Triciribine (NSC 154020)

目录号:S1117 别名: VD-0002, vqd-002, API-2, TCN 中文名称:曲西立滨

仅限科研使用

Triciribine (NSC 154020, VD-0002, vqd-002, API-2, TCN) 是一种DNA synthesis抑制剂,也抑制PC3细胞系中的Akt和CEM-SS,H9,H9IIIB,U1细胞中的HIV-1IC50分别为130 nM和20 nM;对PI3K/PDK1没有抑制作用;作用于缺乏腺苷激酶的细胞,活性降低5000倍。Phase 1/2。

Triciribine (NSC 154020) Chemical Structure

CAS: 35943-35-2

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1235.93 现货
RMB 984.87 现货
RMB 4673.78 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

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客户使用Selleck生产的Triciribine (NSC 154020)发表文献36篇:

产品安全说明书

Akt抑制剂选择性比较

相关Akt产品

生物活性

产品描述 Triciribine (NSC 154020, VD-0002, vqd-002, API-2, TCN) 是一种DNA synthesis抑制剂,也抑制PC3细胞系中的Akt和CEM-SS,H9,H9IIIB,U1细胞中的HIV-1IC50分别为130 nM和20 nM;对PI3K/PDK1没有抑制作用;作用于缺乏腺苷激酶的细胞,活性降低5000倍。Phase 1/2。
靶点
HIV-1 [2]
(CEM-SS, H9, H9IIIB, U1 cells)
Akt [1]
(PC3 cells)
20 nM 130 nM
体外研究

Triciribine的剂量为1-10 μM时生长抑制的效果最佳。像dosnstream p70S6K一样Triciribine基本在100μM (IC50 = 130 nM)时可以抑制Akt的磷酸化作用。Triciribine抑制Nf1和Trp53突变的肿瘤星型细胞的生长,这种抑制具有梯度依赖的特性。Triciribine在GI50为1.7 μM时并不能完全抑制(最高可抑制率为69%)WHO II K1861-10系的生长,而对于更高级的肿瘤系(KR158, KR130, and SF295), Triciribine可以在更低的GI50值(0.4–1.1 mM)时产生更广泛的抑制(最高抑制率>80%)。重要的是Triciribine能特异性地抑制肿瘤细胞而对大多数普通的星形胶质细胞抑制效率很低(GI50¼13.6 mM) [1]。Triciribine能抑制HIV-1, IC50为20 nM。用量为0.1μM时能产生>90%的抑制率,用量为5μM时能完全抑制多核体的形成。 在相同的细胞系中相关的细胞毒性实验结果显示当 Triciribine 浓度为46 μM时的选择性指数为2250. 利用HIV-1急性感染的CEM-SS, h9, 和 持续感染的H9III B 和 U1 细胞研究发现, Triciribine 可以显著的抑制HIV-1诱导的 p24 核心抗原的产生, 反转录酶和传染性病毒的产生,这种抑制具有剂量依赖的特性[2]。 Triciribine会抑制人前列腺癌PC-3 细胞系中的Akt 308位的酸酸和473位丝氨酸的磷酸化 以及Akt 的活性. Triciribine使PC-3 细胞对于TRAIL- 和 anti-CD95诱导的细胞凋亡更加敏感, 但是对于能引起DNA损伤的化疗依然具有抗性[3]。Triciribine 对于Akt具有高度的选择性,并不会抑制其它激酶的激活,如磷脂酰肌醇3激酶, 磷酸肌醇依赖的激酶1, 蛋白激酶C, serumand 糖皮质激素诱导激酶, 蛋白激酶 A, 信号传感器 和转录3 活化剂, 胞外信号调节激酶1/2或者c-Jun NH2-末端 激酶[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 NX2ye481TnWwY4Tpc44h[XO|YYm= NFjJR3JV\XO2ZXSgbY4hfmm2cn:g[o9zKGO7dH;0c5hq[2m2eTDh[4FqdnO2IH31dolv\SCOMUKxNEBt\XWtZX3pZ{Bk\WyuczygTWM2OD1yLkCzOe69VQ>? M3nZfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOEiyN|cyLz5zMEi4NlM4OTxxYU6=
HFF M{DGZ2Z2dmO2aX;uJIF{e2G7 Mk[zTGNOXiCybHHxeYUh[XO|YYmge4F{KHCncn\vdo1m\CC3c3nu[{BJTkZiY3XscJMh[W6mIHXm[oVkfCC5YYOgZ4Ft[3WuYYTl[EBieyCjIIDldoNmdnSjZ3Wgc4YhemWmdXP0bY9vKGmwIH71cYJmeiCxZjDwcIFyfWW|LDDJR|UxRTJwNd88US=> NX;zdmc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC4PFI{PzFpPkGwPFgzOzdzPD;hQi=>
BSC-1 NHjmbnRCdnSrdnnyZYwh[XO|YYm= MnvFRY51cX[rcnHsJIFkfGm4aYT5JJdieyC2ZYP0[YQhfXOrbnegZY4h\W68eX3lMYxqdmunZDDpcY12dm:|b4Li[Y51KGG|c3H5JEhGVEmVQTmgeI8h\GW2ZXP0JGhUXi1zIDjo[ZJx\XNic3ntdIxmgCC4aYL1d{B1gXCnIEGpJJV{cW6pIFLTR{0yKGOnbHzzMEBKSzVyPUKz{txO MkHMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB6OEKzO|EoRjFyOEiyN|cyRC:jPh?=
HFF NHvJbIRCdnSrdnnyZYwh[XO|YYm= M3XSXGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhEVVZid3HzJIRmfGW{bXnu[YQh[nlicHzhdZVmKHKnZIXjeIlwdiCjc4PhfUB2e2mwZzDISmYh[2WubIOsJGlEPTB;Mj61{txO MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODh6MkO3N{c,OTB6OEKzO|M9N2F-
AA2 MlHJSpVv[3Srb36gZZN{[Xl? MmLIOUBpenN? MWDJcpRz[WOnbHz1cIFzKHCqb4PwbI9zgWyjdHnvckApOTByIIXNLUBqdiC3bnnu[oVkfGWmIFHBNkBk\WyuczD3ZZMhe3S3ZHnl[EBi\nSncjC1JIhzeyCxZjDJcoN2[mG2aX;uMkwhS2:wY3XueJJifGmxbk25{txO NGmxV4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEi4NlM4Oyd-MUC4PFI{PzN:L3G+
BSC-1 NVe4bnoxSW62aY\pdoFtKGG|c3H5 NF\BdFdCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJW1ZvMTD3ZZMh\GW2ZYLtbY5m\CC3c3nu[{BDW0NvMTDj[YxteyCkeTDhckBmdnq7bXWtcIlvc2WmIHntcZVvd3OxcnLlcpQh[XO|YYmgLGVNUVODKTygTWM2OD1{M988US=> M{nPOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFyOEiyN|c{Lz5zMEi4NlM4OzxxYU6=
Huh-7 MoflSpVv[3Srb36gZZN{[Xl? NEXyblhEd22yb4Xu[EB4[XNidHXzeIVlKG[xcjDpeJMh[WKrbHn0fUB1dyCrbnjpZol1KGincHH0bZRqeyCFII\pdoFtKFKQQTDy[ZBtcWOjdHnvckBqdiCKdXitO{Bk\WyuczCobJVu[W5iaHXwZZRwdWFiY3XscJMqNCCHQ{WwQVLPxE1? NUi0bIExRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWxO|c1PjRpPkG1NVc4PDZ2PD;hQi=>
MCF7 MoTnR5l1d3SxeHnjbZR6KGG|c3H5 MnrzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{BqdiCycnXz[Y5k\SCxZjCyNEB2VSClaHzvdo9yfWmwZTDifUBUWkJiYYPzZZktKEeLNUC9NE4xPc7:TR?= MmXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4OUG4PVQoRjF6NkmxPFk1RC:jPh?=
MCF7 M4nIU2N6fG:2b4jpZ4l1gSCjc4PhfS=> M1nj[WN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMhcW5icILld4Vv[2Vib3[gNVAhfU1iY3jsc5JweXWrbnWgZpkhW1KEIHHzd4F6NCCJSUWwQVAvPTcQvF2= NFPh[GY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[5NVg6PCd-MUi2PVE5QTR:L3G+
MDA-MB-231 Ml\nR5l1d3SxeHnjbZR6KGG|c3H5 MWrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHnuJJBz\XOnbnPlJI9nKDJyIIXNJINpdG:{b4H1bY5mKGK7IGPSRkBie3OjeTygS2k2OD1yLk[5{txO MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ7MUi5OEc,OTh4OUG4PVQ9N2F-
MCF7 M4rvSGN6fG:2b4jpZ4l1gSCjc4PhfS=> NWTxdGp6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUOINzDj[YxteyCkeTDTVmIh[XO|YYmsJGdKPTB;Mz62OO69VQ>? NUC0N5ZqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2PVE5QTRpPkG4OlkyQDl2PD;hQi=>
MDA-MB-468 NHPJVWpEgXSxdH;4bYNqfHliYYPzZZk> MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNE[4JINmdGy|IHnuJJBz\XOnbnPlJI9nKDJyIIXNJINpdG:{b4H1bY5mKGK7IGPSRkBie3OjeTygS2k2OD1zMD6yPe69VQ>? NHvlVZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[5NVg6PCd-MUi2PVE5QTR:L3G+
184B5 NFjSOWpEgXSxdH;4bYNqfHliYYPzZZk> NITuPYIzOCC3TR?= NH7sUlVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckAyQDSENTDj[YxteyCjdDCyNEB2VSClaHzvdo9yfWmwZTDifUBUWkJiYYPzZZktKEeLNUC9NVYvQTcQvF2= NXyyUVZNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2PVE5QTRpPkG4OlkyQDl2PD;hQi=>
MDA-MB-468 NEK0d3lEgXSxdH;4bYNqfHliYYPzZZk> M2jzT|ExKHWP MoPlR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWRCNU2ELUS2PEBk\WyuczDpckBxemW|ZX7j[UBw\iBzMDD1UUBkcGyxcn;xeYlv\SCkeTDTVmIh[XO|YYmsJGdKPTB;MkCuOFXPxE1? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ7MUi5OEc,OTh4OUG4PVQ9N2F-
184B5 M4fQ[mN6fG:2b4jpZ4l1gSCjc4PhfS=> MkjNNVAhfU1? Mn\hR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gNVg1SjViY3XscJMh[XRiMUCgeW0h[2iub4LvdZVqdmViYomgV3JDKGG|c3H5MEBIUTVyPUO0{txO MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ7MUi5OEc,OTh4OUG4PVQ9N2F-
MDA-MB-231 M2SyfmN6fG:2b4jpZ4l1gSCjc4PhfS=> MkOzNVAhfU1? NVixUmczS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{BqdiCycnXz[Y5k\SCxZjCxNEB2VSClaHzvdo9yfWmwZTDifUBUWkJiYYPzZZktKEeLNUC9N|fPxE1? MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ7MUi5OEc,OTh4OUG4PVQ9N2F-
184B5 MlrLR5l1d3SxeHnjbZR6KGG|c3H5 M4fSbWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJFE5PEJ3IHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD12MN88US=> NXuyeIQ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2PVE5QTRpPkG4OlkyQDl2PD;hQi=>
MDA-MB-468 NHXjRmhEgXSxdH;4bYNqfHliYYPzZZk> MX3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNE[4JINmdGy|IHL5JHNTSiCjc4PhfUwhT0l3ME20N{42O87:TR?= NH7aPXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[5NVg6PCd-MUi2PVE5QTR:L3G+
MDM M3;RWmFvfGm4aYLhcEBie3OjeR?= Ml;4OkBl[Xm| M{H3WGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGh2dWGwIHntcZVvd2SnZnnjbYVv[3lidnnyeZMhOSCDRFGgbY5n\WO2ZXSgbY4hcHWvYX6gUWROKGOnbHzzJIF{e2W|c3XkJIF{KGW6cILld5Nqd25ib3[gdFI1KGGwdHnn[Y4h[W[2ZYKgOkBl[Xm|IIDvd5Rqdm[nY4Tpc44h[nliRVzJV2EtKEmFNUC9NE4xODcQvF2= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB6NkG0PUc,OjByOE[xOFk9N2F-
ACH2 MWHDfZRwfG:6aXPpeJkh[XO|YYm= M{TDfmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGFEUDJiY3XscJMhcW6oZXP0[YQhf2m2aDDsZZRmdnRiSIXtZY4hcW2vdX7v[IVncWOrZX7jfUB3cXK3czCxJIJ6KE2WUzDhd5NigSxiQ1O1NF0xNjBzzszN NWPHXohNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCwPFYyPDlpPkKwNFg3OTR7PD;hQi=>
ACH2 NV\QPYx6S3m2b4TvfIlkcXS7IHHzd4F6 MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBR2gzKGOnbHzzJIlv\mWldHXkJJdqfGhibHH0[Y51KEi3bXHuJIludXWwb3Tl[olkcWWwY4mgeolzfXNiMTDifUBOXFNiYYPzZZkhcW5icILld4Vv[2Vib3[geJVud3JibnXjdo9{cXNiZnHjeI9zKGGucHjhMEBESzVyPUCuNFHPxE1? MkDmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByOE[xOFkoRjJyMEi2NVQ6RC:jPh?=
CEM-SS NVfaXWZkSW62aY\pdoFtKGG|c3H5 NYLZ[2UzPiCmYYnz Mm\5RY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTJVu[W5iaX3teY5w\GWoaXPp[Y5kgSC4aYL1d{AyKDOEIHnu[oVkfGWmIHnuJIh2dWGwIFPFUU1UWyClZXzsd{Bqdm[nY4Tl[EB4cXSqIECuO|ghfUxib3[geolzfXNic4TvZ4sh[XO|ZYPz[YQh[XNiZYjwdoV{e2mxbjDv[kBxOjRiYX70bYdmdiCjZoTldkA3KGSjeYOgdI9{fGmwZnXjeIlwdiCkeTDFUGlUSSxiSVO1NFwxNjBzzszN M2[2U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEi2NVQ6Lz5{MEC4OlE1QTxxYU6=
MDM MYPBcpRqfmm{YXygZZN{[Xl? MX62JIRigXN? NX7sdoVCSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUHWvYX6gbY1ufW6xZHXmbYNq\W6leTD2bZJ2eyBzIFLBUEBqdm[nY4Tl[EBqdiCqdX3hckBOTE1iY3XscJMh[XO|ZYPz[YQh[XNiZYjwdoV{e2mxbjDv[kBxOjRiYX70bYdmdiCjZoTldkA3KGSjeYOgdI9{fGmwZnXjeIlwdiCkeTDFUGlUSSxiSVO1NF0xNjBzON88US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB6NkG0PUc,OjByOE[xOFk9N2F-
H9 NF21WJRCdnSrdnnyZYwh[XO|YYm= MXPBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDIeY1idiCrbX31co9l\W[rY3nlcoN6KH[rcoXzJFEhW0tzIHnu[oVkfGWmIHnuJIh2dWGwIFi5JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidnnyZYwhWmW4ZYLz[UB1emGwc3PybZB1[XOnIHL5JHs{UF2WVGCgbY5kd3Kyb4LheIlwdiCjc4PhfUwhUUN3ME2wMlA{Ps7:TR?= NFm5e4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC4OlE1QSd-MkCwPFYyPDl:L3G+
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H9 M4\QN2N6fG:2b4jpZ4l1gSCjc4PhfS=> NHG3eZREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJQSClZXzsd{BjgSCPVGOgZZN{[XluIFnDOVA:OTZwM988US=> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB6NkG0PUc,OjByOE[xOFk9N2F-
H9 NEH3TIlEgXSxdH;4bYNqfHliYYPzZZk> Mk\1R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTFkh[2WubIOgZpkhVVSVIHHzd4F6NCCFQ{WwQVE6NjcQvF2= NH;zR|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEC4OlE1QSd-MkCwPFYyPDl:L3G+
Assay
Methods Test Index PMID
Western blot PUMA / p-FoxO3a / p-AKT 20978166
Immunofluorescence TRF2 / 53BP1 23862686
体内研究 Riciribine按照 1 mg/kg/天的剂量对裸小鼠腹腔注射,可以抑制 OVCAR3, OVCAR8 和 PANC1 肿瘤的生长, 分别使Akt过量表达了90%, 88% 和80%。 然而, Triciribine 对OVCAR5 和 COLO357细胞的生长基本没有影响[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
  • Akt磷酸化改变分析:

    待细胞生长到全板80%–90%,加入1–10 ng/mL表皮生长因子(EGF; cat #01-107)或血小板生长因子(PDGF)–AA (cat #01-309) (Upstate Biotechnology)加入或者不加 10–20 mM的 U0126 (cat #EI-282) 或 LY-294002 (cat #ST-420) (Biomol Research Lab)刺激细胞5到10分钟。用12%–15% SDS PAGE分离5–20 μg蛋白裂解液,用Akt (cat #cs-9272), 磷酸化的 Akt (磷酸丝氨酸 473; cat #cs-9271), MAPK (cat #cs-9102)和磷酸化 MAPK (p44/42 磷酸苏氨酸202/Tyr204; cat #cs-01-9101)抗体1:1000稀释(Cell Signaling Technology)蛋白印迹检测蛋白裂解物。

细胞实验:[2]
  • Cell lines: CEM-SS 细胞
  • Concentrations: 0-500 μM
  • Incubation Time: 48小时
  • Method: 将 CEM-SS细胞按1万个每孔的密度接种在96孔平板中并在生长培养基中培养,用上述细胞对triciribine的细胞毒性进行分析评定. 将连续稀释5倍的不同浓度的Triciribine加到细胞中. 37℃孵育48小时, 通过电转将细胞用含有放射性同位素3H的 dThd (1 μCi 每孔, 放射性比度为20 Ci/mmol)标记6小时,收取细胞并测定总的DNA合成量
动物实验:[4]
  • Animal Models: 皮下注射OVCAR3, OVCAR8, PANC1, OVCAR5和 COLO357 肿瘤细胞的80周龄雌性裸小鼠.
  • Dosages: 1 mg/kg/天
  • Administration: 腹腔注射,一天一次.

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
1% DMSO+30% polyethylene glycol+1% Tween 80

30 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 320.3
化学式

C13H16N6O4

CAS号 35943-35-2
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02987127 Unknown status -- Mucosa-Associated Lymphoid Tissue Lymphoma National Taiwan University Hospital February 2016 --
NCT00642031 Completed Drug: Triciribine Hematologic Malignancies|Leukemia Prescient Therapeutics Ltd.|VioQuest Pharmaceuticals August 2006 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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