Fludarabine

For research use only. Not for use in humans.

目录号:S1491 别名: FaraA, Fludarabinum 中文名称:氟达拉滨

Fludarabine Chemical Structure

CAS No. 21679-14-1

Fludarabine (FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1032.75 现货
RMB 810.59 现货
RMB 2525.07 现货
RMB 3836.33 现货
RMB 7289.1 现货
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客户使用Selleck生产的Fludarabine发表文献94篇:

产品安全说明书

STAT抑制剂选择性比较

生物活性

产品描述 Fludarabine (FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。
靶点
STAT1 [4]
(Vascular smooth muscle cells)
体外研究

Fludarabine有效抑制RPMI8226细胞增殖,呈时间浓度依赖性。Fludarabine处理MM.1S和MM.1R细胞48小时的IC50分别为13.48μg/mL和33.79μg/mL。而Fludarabine处理U266细胞48小时的IC 50为222.2μg/mL。Fludarabine处理可增加处于细胞周期G 1期的细胞数量,并伴随着处于细胞周期S期细胞数量的减少,对细胞周期的影响呈时间依赖性。Fludarabine在MM细胞中诱导细胞周期阻滞和细胞凋亡。Fludarabine时间依赖性的诱导caspase,-8,- 9和- 3,- 7的剪切,继而诱导PARP的剪切 。氟达拉滨以时间梯度增加Bax基因表达,而Bak基因表达量不变。RPMI 8226细胞用Fludarabine处理12小时,处理后细胞膜电位降低,61.05%的细胞表达低荧光罗丹明123,而未处理的对照组细胞仅为8.62%[1]。为提高溶解度,Fludarabine可制为一磷酸盐形式(F-ara-AMP, fudarabine),在静脉滴注后即可即时、定量的从母核上去磷酸化。细胞内重磷酸化修饰导致Fludarabine-阿糖胞苷三磷酸((F-ara-ATP)。该代谢产物抑制了DNA复制的几个关键过程,如DNA合成所需酶,比如核糖核酸还原酶,DNA引发酶,DNA聚合酶,具有3 ' - 5 '外切酶活性的脱氧核糖核酸聚合酶δ和ε和DNA连接酶[2]。通过检测ICAM - 1的表达和IL - 8释放,表明Fludarabine还可以诱导前炎症刺激单核细胞的增加[3]。Fludarabine对卵巢癌细胞系生长无影响,而它对黑色素瘤细胞系有明显的剂量依赖性抑制[4]。Fludarabine诱导显著降低STAT - 1磷酸化,但不改变JAK 2活性。磷酸Fludarabine对三种STAT蛋白磷酸化影响不显着。Fludarabine(1.5mg)在处理2天后和7天后显著降低磷酸化STAT - 1和STAT-1总蛋白量[5]。 磷酸化JAK 2在处理后第2天变化不明显,处理后第7天的JAK表达明显受抑制。Fludarabine不影响其他STAT蛋白质,特异性抑制STAT - 1激活以降低血管平滑肌细胞体外增殖。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  NFzFOGRHfW6ldHnvckBCe3OjeR?= MVGyNEDPxE1? M3PBfVI1KGh? NFnWe4JqdmirYnn0d{BmgHC{ZYPzbY9vKG:oIFnEUy=> NITySYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm0NFcyOid-MkW5OFA4OTJ:L3G+
MV-4-11 NFS0U2tCeG:ydH;zbZMhSXO|YYm= MV:yMlUh|ryP MmDnOFghcA>? M4O0XYlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 NHPJOpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGxNVU5Oyd-MkWxNVE2QDN:L3G+
THP-1 MoLQRZBweHSxc3nzJGF{e2G7 MXuyMlUh|ryP MX60PEBp NVvs[GdicW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> M{HrdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUGxOVg{Lz5{NUGxNVU5OzxxYU6=
MOLM 13 NYKyOGtmSXCxcITvd4l{KEG|c3H5 NVPlN|JrOi53IN88US=> NEjIUWU1QCCq MnnUbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFzMUW4N{c,OjVzMUG1PFM9N2F-
KBM3/Bu2506 NFrQVoFCeG:ydH;zbZMhSXO|YYm= MojENk42KM7:TR?= MUC0PEBp MkLkbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= M{DkXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUGxOVg{Lz5{NUGxNVU5OzxxYU6=
Nalm-6 NXzKOnlFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWW1e5hGUUN3ME2xPEDPxE1? NEjlXlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
Reh MmnvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljDTWM2OD1|MDFOwG0> NX\DPGc6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
U2937 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTKTWM2OD1zNjFOwG0> MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
Mec-1 MlvjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYX3d5FrUUN3MP-8olUxOCEQvF2= NUX0dpdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
RPMI-8226 NWXYT3dlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\BW2lEPTB;NUCwJO69VQ>? MmX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Molt-4 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3zrc2lEPTB;MUiwJO69VQ>? M123PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
Nalm-6-FluR M3iyVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFfLfY9KSzVyPUK1NEDPxE1? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
Raji  M2DpTGZ2dmO2aX;uJGF{e2G7 NIPVNYc{yqEQvF2= MmPkNlQwPDhxN{KgbC=> MVfpcoR2[2W|IHHjZ5VufWyjdHnvcpMhd2ZicEWzMEBxPjNiYX7kJJA4O8Li MnXqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NEC2PVUoRjJ2OUSwOlk2RC:jPh?=
PBMC NEnGd2hHfW6ldHnvckBCe3OjeR?= MYK1NE8yODBizszN NX3kcpdjOjRiaB?= NXLvUXo{TE2VTx?= M1TTO4lvcGmkaYTzJHNVSVRzIIDoc5NxcG:{eXzheIlwdg>? NFLqO|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
MDA-231 M3OzSWZ2dmO2aX;uJGF{e2G7 MmnENVAxKM7:TR?= NVLPT2M3OjRiaB?= NXjxRmlyTE2VTx?= NUWwU3dR\GWlcnXhd4V{KEmGTzDlfJBz\XO|aX;u MlPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
624.38mel  Ml63SpVv[3Srb36gRZN{[Xl? NEXmPWI2OCEQvF2= MX[yOEBp MXrEUXNQ M4fVeIRm[3KnYYPld{BKTE9iZYjwdoV{e2mxbh?= MmW4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
MDA-231 NHi5dI5HfW6ldHnvckBCe3OjeR?= NGLHUFY2OC1{MECg{txO NXPCe4RrOjRiaB?= NFXEUZRFVVOR NVzsemt4cW6qaXLpeJMhUUSRIHHjeIl3cXS7IHnu[IVx\W6mZX70cJkhd2ZibWLORUBt\X[nbIO= NW\ISJRjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
624.38mel  NIXYTGdHfW6ldHnvckBCe3OjeR?= NHPxXWE2OC1{MECg{txO NFnwV4czPCCq M3PlZWROW09? NYr4SYVpcW6qaXLpeJMhUUSRIHHjeIl3cXS7IHnu[IVx\W6mZX70cJkhd2ZibWLORUBt\X[nbIO= NUCzW2t2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
HMECs M36yfWZ2dmO2aX;uJGF{e2G7 NVnhdIwyOTBywrFOwG3DqA>? NIi5WXM{PsLiaB?= M3rlcIlvcGmkaYTzJGlHVs7|wrDhcoQhVFCVIHnu[JVk\WRiU2TBWFEheGixc4Doc5J6dGG2aX;uJIFv\CCLUl[xJIV5eHKnc4Ppc44> M4jSdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkGxN|I4Lz5{NEKxNVMzPzxxYU6=
HMECs  NEPSPW9HfW6ldHnvckBCe3OjeR?= NEftVXgyODEEoN88UeKh M1nB[VM3yqCq NG\oeIZqdmirYnn0d{BKTk8QsdMgcYVlcWG2ZXSgdIhwe3Cqb4L5cIF1cW:wIH;mJHNVSVRzIHHu[EBUXEGWMzygZpV1KG6xdDDv[kBUXEGWMh?= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzMUOyO{c,OjR{MUGzNlc9N2F-
BJAB MlXCRZBweHSxc3nzJGF{e2G7 M37jd|XDqM7:TR?= NELUU3EzPCCq NGPmdmlqdmS3Y3XzJINmdGxiYYDvdJRwe2m| NV;pR|c2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOVcyPDdpPkK0NFU4OTR5PD;hQi=>
I-83 M1vzSWFxd3C2b4Ppd{BCe3OjeR?= NF7OR2Y2yqEQvF2= MlrDNlQhcA>? NFTrNm1qdmS3Y3XzJINmdGxiYYDvdJRwe2m| MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB3N{G0O{c,OjRyNUexOFc9N2F-
NALM6 NI\YWo9CeG:ydH;zbZMhSXO|YYm= M1\o[lXDqM7:TR?= NGridHAzPCCq NFPTO3JqdmS3Y3XzJINmdGxiYYDvdJRwe2m| Mn30QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNUexOFcoRjJ2MEW3NVQ4RC:jPh?=
DU-145 MmPES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\IV|IxNTFyIN88[{9udA>? NIrHZpU1QCCqwrC= MWPpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NUPVXlNDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3N|Q5OTVpPkKzO|M1QDF3PD;hQi=>
Nalm-6 MUTGeY5kfGmxbjDBd5NigQ>? NIXTN28yOMLizszN NH\JPWIyNzJxNDDo MWPpcoR2[2W|IHH1eI9xcGGpeR?= M2fMV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkixNlI{Lz5{M{[4NVIzOzxxYU6=
Reh MUjGeY5kfGmxbjDBd5NigQ>? MYCxNOKh|ryP M3\rSlEwOi92IHi= NF7IOYhqdmS3Y3XzJIF2fG:yaHHnfS=> Mk\pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEGyNlMoRjJ|NkixNlI{RC:jPh?=
Nalm-6 NYrFbHhbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{LQcGlEPTBi4pk8NVDjiIoQvF2= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MUKyN{c,OjN4OEGyNlM9N2F-
Reh NVm3d4c6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoryTWM2OCEkiMyxNQKBkc7:TR?= NETjTYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NVIzOyd-MkO2PFEzOjN:L3G+
HEC1A NIC1ZnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rPeVExOC13MECg{txO MYWyOEBp MXnpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
AN3CA NEnF[IRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUixNFAuPTByIN88US=> MmTMNlQhcA>? MnPDbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> M3XU[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
HEC50B M{PDeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoPpNVAxNTVyMDFOwG0> MYSyOEBp NHnxRVJqdmirYnn0d{Bk\WyuIHfyc5d1cCC|bHnnbJRtgQ>? MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
HEC1A M{PyVWFxd3C2b4Ppd{BCe3OjeR?= MVOyNE8yODBizszN NUWxRYk6OjRiaB?= M2jNdIlv\HWlZYOgZZBweHSxc3nzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
AN3CA NX23bFZ[SXCxcITvd4l{KEG|c3H5 Ml7VNlAwOTByIN88US=> M1PneVI1KGh? MV7pcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NFLvTnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
HEC50B M1fTfWFxd3C2b4Ppd{BCe3OjeR?= NFPZWGszOC9zMECg{txO NXThXGd{OjRiaB?= NVLYXmVxcW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> NYe1flFwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
EHEB M{D0eWFxd3C2b4Ppd{BCe3OjeR?= Mln6OFAh|ryP M3H4clI1KGh? NYLJOWRtcW6mdXPld{BieG:ydH;zbZM> M372TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEm3NFc2Lz5{M{S5O|A4PTxxYU6=
A549 NHzOOVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXVdHVlUUN3ME2xOU44yrF{LkigxtVO MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN5N{G5Nkc,OjN|N{exPVI9N2F-
A549 GAPDH-deficient MnT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3SwV2lEPTB;MUiuOeKyOi5|INM1US=> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN5N{G5Nkc,OjN|N{exPVI9N2F-
CLL  MVzBdI9xfG:|aYOgRZN{[Xl? Mn7HNVAh|ryPwrC= Moj4NlQuQTZiaB?= NHWx[JRqdmS3Y3XzJIFxd3C2b4TpZ{Bk\WyuIHTlZZRp NX3zWnQ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNFc3QDZpPkKyNlA4Pjh4PD;hQi=>
MEC1 NWXYdYVOSXCxcITvd4l{KEG|c3H5 M4THUFExOMLizszN Mn;pO|IhcA>? NF3RcWdqdmS3Y3XzJIFxd3C2b4Ppd{B{cWewaX\pZ4FvfGy7 M2C0TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MUOyPVc{Lz5{MkGzNlk4OzxxYU6=
U937  NFTRUHZCeG:ydH;zbZMhSXO|YYm= NInSfogxNjhizszN MkewOE01QCCq MnzzbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= Mnm5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{S3NFAoRjJ{MEe0O|AxRC:jPh?=
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Daudi NVLGT2E6SXCxcITvd4l{KEG|c3H5 MlfqNlAh|ryP MWS5OkBp NITPZVRqdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> NHPjbJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCyN|UzOyd-MkKwNlM2OjN:L3G+
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EHEB M1[2Z2Z2dmO2aX;uJGF{e2G7 MmH6OU02OCEQvF2= NX\G[VFIOjRiaB?= MoSy[IVkemWjc3XzJJAzOSCneIDy[ZN{cW:wIIPp[45q\mmlYX70cJk> NHfYcGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUG2PFM6OSd-MkGxOlg{QTF:L3G+
JVM-2  M2LzZmZ2dmO2aX;uJGF{e2G7 NWe3TItsOzBizszN Mk\BNlQhcA>? M{juU4Rm[3KnYYPld{BxOjFiZYjwdoV{e2mxbh?= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF4OEO5NUc,OjFzNkizPVE9N2F-
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MDA-MB-231 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVK1RotuUUN3ME20MlAh|ryP MnXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NEezPVAoRjJyNES3N|kxRC:jPh?=
MCF-7 NHrLe3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVHWb5l4UUN3ME2xOU4xKM7:TR?= NIfHRnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES0O|M6OCd-MkC0OFc{QTB:L3G+
HLE-B3  M{K0SmZ2dmO2aX;uJGF{e2G7 NEX3TmgzPSEQvF2= MoTTOFghcA>? M4nGdIJtd2OtczDJSm4u|rQkgKPpcoR2[2WmIGPURXQyKHCqb4PwbI9zgWyjdHnvckBidmRiSVTPJIV5eHKnc4Ppc44> NEnSSGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MESzOVE2QCd-MkC0N|UyPTh:L3G+
K562 NVvDTXVvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\pTotYPzJiaB?= MUXJR|UxRTNwMzDuUS=> M3TlOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{C3NVk5Lz5{MEOwO|E6QDxxYU6=
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GR-145 NXLuZW1FT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4rHXWlEOjB;Mj63OEDEnyBzMPMIllghKM7:TdMg M4nXW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Nk[xN|gxLz5zOE[2NVM5ODxxYU6=
A549 NXLQNnRCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mk\LTWMzOD13LkS4JOOYKDFy4pkSPEDPxE4EoB?= NV;vSXJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2OlE{QDBpPkG4OlYyOzhyPD;hQi=>
CaSki  MmjwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrEdmVKSzJyPUGuN|chy5diMUFijLI4KM7:TdMg M{\PdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Nk[xN|gxLz5zOE[2NVM5ODxxYU6=
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CLL5 NF\UdYtCdnSrdIXtc5Ih[XO|YYm= MYG0PEBpenN? M3;ROWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JGNNVDViY3XscJMhcXOxbHH0[YQh\nKxbTDDUGwheGG2aXXueEBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCjbnHsfZNqeyxiRVO1NEA:KDBwMU[g{txONg>? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5M{ezPUc,OjR4N{O3N|k9N2F-
K562 NEW1enVEgXSxdH;4bYNqfHliYYPzZZk> NF\JUpo4OiCqcoO= Ml;UR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gdIFkdGm2YYjlcE1z\XOrc4ThcpQhUzV4MjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5{NjFOwG0v M3rZVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNkC1OlU3Lz5{ME[wOVY2PjxxYU6=
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primary CLL NISzUItEgXSxdH;4bYNqfHliYYPzZZk> NFHrUHlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBxemmvYYL5JGNNVCClZXzsd{whVER3MDC9JFEvOSEQvF2u MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF2OEO5Nkc,OjVzNEizPVI9N2F-
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SK-N-MC NHjBN3RyUFSVIHHzd4F6 M3rRdJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz M1vGfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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LAN-5 MUHxTHRUKGG|c3H5 MkXUdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| NWK5eXQ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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NB1643 MXjxTHRUKGG|c3H5 NFf1[nhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBPSjF4NEOgZ4VtdHN? M33uVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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SJ-GBM2 MlS3dWhVWyCjc4PhfS=> M2HXS5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNLNUeETUKgZ4VtdHN? NWTobFRjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 NHqz[VRyUFSVIHHzd4F6 NI\4d3dyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBVSzN{IHPlcIx{ MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh18 NGLzXJlyUFSVIHHzd4F6 M1WwWJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpOThiY3XscJM> MoL6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Saos-2 M4e0RpFJXFNiYYPzZZk> MlzzdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV4Fwey1{IHPlcIx{ Mlv6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 MVvxTHRUKGG|c3H5 M4OweZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KE:{dHjv[49v[WxiM1Sgeoli[mmuaYT5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= Mm\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
RD M{TGN5FJXFNiYYPzZZk> NHjZc2NyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFKGIHPlcIx{ MljXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
procaspase-9 / procaspase-3; 

PubMed: 27223263     


Procaspase-9 and procaspase-3 were analyzed by Western blot in CLL cells from three patients after a 48 h-incubation in the absence or presence of 3 μM aphidicolin (APC) with or without fludarabine at 0.3 and 1 μM. β-Actin served as a loading control.

p-p53 / p53; 

PubMed: 27223263     


(A–B) CLL cells from 3 different patients were incubated for 24 h with fludarabine at the indicated concentrations in the absence or presence of 3 μM aphidicolin (APC). Phosphorylation of p53 at Ser-15 and total p53 were analyzed by Western blot. Data sho䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒

STAT1; 

PubMed: 26677135     


Representative western blot analysis of STAT1 in RAW 264.7 cells treated with STAT1 inhibitor fludarabine (Flu) for 24 h.

27223263 26677135
Immunofluorescence
α-SMA / Vimentin; 

PubMed: 28322315     


FLU (50 μM) reduces the expression of α-SMA and Vimentin protein in primary mouse activated HSCs (Hepatic Stellate Cells).

28322315
Growth inhibition assay
Cell viability ; 

PubMed: 24956101     


CLL cells RPMI/0.1% FBS were cultured on 0.5% BSA or 150 nM MMP-9 for 1 h prior to adding the indicated concentrations of fludarabine (Fluda). After 48 h (Fluda) cell viability was determined by flow cytometry using FITC-Annexin V and PI.

24956101
体内研究 PBS对照组中肿瘤细胞增长迅速,25天内扩增为10倍。而用40mg/kgFludarabine处理的肿瘤扩增不超过5倍。40mg/kgFludarabine对RPMI 8226细胞有明显的抗肿瘤效果。RPMI 8226处理肿瘤细胞十天后增加凋亡细胞核数量。[1] Fludarabine可在免疫缺陷小鼠中有效抑制RPMI 8226细胞的骨髓转移。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: 地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系
  • Concentrations: 2 μg/Ml
  • Incubation Time: 24 小时
  • Method:

    对地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系以5 × 105细胞密度铺板,实验组均包括氟达拉滨处理和空白对照组。处理后细胞用PBS清洗两次然后用70%冰乙醇固定,离心后用含100μg/mL核糖核酸酶PBS重悬浮,37ºC孵育30分钟。样品重悬于25μg/mL碘化吡啶中,用流式细胞仪检测。根据产品说明用Annexin V-FITC试剂盒检测细胞凋亡。利用TUNEL(末端脱氧核苷酸转移酶介导脱氧核苷酸缺口末端标记)法和原位细胞凋亡检测试剂盒处理细胞,最后用流式细胞仪分析。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带RPMI 8226肿瘤细胞的免疫缺陷型小鼠
  • Dosages: 40 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 57 mg/mL (199.83 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
30% propylene glycol, 5% Tween 80, 65% D5W
30 mg/mL (suspension)

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 285.23
化学式

C10H12FN5O4

CAS号 21679-14-1
储存条件 粉状
溶于溶剂
别名 FaraA, Fludarabinum

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04526795 Not yet recruiting Drug: Cytarabine|Drug: Fludarabine|Drug: Pegcrisantaspase Blast Phase Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent Acute Biphenotypic Leukemia|Recurrent Acute Lymphoblastic Leukemia|Recurrent Acute Myeloid Leukemia|Recurrent Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent T-Cell Prolymphocytic Leukemia|Refractory Acute Biphenotypic Leukemia|Refractory Acute Lymphoblastic Leukemia|Refractory Acute Myeloid Leukemia|Refractory Chronic Myelogenous Leukemia BCR-ABL1 Positive|Refractory T-Cell Prolymphocytic Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) December 31 2020 Phase 1
NCT03832127 Not yet recruiting Drug: 18F-Fludarabine Myeloma Nantes University Hospital|Cyceron April 1 2019 Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    how to re-suspend and deliver the inhibitor for in vivo experiments?

  • 回答:

    For S1491, Fludarabine, we tested a vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W that you can resuspend the compound in at up to 30mg/ml. It's a suspension and can only be given via oral gavage.

STAT Signaling Pathway Map

相关STAT产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID