MK-2206 2HCl

目录号:S1078

仅限科研使用

MK-2206 2HCl是一种高度选择性的Akt1/2/3抑制剂,在无细胞试验中IC50分别为8 nM/12 nM/65 nM;对250种其他蛋白激酶没有抑制活性。MK-2206 2HCl 在肿瘤细胞中可诱导自噬凋亡。Phase 2。

MK-2206 2HCl Chemical Structure

CAS: 1032350-13-2

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1789.01 现货
RMB 895.01 现货
RMB 3032.85 现货
RMB 4655.02 现货
大包装 有超大折扣
大剂量询单有大折扣!

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

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客户使用Selleck生产的MK-2206 2HCl发表文献1568篇:

产品安全说明书

Akt抑制剂选择性比较

相关Akt产品

生物活性

产品描述 MK-2206 2HCl是一种高度选择性的Akt1/2/3抑制剂,在无细胞试验中IC50分别为8 nM/12 nM/65 nM;对250种其他蛋白激酶没有抑制活性。MK-2206 2HCl 在肿瘤细胞中可诱导自噬凋亡。Phase 2。
特性 MK-2206是第一个进入临床研究阶段的Akt小分子变构抑制剂。
靶点
Akt1 [1]
(Cell-free assay)
Akt2 [1]
(Cell-free assay)
Akt3 [1]
(Cell-free assay)
8 nM 12 nM 65 nM
体外研究

MK-2206是变构抑制剂,由pleckstrin同源结构域激活。MK-2206抑制Akt的苏氨酸308位点和丝氨酸473位点的自身磷酸化作用。另外,MK-2206阻止Akt调节的下游信号分子(包括TSC2, PRAS40,及核糖体S6蛋白)的磷酸化作用。与抑制Ras 突变型细胞系(如NCI-H358, NCI-H23, NCI-H1299, 和Calu-6)相比,MK-2206 更有效地抑制Ras野生型细胞系(如A431, HCC827, 和NCI-H292)。MK-2206和细胞毒素药剂如erlotinib 和lapatinib联用作用于肺部NCI-H460肿瘤细胞或者卵巢A2780肿瘤细胞,MK-2206也显示出协同效应。[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
NCI-H69 NH7zTZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHvfFdKSzVyPUS0NU4xQDNizszN NXnifJpsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
HCC38 NVjsPIU1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3VTWM2OD12MEGuOVk{KM7:TR?= MlTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NCI-H2052 M4LiSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIfYVppKSzVyPUO5PE41PzJizszN MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
NB17 M4\iSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIHQVWJKSzVyPUO5Nk42QTZizszN M3jIb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SBC-5 NVvpXpFtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PmOmlEPTB;M{i2Mlk5PSEQvF2= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
K-562 MkW5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M37LWGlEPTB;M{izMlM3KM7:TR?= NX64bIRPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
T84 NF7XSlhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILXfXdKSzVyPUO3OE44OTJizszN NUjDbo5FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
LU-135 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvq[mhKSzVyPUO2O{4xOzVizszN M1fZ[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
TE-9 Mm[wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTN4Mz61PFkh|ryP MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
IST-SL1 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPzdXo6UUN3ME2zOFcvPDBzIN88US=> MoTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
BE-13 M1LYTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37YWGlEPTB;M{S0MlE3PyEQvF2= MlL0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NCI-H1993 M4\QRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmi4TWM2OD1|NEKuN|Y2KM7:TR?= NEjyXWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
MOLT-13 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHNXmxKSzVyPUO0Nk4{OjZizszN MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
NCI-H630 MlrsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrUeVQzUUN3ME2zN|IvOjl2IN88US=> NYTReFJ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
KP-N-YN NWLufVJxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH[zfFhKSzVyPUO0NU4yOjNizszN NGexNZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
C3A NXjsWpJzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTNzMT64NFIh|ryP MnKxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
HEL MmDQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTNyOT65PFMh|ryP NHG4ZXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
NCI-H810 NXTrUYprT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;aU|NKSzVyPUOxNE42PyEQvF2= NUjNO41vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
LU-65 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mm\2TWM2OD1|MESuN|Ih|ryP M4D1bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
L-428 NEnvfWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LscGlEPTB;Mkm3MlYyPiEQvF2= Mme5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
FADU Ml25S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTJ7Nz61NUDPxE1? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
IGR-1 NIjhenJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTJ7NT62OVkh|ryP M3;I[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
HDLM-2 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\rXW1EUUN3ME2yPVQvPDB7IN88US=> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
C8166 M3HhdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvoSIl1UUN3ME2yO|gvPTB4IN88US=> NIC5c|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
TGBC11TKB NUP5N3FHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTJ5OD6xO|gh|ryP NF7oVXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
RERF-LC-MS MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWfJR|UxRTJ5Nj6wNFch|ryP MkTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
COLO-680N MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PTSWlEPTB;MkeyMlUzPyEQvF2= NVKzcG9yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
NB6 NFPXTnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPlTWM2OD1{N{CuNUDPxE1? MmTzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
GAK NX\OZnN4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1S5OmlEPTB;MkWzMlAxOiEQvF2= MlLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
RPMI-8866 NG\2RYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;BTWM2OD1{NEmuNlch|ryP NWO3OlB2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
NCI-H2347 M4DrUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTJ2Nz63NVMh|ryP NFPJblI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
LS-1034 NYDIfllzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTJ2Nj6yOlYh|ryP NF;sbVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
SH-4 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MljTTWM2OD1{NE[uNFkh|ryP MontQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
CCRF-CEM MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTDW41KSzVyPUK0N{44QTVizszN M{f0SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
C2BBe1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjrS4pKSzVyPUKzNU46ODVizszN MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
COLO-320-HSR NYTqcWVTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHy1clZKSzVyPUKxOk4yOjVizszN NHLaVY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
KNS-42 NUnRPFFST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPCTWM2OD1{MU[uOVc1KM7:TR?= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
RCM-1 M4ixO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTJzND63OlIh|ryP M4nuU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
DBTRG-05MG NXmw[m5CT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTJyNz65NFkh|ryP M{SzW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SW982 M1rVXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIqxWphKSzVyPUKwO{46PDhizszN M2PiNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
SW1116 NIrBOVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTJyNz6wO|ch|ryP M3WxNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
HuCCT1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU\oS2Y6UUN3ME2yNFQvOjB6IN88US=> MlrjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
NCI-H650 MlW2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIr2R2RKSzVyPUKwNk4yODNizszN M1;jVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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MDA-MB-231 NIHwPYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUTJR|UxRTF5Nz61NFMh|ryP MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
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NCI-H1304 M2jiXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTF3ND62PVQh|ryP M4TBWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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BEN MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml7kTWM2OD1zNUOuPVI5KM7:TR?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
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SK-MEL-3 NVuzW3R[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTmTWM2OD1zNEeuPFc5KM7:TR?= MnLxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
OVCAR-8 M{XMNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTF2NT62N|Yh|ryP M162SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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COR-L88 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELiPG5KSzVyPUG0Nk4yPCEQvF2= M4\BTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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HCT-116 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPYN2ZKSzVyPUGzO{4zOTdizszN M4H4ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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NCI-H2087 Mn7iS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4fIO2lEPTB;MUOxMlQ5OyEQvF2= M3viS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
NCI-H1651 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnCTWM2OD1zM{GuNlQ{KM7:TR?= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
A2780 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXnTWM2OD1zMkiuPVg5KM7:TR?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
BALL-1 NUL4ZmJjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4X5WWlEPTB;MUK2MlI{KM7:TR?= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
COLO-679 NEjMTo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfMRnA6UUN3ME2xNlYvPzV|IN88US=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
SF268 M2nrUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTF{Nj6xOVgh|ryP MmXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
SN12C NVXuWowzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTpNmpKSzVyPUGyOE4yPzZizszN NXTTfJd4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
NCI-H1770 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrROY56UUN3ME2xNlUvPTF2IN88US=> M1n2ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
NCI-H2452 M2jtTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTF{Mj6yNUDPxE1? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
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SW1990 NWXVRZZsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHH1WllKSzVyPUGyNE46PTFizszN MoDqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
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GOTO MoHES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInyWFJKSzVyPUGwO{44PzdizszN M17UN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
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SBC-1 MlPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\QVoRCUUN3ME2wMlQ1ODN3IN88US=> MlrEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA4QTF5NT:nQnNCVkeHUkyvZV4>
EFM-19 MlPQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3q1[GlEPTB;MD60OFAyKM7:TR?= NVLhVoxxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
H9 Mkn1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTBwM{i4PFMh|ryP NXfPb2xbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
NCI-H720 Mlf5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2PyPGlEPTB;MD6zO|Y4QSEQvF2= NUHFN3FLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
KARPAS-45 NUX3NG9XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXQR5RlUUN3ME2wMlM4OzJzIN88US=> NH;DcY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
RS4-11 M4Hjcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M37ZRmlEPTB;MD6zOFYyKM7:TR?= NVPlNVg{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
ES5 NFfpVHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHkTJVKSzVyPUCuN|Q1PTVizszN NWTJNnRpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
MOLT-16 NHSyNZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nVbmlEPTB;MD6zNFMzKM7:TR?= M{TZVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
HH NGPnOHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvoTWM2OD1yLkOwNlg{KM7:TR?= NWfKN2JYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB5OUG3OU8oRlODTlfFVlww[T5?
T47D NEn1V2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mor0TWM2OD1yLkK4NlUh|ryP NHnYT3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
AsPC-1 NEnBV|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGDLW2ZKSzVyPUCuNlIyOjJizszN M4q4SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
MDA-MB-175-VII MnHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGKwZ3RKSzVyPUCuNVM4OzhizszN NITPbmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODd7MUe1M{c,W0GQR1XSQE9iRg>?
P12-ICHIKAWA M2iyT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV;JR|UxRTBwMUG2NkDPxE1? M3zhZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExPzlzN{WvK|5USU6JRWK8M4E,
NEC8 Mn\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPlTWM2OD1yLkC5OlUyKM7:TR?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFc6OTd3Lze+V2FPT0WUPD;hQi=>
HONE-1 NXjEc2tXTnWwY4Tpc44hSXO|YYm= M3vQO|ExKM7:TR?= M1\KUFQ5KGh? MWHEUXNQ MoHwTY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dDDheEBIOQ>? MoOwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|M{[5NlUoRjJ3M{O2PVI2RC:jPh?=
CNE-2 MkfRSpVv[3Srb36gRZN{[Xl? MoHNNVAh|ryP NFWwN5c1QCCq M2XZUmROW09? MmfrTY5lfWOnczDj[YxtKGO7Y3zlJIFzemW|dDDheEBIOQ>? NGjpbGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOzOlkzPSd-MkWzN|Y6OjV:L3G+
HONE-1 NFfNbVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVKxNEDPxE1? M{j1SFk3KGh? NHzWbGJFVVOR NVLxfZp{UUN3ME2zMlM4KM7:TR?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN|NkmyOUc,OjV|M{[5NlU9N2F-
SUNE-1 NWfhcGdmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVyxNEDPxE1? NGfDZ3M6PiCq MlnESG1UVw>? M3nV[WlEPTB;MD61NkDPxE1? Ml:xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|M{[5NlUoRjJ3M{O2PVI2RC:jPh?=
CNE-2 M3TiRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3XOOVExKM7:TR?= NFn3fJA6PiCq Mk\1SG1UVw>? MYjJR|UxRTRwNUOg{txO M2HSZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{O2PVI2Lz5{NUOzOlkzPTxxYU6=
CNE-1 NUnQT4p7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzxUXVXOTBizszN NG\TcoY6PiCq Ml;USG1UVw>? NEfO[GJKSzVyPUKuPVYh|ryP MlnmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV|M{[5NlUoRjJ3M{O2PVI2RC:jPh?=
GEO M3rVXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M13ReVUxOCCwTR?= M3fWTFczKGh? NFPxZ4xFVVOR NYnGfZZOUW6qaXLpeJMh[2WubDDndo94fGh? NWTVPVk4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1PFEzOzFpPkK0OVgyOjNzPD;hQi=>
HepG2 cell NFLuUoFCeG:ydH;zbZMhSXO|YYm= MU[yNEDPxE1? MV:yOEBp NY[zPYo{TE2VTx?= NVLFbGYzUW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= NWrJSJlHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PVc{OTlpPkKzO|k4OzF7PD;hQi=>
HepG2 cell M4i4Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGr1eII{OCEQvF2= NUiwVVB2OjRiaB?= MnPJSG1UVw>? NWLZcVFxUW6qaXLpeJMh[2WubDDndo94fGh? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd7N{OxPUc,OjN5OUezNVk9N2F-
HepG2 cell MV7LbY5ie2ViQYPzZZk> NFLWVmszOCEQvF2= M{XPRVI1KGh? MVTEUXNQ NVG2[Xo1TG:5boLl[5Vt[XSnczD0bIUheGixc4Doc5J6dGG2aX;uJI9nKEGNVB?= MoTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUezNVkoRjJ|N{m3N|E6RC:jPh?=
CEM-S M2exOGZ2dmO2aX;uJGF{e2G7 M2O4dVTjiIoQvF2= MkLMOEBp NI\2NotFVVOR NGT1cVFKdmO{ZXHz[ZMhfGinIHHtc5VvfCCxZjDjcIVifmWmIFzDOWEwSixiYTD3[YxtNWW|dHHicIl{cGWmIHH1eI9xcGGpeTDtZZJs\XJ? Ml7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4MUSyOFMoRjJ{NkG0NlQ{RC:jPh?=
CEM-R MWjGeY5kfGmxbjDBd5NigQ>? NUfiWVk4POLCid88US=> Mne0OEBp MWDEUXNQ MYDJcoNz\WG|ZYOgeIhmKGGvb4XueEBw\iClbHXheoVlKEyFNFGvRkwh[SC5ZXzsMYV{fGGkbHnzbIVlKGG3dH;wbIFogSCvYYLr[ZI> NVP6OJpFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVQzPDNpPkKyOlE1OjR|PD;hQi=>
MOLT-4 M3nGNGZ2dmO2aX;uJGF{e2G7 MUi05qCK|ryP MVO0JIg> MVvEUXNQ NV;reHlDUW6lcnXhd4V{KHSqZTDhcY92dnRib3[gZ4xm[X[nZDDMR|NCN0JuIHGge4VtdC2nc4ThZoxqe2inZDDheZRweGijZ4mgcYFzc2W{ NWDs[2R4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVQzPDNpPkKyOlE1OjR|PD;hQi=>
MOLT-4 NGLqWHpHfW6ldHnvckBCe3OjeR?= M17tXFExKM7:TR?= M{XTOlI1KGh? M{\zXmROW09? NFv0dIJDdG:la4OgZ4VtdHNiaX6geIhmKEdyL1exJJBp[XOnIH;mJJRp\SClZXzsJIN6[2yn NWLPZ4RuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK2NVQzPDNpPkKyOlE1OjR|PD;hQi=>
CEM-S NXHIc4RXS3m2b4TvfIlkKEG|c3H5 M{GxWVExKM7:TR?= MnXQOFghcA>? MVfEUXNQ M1rFVmlEPTB;NT6x5qCK|ryP MojYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4MUSyOFMoRjJ{NkG0NlQ{RC:jPh?=
CEM-R M3H2UmN6fG:2b4jpZ{BCe3OjeR?= MVqxNEDPxE1? M1ribFQ5KGh? NGDQd5lFVVOR MmDPTWM2OD1|LkRihKnPxE1? M2W3N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NkG0NlQ{Lz5{Mk[xOFI1OzxxYU6=
MOLT-4 MkfuR5l1d3SxeHnjJGF{e2G7 MWexNEDPxE1? NXfoNWd3PDhiaB?= MX7EUXNQ MnXpTWM2OD1zLkhihKnPxE1? MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZzNEK0N{c,OjJ4MUSyOFM9N2F-
HC11 NYrWVXVTTnWwY4Tpc44hSXO|YYm= NFzQR5gyOCEQvF2= M{XMWlI1KGh? NFK2c49FVVOR MmnjTY5pcWKrdIOg{tIu[2G|ZXnuJIFv\CCDRGLQJJN6dnSqZYPpdy=> MnjUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2Mk[2NlEoRjJ{NEK2OlIyRC:jPh?=
T98G NHzCSGFCeG:ydH;zbZMhSXO|YYm= MXewMlUh|ryP M4XHclYxKGh? MmrJSG1UVw>? MonBRZVodWWwdIOgZZBweHSxZ3XubYMh\W[oZXP0d{Bw\iCpZX\peIlvcWJ? M3X1TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEW3PVE1Lz5{MkC1O|kyPDxxYU6=
TAD2 NEDCdnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1nk[lExODBizszN NE\ZTYs2KGR? M1HLVmROW09? NIXrcXNKSzVyPkGwNFAh|ryP MmDDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OEmyOlcoRjJzMki5NlY4RC:jPh?=
LN229 NEf3c2hCeG:ydH;zbZMhSXO|YYm= NEjGUmcxNjVizszN NXq2d2d7PjBiaB?= M13wPWROW09? NVTCVndySXWpbXXueJMh[XCxcITv[4VvcWNiZX\m[YN1eyCxZjDn[YZqfGmwaXK= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB3N{mxOEc,OjJyNUe5NVQ9N2F-
WRO MkG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPtWmgyODByIN88US=> NXXlZYhLPSCm MonWSG1UVw>? M{DOS2lEPTB-MUCwNEDPxE1? M2S5UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
SW1736 NVvOSm5IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYTLUWtUOTByIN88US=> NIPRSoM2KGR? MYrEUXNQ NYnPPIhuUUN3ME20O{42PiEQvF2= M4LIeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
KAT18 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH64fm0{KM7:TR?= MUG1JIQ> NELVNmJFVVOR M1vCOmlEPTB;ND62NkDPxE1? M33jfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
Hth74 M2rNXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3:xbFMh|ryP MUC1JIQ> M1P1UWROW09? NGTXVHdKSzVyPUKuNVkh|ryP Ml\RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OEmyOlcoRjJzMki5NlY4RC:jPh?=
TPC1 cells harbored RET/PTC1 rearrangement NIPMUoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHnN{DPxE1? M1[yOFUh\A>? MX7EUXNQ M4nmVmlEPTB;MD61PUDPxE1? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ6OUK2O{c,OjF{OEmyOlc9N2F-
Hth7 cells harbored NRAS (Q61R+/−) MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4C2SVMh|ryP M3;BR|Uh\A>? MkflSG1UVw>? Mmn1TWM2OD12LkWg{txO NVXaUVQ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPFkzPjdpPkKxNlg6OjZ5PD;hQi=>
C643 cells harbored HRAS (G13R+/−) MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFX5b|k{KM7:TR?= NWj2WFNRPSCm M4m2bmROW09? NFfwcZVKSzVyPUCuNlch|ryP M3[5PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
FTC133 cells harbored PTEN (allele deletion and R130+) NF3mfohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4HFelMh|ryP M4rnVlUh\A>? NVrGcYp4TE2VTx?= M2q2ZmlEPTB;MD6xPEDPxE1? NHHjSoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK4PVI3Pyd-MkGyPFkzPjd:L3G+
K1 cells harbored PIK3CA (E542K+/+) MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFu2VXI{KM7:TR?= NUfpSpBJPSCm MUTEUXNQ NIHWXWNKSzVyPUCuOVIh|ryP NU\CbVZLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPFkzPjdpPkKxNlg6OjZ5PD;hQi=>
OCUT1 cells harbored PIK3CA (H1047R+/+) M1i0ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljPN{DPxE1? MWm1JIQ> NX3lTlZkTE2VTx?= MYLJR|UxRTBwMUSg{txO M4jSVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMki5NlY4Lz5{MUK4PVI3PzxxYU6=
Sk-Hep1 Mk[wR5l1d3SxeHnjJGF{e2G7 NX;jWpRDOTBizszN NIXNcFUzPCCq MlLPSG1UVw>? M3jiTnNmdnOrdHn6[ZMhemW|aYP0ZY51KGOnbHzzJJRwKHSqZTDjfZRwfG:6aXOg[YZn\WO2IH;mJJNwemGoZX7pZi=> MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJyNUmyOUc,OjF{MEW5NlU9N2F-
HepG2 NX7KRVZDS3m2b4TvfIlkKEG|c3H5 MXGxNEDPxE1? NX3GZ2hNOjRiaB?= MmDqSG1UVw>? Mo\QV4Vve2m2aYrld{Bz\XOrc4ThcpQh[2WubIOgeI8hfGinIHP5eI91d3irYzDl[oZm[3Rib3[gd49z[W[nbnni MmDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{MEW5NlUoRjJzMkC1PVI2RC:jPh?=
A431 MWDLbY5ie2ViQYPzZZk> MXy1JO69VQ>? NEi1cWo2KGh? NWT0fnRWTE2VTx?= MoP0V5VxeHKnc4Pld{B1cGVic3nncoFtcW6pIH;mJGFsfCCjbnSgSZJs MkiyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3N{GwOlkoRjJyNUexNFY6RC:jPh?=
NCI-H292 MnrLR5l1d3SxeHnjJGF{e2G7 NVXVWmIzOyEQvF2= NYjDeHVPPzJiaB?= NHvuN3ZFVVOR NVqwT29kUW6qaXLpeIlwdiCxZjDj[YxtKHC{b3zp[oVz[XSrb36= MkXIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB3N{GwOlkoRjJyNUexNFY6RC:jPh?=
HeLa MljYSpVv[3Srb36gZZN{[Xl? M{ewO|EhcA>? NFna[2VKSzVyIE2gNE4xOjJ6IN88US=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTFyMEezN{c,OjlzMEC3N|M9N2F-
NCI-H460 NE[0[oZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEDxbIU4OiCq NEDubVVKSzVyIE2gOU41KM7:TR?= NWr6emMxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA5PjJpPkK0PVAxQDZ{PD;hQi=>
HCT116 NVixZVl3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEXa[pA4OiCq NELm[FJKSzVyIE2gO{42KM7:TR?= NXj1NIl6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA5PjJpPkK0PVAxQDZ{PD;hQi=>
A673 NYjacFA5TnWwY4Tpc44h[XO|YYm= MnLIdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEF4N{OgZ4VtdHN? MkW0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
DAOY NWXlRnh1TnWwY4Tpc44h[XO|YYm= NFPL[nVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Saos-2 Mlz0SpVv[3Srb36gZZN{[Xl? NIXrNVhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU3Hvd{0zKGOnbHzz M3vhOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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RD M4PRTmZ2dmO2aX;uJIF{e2G7 NWL0[mdKeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLEJINmdGy| NWWwToRDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-SH NILmWFZHfW6ldHnvckBie3OjeR?= NFnDcJVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1UUCClZXzsdy=> NFHmWZQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 MY\GeY5kfGmxbjDhd5NigQ>? NFy0RoJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO= NHHE[ow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) NIi0NppHfW6ldHnvckBie3OjeR?= M1PTTpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucx?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 MonLSpVv[3Srb36gZZN{[Xl? NHG5WHdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz NEPxZZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 NUfoUZVjTnWwY4Tpc44h[XO|YYm= MWjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| NG\KdY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh41 MVrGeY5kfGmxbjDhd5NigQ>? MknSdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKqNEGgZ4VtdHN? Mn\xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh30 M4XodWZ2dmO2aX;uJIF{e2G7 NFLWSYtyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnizNEBk\Wyucx?= MlfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 MmXySpVv[3Srb36gZZN{[Xl? M{ToVZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz MlvkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC MlH1SpVv[3Srb36gZZN{[Xl? NYT2N5NneUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> MlnHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 MlTtSpVv[3Srb36gZZN{[Xl? MnTpdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= NIC4c3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 M4rBbWZ2dmO2aX;uJIF{e2G7 MmXIdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| M1\DRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 NHzzZWlHfW6ldHnvckBie3OjeR?= NHS1SHZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnixPEBk\Wyucx?= NV\rfox1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Assay
Methods Test Index PMID
Western blot p-AKT(T308) / p-AKT(S473) / pGSK3β(S9) / pFOXO1 / pPRAS(T246) / pBAD / pS6K(T389) / p4E-BP1 22932669
Growth inhibition assay Cell viability 24665203
Immunofluorescence LC3 CFTR/LC3 HMGA2 24490764 28794469 24476133
ELISA VEGFA 29449553
体内研究 MK-2206和这些细胞毒素药剂联用作用于NCI-H292移植瘤,MK-2206显示出非常有效的抗癌活性。卵巢癌A2780移植瘤中,按动物体重,每千克处理240mg MK-2206,则可抑制70%以上的Akt1/2在苏氨酸308位点和丝氨酸473位点的磷酸化,导致肿瘤生长抑制率达到60%。[1]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[3]

  • Akt激酶实验:

    通过得到的GSK 生物素肽段基质测定Akt激酶。使用含镧系金属螯合物偶联单抗的均相时间分辨荧光(HTRF)测定肽段磷酸化程度,这种单抗专门作用于与链霉亲和素——藻蓝素(SA-APC)载体相结合的磷酸化作用,SA-APC载体可以结合到肽段的部分生物素上。当镧系金属螯合物与藻蓝素相接触时,无辐射的能量从镧系金属螯合物传递给藻蓝素,然后藻蓝素发射波值为655纳米的光线。工作液:100X 蛋白酶抑制剂混合物(PIC):1mg/ml苯甲脒,0.5 mg/ml 抑肽素, 0.5 mg/ml亮抑肽酶, 0.5 mg/ml 抑肽酶。10X实验 buffer: 500 mM HEPES(pH 为7.5), 1% PEG, 16.6 mM EDTA, 1 mM EGTA, 1% BSA, 20 mM 9-甘油磷酸。抑制 buffer:50 mM HEPES(pH为7.3), 16.6 mM EDTA, 0.1% BSA, 0.1% Triton X-100, 0.17 nM 标记的单抗, 0.0067mg/ml SA-APC。ATP/MgCl2工作液: 1X 实验buffer, 1 mM DTT, 1X PIC, 5% 甘油, 激活的Akt。肽段工作液:1X实验buffer, 1 mM DTT, 1X PIC, 5% 甘油 2 TM GSK生物素肽段。工作液加到合适的孔中,然后加入总负荷为16的 ATP/MgCl2,反应开始。含MK-2206的实验组和对照组加入总负荷为10的肽段工作液。然后加入总负荷为13的酶液,混匀。反应进行50分钟,然后加入总负荷为60的均相时间分辨荧光抑制buffer停止反应。阻断的反应在室温下温育30分钟以上,然后使用仪器读数。

细胞实验:

[2]

  • Cell lines: A431, HCC827, NCI-H292, NCI-H358, NCI-H23, NCI-H1299, Calu-6,和NCI-H460细胞
  • Concentrations: 0, 0.3, 1,和3 μM
  • Incubation Time: 72或96小时
  • Method:

    MK-2206溶解在DMSO中,在实验使用前用培养基稀释。细胞按2~3×103密度接种在96孔板上,温育24小时。MK-2206 (分为0, 0.3, 1,和3 μmol四组)处理细胞,72或者96小时后,测定细胞增殖情况。

动物实验:

[2]

  • Animal Models: 携带SK-OV-3, NCI-H292, HCC70, PC-3,和NCI-H460模型的雄性CD1裸鼠
  • Dosages: 120 mg/kg
  • Administration: 口服处理

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+40% PEG 300+5%Tween80+ 50%H2O

0.625 mg/ml

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 480.39
化学式

C25H21N5O.2HCl

CAS号 1032350-13-2
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01480154 Active not recruiting Drug: Akt Inhibitor MK2206|Drug: Hydroxychloroquine Advanced Malignant Solid Neoplasm|Stage III Cutaneous Melanoma AJCC v7|Stage III Prostate Cancer AJCC v7|Stage III Renal Cell Cancer AJCC v7|Stage IIIA Cutaneous Melanoma AJCC v7|Stage IIIB Cutaneous Melanoma AJCC v7|Stage IIIC Cutaneous Melanoma AJCC v7|Stage IV Cutaneous Melanoma AJCC v6 and v7|Stage IV Prostate Cancer AJCC v7|Stage IV Renal Cell Cancer AJCC v7 National Cancer Institute (NCI) November 23 2011 Phase 1
NCT01466868 Terminated Drug: MK2206 Diffuse Large B Cell Lymphoma Centre Leon Berard|National Cancer Institute France November 2011 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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常见问题及建议解决方法

问题 1:
What is your recommendation for preparing the solution and how would you deliver it to the mice (i.p. or gavage)?

回答:
You can resuspend MK-2206 in 15% Captisol at up to 17mg/ml. It's a suspension and is for oral gavage use only.

问题 2:
I would want to know if MK-2206 could cross the blood brain barrier?

回答:
MK-2206 can cross BBB based on the following reference: https://clinicaltrials.gov/ct2/show/NCT01249105.