Bleomycin (NSC125066) sulfate

For research use only. Not for use in humans.

目录号:S1214 中文名称:硫酸博莱霉素

Bleomycin (NSC125066) sulfate Chemical Structure

CAS No. 9041-93-4

Bleomycin Sulfate (NSC125066)硫酸博来霉素是一种糖肽类抗生素,也是一种鳞状细胞癌(SCC)抗癌剂,在UT-SCC-19A细胞中IC50为4 nM。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 3415.58 现货
RMB 1630.13 现货

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客户使用Selleck生产的Bleomycin (NSC125066) sulfate发表文献55篇:

质量管理及产品安全说明书

Antineoplastic and Immunosuppressive Antibiotics抑制剂选择性比较

生物活性

产品描述 Bleomycin Sulfate (NSC125066)硫酸博来霉素是一种糖肽类抗生素,也是一种鳞状细胞癌(SCC)抗癌剂,在UT-SCC-19A细胞中IC50为4 nM。
体外研究

UT-SCC-12A和 UT-SCC-12B均对Bleomycin sulfate耐药,IC50分别为14.2 nM 和13 nM。[1]肺泡巨噬细胞与0.01 μg/mL到1μg /mL Bleomycin sulfate培养18小时显著比巨噬细胞单独培养分泌更多的成纤维细胞生长因子。Bleomycin sulfate刺激的巨噬细胞持续产生大量成纤维细胞生长因子,即使在移去Bleomycin sulfate,用新鲜培养基(不含Bleomycin sulfate)取代之后,该作用依然存在。Bleomycin sulfate刺激肺泡巨噬细胞导致的纤维素生长因子分泌被环己酰亚胺,和5-脂氧合酶抑制剂NDGA(去甲二氢愈创木酸)和BW755c抑制,表明其不仅需要蛋白质合成,也需要花生四烯酸代谢的5-脂氧合酶途径活性完全表达。[2] Bleomycin sulfate (400 µg/mL)培养24小时,降低NTera-2细胞的活性,增加caspase-3,-8和-9的活性,增加Bax和细胞质细胞色素C水平,并降低Bcl-2水平。[3]对于不稳定性畸变,Bleomycin sulfate对ADIPO-P2细胞的致染色体断裂作用在处理后至少持续10天。此外,暴露于Bleomycin sulfate的细胞处理10天后,端粒融合体的外观表明Bleomycin sulfate能够诱导延迟的端粒失稳。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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POLK WT M1XscGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHzb2pOUUN3ME2xNU446oDLwsJihKkyNjgEoN88US=> MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB|MUSwNEc,OjZyM{G0NFA9N2F-
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POLK CD NYfHc4QzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTVwNUpihKnDueLCiUGuNFDDqM7:TR?= NFWzTVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkCzNVQxOCd-Mk[wN|E1ODB:L3G+
RLE/Abca3 MUDGeY5kfGmxbjDBd5NigQ>? MVuwMlTDqM7:TR?= NUPr[3JWOTR2wrDo MmHObY5lfWOnczDhJI1wenCqb3zv[4lk[WxiY3jhcodmKG[{b32g[ZBqfGinbHnhcE1tcWunIH3vdpBpd2yxZ4mgeI8h[SC5aXTlMEB{eHKnYXStc5V1KG2xcoDoc4xw\3oEoB?= M{HFOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3N{[wOVM5Lz5{NUe2NFU{QDxxYU6=
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PMCs MoDxSpVv[3Srb36gRZN{[Xl? MXqwMlLDqM7:Zz;tcC=> MlzqNlTjiJN5MtMgbC=> NFTpbZlqdmS3Y3XzJIlv[3KnYYPld{BqdiC4aX3lcpRqdiCjbnSg{tEuW02DIIDyc5RmcW5? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV7NU[0Nkc,OjV3OUW2OFI9N2F-
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NCM460 NEfFWmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlHRNE0yODBizsznM41t Mnf6OFghcA>? Mn23bY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVzOEKwNUc,OjN3MUiyNFE9N2F-
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NTera-2 NG\RbZdHfW6ldHnvckBCe3OjeR?= MmX5NVIxKM7:Zz;tcC=> MmfxO|IhcA>? NIDIXVV{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDURmFTWyCuZY\lcJM> NILF[Jk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyOVM2PSd-MkK4NlU{PTV:L3G+
NCCIT MnfXSpVv[3Srb36gRZN{[Xl? NUDYcYc{OTJyIN88[{9udA>? NV;LV41nPzJiaB?= MWHzbYdvcW[rY3HueIx6KGmwY4LlZZNmeyCWQlHSV{Bt\X[nbIO= NUfYdY5[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlU{PTVpPkKyPFI2OzV3PD;hQi=>
NCCIT M2m5[GZ2dmO2aX;uJGF{e2G7 M{fCXVEzOCEQvHevcYw> M{Pqb|czKGh? NV\IfnZ[e2mpbnnmbYNidnSueTDk[YNz\WG|ZYOgS3NJKGyndnXsdy=> NI\xV2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyOVM2PSd-MkK4NlU{PTV:L3G+
NTera-2 MnfWSpVv[3Srb36gRZN{[Xl? MUexNlAh|rypL33s MmPWO|IhcA>? NX71RnIxe2mpbnnmbYNidnSueTDpcoNz\WG|ZYOgVJJwfGWrbjDjZZJjd267bDDs[ZZmdHN? M3fES|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK1N|U2Lz5{MkiyOVM2PTxxYU6=
NCCIT NUjDTpV4TnWwY4Tpc44hSXO|YYm= NYO2WHZEOTJyIN88[{9udA>? NXPXNXN4PzJiaB?= NFnYc5h{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDQdo91\WmwIHPhdoJwdnmuIHzleoVtew>? MlnEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MkWzOVUoRjJ{OEK1N|U2RC:jPh?=
NTera-2 NI\qd21HfW6ldHnvckBCe3OjeR?= MoTkNVIxKM7:Zz;tcC=> NIDB[lg4OiCq NE\weFN{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDMVG8hdGW4ZXzz M2DUcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK1N|U2Lz5{MkiyOVM2PTxxYU6=
NCCIT NEXBUlJHfW6ldHnvckBCe3OjeR?= Mn7sNVIxKM7:Zz;tcC=> M173VlczKGh? M2HrbJNq\26rZnnjZY51dHliaX7jdoVie2W|IFzQU{Bt\X[nbIO= MkjjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6MkWzOVUoRjJ{OEK1N|U2RC:jPh?=
MDA-MB-468 M1joc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4e0e|DjiJN{MEFCpO69\y:vTB?= NU\QZ4xZOjRiaB?= MnnSbY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> NVGyNYxlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NVkyQTZpPkKyPFE6OTl4PD;hQi=>
231-H2N NUe4XpZxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVSw5qCUOjBywrFOwIcwdUx? NXz3XXBLOjRiaB?= MkHUbY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjhzOUG5Okc,OjJ6MUmxPVY9N2F-
A549  MXrGeY5kfGmxbjDBd5NigQ>? MVOyNFAwPDByIN88US=> Mlm0doV{fWy2czDpckBiKGSxc3WtdoV{eG:wc3n2[UBqdmO{ZXHz[UBqdiCUT2O= Ml7EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5N{O2PVcoRjJ{N{ezOlk4RC:jPh?=
A549  NH3GRm9HfW6ldHnvckBCe3OjeR?= NVXpUZpUOC12MECg{txO MVeyMVQ5KGh? M4XOdoNifXOnczDtc5JmKG22RF7BJJRp[W5iblTORUBl[W2jZ3W= MljXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5N{O2PVcoRjJ{N{ezOlk4RC:jPh?=
A549  NGHxeWhHfW6ldHnvckBCe3OjeR?= M4fZXVExOCEQvF5CpC=> NEjEeIUx6oDVNEigbC=> NFK5eHBk[XW|ZYOgZ4F{eGG|ZT2zJIFkfGm4YYTpc44> MoXYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ5N{O2PVcoRjJ{N{ezOlk4RC:jPh?=
A549  MVTGeY5kfGmxbjDBd5NigQ>? MYSxNFAh|ryPwrC= M3\NW|DjiJN2ODDo MmPwZ4F2e2W|IGDLSFEh[2ynYY\h[4U> NH:2S5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke3N|Y6Pyd-MkK3O|M3QTd:L3G+
A549  M3uxPWZ2dmO2aX;uJGF{e2G7 MlTGNVAxKM7:TdMg MVuw5qCUPDhiaB?= NIrOOYtk[XW|ZYOgcYl1d2Oqb37kdolidCCub3PhcIl7[XSrb36gc4YhSmG6 NIjhW5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke3N|Y6Pyd-MkK3O|M3QTd:L3G+
A549  MWDGeY5kfGmxbjDBd5NigQ>? MUexNFAh|ryPwrC= MXKw5qCUPDhiaB?= MlXy[IVkemWjc3XzJG1OWCCjbnSgRXRRKGyndnXsdy=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd5M{[5O{c,OjJ5N{O2PVc9N2F-
MCF-7/Her-18  NXP2Vmp6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWPxSJJ7OC52Mj2xO|AxKM7:Zz;tUC=> NEmzXYQzPCCq NG\pRmpqdmirYnn0d{Bk\WyuIHfyc5d1cCCmb4PlJIRmeGWwZHXueIx6 MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjZ{MUSwOEc,OjJ4MkG0NFQ9N2F-
MCF-7 MlH5VGRVN1CFSTDUdoVifG2nboS= Mnn6NE4zPSEQvHevcYwv NF3LflgyNTRiaB?= MY\zbI94eyC|aXfubYZq[2GwdDCoVOKhRCByLkC1LUBkgXSxdH;4bYMh\W[oZXP0 NYjDclVSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1PVEzQDRpPkKyOVkyOjh2PD;hQi=>
MDA-MB-231 MYfQSHQwWEOLIGTy[YF1dWWwdB?= NFP4Zo0xNjJ3IN88[{9udC5? MkizNU01KGh? Ml62d4hwf3Nic3nncolncWOjboSgLHDDqDxiMD6wOUkh[3m2b4TvfIlkKGWoZnXjeC=> NHLvd2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW5NVI5PCd-MkK1PVEzQDR:L3G+
MDA-MB-235  NILFPZlRTFRxUFPJJHRz\WG2bXXueC=> MWKwMlI2KM7:Zz;tcE4> NYHqSJQ{OS12IHi= NE\NZZh{cG:5czDzbYdvcW[rY3HueEApWMLiPDCwMlA2MSCleYTveI95cWNiZX\m[YN1 MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV7MUK4OEc,OjJ3OUGyPFQ9N2F-
MCF-7 NX\INlVpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2C0U|AuOiEQvHevcYwv MkH0NlQwPDhiaB?= NF3lZVdGSzVyPUGuNkDPxGdxbVy= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV7MUK4OEc,OjJ3OUGyPFQ9N2F-
NCCIT  MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXZTnEzOOLCk{G0NOKh|rypL33s MkfHO|IhcA>? MWLMSFUxRTF{MNMg{txoN22uwrC= M{XJ[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NU[yNVYxLz5{MkW2NlE3ODxxYU6=
NCCIT  NIXXSHJHfW6ldHnvckBCe3OjeR?= MlvsO|IhcA>? Mle2d4lodmmoaXPhcpRtgSCrbnPy[YF{\XNiY3HzdIF{\S1|IHHjeIl3cXS7wrC= NV7Ee5p5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OlIyPjBpPkKyOVYzOTZyPD;hQi=>
NCCIT  MXvGeY5kfGmxbjDBd5NigQ>? M4HrOVczKGh? NHXIWJJ{cWewaX\pZ4FvfGy7IHnuZ5Jm[XOnczDjZZNx[XOnLUigZYN1cX[rdIpCpC=> M4LhVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NU[yNVYxLz5{MkW2NlE3ODxxYU6=
NCCIT  MYrGeY5kfGmxbjDBd5NigQ>? NFf1Spg4OiCq MWTzbYdvcW[rY3HueIx6KGmwY4LlZZNmeyClYYPwZZNmNTliYXP0bZZqfHoEoB?= NEfQeYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW2NlE3OCd-MkK1OlIyPjB:L3G+
NCCIT  NEjTW3FHfW6ldHnvckBCe3OjeR?= M17nelczKGh? M{SzUJNq\26rZnnjZY51dHliaX7jdoVie2W|IFLBXEBt\X[nbB?= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV4MkG2NEc,OjJ3NkKxOlA9N2F-
NCCIT  NV;y[oZwTnWwY4Tpc44hSXO|YYm= M4TBXlczKGh? MYHzbYdvcW[rY3HueIx6KGSnY4LlZZNmeyC2aHWgRoNtNTJiY3;ueIVvfA>? NWW4eXZ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK1OlIyPjBpPkKyOVYzOTZyPD;hQi=>
NCCIT  NXWzOIxjTnWwY4Tpc44hSXO|YYm= MXm3NkBp NEjkNW9{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczD0bIUhS3m2LXOgcIV3\Wx? MkjXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3NkKxOlAoRjJ{NU[yNVYxRC:jPh?=
HeLa MULGeY5kfGmxbjDBd5NigQ>? NFPlOm8{OC95MDFOwIcwdWx? MW[yOOKhcA>? NYL5fmJ3cW6lcnXhd4V{KHSqZTDtVm5CKGyndnXsd{Bw\sLicEWz89yNSVSPLICyNeKh[W6mwrDHRWRFPDV? M2e2S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEi3PVM4Lz5{MkS4O|k{PzxxYU6=
MCF-7  MoewSpVv[3Srb36gRZN{[Xl? M4PVXlMxNzdyIN88[{9udA>? M3TsRVI1yqCq MlK1bY5kemWjc3XzJJRp\SCvUl7BJIxmfmWuczDv[uKheDV|78{MRXRONHB{MdMgZY5lyqCJQVTEOFU> Mmn0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2OEe5N|coRjJ{NEi3PVM4RC:jPh?=
HeLa MYrDfZRwfG:6aXPpeJkhSXO|YYm= NYXsVG9qOzBxN{Cg{txoN22u NYrzU|F4OjUEoHi= NFzB[21qdmS3Y3XzJIhq\2iueTDzbYdvcW[rY3HueEBt\X[nbIOgc4Yh\nKjZ33lcpRm\CCGTlGgdoVt\WG|ZTDpcpRwKHSqZTDjfZRweGyjc32= MmXlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2OEe5N|coRjJ{NEi3PVM4RC:jPh?=
MCF-7  MoX2R5l1d3SxeHnjbZR6KEG|c3H5 M1WydVMxNzdyIN88[{9udA>? M4D3OVI1yqCq MmO0bY5lfWOnczDobYdpdHlic3nncolncWOjboSgcIV3\Wy|IH;mJIZz[WevZX70[YQhTE6DIILlcIVie2ViaX70c{B1cGViY4n0c5Bt[XOv NUnpN5JkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0PFc6OzdpPkKyOFg4QTN5PD;hQi=>
NT2 NVfiVmhCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYPtZWFCOTByLU[wNOKh|rypL33s MoSyNlQhcA>? NWT6TmFNUUN3ME20NFAhyrWpL33s NWrhbJVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0Olk6PTJpPkKyOFY6QTV{PD;hQi=>
NT2 NHHFemZHfW6ldHnvckBCe3OjeR?= M2fGbFQxOMLizsznM41t M4PUclI1KGh? M3v3PJNq\26rZnnjZY51dHliaX7jdoVie2W|IHPhd5Bie2VvMzy4MFkh[WO2aY\peJnDqA>? NUnwbnZ4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0Olk6PTJpPkKyOFY6QTV{PD;hQi=>
NT2 MkL0SpVv[3Srb36gRZN{[Xl? MmG0OFAxyqEQvHevcYw> NUHDdGQ{OjRiaB?= MmDtd4lodmmoaXPhcpRtgSCrbnPy[YF{\XNiQlHYJIxmfmWu MkjiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2Nkm5OVIoRjJ{NE[5PVUzRC:jPh?=
NT2 NYDJdZhQTnWwY4Tpc44hSXO|YYm= NGDmd5c1ODEEoN88[{9udA>? M1;4[VI1KGh? NHmxWpZ{cWewaX\pZ4FvfGy7IHTlZ5Jm[XOnczDCZ4wuOiCuZY\lcC=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4OUm1Nkc,OjJ2Nkm5OVI9N2F-
CHO MYXGeY5kfGmxbjDBd5NigQ>? NXPwdoZCOi53wrFOwIcwdWx? NYjnW4h3OThiaD:2JIQ> M2fWTolv\HWlZYRCpJBmenOrc4TlcoNmKG:oIHPodo9ud3OxbXWg[IFu[Wen M{LFSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkOwNVk2Lz5{MkKzNFE6PTxxYU6=
Jurkat MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUSwMVExOMLizsznM41t MkDCNlQhcA>? NE\lVWxienKnc4TzJJRp\SClZXzsJIN6[2ynIHH0JJRp\SCJMjDwbIF{\Q>? NV7i[WJJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNlM{OzJpPkKyNlI{OzN{PD;hQi=>
Jurkat MWTGeY5kfGmxbjDBd5NigQ>? NX7nS2M3OzEEoN88[{9udA>? M2fWZlI1KGh? MXPpcoNz\WG|ZYOgeIhmKHC{b4TlbY4hdGW4ZXzzJI9nKGO7Y3zpcpMhTDNuIFGgZY5lKEJz MmDhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MkOzN|IoRjJ{MkKzN|MzRC:jPh?=
Jurkat MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUiwMVExOMLizsznM41t MUOyOEBp NHfyNopqdmirYnn0d{Bk\WyuIIDyc4xq\mW{YYTpc44hcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZIhf2m2aDDhckBKSzVyII\hcJVmKG:oIEGwNO69\y:vTB?= MlmxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MkOzN|IoRjJ{MkKzN|MzRC:jPh?=
Jurkat MlvUSpVv[3Srb36gRZN{[Xl? Mmr6N|DDqM7:Zz;tcC=> NELobogzPCCq M2XZN4lv[3KnYYPld{B1cGViZX\m[YN1KG:oIID5Z45q\GmxbnWgc44heGixc4Doc5J6dGG2aX;uJIxmfmWuczDv[kBpcXO2b37lJGgzSS6[ MkLjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MkOzN|IoRjJ{MkKzN|MzRC:jPh?=
U2OS EGFPnls MlnuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;BPWlxOC1{wrFOwIcwdWx? NVrzWXN[OjRiaB?= MVHpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 M321bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEGxNFA4Lz5{MUixNVAxPzxxYU6=
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MCF-7 NHnjSG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPtcYNGOC12MECg{txO NF25V3o4OiCq NYfkRVlxVEN3ME2xOVEvQMLizszN NFy1S4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUewN|I6OSd-MkG3NFMzQTF:L3G+
MCF-7/Adr  MkjUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3xNE01ODBizszN MkPUO|IhcA>? MkTsUGM2OD13OD61xsDPxE4EoB?= NXj6c2w6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NFMzQTFpPkKxO|A{OjlzPD;hQi=>
WI-38 NYPQUWIzTnWwY4Tpc44hSXO|YYm= MofnNE81OC96MPMAje69\y:vbNMg M{Dn[|MhcA>? MkXFbY5lfWOnczDhJINwdmOnboTyZZRqd25vZHXw[Y5l\W62IHnuZ5Jm[XOnIHnuJJRp\SCPTjDmdoVyfWWwY4pCpC=> NITwXVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUW5PVYyOid-MkG1PVk3OTJ:L3G+
hBMSC M4DMfWZ2dmO2aX;uJGF{e2G7 NW\LZ2MzOC92MD:4NQKBkc7:Zz;tcOKh M3j5UlMhcA>? M1zjb4lv\HWlZYOgZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDpcoNz\WG|ZTDpckB1cGViTV6g[pJmeXWnbnP5xsA> NWS4VYZNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1PVk3OTJpPkKxOVk6PjF{PD;hQi=>
NCI-H23 M4rJV2Z2dmO2aX;uJGF{e2G7 NWP0eJBDOC92MD:4NQKBkc7:Zz;tcOKh NH35No8{KGh? MWfpcoR2[2W|IHGgZ49v[2WwdILheIlwdi2mZYDlcoRmdnRiaX7jdoVie2ViaX6geIhmKE2QIH\y[ZF2\W6ledMg NXj6N2pVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG1PVk3OTJpPkKxOVk6PjF{PD;hQi=>
A-549 M2nG[mZ2dmO2aX;uJGF{e2G7 MW[wM|QxNzhy4pEJ{txoN22uwrC= MVqzJIg> M{jscIlv\HWlZYOgZUBkd26lZX70doF1cW:wLXTldIVv\GWwdDDpcoNz\WG|ZTDpckB1cGViTV6g[pJmeXWnbnP5xsA> MlPBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3OUm2NVIoRjJzNUm5OlEzRC:jPh?=
PBL  MnzTSpVv[3Srb36gRZN{[Xl? NWi2fll2OC92MD:4NQKBkc7:Zz;tcOKh NIPVNYU{KGh? NH\vUGZqdmS3Y3XzJIEh[2:wY3XueJJifGmxbj3k[ZBmdmSnboSgbY5kemWjc3WgbY4hfGinIF3OJIZz\XG3ZX7jfeKh MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTV7OU[xNkc,OjF3OUm2NVI9N2F-
pol β −/− NYPXTm1YT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFnyPG8xNjJ3LUKwNOKh|rypL33s M4nPblI1yqCq MnrY[IVkemWjc3XzJINmdGxidnnhZoltcXS7IIfpeIghcW6lcnXhd4lv\yClb37j[Y51emG2aX;ud:Kh M{PBXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkWxPVQ1Lz5{MUK1NVk1PDxxYU6=
pol β +/+ M1rvbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIXS[ZUxNjJ3LUKwNOKh|rypL33s Ml[3NlTDqGh? MmL4[IVkemWjc3XzJINmdGxidnnhZoltcXS7IIfpeIghcW6lcnXhd4lv\yClb37j[Y51emG2aX;ud:Kh MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ3MUm0OEc,OjF{NUG5OFQ9N2F-
pol β −/− M4TTW2Z2dmO2aX;uJGF{e2G7 NX\lcIFtOC1{LkWg{txoN22u MYqyJIg> NUnKeWV3[2G3c3XzJGRPSSCmYX3h[4U> NHn5R209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK1NVk1PCd-MkGyOVE6PDR:L3G+
pol β +/+ NIC4ellHfW6ldHnvckBCe3OjeR?= M4SyclAuOi53IN88[{9udA>? Ml3kNkBp M{LqXYNifXOnczDEUmEh\GGvYXfl NE\UO5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK1NVk1PCd-MkGyOVE6PDR:L3G+
pol β −/− NVfVT|V6TnWwY4Tpc44hSXO|YYm= Mn7mNE02KM7:Zz;tcC=> NHrac2g1QCCq MXrjZZV{\XNibXnjdo9vfWOuZYXzJIRwe2ViYX7kJJRqdWViZHXw[Y5l\W62bIm= NWi3W4RNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyOVE6PDRpPkKxNlUyQTR2PD;hQi=>
pol β +/+ M3fBNmZ2dmO2aX;uJGF{e2G7 MmjwNE02KM7:Zz;tcC=> M{LVW|Q5KGh? M3HRUYNifXOnczDtbYNzd263Y3zleZMh\G:|ZTDhcoQhfGmvZTDk[ZBmdmSnboTsfS=> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ3MUm0OEc,OjF{NUG5OFQ9N2F-
TK6  M3fqc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfpelZyOjVvMUWwJO69\y:vbB?= MmPwPVYhcA>? NVu3T4FscW6qaXLpeJMh[2WubDDndo94fGhiZH;z[UBl\XCnbnTlcpRtgQ>? M1vvUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEixOFg4Lz5{MUC4NVQ5PzxxYU6=
TK6  sLUC+Apn1 M{C5W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TLTVI2NTF3MDFOwIcwdWx? NYXjR2RrQTZiaB?= MVvpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6MUS4O{c,OjFyOEG0PFc9N2F-
TK6 sAPE1+Apn1 M1HvVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWHzSIZEOjVvMUWwJO69\y:vbB?= M4j4XVk3KGh? NH;oN2V{cWewaX\pZ4FvfGy7IHnubIljcXS|IHPlcIwh\3Kxd4ToJIRwe2ViZHXw[Y5l\W62bIm= NH7OenY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NVQ5Pyd-MkGwPFE1QDd:L3G+
HCT116 Mn;aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnlPYVTOjVvMUWwJO69\y:vbB?= MmP1PVYhcA>? MlixbY5pcWKrdIOgZ4VtdCCpcn;3eIgh\G:|ZTDk[ZBmdmSnboTsfS=> MknoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEG0PFcoRjJzMEixOFg4RC:jPh?=
HCT116 sLUC+Apn1 NEPOXY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjHOowzPS1zNUCg{txoN22u M2fkOlk3KGh? MYTpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 MojXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEG0PFcoRjJzMEixOFg4RC:jPh?=
HCT116 sAPE1+Apn1 M1;LdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\6NVYzPS1zNUCg{txoN22u NYT0UoVLQTZiaB?= NYW1T4lCe2mpbnnmbYNidnSueTDpcohq[mm2czDj[YxtKGe{b4f0bEBld3OnIHTldIVv\GWwdHz5 M4DrXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEixOFg4Lz5{MUC4NVQ5PzxxYU6=
2SC/20 NITOPZpHfW6ldHnvckBCe3OjeR?= MlPXO|IhcA>? NVixfm17UUN3MDC9JFAvPTF5IN88US=> M1fZdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NEOxNFYxLz5zMkSzNVA3ODxxYU6=
HET-SR-2SC-LNM MmrISpVv[3Srb36gRZN{[Xl? M1iz[|czKGh? M3WyUWlEPTBiPTCwMlUyQSEQvF2= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjR|MUC2NEc,OTJ2M{GwOlA9N2F-
HepG2 NUj6TFBPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nRTGdKPTBiPTCxJO69VQ>? MnXBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNkizOlIoRjF6ME[4N|YzRC:jPh?=
MCF-7 MnT6SpVv[3Srb36gRZN{[Xl? MlzyNlQhcA>? MnfwTWM2OCB;IEWzJO69VQ>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjR5N{O2NUc,OTJ2N{ezOlE9N2F-
COLO-668 NFrwNoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxKD1iMD6wNFAxOjF6IN88US=> M{jRZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
CAL-51 NED3OIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxKD1iMD6wNFAxQTlizszN MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
A427 M1u5dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NE\3d4FKSzVyIE2gNE4xODBzMUig{txO NWnSWFlwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
RMG-I M3r1SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDCTWM2OCB;IECuNFAxOTNzOTFOwG0> MlTpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
HUTU-80 MmPGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYi0fI9KUUN3MDC9JFAvODByMUO1PEDPxE1? MnTaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
NTERA-S-cl-D1 MoHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXuz[4V[UUN3MDC9JFAvODByMUO5NUDPxE1? NWTKemR{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
IST-SL2 M{HZNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXXnSlBuUUN3MDC9JFAvODByMUezNkDPxE1? NGXk[4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
CAL-85-1 NYrVNYVKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGL0epJKSzVyIE2gNE4xODBzN{e3JO69VQ>? M2TMXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
SUP-T1 NEjkfVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGm0NZFKSzVyIE2gNE4xODB{NUGg{txO NFX1coQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
HCC1937 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxKD1iMD6wNFAzPTRizszN Mn;6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
CAL-39 MnPyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPXUlRUUUN3MDC9JFAvODByM{G0O{DPxE1? NVvzfm1KRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
HAL-01 MnfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknkTWM2OCB;IECuNFAxPDV{NzFOwG0> M2Tkd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
OVCAR-8 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXhZW1KSzVyIE2gNE4xODB2N{[zJO69VQ>? NVL2UYw5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
CESS NITnPFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PUUGlEPTBiPTCwMlAxODV2NUeg{txO M4W2R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
NCI-H650 MoS2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjUTWM2OCB;IECuNFAxPTh5MzFOwG0> NXjISGl{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
MKN28 NVjC[2ViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX[wZpd2UUN3MDC9JFAvODBzMEig{txO M2W2NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
RS4-11 MkTZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTJTWM2OCB;IECuNFAyPjR|IN88US=> M3;1N|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
MFH-ino MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX7JR|UxKD1iMD6wNFI3PDZizszN M4XhTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
KYSE-150 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxKD1iMD6wNFI3PDdizszN NHWwTG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw1ODN4NkSvK|5USU6JRWK8M4E,
KGN Ml;aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxKD1iMD6wNFM4OTJizszN MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
HOP-62 MlnDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxKD1iMD6wNFQyOzRizszN NYfmRWh{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
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EW-24 MoTYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFexcFVKSzVyIE2gPVUvOzNzNjFOwG0> NVridYs6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
UACC-812 MlHFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxKD1iOUmuOVk2OSEQvF2= M4qxclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
NCI-H720 MnjnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrMdoZXUUN3MDC9JFExOy5|NUGg{txO MnPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
NCI-H69 M3\DbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxKD1iMUCzMlkxQSEQvF2= NVv4OIE1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
OPM-2 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1zwd2lEPTBiPTCxNFQvODZ4IN88US=> NUjMT|hPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
RD MlrtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnnCTWM2OCB;IEGwOU43QThizszN MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
NCI-H1581 MoTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknSTWM2OCB;IEGxNE4zOjRizszN MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
OVCAR-3 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWTJR|UxKD1iMUKzMlM{OiEQvF2= MlnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
HCC70 M1ny[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnzPTWM2OCB;IEGyOU45PjhizszN MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
GAK MoXsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnJU|R7UUN3MDC9JFEzPi5zM{Kg{txO MoXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOFA{PjZ2Lze+V2FPT0WUPD;hQi=>
K-562 NI\2b|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY\1U3BOUUN3MDC9JFEzPi5{Mkeg{txO NXXpcGNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
UACC-893 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVjJR|UxKD1iMUK5Mlc4PyEQvF2= M3K4blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFQxOzZ4ND:nQnNCVkeHUkyvZV4>
SKG-IIIa NUDEcZZHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{DNZmlEPTBiPTCxN|YvPDZ7IN88US=> NYHj[Ih7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
SHP-77 MlHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1H3bmlEPTBiPTCxOFIvOTN3IN88US=> NVfJWnNlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?
D-263MG M2nofWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mmi4TWM2OCB;IEG0Nk4zODZizszN MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
Mewo NXmzdJBUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfUPVAzUUN3MDC9JFE1Py57NkKg{txO MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly0NFM3PjRxJ{7TRW5ITVJ:L3G+
MSTO-211H MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\ETWM2OCB;IEG0PU41PTlizszN NW\SUYtORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPDB|Nk[0M{c,W0GQR1XSQE9iRg>?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
LRP6 / Wnt5AB / Dvl2 / β-catenin; 

PubMed: 27649046     


CRL-1490 cells were treated with the indicated concentrations of bleomycin for either 12 or 24 h, and then probed for canonical Wnt proteins LRP6, Wnt5AB, Dvl2, and β-catenin. 

phospho-eIF2α / eIF2α; 

PubMed: 23762310     


Western blotting analysis of phosphorylated, total eIF2α and β-actin in CT26 treated 24 h with the indicated drugs. BLM: Bleomycin

cytochrome c; 

PubMed: 20154224     


mitochondrial release of cytochrome c was detected by Western blot in cytosolic extract and analyzed by band densitometry. Data show the means of four independent experiments ± SE. *Statistical significance from control, P < 0.05. Representative Western blots are shown.

Bcl-xl; 

PubMed: 20154224     


Representative Western blots are shown of whole cell extract using anti-Bcl-xL.

Bcl-2; 

PubMed: 20154224     


Representative Western blots are shown of whole cell extract using anti-Bcl-2.

27649046 23762310 20154224
Immunofluorescence
LC3; 

PubMed: 23762310     


CT26 cells were cultured on glass slides and treated with indicated drugs for 24 h. The cells were then submitted to anti-LC3 (green) and DAPI (blue) labeling and observed under an epifluorescence microscope. BLM: Bleomycin

23762310
ELISA
HMGB1; 

PubMed: 23762310     


HMGB1 was titrated with ELISA method in supernatant of CT26 cells treated 24 h with indicated drugs. BLM: Bleomycin

23762310
体内研究 Bleomycin sulfate处理后第7天,NOX-/- 的BALf中CD45+细胞是WT的1.7倍多,其中57%为Mf,第21天,在WT中减少到67%,在NOX-/-中减少到83%。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[4]
- 合并
  • Cell lines: ADIPO-P2 细胞
  • Concentrations: 2.5 μg/mL
  • Incubation Time: 30分钟
  • Method: ADIPO-P2细胞在D-MEM高葡萄糖培养基中生长,用20%胎牛血清,青霉素(100 U/mL) 和链霉素 (100 μg/mL)增补,在37 °C 和5% CO2大气环境中培养。细胞在包含1.5×105 cells/mL的TC25 Corning培养瓶中单层培养。对于每个实验,建立两个培养瓶,一个用于对照组,一个用于处理培养。在对数生长期,ADIPO-P2细胞用2.5 μg/mL Bleomycin sulfate以30分钟脉冲处理。建立平行对照组,不暴露于Bleomycin sulfate。暴露时间和Bleomycin sulfate浓度根据先前实验室中进行的Bleomycin sulfate处理哺乳动物细胞的研究进行选择。Bleomycin sulfate脉冲处理结束时,细胞用Hank's平衡盐溶液清洗两次,并保存在新鲜培养基中直到采集。处理后5个传代培养期间,将细胞持续保持在培养基中。当培养基中融合(培养基中大约4×105 cells/mL)时,进行传代培养。为估计细胞生长,传代培养时,细胞通过胰蛋白酶化收集,每等份大约200 μL,用0.4%台盼蓝着色,测定活细胞数量。随后细胞悬浮在新鲜培养基中,重新分配到包含1×105 cells/mL的新培养瓶中继续生长。将剩余的细胞丢弃或置于另外的培养瓶中进行细胞遗传学分析,这在处理结束后18小时或第10天进行。为分析染色体突变,培养的最后3小时将colchicine (0.1 μg/mL)加入到细胞培养基。染色体的制备根据标准程序进行。采集后,将细胞低渗震动,固定在甲醇:乙酸(3:1)中,涂布在载玻片上,进行PNA-FISH。进行两个独立的实验。
    (Only for Reference)
动物实验:[7]
- 合并
  • Animal Models: CD-1小鼠
  • Dosages: 5 mg/kg, 2 ml/kg
  • Administration: 鞘内注射给药
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 100 mg/mL warmed (66.11 mM)
Water 100 mg/mL (66.11 mM)
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 1512.62
化学式

C55H85N17O25S4

CAS号 9041-93-4
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    what is the ratio between bleomycin A2 and bleomycin B2 in your product?

  • 回答:

    The content of Bleomycin A2 and Bleomycin B2 is 62% and 29% respectively.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID