Fludarabine (NSC 118218)

For research use only. Not for use in humans.

目录号:S1491 别名: FaraA, Fludarabinum 中文名称:氟达拉滨

Fludarabine (NSC 118218) Chemical Structure

CAS No. 21679-14-1

Fludarabine (NSC 118218, FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1032.75 现货
RMB 810.59 现货
RMB 2525.07 现货
RMB 3836.33 现货
RMB 7289.1 现货
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客户使用Selleck生产的Fludarabine (NSC 118218)发表文献114篇:

产品安全说明书

STAT抑制剂选择性比较

生物活性

产品描述 Fludarabine (NSC 118218, FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。
靶点
STAT1 [4]
(Vascular smooth muscle cells)
体外研究

Fludarabine有效抑制RPMI8226细胞增殖,呈时间浓度依赖性。Fludarabine处理MM.1S和MM.1R细胞48小时的IC50分别为13.48μg/mL和33.79μg/mL。而Fludarabine处理U266细胞48小时的IC 50为222.2μg/mL。Fludarabine处理可增加处于细胞周期G 1期的细胞数量,并伴随着处于细胞周期S期细胞数量的减少,对细胞周期的影响呈时间依赖性。Fludarabine在MM细胞中诱导细胞周期阻滞和细胞凋亡。Fludarabine时间依赖性的诱导caspase,-8,- 9和- 3,- 7的剪切,继而诱导PARP的剪切 。氟达拉滨以时间梯度增加Bax基因表达,而Bak基因表达量不变。RPMI 8226细胞用Fludarabine处理12小时,处理后细胞膜电位降低,61.05%的细胞表达低荧光罗丹明123,而未处理的对照组细胞仅为8.62%[1]。为提高溶解度,Fludarabine可制为一磷酸盐形式(F-ara-AMP, fudarabine),在静脉滴注后即可即时、定量的从母核上去磷酸化。细胞内重磷酸化修饰导致Fludarabine-阿糖胞苷三磷酸((F-ara-ATP)。该代谢产物抑制了DNA复制的几个关键过程,如DNA合成所需酶,比如核糖核酸还原酶,DNA引发酶,DNA聚合酶,具有3 ' - 5 '外切酶活性的脱氧核糖核酸聚合酶δ和ε和DNA连接酶[2]。通过检测ICAM - 1的表达和IL - 8释放,表明Fludarabine还可以诱导前炎症刺激单核细胞的增加[3]。Fludarabine对卵巢癌细胞系生长无影响,而它对黑色素瘤细胞系有明显的剂量依赖性抑制[4]。Fludarabine诱导显著降低STAT - 1磷酸化,但不改变JAK 2活性。磷酸Fludarabine对三种STAT蛋白磷酸化影响不显着。Fludarabine(1.5mg)在处理2天后和7天后显著降低磷酸化STAT - 1和STAT-1总蛋白量[5]。 磷酸化JAK 2在处理后第2天变化不明显,处理后第7天的JAK表达明显受抑制。Fludarabine不影响其他STAT蛋白质,特异性抑制STAT - 1激活以降低血管平滑肌细胞体外增殖。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  M4Gz[GZ2dmO2aX;uJGF{e2G7 MUKyNEDPxE1? NIfIN5UzPCCq NF7SPXdqdmirYnn0d{BmgHC{ZYPzbY9vKG:oIFnEUy=> M3;kcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OUSwO|EzLz5{NUm0NFcyOjxxYU6=
MV-4-11 M3jwOWFxd3C2b4Ppd{BCe3OjeR?= MXuyMlUh|ryP NVTjdJN1PDhiaB?= NVXUTolmcW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> NUDMXnZxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
THP-1 Mke0RZBweHSxc3nzJGF{e2G7 MkW0Nk42KM7:TR?= NVzjRYdoPDhiaB?= MXHpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= M2PoUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUGxOVg{Lz5{NUGxNVU5OzxxYU6=
MOLM 13 MkHlRZBweHSxc3nzJGF{e2G7 NF3oR4UzNjVizszN NHnqWJY1QCCq NWLpfI06cW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> M1Hz[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUGxOVg{Lz5{NUGxNVU5OzxxYU6=
KBM3/Bu2506 NXq5OJpFSXCxcITvd4l{KEG|c3H5 MVmyMlUh|ryP MX20PEBp MWrpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFzMUW4N{c,OjVzMUG1PFM9N2F-
Nalm-6 MmrES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTF6IN88US=> Mn;QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Reh MoqxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoW5TWM2OD1|MDFOwG0> NVvFNIEyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
U2937 NXPWR|NqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HPN2lEPTB;MU[g{txO MmjnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Mec-1 M4fwbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1K0cmlEPTExvK61NFAh|ryP M1n6V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
RPMI-8226 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoHOTWM2OD13MECg{txO NVXCfpBGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
Molt-4 NYnGO3VqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYexd|dNUUN3ME2xPFAh|ryP NVj2WVQ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
Nalm-6-FluR NEP5TXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF65W5NKSzVyPUK1NEDPxE1? M4HtVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
Raji  Mn\6SpVv[3Srb36gRZN{[Xl? MVmzxsDPxE1? NHfIdXozPC92OD:3NkBp Mn\kbY5lfWOnczDhZ4N2dXWuYYTpc45{KG:oIIC1N{wheDZ|IHHu[EBxPzQEoB?= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl2ME[5OUc,OjR7NEC2PVU9N2F-
PBMC MmTpSpVv[3Srb36gRZN{[Xl? Ml\3OVAwOTByIN88US=> NXrJTY1QOjRiaB?= NUDKOIliTE2VTx?= MmTobY5pcWKrdIOgV3RCXDFicHjvd5Bpd3K7bHH0bY9v MnmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
MDA-231 NHrx[FRHfW6ldHnvckBCe3OjeR?= M1TBSVExOCEQvF2= NHrvbnQzPCCq NYrIUphxTE2VTx?= MmfK[IVkemWjc3XzJGlFVyCneIDy[ZN{cW:w NHnydYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
624.38mel  NX23NG44TnWwY4Tpc44hSXO|YYm= MnjjOVAh|ryP MnH3NlQhcA>? MYrEUXNQ NH6zZpZl\WO{ZXHz[ZMhUUSRIHX4dJJme3Orb36= NYjnUHRSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
MDA-231 MknxSpVv[3Srb36gRZN{[Xl? MoPLOVAuOjByIN88US=> MYKyOEBp NHexcXZFVVOR Ml[2bY5pcWKrdIOgTWRQKGGldHn2bZR6KGmwZHXw[Y5l\W62bImgc4YhdVKQQTDs[ZZmdHN? NFrOb|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
624.38mel  MkXQSpVv[3Srb36gRZN{[Xl? NXTxTolDPTBvMkCwJO69VQ>? M3nRflI1KGh? NF3HRXZFVVOR M2LsWolvcGmkaYTzJGlFVyCjY4Tpeol1gSCrbnTldIVv\GWwdHz5JI9nKG2UTlGgcIV3\Wy| M4WwR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUGxPFczLz5{NEmxNVg4OjxxYU6=
HMECs NWXnN5lWTnWwY4Tpc44hSXO|YYm= NFO1XlQyODEEoN88UeKh Mn7PN|bDqGh? NXHQfHpzcW6qaXLpeJMhUU[QzsRCpIFv\CCOUGOgbY5lfWOnZDDTWGFVOSCyaH;zdIhwenmuYYTpc44h[W6mIFnSSlEh\XiycnXzd4lwdg>? MmPNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUGzNlcoRjJ2MkGxN|I4RC:jPh?=
HMECs  NU\tcIdrTnWwY4Tpc44hSXO|YYm= NYfUT|BnOTBywrFOwG3DqA>? NFrZNWU{PsLiaB?= MonpbY5pcWKrdIOgTWZP|rIEoH3l[IlifGWmIIDoc5NxcG:{eXzheIlwdiCxZjDTWGFVOSCjbnSgV3RCXDNuIHL1eEBvd3Rib3[gV3RCXDJ? NVL2R3U1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNVE{OjdpPkK0NlEyOzJ5PD;hQi=>
BJAB MWPBdI9xfG:|aYOgRZN{[Xl? M33WZVXDqM7:TR?= M3nmZlI1KGh? NGP0[IZqdmS3Y3XzJINmdGxiYYDvdJRwe2m| M1P0eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEW3NVQ4Lz5{NEC1O|E1PzxxYU6=
I-83 M3\MW2Fxd3C2b4Ppd{BCe3OjeR?= NFnwcFI2yqEQvF2= NVzvWGRGOjRiaB?= MYPpcoR2[2W|IHPlcIwh[XCxcITvd4l{ MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB3N{G0O{c,OjRyNUexOFc9N2F-
NALM6 NUDjSJpwSXCxcITvd4l{KEG|c3H5 NIH6VoQ2yqEQvF2= MUiyOEBp M4G1TIlv\HWlZYOgZ4VtdCCjcH;weI9{cXN? MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB3N{G0O{c,OjRyNUexOFc9N2F-
DU-145 M{HRUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPod4tUOC1zMDFOwIcwdWx? MkDZOFghcMLi NFvKdYJqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd|NEixOUc,OjN5M{S4NVU9N2F-
Nalm-6 NFLSW5ZHfW6ldHnvckBCe3OjeR?= MUWxNOKh|ryP Mkj0NU8zNzRiaB?= NYfLSJlMcW6mdXPld{BifXSxcHjh[5k> NWPDXHljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFEzOjNpPkKzOlgyOjJ|PD;hQi=>
Reh NGiyb5lHfW6ldHnvckBCe3OjeR?= MoX3NVDDqM7:TR?= NXzLO2ZqOS9{L{SgbC=> M{nqbIlv\HWlZYOgZZV1d3CqYXf5 MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MUKyN{c,OjN4OEGyNlM9N2F-
Nalm-6 NX;PNVZyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoGyTWM2OCEkiMyxNQKBkc7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MUKyN{c,OjN4OEGyNlM9N2F-
Reh MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUm4RYdYUUN3MDFijNwyOOLCid88US=> Mn\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEGyNlMoRjJ|NkixNlI{RC:jPh?=
HEC1A Mlz3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HrbFExOC13MECg{txO MnnSNlQhcA>? NHLwU41qdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NUfjWmM4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
AN3CA MoL0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17ucVExOC13MECg{txO M{DqNVI1KGh? M2PTSolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
HEC50B M2rhPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rrZlExOC13MECg{txO NGCzPIMzPCCq NHTJOXpqdmirYnn0d{Bk\WyuIHfyc5d1cCC|bHnnbJRtgQ>? NXXBO2c6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC1A MXLBdI9xfG:|aYOgRZN{[Xl? NWSzWGhKOjBxMUCwJO69VQ>? MXSyOEBp NFLIVnlqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> M2HiSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
AN3CA Mk[2RZBweHSxc3nzJGF{e2G7 MW[yNE8yODBizszN NF\5W5kzPCCq M4jZfYlv\HWlZYOgZZBweHSxc3nzJIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
HEC50B M1HGeGFxd3C2b4Ppd{BCe3OjeR?= MYqyNE8yODBizszN NETRS4MzPCCq NXXIfpc1cW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
EHEB M2rwT2Fxd3C2b4Ppd{BCe3OjeR?= MVS0NEDPxE1? NInKW4QzPCCq NXvydJR2cW6mdXPld{BieG:ydH;zbZM> MkP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUewO|UoRjJ|NEm3NFc2RC:jPh?=
A549 M{TsS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnGzTWM2OD1zNT63xtEzNjhiwsXN M1u3RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{e3NVkzLz5{M{O3O|E6OjxxYU6=
A549 GAPDH-deficient M{\ndWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoDFTWM2OD1zOD61xtEzNjNiwsXN Mn;UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN|N{exPVIoRjJ|M{e3NVkzRC:jPh?=
CLL  M4fwO2Fxd3C2b4Ppd{BCe3OjeR?= NWfONXV4OTBizszNxsA> M2XhZVI1NTl4IHi= M2nEfYlv\HWlZYOgZZBweHSxdHnjJINmdGxiZHXheIg> MlvhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{MEe2PFYoRjJ{MkC3Olg3RC:jPh?=
MEC1 Ml7SRZBweHSxc3nzJGF{e2G7 M4LjNFExOMLizszN NWH4XpgzPzJiaB?= MXjpcoR2[2W|IHHwc5B1d3OrczDzbYdvcW[rY3HueIx6 NWPIUVZJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxN|I6PzNpPkKyNVMzQTd|PD;hQi=>
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U937  MVzBdI9xfG:|aYOgRZN{[Xl? NHTXfY0yKM7:TR?= MoPPPVYhcA>? NUm1UXc3cW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> NFjBc5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCyN|UzOyd-MkKwNlM2OjN:L3G+
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JMV-3 NEPa[5FCeG:ydH;zbZMhSXO|YYm= NUHNUoVEOTBizszNxsA> Ml73NlQhcA>? MlP1bY5lfWOnczDj[YxtKGGyb4D0c5NqeyC|bHnnbJRtgQ>? NGrrUFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[5PVM5Oyd-MkG2PVk{QDN:L3G+
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JVM-2  NUf4fG1ITnWwY4Tpc44hSXO|YYm= NVOw[IdsOzBizszN M2\HSVI1KGh? NEDVfnFl\WO{ZXHz[ZMheDJzIHX4dJJme3Orb36= MmTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzNkizPVEoRjJzMU[4N|kyRC:jPh?=
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KBM3/Bu2506 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmH4NE43KM7:TR?= NHrPXHkzPCCq Mm\KbY5kemWjc3XzJJRp\SClZXzsJIZz[WO2aX;uJIlvKFNvcHjhd4U> MnX0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7M{O1NFkoRjJyOUOzOVA6RC:jPh?=
MDA-MB-231 NF7rfWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWW3PVRvUUN3ME20MlAh|ryP M2fYVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNES3N|kxLz5{MES0O|M6ODxxYU6=
MCF-7 M{DQW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfSTWM2OD1zNT6wJO69VQ>? NIfDVXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES0O|M6OCd-MkC0OFc{QTB:L3G+
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BW-225 MmfvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{PKVmlEOjB;MT6zO{DEnzFy4pkSPOKh|ryPwrC= M1nvXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Nk[xN|gxLz5zOE[2NVM5ODxxYU6=
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CaSki  MlfjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfxWFBKSzJyPUGuN|chy5diMUFijLI4KM7:TdMg Mm\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NkGzPFAoRjF6Nk[xN|gxRC:jPh?=
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RPMI 8226 NISxU41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1fXW|I1KGh? MU\JR|UxRTFwNUVCpO69VQ>? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl5NkG4Okc,OTd7N{[xPFY9N2F-
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MM.1R Ml63S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjmXlI1QCCq NHfOZWxKSzVyPUOzMlc6KM7:TR?= M3rlRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUe2NVg3Lz5zN{m3OlE5PjxxYU6=
U937 NVvBbJpyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPCTG5OUUN3ME2zMFIxOCEEsTC1OlAhdk1? MlK4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV7M{CzOlEoRjF3OUOwN|YyRC:jPh?=
CLL5 M37U[2FvfGm2dX3vdkBie3OjeR?= NVnkdYkzPDhiaILz M4i3SWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JGNNVDViY3XscJMhcXOxbHH0[YQh\nKxbTDDUGwheGG2aXXueEBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCjbnHsfZNqeyxiRVO1NEA:KDBwMU[g{txONg>? MonlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{O3N|koRjJ2NkezO|M6RC:jPh?=
K562 MnfzR5l1d3SxeHnjbZR6KGG|c3H5 M1OyVVczKGi{cx?= NEDJTohEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBx[WOuaYThfIVtNXKnc3nzeIFvfCCNNU[yJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkK2JO69VS5? NWTEOIU6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NFU3PTZpPkKwOlA2PjV4PD;hQi=>
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CLL4 MnG1RY51cXS3bX;yJIF{e2G7 M1PWZlQ5KGi{cx?= NHrhSIxCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBEVEx2IHPlcIx{KGm|b3zheIVlKG[{b32gR2xNKHCjdHnlcpQh[XO|ZYPz[YQh[XNiY3XscEB3cWGkaXzpeJkh[W[2ZYKgOFghcHK|IHL5JGZCS1NiYX7hcJl{cXNuIFXDOVAhRSByLk[0JO69VS5? M3\vS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkezO|M6Lz5{NE[3N|c{QTxxYU6=
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primary CLL NEO2UmdEgXSxdH;4bYNqfHliYYPzZZk> NUXsSXhnS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heHKrbXHyfUBEVExiY3XscJMtKEyGNUCgQUAyNjFizszNMi=> Mom5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNEizPVIoRjJ3MUS4N|kzRC:jPh?=
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CLL2 NX3TTFJISW62aYT1cY9zKGG|c3H5 NGTwO4o1QCCqcoO= M2XXdWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JGNNVDJiY3XscJMhcXOxbHH0[YQh\nKxbTDDUGwheGG2aXXueEBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCjbnHsfZNqeyxiRVO1NEA:KDJwNk[g{txONg>? M2PjflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkezO|M6Lz5{NE[3N|c{QTxxYU6=
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SK-N-MC NGfURWJyUFSVIHHzd4F6 MknKdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= NFHnRVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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NB1643 NHzxO4hyUFSVIHHzd4F6 MoHXdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIyPjR|IHPlcIx{ NGf1Z449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
procaspase-9 / procaspase-3; 

PubMed: 27223263     


Procaspase-9 and procaspase-3 were analyzed by Western blot in CLL cells from three patients after a 48 h-incubation in the absence or presence of 3 μM aphidicolin (APC) with or without fludarabine at 0.3 and 1 μM. β-Actin served as a loading control.

p-p53 / p53; 

PubMed: 27223263     


(A–B) CLL cells from 3 different patients were incubated for 24 h with fludarabine at the indicated concentrations in the absence or presence of 3 μM aphidicolin (APC). Phosphorylation of p53 at Ser-15 and total p53 were analyzed by Western blot. Data sho䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒

STAT1; 

PubMed: 26677135     


Representative western blot analysis of STAT1 in RAW 264.7 cells treated with STAT1 inhibitor fludarabine (Flu) for 24 h.

27223263 26677135
Immunofluorescence
α-SMA / Vimentin; 

PubMed: 28322315     


FLU (50 μM) reduces the expression of α-SMA and Vimentin protein in primary mouse activated HSCs (Hepatic Stellate Cells).

28322315
Growth inhibition assay
Cell viability ; 

PubMed: 24956101     


CLL cells RPMI/0.1% FBS were cultured on 0.5% BSA or 150 nM MMP-9 for 1 h prior to adding the indicated concentrations of fludarabine (Fluda). After 48 h (Fluda) cell viability was determined by flow cytometry using FITC-Annexin V and PI.

24956101
体内研究 PBS对照组中肿瘤细胞增长迅速,25天内扩增为10倍。而用40mg/kgFludarabine处理的肿瘤扩增不超过5倍。40mg/kgFludarabine对RPMI 8226细胞有明显的抗肿瘤效果。RPMI 8226处理肿瘤细胞十天后增加凋亡细胞核数量。[1] Fludarabine可在免疫缺陷小鼠中有效抑制RPMI 8226细胞的骨髓转移。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: 地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系
  • Concentrations: 2 μg/Ml
  • Incubation Time: 24 小时
  • Method:

    对地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系以5 × 105细胞密度铺板,实验组均包括氟达拉滨处理和空白对照组。处理后细胞用PBS清洗两次然后用70%冰乙醇固定,离心后用含100μg/mL核糖核酸酶PBS重悬浮,37ºC孵育30分钟。样品重悬于25μg/mL碘化吡啶中,用流式细胞仪检测。根据产品说明用Annexin V-FITC试剂盒检测细胞凋亡。利用TUNEL(末端脱氧核苷酸转移酶介导脱氧核苷酸缺口末端标记)法和原位细胞凋亡检测试剂盒处理细胞,最后用流式细胞仪分析。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带RPMI 8226肿瘤细胞的免疫缺陷型小鼠
  • Dosages: 40 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 57 mg/mL (199.83 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
8 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 285.23
化学式

C10H12FN5O4

CAS号 21679-14-1
储存条件 粉状
溶于溶剂
别名 FaraA, Fludarabinum

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04526795 Recruiting Drug: Cytarabine|Drug: Fludarabine|Drug: Pegcrisantaspase Blast Phase Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent Acute Biphenotypic Leukemia|Recurrent Acute Lymphoblastic Leukemia|Recurrent Acute Myeloid Leukemia|Recurrent Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent T-Cell Prolymphocytic Leukemia|Refractory Acute Biphenotypic Leukemia|Refractory Acute Lymphoblastic Leukemia|Refractory Acute Myeloid Leukemia|Refractory Chronic Myelogenous Leukemia BCR-ABL1 Positive|Refractory T-Cell Prolymphocytic Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) April 9 2021 Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    how to re-suspend and deliver the inhibitor for in vivo experiments?

  • 回答:

    For S1491, Fludarabine, we tested a vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W that you can resuspend the compound in at up to 30mg/ml. It's a suspension and can only be given via oral gavage.

STAT Signaling Pathway Map

相关STAT产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID