Fludarabine (NSC 118218)

For research use only. Not for use in humans.

目录号:S1491 别名: FaraA, Fludarabinum 中文名称:氟达拉滨

Fludarabine (NSC 118218) Chemical Structure

CAS No. 21679-14-1

Fludarabine (NSC 118218, FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1032.75 现货
RMB 810.59 现货
RMB 2525.07 现货
RMB 7289.1 现货
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客户使用Selleck生产的Fludarabine (NSC 118218)发表文献126篇:

产品安全说明书

STAT抑制剂选择性比较

生物活性

产品描述 Fludarabine (NSC 118218, FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。
靶点
STAT1 [4]
(Vascular smooth muscle cells)
体外研究

Fludarabine有效抑制RPMI8226细胞增殖,呈时间浓度依赖性。Fludarabine处理MM.1S和MM.1R细胞48小时的IC50分别为13.48μg/mL和33.79μg/mL。而Fludarabine处理U266细胞48小时的IC 50为222.2μg/mL。Fludarabine处理可增加处于细胞周期G 1期的细胞数量,并伴随着处于细胞周期S期细胞数量的减少,对细胞周期的影响呈时间依赖性。Fludarabine在MM细胞中诱导细胞周期阻滞和细胞凋亡。Fludarabine时间依赖性的诱导caspase,-8,- 9和- 3,- 7的剪切,继而诱导PARP的剪切 。氟达拉滨以时间梯度增加Bax基因表达,而Bak基因表达量不变。RPMI 8226细胞用Fludarabine处理12小时,处理后细胞膜电位降低,61.05%的细胞表达低荧光罗丹明123,而未处理的对照组细胞仅为8.62%[1]。为提高溶解度,Fludarabine可制为一磷酸盐形式(F-ara-AMP, fudarabine),在静脉滴注后即可即时、定量的从母核上去磷酸化。细胞内重磷酸化修饰导致Fludarabine-阿糖胞苷三磷酸((F-ara-ATP)。该代谢产物抑制了DNA复制的几个关键过程,如DNA合成所需酶,比如核糖核酸还原酶,DNA引发酶,DNA聚合酶,具有3 ' - 5 '外切酶活性的脱氧核糖核酸聚合酶δ和ε和DNA连接酶[2]。通过检测ICAM - 1的表达和IL - 8释放,表明Fludarabine还可以诱导前炎症刺激单核细胞的增加[3]。Fludarabine对卵巢癌细胞系生长无影响,而它对黑色素瘤细胞系有明显的剂量依赖性抑制[4]。Fludarabine诱导显著降低STAT - 1磷酸化,但不改变JAK 2活性。磷酸Fludarabine对三种STAT蛋白磷酸化影响不显着。Fludarabine(1.5mg)在处理2天后和7天后显著降低磷酸化STAT - 1和STAT-1总蛋白量[5]。 磷酸化JAK 2在处理后第2天变化不明显,处理后第7天的JAK表达明显受抑制。Fludarabine不影响其他STAT蛋白质,特异性抑制STAT - 1激活以降低血管平滑肌细胞体外增殖。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  NYjSfJY2TnWwY4Tpc44hSXO|YYm= MmfhNlAh|ryP M2PBTlI1KGh? MXHpcohq[mm2czDlfJBz\XO|aX;uJI9nKEmGTx?= NEPpTmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm0NFcyOid-MkW5OFA4OTJ:L3G+
MV-4-11 NIq2Nm9CeG:ydH;zbZMhSXO|YYm= MVKyMlUh|ryP M4jJUVQ5KGh? M3H4PIlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 NFrESog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGxNVU5Oyd-MkWxNVE2QDN:L3G+
THP-1 MkfCRZBweHSxc3nzJGF{e2G7 M1L1[VIvPSEQvF2= M4Xue|Q5KGh? MVXpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= M4TFS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUGxOVg{Lz5{NUGxNVU5OzxxYU6=
MOLM 13 NUHTNlBGSXCxcITvd4l{KEG|c3H5 NUTHU2s6Oi53IN88US=> NEDVe4M1QCCq MVjpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= Ml[xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMUG1PFMoRjJ3MUGxOVg{RC:jPh?=
KBM3/Bu2506 NG\FcXRCeG:ydH;zbZMhSXO|YYm= M1HzfVIvPSEQvF2= MXK0PEBp NHPDbnlqdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> NUTnU5lQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
Nalm-6 NHzafYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXjUoFKSzVyPUG4JO69VQ>? M3;B[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
Reh MmTCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LaOWlEPTB;M{Cg{txO MnPRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
U2937 NUG1bmhWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XPXGlEPTB;MU[g{txO MljJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Mec-1 NGXEbIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1vtSmlEPTExvK61NFAh|ryP NGC2SlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
RPMI-8226 M2f1cmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn:1TWM2OD13MECg{txO M3PNZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3ME[xNVAyLz5{NUC2NVExOTxxYU6=
Molt-4 M{LsVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIWxbFZKSzVyPUG4NEDPxE1? NFTF[2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
Nalm-6-FluR MnnBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTJ3MDFOwG0> NXfmO45ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
Raji  NHjT[5dHfW6ldHnvckBCe3OjeR?= M3jLW|PDqM7:TR?= NVzGNGhmOjRxNEivO|IhcA>? MX\pcoR2[2W|IHHjZ5VufWyjdHnvcpMhd2ZicEWzMEBxPjNiYX7kJJA4O8Li NX7RVohRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OFA3QTVpPkK0PVQxPjl3PD;hQi=>
PBMC NXjEV4F{TnWwY4Tpc44hSXO|YYm= MoXuOVAwOTByIN88US=> NV7uOXFmOjRiaB?= MVrEUXNQ NI\jOFlqdmirYnn0d{BUXEGWMTDwbI9{eGixconsZZRqd25? MoPjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
MDA-231 MkCySpVv[3Srb36gRZN{[Xl? M4TV[lExOCEQvF2= M3nMbFI1KGh? MUTEUXNQ M3zHUIRm[3KnYYPld{BKTE9iZYjwdoV{e2mxbh?= MlH0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
624.38mel  MUfGeY5kfGmxbjDBd5NigQ>? MW[1NEDPxE1? NVqyUXZPOjRiaB?= MXjEUXNQ MU\k[YNz\WG|ZYOgTWRQKGW6cILld5Nqd25? Mon0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
MDA-231 MUDGeY5kfGmxbjDBd5NigQ>? MnL4OVAuOjByIN88US=> M1PsUFI1KGh? M4DOcWROW09? MmLUbY5pcWKrdIOgTWRQKGGldHn2bZR6KGmwZHXw[Y5l\W62bImgc4YhdVKQQTDs[ZZmdHN? NGLoblk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
624.38mel  NVPlW2x6TnWwY4Tpc44hSXO|YYm= NFzjN5k2OC1{MECg{txO MVSyOEBp NFG1e|BFVVOR NUjKdppScW6qaXLpeJMhUUSRIHHjeIl3cXS7IHnu[IVx\W6mZX70cJkhd2ZibWLORUBt\X[nbIO= NUXxe4VbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
HMECs NYTkfWl3TnWwY4Tpc44hSXO|YYm= MXKxNFDDqM7:TdMg NX;0eYRMOzcEoHi= MWTpcohq[mm2czDJSm7Pu8LiYX7kJGxRWyCrbnT1Z4VlKFOWQWSxJJBpd3OyaH;yfYxifGmxbjDhcoQhUVKIMTDlfJBz\XO|aX;u MmTLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUGzNlcoRjJ2MkGxN|I4RC:jPh?=
HMECs  NGLRPXRHfW6ldHnvckBCe3OjeR?= MlLkNVAxyqEQvF5CpC=> NFPPZo8{PsLiaB?= MknXbY5pcWKrdIOgTWZP|rIEoH3l[IlifGWmIIDoc5NxcG:{eXzheIlwdiCxZjDTWGFVOSCjbnSgV3RCXDNuIHL1eEBvd3Rib3[gV3RCXDJ? MoXTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUGzNlcoRjJ2MkGxN|I4RC:jPh?=
BJAB M4\Ib2Fxd3C2b4Ppd{BCe3OjeR?= MYm1xsDPxE1? MYWyOEBp NEm3eHJqdmS3Y3XzJINmdGxiYYDvdJRwe2m| M1fjRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEW3NVQ4Lz5{NEC1O|E1PzxxYU6=
I-83 NWPleohuSXCxcITvd4l{KEG|c3H5 NUjSUm5PPcLizszN MUGyOEBp MnnSbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? NYH5NXRjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOVcyPDdpPkK0NFU4OTR5PD;hQi=>
NALM6 NHT5bYFCeG:ydH;zbZMhSXO|YYm= NVrPS|VtPcLizszN Mlr3NlQhcA>? M2e5Volv\HWlZYOgZ4VtdCCjcH;weI9{cXN? NWfwdYc5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOVcyPDdpPkK0NFU4OTR5PD;hQi=>
DU-145 Mn7nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmGxNE0yOCEQvHevcYw> MUC0PEBpyqB? M3P2RolvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz M3jVe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{O0PFE2Lz5{M{ezOFgyPTxxYU6=
Nalm-6 NFrIcZdHfW6ldHnvckBCe3OjeR?= M17GeVExyqEQvF2= NHXGbpEyNzJxNDDo NFy1R25qdmS3Y3XzJIF2fG:yaHHnfS=> NEXzdHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NVIzOyd-MkO2PFEzOjN:L3G+
Reh Mn7uSpVv[3Srb36gRZN{[Xl? MlzONVDDqM7:TR?= MVSxM|IwPCCq NVLYWXNtcW6mdXPld{BifXSxcHjh[5k> NHnpXIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NVIzOyd-MkO2PFEzOjN:L3G+
Nalm-6 MnnQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPteYx1UUN3MDFijNwyOOLCid88US=> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MUKyN{c,OjN4OEGyNlM9N2F-
Reh M4HBPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvsN5R2UUN3MDFijNwyOOLCid88US=> NUjpRVNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2PFEzOjNpPkKzOlgyOjJ|PD;hQi=>
HEC1A M3jhcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHBc5ExOTByLUWwNEDPxE1? NELINmYzPCCq MlPXbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NULlNZY{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
AN3CA NHvv[JdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEH0WVMyODBvNUCwJO69VQ>? NE\ab3UzPCCq NWfBXmwycW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M4XFWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
HEC50B MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NISwbXoyODBvNUCwJO69VQ>? NF\DdWczPCCq NGjpSXJqdmirYnn0d{Bk\WyuIHfyc5d1cCC|bHnnbJRtgQ>? M3LzVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
HEC1A M1iyUmFxd3C2b4Ppd{BCe3OjeR?= M3vYN|IxNzFyMDFOwG0> NHT0PFMzPCCq NWPEdWR7cW6mdXPld{BieG:ydH;zbZMhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NFXr[Hc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
AN3CA MXLBdI9xfG:|aYOgRZN{[Xl? NITuVYIzOC9zMECg{txO NYH3VXlWOjRiaB?= Ml7ObY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= NXuwSmtJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC50B M4XZbWFxd3C2b4Ppd{BCe3OjeR?= MYOyNE8yODBizszN NXnHO|dPOjRiaB?= MnjNbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= NFfjOXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
EHEB MV\BdI9xfG:|aYOgRZN{[Xl? Ml7SOFAh|ryP NHHDWnozPCCq NEDkTIFqdmS3Y3XzJIFxd3C2b4Ppdy=> NWnTR5hVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVcxPzVpPkKzOFk4ODd3PD;hQi=>
A549 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHoTWM2OD1zNT63xtEzNjhiwsXN NYLaV|E6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzO|cyQTJpPkKzN|c4OTl{PD;hQi=>
A549 GAPDH-deficient MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDyTWM2OD1zOD61xtEzNjNiwsXN M4DpTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{e3NVkzLz5{M{O3O|E6OjxxYU6=
CLL  MoTrRZBweHSxc3nzJGF{e2G7 NVTpOppROTBizszNxsA> MkD3NlQuQTZiaB?= M3\kXIlv\HWlZYOgZZBweHSxdHnjJINmdGxiZHXheIg> M13zS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkC3Olg3Lz5{MkKwO|Y5PjxxYU6=
MEC1 NWTLSZlKSXCxcITvd4l{KEG|c3H5 MV:xNFDDqM7:TR?= M373eFczKGh? NULsR25ocW6mdXPld{BieG:ydH;zbZMhe2mpbnnmbYNidnSueR?= MkLSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzM{K5O|MoRjJ{MUOyPVc{RC:jPh?=
U937  NI[1fWdCeG:ydH;zbZMhSXO|YYm= NEnmVVExNjhizszN Mn[2OE01QCCq NULsb5pGcW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB5NEewNEc,OjJyN{S3NFA9N2F-
U937  M1vxUWFxd3C2b4Ppd{BCe3OjeR?= NHzJTFEyKM7:TR?= MU[5OkBp NGm3NWZqdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{M{WyN{c,OjJyMkO1NlM9N2F-
Daudi NIrnT5hCeG:ydH;zbZMhSXO|YYm= MoLtNlAh|ryP M1O1UVk3KGh? M{D2WYlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{M{WyN{c,OjJyMkO1NlM9N2F-
J45.01 NFzNWmhCeG:ydH;zbZMhSXO|YYm= M{Hk[lEh|ryP MknUPVYhcA>? MUPpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= NVrrcXdZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwNlM2OjNpPkKyNFI{PTJ|PD;hQi=>
RPMI 8226 MnLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2T3UmlEPTB;MkWuPeKhyrIEoEOuO{DPxE1? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OEK2OEc,OjF7NEiyOlQ9N2F-
CEM NXy5TXFiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWPJR|UxRTJwNNMgxtHDqDBwNDFOwG0> NYPWZXhyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5OFgzPjRpPkKxPVQ5OjZ2PD;hQi=>
Raji M1j6c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkTOTWM2OD1yLkS3xsDDucLiMD6wOEDPxE1? NGi3Uoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm0PFI3PCd-MkG5OFgzPjR:L3G+
U937 MmLPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTBwMkVCpOKyyqByLkC0JO69VQ>? M2LENFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUS4NlY1Lz5{MUm0PFI3PDxxYU6=
K562 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XKSmlEPTB;MD60OOKhyrIEoECuNFUh|ryP MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OEK2OEc,OjF7NEiyOlQ9N2F-
NALM-6 MULBdI9xfG:|aYOgRZN{[Xl? M2fTPFExKM7:TdMg NUTnS3BPOjRiaB?= M1Toe4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNic3zp[4h1dHl? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ7OUO4N{c,OjF4OUmzPFM9N2F-
JMV-3 Ml31RZBweHSxc3nzJGF{e2G7 M1jzXVExKM7:TdMg NETxToYzPCCq M1XEPYlv\HWlZYOgZ4VtdCCjcH;weI9{cXNic3zp[4h1dHl? M4n4[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkm5N|g{Lz5{MU[5PVM5OzxxYU6=
EHEB MoOySpVv[3Srb36gRZN{[Xl? NF3TSHU2NTVyIN88US=> NFHVc20zPCCq M{PZeIRm[3KnYYPld{BxOjFiZYjwdoV{e2mxbjDzbYdvcW[rY3HueIx6 M{DMe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMU[4N|kyLz5{MUG2PFM6OTxxYU6=
JVM-2  NF2wOJFHfW6ldHnvckBCe3OjeR?= M37welMxKM7:TR?= NFLpUHIzPCCq MkW0[IVkemWjc3XzJJAzOSCneIDy[ZN{cW:w M{XHeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMU[4N|kyLz5{MUG2PFM6OTxxYU6=
KBM3/Bu2506 NH3yR4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|IxRTBwNkegxtVO NGfVcmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEmzN|UxQSd-MkC5N|M2ODl:L3G+
KBM3/Bu2506 NXnQU4JGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX:wMlYh|ryP NFvhSHMzPCCq M3XFfIlv[3KnYYPld{B1cGViY3XscEBnemGldHnvckBqdiCVLYDoZZNm MljMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB7M{O1NFkoRjJyOUOzOVA6RC:jPh?=
MDA-MB-231 MkOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nESWlEPTB;ND6wJO69VQ>? Ml7wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NEezPVAoRjJyNES3N|kxRC:jPh?=
MCF-7 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rObWlEPTB;MUWuNEDPxE1? NV3iZnVHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OFc{QTBpPkKwOFQ4OzlyPD;hQi=>
HLE-B3  NYjqfVZxTnWwY4Tpc44hSXO|YYm= MnvUNlUh|ryP MXS0PEBp NEHzZ5RjdG:la4OgTWZPNc7|4pETbY5lfWOnZDDTWGFVOSCyaH;zdIhwenmuYYTpc44h[W6mIFnEU{BmgHC{ZYPzbY9v NViy[WRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0N|UyPThpPkKwOFM2OTV6PD;hQi=>
K562 NXzMTpJMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPpO|IhcA>? MVLJR|UxRTNwMzDuUS=> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODNyN{G5PEc,OjB|MEexPVg9N2F-
BW-225 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYDJR|IxRTFwM{egx7cyOOLKkklCpO69VcLi M2L5[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Nk[xN|gxLz5zOE[2NVM5ODxxYU6=
OH-65 MmTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnJR|IxRTFwM{egx7cyOOLKkklCpO69VcLi MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ4MUO4NEc,OTh4NkGzPFA9N2F-
GR-145 MnPFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjwTWMzOD1{Lke0JOOYKDFy4pkSPEAh|ryPwrC= NEP1OIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[2NVM5OCd-MUi2OlE{QDB:L3G+
A549 MkfYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnkTWMzOD13LkS4JOOYKDFy4pkSPEDPxE4EoB?= MmX3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NkGzPFAoRjF6Nk[xN|gxRC:jPh?=
CaSki  MkDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XvN2lEOjB;MT6zO{DEnyBzMPMIllch|ryPwrC= Mof6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh4NkGzPFAoRjF6Nk[xN|gxRC:jPh?=
ZMK-1 NYHLOI53T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHLTWMzOD1zLkO3JOOYKDFy4pkSOkDPxE4EoB?= M4jzNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Nk[xN|gxLz5zOE[2NVM5ODxxYU6=
SKW6.4 MlTWRZBweHSxc3nzJGF{e2G7 M4TUWlAvODFvMUCg{txO NGHkZm4zPC92ODDo MV;pcoR2[2W|IHPlcIwh\GWjdHigbY4h[m:2aDD0bY1mNSCjbnSg[I9{\S1iZHXw[Y5l\W62IH3hco5meg>? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB7MkO0NEc,OThyOUKzOFA9N2F-
RPMI 8226 NWjuN5hsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDoNlQhcA>? NYPaTnE1UUN3ME2xMlU1yqEQvF2= M3nDPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUe2NVg3Lz5zN{m3OlE5PjxxYU6=
MM.1S NVT3V29QT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVy0PEBp M1vY[WlEPTB;MUOuOFjDqM7:TR?= NGD4dJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{m3OlE5Pid-MUe5O|YyQDZ:L3G+
MM.1R M{HKfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX2xSWtlPDhiaB?= MWHJR|UxRTN|Lke5JO69VQ>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl5NkG4Okc,OTd7N{[xPFY9N2F-
U937 M{fUcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HjR2lEPTB;MzyyNFAhyrFiNU[wJI5O NEL1ZY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUmzNFM3OSd-MUW5N|A{PjF:L3G+
CLL5 NX71T3d5SW62aYT1cY9zKGG|c3H5 MoTTOFghcHK| M{PzSWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JGNNVDViY3XscJMhcXOxbHH0[YQh\nKxbTDDUGwheGG2aXXueEBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCjbnHsfZNqeyxiRVO1NEA:KDBwMU[g{txONg>? NXr3bW9YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|M4OzlpPkK0Olc{PzN7PD;hQi=>
K562 Mn;oR5l1d3SxeHnjbZR6KGG|c3H5 NWPVVGlnPzJiaILz NFnW[5hEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBx[WOuaYThfIVtNXKnc3nzeIFvfCCNNU[yJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkK2JO69VS5? NVn4[I1WRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2NFU3PTZpPkKwOlA2PjV4PD;hQi=>
CLL3 NX3FdWhbSW62aYT1cY9zKGG|c3H5 NGi3S4Y1QCCqcoO= MkDRRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgR2xNOyClZXzsd{Bqe2:uYYTl[EBnem:vIFPMUEBx[XSrZX70JIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGGwYXz5d4l{NCCHQ{WwJF0hOC5|NTFOwG0v NXjWcIhjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|M4OzlpPkK0Olc{PzN7PD;hQi=>
CLL4 NYXnXHNKSW62aYT1cY9zKGG|c3H5 NHmwcZE1QCCqcoO= MnzwRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgR2xNPCClZXzsd{Bqe2:uYYTl[EBnem:vIFPMUEBx[XSrZX70JIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGGwYXz5d4l{NCCHQ{WwJF0hOC54NDFOwG0v Mor1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{O3N|koRjJ2NkezO|M6RC:jPh?=
CEM-DNR-B NGTVU|ZEgXSxdH;4bYNqfHliYYPzZZk> NXLIWWIxPzJiaILz MlHzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2VONUSQUj3CJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkCxJO69VS5? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZyNU[1Okc,OjB4MEW2OVY9N2F-
primary CLL MV3DfZRwfG:6aXPpeJkh[XO|YYm= NFjsNVREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBxemmvYYL5JGNNVCClZXzsd{whVER3MDC9JFEvOSEQvF2u MlPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNEizPVIoRjJ3MUS4N|kzRC:jPh?=
CLL6 Mm\BRY51cXS3bX;yJIF{e2G7 NHiwWFk1QCCqcoO= MULBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDDUGw3KGOnbHzzJIl{d2yjdHXkJIZzd21iQ1zMJJBifGmnboSgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IF\BR3Mh[W6jbInzbZMtKEWFNUCgQUAyNjZizszNMi=> NUTkPFNoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|M4OzlpPkK0Olc{PzN7PD;hQi=>
CLL2 NXL6cIZTSW62aYT1cY9zKGG|c3H5 MnzSOFghcHK| MnTORY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgR2xNOiClZXzsd{Bqe2:uYYTl[EBnem:vIFPMUEBx[XSrZX70JIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDGRWNUKGGwYXz5d4l{NCCHQ{WwJF0hOi54NjFOwG0v NULFU2FYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|M4OzlpPkK0Olc{PzN7PD;hQi=>
HCT116 NE\qdVdEgXSxdH;4bYNqfHliYYPzZZk> MYC3NkBpenN? MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3MDC9JFYvPiEQvF2u MkPBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NkKyO|coRjJ3NE[yNlc4RC:jPh?=
PBMC NEDwZ2pEgXSxdH;4bYNqfHliYYPzZZk> M3v0b|Q5KGi{cx?= NVjES4NNS3m2b4TvfIlkcXS7IHHnZYlve3RicHH0bYVvfCCSQl3DJIFnfGW{IES4JIhzeyCkeTDD[YxtXGm2cnWtRox2\SCjc4PhfUBqdiCycnXz[Y5kKG:oIH3veZNmKE1{MUDCOEBk\WyuczygTWM2OCB;IEGwJO69VS5? M3PRSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NU[yOFE4Lz5{NUW2NlQyPzxxYU6=
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LAN-5 NXrTOoY5eUiWUzDhd5NigQ>? MnnLdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| NF;vXI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh18 MkTzdWhVWyCjc4PhfS=> MXLxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? NWfpTZRVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 NYXUcWpYeUiWUzDhd5NigQ>? MmTidWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIyPjR|IHPlcIx{ NInESJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC MWHxTHRUKGG|c3H5 NHHCeXlyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLV3DJINmdGy| NGLiSHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SJ-GBM2 MnrGdWhVWyCjc4PhfS=> NX\R[HFyeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= MlPlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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Saos-2 MYnxTHRUKGG|c3H5 MlHvdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV4Fwey1{IHPlcIx{ MlT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 Mk\TdWhVWyCjc4PhfS=> NXn5PGJXeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiT4L0bI9od26jbDCzSEB3cWGkaXzpeJkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
RD NWGybGpueUiWUzDhd5NigQ>? NEPoeHpyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFKGIHPlcIx{ MoT0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
procaspase-9 / procaspase-3; 

PubMed: 27223263     


Procaspase-9 and procaspase-3 were analyzed by Western blot in CLL cells from three patients after a 48 h-incubation in the absence or presence of 3 μM aphidicolin (APC) with or without fludarabine at 0.3 and 1 μM. β-Actin served as a loading control.

p-p53 / p53; 

PubMed: 27223263     


(A–B) CLL cells from 3 different patients were incubated for 24 h with fludarabine at the indicated concentrations in the absence or presence of 3 μM aphidicolin (APC). Phosphorylation of p53 at Ser-15 and total p53 were analyzed by Western blot. Data sho䲧疝Ỵ疞㧀疜膉痘瘿�෋ᾰƌ෋à㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒

STAT1; 

PubMed: 26677135     


Representative western blot analysis of STAT1 in RAW 264.7 cells treated with STAT1 inhibitor fludarabine (Flu) for 24 h.

27223263 26677135
Immunofluorescence
α-SMA / Vimentin; 

PubMed: 28322315     


FLU (50 μM) reduces the expression of α-SMA and Vimentin protein in primary mouse activated HSCs (Hepatic Stellate Cells).

28322315
Growth inhibition assay
Cell viability ; 

PubMed: 24956101     


CLL cells RPMI/0.1% FBS were cultured on 0.5% BSA or 150 nM MMP-9 for 1 h prior to adding the indicated concentrations of fludarabine (Fluda). After 48 h (Fluda) cell viability was determined by flow cytometry using FITC-Annexin V and PI.

24956101
体内研究 PBS对照组中肿瘤细胞增长迅速,25天内扩增为10倍。而用40mg/kgFludarabine处理的肿瘤扩增不超过5倍。40mg/kgFludarabine对RPMI 8226细胞有明显的抗肿瘤效果。RPMI 8226处理肿瘤细胞十天后增加凋亡细胞核数量。[1] Fludarabine可在免疫缺陷小鼠中有效抑制RPMI 8226细胞的骨髓转移。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: 地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系
  • Concentrations: 2 μg/Ml
  • Incubation Time: 24 小时
  • Method:

    对地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系以5 × 105细胞密度铺板,实验组均包括氟达拉滨处理和空白对照组。处理后细胞用PBS清洗两次然后用70%冰乙醇固定,离心后用含100μg/mL核糖核酸酶PBS重悬浮,37ºC孵育30分钟。样品重悬于25μg/mL碘化吡啶中,用流式细胞仪检测。根据产品说明用Annexin V-FITC试剂盒检测细胞凋亡。利用TUNEL(末端脱氧核苷酸转移酶介导脱氧核苷酸缺口末端标记)法和原位细胞凋亡检测试剂盒处理细胞,最后用流式细胞仪分析。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带RPMI 8226肿瘤细胞的免疫缺陷型小鼠
  • Dosages: 40 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 57 mg/mL (199.83 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
8 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 285.23
化学式

C10H12FN5O4

CAS号 21679-14-1
储存条件 粉状
溶于溶剂
别名 FaraA, Fludarabinum

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03832127 Not yet recruiting Drug: 18F-Fludarabine Myeloma Nantes University Hospital|Cyceron April 1 2022 Phase 1
NCT04526795 Recruiting Drug: Cytarabine|Drug: Fludarabine|Drug: Pegcrisantaspase Blast Phase Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent Acute Biphenotypic Leukemia|Recurrent Acute Lymphoblastic Leukemia|Recurrent Acute Myeloid Leukemia|Recurrent Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent T-Cell Prolymphocytic Leukemia|Refractory Acute Biphenotypic Leukemia|Refractory Acute Lymphoblastic Leukemia|Refractory Acute Myeloid Leukemia|Refractory Chronic Myelogenous Leukemia BCR-ABL1 Positive|Refractory T-Cell Prolymphocytic Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) April 9 2021 Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    how to re-suspend and deliver the inhibitor for in vivo experiments?

  • 回答:

    For S1491, Fludarabine, we tested a vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W that you can resuspend the compound in at up to 30mg/ml. It's a suspension and can only be given via oral gavage.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID