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S3I-201

目录号：S1155 别名: NSC 74859

仅限科研使用

S3I-201 (NSC 74859) 有效抑制STAT3的DNA结合活性，IC50为86 μM，而对STAT1和STAT5的抑制活性低。

CAS: 501919-59-1

规格	价格	库存
10mM (1mL in DMSO)	RMB 638.93	现货
5mg	RMB 563.02	现货
10mg	RMB 972.36	现货
50mg	RMB 3028.28	现货
大包装	有超大折扣

今日订购，明日送达全国免运费分装免费分装成小规格产品（单支最小为1mg）如1支10mg产品可分装为10支1mg产品全国免费电话：400-668-6834 \| Email：info@selleck.cn

客户使用Selleck生产的S3I-201发表文献197篇：

产品安全说明书

STAT抑制剂选择性比较

生物活性

产品描述

S3I-201 (NSC 74859) 有效抑制STAT3的DNA结合活性，IC50为86 μM，而对STAT1和STAT5的抑制活性低。

特性

S3I-201是化学探测器抑制剂，可以除去乳腺瘤。

靶点

STAT3 ^[1]
(Cell-free assay)

86 μM

体外研究

S3I-201选择性抑制Stat3 DNA结合活性。S3I-201抑制Stat3·Stat3复合形式,不依赖于Stat3的激活状态。S3I-201不干涉Lck SH2与同源pTyr肽结合。S3I-201作用于NIH 3T3/v-Src鼠成纤维细胞和人类乳腺癌MDA-MB-231, MDA-MB-435, 和MDA-MB-468 细胞，可以抑制Stat3激活。S3I-201 抑制Stat3依赖的转录活性。 S3I-201 也抑制编码cyclin D1, Bcl-xL,和surviving 的Stat3-调节基因的表达。^[1]S3I-201可以降低 pS727STAT3水平和降低TGF-β通路蛋白水平。S3I-201也可抑制CD133+ 和CD133− Huh-7细胞。^[2]最新研究显示S3I-201作用于Hep-G2, Huh-7和SK-HEP-1细胞，加强 Cetuximab的抗增殖效果。^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

U87	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M2Lld\|czKGh?		M3rTUWlEPTB;NUWuNUDPxE1?	Ml3aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByN{K2OVIoRjJyMEeyOlUzRC:jPh?=
U373	MnHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		Mn\XO\|IhcA>?		MnnuTWM2OD13Mj61JO69VQ>?	NGnaUoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC3NlY2Oid-MkCwO\|I3PTJ:L3G+
HPAC	NHHqTVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MknoO\|IhcA>?		NX7KVWRMUUN3MP-8olExOCEQvF2=	M3;O[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEeyOlUzLz5{MEC3NlY2OjxxYU6=
PANC-1	M{HzXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NInxTZg4OiCq		MX3JR\|Ux97zgMUCwJO69VQ>?	M4fRfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEeyOlUzLz5{MEC3NlY2OjxxYU6=
SK-BR-3	NUXpfm1OT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MnLEO\|IhcA>?		M{n3Z2lEPTExvK6xNFAh\|ryP	NWfqOo1sRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwO\|I3PTJpPkKwNFczPjV{PD;hQi=>
U-373 MG	NUL1bml3S3m2b4TvfIlkcXS7IFHzd4F6	MnO5N{8yOCEQvF2=	MX2yOEBp		MWfy[YR2[2W\|IF\OMe6{NWmwZIXj[YQh[2WubDDu[ZVzd3SxeHnjbZR6	NWXsRYx6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4PFg1OTZpPkKwPFg5PDF4PD;hQi=>
MDA-MB-231	NF72[HJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		M2nLV\|czKGh?		M2rSR2lEPTExvK6xNFAh\|ryP	NH;NN3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC3NlY2Oid-MkCwO\|I3PTJ:L3G+
HUVEC	NH2z[INHfW6ldHnvckBCe3OjeR?=	NHjEPXkxNjVvMkCg{txO	MYOyOEBp	Mm\ESG1UVw>?	NHHBSld{fXCycnXzd4V{KHSqZTDofZBwgGmjLXnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEiLRj2x{tE>	M{LFS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUKzOVU6Lz5{MUWyN\|U2QTxxYU6=
Huh7	M1nCZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmXTNVAxKG6P	MVW0PEBp	NWjJeFZ6TE2VTx?=	Ml6wbY5pcWKrdIOgeIhmKEmOLU[gd5RqdXWuYYTpc44heHKxbX;0[YQh[2WubDDwdo9tcW[ncnH0bY9v	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN4NEO4PUc,OjN\|NkSzPFk9N2F-
PLC/PRF/5	NGfp[FRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1\LS\|ExOCCwTR?=	MkLYOFghcA>?	M{TxXWROW09?	MXnpcohq[mm2czD0bIUhUUxvNjDzeIlufWyjdHnvckBxem:vb4Tl[EBk\WyuIIDyc4xq\mW{YYTpc44>	MnvuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN\|NkSzPFkoRjJ\|M{[0N\|g6RC:jPh?=
H460	M3f4V2Z2dmO2aX;uJGF{e2G7	MXG1NE8yODBizszN	NEHpZXY1QCCq		NGrq[5BqdmirYnn0d{B1cGViU4TheFNEKGmwY4LlZZNm\CCvaWKtPVJiKGW6cILld5Nqd25?	NH\o[YU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyNFI2PCd-MkO4NlAzPTR:L3G+
H1299	M2TsUWZ2dmO2aX;uJGF{e2G7	NIniVG82OC9zMECg{txO	M12zWlQ5KGh?		Mo\hd5VxeHKnc4Pld{BucVJvOULhJIV5eHKnc4Ppc44h\G:\|ZT3k[ZBmdmSnboTsfS=>	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{MEK1OEc,OjN6MkCyOVQ9N2F-
T-cell	NU\iPWM6T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				Mn;CTWM2OD13MDFOwG0>	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB4OEezNUc,OjRyNki3N\|E9N2F-
U373	Mln6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYqxNlUh\|ryP	NWf2S2xMOjRiaB?=	MmX0SG1UVw>?	MmXX[Il{enWydIOgV3RCXDNic3nncoFtcW6pIHHu[EBxem:uaX\ldoF1cW:w	M3z0elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEewPFIxLz5{NEC3NFgzODxxYU6=
HUT-102	NWDSV2ZwSXCxcITvd4l{KEG\|c3H5	Mn;DO\|UuOzByIN88US=>	M{TRelI1NzR6IHi=		NXn6Zpdne3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25iaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJiYX7kJIlv\HWlZYOgZ4VtdCCjcH;weI9{cXQEoB?=	MmHlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUC5PVUoRjJ2MEmwPVk2RC:jPh?=
MT-2	NV6xcGNqSXCxcITvd4l{KEG\|c3H5	NH3MOW44PS1\|MECg{txO	MVKyOE81QCCq		NHjlWWZ{fXCycnXzd4V{KGOnbHygdJJwdGmoZYLheIlwdiCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meiCjbnSgbY5lfWOnczDj[YxtKGGyb4D0c5Nqe8Li	NFy5eYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5NFk6PSd-MkSwPVA6QTV:L3G+
H460	NX6zR29CSXCxcITvd4l{KEG\|c3H5	M2HKcVExOMLibl2=	NWDV[IFXOjUEoHi=		NEnqXYhmdmijbnPld{Bk\WyuIHTlZZRpKGOxLYTy[YF1\WRid3n0bEBNYTJ7NECwNi=>	NVOzOFA2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O\|I2OzhpPkK0OFczPTN6PD;hQi=>
A459	MXfBdI9xfG:\|aYOgRZN{[Xl?	MUexNFDDqG6P	M4jZZlI1yqCq		M1\HNYlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3:teJJm[XSnZDD3bZRpKEKHWkKzOS=>	MlHsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{K1N\|goRjJ2NEeyOVM5RC:jPh?=
H460	NVL6Zm9{SXCxcITvd4l{KEG\|c3H5	M1PyVlExOMLibl2=	NH7EZlYzPMLiaB?=		M3Pyeolv\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3:teJJm[XSnZDD3bZRpKEKHWkKzOS=>	M3\nRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEeyOVM5Lz5{NES3NlU{QDxxYU6=
GC	M1;Tdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M121ZlUxNTF{NTFOwG0>	M4LxVFczKGh?		NVr0bFRF[XS2ZX71ZZRmeyC2aHWgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI>	M1vJTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{e0OVA{Lz5{NUe3OFUxOzxxYU6=
GH3	NYPSbmZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MofDOVAuOTJ3IN88US=>	NFLL[VU4OiCq		MXjheJRmdnWjdHXzJJRp\SClZXzsJIdzd3e2aDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?=	NEnrWHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe3OFUxOyd-MkW3O\|Q2ODN:L3G+
BT474R	M3niSWZ2dmO2aX;uJGF{e2G7	M3e4XlUxKM7:TR?=	M1LsTFExNTZyIHS=		NXPFVYh6cW6qaXLpeJMhW1SDVEOgZYN1cX[rdIm=	NH7oO209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOyO\|U3OSd-MkWzNlc2PjF:L3G+
NCI-N87R	NGf1WFBHfW6ldHnvckBCe3OjeR?=	MnHBOVAh\|ryP	MmfGNVAuPjBiZB?=		MmGzbY5pcWKrdIOgV3RCXDNiYXP0bZZqfHl?	M4[wWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{K3OVYyLz5{NUOyO\|U3OTxxYU6=
MDA-MB-468	M1y1NGZ2dmO2aX;uJIF{e2G7	NFjoWWYyODBidV2=	M2r1R\|I1KGi{cx?=		MY\Jcohq[mm2aX;uJI9nKFO2YYSzJIFkfGm4YYTpc44hcW5iaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK\|	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-435	MmC0SpVv[3Srb36gZZN{[Xl?	MWCxNFAhfU1?	NF;GZnIzPCCqcoO=		MUTJcohq[mm2aX;uJI9nKFO2YYSzJIFkfGm4YYTpc44hcW5iaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK\|	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-231	M1HBfWZ2dmO2aX;uJIF{e2G7	NWjYb45{OTByIIXN	NVm1[Zh4OjRiaILz		MWnJcohq[mm2aX;uJI9nKFO2YYSzJIFkfGm4YYTpc44hcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK\|	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
NIH3T3	NYPQWJV5TnWwY4Tpc44h[XO\|YYm=	NG\vblMyODBidV2=	MoD1NlQhcHK\|		M4DT[3Jm\HWldHnvckBw\iCyVInyMVcxPSCVdHH0N{Bt\X[nbDDpckB3NVO{YzDlfJBz\XO\|aX7nJI1wfXOnIF7JTFNVOyClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK\|	MlnRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
NIH3T3	MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?	NF6zWFYyODBidV2=	MUW0JIRigXN?		MUnHdo94fGhiaX7obYJqfGmxbjDv[kBud3W\|ZTDOTWg{XDNiY3XscJMh\XiycnXzd4lv\yC4LWPyZ{BifCBzMECgeW0h[W[2ZYKgOEBl[Xm\|IHL5JJRzgXCjbjDicJVmKGW6Y3z1d4lwdiCjc4PhfS=>	M1W1U\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N\|A6ODxxYU6=
MDA-MB-435	NFrIXHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	NU\DN\|VxOTByIIXN	NUn4PZZDPCCmYYnz		MYrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNSGEuVUJvNEO1JINmdGy\|IHX4dJJme3Orbnegek1UemNiYYSgNVAxKHWPIHHmeIVzKDRiZHH5d{BjgSC2conwZY4h[my3ZTDlfINtfXOrb36gZZN{[Xl?	NYKxSWdSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
MDA-MB-231	M1m1PWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	NEX3W\|YyODBidV2=	MWe0JIRigXN?		NVX4R5RiT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BmgHC{ZYPzbY5oKHZvU4LjJIF1KDFyMDD1UUBi\nSncjC0JIRigXNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG\|c3H5	NF2xdYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N\|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-468	Mlu1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?	MYSxNFAhfU1?	Ml\rOEBl[Xm\|		NX;3NpVXT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{BmgHC{ZYPzbY5oKHZvU4LjJIF1KDFyMDD1UUBi\nSncjC0JIRigXNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG\|c3H5	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
NIH3T3	M1fMWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7	MVGxNFAhfU1?			NF3ab\|BIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBPUUh\|VEOgZ4VtdHNiZYjwdoV{e2mwZzD2MXJieyCjdDCxNFAhfU1iZn;yJIV3\XK7IEOg[IF6eyCkeTDzc4Z1NWGpYYKgZ49td267LX\vdo1ifGmxbjDhd5NigQ>?	NELjOpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N\|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-435	NEXnbmJCeG:ydH;zbZMh[XO\|YYm=	MkPUN\|AhfG9iMUCwJJVO	MUS0PEBpenN?		MonDTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCPRFGtUWIuPDN3IHPlcIx{KGW6cILld5NqdmdiYXP0bZZmKFO2YYSzJIF1KDNyIITvJFExOCC3TTDh[pRmeiB2ODDodpM>	NHu2cXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N\|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-231	NVmyUIN1SXCxcITvd4l{KGG\|c3H5	M1HjVlExOCC3TR?=	NH;JWpkzPCCqcoO=		MWXS[YR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIGP0ZZQ{KHS{YX7z[oVkfGWmIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiMUCwJJVOKGGodHXyJFI1KGi{cx?=	NFi5ZXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N\|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-231	NH7Vc3NHfW6ldHnvckBie3OjeR?=	NIfie5cyODBidV2=	NH3jZ3g1QCCqcoO=		MmnkVoVlfWO2aX;uJI9nKGO7Y3zpckBFOSCpZX7lJIV5eHKnc4Ppc44hcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BifCBzMECgeW0h[W[2ZYKgOFghcHK\|	M{O4UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N\|A6ODxxYU6=
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NIH3T3	MVzGeY5kfGmxbjDhd5NigQ>?	Mmj5NVAxKHWP	MnzEOFghcHK\|		MUHS[YR2[3Srb36gc4Yh[3mlbHnuJGQyKGenbnWg[ZhxemW\|c3nvckBqdiC4LWPyZ{B1emGwc3\lZ5Rm\CCvb4Xz[UBPUUh\|VEOgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDR6IHjydy=>	NWT5OVNtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
NIH3T3	NVfnem1WTnWwY4Tpc44h[XO\|YYm=	NVXRNYV1OTByIIXN	NFf0RmM1QCCqcoO=		Ml\0VoVlfWO2aX;uJI9nKEKlbD34UEBo\W6nIHX4dJJme3Orb36gbY4hfi2VcnOgeJJidnOoZXP0[YQhdW:3c3WgUmlJO1R\|IHPlcIx{KGG2IEGwNEB2VSCjZoTldkA1QCCqcoO=	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
NIH3T3	NIS5WpVHfW6ldHnvckBie3OjeR?=	M{D6WlExOCC3TR?=	NHPPRoY1QCCqcoO=		Mkn2VoVlfWO2aX;uJI9nKHO3co\peolvKGenbnWg[ZhxemW\|c3nvckBqdiC4LWPyZ{B1emGwc3\lZ5Rm\CCvb4Xz[UBPUUh\|VEOgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDR6IHjydy=>	MmfFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-231	MVPGeY5kfGmxbjDhd5NigQ>?	NIjobJUyODBidV2=	NFfabFQ1QCCqcoO=		MlfCVoVlfWO2aX;uJI9nKEKlbD34UEBo\W6nIHX4dJJme3Orb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDheEAyODBidV2gZYZ1\XJiNEigbJJ{	NHPFbZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N\|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-231	NFfCS3hHfW6ldHnvckBie3OjeR?=	M4nT[lExOCC3TR?=	NF7EVXc1QCCqcoO=		Mlz4VoVlfWO2aX;uJI9nKHO3co\peolvKGenbnWg[ZhxemW\|c3nvckBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF1KDFyMDD1UUBi\nSncjC0PEBpenN?	M3zVZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N\|A6ODxxYU6=
MDA-MB-231	MlXoRY51cXS3bX;yJIF{e2G7	M4D6WlUhdWdxa3e=	MmrRNkB4\WWtcx?=		NWPwc4lKSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDlfJBz\XO\|aX7nJIFkfGm4ZTDTeIF1OyC6ZX7v[5Ji\nSnZDDpckBud3W\|ZTDheEA2KG2pL3vnMEBqfiCob4Kg[ZZmenliMzDkZZl{KG[xcjCyJJdm\Wu\|	NVrpdZR1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
NIH3T3	NFXMUYdIem:5dHigbY5pcWKrdHnvckBie3OjeR?=	MoWzNVAxKHWP			MlP4S5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJO1R\|IHPlcIx{KGW6cILld5Nqdmdidj3TdoMh[XRiMUCwJJVOKG[xcjDleoVzgSB\|IHThfZMh[nlic3;meE1i\2G{IHPvcI9vgS2ob4LtZZRqd25iYYPzZZk>	NHPqe409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N\|A6OCd-MUe0OlMxQTB:L3G+
A673	NUHIRm92eUiWUzDhd5NigQ>?				MVjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>?	NWLmb4x[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC	NHHjenlyUFSVIHHzd4F6				M4XiUZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz	NYLwS3R4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643	NEDaUlJyUFSVIHHzd4F6				Mkj5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?=	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
LAN-5	M3PhWJFJXFNiYYPzZZk>				NXzGfJlTeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IFzBUk02KGOnbHzz	MkHyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=

Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID

Western blot	PD-L1 STAT3 / p-STAT3 / Cyclin D1 / Bcl-2 / Nanog / OCT4 / ALDH1 / CD44	28605599 26556875
Immunofluorescence	Oct4 / Twist p-STAT3	27521216 26556875
Growth inhibition assay	Cell viability	26813676

体内研究

5 mg/kg剂量的S3I-201作用于携带人类乳腺癌(MDA-MB-231)的鼠时，显示出强抑制效果。^[1]5 mg/kg剂量的S3I-201作用于Huh-7移植瘤，显示出强抗癌活性，没有明显的外部健康改变或者体重减轻症状。^[2]

激酶实验：^[1]	体外Stat3 DNA结合实验和EMSA分析: 100 mL生物素-e-Ac-EPQpYEEIEL-OH (溶于50 mM Tris/150 mM NaCl, pH 为7.5) 加到链霉亲和素包被的96孔微型板的每孔中，在4^oC下振荡温育过夜。用PBS/Tween-20冲洗板，然后加入2次200 mL BSA-T-PBS (0.2% BSA/0.1% Tween-20/PBS)。50 mL Lck-SH2-GST 融合蛋白(6.4 ng/ml，溶于BSA-T-PBS中)加到96孔板的每孔中，在室温下振荡处理4小时。转移溶液，用200 mL BSA-T-PBS冲洗4次,然后在每孔中加入100 mL多克隆GST抗体 (100 ng/mL，溶于BSA-T-PBS)，4^oC下温育过夜。用BSA-T-PBS冲洗后, 在每孔中加入100 mL 200 ng/ml BSA-T-PBS辣根过氧化物酶结合的二抗，然后在室温下温育45分钟。用BSA-T-PBS冲洗4步后，再用PBS-T冲洗3步，然后在每孔中加入100 mL过氧化物酶底物，温育5到15分钟。加入100 mL 1 M硫酸溶液终止过氧化物酶反应，使用ELISA计数板在450nm 处读取吸光值。
细胞实验：^[2]	Cell lines: HCC细胞系包括HepG2, PLC/PRF/5, SNU-449, Huh-7, SNU-398, SNU-182，和SNU-475 Concentrations: 25到250 μM Incubation Time: 72小时 Method: 5 ×10³个细胞接种在含完全培养基的96孔微型板上。培养72小时后，每孔加入100 μL 2 mg/mL MTT溶液测量存活细胞数。2小时后，转移培养基，每孔加入100 μL二甲亚砜溶解甲结晶。使用酶联免疫吸附法计数器在590 nm处读取吸光值。
动物实验：^[1]	Animal Models: 6周大的携带MDA-MB-231移植瘤模型的雌性无胸腺裸鼠 Dosages: 5 mg/kg Administration: 静脉注射，每周2天或3天。

参考文献	[1] Siddiquee K, et al. Proc Natl Acad Sci U S A, 2007, 104(18), 7391-7396. [2] Lin L, et al. Oncogene, 2009, 28(7), 961-972. [3] Chen W, et al. Liver Int, 2012, 32(1), 70-77. [4] Sen N, et al. Proc Natl Acad Sci U S A, 2012, 109(2), 600-605. + 展开

溶解度（25°C）

体外


体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 5% DMSO+corn oil 点击购买： Corn Oil	3mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量	365.36
化学式	C₁₆H₁₅NO₇S
CAS号	501919-59-1
储存条件	3年	-20°C	粉状
储存条件	2年	-80°C	溶于溶剂

Download S3I-201 SDF

动物体内配方计算器（澄清溶液）

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 μL 动物数量只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO + % + % Tween 80 + % ddH2O

%DMSO+ %

计算结果：

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于μL DMSO溶液（母液浓度mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取μL DMSO母液，加入μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入μL ddH₂O，混匀澄清。

体内配方配制方法：取μL DMSO母液，加入μL Corn oil，混匀澄清。

注意：1. 首先保证母液是澄清的；
2.一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×

稀释计算器分子量计算器

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