Fludarabine (NSC 118218)

For research use only. Not for use in humans.

目录号:S1491 别名: FaraA, Fludarabinum 中文名称:氟达拉滨

Fludarabine (NSC 118218) Chemical Structure

CAS No. 21679-14-1

Fludarabine (NSC 118218, FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1032.75 现货
RMB 810.59 现货
RMB 2525.07 现货
RMB 3836.33 现货
RMB 7289.1 现货
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客户使用Selleck生产的Fludarabine (NSC 118218)发表文献103篇:

产品安全说明书

STAT抑制剂选择性比较

生物活性

产品描述 Fludarabine (NSC 118218, FaraA, Fludarabinum)在血管平滑肌细胞中是一种STAT1活化抑制剂,特异性降低STAT1蛋白质水平(和mRNA水平) ,而对其他STATs无此作用。它还是一种DNA合成抑制剂。Fludarabine可诱导凋亡。
靶点
STAT1 [4]
(Vascular smooth muscle cells)
体外研究

Fludarabine有效抑制RPMI8226细胞增殖,呈时间浓度依赖性。Fludarabine处理MM.1S和MM.1R细胞48小时的IC50分别为13.48μg/mL和33.79μg/mL。而Fludarabine处理U266细胞48小时的IC 50为222.2μg/mL。Fludarabine处理可增加处于细胞周期G 1期的细胞数量,并伴随着处于细胞周期S期细胞数量的减少,对细胞周期的影响呈时间依赖性。Fludarabine在MM细胞中诱导细胞周期阻滞和细胞凋亡。Fludarabine时间依赖性的诱导caspase,-8,- 9和- 3,- 7的剪切,继而诱导PARP的剪切 。氟达拉滨以时间梯度增加Bax基因表达,而Bak基因表达量不变。RPMI 8226细胞用Fludarabine处理12小时,处理后细胞膜电位降低,61.05%的细胞表达低荧光罗丹明123,而未处理的对照组细胞仅为8.62%[1]。为提高溶解度,Fludarabine可制为一磷酸盐形式(F-ara-AMP, fudarabine),在静脉滴注后即可即时、定量的从母核上去磷酸化。细胞内重磷酸化修饰导致Fludarabine-阿糖胞苷三磷酸((F-ara-ATP)。该代谢产物抑制了DNA复制的几个关键过程,如DNA合成所需酶,比如核糖核酸还原酶,DNA引发酶,DNA聚合酶,具有3 ' - 5 '外切酶活性的脱氧核糖核酸聚合酶δ和ε和DNA连接酶[2]。通过检测ICAM - 1的表达和IL - 8释放,表明Fludarabine还可以诱导前炎症刺激单核细胞的增加[3]。Fludarabine对卵巢癌细胞系生长无影响,而它对黑色素瘤细胞系有明显的剂量依赖性抑制[4]。Fludarabine诱导显著降低STAT - 1磷酸化,但不改变JAK 2活性。磷酸Fludarabine对三种STAT蛋白磷酸化影响不显着。Fludarabine(1.5mg)在处理2天后和7天后显著降低磷酸化STAT - 1和STAT-1总蛋白量[5]。 磷酸化JAK 2在处理后第2天变化不明显,处理后第7天的JAK表达明显受抑制。Fludarabine不影响其他STAT蛋白质,特异性抑制STAT - 1激活以降低血管平滑肌细胞体外增殖。[5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Jeko-1  MljsSpVv[3Srb36gRZN{[Xl? Ml\ENlAh|ryP NWe2Z2JiOjRiaB?= M2PxZ4lvcGmkaYTzJIV5eHKnc4Ppc44hd2ZiSVTP MornQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NEC3NVIoRjJ3OUSwO|EzRC:jPh?=
MV-4-11 MkL0RZBweHSxc3nzJGF{e2G7 NWPBWoNjOi53IN88US=> MojTOFghcA>? NFPnfVZqdmS3Y3XzJIFxd3C2b4Ppd{B{dGmpaITsfS=> NYfLRZY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
THP-1 NXS4N2JISXCxcITvd4l{KEG|c3H5 Mon3Nk42KM7:TR?= NEXibG41QCCq MVjpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= NH;UWHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGxNVU5Oyd-MkWxNVE2QDN:L3G+
MOLM 13 NE\xRmxCeG:ydH;zbZMhSXO|YYm= M335eVIvPSEQvF2= NHLONpA1QCCq M4HWN4lv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 NUXMOmFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVE2QDNpPkK1NVEyPTh|PD;hQi=>
KBM3/Bu2506 MUTBdI9xfG:|aYOgRZN{[Xl? NULl[IdbOi53IN88US=> M3rsflQ5KGh? NWWy[XIxcW6mdXPld{BieG:ydH;zbZMhe2yrZ3j0cJk> MmP1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzMUG1PFMoRjJ3MUGxOVg{RC:jPh?=
Nalm-6 M1SwOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWnJR|UxRTF6IN88US=> MnKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Reh MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVfJR|UxRTNyIN88US=> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
U2937 MkX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfTcXF7UUN3ME2xOkDPxE1? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
Mec-1 MlHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXqTWM2OO,:nkWwNEDPxE1? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTB4MUGwNUc,OjVyNkGxNFE9N2F-
RPMI-8226 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGmzfo1KSzVyPUWwNEDPxE1? NXPjO2U5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWwOlEyODFpPkK1NFYyOTBzPD;hQi=>
Molt-4 NFv4XZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWXJR|UxRTF6MDFOwG0> NGr5eHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUC2NVExOSd-MkWwOlEyODF:L3G+
Nalm-6-FluR MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLFb3AxUUN3ME2yOVAh|ryP Mne2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyNkGxNFEoRjJ3ME[xNVAyRC:jPh?=
Raji  MWHGeY5kfGmxbjDBd5NigQ>? M1vrclPDqM7:TR?= MYeyOE81QC95MjDo NXvUVYY1cW6mdXPld{Bi[2O3bYXsZZRqd26|IH;mJJA2OyxicE[zJIFv\CCyN{RCpC=> M{Pa[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUSwOlk2Lz5{NEm0NFY6PTxxYU6=
PBMC MWHGeY5kfGmxbjDBd5NigQ>? M2HDUVUxNzFyMDFOwG0> M3LwW|I1KGh? NGLCNFFFVVOR NW\xe4tXcW6qaXLpeJMhW1SDVEGgdIhwe3Cqb4L5cIF1cW:w MlrRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MUG4O|IoRjJ2OUGxPFczRC:jPh?=
MDA-231 NInBRWdHfW6ldHnvckBCe3OjeR?= M4rKZ|ExOCEQvF2= MmnUNlQhcA>? M1PZdGROW09? Mn7k[IVkemWjc3XzJGlFVyCneIDy[ZN{cW:w M{GxU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUGxPFczLz5{NEmxNVg4OjxxYU6=
624.38mel  NGftTmtHfW6ldHnvckBCe3OjeR?= MkS3OVAh|ryP MmjSNlQhcA>? MlXUSG1UVw>? MX7k[YNz\WG|ZYOgTWRQKGW6cILld5Nqd25? NXrXSoM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
MDA-231 MnPQSpVv[3Srb36gRZN{[Xl? NVfMcmNtPTBvMkCwJO69VQ>? MmD5NlQhcA>? M{XBSmROW09? NYHOOFVTcW6qaXLpeJMhUUSRIHHjeIl3cXS7IHnu[IVx\W6mZX70cJkhd2ZibWLORUBt\X[nbIO= NGLofXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmxNVg4Oid-MkS5NVE5PzJ:L3G+
624.38mel  M{DEOGZ2dmO2aX;uJGF{e2G7 NWjaZpVpPTBvMkCwJO69VQ>? NFPSW|czPCCq MmD0SG1UVw>? NIHSTYlqdmirYnn0d{BKTE9iYXP0bZZqfHliaX7k[ZBmdmSnboTsfUBw\iCvUl7BJIxmfmWucx?= NVWzUJFDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NVE5PzJpPkK0PVEyQDd{PD;hQi=>
HMECs MlL6SpVv[3Srb36gRZN{[Xl? MUCxNFDDqM7:TdMg NVHibIFMOzcEoHi= NX7WWYp{cW6qaXLpeJMhUU[QzsRCpIFv\CCOUGOgbY5lfWOnZDDTWGFVOSCyaH;zdIhwenmuYYTpc44h[W6mIFnSSlEh\XiycnXzd4lwdg>? NH3ab2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxNVMzPyd-MkSyNVE{Ojd:L3G+
HMECs  Mlv0SpVv[3Srb36gRZN{[Xl? NHrqNGgyODEEoN88UeKh MU[zOuKhcA>? Mli5bY5pcWKrdIOgTWZP|rIEoH3l[IlifGWmIIDoc5NxcG:{eXzheIlwdiCxZjDTWGFVOSCjbnSgV3RCXDNuIHL1eEBvd3Rib3[gV3RCXDJ? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJzMUOyO{c,OjR{MUGzNlc9N2F-
BJAB M3fuZWFxd3C2b4Ppd{BCe3OjeR?= MlrCOeKh|ryP MUGyOEBp NXW5SodocW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB3N{G0O{c,OjRyNUexOFc9N2F-
I-83 M3TvPWFxd3C2b4Ppd{BCe3OjeR?= M4XNR|XDqM7:TR?= MkfxNlQhcA>? MYHpcoR2[2W|IHPlcIwh[XCxcITvd4l{ NVHrcVBlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOVcyPDdpPkK0NFU4OTR5PD;hQi=>
NALM6 NXHwVHhvSXCxcITvd4l{KEG|c3H5 NXH0V5lGPcLizszN M3\sdlI1KGh? NYmzTZNRcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= NW\HTY9ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwOVcyPDdpPkK0NFU4OTR5PD;hQi=>
DU-145 Ml[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIPXSWUxNTFyIN88[{9udA>? MYW0PEBpyqB? M3:2TIlvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NWHRSnkyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3N|Q5OTVpPkKzO|M1QDF3PD;hQi=>
Nalm-6 NGP4SFVHfW6ldHnvckBCe3OjeR?= NEf2epkyOMLizszN NEDKfYkyNzJxNDDo M4DtPIlv\HWlZYOgZZV1d3CqYXf5 NIWz[5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[4NVIzOyd-MkO2PFEzOjN:L3G+
Reh NVf1Sm0zTnWwY4Tpc44hSXO|YYm= MnfTNVDDqM7:TR?= NHfEcXoyNzJxNDDo MoTxbY5lfWOnczDheZRweGijZ4m= MnzaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEGyNlMoRjJ|NkixNlI{RC:jPh?=
Nalm-6 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrmVGxyUUN3MDFijNwyOOLCid88US=> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ6MUKyN{c,OjN4OEGyNlM9N2F-
Reh M1vYbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;jeo5KSzVyIPMIwFEx6oDLzszN Ml3BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4OEGyNlMoRjJ|NkixNlI{RC:jPh?=
HEC1A NFu0WHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\wNVAxNTVyMDFOwG0> NF\xNGczPCCq NYTuRo54cW6qaXLpeJMh[2WubDDndo94fGhiaX6gZUBld3OnLXTldIVv\GWwdDDtZY5v\XJ? M2PFXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
AN3CA MkmwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\CVGMyODBvNUCwJO69VQ>? NWn4fHdWOjRiaB?= M{DD[olvcGmkaYTzJINmdGxiZ4Lve5RpKGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NWHFdXUyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1PVU3QTdpPkKzOVk2Pjl5PD;hQi=>
HEC50B M17BSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1v2OVExOC13MECg{txO NGS5[YUzPCCq MYXpcohq[mm2czDj[YxtKGe{b4f0bEB{dGmpaITsfS=> M13UZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUm1Olk4Lz5{M{W5OVY6PzxxYU6=
HEC1A NGn4XmFCeG:ydH;zbZMhSXO|YYm= MVmyNE8yODBizszN NGW0VHAzPCCq MljYbY5lfWOnczDhdI9xfG:|aYOgbY4h[SCmb4PlMYRmeGWwZHXueEBu[W6wZYK= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
AN3CA Ml3rRZBweHSxc3nzJGF{e2G7 M3f2fFIxNzFyMDFOwG0> NXrIVZpHOjRiaB?= MUHpcoR2[2W|IHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NEH0Opo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W5OVY6Pyd-MkO1PVU3QTd:L3G+
HEC50B MXHBdI9xfG:|aYOgRZN{[Xl? NELVeIIzOC9zMECg{txO NHL0TG0zPCCq MYnpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV7NU[5O{c,OjN3OUW2PVc9N2F-
EHEB MVTBdI9xfG:|aYOgRZN{[Xl? MVO0NEDPxE1? NH;O[ZQzPCCq M4DXcYlv\HWlZYOgZZBweHSxc3nz NF;vNYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5O|A4PSd-MkO0PVcxPzV:L3G+
A549 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGn5XlRKSzVyPUG1MlfDuTJwODFCuW0> NIqwWHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O3O|E6Oid-MkOzO|cyQTJ:L3G+
A549 GAPDH-deficient M3;6NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PjZ2lEPTB;MUiuOeKyOi5|INM1US=> M3XiTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{e3NVkzLz5{M{O3O|E6OjxxYU6=
CLL  Mm[xRZBweHSxc3nzJGF{e2G7 MoL4NVAh|ryPwrC= MUGyOE06PiCq MkLybY5lfWOnczDhdI9xfG:2aXOgZ4VtdCCmZXH0bC=> NUGxW|VYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyNFc3QDZpPkKyNlA4Pjh4PD;hQi=>
MEC1 M3nRTmFxd3C2b4Ppd{BCe3OjeR?= NITncmQyODEEoN88US=> Mkm0O|IhcA>? MXHpcoR2[2W|IHHwc5B1d3OrczDzbYdvcW[rY3HueIx6 MmjjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzM{K5O|MoRjJ{MUOyPVc{RC:jPh?=
U937  MX3BdI9xfG:|aYOgRZN{[Xl? M3vURVAvQCEQvF2= M3jkWlQuPDhiaB?= MV3pcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= MoC5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyN{S3NFAoRjJ{MEe0O|AxRC:jPh?=
U937  NGP1NXRCeG:ydH;zbZMhSXO|YYm= NYTkXYRPOSEQvF2= NVjRN3pvQTZiaB?= MojEbY5lfWOnczDhdI9xfG:|aYOgd4xq\2i2bIm= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB{M{WyN{c,OjJyMkO1NlM9N2F-
Daudi M1v5d2Fxd3C2b4Ppd{BCe3OjeR?= NVX3cXZSOjBizszN NGfpN4w6PiCq M{jXWIlv\HWlZYOgZZBweHSxc3nzJJNtcWeqdHz5 NGLleHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCyN|UzOyd-MkKwNlM2OjN:L3G+
J45.01 MXHBdI9xfG:|aYOgRZN{[Xl? MUmxJO69VQ>? Ml3iPVYhcA>? MYDpcoR2[2W|IHHwc5B1d3OrczDzcIlocHSueR?= NFXLUog9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCyN|UzOyd-MkKwNlM2OjN:L3G+
RPMI 8226 M3jqRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDw[XdHUUN3ME2yOU46yqEEsdMgN{44KM7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OEK2OEc,OjF7NEiyOlQ9N2F-
CEM NYHnc4YyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4XnSmlEPTB;Mj60xsDDucLiMD60JO69VQ>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OEK2OEc,OjF7NEiyOlQ9N2F-
Raji NETLfWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vTdGlEPTB;MD60O:KhyrIEoECuNFQh|ryP NX23PW84RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5OFgzPjRpPkKxPVQ5OjZ2PD;hQi=>
U937 NYLsZZlwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NICxWYJKSzVyPUCuNlTDqMLzwrCwMlA1KM7:TR?= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OEK2OEc,OjF7NEiyOlQ9N2F-
K562 NUTjd4h{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mln6TWM2OD1yLkS0xsDDucLiMD6wOUDPxE1? M1LkVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUS4NlY1Lz5{MUm0PFI3PDxxYU6=
NALM-6 NFzMd|JCeG:ydH;zbZMhSXO|YYm= NHHkXnAyOCEQvF5CpC=> MUiyOEBp NXfXWVNTcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzcIlocHSueR?= M4HOZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkm5N|g{Lz5{MU[5PVM5OzxxYU6=
JMV-3 MkSwRZBweHSxc3nzJGF{e2G7 NWXTfZd[OTBizszNxsA> MXWyOEBp NWPOdphncW6mdXPld{Bk\WyuIHHwc5B1d3OrczDzcIlocHSueR?= M2fnZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkm5N|g{Lz5{MU[5PVM5OzxxYU6=
EHEB MWPGeY5kfGmxbjDBd5NigQ>? MoT4OU02OCEQvF2= NGrIVW4zPCCq MnHw[IVkemWjc3XzJJAzOSCneIDy[ZN{cW:wIIPp[45q\mmlYX70cJk> M3r2OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMU[4N|kyLz5{MUG2PFM6OTxxYU6=
JVM-2  NH7vfGVHfW6ldHnvckBCe3OjeR?= NFX2RmY{OCEQvF2= M3HtVFI1KGh? Mojy[IVkemWjc3XzJJAzOSCneIDy[ZN{cW:w MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF4OEO5NUc,OjFzNkizPVE9N2F-
KBM3/Bu2506 M13nWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGjYVmZKSzJyPUCuOlchyrWP NIXEOVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEmzN|UxQSd-MkC5N|M2ODl:L3G+
KBM3/Bu2506 MnvxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlvxNE43KM7:TR?= NWnkZo92OjRiaB?= NHvwXVhqdmO{ZXHz[ZMhfGinIHPlcIwh\nKjY4Tpc44hcW5iUz3wbIF{\Q>? MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODl|M{WwPUc,OjB7M{O1NFk9N2F-
MDA-MB-231 MoTxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHqfVNWUUN3ME20MlAh|ryP MnyzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NEezPVAoRjJyNES3N|kxRC:jPh?=
MCF-7 M4SxNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTF3LkCg{txO Ml\TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NEezPVAoRjJyNES3N|kxRC:jPh?=
HLE-B3  NV76[4ZxTnWwY4Tpc44hSXO|YYm= M4\xPFI2KM7:TR?= NF;KPZM1QCCq MUPicI9kc3NiSV\OMe6{6oDVaX7keYNm\CCVVFHUNUBxcG:|cHjvdplt[XSrb36gZY5lKEmGTzDlfJBz\XO|aX;u M1vMeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEO1NVU5Lz5{MESzOVE2QDxxYU6=
K562 M1LVNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTv[WFyPzJiaB?= NF7YfFVKSzVyPUOuN{BvVQ>? NUn0[5ZxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzNFcyQThpPkKwN|A4OTl6PD;hQi=>
BW-225 M1jqbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF;hZW1KSzJyPUGuN|chy5dzMPMIlljDqM7:TdMg NHfOVZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[2NVM5OCd-MUi2OlE{QDB:L3G+
OH-65 NWHtcmJET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2O3TmlEOjB;MT6zO{DEnzFy4pkSPOKh|ryPwrC= NVrkSXdzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi2OlE{QDBpPkG4OlYyOzhyPD;hQi=>
GR-145 M2PGRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|IxRTJwN{Sgx7chOTEkiKK4JEDPxE4EoB?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ4MUO4NEc,OTh4NkGzPFA9N2F-
A549 NHfrW4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HYW2lEOjB;NT60PEDEnyBzMPMIllgh|ryPwrC= M4qwWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Nk[xN|gxLz5zOE[2NVM5ODxxYU6=
CaSki  MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGXJeHlKSzJyPUGuN|chy5diMUFijLI4KM7:TdMg MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDZ4MUO4NEc,OTh4NkGzPFA9N2F-
ZMK-1 NUjKWZpFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrST|hKSzJyPUGuN|chy5diMUFijLI3KM7:TdMg M{HIZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Nk[xN|gxLz5zOE[2NVM5ODxxYU6=
SKW6.4 NFvoPI1CeG:ydH;zbZMhSXO|YYm= NGDCPGUxNjBzLUGwJO69VQ>? M{XKTVI1NzR6IHi= NVvnTFVPcW6mdXPld{Bk\WyuIHTlZZRpKGmwIHLveIghfGmvZT2gZY5lKGSxc3WtJIRmeGWwZHXueEBu[W6wZYK= NUXnSIhkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiwPVI{PDBpPkG4NFkzOzRyPD;hQi=>
RPMI 8226 Ml3TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkfSNlQhcA>? MmXuTWM2OD1zLkW0xsDPxE1? Mk\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7N{[xPFYoRjF5OUe2NVg3RC:jPh?=
MM.1S NWjyUVNRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVewcXdLPDhiaB?= Mm\QTWM2OD1zMz60POKh|ryP MmHkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7N{[xPFYoRjF5OUe2NVg3RC:jPh?=
MM.1R NXrTWVNUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3zPb|Q5KGh? MmfPTWM2OD1|Mz63PUDPxE1? Ml\OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7N{[xPFYoRjF5OUe2NVg3RC:jPh?=
U937 M{XySGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDmcmlGUUN3ME2zMFIxOCEEsTC1OlAhdk1? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTl|MEO2NUc,OTV7M{CzOlE9N2F-
CLL5 MlexRY51cXS3bX;yJIF{e2G7 MUK0PEBpenN? MYDBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDDUGw2KGOnbHzzJIl{d2yjdHXkJIZzd21iQ1zMJJBifGmnboSgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IF\BR3Mh[W6jbInzbZMtKEWFNUCgQUAxNjF4IN88UU4> M{nB[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkezO|M6Lz5{NE[3N|c{QTxxYU6=
K562 MUTDfZRwfG:6aXPpeJkh[XO|YYm= NVW5WIRuPzJiaILz MkDuR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gdIFkdGm2YYjlcE1z\XOrc4ThcpQhUzV4MjDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5{NjFOwG0v MnezQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4MEW2OVYoRjJyNkC1OlU3RC:jPh?=
CLL3 NXPSVXBySW62aYT1cY9zKGG|c3H5 NEHl[pk1QCCqcoO= M32ybmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JGNNVDNiY3XscJMhcXOxbHH0[YQh\nKxbTDDUGwheGG2aXXueEBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCjbnHsfZNqeyxiRVO1NEA:KDBwM{Wg{txONg>? NF\PVGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[3N|c{QSd-MkS2O|M4Ozl:L3G+
CLL4 NHLO[2hCdnSrdIXtc5Ih[XO|YYm= MVy0PEBpenN? NVrQN2FNSW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhS0yONDDj[YxteyCrc3;sZZRm\CCocn;tJGNNVCCyYYTp[Y51KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBHSUOVIHHuZYx6e2m|LDDFR|UxKD1iMD62OEDPxE1w M1HzSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkezO|M6Lz5{NE[3N|c{QTxxYU6=
CEM-DNR-B MWXDfZRwfG:6aXPpeJkh[XO|YYm= M4nsWFczKGi{cx?= NFW5UmJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBETU1vRF7SMWIh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGuNFEh|ryPLh?= MneyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4MEW2OVYoRjJyNkC1OlU3RC:jPh?=
primary CLL NFvMUJREgXSxdH;4bYNqfHliYYPzZZk> NYnvTG06S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4heHKrbXHyfUBEVExiY3XscJMtKEyGNUCgQUAyNjFizszNMi=> NFHHR|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG0PFM6Oid-MkWxOFg{QTJ:L3G+
CLL6 MVTBcpRqfHWvb4KgZZN{[Xl? MVO0PEBpenN? NVj1dVlESW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhS0yONjDj[YxteyCrc3;sZZRm\CCocn;tJGNNVCCyYYTp[Y51KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBHSUOVIHHuZYx6e2m|LDDFR|UxKD1iMT62JO69VS5? MnXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4N{O3N|koRjJ2NkezO|M6RC:jPh?=
CLL2 MoX6RY51cXS3bX;yJIF{e2G7 MoewOFghcHK| M{DXOGFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JGNNVDJiY3XscJMhcXOxbHH0[YQh\nKxbTDDUGwheGG2aXXueEBie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgSmFEWyCjbnHsfZNqeyxiRVO1NEA:KDJwNk[g{txONg>? M3HrcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NkezO|M6Lz5{NE[3N|c{QTxxYU6=
HCT116 M2nGSWN6fG:2b4jpZ4l1gSCjc4PhfS=> M1jyVFczKGi{cx?= NGjoRW9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KFOUQjDhd5NigSxiSVO1NEA:KDZwNjFOwG0v NWfnW|VYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlIzPzdpPkK1OFYzOjd5PD;hQi=>
PBMC NInRT3lEgXSxdH;4bYNqfHliYYPzZZk> M1fN[FQ5KGi{cx?= Ml3GR5l1d3SxeHnjbZR6KGGpYXnud5QheGG2aXXueEBRSk2FIHHmeIVzKDR6IHjyd{BjgSCFZXzsWIl1emVvQnz1[UBie3OjeTDpckBxemW|ZX7jJI9nKG2xdYPlJG0zOTCENDDj[YxteyxiSVO1NEA:KDFyIN88UU4> MnXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3NkK0NVcoRjJ3NU[yOFE4RC:jPh?=
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SK-N-MC Mn;DdWhVWyCjc4PhfS=> MWDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= NWG3Vo9uRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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Rh18 M4\zVpFJXFNiYYPzZZk> M2jYfJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy| M4C2[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 M131ZpFJXFNiYYPzZZk> NHrjPY9yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBPSjF4NEOgZ4VtdHN? NH61Z2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC NVnSWotZeUiWUzDhd5NigQ>? MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNU2FIHPlcIx{ M2HyR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 NIXPNotyUFSVIHHzd4F6 MnfBdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgV2ouT0KPMjDj[Yxtew>? NFizSWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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Rh18 MkDZdWhVWyCjc4PhfS=> MUXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSbFE5KGOnbHzz M{OycFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 MV\xTHRUKGG|c3H5 MUDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTZY9{NTJiY3XscJM> MlXSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 NVfm[G57eUiWUzDhd5NigQ>? M1nO[JFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KE:{dHjv[49v[WxiM1Sgeoli[mmuaYT5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= NYTkeVh[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD NWnSVG1LeUiWUzDhd5NigQ>? NEfSVoxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCRcoToc4dwdmGuIEPEJJZq[WKrbHn0fUB{[3KnZX6g[o9zKFKGIHPlcIx{ NYP2dnJ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
procaspase-9 / procaspase-3; 

PubMed: 27223263     


Procaspase-9 and procaspase-3 were analyzed by Western blot in CLL cells from three patients after a 48 h-incubation in the absence or presence of 3 μM aphidicolin (APC) with or without fludarabine at 0.3 and 1 μM. β-Actin served as a loading control.

p-p53 / p53; 

PubMed: 27223263     


(A–B) CLL cells from 3 different patients were incubated for 24 h with fludarabine at the indicated concentrations in the absence or presence of 3 μM aphidicolin (APC). Phosphorylation of p53 at Ser-15 and total p53 were analyzed by Western blot. Data sho䲧疝Ỵ疞㧀疜膉痘 瘿�෋ᾰƌ෋à 㺣痖帉痖Ѐ瑖堘𢡄빢᎒෋à鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒

STAT1; 

PubMed: 26677135     


Representative western blot analysis of STAT1 in RAW 264.7 cells treated with STAT1 inhibitor fludarabine (Flu) for 24 h.

27223263 26677135
Immunofluorescence
α-SMA / Vimentin; 

PubMed: 28322315     


FLU (50 μM) reduces the expression of α-SMA and Vimentin protein in primary mouse activated HSCs (Hepatic Stellate Cells).

28322315
Growth inhibition assay
Cell viability ; 

PubMed: 24956101     


CLL cells RPMI/0.1% FBS were cultured on 0.5% BSA or 150 nM MMP-9 for 1 h prior to adding the indicated concentrations of fludarabine (Fluda). After 48 h (Fluda) cell viability was determined by flow cytometry using FITC-Annexin V and PI.

24956101
体内研究 PBS对照组中肿瘤细胞增长迅速,25天内扩增为10倍。而用40mg/kgFludarabine处理的肿瘤扩增不超过5倍。40mg/kgFludarabine对RPMI 8226细胞有明显的抗肿瘤效果。RPMI 8226处理肿瘤细胞十天后增加凋亡细胞核数量。[1] Fludarabine可在免疫缺陷小鼠中有效抑制RPMI 8226细胞的骨髓转移。

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[1]

- 合并
  • Cell lines: 地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系
  • Concentrations: 2 μg/Ml
  • Incubation Time: 24 小时
  • Method:

    对地塞米松敏感(MM.1S) 和耐受 (MM.1R) 的人MM 细胞系, RPMI8226和 U266 细胞系以5 × 105细胞密度铺板,实验组均包括氟达拉滨处理和空白对照组。处理后细胞用PBS清洗两次然后用70%冰乙醇固定,离心后用含100μg/mL核糖核酸酶PBS重悬浮,37ºC孵育30分钟。样品重悬于25μg/mL碘化吡啶中,用流式细胞仪检测。根据产品说明用Annexin V-FITC试剂盒检测细胞凋亡。利用TUNEL(末端脱氧核苷酸转移酶介导脱氧核苷酸缺口末端标记)法和原位细胞凋亡检测试剂盒处理细胞,最后用流式细胞仪分析。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 携带RPMI 8226肿瘤细胞的免疫缺陷型小鼠
  • Dosages: 40 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 57 mg/mL (199.83 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
8 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 285.23
化学式

C10H12FN5O4

CAS号 21679-14-1
储存条件 粉状
溶于溶剂
别名 FaraA, Fludarabinum

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04526795 Not yet recruiting Drug: Cytarabine|Drug: Fludarabine|Drug: Pegcrisantaspase Blast Phase Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent Acute Biphenotypic Leukemia|Recurrent Acute Lymphoblastic Leukemia|Recurrent Acute Myeloid Leukemia|Recurrent Chronic Myelogenous Leukemia BCR-ABL1 Positive|Recurrent T-Cell Prolymphocytic Leukemia|Refractory Acute Biphenotypic Leukemia|Refractory Acute Lymphoblastic Leukemia|Refractory Acute Myeloid Leukemia|Refractory Chronic Myelogenous Leukemia BCR-ABL1 Positive|Refractory T-Cell Prolymphocytic Leukemia M.D. Anderson Cancer Center|National Cancer Institute (NCI) December 31 2020 Phase 1
NCT03832127 Not yet recruiting Drug: 18F-Fludarabine Myeloma Nantes University Hospital|Cyceron April 1 2019 Phase 1
NCT03696537 Active not recruiting Drug: Fludarabine|Radiation: Total Marrow Irradiation (TMI) Acute Myeloid Leukemia|Chronic Myeloid Leukemia|Acute Lymphocytic Leukemia|Myelodysplastic Syndromes Naoyuki G. Saito M.D. Ph.D.|Indiana University August 29 2018 Phase 1
NCT02718755 Withdrawn Drug: Fludarabine|Drug: Cytarabine|Drug: Erwinase Hematologic Malignancy M.D. Anderson Cancer Center|Jazz Pharmaceuticals May 2018 Phase 2

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    how to re-suspend and deliver the inhibitor for in vivo experiments?

  • 回答:

    For S1491, Fludarabine, we tested a vehicle: 30% Propylene glycol, 5% Tween 80, 65% D5W that you can resuspend the compound in at up to 30mg/ml. It's a suspension and can only be given via oral gavage.

STAT Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID