S3I-201

For research use only. Not for use in humans.

目录号:S1155 别名: NSC 74859

S3I-201 Chemical Structure

CAS No. 501919-59-1

S3I-201 (NSC 74859) 有效抑制STAT3的DNA结合活性,IC50为86 μM,而对STAT1和STAT5的抑制活性低。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 638.93 现货
RMB 563.02 现货
RMB 972.36 现货
RMB 3028.28 现货
RMB 8165.43 现货
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客户使用Selleck生产的S3I-201发表文献159篇:

产品安全说明书

STAT抑制剂选择性比较

生物活性

产品描述 S3I-201 (NSC 74859) 有效抑制STAT3的DNA结合活性,IC50为86 μM,而对STAT1和STAT5的抑制活性低。
特性 S3I-201是化学探测器抑制剂,可以除去乳腺瘤。
靶点
STAT3 [1]
(Cell-free assay)
86 μM
体外研究

S3I-201选择性抑制Stat3 DNA结合活性。S3I-201抑制Stat3·Stat3复合形式,不依赖于Stat3的激活状态。S3I-201不干涉Lck SH2与同源pTyr肽结合。S3I-201作用于NIH 3T3/v-Src鼠成纤维细胞和人类乳腺癌MDA-MB-231, MDA-MB-435, 和MDA-MB-468 细胞,可以抑制Stat3激活。S3I-201 抑制Stat3依赖的转录活性。 S3I-201 也抑制编码cyclin D1, Bcl-xL,和surviving 的Stat3-调节基因的表达。[1]S3I-201可以降低 pS727STAT3水平和降低TGF-β通路蛋白水平。S3I-201也可抑制CD133+ 和CD133− Huh-7细胞。[2]最新研究显示S3I-201作用于Hep-G2, Huh-7和SK-HEP-1细胞,加强 Cetuximab的抗增殖效果。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
U87 Ml24S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXwO|IhcA>? MkDzTWM2OD13NT6xJO69VQ>? NGDMTZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC3NlY2Oid-MkCwO|I3PTJ:L3G+
U373  M1rF[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjSUFE6PzJiaB?= MoWzTWM2OD13Mj61JO69VQ>? M2W3PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEeyOlUzLz5{MEC3NlY2OjxxYU6=
HPAC M3fSVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVK3NkBp NEPud5pKSzVy78{eNVAxKM7:TR?= M2LPbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEeyOlUzLz5{MEC3NlY2OjxxYU6=
PANC-1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYW3NkBp M{i3TGlEPTExvK6xNFAh|ryP NFLNT2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC3NlY2Oid-MkCwO|I3PTJ:L3G+
SK-BR-3 NWTIOIk1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFzybmk4OiCq M2m2ZmlEPTExvK6xNFAh|ryP NFrMSIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC3NlY2Oid-MkCwO|I3PTJ:L3G+
 U-373 MG NVzWS4plS3m2b4TvfIlkcXS7IFHzd4F6 MYOzM|ExKM7:TR?= NXnBRWFMOjRiaB?= M2H4N5Jm\HWlZYOgSm4u|rNvaX7keYNm\CClZXzsJI5mfXKxdH;4bYNqfHl? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh6OESxOkc,OjB6OEi0NVY9N2F-
MDA-MB-231 NF;EN5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXLNUXNSPzJiaB?= NWTrNYl4UUN3MP-8olExOCEQvF2= NGPN[5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC3NlY2Oid-MkCwO|I3PTJ:L3G+
HUVEC  NGDaXWRHfW6ldHnvckBCe3OjeR?= NVXpfmk{OC53LUKwJO69VQ>? NU\BfW1wOjRiaB?= MVXEUXNQ NIHq[Vd{fXCycnXzd4V{KHSqZTDofZBwgGmjLXnu[JVk\WRiYXPjeY12dGG2aX;uJI9nKEiLRj2x{tE> NW\IRXp{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1NlM2PTlpPkKxOVI{PTV7PD;hQi=>
Huh7 M4PKWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXwdWw6OTByIH7N NID4eVg1QCCq MkjWSG1UVw>? NGHDOIdqdmirYnn0d{B1cGViSVytOkB{fGmvdXzheIlwdiCycn;tc5Rm\CClZXzsJJBzd2yrZnXyZZRqd25? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN4NEO4PUc,OjN|NkSzPFk9N2F-
PLC/PRF/5  NITYOIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV[xNFAhdk1? NVnKOVRXPDhiaB?= NF:1WWFFVVOR MlyybY5pcWKrdIOgeIhmKEmOLU[gd5RqdXWuYYTpc44heHKxbX;0[YQh[2WubDDwdo9tcW[ncnH0bY9v MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN4NEO4PUc,OjN|NkSzPFk9N2F-
H460  M4LUXmZ2dmO2aX;uJGF{e2G7 NYHlOY9[PTBxMUCwJO69VQ>? NIfsPYk1QCCq NY\0dFRTcW6qaXLpeJMhfGinIGP0ZZQ{SyCrbnPy[YF{\WRibXnSMVkz[SCneIDy[ZN{cW:w M4S0UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEKwNlU1Lz5{M{iyNFI2PDxxYU6=
H1299 M4G3NWZ2dmO2aX;uJGF{e2G7 NV\wdHhZPTBxMUCwJO69VQ>? NX\sUVM4PDhiaB?= NV\EeGlJe3WycILld5NmeyCvaWKtPVJiKGW6cILld5Nqd25iZH;z[U1l\XCnbnTlcpRtgQ>? MmP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6MkCyOVQoRjJ|OEKwNlU1RC:jPh?=
T-cell  MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfwOnRCUUN3ME21NEDPxE1? NIjEOFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2PFc{OSd-MkSwOlg4OzF:L3G+
U373  NUD4UWtET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4fRelEzPSEQvF2= NVrmfJRzOjRiaB?= M3Ty[2ROW09? NHG2dGZlcXO{dYD0d{BUXEGWMzDzbYdv[WyrbnegZY5lKHC{b3zp[oVz[XSrb36= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB5MEiyNEc,OjRyN{C4NlA9N2F-
HUT-102 NU\pfZl{SXCxcITvd4l{KEG|c3H5 MlzaO|UuOzByIN88US=> NV3HcXdFOjRxNEigbC=> MlP4d5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZIh[W6mIHnu[JVk\XNiY3XscEBieG:ydH;zbZPDqA>? MkTqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUC5PVUoRjJ2MEmwPVk2RC:jPh?=
MT-2 NELiXlBCeG:ydH;zbZMhSXO|YYm= MVW3OU0{ODBizszN MmntNlQwPDhiaB?= NHP0UZN{fXCycnXzd4V{KGOnbHygdJJwdGmoZYLheIlwdiCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meiCjbnSgbY5lfWOnczDj[YxtKGGyb4D0c5Nqe8Li NFzlfYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5NFk6PSd-MkSwPVA6QTV:L3G+
H460  M3TNTWFxd3C2b4Ppd{BCe3OjeR?= Ml60NVAxyqCwTR?= Mn\lNlTDqGh? NXPqcmdw\W6qYX7j[ZMh[2WubDDk[YF1cCClbz30doVifGWmIIfpeIghVFl{OUSwNFI> M{TMbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEeyOVM5Lz5{NES3NlU{QDxxYU6=
A459 M4LiVGFxd3C2b4Ppd{BCe3OjeR?= M1LO[VExOMLibl2= NYPHOmF5OjUEoHi= MnTNbY5lfWOnczDj[YxtKGGyb4D0c5NqeyClbz30doVifGWmIIfpeIghSkWcMkO1 NVL3Z443RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|I2OzhpPkK0OFczPTN6PD;hQi=>
H460  NUC2eIhSSXCxcITvd4l{KEG|c3H5 MmjoNVAxyqCwTR?= MV:yOOKhcA>? MYnpcoR2[2W|IHPlcIwh[XCxcITvd4l{KGOxLYTy[YF1\WRid3n0bEBDTVp{M{W= NUK3d5RyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0O|I2OzhpPkK0OFczPTN6PD;hQi=>
GC  MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGe3UIo2OC1zMkWg{txO NX[0N25PPzJiaB?= NITRO3hifHSnboXheIV{KHSqZTDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MoXpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5N{S1NFMoRjJ3N{e0OVA{RC:jPh?=
GH3 M1ThSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDRe|NkPTBvMUK1JO69VQ>? NIDvc2g4OiCq NWHT[pc2[XS2ZX71ZZRmeyC2aHWgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NHjT[Ho9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe3OFUxOyd-MkW3O|Q2ODN:L3G+
BT474R  NFfZ[nJHfW6ldHnvckBCe3OjeR?= NF\aNVQ2OCEQvF2= MV:xNE03OCCm MmX2bY5pcWKrdIOgV3RCXDNiYXP0bZZqfHl? NWrtcGpXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNlc2PjFpPkK1N|I4PTZzPD;hQi=>
NCI-N87R MXzGeY5kfGmxbjDBd5NigQ>? MkLzOVAh|ryP MU[xNE03OCCm NXLzW5p{cW6qaXLpeJMhW1SDVEOgZYN1cX[rdIm= NV3LR|JWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNlc2PjFpPkK1N|I4PTZzPD;hQi=>
MDA-MB-468 M1e1XmZ2dmO2aX;uJIF{e2G7 NF3OfYIyODBidV2= NWLNNYJVOjRiaILz MljqTY5pcWKrdHnvckBw\iCVdHH0N{Bi[3SrdnH0bY9vKGmwIHj1cYFvKE2GQT3NRk01PjhiY3XscJMh[XRiMUCwJJVOKGGodHXyJFI1KGi{cx?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-435 NIrqU21HfW6ldHnvckBie3OjeR?= NEfhencyODBidV2= NF;xSVYzPCCqcoO= MlLjTY5pcWKrdHnvckBw\iCVdHH0N{Bi[3SrdnH0bY9vKGmwIHj1cYFvKE2GQT3NRk01OzViY3XscJMh[XRiMUCwJJVOKGGodHXyJFI1KGi{cx?= NVruRmpVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
MDA-MB-231 NIrIXoxHfW6ldHnvckBie3OjeR?= NHX0SowyODBidV2= MoHBNlQhcHK| NFTpUlZKdmirYnn0bY9vKG:oIGP0ZZQ{KGGldHn2ZZRqd25iaX6gbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjdDCxNFAhfU1iYX\0[ZIhOjRiaILz M1HhbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
NIH3T3 M4TVTmZ2dmO2aX;uJIF{e2G7 M2rqdFExOCC3TR?= NV7ZZ3pYOjRiaILz NH;hW3VT\WS3Y4Tpc44hd2ZicGT5dk04ODViU4TheFMhdGW4ZXygbY4hfi2VcnOg[ZhxemW|c3nu[{Bud3W|ZTDOTWg{XDNiY3XscJMh[XRiMUCwJJVOKGGodHXyJFI1KGi{cx?= NH\acmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
NIH3T3 MoO4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NE\sdnkyODBidV2= M3nX[lQh\GG7cx?= M2fveWdzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJG5KUDOWMzDj[YxteyCneIDy[ZN{cW6pII[tV5JkKGG2IEGwNEB2VSCjZoTldkA1KGSjeYOgZpkhfHK7cHHuJIJtfWViZYjjcJV{cW:wIHHzd4F6 NXGzSFlXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
MDA-MB-435 M3XQeGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUGxNFAhfU1? NILRTo41KGSjeYO= MojHS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWRCNU2ELUSzOUBk\WyuczDlfJBz\XO|aX7nJJYuW3KlIHH0JFExOCC3TTDh[pRmeiB2IHThfZMh[nlidIL5dIFvKGKudXWg[ZhkdHW|aX;uJIF{e2G7 MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
MDA-MB-231 MXzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2jTfFExOCC3TR?= MleyOEBl[Xm| MmLtS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDlfJBz\XO|aX7nJJYuW3KlIHH0JFExOCC3TTDh[pRmeiB2IHThfZMh[nlidIL5dIFvKGKudXWg[ZhkdHW|aX;uJIF{e2G7 MlP3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-468 M2q4S2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXrSeJlXOTByIIXN NX\zXJA6PCCmYYnz NWLyR3l2T3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVQ3QCClZXzsd{BmgHC{ZYPzbY5oKHZvU4LjJIF1KDFyMDD1UUBi\nSncjC0JIRigXNiYomgeJJ6eGGwIHLseYUh\XilbIXzbY9vKGG|c3H5 NHPuR5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
NIH3T3 MlyzS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYCxNFAhfU1? MoTPS5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgUmlJO1R|IHPlcIx{KGW6cILld5Nqdmdidj3SZZMh[XRiMUCwJJVOKG[xcjDleoVzgSB|IHThfZMh[nlic3;meE1i\2G{IHPvcI9vgS2ob4LtZZRqd25iYYPzZZk> NVT1epZYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
MDA-MB-435 MYnBdI9xfG:|aYOgZZN{[Xl? NYfzS5NMOzBidH:gNVAxKHWP MX60PEBpenN? MWrJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKE2GQT3NRk01OzViY3XscJMh\XiycnXzd4lv\yCjY4TpeoUhW3SjdEOgZZQhOzBidH:gNVAxKHWPIHHmeIVzKDR6IHjydy=> Ml7mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-231 M4HIbmFxd3C2b4Ppd{Bie3OjeR?= NXnBfnlPOTByIIXN NWD3PYtROjRiaILz NUXKOHgzWmWmdXP0bY9vKG:oIHHwc5B1d3OrczDpckBUfGG2MzD0doFve2[nY4Tl[EBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHH0JFExOCC3TTDh[pRmeiB{NDDodpM> NV7rZmlvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
MDA-MB-231 NV\kbYw3TnWwY4Tpc44h[XO|YYm= M13EfVExOCC3TR?= NEj0RWg1QCCqcoO= NF7wUWRT\WS3Y4Tpc44hd2ZiY4njcIlvKERzIHflcoUh\XiycnXzd4lwdiCrbjDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGG2IEGwNEB2VSCjZoTldkA1QCCqcoO= NGjqW|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-231 MlHvRZBweHSxc3nzJIF{e2G7 MVexNFAhfU1? NXrsVGx[OjRiaILz M3rp[Glv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gV5RifDNiU1iyJIRwdWGrbjD0doFve2[nY4Tl[EBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHH0JFExOCC3TTDh[pRmeiB{NDDodpM> NGDhOVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
MDA-MB-231 NFnETGtCeG:ydH;zbZMh[XO|YYm= M2HwV|ExOCC3TR?= M4HUWlI1KGi{cx?= M4LRTmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gV5RifDOFIITyZY5{\mWldHXkJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDJ2IHjydy=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
NIH3T3 NHvzNVBHfW6ldHnvckBie3OjeR?= MXGxNFAhfU1? MUe0PEBpenN? NV7ycmhIWmWmdXP0bY9vKG:oIHP5Z4xqdiCGMTDn[Y5mKGW6cILld5Nqd25iaX6gek1UemNidILhcpNn\WO2ZXSgcY92e2ViTlnIN3Q{KGOnbHzzJIF1KDFyMDD1UUBi\nSncjC0PEBpenN? MlexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
NIH3T3 NVe5OJN4TnWwY4Tpc44h[XO|YYm= NGrQfW8yODBidV2= MWS0PEBpenN? M{fQcnJm\HWldHnvckBw\iCEY3ytfGwh\2WwZTDlfJBz\XO|aX;uJIlvKHZvU4LjJJRz[W6|ZnXjeIVlKG2xdYPlJG5KUDOWMzDj[YxteyCjdDCxNFAhfU1iYX\0[ZIhPDhiaILz MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR4M{C5NEc,OTd2NkOwPVA9N2F-
NIH3T3 M3nSdmZ2dmO2aX;uJIF{e2G7 MYGxNFAhfU1? MkjvOFghcHK| Mn[3VoVlfWO2aX;uJI9nKHO3co\peolvKGenbnWg[ZhxemW|c3nvckBqdiC4LWPyZ{B1emGwc3\lZ5Rm\CCvb4Xz[UBPUUh|VEOgZ4VtdHNiYYSgNVAxKHWPIHHmeIVzKDR6IHjydy=> NUn2eWlSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OlMxQTBpPkG3OFY{ODlyPD;hQi=>
MDA-MB-231 Mn;YSpVv[3Srb36gZZN{[Xl? MXixNFAhfU1? NIfV[WM1QCCqcoO= MlTJVoVlfWO2aX;uJI9nKEKlbD34UEBo\W6nIHX4dJJme3Orb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDheEAyODBidV2gZYZ1\XJiNEigbJJ{ M4fVWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
MDA-MB-231 MnzSSpVv[3Srb36gZZN{[Xl? NXfsN295OTByIIXN M2ewSFQ5KGi{cx?= MX3S[YR2[3Srb36gc4Yhe3W{dnn2bY4h\2WwZTDlfJBz\XO|aX;uJIlvKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZZQhOTByIIXNJIFnfGW{IES4JIhzew>? Mo\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NkOwPVAoRjF5NE[zNFkxRC:jPh?=
MDA-MB-231 MojnRY51cXS3bX;yJIF{e2G7 M2PqcVUhdWdxa3e= MnS4NkB4\WWtcx?= M2PpNWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiZYjwdoV{e2mwZzDhZ5RqfmViU4TheFMhgGWwb3fyZYZ1\WRiaX6gcY92e2ViYYSgOUBu\y:tZzygbZYh\m:{IHX2[ZJ6KDNiZHH5d{Bnd3JiMjD3[YVsew>? M3m3OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NE[zNFkxLz5zN{S2N|A6ODxxYU6=
NIH3T3 NYXpc4xsT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYXoe4RVOTByIIXN NX7JNJVoT3Kxd4ToJIlvcGmkaYTpc44hd2ZibX;1d4UhVkmKM2SzJINmdGy|IHX4dJJme3Orbnegek1UemNiYYSgNVAxKHWPIH\vdkBmfmW{eTCzJIRigXNiYomgd49nfC2jZ3HyJINwdG:weT3mc5Ju[XSrb36gZZN{[Xl? NGjOWmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S2N|A6OCd-MUe0OlMxQTB:L3G+
A673 NVzRVJA4eUiWUzDhd5NigQ>? M1e4UJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy| MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC MlHMdWhVWyCjc4PhfS=> MY\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 NYG0N|FxeUiWUzDhd5NigQ>? NHflPJhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz NEjFUHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 M2DITJFJXFNiYYPzZZk> Mnf6dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| NYT1NJMxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
STAT3 / p-STAT3 / Cyclin D1 / Bcl-2 / Nanog / OCT4 / ALDH1 / CD44; 

PubMed: 26556875     


Western blotting shows S3I-201 treatment decrease Cyclin D1, Bcl2 and self-renewal marker Nanog, OCT4, ALDH1, and CD44 in a dose dependent manner. Data shown are representative of three individual experiments.

PD-L1; 

PubMed: 28605599     


The expression level of PD-L1 was measured by Western blotting in a head and neck squamous cell carcinoma cell line (Cal 27 and FaDu), with and without S3I-201. β-Actin was used as the internal protein loading control. PD-L1, programmed death 1 ligand; STAT, signal transducers and activators of transcription; p-STAT3, phosphorylated STAT3.

26556875 28605599
Immunofluorescence
p-STAT3; 

PubMed: 26556875     


Immunoflurosece shows S3I-201 reduce nuclear expression of p-STAT3Tyr705 by confocal microscope. 

Oct4 / Twist ; 

PubMed: 27521216     


Suppression of Oct4 and Twist expression was shown in TW01 CD44/CD24 co-overexpressed cells and CSCs after S3I-201 treatment. Scale bars indicate 20 μm.

26556875 27521216
Growth inhibition assay
Cell viability; 

PubMed: 26813676     


The STAT3 inhibitor (S3I-201) decreases cellular proliferation in HTLV-I/ATL-lines. Cells were treated with 0, 12.5, 25, or 50 μM S3I-201 or dimethyl sulfoxide (DMSO) for 72 hours. The average growth curve is representative of the percentage of proliferation between S3I-201 and DMSO-treated cells. Each cell line was treated at least twice for standard deviation. 1185 (-IL2) cells were washed in phosphate-buffered saline and resuspended in media without IL2, followed by treatment with S3I-201 for 72 hours.

26813676
体内研究 5 mg/kg剂量的S3I-201作用于携带人类乳腺癌(MDA-MB-231)的鼠时,显示出强抑制效果。[1]5 mg/kg剂量的S3I-201作用于Huh-7移植瘤,显示出强抗癌活性,没有明显的外部健康改变或者体重减轻症状。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

体外Stat3 DNA结合实验和EMSA分析:

100 mL生物素-e-Ac-EPQpYEEIEL-OH (溶于50 mM Tris/150 mM NaCl, pH 为7.5) 加到链霉亲和素包被的96孔微型板的每孔中,在4oC下振荡温育过夜。用PBS/Tween-20冲洗板,然后加入2次200 mL BSA-T-PBS (0.2% BSA/0.1% Tween-20/PBS)。50 mL Lck-SH2-GST 融合蛋白(6.4 ng/ml,溶于BSA-T-PBS中)加到96孔板的每孔中,在室温下振荡处理4小时。转移溶液,用200 mL BSA-T-PBS冲洗4次,然后在每孔中加入100 mL多克隆GST抗体 (100 ng/mL,溶于BSA-T-PBS),4oC下温育过夜。用BSA-T-PBS冲洗后, 在每孔中加入100 mL 200 ng/ml BSA-T-PBS辣根过氧化物酶结合的二抗,然后在室温下温育45分钟。用BSA-T-PBS冲洗4步后,再用PBS-T冲洗3步,然后在每孔中加入100 mL过氧化物酶底物,温育5到15分钟。加入100 mL 1 M硫酸溶液终止过氧化物酶反应,使用ELISA计数板在450nm 处读取吸光值。
细胞实验:[2]
- 合并
  • Cell lines: HCC细胞系包括HepG2, PLC/PRF/5, SNU-449, Huh-7, SNU-398, SNU-182,和SNU-475
  • Concentrations: 25到250 μM
  • Incubation Time: 72小时
  • Method: 5 ×103个细胞接种在含完全培养基的96孔微型板上。培养72小时后,每孔加入100 μL 2 mg/mL MTT溶液测量存活细胞数。2小时后,转移培养基,每孔加入100 μL二甲亚砜溶解甲结晶。使用酶联免疫吸附法计数器在590 nm处读取吸光值。
    (Only for Reference)
动物实验:[1]
- 合并
  • Animal Models: 6周大的携带MDA-MB-231移植瘤模型的雌性无胸腺裸鼠
  • Dosages: 5 mg/kg
  • Administration: 静脉注射,每周2天或3天。
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 73 mg/mL (199.8 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+corn oil
3mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 365.36
化学式

C16H15NO7S

CAS号 501919-59-1
储存条件 粉状
溶于溶剂
别名 NSC 74859

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID