Fludarabine (NSC 118218)

目录号:S1491 别名: FaraA, Fludarabinum 中文名称:氟达拉滨

仅限科研使用

Fludarabine (NSC 118218, FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。

Fludarabine (NSC 118218) Chemical Structure

CAS: 21679-14-1

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1032.75 现货
RMB 810.59 现货
RMB 2525.07 现货
RMB 7289.1 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Fludarabine (NSC 118218)发表文献135篇:

产品安全说明书

STAT抑制剂选择性比较

相关STAT产品

生物活性

产品描述 Fludarabine (NSC 118218, FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。
靶点
STAT1 [4]
(Vascular smooth muscle cells)
体外研究

Fludarabine有效抑制RPMI8226细胞增殖,呈时间浓度依赖性。Fludarabine处理MM.1S和MM.1R细胞48小时的IC50分别为13.48μg/mL和33.79μg/mL。而Fludarabine处理U266细胞48小时的IC 50为222.2μg/mL。Fludarabine处理可增加处于细胞周期G 1期的细胞数量,并伴随着处于细胞周期S期细胞数量的减少,对细胞周期的影响呈时间依赖性。Fludarabine在MM细胞中诱导细胞周期阻滞和细胞凋亡。Fludarabine时间依赖性的诱导caspase,-8,- 9和- 3,- 7的剪切,继而诱导PARP的剪切 。氟达拉滨以时间梯度增加Bax基因表达,而Bak基因表达量不变。RPMI 8226细胞用Fludarabine处理12小时,处理后细胞膜电位降低,61.05%的细胞表达低荧光罗丹明123,而未处理的对照组细胞仅为8.62%[1]。为提高溶解度,Fludarabine可制为一磷酸盐形式(F-ara-AMP, fudarabine),在静脉滴注后即可即时、定量的从母核上去磷酸化。细胞内重磷酸化修饰导致Fludarabine-阿糖胞苷三磷酸((F-ara-ATP)。该代谢产物抑制了DNA复制的几个关键过程,如DNA合成所需酶,比如核糖核酸还原酶,DNA引发酶,DNA聚合酶,具有3 ' - 5 '外切酶活性的脱氧核糖核酸聚合酶δ和ε和DNA连接酶[2]。通过检测ICAM - 1的表达和IL - 8释放,表明Fludarabine还可以诱导前炎症刺激单核细胞的增加[3]。Fludarabine对卵巢癌细胞系生长无影响,而它对黑色素瘤细胞系有明显的剂量依赖性抑制[4]。Fludarabine诱导显著降低STAT - 1磷酸化,但不改变JAK 2活性。磷酸Fludarabine对三种STAT蛋白磷酸化影响不显着。Fludarabine(1.5mg)在处理2天后和7天后显著降低磷酸化STAT - 1和STAT-1总蛋白量[5]。 磷酸化JAK 2在处理后第2天变化不明显,处理后第7天的JAK表达明显受抑制。Fludarabine不影响其他STAT蛋白质,特异性抑制STAT - 1激活以降低血管平滑肌细胞体外增殖。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  MWDGeY5kfGmxbjDBd5NigQ>? MnvRNlAh|ryP NYTNbZlXOjRiaB?= NYr6XIZYcW6qaXLpeJMh\XiycnXzd4lwdiCxZjDJSG8> NWPBSIhzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OFA4OTJpPkK1PVQxPzF{PD;hQi=>
MV-4-11 MoTPRZBweHSxc3nzJGF{e2G7 NH63cJQzNjVizszN MX20PEBp NGXseY5qdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFzMUW4N{c,OjVzMUG1PFM9N2F-
THP-1 MnziRZBweHSxc3nzJGF{e2G7 MoTyNk42KM7:TR?= MmXQOFghcA>? MmfTbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= NVHu[JFtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
MOLM 13 MofNRZBweHSxc3nzJGF{e2G7 M2qybVIvPSEQvF2= MWK0PEBp NEnS[|lqdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTFzMUW4N{c,OjVzMUG1PFM9N2F-
KBM3/Bu2506 NVHSVXR{SXCxcITvd4l{KEG|c3H5 MWWyMlUh|ryP M2L2VVQ5KGh? M2jNOYlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 M3HrNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUGxOVg{Lz5{NUGxNVU5OzxxYU6=
Nalm-6 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTMTWM2OD1zODFOwG0> NGCxeGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
Reh MlrwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4S5RWlEPTB;M{Cg{txO NXXmNIt7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
U2937 NGPvcppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnXNTWM2OD1zNjFOwG0> M3LLXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
Mec-1 NXrsNFRuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHX2S2lKSzVy78{eOVAxKM7:TR?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
RPMI-8226 NUewb2QxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvUSmtKSzVyPUWwNEDPxE1? M3\vV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
Molt-4 M1Lnbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnjoTWM2OD1zOECg{txO NIrwWXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
Nalm-6-FluR NV;ZfZJ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjlNGpKSzVyPUK1NEDPxE1? M4XDVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
Raji  MnXBSpVv[3Srb36gRZN{[Xl? NYjmO5lCO8LizszN MlW0NlQwPDhxN{KgbC=> MVnpcoR2[2W|IHHjZ5VufWyjdHnvcpMhd2ZicEWzMEBxPjNiYX7kJJA4O8Li M2TCRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUSwOlk2Lz5{NEm0NFY6PTxxYU6=
PBMC NYX1cVdoTnWwY4Tpc44hSXO|YYm= Ml\0OVAwOTByIN88US=> MofyNlQhcA>? M1rQWWROW09? Mki2bY5pcWKrdIOgV3RCXDFicHjvd5Bpd3K7bHH0bY9v MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlzMUi3Nkc,OjR7MUG4O|I9N2F-
MDA-231 NV3oeZpyTnWwY4Tpc44hSXO|YYm= MUCxNFAh|ryP M3q1c|I1KGh? MmnzSG1UVw>? NUPTXXVD\GWlcnXhd4V{KEmGTzDlfJBz\XO|aX;u NVm4eYQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
624.38mel  M3XyRmZ2dmO2aX;uJGF{e2G7 NXP1bXlSPTBizszN MYqyOEBp MkH0SG1UVw>? NF[5Wo5l\WO{ZXHz[ZMhUUSRIHX4dJJme3Orb36= NUjQNXdlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
MDA-231 MXXGeY5kfGmxbjDBd5NigQ>? MYO1NE0zODBizszN MYOyOEBp MW\EUXNQ MmXPbY5pcWKrdIOgTWRQKGGldHn2bZR6KGmwZHXw[Y5l\W62bImgc4YhdVKQQTDs[ZZmdHN? NGroOnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
624.38mel  MlPXSpVv[3Srb36gRZN{[Xl? NHzCdJE2OC1{MECg{txO MYCyOEBp MkXMSG1UVw>? Moe1bY5pcWKrdIOgTWRQKGGldHn2bZR6KGmwZHXw[Y5l\W62bImgc4YhdVKQQTDs[ZZmdHN? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlzMUi3Nkc,OjR7MUG4O|I9N2F-
HMECs MYXGeY5kfGmxbjDBd5NigQ>? MYCxNFDDqM7:TdMg NWLZPXpIOzcEoHi= NFTQUpBqdmirYnn0d{BKTk8Qs9MgZY5lKEySUzDpcoR2[2WmIGPURXQyKHCqb4PwbI9zgWyjdHnvckBidmRiSWLGNUBmgHC{ZYPzbY9v MnfIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUGzNlcoRjJ2MkGxN|I4RC:jPh?=
HMECs  MXHGeY5kfGmxbjDBd5NigQ>? MXqxNFDDqM7:TdMg MWGzOuKhcA>? MWXpcohq[mm2czDJSm7PucLibXXkbYF1\WRicHjvd5Bpd3K7bHH0bY9vKG:oIGPURXQyKGGwZDDTWGFVOyxiYoX0JI5wfCCxZjDTWGFVOg>? Ml7KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUGzNlcoRjJ2MkGxN|I4RC:jPh?=
BJAB MVLBdI9xfG:|aYOgRZN{[Xl? M3zWdFXDqM7:TR?= M1;rbFI1KGh? M1H2Xolv\HWlZYOgZ4VtdCCjcH;weI9{cXN? NVn2XFZpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOVcyPDdpPkK0NFU4OTR5PD;hQi=>
I-83 MWHBdI9xfG:|aYOgRZN{[Xl? NY\jNnJUPcLizszN NGTl[YMzPCCq NH;me3hqdmS3Y3XzJINmdGxiYYDvdJRwe2m| M{fx[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEW3NVQ4Lz5{NEC1O|E1PzxxYU6=
NALM6 NEPKOG5CeG:ydH;zbZMhSXO|YYm= M3znW|XDqM7:TR?= NXX1d2JGOjRiaB?= MnTNbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? Mnr6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNUexOFcoRjJ2MEW3NVQ4RC:jPh?=
DU-145 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHmNE0yOCEQvHevcYw> NGO4SG81QCCqwrC= NYXWO4RJcW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? NYrtRWtVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3N|Q5OTVpPkKzO|M1QDF3PD;hQi=>
Nalm-6 NIrtd|dHfW6ldHnvckBCe3OjeR?= NHGxfWEyOMLizszN NXfiVXRVOS9{L{SgbC=> NIHmN4JqdmS3Y3XzJIF2fG:yaHHnfS=> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MUKyN{c,OjN4OEGyNlM9N2F-
Reh NWnzR3FzTnWwY4Tpc44hSXO|YYm= NXzENJA1OTEEoN88US=> MYexM|IwPCCq M2DVTolv\HWlZYOgZZV1d3CqYXf5 NFLEWJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NVIzOyd-MkO2PFEzOjN:L3G+
Nalm-6 M1;1U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxKOLKvEGw5qCK|ryP NW\QRWI5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFEzOjNpPkKzOlgyOjJ|PD;hQi=>
Reh M1nndmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHLLfY9KSzVyIPMIwFEx6oDLzszN MlW0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEGyNlMoRjJ|NkixNlI{RC:jPh?=
HEC1A NIS3dYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mki2NVAxNTVyMDFOwG0> NY\BeYl3OjRiaB?= NES2SJNqdmirYnn0d{Bk\WyuIHfyc5d1cCCrbjDhJIRwe2VvZHXw[Y5l\W62IH3hco5meg>? NXvZTmR2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
AN3CA M{f2Wmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUGxNFAuPTByIN88US=> MoHBNlQhcA>? MVXpcohq[mm2czDj[YxtKGe{b4f0bEBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> NHLCfnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
HEC50B M2\qRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPEXWMyODBvNUCwJO69VQ>? MYeyOEBp M3;HSIlvcGmkaYTzJINmdGxiZ4Lve5RpKHOuaXfoeIx6 MlPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3OUW2PVcoRjJ|NUm1Olk4RC:jPh?=
HEC1A MUnBdI9xfG:|aYOgRZN{[Xl? MUmyNE8yODBizszN M2\tWVI1KGh? MmK3bY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NYn0W5NmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
AN3CA M1fyRWFxd3C2b4Ppd{BCe3OjeR?= M4HIUFIxNzFyMDFOwG0> MlSyNlQhcA>? NWm2OoI1cW6mdXPld{BieG:ydH;zbZMhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NU\vOFcxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC50B M3PVfWFxd3C2b4Ppd{BCe3OjeR?= NX3DblFmOjBxMUCwJO69VQ>? MWCyOEBp NYrubms2cW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> M{XqV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
EHEB NUTwV4FQSXCxcITvd4l{KEG|c3H5 MWC0NEDPxE1? MX[yOEBp NYGzNFQ3cW6mdXPld{BieG:ydH;zbZM> NX;oUG9vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVcxPzVpPkKzOFk4ODd3PD;hQi=>
A549 M2LmZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWK3VIJ3UUN3ME2xOU44yrF{LkigxtVO NHK0OY09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O3O|E6Oid-MkOzO|cyQTJ:L3G+
A549 GAPDH-deficient M{fQW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrLfG9GUUN3ME2xPE42yrF{LkOgxtVO NFnyUnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O3O|E6Oid-MkOzO|cyQTJ:L3G+
CLL  M3PlZ2Fxd3C2b4Ppd{BCe3OjeR?= NYTkNHlHOTBizszNxsA> NGLVeGMzPC17NjDo M124S4lv\HWlZYOgZZBweHSxdHnjJINmdGxiZHXheIg> NYn5[pZ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNFc3QDZpPkKyNlA4Pjh4PD;hQi=>
MEC1 MlXYRZBweHSxc3nzJGF{e2G7 M4rGeFExOMLizszN MofBO|IhcA>? NWnlV2lPcW6mdXPld{BieG:ydH;zbZMhe2mpbnnmbYNidnSueR?= NGTpOWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkGzNlk4Oyd-MkKxN|I6PzN:L3G+
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K562 MUDDfZRwfG:6aXPpeJkh[XO|YYm= MkHzO|IhcHK| NIHkZ|NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBx[WOuaYThfIVtNXKnc3nzeIFvfCCNNU[yJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkK2JO69VS5? NVW2VZZLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NFU3PTZpPkKwOlA2PjV4PD;hQi=>
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LAN-5 MYXxTHRUKGG|c3H5 NV:wXYE5eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFzBUk02KGOnbHzz NHjwdoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh18 M1;YdZFJXFNiYYPzZZk> M4LUXJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy| Ml;JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 MWLxTHRUKGG|c3H5 NXPOeXVHeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| Mn;JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC MXLxTHRUKGG|c3H5 MoPBdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2suVi2PQzDj[Yxtew>? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 M{PsXpFJXFNiYYPzZZk> M{mwXpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNLNUeETUKgZ4VtdHN? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
TC32 NVPYZ21VeUiWUzDhd5NigQ>? M33VdZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> NXfReXlURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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Saos-2 MXnxTHRUKGG|c3H5 M1jEb5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNid3NvMjDj[Yxtew>? M2TVXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 NFuy[ZJyUFSVIHHzd4F6 MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBQenSqb3fvcoFtKDOGII\pZYJqdGm2eTDzZ5Jm\W5iZn;yJHNLNUeETUKgZ4VtdHN? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
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Assay
Methods Test Index PMID
Western blot procaspase-9 / procaspase-3 p-p53 / p53 STAT1 27223263 26677135
Immunofluorescence α-SMA / Vimentin 28322315
Growth inhibition assay Cell viability 24956101
体内研究 PBS对照组中肿瘤细胞增长迅速,25天内扩增为10倍。而用40mg/kgFludarabine处理的肿瘤扩增不超过5倍。40mg/kgFludarabine对RPMI 8226细胞有明显的抗肿瘤效果。RPMI 8226处理肿瘤细胞十天后增加凋亡细胞核数量。[1] Fludarabine可在免疫缺陷小鼠中有效抑制RPMI 8226细胞的骨髓转移。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

  • Cell lines: 地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系
  • Concentrations: 2 μg/Ml
  • Incubation Time: 24 小时
  • Method:

    对地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系以5 × 105细胞密度铺板,实验组均包括氟达拉滨处理和空白对照组。处理后细胞用PBS清洗两次然后用70%冰乙醇固定,离心后用含100μg/mL核糖核酸酶PBS重悬浮,37ºC孵育30分钟。样品重悬于25μg/mL碘化吡啶中,用流式细胞仪检测。根据产品说明用Annexin V-FITC试剂盒检测细胞凋亡。利用TUNEL(末端脱氧核苷酸转移酶介导脱氧核苷酸缺口末端标记)法和原位细胞凋亡检测试剂盒处理细胞,最后用流式细胞仪分析。

动物实验:

[1]

  • Animal Models: 携带RPMI 8226肿瘤细胞的免疫缺陷型小鼠
  • Dosages: 40 mg/kg
  • Administration: 腹腔注射

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O

8 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 285.23
化学式

C10H12FN5O4

CAS号 21679-14-1
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05390814 Not yet recruiting Drug: PET-MRI Primary Central Nervous System Lymphoma Assistance Publique - Hôpitaux de Paris June 15 2022 Phase 1
NCT05290662 Recruiting Drug: oNKord®|Drug: Cyclophosphamide/Fludarabine (Cy/Flu) AML Glycostem Therapeutics BV June 2022 --
NCT05201183 Recruiting Combination Product: Fludarabine + Total Marrow Irradiation Acute Myeloid Leukemia|Chronic Myeloid Leukemia|Acute Lymphocytic Leukemia|Myelodysplastic Syndromes Naoyuki G. Saito M.D. Ph.D.|Indiana University May 17 2022 Phase 1|Phase 2
NCT03832127 Not yet recruiting Drug: 18F-Fludarabine Myeloma Nantes University Hospital|Cyceron April 1 2022 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

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常见问题及建议解决方法

问题 1:
how to re-suspend and deliver the inhibitor for in vivo experiments?

回答:
For S1491, Fludarabine, we tested a vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W that you can resuspend the compound in at up to 30mg/ml. It's a suspension and can only be given via oral gavage.

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