Bortezomib (PS-341)

目录号:S1013 别名: Velcade, LDP-341, MLM341, NSC 681239 中文名称:硼替佐米

仅限科研使用

Bortezomib (PS-341, Velcade, LDP-341, MLM341, NSC 681239)是有效的蛋白酶抑制剂,Ki为0.6 nM。它对肿瘤细胞表现出良好的选择性。Bortezomib (PS-341) 可抑制 NF-κB 并诱导 ERK 的磷酸化从而抑制cathepsin B并在卵巢癌和其他固体肿瘤中抑制自噬的催化过程。

Bortezomib (PS-341) Chemical Structure

CAS: 179324-69-7

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 749.39 现货
RMB 571.94 现货
RMB 2188.92 现货
RMB 5488.32 现货
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客户使用Selleck生产的Bortezomib (PS-341)发表文献845篇:

产品安全说明书

Proteasome抑制剂选择性比较

相关Proteasome产品

生物活性

产品描述 Bortezomib (PS-341, Velcade, LDP-341, MLM341, NSC 681239)是有效的蛋白酶抑制剂,Ki为0.6 nM。它对肿瘤细胞表现出良好的选择性。Bortezomib (PS-341) 可抑制 NF-κB 并诱导 ERK 的磷酸化从而抑制cathepsin B并在卵巢癌和其他固体肿瘤中抑制自噬的催化过程。
靶点
NF-κB [6] 20S proteasome [1]
(Cell-free assay)
0.6 nM(Ki)
体外研究

Bortezomib,一种硼酸二肽,是一种26S蛋白酶体的高选择性可逆抑制剂,其作用于错误折叠蛋白的降解,并且对细胞周期的调控是必要的。暴露于Bortezomib能够稳定p21,p27,和p53,以及促凋亡Bid和Bax蛋白,微囊蛋白-1,和抑制剂κB-α,这防止了核因子κB诱导的细胞存活途径的激活。Bortezomib也会促进促凋亡c-Jun-NH2末端激酶,以及内质网应激反应的激活。这些细胞蛋白水平的改变导致对增殖,迁移的抑制,和癌细胞凋亡的促进。[2] Bortezomib能够渗透到细胞,并抑制蛋白酶体介导的细胞内长寿蛋白水解,抑制50%蛋白质水解的浓度为∼0.1 μM。Bortezomib对衍生自美国国家癌症研究所(NCI)多重人类肿瘤的一组60个癌细胞系的IC50值为7 nM。PC-3细胞用Bortezomib (100 nM)处理8小时导致细胞积聚在G2-M期,相应的G1期细胞数量减少。Bortezomib在24和48小时杀死PC-3细胞,IC50分别为100和20 nM。Bortezomib治疗16-24小时后诱导细胞核缩合。Bortezomib在低至100 nM浓度下以时间依赖的方式导致PARP裂解,在处理24小时后产生效果。[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H1838 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYf4N|h4UUN3ME20NVg3NjN{IN88US=> M2\CPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
IMR-5 NWPrbJd1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTN2MEmuOlIh|ryP NWjnVnFwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
TE-441-T M2TnVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjzWGh6UUN3ME2yOVIyNjdizszN M{DIbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
U-698-M NUTEVZY6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTJ{NkKuNVUh|ryP NEfm[lE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
COLO-824 M4jkdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfTTIhKSzVyPUGyOlEvPzhizszN MlHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
P31-FUJ NVvLT|RoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLmVmhKSzVyPUGxNVIvPzVizszN MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlUxPDFxJ{7TRW5ITVJ:L3G+
KY821 NXHxflJXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTFyM{[uNFQh|ryP NHXMcHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
RPMI-8866 NFjVc|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7MbWdKSzVyPUGwNFYvOjhizszN Ml\RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
TC-YIK NHG2NXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWfJR|UxRTd6MT6wNUBvVQ>? NYPGZZo1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
MS-1 NWW1ZpJCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVPoZ441UUN3ME23OVkvPDJibl2= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlUxPDFxJ{7TRW5ITVJ:L3G+
DMS-153 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYPZenpJUUN3ME23OFYvQDNibl2= MlTpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
SUP-T1 NH\sb2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHvTWM2OD14OE[uNFQhdk1? NFPBW209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
SCC-15 M{fGV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH;0VIlKSzVyPU[2O{41PyCwTR?= M1XsdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
MSTO-211H NIfzUFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYDJR|UxRTV5ND6yOkBvVQ>? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlUxPDFxJ{7TRW5ITVJ:L3G+
J-RT3-T3-5 M4izbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\x[pRVUUN3ME21N|IvPTdibl2= NUT2dpF[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
NCI-H889 NELXXWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXTTWM2OD12NkGuPVIhdk1? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlUxPDFxJ{7TRW5ITVJ:L3G+
CPC-N NUjDT5lqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV3JR|UxRTRyMz63O{BvVQ>? NF2yemE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
COLO-668 NVLUNmw5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrYO3pnUUN3ME20NFMvPTdibl2= NIqwV2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
NCI-H226 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mln1TWM2OD12MEOuNlMhdk1? MnfUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
TUR MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4exOGlEPTB;M{m2MlYyKG6P MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlUxPDFxJ{7TRW5ITVJ:L3G+
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NCI-H1963 NXizdnY2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHrTWM2OD1|OE[uNVkhdk1? MnX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
CA46 MnXmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofBTWM2OD1|N{OuOlMhdk1? NWTxdo5JRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
SNU-C1 M1HmXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUHJR|UxRTN4Mj6wPUBvVQ>? NYrPZ4JORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
THP-1 M{TISWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYjJR|UxRTN{Mj62JI5O NFrVV4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
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NCI-H1522 MkD0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXDTWM2OD1|MEeuNFUhdk1? MnztQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
LNCaP-Clone-FGC MoTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fxdWlEPTB;Mkm1MlI3KG6P NVXzNWczRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
NCI-H2171 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3H3[GlEPTB;Mki4MlkzKG6P NFS3eXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
NCI-H187 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\qZW56UUN3ME2yPFcvODhibl2= Mk\MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
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NCI-H1155 MojyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTJ|MD6zNkBvVQ>? Mn30QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
NCI-H1395 MnPRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEWxW|dKSzVyPUKxNE4yOyCwTR?= NXy2fVZvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
KM-H2 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\MTWM2OD1zOUeuNFUhdk1? Mn\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
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L-540 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLIUGJKSzVyPUGuN|chdk1? NHqydm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
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DU-4475 NF\sbGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIryNnlKSzVyPUGuN|Yhdk1? MmTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
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Daudi NX:xfnhlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUfJR|UxRTFwMkKgcm0> M3TpOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
NCI-H1882 NV\IV2s2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4rpb2lEPTB;MT6yJI5O MonTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
TE-8 NFzxR|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXTJR|UxRTFwMUmgcm0> NEjxfW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
HAL-01 M1j2ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUO1eFlIUUN3ME2xMlE5KG6P NWL2UZhTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
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ACN NX3kT3NUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWTJR|UxRTBwNEG3JI5O NH3Be5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
D-247MG NEPYUHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUGwfGlsUUN3ME2wMlQyOyCwTR?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlUxPDFxJ{7TRW5ITVJ:L3G+
HCC2998 M3qxZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3TUXGlEPTB;MD60NVIhdk1? NVvlOlFwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
MZ2-MEL NGj2V45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLZTWM2OD1yLkSwO{BvVQ>? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlUxPDFxJ{7TRW5ITVJ:L3G+
ES8 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\6dnN[UUN3ME2wMlQhdk1? MmjoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
A388 M4fm[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{LwfWlEPTB;MD6zOVYhdk1? MlrEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
KS-1 NX7uWnhyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVXJR|UxRTBwM{Sgcm0> M{jrN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
BB30-HNC M3q1PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPERWxmUUN3ME2wMlM{PSCwTR?= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlUxPDFxJ{7TRW5ITVJ:L3G+
ONS-76 M1\ic2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn\sTWM2OD1yLkOzJI5O NITDV|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
D-542MG Mn;CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rBNGlEPTB;MD6zNlkhdk1? M2XaNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
BB65-RCC NEmzSm9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHrbnFQUUN3ME2wMlMxPCCwTR?= MnOyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
LOUCY NH;6dYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XZfmlEPTB;MD6yPVMhdk1? M1K2NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
OVCAR-4 MmOzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTBwMki5JI5O M2rueFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
LXF-289 Ml7OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;5WmFyUUN3ME2wMlI3QSCwTR?= NHK5e4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
KNS-42 M33vOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHXzOIpKSzVyPUCuNlU5KG6P NIrYc|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
8-MG-BA NEjHdXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTBwMkWgcm0> M1nydFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
NTERA-S-cl-D1 NXnMSYxkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPaTWM2OD1yLkK0N{BvVQ>? M4qweFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFMzPTB2MT:nQnNCVkeHUkyvZV4>
A101D MkLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nabWlEPTB;MD6yNlUhdk1? NXTRUnA5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
MMAC-SF NF7YUlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\lTHpVUUN3ME2wMlIyPiCwTR?= NV\pRlRLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
no-10 MmDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfsRXJ[UUN3ME2wMlIyKG6P MlvwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
A253 MnLQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjtTWM2OD1yLkKwPEBvVQ>? MnryQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
TE-9 MkLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PS[WlEPTB;MD6xPFIhdk1? NV22NmR5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOzJ3MESxM{c,W0GQR1XSQE9iRg>?
SH-4 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJR|UxRTBwMUezJI5O MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNlUxPDFxJ{7TRW5ITVJ:L3G+
SK-UT-1 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonuTWM2OD1yLkG2N{BvVQ>? Ml60QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMN|I2ODRzLze+V2FPT0WUPD;hQi=>
ES6 M2PVTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3;UPGlEPTB;MD6wNFIyKG6P NHPHRow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw{OjVyNEGvK|5USU6JRWK8M4E,
human PBMC M1jmUWZ2dmO2aX;uJGF{e2G7 NH3vT4EyODBibl2= MWKyOEBp M1;j[Glv\HWlZYOgTWwuQCC{ZXzlZZNm NUDtNo5XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3PVE1PzdpPkK1O|kyPDd5PD;hQi=>
U937 M1jEcmZ2dmO2aX;uJGF{e2G7 MYCxNFAhdk1? NX;CXZJuPiCq NITNUINKdmS3Y3XzJGlNNThiZYjwdoV{e2mxbjDpckBNWFNvc4TpcZVt[XSnZDDVPVM4KG2jY4LvdIhi\2W| MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd7MUS3O{c,OjV5OUG0O|c9N2F-
HH M1zhNW1q\3KjdHnvckBCe3OjeR?= NE\sRWUyODBibl2= MlPKNlQhcA>? NYHTbIZMTE2VTx?= NFHQ[ZNT\WS3Y3XzJINmdGxibXnndoF1cW:wIHL5JFgx6oDVOUGl NGLkbGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4NVM{PSd-MkW2PFE{OzV:L3G+
Hut-78 Mk[xUYloemG2aX;uJGF{e2G7 NYTUUW9lOTByIH7N NYXDfmpCOjRiaB?= MYDEUXNQ M3LkNXJm\HWlZYOgZ4VtdCCvaXfyZZRqd25iYomgPFDjiJN7MDW= M3fVdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkixN|M2Lz5{NU[4NVM{PTxxYU6=
HH MUXGeY5kfGmxbjDBd5NigQ>? MXKxNFAhdk1? M3H3WlI1KGh? Mn[0SG1UVw>? M{XyXoRwf26{ZXf1cIF1\XNiVFfGMe6zOSCjbnSgTWwuOTJiZYjwdoV{e2mxbh?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6MUOzOUc,OjV4OEGzN|U9N2F-
H9 NW\Sd2FpTnWwY4Tpc44hSXO|YYm= M1PQOFExOCCwTR?= MX:yOEBp NFLJNZhFVVOR M1rTeGRwf26{ZXf1cIF1\XNiVFfGMe6zOSCjbnSgTWwuOTFiZYjwdoV{e2mxbh?= NGTjN4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4NVM{PSd-MkW2PFE{OzV:L3G+
Hut-78 M4DRc2Z2dmO2aX;uJGF{e2G7 MX2xNFAhdk1? M1jlclI1KGh? M2HPfWROW09? NHHvSndFd3ewcnXneYxifGW|IGTHSk3PujFiYX7kJGlNNTFyIHX4dJJme3Orb36= NEL4N3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4NVM{PSd-MkW2PFE{OzV:L3G+
H1299 M2HhXmFxd3C2b4Ppd{BCe3OjeR?= NWPON|lYQDBibl2= M3G3clI1KGh? MYfEUXNQ MoLvV4Vve2m2aYrld{BPW0OOQzDj[YxteyC2bzDNV2Mu\GW{aY\l[EBqSzlvaX7keYNm\CCjcH;weI9{cXN? M4jxbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{KzOlk{Lz5{NUOyN|Y6OzxxYU6=
BLM NWKxfJZkSXW2b4DoZYd6KEG|c3H5 NYPSVZloOTBibl2= NVfLUnBzOTJiaB?= NIq3VGRKdmS3Y3XzJIZwem2jdHnvckBw\iCjdYTvdIhi\2:|b33ldy=> M2S0[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEe5NFg{Lz5{M{C3PVA5OzxxYU6=
A375 M2DTb2F2fG:yaHHnfUBCe3OjeR?= NWP5Umx1OTBibl2= MkHONVIhcA>? NYC0dWtTUW6mdXPld{Bnd3KvYYTpc44hd2ZiYYX0c5Bp[Wexc3;t[ZM> MnrQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyN{mwPFMoRjJ|MEe5NFg{RC:jPh?=
BLM NG\zbYxCeG:ydH;zbZMhSXO|YYm= M1vCSFExKG6P Ml3aNlQhcA>? NIi5bnJKdmS3Y3XzJINmdGxiYYDvdJRwe2m| NWrQSmQzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwO|kxQDNpPkKzNFc6ODh|PD;hQi=>
A375 NH7PPVNCeG:ydH;zbZMhSXO|YYm= M1rZO|ExKG6P Mk\ONlQhcA>? MV7JcoR2[2W|IHPlcIwh[XCxcITvd4l{ NH\5O5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C3PVA5Oyd-MkOwO|kxQDN:L3G+
RAW 264.7 NFL0OpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;1PXR2OTByIH7N MmLyOFghcA>? NXq5[JFrWmWmdXPld{Bk\WyuII\pZYJqdGm2edMg MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{N{G1OEc,OjJ2MkexOVQ9N2F-
SNK-6 MYXBcpRqfmm{YXygRZN{[Xl? Mn60NUDPxE1? NH;2PJczPCCq MWnJcoR2[2W|IHz5eIlkKGmwZnXjeIlwdiCxZjDFRnY> MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF5MEm4PEc,OjFzN{C5PFg9N2F-
KAI-3 NVvQN4luSW62aY\pdoFtKEG|c3H5 M2DoWlEh|ryP M1jTd|I1KGh? NWHhb5NbUW6mdXPld{BtgXSrYzDpcoZm[3Srb36gc4YhTUKY MmK1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzN{C5PFgoRjJzMUewPVg5RC:jPh?=
SNT-16 MmrhRY51cX[rcnHsJGF{e2G7 MX[xJO69VQ>? NI\abIkzPCCq MVfJcoR2[2W|IHz5eIlkKGmwZnXjeIlwdiCxZjDFRnY> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF5MEm4PEc,OjFzN{C5PFg9N2F-
SNT-13 MVXBcpRqfmm{YXygRZN{[Xl? MnT2NUDPxE1? NGLzRYczPCCq NFvMVoZKdmS3Y3XzJIx6fGmlIHnu[oVkfGmxbjDv[kBGSlZ? NITNd4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUG3NFk5QCd-MkGxO|A6QDh:L3G+
KHYG-1 MmTURZBweHSxc3nzJGF{e2G7 NXL4eJA4OSEQvF2= NEnEcmY3KGh? NXO3S3J{UW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF5MEm4PEc,OjFzN{C5PFg9N2F-
KAI-3 MXfBdI9xfG:|aYOgRZN{[Xl? M3Hp[|Eh|ryP NVjo[FJIPiCq NHLvOGFKdmS3Y3XzJINmdGxiYYDvdJRwe2m| NEKyclM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUG3NFk5QCd-MkGxO|A6QDh:L3G+
Jurkat NIXtRllCeG:ydH;zbZMhSXO|YYm= MVmxJO69VQ>? MlfrOkBp MX7JcoR2[2W|IHPlcIwh[XCxcITvd4l{ MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF5MEm4PEc,OjFzN{C5PFg9N2F-
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Glioma (HF2790) NIrwdnNEgXSxdH;4bYNqfHliYYPzZZk> MoPtO|IhcHK| MnvtSWM2OCB;IECuNFAxPyEQvF2= NUHselVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yPFg3QTlpPkK2Nlg5Pjl7PD;hQi=>
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GUMBUS M3vnWGN6fG:2b4jpZ4l1gSCjc4PhfS=> M1fQOlczKGi{cx?= M3;6PGlEPTBiPTCwMlAxPiEQvF2= M3XvUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MEOxOVkxLz5{M{CzNVU6ODxxYU6=
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RPMI8226 MWrBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NXv4R|JNPzJiaILz NXfub5dqUUN3MDC9JFAvODB4NzFOwG0> NUPYTFVHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkixPFI6QTBpPkK4NVgzQTlyPD;hQi=>
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HL60 MkTQR5l1d3SxeHnjbZR6KGG|c3H5 MnrQO|IhcHK| NGnwOJNKSzVyIE2gNE4xODdzIN88US=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF3OEG4OEc,OjBzNUixPFQ9N2F-
MKN45 MX;BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NFXuPFg4OiCqcoO= MWnJR|UxKD1iMD6wNFcyPSEQvF2= NGO2RmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{e2PVA{Oyd-Mke3OlkxOzN:L3G+
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RPMI8226 NILTdFBEgXSxdH;4bYMh[WO2aY\peJkh[WejaX7zeEBpfW2jbh?= Mmm1O|IhcHK| M{f2U2lEPTBiPTCwMlAxPzl4IN88US=> NFL1NY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2O|gyQCd-MkS3Olc5OTh:L3G+
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U266 MmGzR5l1d3SxeHnjbZR6KGG|c3H5 NGLZZXE4OiCqcoO= NF;qVGJKSzVyIE2gNE4xODh6IN88US=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF3OEG4OEc,OjBzNUixPFQ9N2F-
RPMI8226 NVPDbXdYS3m2b4TvfIlkcXS7IHHzd4F6 M4fTXmlEPTBiPTCwMlAxQDhizszN NIry[mo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEeyO|c1Pid-MkC3Nlc4PDZ:L3G+
RPMI8226 MnzrR5l1d3SxeHnjbZR6KGG|c3H5 MWC0PEB1dyB5MjDodpM> MnfVTWM2OCB;IECuNFA6PSEQvF2= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFzOUW1PUc,OjRzMUm1OVk9N2F-
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HCT116 MX3DfZRwfG:6aXPpeJkh[XO|YYm= NImx[Ig4OiCqcoO= MmnoTWM2OCB;IECuNFEh|ryP NG\5eVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W0O|c2Pyd-MkO1OFc4PTd:L3G+
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CCRF-CEM NHKyeFFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MnKyO|IhcHK| M2TpW2lEPTBiPTCwMlAyOSEQvF2= M{KwOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MkOxNVYzLz5{NkKzNVE3OjxxYU6=
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MDA-MB-231 MXnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M3LkOVczKGi{cx?= M4\2XWlEPTBiPTCwMlAyPjZ3IN88US=> MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzd4OUCzN{c,Ojd5NkmwN|M9N2F-
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fibroblast NFTKOXVyUFSVIHHzd4F6 M2LuSZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiClb370do9tKEiqIIfpcIQhfHmyZTDmbYJzd2KuYYP0JINmdGy| NFPEfZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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A673 MnLGdWhVWyCjc4PhfS=> MljPdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgRVY4OyClZXzsd{k> MnXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 NVzmfFdGeUiWUzDhd5NigQ>? M1nn[pFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTN5IHPlcIx{ M2nyc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
MG 63 (6-TG R) MonodWhVWyCjc4PhfS=> M1TGbJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG1IKDZ|IDi2MXRIKFJrIHPlcIx{ NX:wVndnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh30 NVy3fpRleUiWUzDhd5NigQ>? MkOxdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog{OCClZXzsdy=> NY\OepdDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
fibroblast M4nnU5FJXFNiYYPzZZk> NVOxWHIzeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5Ih[2:wdILvcEBJcCC5aXzkJJR6eGViZnnido9jdGG|dDDj[Yxtew>? NHO2V2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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OHS-50 NXzDdW9TeUiWUzDhd5NigQ>? NVjzN|d6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| NUjlXld[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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Assay
Methods Test Index PMID
Western blot phospho-PERK / ATF-4 GRP-78 / GADD-34 pro-caspase-12 / pro-caspase-3 pro-caspase-9 p-IκBα / IκBα COX-2 / cIAP2 15509775 20335171 24085292
Immunofluorescence Vimentin Ubiquitin 19010849
Growth inhibition assay Cell viability 20571067
ELISA IL-8 24085292
体内研究

Bortezomib单一用药的抗癌作用已在多发性骨髓瘤的异种移植模型,成人白血病,肺癌,乳腺癌,前列腺癌,胰腺癌,头颈癌,和结肠癌,以及在黑色素瘤中得到证实。[2]在Lewis肺癌模型中,口服bortezomib (1.0 mg/ kg,每天),服用18天引起肿瘤生长延迟,并减少转移数量。Bortezomib单一用药, 高达5 mg/kg剂量时显著降低乳腺癌细胞的存活率。在前列腺癌小鼠异种移植模型中,Bortezomib (1.0 mg/kg,每周一次)用药4周减少60%肿瘤生长。1.0 mg/kg Bortezomib给药4周导致胰腺癌小鼠异种移植物生长减少72%或84%,并导致肿瘤细胞凋亡增加。1.0 mg/kg Bortezomib显著抑制人浆细胞瘤异种移植物生长,增加肿瘤细胞凋亡和总存活率,并减少肿瘤血管生成。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[4]

  • 动力学法:

    在典型的动力学试验中,2.00 mL试验缓冲液(20 mM HEPES,0.5 mM EDTA,0.035% SDS,pH 7.8) 和 Suc-Leu-Leu-Val-Tyr-AMC溶于DMSO,加入3 mL荧光比色皿中,并将比色皿放置于荧光分光光度计的夹套细胞皿座。反应温度通过循环水浴维持在37℃。反应溶液达到热平衡后(5 分钟),1 μL−10 μL储存酶溶液加入培养皿。伴随AMC 从多肽AMC底物裂解的反应进程通过440 nm (λex= 380 nm)下荧光发射的增加监测。

细胞实验:

[5]

  • Cell lines: 人多发性骨髓瘤细胞系U266
  • Concentrations: ~10 μM
  • Incubation Time: 2天
  • Method:

    通过测定细胞吸收 MTT染料的情况而测定 Bortezomib对 MM和 BMSC 生长的抑制情况。每孔使用10 μL 5 mg/mL MTT对培养48小时的细胞进行脉冲处理,至少处理4小时, 随后加入100 μL 含0.04 N HCl的异丙醇 。使用分光光度计在 570 nm处测定吸光值。

动物实验:

[3]

  • Animal Models: 人浆细胞瘤异种移植物RPMI 8226
  • Dosages: 1mg/kg
  • Administration: i.v.,一周两次,使用4周,然后一周一次

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+ddH2O

5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 384.24
化学式

C19H25BN4O4

CAS号 179324-69-7
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04656951 Recruiting Drug: Daratumumab Multiple Myeloma University of Cologne|Janssen-Cilag G.m.b.H June 1 2021 Phase 2
NCT04915248 Active not recruiting Drug: Daratumumab|Drug: Bortezomib|Drug: Dexamethasone Plasmablastic Lymphoma Fondazione Italiana Linfomi ONLUS|Janssen-Cilag S.p.A. March 18 2021 Phase 2
NCT04570631 Recruiting Drug: Eftozanermin alfa|Drug: Bortezomib|Drug: Dexamethasone Multiple Myeloma AbbVie November 5 2020 Phase 1
NCT03993262 Recruiting Drug: Bortezomib|Drug: Placebo Autoimmune Encephalitis Jena University Hospital May 13 2020 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

技术支持

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操作手册

如果有其他问题,请给我们留言。

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常见问题及建议解决方法

问题 1:
On your website, it is mentioned that Bortezomib should be prepared at a concentration of 5 mg/ml in 2% DMSO/30% PEG300/ddH2O for in vivo use. But on the product sheet we received with the compound, it is mentioned: 5mg/ml in 0.5% methylcellulose, 0.2% tween 80. So which is the correct preparation buffer?

回答:
S1013 Bortezomib in 2% DMSO+30% PEG 300+ddH2O at 5 mg/ml is a clear solution, and it in 0.5% methylcellulose+0.2% Tween 80 is a suspension. Please choose the suitable vehicle according to your administration route. When you prepare the clear solution, please dissolve Bortezomib in DMSO first, make sure it dissolves well, warm it up to 45 degree and/or sonicate if necessary, then add PEG, mix well, and finally add water.