PD0325901(Mirdametinib)

For research use only. Not for use in humans.

Pfizer辉瑞授权 目录号:S1036

PD0325901(Mirdametinib) Chemical Structure

Molecular Weight(MW): 482.19

PD0325901是一种选择性的,非ATP竞争性MEK抑制剂,无细胞试验中IC50为0.33 nM,对ERK1和ERK2磷酸化抑制作用比CI-1040强500倍左右。Phase 2。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1149.21 现货
RMB 580.85 现货
RMB 2226.87 现货
RMB 6305.45 现货
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客户使用Selleck生产的PD0325901(Mirdametinib)发表文献492篇:

产品安全说明书

MEK抑制剂选择性比较

生物活性

产品描述 PD0325901是一种选择性的,非ATP竞争性MEK抑制剂,无细胞试验中IC50为0.33 nM,对ERK1和ERK2磷酸化抑制作用比CI-1040强500倍左右。Phase 2。
靶点
MEK [1]
(Cell-free assay)
0.33 nM
体外研究

PD0325901比另一种MEK抑制剂CI-1040具有更高的渗透率。与CI-1040相比,PD 0325901可以到达体系的更深层。[1]PD0325901是非ATP竞争性的MAPK激酶MEK抑制剂,抑制鼠类结肠26细胞的MEK时IC50为0.33nM。苏氨酸/酪氨酸激酶MEK是RAS/RAF/MEK/ERK信号通路的关键组成部分,在人类肿瘤细胞中通常是激活的。PD0325901精确有效地抑制MEK,作用于MEK1和MEK2时Ki值为1nM。PD0325901作用于细胞ERK1和ERK2的磷酸化效果比CI-1040强到大约500倍。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human CHP-212 cell NYnqTJJvT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MXnJcohq[mm2aX;uJI9nKGi3bXHuJGNJWC1{MUKgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlUzPyCwTT6= M{PK[XNCVkeHUh?=
human M14 cell NFq2eZNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYXaRVlpUW6qaXLpeIlwdiCxZjDoeY1idiCPMUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlQ2KG6PLh?= NXi0ZZB{W0GQR1XS
human SK-MEL-28 cell MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWPhOYxmUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3NSWwuOjhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkWyJI5ONg>? NYS3bm9GW0GQR1XS
human NOMO-1 cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYTJcohq[mm2aX;uJI9nKGi3bXHuJG5QVU9vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuNFchdk1w Mn21V2FPT0WU
human A375 cell NVr6PIxMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NI\lboFKdmirYnn0bY9vKG:oIHj1cYFvKEF|N{WgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2yMlY6KG6PLh?= MWHTRW5ITVJ?
human DU-4475 cell MmnBS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkTFTY5pcWKrdHnvckBw\iCqdX3hckBFXS12NEe1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N{42PyCwTT6= MW\TRW5ITVJ?
human C32 cell MUXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MW\Jcohq[mm2aX;uJI9nKGi3bXHuJGM{OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTNwNkegcm0v NX;pW5NkW0GQR1XS
human BPH-1 cell M1H6PWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWX2bGZtUW6qaXLpeIlwdiCxZjDoeY1idiCEUFitNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVMvQTVibl2u M2OwW3NCVkeHUh?=
human CP50-MEL-B cell MUDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFPyUXhKdmirYnn0bY9vKG:oIHj1cYFvKEOSNUCtUWVNNUJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD12LkW4JI5ONg>? NGf0dlNUSU6JRWK=
human H9 cell NVfsSWNYT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIjRbpdKdmirYnn0bY9vKG:oIHj1cYFvKEh7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NT63O{BvVS5? MWrTRW5ITVJ?
human HTC-C3 cell NIDFbVFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVvJcohq[mm2aX;uJI9nKGi3bXHuJGhVSy2FMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUWuPFkhdk1w NIP1S2dUSU6JRWK=
human BHT-101 cell MlnGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3\lNGlvcGmkaYTpc44hd2ZiaIXtZY4hSkiWLUGwNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVYvPyCwTT6= NH7qfVdUSU6JRWK=
human COLO-741 cell M1;nUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUDLZmhCUW6qaXLpeIlwdiCxZjDoeY1idiCFT1zPMVc1OSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTdwMTDuUU4> NGCwVZdUSU6JRWK=
human OVCAR-5 cell NWSxTIxKT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MV;Jcohq[mm2aX;uJI9nKGi3bXHuJG9XS0GULUWgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME23MlgzKG6PLh?= NYLJO2luW0GQR1XS
human A549 cell MnznS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX;BWVByUW6qaXLpeIlwdiCxZjDoeY1idiCDNUS5JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O{45QSCwTT6= M1nIbnNCVkeHUh?=
human SH-4 cell growth M2fXO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkDQTY5pcWKrdHnvckBw\iCqdX3hckBUUC12IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;OD6yOkBvVS5? NELRRVJUSU6JRWK=
human SK-N-AS cell NHy4bo9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NH;nVoFKdmirYnn0bY9vKG:oIHj1cYFvKFONLV6tRXMh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05NjR2IH7NMi=> NFnCdopUSU6JRWK=
human HT-144 cell NULF[G9yT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVr1cZRJUW6qaXLpeIlwdiCxZjDoeY1idiCKVD2xOFQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF05Njl5IH7NMi=> NUS0bnpTW0GQR1XS
human MEL-HO cell NVLibWV3T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4jVSmlvcGmkaYTpc44hd2ZiaIXtZY4hVUWOLVjPJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9PU41QSCwTT6= MlHrV2FPT0WU
human COLO-679 cell NXLpZ3BLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M1e2[WlvcGmkaYTpc44hd2ZiaIXtZY4hS0:OTz22O|kh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOC5yMTDuUS=> Ml61V2FPT0WU
human HuP-T4 cell M2e1XGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoThTY5pcWKrdHnvckBw\iCqdX3hckBJfVBvVESgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNE46QCCwTT6= M{PCXnNCVkeHUh?=
human H-EMC-SS cell NW\UPI41T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MmTOTY5pcWKrdHnvckBw\iCqdX3hckBJNUWPQz3TV{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjB{IH7NMi=> MUnTRW5ITVJ?
human LB2518-MEL cell NYXVUGhRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVrxUodmUW6qaXLpeIlwdiCxZjDoeY1idiCOQkK1NVguVUWOIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuNVMhdk1w MWXTRW5ITVJ?
human HL-60 cell NYHxWWFtT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NYjlXVFLUW6qaXLpeIlwdiCxZjDoeY1idiCKTD22NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEyNjF3IH7NMi=> MULTRW5ITVJ?
human NCI-H1666 cell NV7lSXZLT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIKzSGFKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3INVY3PiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFzLkG3JI5ONg>? MYLTRW5ITVJ?
human A101D cell MnfXS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVPJcohq[mm2aX;uJI9nKGi3bXHuJGEyODGGIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUGuOFUhdk1w MYHTRW5ITVJ?
human RVH-421 cell NE\CWYlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGXyfpJKdmirYnn0bY9vKG:oIHj1cYFvKFKYSD20NlEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yOi54NDDuUU4> MXTTRW5ITVJ?
human Hs-578-T cell MWLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MnXwTY5pcWKrdHnvckBw\iCqdX3hckBJey13N{itWEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzNjd7IH7NMi=> MV3TRW5ITVJ?
human A375 cells NWC2dGZ4WHKxbHnm[ZJifGmxbjDhd5NigQ>? MYS3NkBp M3jVcmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUO3OUBk\WyuczDlfJBz\XO|aX7nJGJTSUZiVk[wNGUhdXW2YX70JIFnfGW{IEeyJIhzeyCkeTDD[YxtKHSrdHXyMYdtdyCjc4PhfUwhUUN3ME2xN{BvVS5? NVGyVmprOjN2N{SzPFg>
human DOK cell NFL5VGRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NEPyc21KdmirYnn0bY9vKG:oIHj1cYFvKESRSzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGzMlQhdk1w Mlv2V2FPT0WU
human Mewo cell M4Pzemdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MkLzTY5pcWKrdHnvckBw\iCqdX3hckBO\XexIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUSuOFUhdk1w MlexV2FPT0WU
human ONS-76 cell M2\Ybmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoeyTY5pcWKrdHnvckBw\iCqdX3hckBQVlNvN{[gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xOE42OSCwTT6= MXTTRW5ITVJ?
human UACC-257 cell M3T5bGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV3Jcohq[mm2aX;uJI9nKGi3bXHuJHVCS0NvMkW3JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVQvPjJibl2u M4XsU3NCVkeHUh?=
human SW626 cell MmnYS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Ml;ETY5pcWKrdHnvckBw\iCqdX3hckBUXzZ{NjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG0MlkyKG6PLh?= NFuyeWpUSU6JRWK=
human SW620 cell NILzS2lIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXHJcohq[mm2aX;uJI9nKGi3bXHuJHNYPjJyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUSuPVUhdk1w MlTBV2FPT0WU
human TYK-nu cell NEPUfFJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M{\zfWlvcGmkaYTpc44hd2ZiaIXtZY4hXFmNLX71JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVUvOTNibl2u NX;jRnZxW0GQR1XS
human ACN cell NH;PfoxIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M37m[mlvcGmkaYTpc44hd2ZiaIXtZY4hSUOQIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MUWuO|Yhdk1w MmruV2FPT0WU
human MIAPaCa2 cells NE\CbXpRem:uaX\ldoF1cW:wIHHzd4F6 MojiRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPSVHQZWNiOiClZXzsd{whUUN3ME2xO{BvVS5? M4nFUFI{PDd2M{i4
human T-24 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4Hvc2lvcGmkaYTpc44hd2ZiaIXtZY4hXC1{NDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUG5MlcyKG6PLh?= MXnTRW5ITVJ?
human AGS cell MlniS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mn;XTY5pcWKrdHnvckBw\iCqdX3hckBCT1NiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1{MD60NUBvVS5? NVfDbZZzW0GQR1XS
human SW872 cell NUSzRWdzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFfjZmdKdmirYnn0bY9vKG:oIHj1cYFvKFOZOEeyJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NlcvQTlibl2u NF3LOWZUSU6JRWK=
human C2BBe1 cell Mlv5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NX7oUndnUW6qaXLpeIlwdiCxZjDoeY1idiCFMlLC[VEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zQC53NDDuUU4> M335UHNCVkeHUh?=
human MZ7-mel cell MoizS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NVPo[o5pUW6qaXLpeIlwdiCxZjDoeY1idiCPWketcYVtKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OjlwNEOgcm0v M2fueXNCVkeHUh?=
human HCC2998 cell MmThS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGDMNWRKdmirYnn0bY9vKG:oIHj1cYFvKEiFQ{K5PVgh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{Oy54NjDuUU4> NXvldHdkW0GQR1XS
human HO-1-N-1 cell NVP4eIV2T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFXNRXBKdmirYnn0bY9vKG:oIHj1cYFvKEiRLUGtUk0yKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzRwNEOgcm0v NEHyR5ZUSU6JRWK=
human SW756 cell M{DzcWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX\Jcohq[mm2aX;uJI9nKGi3bXHuJHNYPzV4IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;M{SuOFUhdk1w NX3vPG57W0GQR1XS
human NCI-H1437 cell MnKwS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mnr1TY5pcWKrdHnvckBw\iCqdX3hckBPS0lvSEG0N|ch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0{PC52OTDuUU4> Mn[xV2FPT0WU
human NCI-H747 cell MWXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHnod3dKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IO|Q4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OzRwOUigcm0v Mk\UV2FPT0WU
human SK-MEL-2 cell M4\h[2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NX7MOFVUUW6qaXLpeIlwdiCxZjDoeY1idiCVSz3NSWwuOiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTN3LkKgcm0v Mo\1V2FPT0WU
human MZ2-MEL cell NUTZRXV{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkfHTY5pcWKrdHnvckBw\iCqdX3hckBOYjJvTVXMJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9N|UvPjVibl2u NGLJ[ohUSU6JRWK=
human PSN1 cell M3fSVmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MVTJcohq[mm2aX;uJI9nKGi3bXHuJHBUVjFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1|OD60OUBvVQ>? MoDjV2FPT0WU
human CAL-39 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXnJcohq[mm2aX;uJI9nKGi3bXHuJGNCVC1|OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUO5MlA1KG6PLh?= M3uxUnNCVkeHUh?=
human LOXIMVI cell NUL5cJpXT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4f6eWlvcGmkaYTpc44hd2ZiaIXtZY4hVE:[SV3WTUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVM6NjN7IH7NMi=> M{CxOnNCVkeHUh?=
human COLO-792 cell MkPES5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYHJcohq[mm2aX;uJI9nKGi3bXHuJGNQVE9vN{myJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OFMvOTVibl2u M{H3OnNCVkeHUh?=
human CAL-27 cell MmTvS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUHFfnY2UW6qaXLpeIlwdiCxZjDoeY1idiCFQVytNlch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PC57MTDuUS=> MVzTRW5ITVJ?
human AsPC-1 cell MnjGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXv1WnhZUW6qaXLpeIlwdiCxZjDoeY1idiCDc2DDMVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF01PS5{ODDuUU4> NWL1b4hDW0GQR1XS
human NCI-H2291 cell M2C3Omdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4DKemlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNlkyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PDZwNE[gcm0v NEDWN4lUSU6JRWK=
human RCM-1 cell MYjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVzteWxvUW6qaXLpeIlwdiCxZjDoeY1idiCUQ12tNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQ3Njh3IH7NMi=> NXPhWZdwW0GQR1XS
human NCI-H292 cell MVvHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NU\ZeYlPUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFI6OiClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTR5LkO5JI5ONg>? MXfTRW5ITVJ?
human WM-115 cell NVHVOFFkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NEXoUVdKdmirYnn0bY9vKG:oIHj1cYFvKFePLUGxOUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVQ5NjVibl2u Mn\HV2FPT0WU
human RT-112 cell MX3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXfJcohq[mm2aX;uJI9nKGi3bXHuJHJVNTFzMjDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUS4Mlg1KG6PLh?= MkLtV2FPT0WU
human HT-29 cell MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWPJcohq[mm2aX;uJI9nKGi3bXHuJGhVNTJ7IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NUCuOFkhdk1w NEm0fYJUSU6JRWK=
human RKO cell growth NVXkenhqT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MWPJcohq[mm2aX;uJI9nKGi3bXHuJHJMVyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTV{LkCyJI5ONg>? NFi2cmdUSU6JRWK=
human KY821 cell NFvBclBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWPJcohq[mm2aX;uJI9nKGi3bXHuJGt[QDJzIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NUOuN{BvVS5? M4\MdnNCVkeHUh?=
human LB1047-RCC cell NVzGclVNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWG5NYJpUW6qaXLpeIlwdiCxZjDoeY1idiCOQkGwOFcuWkOFIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NUmuOlUhdk1w NUTz[W94W0GQR1XS
human SW1116 cell MnTMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3PM[GlvcGmkaYTpc44hd2ZiaIXtZY4hW1dzMUG2JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OlAvPDlibl2u Mnr1V2FPT0WU
human P12-ICHIKAWA cell M4C5c2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M33YOGlvcGmkaYTpc44hd2ZiaIXtZY4hWDF{LVnDTGlMSVeDIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;NkKuNlchdk1w NGfOO3pUSU6JRWK=
human HCC70 cell M1rkbWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUfhZVhsUW6qaXLpeIlwdiCxZjDoeY1idiCKQ1O3NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVY{NjBzIH7NMi=> MUTTRW5ITVJ?
human MIA-PaCa-2 cell MWDHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHr5N3NKdmirYnn0bY9vKG:oIHj1cYFvKE2LQT3QZWNiNTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD14Mz61N{BvVS5? Mk\SV2FPT0WU
human LoVo cell NGfYT|hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NETwSpdKdmirYnn0bY9vKG:oIHj1cYFvKEyxVn:gZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME22OU4zQSCwTT6= MX\TRW5ITVJ?
human LB2241-RCC cell MkPKS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlfPTY5pcWKrdHnvckBw\iCqdX3hckBNSjJ{NEGtVmNEKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PjVwNUKgcm0v MWnTRW5ITVJ?
human GAK cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M2LLTWlvcGmkaYTpc44hd2ZiaIXtZY4hT0GNIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;Nk[uPFchdk1w M1HITnNCVkeHUh?=
human RD cell Mmi4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUmwWZJ4UW6qaXLpeIlwdiCxZjDoeY1idiCURDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPU[3MlEhdk1w M335ZXNCVkeHUh?=
human KNS-62 cell MYHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3XP[GlvcGmkaYTpc44hd2ZiaIXtZY4hU06VLU[yJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9OlkvQTlibl2u NXS5cFcyW0GQR1XS
human HD-MY-Z cell M{PlXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NYnoXFFOUW6qaXLpeIlwdiCxZjDoeY1idiCKRD3NXU1bKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:PzFwMUKgcm0v MnrwV2FPT0WU
human COR-L105 cell NH;IZmhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWPJcohq[mm2aX;uJI9nKGi3bXHuJGNQWi2OMUC1JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9O|EvPzFibl2u MWXTRW5ITVJ?
human IA-LM cell NV;yVZBRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWnXdIlOUW6qaXLpeIlwdiCxZjDoeY1idiCLQT3MUUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVc{NjJ6IH7NMi=> NYXyS4tFW0GQR1XS
human EM-2 cell NGToZVRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYPJcohq[mm2aX;uJI9nKGi3bXHuJGVONTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD15ND63JI5ONg>? NWnsUpJ[W0GQR1XS
human NB69 cell MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4rlWWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJ4OTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5 M{XSPHNCVkeHUh?=
human HuP-T3 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MV;Jcohq[mm2aX;uJI9nKGi3bXHuJGh2WC2WMzDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUizMlk{KG6PLh?= MUHTRW5ITVJ?
human BB30-HNC cell M1nMOGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGn4TZBKdmirYnn0bY9vKG:oIHj1cYFvKEKEM{CtTG5EKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QDVwNUGgcm0v NInRenFUSU6JRWK=
human HT-1080 cell NX7mTZd4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3zaV2lvcGmkaYTpc44hd2ZiaIXtZY4hUFRvMUC4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVg2Njh6IH7NMi=> M2TN[XNCVkeHUh?=
human RMG-I cell NHrBNlJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M4D6dWlvcGmkaYTpc44hd2ZiaIXtZY4hWk2JLVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME24O{4{PCCwTT6= Mmm4V2FPT0WU
human HCC1419 cell NIXjR2RIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M2TEVWlvcGmkaYTpc44hd2ZiaIXtZY4hUEOFMUSxPUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkyNjN6IH7NMi=> Mn;rV2FPT0WU
human SW780 cell MlP5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NULaT|V7UW6qaXLpeIlwdiCxZjDoeY1idiCVV{e4NEBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVkzNjN{IH7NMi=> NV;GXmwzW0GQR1XS
human SNU-387 cell MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M1;aNmlvcGmkaYTpc44hd2ZiaIXtZY4hW06XLUO4O{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVk{NjN4IH7N NFzzcGJUSU6JRWK=
human LAMA-84 cell NHPCTXBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXP1S5pGUW6qaXLpeIlwdiCxZjDoeY1idiCOQV3BMVg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QTRwNkigcm0v NHn2WZpUSU6JRWK=
human MV-4-11 cell NV6yUlVkT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVjE[4RFUW6qaXLpeIlwdiCxZjDoeY1idiCPVj20MVEyKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:QTRwN{Ogcm0v MkPwV2FPT0WU
human EGI-1 cell NX\Fd|lNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M4ezZ2lvcGmkaYTpc44hd2ZiaIXtZY4hTUeLLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME25OU45OSCwTT6= NXjCO|FWW0GQR1XS
human NCI-SNU-1 cell MkT6S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGPDdoVKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3TUnUuOSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTl4LkezJI5ONg>? NX\GfFJmW0GQR1XS
human MEG-01 cell NEjD[3pIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NUTaWnhQUW6qaXLpeIlwdiCxZjDoeY1idiCPRVetNFEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF06Py55NzDuUU4> NEmweGNUSU6JRWK=
human OMC-1 cell NVnqZ3p4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M3fuVmlvcGmkaYTpc44hd2ZiaIXtZY4hV02FLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNFAvOjNibl2u Ml;2V2FPT0WU
human NB10 cell M4K0Smdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYHJcohq[mm2aX;uJI9nKGi3bXHuJG5DOTBiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMECuOFIhdk1w NGDuboZUSU6JRWK=
human CAL-62 cell M173TWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWjU[4tyUW6qaXLpeIlwdiCxZjDoeY1idiCFQVytOlIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yODBwN{igcm0v M3XWV3NCVkeHUh?=
human NCI-H2087 cell M{DkbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1yxSmlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLViyNFg4KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OTBzLkG0JI5ONg>? M4rLUXNCVkeHUh?=
human MDA-MB-175-VII cell MX7Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M4PhOWlvcGmkaYTpc44hd2ZiaIXtZY4hVUSDLV3CMVE4PS2YSVmgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfS=> NUG1SWVSW0GQR1XS
human LS-513 cell Ml:1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M1fkRmlvcGmkaYTpc44hd2ZiaIXtZY4hVFNvNUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NVE1Njh|IH7NMi=> NFLWd3JUSU6JRWK=
human HN cell growth NITj[2NIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1;uNGlvcGmkaYTpc44hd2ZiaIXtZY4hUE5iY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zMkKuOVkhdk1w Mk\pV2FPT0WU
human ABC-1 cell NGfTNoFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1H2UmlvcGmkaYTpc44hd2ZiaIXtZY4hSUKFLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xNlMvODJibl2u M3XWZnNCVkeHUh?=
human SJSA-1 cell MV3Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NGjrXmhKdmirYnn0bY9vKG:oIHj1cYFvKFOMU1GtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEzOy5zOTDuUU4> M3zZcXNCVkeHUh?=
human PANC1 cells M1fOUGZ2dmO2aX;uJIF{e2G7 NWHCWVdJOTBizszN NUnOfFRpOSCq NUnUc3ZWUW6qaXLpeIlwdiCxZjDNSWsyKGmwIHj1cYFvKFCDTlOxJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCyRYLrNU8zKGyndnXsJIF1KDFyIIXNJIFnfGW{IEGgbJIh[nliV3XzeIVzdiCkbH;0eIlv\yCjbnHsfZNqew>? NW\TTohPOjV5Nk[2N|M>
human MCF7 cells M2XvcWZ2dmO2aX;uJIF{e2G7 NVvRRnB{PzVibXnudy=> NVPHeHRZUW6qaXLpeIlwdiCxZjDNSYsyNzJiaX6gbJVu[W5iTVPGO{Bk\WyuczDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCHUlugdIhwe3Cqb4L5cIF1cW:wIHHmeIVzKDd3IH3pcpMh[nliV3XzeIVzdiCkbH;0eIlv\yCjbnHsfZNqew>? M4DLTFI{Ozl6NEWz

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-ERK / p-AKT / Cyclin D1 / p27; 

PubMed: 22573716     


parental or MEK inhibitor-resistant clonal populations (1182, 1183, and 1187) were treated with increasing doses of PD0325901 for 24 hours and subjected to immunoblot analysis using the indicated antibodies.

p-p70S6K(T389) / p4EBP1(S65) / pS6(S235); 

PubMed: 27699948     


Sensitive (MKN-45, OKAJIMA, HSC-57, GUS, NUGC-4, OCUM-1, SNU-719, and IM95) and resistant (SH-10-TC, MKN-1, HSC-39, NCI-N87, H-111-TC, KE-97, SNU-484, and TMK-1) cell lines were treated with DMSO or PD0325901 (0.1 or 1 μM) for 24 h and then subjected to Western blotting using antibodies against pERK, pAKT, p-p70S6K, p4EBP1, pS6, and GAPDH.

cleaved PARP; 

PubMed: 22573716     


NF1-deficient U251 or LN229 GBM cells were treated with PD0325901 for 4 days and subjected to immunoblot analysis using the indicated antibodies.

DR5; 

PubMed: 25867065     


The indicated cancer cell lines were exposed to the given concentrations of the inhibitors as indicated for 16 h.

22573716 27699948 25867065
Immunofluorescence
Fibronectin / Actin / pFAK; 

PubMed: 28187762     


Representative immunofluorescence images of FNMA by GBM-3 cells treated either with DMSO (b) or PD0325901 (c). Fibronectin is depicted in green and DAPI (blue) was used as counterstain. PD0325901 did not appear to induce FNMA by GBM-3 cells. Rhodamine-phalloidin staining of actin in DMSO-treated (d) or PD0325901-treated GBM-3 cells (e). Note significant cell shape change and actin fiber organization. Scale bar in (e) is 5 μm. Triple stain for actin (red), p-FAK (green) and DAPI (blue) in DMSO-treated (f) and PD0325901-treated (g) GBM-3 cells. PD0325901 appears to induce the localization of p-FAK at sites of cell-ECM attachment. Thirty-micron thick z-stack of DMSO (h) and PD0325901-treated (i) collected by confocal microscopy of multicellular aggregates of GBM-3. Note marked change in actin organization from cortical to stress fibers. Scale bar in (i) is 30 μm.

ZO-1; 

PubMed: 25394671     


16HBE cells were fixed and stained for ZO-1 and DNA. Scale bar, 20 μm.

28187762 25394671
体内研究 PD0325901阻止细胞黑色素瘤细胞系的生长,使异种移植鼠模型的细胞周期停止在G1期,引起细胞凋亡单独口服25 mg/kg PD032590124后,ERK的磷酸化作用被阻断达到50%以上。PD 0325901的抗癌活性已经被用于更广泛的人类移植瘤的研究中,在研究的人类肿瘤模型中,有6/7被PD 0325901明显抑制。[2] PD0325901抑制恶性黑色素瘤细胞系生长。PD0325901抑制TPC-1细胞和K2细胞生长,GI50分别为11和6.3 nM。PD0325901浓度非常低时(10 nM)也明显抑制携带BRAF突变的PTC细胞生长,且同样浓度时只稍微提高携带RET/PTC1重排的PTC细胞生长。PD0325901作用于多种PTC细胞系,有效抑制ERK1/2磷酸化。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

体外级联实验:

在有含p44MAPK(GST-MAPK)的谷胱甘肽S-转移酶融合蛋白和含p45MEK(GST-MEK)的谷胱甘肽S-转移酶融合蛋白存在情况下,测定32P渗透到髓鞘碱性蛋白(MBP)的渗透率。实验溶液包括20 mM HEPES,pH 为7.4,10 mM MgCl2,1 mM MnCl2,1 mM EGTA,50 mM [gamma-32P]ATP,10 mg GST-MEK,0.5 mg GST-MAPK,和40 mg MBP,终体积为100 mL。实验进行20分钟后,加入三氯乙酸终止反应,然后通过GF/C过滤器过滤。使用1205 Beta板测定保留在过滤器中的32P。测定不同剂量PD0325901,绘制剂量反应曲线。
细胞实验:

[3]

- 合并
  • Cell lines: PTC细胞
  • Concentrations: 0.1 nM- 1 μM
  • Incubation Time: 48小时
  • Method:

    1×104个PTC细胞接种在含1mL培养基的24孔板上,在37oC下温育4天。在实验第一天加入不同浓度MEK抑制剂,重复三次。MTT溶于0.8% NaCl溶液,浓度为5 mg/mL,实验第三天,每孔加入0.2 mL,测定GI50或者测定每天的细胞生长曲线。细胞和MTT在37oC下温育3小时。然后从孔中吸出液体除去。染色的细胞溶于0.5 mL DMSO,然后使用Synergy HT增殖酶标仪在570 nm处测定吸光值。然后测定GI50, 按公式 100×(T − T0)/(C − T0)计算细胞生长。


    (Only for Reference)
动物实验:

[3]

- 合并
  • Animal Models: 携带PTC细胞的Ncr-nu/nu鼠
  • Formulation: 80 mM柠檬酸缓冲液(pH 7)
  • Dosages: 20-25 mg/kg
  • Administration: 口服饲喂
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 96 mg/mL (199.09 mM)
Ethanol 40 mg/mL (82.95 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 482.19
化学式

C16H14F3IN2O4

CAS号 391210-10-9
储存条件 粉状
溶于溶剂
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    Whether the inhibitor PD0325901 interacts with other targets other than MEK?

  • 回答:

    PD0325901 has very high selectivity to MEK. In addition, it can also inhibits VEGF activity according to the reference: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2713590/

MEK Signaling Pathway Map

MEK Inhibitors with Unique Features

相关MEK产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID