ZM 447439

For research use only. Not for use in humans.

目录号:S1103

ZM 447439 Chemical Structure

CAS No. 331771-20-1

ZM 447439是一种选择性的,ATP竞争性Aurora AAurora B抑制剂,IC50分别为110 nM和130 nM,作用于Aurora A/B比作用于MEK1, Src, Lck选择性高8倍,对CDK1/2/4, Plk1, Chk1等几乎没有作用效果。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 804.03 现货
RMB 566.63 现货
RMB 899.05 现货
RMB 2227.71 现货
RMB 3857.13 现货
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客户使用Selleck生产的ZM 447439发表文献50篇:

产品安全说明书

Aurora Kinase抑制剂选择性比较

生物活性

产品描述 ZM 447439是一种选择性的,ATP竞争性Aurora AAurora B抑制剂,IC50分别为110 nM和130 nM,作用于Aurora A/B比作用于MEK1, Src, Lck选择性高8倍,对CDK1/2/4, Plk1, Chk1等几乎没有作用效果。
特性 ZM-447439是Aurora选择性ATP竞争性抑制剂
靶点
Aurora A [1]
(Cell-free assay)
Aurora B [1]
(Cell-free assay)
LCK [1]
(Cell-free assay)
Src [1]
(Cell-free assay)
MEK1 [1]
(Cell-free assay)
110 nM 130 nM 880 nM 1.03 μM 1.79 μM
体外研究

体外, ZM-447439 选择性抑制重组人类Aurora A和B,IC50分别为110和130 nM,而抑制其他不同结构类型蛋白激酶,包括有丝分裂激酶CDK1和PLK1时,IC50>10 μM。 [1] Aurora 激酶抑制剂, ZM-447439 抑制BON, QGP-1和MIP-101三种细胞系生长,连续处理72小时,IC50分别为3 μM , 0.9 μM 和3 μM,这种作用存在时间和剂量依赖性。此外, ZM-447439通过促进DNA分裂和 caspase 3 和7激活,有效诱导细胞凋亡,且使GEP-NET细胞在G0/G1期和G2/M期停顿。[2] 在小鼠胚胎中, ZM-447439 抑制Aurora 激酶,通过在每个胚胎周期使用不同干扰,调节G2到中期的组蛋白H3 serine 10 (H3S10Ph)而使前两次分裂发生异常。[3] 最新研究显示ZM-447439作用于宫颈癌SiHa细胞,抑制生长和凋亡,且增强对Cisplatin的化学敏感性。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human EoL-1-cell M3TlWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXTQPIZ7UW6qaXLpeIlwdiCxZjDoeY1idiCHb1ytNU1k\WyuIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MD6xPFY4QCEQvF2= MnLLV2FPT0WU
MCF7 cell M1XqSXBzd2yrZnXyZZRqd25iYYPzZZk> NHrXboJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JG1ETjdiY3XscEBtcW6nLDDJR|UxRTBwMUm4JO69VQ>? MXKxOlM{PzF{Mh?=
human P12-ICHIKAWA cell MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MWXJcohq[mm2aX;uJI9nKGi3bXHuJHAyOi2LQ1jJT2FYSSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwMkK0OFEh|ryP Mon4V2FPT0WU
human KARPAS-45 cell MnzBS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3r1OGlvcGmkaYTpc44hd2ZiaIXtZY4hU0GUUFHTMVQ2KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OC5|OEGyPEDPxE1? NYXSepo4W0GQR1XS
human ES3 cell NXTXc25[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Ml3jTY5pcWKrdHnvckBw\iCqdX3hckBGWzNiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkS3N|Ih|ryP NXX2WYY5W0GQR1XS
human ES8 cell M3Hre2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnLsTY5pcWKrdHnvckBw\iCqdX3hckBGWzhiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1yLkS5PFA3KM7:TR?= NFPBOGxUSU6JRWK=
human TE-11 cell MVXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NEnTSYpKdmirYnn0bY9vKG:oIHj1cYFvKFSHLUGxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE42OzdyMzFOwG0> MVrTRW5ITVJ?
human RS4-11 cell M4[2emdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWTJcohq[mm2aX;uJI9nKGi3bXHuJHJUPC1zMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuOVU1PCEQvF2= NVHYVnVkW0GQR1XS
human MOLT-16 cell NHLyUZFIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1r0WWlvcGmkaYTpc44hd2ZiaIXtZY4hVU:OVD2xOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvPjB7NkGg{txO MoryV2FPT0WU
human RKO cell M4f3Wmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUfJcohq[mm2aX;uJI9nKGi3bXHuJHJMVyClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTBwN{C2OVYh|ryP NIjLZZhUSU6JRWK=
human MV-4-11 cell Mn:3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHywXWNKdmirYnn0bY9vKG:oIHj1cYFvKE2YLUStNVEh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjd7NkOg{txO M2rMUHNCVkeHUh?=
human SW954 cell MnPWS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3XuSmlvcGmkaYTpc44hd2ZiaIXtZY4hW1d7NUSgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlg{PjN3IN88US=> MWDTRW5ITVJ?
human BE-13 cell NG\DVFlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWfWSHJXUW6qaXLpeIlwdiCxZjDoeY1idiCERT2xN{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVAvQDR2MUig{txO M1Py[HNCVkeHUh?=
human MOLT-4 cell MU\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NWXhW2dqUW6qaXLpeIlwdiCxZjDoeY1idiCPT1zUMVQh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0xNjh7OUe4JO69VQ>? NYjUPZR1W0GQR1XS
human NBsusSR cell NWTITYd{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MoXOTY5pcWKrdHnvckBw\iCqdX3hckBPSnO3c2PSJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NE46OTN6NzFOwG0> M320eXNCVkeHUh?=
human H9 cell M1;Ld2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NXfIO|BqUW6qaXLpeIlwdiCxZjDoeY1idiCKOTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUCuPVI6PzlizszN NUm0TXk4W0GQR1XS
human A172 cell NUi0Z4VDT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NGfwSGdKdmirYnn0bY9vKG:oIHj1cYFvKEFzN{KgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2wMlk5PDFzIN88US=> MXPTRW5ITVJ?
human ES5 cell MnX5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NE\XWXlKdmirYnn0bY9vKG:oIHj1cYFvKEWVNTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuNFAzPDhizszN NYrCfXA{W0GQR1XS
human SBC-1 cell M{ToO2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHO4SVRKdmirYnn0bY9vKG:oIHj1cYFvKFOEQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4xOzl{ODFOwG0> NEnuVFRUSU6JRWK=
human NCI-H209 cell NHTO[ohIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYTleVJCUW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFIxQSClZXzsJIdzd3e2aDDpckBiKGOnbHygeoli[mmuaYT5JIF{e2G7LDDJR|UxRTFwMU[2NFIh|ryP M1;2eXNCVkeHUh?=
human NKM-1 cell NIDK[4hIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M37CRmlvcGmkaYTpc44hd2ZiaIXtZY4hVkuPLUGgZ4VtdCCpcn;3eIghcW5iYTDj[YxtKH[rYXLpcIl1gSCjc4PhfUwhUUN3ME2xMlE3Pzl6IN88US=> NXjFVIhVW0GQR1XS
human NCI-H720 cell M1r5OWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFnVWldKdmirYnn0bY9vKG:oIHj1cYFvKE6FST3IO|IxKGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS5{ME[yO{DPxE1? Ml;5V2FPT0WU
human KE-37 cell NUDlZplMT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWH0Nm9sUW6qaXLpeIlwdiCxZjDoeY1idiCNRT2zO{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOjF|OEig{txO M2DBW3NCVkeHUh?=
human SW48 cell MlTGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NWPhVms3UW6qaXLpeIlwdiCxZjDoeY1idiCVV{S4JINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4zOzF3NTFOwG0> M33ZVHNCVkeHUh?=
human IST-SL1 cell MUTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmjRTY5pcWKrdHnvckBw\iCqdX3hckBKW1RvU1yxJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU4{OTd{NzFOwG0> MX;TRW5ITVJ?
human SK-NEP-1 cell M1PQTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnLzTY5pcWKrdHnvckBw\iCqdX3hckBUUy2QRWCtNUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvOzZ2OUig{txO M33QdnNCVkeHUh?=
human NOMO-1 cell Ml3OS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MWDJcohq[mm2aX;uJI9nKGi3bXHuJG5QVU9vMTDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuN|Y4OjVizszN MWnTRW5ITVJ?
human DOHH-2 cell NIjHVo9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYmyT2ZqUW6qaXLpeIlwdiCxZjDoeY1idiCGT1jIMVIh[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0yNjRyMke2JO69VQ>? NHTDflRUSU6JRWK=
human ABC-1 cell MVTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NFrBWGJKdmirYnn0bY9vKG:oIHj1cYFvKEGEQz2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU41ODN3MjFOwG0> MmDpV2FPT0WU
human Ramos-2G6-4C10 cell NH;xbnZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFPIbGhKdmirYnn0bY9vKG:oIHj1cYFvKFKjbX;zMVJIPi12Q{GwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU41ODZyNTFOwG0> NF;Odm9USU6JRWK=
human EM-2 cell NX;LOXg4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYrJcohq[mm2aX;uJI9nKGi3bXHuJGVONTJiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLkSxO|IyKM7:TR?= NGL4TGxUSU6JRWK=
human NB14 cell MXjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{fufGlvcGmkaYTpc44hd2ZiaIXtZY4hVkJzNDDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUGuOVU3OjFizszN NInBSZdUSU6JRWK=
human MOLT-13 cell MlXVS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlSxTY5pcWKrdHnvckBw\iCqdX3hckBOV0yWLUGzJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU42PjdyNjFOwG0> Mkn2V2FPT0WU
human ECC10 cell MlTGS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYDJcohq[mm2aX;uJI9nKGi3bXHuJGVESzFyIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62N|M2OyEQvF2= MlzaV2FPT0WU
human LK-2 cell NEHqeZJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MoTyTY5pcWKrdHnvckBw\iCqdX3hckBNUy1{IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62OFU6PCEQvF2= NGftdplUSU6JRWK=
human CTB-1 cell MVjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MVTJcohq[mm2aX;uJI9nKGi3bXHuJGNVSi1zIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT62O|A5OiEQvF2= NWnMUoc5W0GQR1XS
human NCI-H1581 cell MknZS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYnLW|M3UW6qaXLpeIlwdiCxZjDoeY1idiCQQ1mtTFE2QDFiY3XscEBoem:5dHigbY4h[SClZXzsJJZq[WKrbHn0fUBie3OjeTygTWM2OD1zLk[3OVUh|ryP M3z2TXNCVkeHUh?=
human COLO-800 cell NW\EcG9nT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYHJcohq[mm2aX;uJI9nKGi3bXHuJGNQVE9vOECwJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU44ODN6MjFOwG0> M4DLZXNCVkeHUh?=
human NB7 cell MVzHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M{m0dWlvcGmkaYTpc44hd2ZiaIXtZY4hVkJ5IHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT63OVI6PyEQvF2= MomwV2FPT0WU
human LAMA-84 cell NF;nSJhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NWnuWmlmUW6qaXLpeIlwdiCxZjDoeY1idiCOQV3BMVg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS55NUWyJO69VQ>? M4[xSnNCVkeHUh?=
human HCT-116 cells MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3TtfmlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWLUGxOkBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQDB7MEig{txO M4npOnNCVkeHUh?=
SK-UT-1 cell MlfIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MlrpTY5pcWKrdHnvckBw\iCqdX3hckBUUy2XVD2xJINmdGxiZ4Lve5RpKGmwIHGgZ4VtdCC4aXHibYxqfHliYYPzZZktKEmFNUC9NU45OTV|IN88US=> NEf0PZlUSU6JRWK=
human H4 cell MWjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MXvJcohq[mm2aX;uJI9nKGi3bXHuJGg1KGOnbHyg[5Jwf3SqIHnuJIEh[2WubDD2bYFjcWyrdImgZZN{[XluIFnDOVA:OS56MUW3PEDPxE1? MkjCV2FPT0WU
human CAL-51 cell NHjpPHdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NFftc4tKdmirYnn0bY9vKG:oIHj1cYFvKEODTD21NUBk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQDN6NEWg{txO MmDYV2FPT0WU
human LoVo cells MlTXVJJwdGmoZYLheIlwdiCjc4PhfS=> NF:zXWg4OiCq M3;VdWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTH;Wc{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUMYJie2WmIGfTWFghemWjZ3XueEBie3OjeTygTWM2OD1zLkmg{txO NIrzUnIzPTJ5MESwNy=>
human HN cell NWOxOnhFT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NH3OdldKdmirYnn0bY9vKG:oIHj1cYFvKEiQIHPlcIwh\3Kxd4ToJIlvKGFiY3XscEB3cWGkaXzpeJkh[XO|YYmsJGlEPTB;MT65NlUyKM7:TR?= M{OzWXNCVkeHUh?=
human L-363 cell NET3bJRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NYD0WnlMUW6qaXLpeIlwdiCxZjDoeY1idiCOLUO2N{Bk\WyuIHfyc5d1cCCrbjDhJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwQVEvQTVzMjFOwG0> NELOd5RUSU6JRWK=
human NCI-H747 cell NHPSe3ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M3zqWGlvcGmkaYTpc44hd2ZiaIXtZY4hVkOLLVi3OFch[2WubDDndo94fGhiaX6gZUBk\WyuII\pZYJqdGm2eTDhd5NigSxiSVO1NF0zNjB|M{WzJO69VQ>? M370ZXNCVkeHUh?=
human A498 cell M{\0e2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M{S2S2lvcGmkaYTpc44hd2ZiaIXtZY4hSTR7ODDj[YxtKGe{b4f0bEBqdiCjIHPlcIwhfmmjYnnsbZR6KGG|c3H5MEBKSzVyPUKuN|Y3QSEQvF2= M2jyO3NCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p53 / p21 / p-p38 / p38; 

PubMed: 23759594     


U2OS cells were treated for the indicated time with 1 µM ZM447439. Levels of p53, p21, phosphorylated p38 (p-p38) and total p38 were determined by immunoblotting.

pHistone H3 / CEM ; 

PubMed: 22359551     


Detection of phospho Histone H3(Ser10) in CEM and CEM/AKB4 cells treated for 24 hr with increasing concentrations of ZM447439 by western blotting. Shown are representative blots from three independent experiments.

BubR1 / MAD2 / Cyclin B1 ; 

PubMed: 26188358     


ZM induces mitotic slippage in AML cells. NB4 cells were incubated with different doses of ZM (0.5, 1, 2, 5 and 10 μM) or DMSO for 48h; Whole-cell extracts was subjected to immunoblotting by using indicated antibodies.

23759594 22359551 26188358
Immunofluorescence
p21; 

PubMed: 23759594     


U2OS cells were treated with 1 µM ZM447439 for 24 h. Nuclei were stained with Hoechst 33258. p21 was detected by immunostaining. Bar: 50 µm. 

p53; 

PubMed: 23759594     


U2OS cells were treated for the indicated time with 1 µM ZM447439. Levels of p53, p21, phosphorylated p38 (p-p38) and total p38 were determined by immunoblotting.

Aurora B / Survivin ; 

PubMed: 12719470     


Immunofluorescence images of prometaphase DLD-1 cells stained to detect Aurora B (green), Survivin (red), and DNA (blue) after exposure to ZM447439 for 1 h.

23759594 12719470

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

体外激酶实验:

使用杆状病毒表达体系,表达重组Aurora A和B作为氨基末端His6标记融合蛋白。使用Ni-NTA琼脂糖,通过亲和层析纯化Aurora A,使用CM Sepharose Fast Flow,通过离子交换层析纯化Aurora B。1 ng 纯化的重组酶加到反应中,反应中含 25 mM Tris-HCl, pH 7.5, 12.5 mM KCl, 2.5 mM NaF, 0.6 mM DTT, 6.25 mM MnCl2, 10 μM 肽底物, 10 μM ATP(Aurora A),5 μM ATP(Aurora B), 和0.2 μCi γ[33P]ATP (比活≥2,500 Ci/mmol), 然后在室温下温育60分钟。加入20% 磷酸反应终止,使用P30硝化纤维过滤器获得产物, 使用Beta 板TM计数器测定33P摄入率。
细胞实验:[2]
- 合并
  • Cell lines: BON, QGP-1和MIP-101
  • Concentrations: 0到5 μM
  • Incubation Time: 72小时
  • Method: 通过结晶紫染色测评细胞数。细胞接种在96孔板上,与1% 戊二醇混合。细胞用0.1% 结晶紫染色。用水冲洗未结合的染料。用0.2% Triton X-100溶解结合的结晶紫。使用ELISA读数器在570 nm处分析线性增加的细胞数。
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 103 mg/mL (200.54 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
30% PEG400+0.5% Tween80+5% propylene glycol
30 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 513.59
化学式

C29H31N5O4

CAS号 331771-20-1
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID