S3I-201

目录号:S1155 别名: NSC 74859

仅限科研使用

S3I-201 (NSC 74859) 有效抑制STAT3的DNA结合活性,IC50为86 μM,而对STAT1和STAT5的抑制活性低。

S3I-201 Chemical Structure

CAS: 501919-59-1

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 638.93 现货
RMB 563.02 现货
RMB 972.36 现货
RMB 3028.28 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的S3I-201发表文献197篇:

产品安全说明书

STAT抑制剂选择性比较

相关STAT产品

生物活性

产品描述 S3I-201 (NSC 74859) 有效抑制STAT3的DNA结合活性,IC50为86 μM,而对STAT1和STAT5的抑制活性低。
特性 S3I-201是化学探测器抑制剂,可以除去乳腺瘤。
靶点
STAT3 [1]
(Cell-free assay)
86 μM
体外研究

S3I-201选择性抑制Stat3 DNA结合活性。S3I-201抑制Stat3·Stat3复合形式,不依赖于Stat3的激活状态。S3I-201不干涉Lck SH2与同源pTyr肽结合。S3I-201作用于NIH 3T3/v-Src鼠成纤维细胞和人类乳腺癌MDA-MB-231, MDA-MB-435, 和MDA-MB-468 细胞,可以抑制Stat3激活。S3I-201 抑制Stat3依赖的转录活性。 S3I-201 也抑制编码cyclin D1, Bcl-xL,和surviving 的Stat3-调节基因的表达。[1]S3I-201可以降低 pS727STAT3水平和降低TGF-β通路蛋白水平。S3I-201也可抑制CD133+ 和CD133− Huh-7细胞。[2]最新研究显示S3I-201作用于Hep-G2, Huh-7和SK-HEP-1细胞,加强 Cetuximab的抗增殖效果。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U87 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2Lld|czKGh? M3rTUWlEPTB;NUWuNUDPxE1? Ml3aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByN{K2OVIoRjJyMEeyOlUzRC:jPh?=
U373  MnHZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\XO|IhcA>? MnnuTWM2OD13Mj61JO69VQ>? NGnaUoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC3NlY2Oid-MkCwO|I3PTJ:L3G+
HPAC NHHqTVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MknoO|IhcA>? NX7KVWRMUUN3MP-8olExOCEQvF2= M3;O[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEeyOlUzLz5{MEC3NlY2OjxxYU6=
PANC-1 M{HzXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NInxTZg4OiCq MX3JR|Ux97zgMUCwJO69VQ>? M4fRfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEeyOlUzLz5{MEC3NlY2OjxxYU6=
SK-BR-3 NUXpfm1OT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnLEO|IhcA>? M{n3Z2lEPTExvK6xNFAh|ryP NWfqOo1sRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwO|I3PTJpPkKwNFczPjV{PD;hQi=>
 U-373 MG NUL1bml3S3m2b4TvfIlkcXS7IFHzd4F6 MnO5N{8yOCEQvF2= MX2yOEBp MWfy[YR2[2W|IF\OMe6{NWmwZIXj[YQh[2WubDDu[ZVzd3SxeHnjbZR6 NWXsRYx6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4PFg1OTZpPkKwPFg5PDF4PD;hQi=>
MDA-MB-231 NF72[HJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nLV|czKGh? M2rSR2lEPTExvK6xNFAh|ryP NH;NN3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC3NlY2Oid-MkCwO|I3PTJ:L3G+
HUVEC  NH2z[INHfW6ldHnvckBCe3OjeR?= NHjEPXkxNjVvMkCg{txO MYOyOEBp Mm\ESG1UVw>? NHHBSld{fXCycnXzd4V{KHSqZTDofZBwgGmjLXnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEiLRj2x{tE> M{LFS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUKzOVU6Lz5{MUWyN|U2QTxxYU6=
Huh7 M1nCZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXTNVAxKG6P MVW0PEBp NWjJeFZ6TE2VTx?= Ml6wbY5pcWKrdIOgeIhmKEmOLU[gd5RqdXWuYYTpc44heHKxbX;0[YQh[2WubDDwdo9tcW[ncnH0bY9v MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN4NEO4PUc,OjN|NkSzPFk9N2F-
PLC/PRF/5  NGfp[FRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1\LS|ExOCCwTR?= MkLYOFghcA>? M{TxXWROW09? MXnpcohq[mm2czD0bIUhUUxvNjDzeIlufWyjdHnvckBxem:vb4Tl[EBk\WyuIIDyc4xq\mW{YYTpc44> MnvuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|NkSzPFkoRjJ|M{[0N|g6RC:jPh?=
H460  M3f4V2Z2dmO2aX;uJGF{e2G7 MXG1NE8yODBizszN NEHpZXY1QCCq NGrq[5BqdmirYnn0d{B1cGViU4TheFNEKGmwY4LlZZNm\CCvaWKtPVJiKGW6cILld5Nqd25? NH\o[YU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{iyNFI2PCd-MkO4NlAzPTR:L3G+
H1299 M2TsUWZ2dmO2aX;uJGF{e2G7 NIniVG82OC9zMECg{txO M12zWlQ5KGh? Mo\hd5VxeHKnc4Pld{BucVJvOULhJIV5eHKnc4Ppc44h\G:|ZT3k[ZBmdmSnboTsfS=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh{MEK1OEc,OjN6MkCyOVQ9N2F-
T-cell  NU\iPWM6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn;CTWM2OD13MDFOwG0> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB4OEezNUc,OjRyNki3N|E9N2F-
U373  Mln6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYqxNlUh|ryP NWf2S2xMOjRiaB?= MmX0SG1UVw>? MmXX[Il{enWydIOgV3RCXDNic3nncoFtcW6pIHHu[EBxem:uaX\ldoF1cW:w M3z0elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEewPFIxLz5{NEC3NFgzODxxYU6=
HUT-102 NWDSV2ZwSXCxcITvd4l{KEG|c3H5 Mn;DO|UuOzByIN88US=> M{TRelI1NzR6IHi= NXn6Zpdne3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25iaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJiYX7kJIlv\HWlZYOgZ4VtdCCjcH;weI9{cXQEoB?= MmHlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUC5PVUoRjJ2MEmwPVk2RC:jPh?=
MT-2 NV6xcGNqSXCxcITvd4l{KEG|c3H5 NH3MOW44PS1|MECg{txO MVKyOE81QCCq NHjlWWZ{fXCycnXzd4V{KGOnbHygdJJwdGmoZYLheIlwdiCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meiCjbnSgbY5lfWOnczDj[YxtKGGyb4D0c5Nqe8Li NFy5eYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5NFk6PSd-MkSwPVA6QTV:L3G+
H460  NX6zR29CSXCxcITvd4l{KEG|c3H5 M2HKcVExOMLibl2= NWDV[IFXOjUEoHi= NEnqXYhmdmijbnPld{Bk\WyuIHTlZZRpKGOxLYTy[YF1\WRid3n0bEBNYTJ7NECwNi=> NVOzOFA2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|I2OzhpPkK0OFczPTN6PD;hQi=>
A459 MXfBdI9xfG:|aYOgRZN{[Xl? MUexNFDDqG6P M4jZZlI1yqCq M1\HNYlv\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3:teJJm[XSnZDD3bZRpKEKHWkKzOS=> MlHsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2N{K1N|goRjJ2NEeyOVM5RC:jPh?=
H460  NVL6Zm9{SXCxcITvd4l{KEG|c3H5 M1PyVlExOMLibl2= NH7EZlYzPMLiaB?= M3Pyeolv\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3:teJJm[XSnZDD3bZRpKEKHWkKzOS=> M3\nRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEeyOVM5Lz5{NES3NlU{QDxxYU6=
GC  M1;Tdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M121ZlUxNTF{NTFOwG0> M4LxVFczKGh? NVr0bFRF[XS2ZX71ZZRmeyC2aHWgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M1vJTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{e0OVA{Lz5{NUe3OFUxOzxxYU6=
GH3 NYPSbmZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofDOVAuOTJ3IN88US=> NFLL[VU4OiCq MXjheJRmdnWjdHXzJJRp\SClZXzsJIdzd3e2aDDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NEnrWHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe3OFUxOyd-MkW3O|Q2ODN:L3G+
BT474R  M3niSWZ2dmO2aX;uJGF{e2G7 M3e4XlUxKM7:TR?= M1LsTFExNTZyIHS= NXPFVYh6cW6qaXLpeJMhW1SDVEOgZYN1cX[rdIm= NH7oO209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOyO|U3OSd-MkWzNlc2PjF:L3G+
NCI-N87R NGf1WFBHfW6ldHnvckBCe3OjeR?= MnHBOVAh|ryP MmfGNVAuPjBiZB?= MmGzbY5pcWKrdIOgV3RCXDNiYXP0bZZqfHl? M4[wWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{K3OVYyLz5{NUOyO|U3OTxxYU6=
MDA-MB-468 M1y1NGZ2dmO2aX;uJIF{e2G7 NFjoWWYyODBidV2= M2r1R|I1KGi{cx?= MY\Jcohq[mm2aX;uJI9nKFO2YYSzJIFkfGm4YYTpc44hcW5iaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK| MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-435 MmC0SpVv[3Srb36gZZN{[Xl? MWCxNFAhfU1? NF;GZnIzPCCqcoO= MUTJcohq[mm2aX;uJI9nKFO2YYSzJIFkfGm4YYTpc44hcW5iaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK| MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-231 M1HBfWZ2dmO2aX;uJIF{e2G7 NWjYb45{OTByIIXN NVm1[Zh4OjRiaILz MWnJcohq[mm2aX;uJI9nKFO2YYSzJIFkfGm4YYTpc44hcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK| MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
NIH3T3 NYPQWJV5TnWwY4Tpc44h[XO|YYm= NG\vblMyODBidV2= MoD1NlQhcHK| M4DT[3Jm\HWldHnvckBw\iCyVInyMVcxPSCVdHH0N{Bt\X[nbDDpckB3NVO{YzDlfJBz\XO|aX7nJI1wfXOnIF7JTFNVOyClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK| MlnRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
NIH3T3 MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NF6zWFYyODBidV2= MUW0JIRigXN? MUnHdo94fGhiaX7obYJqfGmxbjDv[kBud3W|ZTDOTWg{XDNiY3XscJMh\XiycnXzd4lv\yC4LWPyZ{BifCBzMECgeW0h[W[2ZYKgOEBl[Xm|IHL5JJRzgXCjbjDicJVmKGW6Y3z1d4lwdiCjc4PhfS=> M1W1U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
MDA-MB-435 NFrIXHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NU\DN|VxOTByIIXN NUn4PZZDPCCmYYnz MYrHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDNSGEuVUJvNEO1JINmdGy|IHX4dJJme3Orbnegek1UemNiYYSgNVAxKHWPIHHmeIVzKDRiZHH5d{BjgSC2conwZY4h[my3ZTDlfINtfXOrb36gZZN{[Xl? NYKxSWdSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
MDA-MB-231 M1m1PWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEX3W|YyODBidV2= MWe0JIRigXN? NVX4R5RiT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BmgHC{ZYPzbY5oKHZvU4LjJIF1KDFyMDD1UUBi\nSncjC0JIRigXNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG|c3H5 NF2xdYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-468 Mlu1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYSxNFAhfU1? Ml\rOEBl[Xm| NX;3NpVXT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{BmgHC{ZYPzbY5oKHZvU4LjJIF1KDFyMDD1UUBi\nSncjC0JIRigXNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG|c3H5 MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
NIH3T3 M1fMWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVGxNFAhfU1? NF3ab|BIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBPUUh|VEOgZ4VtdHNiZYjwdoV{e2mwZzD2MXJieyCjdDCxNFAhfU1iZn;yJIV3\XK7IEOg[IF6eyCkeTDzc4Z1NWGpYYKgZ49td267LX\vdo1ifGmxbjDhd5NigQ>? NELjOpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-435 NEXnbmJCeG:ydH;zbZMh[XO|YYm= MkPUN|AhfG9iMUCwJJVO MUS0PEBpenN? MonDTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCPRFGtUWIuPDN3IHPlcIx{KGW6cILld5NqdmdiYXP0bZZmKFO2YYSzJIF1KDNyIITvJFExOCC3TTDh[pRmeiB2ODDodpM> NHu2cXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-231 NVmyUIN1SXCxcITvd4l{KGG|c3H5 M1HjVlExOCC3TR?= NH;JWpkzPCCqcoO= MWXS[YR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIGP0ZZQ{KHS{YX7z[oVkfGWmIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiMUCwJJVOKGGodHXyJFI1KGi{cx?= NFi5ZXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
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MDA-MB-231 NWXUVpBWSXCxcITvd4l{KGG|c3H5 NFnESZgyODBidV2= NGniN3ozPCCqcoO= NFzrSnpKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJHN1[XR|IGPINkBld22jaX6geJJidnOoZXP0[YQhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDheEAyODBidV2gZYZ1\XJiMkSgbJJ{ MlToQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
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NIH3T3 MVzGeY5kfGmxbjDhd5NigQ>? Mmj5NVAxKHWP MnzEOFghcHK| MUHS[YR2[3Srb36gc4Yh[3mlbHnuJGQyKGenbnWg[ZhxemW|c3nvckBqdiC4LWPyZ{B1emGwc3\lZ5Rm\CCvb4Xz[UBPUUh|VEOgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDR6IHjydy=> NWT5OVNtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
NIH3T3 NVfnem1WTnWwY4Tpc44h[XO|YYm= NVXRNYV1OTByIIXN NFf0RmM1QCCqcoO= Ml\0VoVlfWO2aX;uJI9nKEKlbD34UEBo\W6nIHX4dJJme3Orb36gbY4hfi2VcnOgeJJidnOoZXP0[YQhdW:3c3WgUmlJO1R|IHPlcIx{KGG2IEGwNEB2VSCjZoTldkA1QCCqcoO= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
NIH3T3 NIS5WpVHfW6ldHnvckBie3OjeR?= M{D6WlExOCC3TR?= NHPPRoY1QCCqcoO= Mkn2VoVlfWO2aX;uJI9nKHO3co\peolvKGenbnWg[ZhxemW|c3nvckBqdiC4LWPyZ{B1emGwc3\lZ5Rm\CCvb4Xz[UBPUUh|VEOgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDR6IHjydy=> MmfFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-231 MVPGeY5kfGmxbjDhd5NigQ>? NIjobJUyODBidV2= NFfabFQ1QCCqcoO= MlfCVoVlfWO2aX;uJI9nKEKlbD34UEBo\W6nIHX4dJJme3Orb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDheEAyODBidV2gZYZ1\XJiNEigbJJ{ NHPFbZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-231 NFfCS3hHfW6ldHnvckBie3OjeR?= M4nT[lExOCC3TR?= NF7EVXc1QCCqcoO= Mlz4VoVlfWO2aX;uJI9nKHO3co\peolvKGenbnWg[ZhxemW|c3nvckBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIF1KDFyMDD1UUBi\nSncjC0PEBpenN? M3zVZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
MDA-MB-231 MlXoRY51cXS3bX;yJIF{e2G7 M4D6WlUhdWdxa3e= MmrRNkB4\WWtcx?= NWPwc4lKSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDlfJBz\XO|aX7nJIFkfGm4ZTDTeIF1OyC6ZX7v[5Ji\nSnZDDpckBud3W|ZTDheEA2KG2pL3vnMEBqfiCob4Kg[ZZmenliMzDkZZl{KG[xcjCyJJdm\Wu| NVrpdZR1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
NIH3T3 NFXMUYdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoWzNVAxKHWP MlP4S5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJO1R|IHPlcIx{KGW6cILld5Nqdmdidj3TdoMh[XRiMUCwJJVOKG[xcjDleoVzgSB|IHThfZMh[nlic3;meE1i\2G{IHPvcI9vgS2ob4LtZZRqd25iYYPzZZk> NHPqe409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
A673 NUHIRm92eUiWUzDhd5NigQ>? MVjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? NWLmb4x[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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NB1643 NEDaUlJyUFSVIHHzd4F6 Mkj5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6EMU[0N{Bk\Wyucx?= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
LAN-5 M3PhWJFJXFNiYYPzZZk> NXzGfJlTeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFzBUk02KGOnbHzz MkHyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Assay
Methods Test Index PMID
Western blot PD-L1 STAT3 / p-STAT3 / Cyclin D1 / Bcl-2 / Nanog / OCT4 / ALDH1 / CD44 28605599 26556875
Immunofluorescence Oct4 / Twist p-STAT3 27521216 26556875
Growth inhibition assay Cell viability 26813676
体内研究 5 mg/kg剂量的S3I-201作用于携带人类乳腺癌(MDA-MB-231)的鼠时,显示出强抑制效果。[1]5 mg/kg剂量的S3I-201作用于Huh-7移植瘤,显示出强抗癌活性,没有明显的外部健康改变或者体重减轻症状。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
  • 体外Stat3 DNA结合实验和EMSA分析:

    100 mL生物素-e-Ac-EPQpYEEIEL-OH (溶于50 mM Tris/150 mM NaCl, pH 为7.5) 加到链霉亲和素包被的96孔微型板的每孔中,在4oC下振荡温育过夜。用PBS/Tween-20冲洗板,然后加入2次200 mL BSA-T-PBS (0.2% BSA/0.1% Tween-20/PBS)。50 mL Lck-SH2-GST 融合蛋白(6.4 ng/ml,溶于BSA-T-PBS中)加到96孔板的每孔中,在室温下振荡处理4小时。转移溶液,用200 mL BSA-T-PBS冲洗4次,然后在每孔中加入100 mL多克隆GST抗体 (100 ng/mL,溶于BSA-T-PBS),4oC下温育过夜。用BSA-T-PBS冲洗后, 在每孔中加入100 mL 200 ng/ml BSA-T-PBS辣根过氧化物酶结合的二抗,然后在室温下温育45分钟。用BSA-T-PBS冲洗4步后,再用PBS-T冲洗3步,然后在每孔中加入100 mL过氧化物酶底物,温育5到15分钟。加入100 mL 1 M硫酸溶液终止过氧化物酶反应,使用ELISA计数板在450nm 处读取吸光值。

细胞实验:[2]
  • Cell lines: HCC细胞系包括HepG2, PLC/PRF/5, SNU-449, Huh-7, SNU-398, SNU-182,和SNU-475
  • Concentrations: 25到250 μM
  • Incubation Time: 72小时
  • Method: 5 ×103个细胞接种在含完全培养基的96孔微型板上。培养72小时后,每孔加入100 μL 2 mg/mL MTT溶液测量存活细胞数。2小时后,转移培养基,每孔加入100 μL二甲亚砜溶解甲结晶。使用酶联免疫吸附法计数器在590 nm处读取吸光值。
动物实验:[1]
  • Animal Models: 6周大的携带MDA-MB-231移植瘤模型的雌性无胸腺裸鼠
  • Dosages: 5 mg/kg
  • Administration: 静脉注射,每周2天或3天。

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+corn oil

3mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 365.36
化学式

C16H15NO7S

CAS号 501919-59-1
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

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操作手册

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