Trastuzumab (anti-HER2)

目录号:A2007 别名: Herceptin 中文名称:曲妥珠单抗

For research use only.
Not for use in humans.

Trastuzumab (anti-HER2)曲妥珠单抗是人源重组的抗体抑制剂,与HER2的胞外区域相结合。 MW:145.53 KD。

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质量管理及产品安全说明书

HER2抑制剂选择性比较

生物活性

产品描述 Trastuzumab (anti-HER2)曲妥珠单抗是人源重组的抗体抑制剂,与HER2的胞外区域相结合。 MW:145.53 KD。
靶点
HER2 [1]
()
体外研究

Trastuzumab (anti-HER2)在过表达HER2的肿瘤细胞中发挥特异性抗肿瘤活性。Trastuzumab与HER2的近膜区结合,一旦与受体结合,这个抗体可下调HER2的表达。Trastuzumab选择性地抑制不依赖于配体的HER2-HER3二聚化。除此之外,trastuzumab与HER2结合可抑制HER2胞外区域的溶蛋白性裂解,导致p95-HER2水平降低。trastuzumab可导致PI3K信号通路和下游细胞周期进展相关介质如cyclin D1下调。Trastuzumab不仅抑制HER2信号通路,同时也在HER2过表达细胞中引起免疫相关反应。Trastuzumab的结合占用了免疫效应细胞上的Fc受体,导致抗体依赖性的细胞毒性。因此,trastuzumab具有抗血管生成的作用,降低化疗凋亡阈值[1]

体内研究 对一系列动物模型,包括灵长类动物,进行Trastuzumab的慢性给药,证明Trastuzumab是安全的[1]。在模拟亚宏观疾病的辅助治疗实验中,trastuzumab能够抑制宏观上可检测的移植瘤的生长,可持续5-7周。Trastuzumab具有显著的抗肿瘤活性,目前正用于乳腺癌的治疗中。在体内模型中,trastuzumab降低乳腺癌移植瘤的微血管密度。Trastuzumab直接作用于癌细胞信号,间接地作用于免疫系统[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:
- 合并
  • Objective: In vitro Assay of Drug Sensitivity
    Cells: JIMT-1, SKBR-3, and BT-474 cells
    Concentrations: 0, 1, 10, or 100 μg/mL
    Incubation Time: 72 h
    Method: Exponentially growing cells are harvested and plated in single wells of a 96-well flat-bottomed tissue culture plate at defined densities, ranging from 4,500-8,000 cells per well depending on the cell line. After overnight culture, the regular medium is exchanged to medium containing 0, 1, 10, or 100 μg/mL trastuzumab or trastuzumab-F(ab′)2. Cell viability is tested after 72 h of treatment. Fluorescence is detected at an excitation of 544 nm, and emission is detected at 590 nm.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/17620435

    Objective: Antibody-dependent cellular cytotoxicity (ADCC)
    Cells: Peripheral blood lymphocytes (PBLs), USPC cell lines (Uterine cancer cell)
    Concentrations: 2.5 μg/mL
    Incubation Time: --
    Method: A standard 5 h chromium (51Cr) release assay was performed to measure the cytotoxic reactivity of peripheral blood lymphocytes (PBLs) from several healthy donors in combination with trastuzumab and/or pertuzumab against USPC target cell lines.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19920829

    Ttrastuzumab can apply to immunodeficient mice (eg: SCID mice), various cancer cell lines and other related assays (Only for Reference)

动物实验:
- 合并
  • Objective: Xenograft Tumor Assays
    Animal Models: SCID C.B-17 scid/scid and nu/nmri nude mouse
    Formulation: saline
    Dosages: 5 μg/g
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/17620435

    Objective: To investigate the antitumor activity of the combination of pertuzumab and trastuzumab for gastric cancer
    Animal Models: BALB-nu/nu mice were injected (s.c.) with 5 × 106 Calu-3 cells
    Formulation: --
    Dosages: 30 mg/kg loading dose, then 15 mg/kg weekly
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19934333

    Ttrastuzumab can apply to immunodeficient mice (eg: SCID mice), various cancer cell lines and other related assays (Only for Reference)

抗体信息

CAS No. 180288-69-1
配制 PBS buffer, pH 7.2
同型 Human IgG1
来源 CHO cells
储存条件 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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常见问题及建议解决方法

  • 问题1:

    How to store the antibody?

  • 回答:

    Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

HER2 Signaling Pathway Map

相关HER2产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID