Pertuzumab (anti-HER2)

目录号:A2008 别名: Omnitarg, 2C4 中文名称:帕妥珠单抗

For research use only.
Not for use in humans.

Pertuzumab (anti-HER2)是一种人源单价克隆抗体,抑制HER2二聚化、破坏HER2与其他HER家族成员结合的能力。MW: 148 KD。

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客户使用Selleck该产品发表文献2篇:

质量管理及产品安全说明书

HER2抑制剂选择性比较

生物活性

产品描述 Pertuzumab (anti-HER2)是一种人源单价克隆抗体,抑制HER2二聚化、破坏HER2与其他HER家族成员结合的能力。MW: 148 KD。
靶点
HER2 [1]
()
体外研究

Pertuzumab (anti-HER2)显著地抑制HRG-α刺激的11_18细胞生长。Pertuzumab阻止HRG-α刺激的HER3、MAPK、Akt的磷酸化。但pertuzumab不能抑制EGF刺激的EGFR和MAPK磷酸化。免疫沉淀实验证明pertuzumab抑制HRG-α刺激的HER2/HER3异源二聚体的形成。在EGFR、HER2和HER3都过表达的PC-9细胞中,HRG-α刺激HER3磷酸化,但是HRG-α并不刺激细胞生长,也不被pertuzumab所抑制[1]。Pertuzumab与ErbB2的domain II中心结合,在空间上抑制了其结合口袋、阻止受体的二聚化和信号传递。Pertuzumab对人源ErbB2具有特异性,不与啮齿类ErbB2 (neu)以可检测到的亲和力结合[2]

体内研究 Pertuzumab (anti-HER2)在小鼠移植瘤模型中,单药给药具有抗肿瘤活性。Pertuzumab与trastuzumab结合在NCI-N87人类胃癌移植瘤模型中能够强力地减少EGFR-HER2信号,从而展现出比单药给药更为显著的抗肿瘤活性[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:
- 合并
  • Objective: Growth inhibition assay
    Cells: Human lung cancer cell lines (11_18 cells and Ma-24 cells)
    Concentrations: 0.01, 0.1, 1, 10, 100μg/mL
    Incubation Time: 72 h
    Method: The lung cells are exposed to pertuzumab (0.01-100 μg/mL) for 72 h in serum free medium with or without 100 ng/mL of epidermal growth factor (EGF), transforming growth factor (TGF)-α, heparin-binding epidermal growth factor (HB-EGF), orheregulin (HRG)-α. The viability is determined using the MTS assay.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/17627612

    Objective: Antibody-dependent cellular cytotoxicity (ADCC)
    Cells: peripheral blood lymphocytes (PBLs), USPC cell lines (Uterine cancer cell)
    Concentrations: 2.5 μg/mL
    Incubation Time: --
    Method: A standard 5 h chromium (51Cr) release assay was performed to measure the cytotoxic reactivity of peripheral blood lymphocytes (PBLs) from several healthy donors in combination with trastuzumab and/or pertuzumab against USPC target cell lines.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/19920829

    Pertuzumab can apply to nude mice, various cancer cell lines and other related assays (Only for Reference)

动物实验:
- 合并
  • Objective: In vivo tumor growth inhibition studies
    Animal Models: BALB-nu/nu mice
    Formulation: saline
    Dosages: 20 mg/kg, 40 mg/kg and 80 mg/kg
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/21700765

    Objective: To investigate the antitumor activity of the combination of pertuzumab and trastuzumab for gastric cancer
    Animal Models: BALB-nu/nu mice were inoculated subcutaneously with human gastric cancer cell lines MKN-28 or NCI-N87, or an 8-mm3 piece of 4-1ST tumor tissue
    Formulation: saline
    Dosages: 20 mg/kg, 40 mg/kg and 80 mg/kg
    Administration: i.p.
    Reference: https://www.ncbi.nlm.nih.gov/pubmed/21700765

    Ttrastuzumab can apply to immunodeficient mice (eg: SCID mice), various cancer cell lines and other related assays (Only for Reference)

抗体信息

CAS No. 380610-27-5
配制 PBS buffer, pH 7.2
同型 Human IgG1
来源 CHO cells
储存条件 Store the undiluted solution at 4°C in the dark to avoid freeze-thaw cycles

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常见问题及建议解决方法

  • 问题1:

    How to store the antibody?

  • 回答:

    Store the undiluted solution at 4 °C in the dark. Freezing antibodies can result in a loss of activity caused by the freezing/thawing process. Diluting antibodies to working concentrations and storing at 4°C for more than a day should be avoided. Additionally, make sure to keep the antibody sterile. Under these storage conditions, your antibodies should remain active for up to one year and oftentimes longer.

HER2 Signaling Pathway Map

相关HER2产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID