZW4RKSzVyPUSuN|ghdk1? NYexS2d5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
697 M134UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rIeVczKGh? MnTwTWM2OD15ND6yPEBvVQ>? NYPx[W9jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
SIL-ALL M2mxVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGS5Tm84OiCq MnLtTWM2OD15LkKgcm0> NHWzepM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
CEM M{DMZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWrzVIdHPzJiaB?= MnPZTWM2OD13LkSyJI5O M3PkclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
P12 M{\3Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\kO|IhcA>? NUXFRVVnUUN3ME22MlM4KG6P NF[yVo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
NALM-16 M2PCR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLpO|IhcA>? NWPLS4NpUUN3ME23O{44OyCwTR?= NHe0Smg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
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WER-Rb-1 MknaRZBweHSxc3nzJGF{e2G7 NYrRN2c3OS13MDDuUS=> M4X1eFEzNzJ2IHi= NFnSTopFVVOR NEDrfYtqdmS3Y3XkJIFxd3C2b4Ppd{B4cGmlaDDjZY4h[mViZX7oZY5k\WRiYomgTHQ> M13Qe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{[2OlY2Lz5{NEO2OlY3PTxxYU6=
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SJ-GBM2 NV\hXYcxeUiWUzDhd5NigQ>? NY\ac3NQeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= NVPDXphMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 NUDRW4hieUiWUzDhd5NigQ>? M3jVSZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> MnrIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh18 Mnn6dWhVWyCjc4PhfS=> Mm\ldWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVogyQCClZXzsdy=> NUnR[ZF3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 NXzWWWp7eUiWUzDhd5NigQ>? NFyzU2lyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBU[W:|LUKgZ4VtdHN? NYHoco9XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
DU145 MVrBdI9xfG:|aYOgZZN{[Xl? MUixNFAhdk1? M4Luc|I1KGi{cx?= MnfRTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCGVUG0OUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCSQWLQJINt\WG4YXflJIF1KDFyMDDuUUBi\nSncjCyOEBpenNiYomgbY1ufW6xYnzveJRqdmdibXX0bI9l NU\RO2JnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFU6PDFpPkK1Olg2QTRzPD;hQi=>
HeLa NEDaWnpHfW6ldHnvckBie3OjeR?= M{\CTlExOCCwTR?= NIn5fpdKdmirYnn0bY9vKG:oIGDMT|EhXGi{MkGwJJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDI[WxiKGOnbHzzJIF1KDFyMDDuUUBjgSCrbX31co9jdG:2dHnu[{Bu\XSqb3S= MnTXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4OEW5OFEoRjJ3Nki1PVQyRC:jPh?=
DU145 Moj6SpVv[3Srb36gZZN{[Xl? M3e3cFExOCCwTR?= MlvpTY5pcWKrdHnvckBw\iCSTFuxJJBzd3SnaX6gZYNkfW23bHH0bY9vKGmwIHj1cYFvKESXMUS1JINmdGy|IHH0JFExOCCwTTDifUBqdW23bn;icI91fGmwZzDt[ZRpd2R? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NUm0NUc,OjV4OEW5OFE9N2F-
HeLa MoPVSpVv[3Srb36gZZN{[Xl? MX6xNFAhdk1? MVnJcohq[mm2aX;uJI9nKFCOS{GgdJJwfGWrbjDhZ4N2dXWuYYTpc44hcW5iaIXtZY4hUGWOYTDj[YxteyCjdDCxNFAhdk1iYomgbY1ufW6xYnzveJRqdmdibXX0bI9l M2LwbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nki1PVQyLz5{NU[4OVk1OTxxYU6=
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HeLa Mk\CRZBweHSxc3nzJIF{e2G7 MmfhNVAxKG6P MlzYNlQhcHK| MkTuTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKFCDUmCgZ4xm[X[jZ3WgZZQhOTByIH7NJIFnfGW{IEK0JIhzeyCkeTDpcY12dm:kbH;0eIlv\yCvZYToc4Q> M3vUclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nki1PVQyLz5{NU[4OVk1OTxxYU6=
PC3 MWjGeY5kfGmxbjDhd5NigQ>? M{jM[VExOCCwTR?= Mk\nNVghcHK| NHLzWnlKdmS3Y4Tpc44hd2ZibX;uc5BwdGG{IH3peI91cWNic4DpcoRt\XNiaX6gbJVu[W5iUFOzJINmdGy|IHH0JFExOCCwTTDpcoN2[mG2ZXSg[o9zKDF6IHjyd{BjgSCrbnTpdoVkfCCrbX31co9ndHWxcnXzZ4Vv[2VibXnjdo9{[2:yeR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NUm0NUc,OjV4OEW5OFE9N2F-
DU145 MUTGeY5kfGmxbjDhd5NigQ>? NVf3fFhwOTByIH7N NW\oW4VUUW6qaXLpeIlwdiCxZjDQUGsyKFSqckKxNEBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gSHUyPDViY3XscJMh[XRiMUCwJI5OKGK7IHntcZVvd2Kub4T0bY5oKG2ndHjv[C=> NV\iPI1lRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFU6PDFpPkK1Olg2QTRzPD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pro-caspase-8 / cleaved caspase-8 / cleaved caspase-3 / cleaved caspase-9; 

PubMed: 27699933     


PLKi‐induced caspase activation. HeLa cells were treated with BI 2536 (10 nmol/L), BI 6727 (68 nmol/L), or GSK461364 (11 nmol/L), and HCT 116 cells were treated with BI 2536 (20 nmol/L), BI 6727 (70 nmol/L), or GSK461364 (12 nmol/L) for 48 h. The levels of cleaved caspase (CASP)‐3, ‐8, and ‐9 were examined by Western blot analysis.

p-HH3 / PARP / Myc / p-Myc; 

PubMed: 24771642     


Western blotting showing more mitotic arrested (shown by phospho-histone H3) and apoptotic (shown by PARP) cells as well as lower MYC levels in LNCaP-Pim1 cells after BI 2536 treatment compared with LNCaP-Neo cells. The difference is obvious at lower dose of BI 2536 (10 nM). Both total MYC expression and phosphor-MYC levels were lower in Pim1 cells than Neo cells after BI 2536 treatment.

pMEK / MEK / pERK / ERK / pRSK1 / RSK1; 

PubMed: 28430578     


(A) and (B) Western blot analysis of the expression and activation of components of the MEK-ERK-RSK1 pathway in MKN45 and MKN74 cancer cells treated with BI 2536 and BI 6727 for 24 and 48 hours, respectively.

pPlk1 / Plk1 / pMyt1; 

PubMed: 28430578     


(A) and (B) Western blot analysis of the expression and phosphorylation of Plk1, and phosphorylation of Myt1 in the CSC-like and cancer cells after treatment with BI 2536 and BI 6727 for 24 and 48 hours, respectively.

Aurora B / pAurora B / pAurora C / pH3 / Cyclin B1 / Securin / pBcl-XL / MPM2; 

PubMed: 25169932     


Western blot analysis of cells treated for 24 h with the indicated inhibitor concentrations. Cell lysates were immunoblotted for Plk1, Phospho-Aurora B (T232)/Aurora C (T198), Aurora B, histone H3 phosphorylated at S10 (pH3), Cdk1, Cyclin B1, Securin, Phospho-Bcl-XL, Induced myeloid leukemia cell differentiation protein Mcl-1, MPM-2 and β-Actin. Left (Hela cells); Right (HUVEC)

27699933 24771642 28430578 25169932
Immunofluorescence
p-H2AX / propidium iodide; 

PubMed: 22309939     


Apoptotic images of the cells after BI 2536 treatment, as revealed by HCS (high-content screening) instrument (×10) with phospho-H2AX antibody or PI (propidium iodide) intake. 

Pericentrin / α-tubulin; 

PubMed: 21772266     


MEFs were labelled using Plk1, pericentrin and α-tubulin antibodies and 4,6-diamidino-2-phenylindole for the analysis by immunofluorescence microscopy. Representative examples of BI 2536-treated and control MEFs are depicted. Scale bar, 5 μm.

22309939 21772266
Growth inhibition assay
Cell viability; 

PubMed: 21772266     


Proliferative activity of MEFs at different BI 2536 concentrations (25–250 nM; means±s.d., n=3, for each concentration). *P<0.05, **P<0.01, ***P<0.001, Student's t-test, unpaired and two-tailed.

21772266
体内研究

BI 2536静脉注射给药多种移植瘤模型,每周一次或两次,效果都很好,通过使细胞周期停滞而抑制细胞增殖,随后诱导肿瘤细胞死亡。BI 2536按 50 mg/kg剂量处理HCT 116移植瘤,每周一次或两次,显著抑制肿瘤生长,T/C 分别为15%和0.3%。BI 2536每周处理两次BxPC-3和A549模型,也显著抑制肿瘤生长,T/C分别为5% 和14%。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

体外Plk1激酶实验:

重组人Plk1 (1-603残基) 作为氮端,携带杆状病毒表达系统的GST-标记的融合蛋白通过亲和层析,使用谷胱甘肽-琼脂糖而纯化。在梯度稀释的BI 2536存在时,使用 20 ng 重组激酶,及10 μg牛奶中的酪蛋白作为底物进行Plk1 酶活性实验。在终体积为60 μL 的混合物(15 mM MgCl2, 25 mM MOPS [pH 7.0], 1 mM DTT, 1% DMSO, 7.5 mM ATP, 0.3 μCi γ-33P-ATP)中30oC下进行激酶反应45分钟。加入 125 μL 冰冻的5% TCA终止反应。沉淀物转移到混合酯纤维素的多屏过滤板上,使用1% TCA冲洗实验板,然后利用放射性进行测量。绘制剂量反应曲线,而计算IC50值。
细胞实验:

[1]

- 合并
  • Cell lines: HeLa, A43, SKOV-3, HT-29, K562, A549, Saos-2, MCF7, HCT116, COLO 205, Hep G2, Raji, PC-3,等等
  • Concentrations: 溶于DMSO, 终浓度为~1 μM
  • Incubation Time: 24,和72小时
  • Method:

    使用不同浓度BI 2536处理细胞24和72小时。 在荧光分光光度计中测量Alamar Blue染料转换而测定细胞生长。为了测定培养基中DNA含量,细胞悬液在80%乙醇中混合, 使用溶于PBS的0.25% Triton X-100处理5分钟,然后与0.1% RNase 和溶于PBS的 10 μg/mL碘化丙啶在室温下温育20分钟。通过流式细胞仪分析而测定细胞周期谱。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 皮下注射HCT 116, NCI-H460, 或A549 细胞的雌性BomTac:NMRI-Foxn1nu 小鼠
  • Dosages: ~50 mg/kg
  • Administration: 静脉注射,每周一到两次
    (Only for Reference)

溶解度 (25°C)

体外 Ethanol 100 mg/mL (191.69 mM)
DMSO 21 mg/mL (40.25 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
12.5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 521.66
化学式

C28H39N7O3

CAS号 755038-02-9
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02215044 Terminated Drug: BI 2536 intravenous|Drug: Gemcitabine intravenous Pancreatic Neoplasms Boehringer Ingelheim June 2007 Phase 1
NCT02211833 Completed Drug: BI 2536|Drug: Pemetrexed Carcinoma Non-Small-Cell Lung Boehringer Ingelheim October 2006 Phase 1
NCT00701766 Completed Drug: BI 2536 Leukemia Myeloid Acute Boehringer Ingelheim October 2006 Phase 2
NCT00706498 Completed Drug: BI 2536 Prostatic Neoplasms Boehringer Ingelheim September 2006 Phase 2
NCT00376623 Completed Drug: BI 2536 Carcinoma Non-Small-Cell Lung Boehringer Ingelheim July 2006 Phase 2
NCT02211872 Completed Drug: BI 2536 BS intravenous Neoplasms Boehringer Ingelheim August 2004 Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    We need to use BI 2536 in preclinical studies using mice, how to formulate the inhibitor for this in vivo study?

  • 回答:

    For in vivo study, DMSO should not be more than 5% because the toxicity. You can formulated BI2536 in hydrochloric acid (0.1 N), and diluted with 0.9% NaCl for animal study according to the reference :( Supplemental Data) http://linkinghub.elsevier.com/retrieve/pii/S0960-9822(06)02671-6

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID