S3I-201

For research use only. Not for use in humans.

目录号:S1155 别名: NSC 74859

S3I-201 Chemical Structure

CAS No. 501919-59-1

S3I-201 (NSC 74859) 有效抑制STAT3的DNA结合活性,IC50为86 μM,而对STAT1和STAT5的抑制活性低。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 638.93 现货
RMB 563.02 现货
RMB 972.36 现货
RMB 3028.28 现货
RMB 8165.43 现货
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客户使用Selleck生产的S3I-201发表文献191篇:

产品安全说明书

STAT抑制剂选择性比较

生物活性

产品描述 S3I-201 (NSC 74859) 有效抑制STAT3的DNA结合活性,IC50为86 μM,而对STAT1和STAT5的抑制活性低。
特性 S3I-201是化学探测器抑制剂,可以除去乳腺瘤。
靶点
STAT3 [1]
(Cell-free assay)
86 μM
体外研究

S3I-201选择性抑制Stat3 DNA结合活性。S3I-201抑制Stat3·Stat3复合形式,不依赖于Stat3的激活状态。S3I-201不干涉Lck SH2与同源pTyr肽结合。S3I-201作用于NIH 3T3/v-Src鼠成纤维细胞和人类乳腺癌MDA-MB-231, MDA-MB-435, 和MDA-MB-468 细胞,可以抑制Stat3激活。S3I-201 抑制Stat3依赖的转录活性。 S3I-201 也抑制编码cyclin D1, Bcl-xL,和surviving 的Stat3-调节基因的表达。[1]S3I-201可以降低 pS727STAT3水平和降低TGF-β通路蛋白水平。S3I-201也可抑制CD133+ 和CD133− Huh-7细胞。[2]最新研究显示S3I-201作用于Hep-G2, Huh-7和SK-HEP-1细胞,加强 Cetuximab的抗增殖效果。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U87 NHLmTZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYq1OXZyPzJiaB?= NVzNNVBjUUN3ME21OU4yKM7:TR?= NXfRdItrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwO|I3PTJpPkKwNFczPjV{PD;hQi=>
U373  NY\Nb3ZoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\nW3o4OiCq MUTJR|UxRTV{LkWg{txO M{G4[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEeyOlUzLz5{MEC3NlY2OjxxYU6=
HPAC NWrtWlZHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDIUId6PzJiaB?= MojoTWM2OO,:nkGwNEDPxE1? NVzkUopkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwO|I3PTJpPkKwNFczPjV{PD;hQi=>
PANC-1 Mn3FS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTPPVU4OiCq NGPuVIdKSzVy78{eNVAxKM7:TR?= NYTmdY4{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwO|I3PTJpPkKwNFczPjV{PD;hQi=>
SK-BR-3 M3i2Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDPdYVPPzJiaB?= NF;hUZJKSzVy78{eNVAxKM7:TR?= NWXWTGlqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwO|I3PTJpPkKwNFczPjV{PD;hQi=>
 U-373 MG NH36RmZEgXSxdH;4bYNqfHliQYPzZZk> MnH3N{8yOCEQvF2= NU\2c295OjRiaB?= M2fwS5Jm\HWlZYOgSm4u|rNvaX7keYNm\CClZXzsJI5mfXKxdH;4bYNqfHl? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh6OESxOkc,OjB6OEi0NVY9N2F-
MDA-MB-231 MmDFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrHemQ4OiCq MV\JR|Ux97zgMUCwJO69VQ>? M2j3TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEeyOlUzLz5{MEC3NlY2OjxxYU6=
HUVEC  M4LacWZ2dmO2aX;uJGF{e2G7 NVjZSWs1OC53LUKwJO69VQ>? MlXYNlQhcA>? NFPuenFFVVOR M3T5VpN2eHC{ZYPz[ZMhfGinIHj5dI95cWFvaX7keYNm\CCjY3P1cZVt[XSrb36gc4YhUEmILUJOtS=> Mn3iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MkO1OVkoRjJzNUKzOVU6RC:jPh?=
Huh7 MlK2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnhZpoyODBibl2= NWe1TXNiPDhiaB?= Ml;ISG1UVw>? Mm\obY5pcWKrdIOgeIhmKEmOLU[gd5RqdXWuYYTpc44heHKxbX;0[YQh[2WubDDwdo9tcW[ncnH0bY9v NHThO|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O2OFM5QSd-MkOzOlQ{QDl:L3G+
PLC/PRF/5  NGfP[GpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\RNVAxKG6P NXq5[ok4PDhiaB?= NEjMcnRFVVOR M2riSYlvcGmkaYTzJJRp\SCLTD22JJN1cW23bHH0bY9vKHC{b33veIVlKGOnbHygdJJwdGmoZYLheIlwdg>? M37yclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{[0N|g6Lz5{M{O2OFM5QTxxYU6=
H460  M1n3SGZ2dmO2aX;uJGF{e2G7 MXy1NE8yODBizszN MnzpOFghcA>? NHftbVJqdmirYnn0d{B1cGViU4TheFNEKGmwY4LlZZNm\CCvaWKtPVJiKGW6cILld5Nqd25? M4TF[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEKwNlU1Lz5{M{iyNFI2PDxxYU6=
H1299 NInhOWRHfW6ldHnvckBCe3OjeR?= M1OzclUxNzFyMDFOwG0> NGHrbHA1QCCq M124[JN2eHC{ZYPz[ZMhdWmULUmyZUBmgHC{ZYPzbY9vKGSxc3Wt[IVx\W6mZX70cJk> MnnLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MkCyOVQoRjJ|OEKwNlU1RC:jPh?=
T-cell  NVz4SXVFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4Luc2lEPTB;NUCg{txO M2fKNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2ME[4O|MyLz5{NEC2PFc{OTxxYU6=
U373  MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjQWW1ZOTJ3IN88US=> MmjHNlQhcA>? NVjyXYF3TE2VTx?= MWjkbZNzfXC2czDTWGFVOyC|aXfuZYxqdmdiYX7kJJBzd2yrZnXyZZRqd25? MnTVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyN{C4NlAoRjJ2MEewPFIxRC:jPh?=
HUT-102 MW\BdI9xfG:|aYOgRZN{[Xl? MXy3OU0{ODBizszN MUeyOE81QCCq M1;SeZN2eHC{ZYPz[ZMh[2WubDDwdo9tcW[ncnH0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVzKGGwZDDpcoR2[2W|IHPlcIwh[XCxcITvd4l{yqB? NYO0RZRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVA6QTVpPkK0NFkxQTl3PD;hQi=>
MT-2 M13lRmFxd3C2b4Ppd{BCe3OjeR?= NVnrV|ZRPzVvM{CwJO69VQ>? M3fRZlI1NzR6IHi= M{\NeZN2eHC{ZYPz[ZMh[2WubDDwdo9tcW[ncnH0bY9vKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVzKGGwZDDpcoR2[2W|IHPlcIwh[XCxcITvd4l{yqB? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7MEm5OUc,OjRyOUC5PVU9N2F-
H460  M{TMSGFxd3C2b4Ppd{BCe3OjeR?= MmfjNVAxyqCwTR?= M{\wVFI1yqCq NHzsOHpmdmijbnPld{Bk\WyuIHTlZZRpKGOxLYTy[YF1\WRid3n0bEBNYTJ7NECwNi=> NGLW[FI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES3NlU{QCd-MkS0O|I2Ozh:L3G+
A459 MUTBdI9xfG:|aYOgRZN{[Xl? NGrOUWgyODEEoH7N MoDYNlTDqGh? M{i2Nolv\HWlZYOgZ4VtdCCjcH;weI9{cXNiY3:teJJm[XSnZDD3bZRpKEKHWkKzOS=> NV;hNW01RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|I2OzhpPkK0OFczPTN6PD;hQi=>
H460  NF7zWFZCeG:ydH;zbZMhSXO|YYm= NG\HOWkyODEEoH7N NX65TYpzOjUEoHi= Ml\YbY5lfWOnczDj[YxtKGGyb4D0c5NqeyClbz30doVifGWmIIfpeIghSkWcMkO1 MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR5MkWzPEc,OjR2N{K1N|g9N2F-
GC  M3qyNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrnWVU2OC1zMkWg{txO MmTqO|IhcA>? M2jaV4F1fGWwdXH0[ZMhfGinIHPlcIwh\3Kxd4ToJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M1HxS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{e0OVA{Lz5{NUe3OFUxOzxxYU6=
GH3 NF\ldZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rKeVUxNTF{NTFOwG0> Mn3HO|IhcA>? MlnFZZR1\W63YYTld{B1cGViY3XscEBoem:5dHigbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NYP5WG9CRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3O|Q2ODNpPkK1O|c1PTB|PD;hQi=>
BT474R  MonoSpVv[3Srb36gRZN{[Xl? M{nx[FUxKM7:TR?= NYnDb4RqOTBvNkCg[C=> M{GxfolvcGmkaYTzJHNVSVR|IHHjeIl3cXS7 NXHXSZQ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNlc2PjFpPkK1N|I4PTZzPD;hQi=>
NCI-N87R M3PqbGZ2dmO2aX;uJGF{e2G7 MknzOVAh|ryP NVzGXpFZOTBvNkCg[C=> NWP5UGJucW6qaXLpeJMhW1SDVEOgZYN1cX[rdIm= M2nPclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{K3OVYyLz5{NUOyO|U3OTxxYU6=
MDA-MB-468 NWP3e49WTnWwY4Tpc44h[XO|YYm= MlriNVAxKHWP MV2yOEBpenN? MUTJcohq[mm2aX;uJI9nKFO2YYSzJIFkfGm4YYTpc44hcW5iaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK| Mk\tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-435 NG\ob3VHfW6ldHnvckBie3OjeR?= M1jnUVExOCC3TR?= NXzDU5RyOjRiaILz MX7Jcohq[mm2aX;uJI9nKFO2YYSzJIFkfGm4YYTpc44hcW5iaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{BifCBzMECgeW0h[W[2ZYKgNlQhcHK| MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-231 NU[4WlJWTnWwY4Tpc44h[XO|YYm= M{jWTFExOCC3TR?= MXKyOEBpenN? NGmz[IJKdmirYnn0bY9vKG:oIGP0ZZQ{KGGldHn2ZZRqd25iaX6gbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjdDCxNFAhfU1iYX\0[ZIhOjRiaILz MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
NIH3T3 NWKwXXZxTnWwY4Tpc44h[XO|YYm= M1PkelExOCC3TR?= NWe5N|JwOjRiaILz NGXQWHZT\WS3Y4Tpc44hd2ZicGT5dk04ODViU4TheFMhdGW4ZXygbY4hfi2VcnOg[ZhxemW|c3nu[{Bud3W|ZTDOTWg{XDNiY3XscJMh[XRiMUCwJJVOKGGodHXyJFI1KGi{cx?= M1XFVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
NIH3T3 MWTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1\Ud|ExOCC3TR?= MYi0JIRigXN? NH3sWWNIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBPUUh|VEOgZ4VtdHNiZYjwdoV{e2mwZzD2MXNz[yCjdDCxNFAhfU1iYX\0[ZIhPCCmYYnzJIJ6KHS{eYDhckBjdHWnIHX4Z4x2e2mxbjDhd5NigQ>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-435 M3zZdmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M3L3dFExOCC3TR?= MkHoOEBl[Xm| NF7lbIdIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBOTEFvTVKtOFM2KGOnbHzzJIV5eHKnc4Ppcochfi2VcnOgZZQhOTByIIXNJIFnfGW{IESg[IF6eyCkeTD0dplx[W5iYnz1[UBmgGOudYPpc44h[XO|YYm= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-231 NFG5ZZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYqxNFAhfU1? NVHLWFlmPCCmYYnz NYLvem1ET3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BmgHC{ZYPzbY5oKHZvU4LjJIF1KDFyMDD1UUBi\nSncjC0JIRigXNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG|c3H5 M4DP[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
MDA-MB-468 MmrlS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGjZSlQyODBidV2= MYi0JIRigXN? M2nDTWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1FSS2PQj20Olgh[2WubIOg[ZhxemW|c3nu[{B3NVO{YzDheEAyODBidV2gZYZ1\XJiNDDkZZl{KGK7IITyfZBidiCkbIXlJIV5[2y3c3nvckBie3OjeR?= M{HLOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
NIH3T3 NWXS[XBxT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVexNFAhfU1? M37mbWdzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJG5KUDOWMzDj[YxteyCneIDy[ZN{cW6pII[tVoF{KGG2IEGwNEB2VSCob4Kg[ZZmenliMzDkZZl{KGK7IIPv[pQu[WejcjDjc4xwdnlvZn;ycYF1cW:wIHHzd4F6 Mkn4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-435 MUPBdI9xfG:|aYOgZZN{[Xl? MnWyN|AhfG9iMUCwJJVO MX[0PEBpenN? NVjxOm1SUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDNSGEuVUJvNEO1JINmdGy|IHX4dJJme3OrbnegZYN1cX[nIGP0ZZQ{KGG2IEOwJJRwKDFyMDD1UUBi\nSncjC0PEBpenN? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-231 MkTVRZBweHSxc3nzJIF{e2G7 NYH4dFVQOTByIIXN M4L3dlI1KGi{cx?= NXzBflJRWmWmdXP0bY9vKG:oIHHwc5B1d3OrczDpckBUfGG2MzD0doFve2[nY4Tl[EBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHH0JFExOCC3TTDh[pRmeiB{NDDodpM> MnzFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-231 MVnGeY5kfGmxbjDhd5NigQ>? MkfDNVAxKHWP MluwOFghcHK| NGfGS3dT\WS3Y4Tpc44hd2ZiY4njcIlvKERzIHflcoUh\XiycnXzd4lwdiCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG2IEGwNEB2VSCjZoTldkA1QCCqcoO= Mme5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-231 NIHOOIJCeG:ydH;zbZMh[XO|YYm= NWLaR2RGOTByIIXN M2XC[lI1KGi{cx?= NUXwfopSUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBUfGG2MzDTTFIh\G:vYXnuJJRz[W6|ZnXjeIVlKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZZQhOTByIIXNJIFnfGW{IEK0JIhzew>? Mn25QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-231 NFy5[pBCeG:ydH;zbZMh[XO|YYm= M3v0TlExOCC3TR?= NXXGdIU1OjRiaILz M4n0OWlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gV5RifDOFIITyZY5{\mWldHXkJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDJ2IHjydy=> M2XMU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
NIH3T3 MULGeY5kfGmxbjDhd5NigQ>? MnPLNVAxKHWP MVm0PEBpenN? NYnSd3BbWmWmdXP0bY9vKG:oIHP5Z4xqdiCGMTDn[Y5mKGW6cILld5Nqd25iaX6gek1UemNidILhcpNn\WO2ZXSgcY92e2ViTlnIN3Q{KGOnbHzzJIF1KDFyMDD1UUBi\nSncjC0PEBpenN? NXryZ4lFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
NIH3T3 MmTPSpVv[3Srb36gZZN{[Xl? NXLwW3hnOTByIIXN NXvCVFMxPDhiaILz MnLEVoVlfWO2aX;uJI9nKEKlbD34UEBo\W6nIHX4dJJme3Orb36gbY4hfi2VcnOgeJJidnOoZXP0[YQhdW:3c3WgUmlJO1R|IHPlcIx{KGG2IEGwNEB2VSCjZoTldkA1QCCqcoO= NH3LNGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
NIH3T3 M2nRNmZ2dmO2aX;uJIF{e2G7 NHTxd2kyODBidV2= NVq4e4FOPDhiaILz NVzUdI5sWmWmdXP0bY9vKG:oIIP1dpZqfmmwIHflcoUh\XiycnXzd4lwdiCrbjD2MXNz[yC2cnHud4Zm[3SnZDDtc5V{\SCQSVizWFMh[2WubIOgZZQhOTByIIXNJIFnfGW{IES4JIhzew>? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-231 NHW2OlRHfW6ldHnvckBie3OjeR?= MnHkNVAxKHWP NHPFNXM1QCCqcoO= NVzp[lVnWmWmdXP0bY9vKG:oIFLjcE15VCCpZX7lJIV5eHKnc4Ppc44hcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BifCBzMECgeW0h[W[2ZYKgOFghcHK| M3;FdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
MDA-MB-231 MoPWSpVv[3Srb36gZZN{[Xl? NEXReW8yODBidV2= MnfuOFghcHK| MV7S[YR2[3Srb36gc4Yhe3W{dnn2bY4h\2WwZTDlfJBz\XO|aX;uJIlvKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZZQhOTByIIXNJIFnfGW{IES4JIhzew>? M3SyO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
MDA-MB-231 M{izT2FvfGm2dX3vdkBie3OjeR?= MX21JI1oN2up MUKyJJdm\Wu| M{faU2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiZYjwdoV{e2mwZzDhZ5RqfmViU4TheFMhgGWwb3fyZYZ1\WRiaX6gcY92e2ViYYSgOUBu\y:tZzygbZYh\m:{IHX2[ZJ6KDNiZHH5d{Bnd3JiMjD3[YVsew>? NF\m[Xo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
NIH3T3 NYK0RlhmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M2DKblExOCC3TR?= MnryS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJO1R|IHPlcIx{KGW6cILld5Nqdmdidj3TdoMh[XRiMUCwJJVOKG[xcjDleoVzgSB|IHThfZMh[nlic3;meE1i\2G{IHPvcI9vgS2ob4LtZZRqd25iYYPzZZk> NEm5WXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
A673 NFm2dW9yUFSVIHHzd4F6 MlW5dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEF4N{OgZ4VtdHN? NWnGSHBXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC MXvxTHRUKGG|c3H5 MYLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= Mnu1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 NX7JXFlEeUiWUzDhd5NigQ>? NUfr[nZUeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsdy=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
LAN-5 MW\xTHRUKGG|c3H5 M3:wVpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCOQV6tOUBk\Wyucx?= NVrsU2x[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
STAT3 / p-STAT3 / Cyclin D1 / Bcl-2 / Nanog / OCT4 / ALDH1 / CD44; 

PubMed: 26556875     


Western blotting shows S3I-201 treatment decrease Cyclin D1, Bcl2 and self-renewal marker Nanog, OCT4, ALDH1, and CD44 in a dose dependent manner. Data shown are representative of three individual experiments.

PD-L1; 

PubMed: 28605599     


The expression level of PD-L1 was measured by Western blotting in a head and neck squamous cell carcinoma cell line (Cal 27 and FaDu), with and without S3I-201. β-Actin was used as the internal protein loading control. PD-L1, programmed death 1 ligand; STAT, signal transducers and activators of transcription; p-STAT3, phosphorylated STAT3.

26556875 28605599
Immunofluorescence
p-STAT3; 

PubMed: 26556875     


Immunoflurosece shows S3I-201 reduce nuclear expression of p-STAT3Tyr705 by confocal microscope. 

Oct4 / Twist ; 

PubMed: 27521216     


Suppression of Oct4 and Twist expression was shown in TW01 CD44/CD24 co-overexpressed cells and CSCs after S3I-201 treatment. Scale bars indicate 20 μm.

26556875 27521216
Growth inhibition assay
Cell viability; 

PubMed: 26813676     


The STAT3 inhibitor (S3I-201) decreases cellular proliferation in HTLV-I/ATL-lines. Cells were treated with 0, 12.5, 25, or 50 μM S3I-201 or dimethyl sulfoxide (DMSO) for 72 hours. The average growth curve is representative of the percentage of proliferation between S3I-201 and DMSO-treated cells. Each cell line was treated at least twice for standard deviation. 1185 (-IL2) cells were washed in phosphate-buffered saline and resuspended in media without IL2, followed by treatment with S3I-201 for 72 hours.

26813676
体内研究 5 mg/kg剂量的S3I-201作用于携带人类乳腺癌(MDA-MB-231)的鼠时,显示出强抑制效果。[1]5 mg/kg剂量的S3I-201作用于Huh-7移植瘤,显示出强抗癌活性,没有明显的外部健康改变或者体重减轻症状。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

体外Stat3 DNA结合实验和EMSA分析:

100 mL生物素-e-Ac-EPQpYEEIEL-OH (溶于50 mM Tris/150 mM NaCl, pH 为7.5) 加到链霉亲和素包被的96孔微型板的每孔中,在4oC下振荡温育过夜。用PBS/Tween-20冲洗板,然后加入2次200 mL BSA-T-PBS (0.2% BSA/0.1% Tween-20/PBS)。50 mL Lck-SH2-GST 融合蛋白(6.4 ng/ml,溶于BSA-T-PBS中)加到96孔板的每孔中,在室温下振荡处理4小时。转移溶液,用200 mL BSA-T-PBS冲洗4次,然后在每孔中加入100 mL多克隆GST抗体 (100 ng/mL,溶于BSA-T-PBS),4oC下温育过夜。用BSA-T-PBS冲洗后, 在每孔中加入100 mL 200 ng/ml BSA-T-PBS辣根过氧化物酶结合的二抗,然后在室温下温育45分钟。用BSA-T-PBS冲洗4步后,再用PBS-T冲洗3步,然后在每孔中加入100 mL过氧化物酶底物,温育5到15分钟。加入100 mL 1 M硫酸溶液终止过氧化物酶反应,使用ELISA计数板在450nm 处读取吸光值。
细胞实验:[2]
- 合并
  • Cell lines: HCC细胞系包括HepG2, PLC/PRF/5, SNU-449, Huh-7, SNU-398, SNU-182,和SNU-475
  • Concentrations: 25到250 μM
  • Incubation Time: 72小时
  • Method: 5 ×103个细胞接种在含完全培养基的96孔微型板上。培养72小时后,每孔加入100 μL 2 mg/mL MTT溶液测量存活细胞数。2小时后,转移培养基,每孔加入100 μL二甲亚砜溶解甲结晶。使用酶联免疫吸附法计数器在590 nm处读取吸光值。
    (Only for Reference)
动物实验:[1]
- 合并
  • Animal Models: 6周大的携带MDA-MB-231移植瘤模型的雌性无胸腺裸鼠
  • Dosages: 5 mg/kg
  • Administration: 静脉注射,每周2天或3天。
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 73 mg/mL (199.8 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+corn oil
3mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 365.36
化学式

C16H15NO7S

CAS号 501919-59-1
储存条件 粉状
溶于溶剂
别名 NSC 74859

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID