Panobinostat (LBH589)

目录号:S1030 别名: NVP-LBH589 中文名称:帕比司他

仅限科研使用

Panobinostat (LBH589, NVP-LBH589)是一种新型的,广谱HDAC抑制剂,无细胞试验中IC50为5 nM。Panobinostat (LBH589) 可诱导自噬和凋亡。Panobinostat 可以有效地破坏体内 HIV 的潜伏期。Phase 3。

Panobinostat (LBH589) Chemical Structure

CAS: 404950-80-7

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 953.44 现货
RMB 731.86 现货
RMB 2189.52 现货
RMB 5485.18 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Panobinostat (LBH589)发表文献336篇:

产品安全说明书

HDAC抑制剂选择性比较

相关HDAC产品

生物活性

产品描述 Panobinostat (LBH589, NVP-LBH589)是一种新型的,广谱HDAC抑制剂,无细胞试验中IC50为5 nM。Panobinostat (LBH589) 可诱导自噬和凋亡。Panobinostat 可以有效地破坏体内 HIV 的潜伏期。Phase 3。
靶点
HDAC (MOLT-4 cells) [1] HDAC (Reh cells) [1]
5 nM 20 nM
体外研究

LBH589诱导MOLT-4和Reh细胞凋亡,这种作用具有时间和剂量依赖性。而且,LBH589作用于MOLT-4细胞比作用于Reh细胞更有效。LBH589明显阻止MOLT-4 和Reh细胞的生长,处理48小时,具有剂量依赖性。与对照组细胞相比,用LBH589处理的细胞,在细胞周期的G2/M期的细胞数量增多2到3倍。LBH589与组蛋白H3K9和H4K8的乙酰化作用相关,LBH589也降低c-Myc的表达水平,具有剂量依赖性。LBH589也增强p21的表达水平。最低剂量的LBH589(10 nM)处理Reh细胞,最初增强c-Myc的表达水平,之后一直降低c-Myc的表达水平。此外,LBH589促进mRNA水平的促凋亡和DNA修复基因的大量增多。在GADD45G启动子作用下,LBH589诱导乙酰化的组蛋白H3 和H4水平的增多。[1]此外, LBH589抑制非小细胞肺癌细胞系生长,如人类H1299,L55和A549,IC50分别为5,11和30 nM;间皮瘤细胞生长,如人类OK-6和 Ok-5,IC50分别为5和7 nM;及小细胞肺癌细胞系生长,如人类RG-1和LD-T,IC50分别为4和5 nM。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HT29 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWO5b5cxOC1zMDFOwG0> Mlq2NE01KGR? MnfKbY5pcWKrdIOgZ4VtdCCpcn;3eIghcW5iYn;0bEB1cW2nLTDhcoQh\G:|ZT3k[ZBmdmSnboSgcYFvdmW{ MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyMke4OEc,OjZ5MEK3PFQ9N2F-
HepG2 M3LJ[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEWzVIExNTFyIN88US=> MYSwMVQh\A>? MY\pcohq[mm2czDj[YxtKGe{b4f0bEBqdiCkb4ToJJRqdWVvIHHu[EBld3OnLXTldIVv\GWwdDDtZY5v\XJ? MkDxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ5MEK3PFQoRjJ4N{CyO|g1RC:jPh?=
HT29 NV\mVXl2TnWwY4Tpc44hSXO|YYm= NFnTO4Q2OMLibl2= MnL1NlQuPzJiaB?= MWLpcoR2[2WmIHHjeIl3[XSrb36gc4Yh[2G|cHHz[UA{KGGodHXyJFQ5yqCqwrC= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjdyMke4OEc,OjZ5MEK3PFQ9N2F-
HepG2 NVLj[FZFTnWwY4Tpc44hSXO|YYm= NFjENIo2OMLibl2= NGfRR5gzPC15MjDo MWrpcoR2[2WmIHHjeIl3[XSrb36gc4Yh[2G|cHHz[UA{KGGodHXyJFI1yqCqwrC= NGLGWY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkewNlc5PCd-Mk[3NFI4QDR:L3G+
HCC827 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4D2VFUwPy53L{GwJI5O MnO2O|LDqGh? M1rGVWROW09? NXnsfXo3\W6qYX7j[ZMhfGinIHHueIlxem:uaX\ldoF1cX[nIHXm[oVkfCCxZjDldoxwfGmwaXK= NHHRZ5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[3OVQ5PCd-Mk[2O|U1QDR:L3G+
A549  NInYe2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHTSNWIyOC9zNT:yNEBvVQ>? NEW2Zlg4OsLiaB?= NV7MN3ZFTE2VTx?= MXjlcohidmOnczD0bIUh[W62aYDyc4xq\mW{YYTpeoUh\W[oZXP0JI9nKGW{bH;0bY5q[g>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZ5NUS4OEc,OjZ4N{W0PFQ9N2F-
NCI-H460  MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NI\XdmUyOC9{MD:zNEBvVQ>? NFHMSpM4OsLiaB?= NIjodXRFVVOR NYPiVI9F\W6qYX7j[ZMhfGinIHHueIlxem:uaX\ldoF1cX[nIHXm[oVkfCCxZjDldoxwfGmwaXK= NIr0N4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[3OVQ5PCd-Mk[2O|U1QDR:L3G+
J89GFP NFXjW|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX\zUXo1TE2VT9Mg MkTvSWM2OD12OT64OUDDuSBzMj62OUBvVQ>? NILtOno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW2N|U3QCd-Mk[1OlM2Pjh:L3G+
THP89GFP MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIX4XFRFVVORwrC= M3nETmVEPTB;MUmuN|QhyrFiNj60N{BvVQ>? Mnj3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3NkO1OlgoRjJ4NU[zOVY5RC:jPh?=
SK-NEP-1 NUDKNoI3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFz3S2oxNjBz4pETNVAvOCEQvF2= MknzNlQhcA>? MoqwSG1UV8Li NXz4cHE2UUN3ME23Ok4{PCCwTR?= MljrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
G401 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{P5VlAvODIkgKOxNE4xKM7:TR?= M2TJOlI1KGh? M3H6TmROW00EoB?= NELHUZpKSzVyPUG0N{4xOiCwTR?= NHyyTHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
SK-NEP-1 MVPD[YxtKF[rYXLpcIl1gSCDc4PhfS=> NXPpcVZDPTBibl2= MlH4NgKBmzRiZB?= MWPEUXNQyqB? Mkn0doVlfWOnczDj[YxtKHO3co\peoFtKGmwIHGgeIlu\SCmZYDlcoRmdnRibXHucoVz NYjKcFg4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
G401 M17GUmNmdGxiVnnhZoltcXS7IFHzd4F6 MVS1NEBvVQ>? NFrQUIwy6oDVNDDk MmPVSG1UV8Li MVny[YR2[2W|IHPlcIwhe3W{dnn2ZYwhcW5iYTD0bY1mKGSncHXu[IVvfCCvYX7u[ZI> NFjvXlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
SK-NEP-1 NXjNbnpWSXCxcITvd4l{KEG|c3H5 NGTDZW02OC9zMECgcm0> NFHucI0zPCCq NI\lVZFFVVORwrC= NXPEd5QxcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF5NkKxPUc,OjZzN{[yNVk9N2F-
G401 MmXXRZBweHSxc3nzJGF{e2G7 M1zv[lUxNzFyMDDuUS=> M2PoelI1KGh? NVfjXGxMTE2VT9Mg NYLweHhLcW6mdXPld{Bk\WyuIHHwc5B1d3OrczDpckBiKGSxc3Wt[IVx\W6mZX70JI1idm6nch?= NYj5TJlWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
SK-NEP-1 M1PFfWZ2dmO2aX;uJGF{e2G7 M2LDOlUxNzFyMDDuUS=> NHTXd5UzPCCq NYXsZ3VvTE2VT9Mg M4HZUZNpd3e|IITo[UBqdmS3Y4Tpc44hd2ZiRF7BJIZz[WevZX70ZZRqd25? NF7rbIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkG3OlIyQSd-Mk[xO|YzOTl:L3G+
G401 NEPYcIRHfW6ldHnvckBCe3OjeR?= MWC1NE8yODBibl2= MVOyOEBp MkjSSG1UV8Li M{HaVJNpd3e|IITo[UBqdmS3Y4Tpc44hd2ZiRF7BJIZz[WevZX70ZZRqd25? NXrnd4g4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
SK-NEP-1 MX;GeY5kfGmxbjDBd5NigQ>? MofCOVAwOTByIH7N MXSyOEBp NEDWRYdFVVORwrC= M1jxe4lv\HWlZYOgZ4VtdCCleXPs[UBlcXOxcnTlduKh NVL1T3BNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xO|YzOTlpPkK2NVc3OjF7PD;hQi=>
G401 NFu5PW5HfW6ldHnvckBCe3OjeR?= MlzUOVAwOTByIH7N NWrxU|c6OjRiaB?= M3W2RWROW00EoB?= MX\pcoR2[2W|IHPlcIwh[3mlbHWg[Il{d3KmZYNCpC=> MkTzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZzN{[yNVkoRjJ4MUe2NlE6RC:jPh?=
RPMI 8226 M{DXPWNmdGxiU4Xyeol3[WxiQYPzZZk> NX;1V5dXOi92L{[gcm0> M4LjNlQ56oDLaB?= MUPpcoR2[2W|IHGgd4lodmmoaXPhcpQh\GWlcnXhd4UhcW5idHjlJINmdGxiZ4Lve5Rp MnL6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
OPM2 NX7kPZRtS2WubDDTeZJ3cX[jbDDBd5NigQ>? M2fQRlIwPC94IH7N M3exRVQ56oDLaB?= NGjQb2xqdmS3Y3XzJIEhe2mpbnnmbYNidnRiZHXjdoVie2ViaX6geIhmKGOnbHyg[5Jwf3Sq M2C2eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MECwNlkzLz5{NkCwNFI6OjxxYU6=
U266 MlSwR4VtdCCVdYL2bZZidCCDc4PhfS=> NYn3cVBqOi92L{[gcm0> MU[0PQKBkWh? M{ftVolv\HWlZYOgZUB{cWewaX\pZ4FvfCCmZXPy[YF{\SCrbjD0bIUh[2WubDDndo94fGh? MkW3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyMECyPVIoRjJ4MECwNlkzRC:jPh?=
H929 MYHD[YxtKFO3co\peoFtKEG|c3H5 NUixXIZkOi92L{[gcm0> Mmq1OFjjiImq NGO2SmhqdmS3Y3XzJIEhe2mpbnnmbYNidnRiZHXjdoVie2ViaX6geIhmKGOnbHyg[5Jwf3Sq M1vDXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MECwNlkzLz5{NkCwNFI6OjxxYU6=
RPMI 8226  NFHaWYFCeG:ydH;zbZMhSXO|YYm= MV205qCKdk1? M1H6RlI1NzR6IHi= M{ftb4lv\HWlZYOgZ4VtdCCjcH;weI9{cXNiaX6gZUB1cW2nLXTldIVv\GWwdDDtZY5v\XJ? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjByMEK5Nkc,OjZyMECyPVI9N2F-
HCC827 NIfCfYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rHeFExKG6P MYO0PEBp MWfEUXNQ NF;pV4RmdmijbnPld{BkcXOybHH0bY4he2Wwc3n0bZZqfHoEoB?= NIfk[JA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUm0OFYyPyd-MkW5OFQ3OTd:L3G+
NCI-H23 M2Psfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWqxNEBvVQ>? M1G0fVQ5KGh? NVXBXHc6TE2VTx?= MlHO[Y5p[W6lZYOgZ4l{eGyjdHnuJJNmdnOrdHn2bZR6yqB? MkLiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV7NES2NVcoRjJ3OUS0OlE4RC:jPh?=
AML3 NUH1UmFWTnWwY4Tpc44hSXO|YYm= MUSwMVEh|ryP M1jDdlI1yqCq M4\uRolv\HWlZYOgSG5CKG[{YXft[Y51[XSrb39CpIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy NIjj[|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[xNlk1OSd-MkW2NVI6PDF:L3G+
ML-1 MWXGeY5kfGmxbjDBd5NigQ>? MWmwMVEh|ryP NWPkV5ZHOjUEoHi= NVHobHZGcW6mdXPld{BFVkFiZoLh[41mdnSjdHnvcuKhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NHfLVlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[xNlk1OSd-MkW2NVI6PDF:L3G+
RPMI-8226vr10  NFHseoxHfW6ldHnvckBCe3OjeR?= NWPBbo1xOC1zIN88US=> NUfGSGsxOjUEoHi= NGPKNIxqdmS3Y3XzJGRPSSCocnHncYVvfGG2aX;uxsBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZzMkm0NUc,OjV4MUK5OFE9N2F-
ML-1 NHXlSWlHfW6ldHnvckBCe3OjeR?= M3rBTFEh|ryP M1X0U|I1yqCq NX:weldZcW6lcnXhd4V{KGOjc4Dhd4UuOyCjY4Tpeol1gSB2LX\vcIQ> NWfCXlhORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2NVI6PDFpPkK1OlEzQTRzPD;hQi=>
RPMI-8226vr10  M1fLTWZ2dmO2aX;uJGF{e2G7 MYKxJO69VQ>? M3;SbFI1yqCq M1;ISolv[3KnYYPld{Bk[XOyYYPlMVMh[WO2aY\peJkhOi53LX\vcIQ> M{PidlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkGyPVQyLz5{NU[xNlk1OTxxYU6=
SK-N-BE (2) Ml3SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfUdXV3OjUkgJno M1:5RmlEPTB;MUC0MlDjiIoEsfMAjVcvQCCwTR?= M2nzVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C4PVE3Lz5{NUOwPFkyPjxxYU6=
SK-N-BE (2), PAN  MK NWHmZYF6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWqyOQKBkWh? MmDhTWM2OD1zMESuNQKBkcLz4pEJO{45KG6P NEniU|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUOwPFkyPid-MkWzNFg6OTZ:L3G+
SK-N-BE (2), MK  PAN NFLp[JhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVG4Z3p[OjUkgJno M{\3dWlEPTB;M{iyMlDjiIoEsfMAjVQ{NjJibl2= NWH3c3A{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFg6OTZpPkK1N|A5QTF4PD;hQi=>
SK-N-AS NVnkWZBLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYSyOQKBkWh? M2jjO2lEPTB;M{euNgKBkcLz4pEJNk41KG6P M3zjNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{C4PVE3Lz5{NUOwPFkyPjxxYU6=
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HepG2 NFzOSGNHfW6ldHnvckBCe3OjeR?= MlHaOVAwOTByIH7N NIeyRmgzPCCq M3LDT2ROW09? NVG2N|FJ\GWlcnXhd4V{KHSqZTDs[ZZmdHNib3[gdE1UXEGWMzDhcoQheC2Da4VCpC=> NXTrUppGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVM6PTZpPkK0NFk{QTV4PD;hQi=>
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HepG2 M4HNW2Z2dmO2aX;uJGF{e2G7 MmTWOVAwOTByIH7N MViyOEBp NHv4U|FFVVOR MkWy[I94dnKnZ4XsZZRmeyCEY3ytfGwh\XiycnXzd4lwdg>? MlS4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyOUO5OVYoRjJ2MEmzPVU3RC:jPh?=
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MV4-11 NXHuOYN2TnWwY4Tpc44h[XO|YYm= NXL0OZpZPTBibl2= Mn[4NkB1dyB{NDDodpM> M1HyUmlvcGmkaYTpc44hd2ZiSFTBR|YhcW5iaIXtZY4hVVZ2LUGxJINmdGy|IHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iCjbIDoZU11fWK3bHnuJIh6eGW{YXPleJlt[XSrb36gZZQhPTBibl2gZYZ1\XJiMjD0c{AzPCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? MmTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
MV4-11 NI\pNIdE\WyuIHP5Z4xmKGG{cnXzeEBie3OjeR?= MVizNEB1dyB3MDDuUS=> Mm[4NlQhcHK| M2GxfmNmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJG1XPC1zMTDj[YxteyCjc4Pld5Nm\CCjczDhZ4N2dXWuYYTpc44h[XRic4XiMWcyKHCqYYPlJIF1KDNyIITvJFUxKG6PIHHmeIVzKDJ2IHjyd{BjgSCycn;wbYRqfW1iaX;kbYRmKHO2YXnubY5oNWKjc3XkJIZtd3diY4n0c41mfHKrYzDt[ZRpd2R? Mn3pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
MV4-11 NHG3TG5CeG:ydH;zbZMh[XO|YYm= NYW0OY8xOzBibl2= NYjGU2pxOjRidH:gOFghcHK| MmP1TY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCPVkStNVEh[2WubIOgZZQhOzBibl2gZYZ1\XJiMkSgeI8hPDhiaILzJIJ6KEGwbnX4bY4hXi2SSTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfS=> MonuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5M{i5OVMoRjJ7N{O4PVU{RC:jPh?=
Assay
Methods Test Index PMID
Western blot DNMT1 / EZH2 caspase-8 / cleaved caspase-8 / Sp1 c-Myc / IRF4 Ac-H3 / cleaved caspase-3 / CCND1 / ID1 / ID2 / ID3 / ID4 / Synaptophysin / NeuroD1 RAD51 / BRCA1 / CHK1 / RPL13a H3K9AC / H3K18AC / H3K56AC / H3 / H4K8AC / H4K16AC / H4 / p21 / p27 / cleaved PARP 19279403 27738323 28915627 24244429 31071955
Immunofluorescence Synaptophysin / NACM α-tubulin / Acetyl-α-tubulin BiP ATF4 IRE1α / S724-IRE1α 28915627 29983882 23544167
Growth inhibition assay Cell viability 27738323
体内研究 LBH589作用于患肺癌和间皮瘤动物模型,明显降低肿瘤生长,与对照组相比平均降低62%。LBH589作用于免疫活性鼠和严重联合免疫缺陷鼠效果差不多,说明LBH589抑制肿瘤生长与免疫学无关。LBH589按20 mg/kg剂量腹腔注射,每周持续5天,导致在实验最后肿瘤生长平均下降70%。与相应的对照组肿瘤相比,LBH589作用于H526衍生的肿瘤下降53%,作用于BK-T衍生的肿瘤下降81%, 作用于RG-1衍生的肿瘤下降76%,作用于H69衍生的肿瘤下降 70%。在相同条件和剂量的情况下,LBH589 作用于NSCLC和Meso衍生的移植瘤,导致SCLC衍生的肿瘤中有两种衰退,伴随着BK-T衍生的平均肿瘤尺寸从实验开始时的296 mm3降低到实验结束时的116 mm3,RG-1衍生的平均肿瘤尺寸从实验开始时的185mm3降低到实验结束时的86 mm3[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[1]
  • Cell lines: MOLT-4细胞和Reh(前体B细胞)
  • Concentrations: 50 nM
  • Incubation Time: 48小时
  • Method: 没有处理的细胞和LBH589处理的细胞[人类急性成淋巴细胞性白血病MOLT-4(T 细胞)和Reh(前体-B细胞)]用膜联蛋白V和碘化丙啶染色,然后加入膜联蛋白V-FITC细胞凋亡检测试剂盒I。通过流式细胞仪测定细胞凋亡和不能存活细胞的百分比。用CyAn ADP Violet细胞计数器收集至少5×104个细胞。根据所有的膜联蛋白V-阳性和膜联蛋白V/PI-阳性细胞细胞计算凋亡百分比,根据所有的膜联蛋白V-阳性和PI-阳性及膜联蛋白V/PI-阳性细胞计算细胞活力丢失百分比。
动物实验:[2]
  • Animal Models: 携带10×106个M30细胞或5×106个A549细胞的SCID鼠
  • Dosages: 10 mg/kg, 20 mg/kg
  • Administration: 腹腔注射

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+48% PEG 300+2% Tween 80+ddH2O

5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 349.43
化学式

C21H23N3O2

CAS号 404950-80-7
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04341311 Active not recruiting Drug: Marizomib|Drug: Panobinostat Diffuse Intrinsic Pontine Glioma|Pediatric Brainstem Glioma|Pediatric Brainstem Gliosarcoma Recurrent|Pediatric Cancer|Pediatric Brain Tumor|Diffuse Glioma Dana-Farber Cancer Institute|Celgene|Secura Bio Inc. August 10 2020 Phase 1
NCT03632317 Withdrawn Drug: Panobinostat|Drug: Everolimus Glioma|Diffuse Intrinsic Pontine Glioma University of Michigan Rogel Cancer Center October 2019 Phase 2
NCT03982134 Withdrawn Drug: PDR001|Drug: Panobinostat Melanoma|Non Small Cell Lung Cancer Muhammad Furqan|Novartis Pharmaceuticals|University of Iowa September 2019 Phase 1
NCT04326764 Active not recruiting Drug: Panobinostat Acute Myeloid Leukaemia (AML)|Myelodysplastic Syndromes (MDS) Goethe University|Jan J. Cornelissen (HOVON-SAKK)|Sebastian Giebel (PALG) July 24 2018 Phase 3
NCT03515915 Unknown status -- Patients With Recurrent or Refractory Multiple Myeloma University Hospital Montpellier|Poitiers University Hospital April 23 2018 --

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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操作手册

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常见问题及建议解决方法

问题 1:
How to reconstitute the compound for in vivo mice study?

回答:
We recommend the vehicle is 2 % DMSO, 2 % Tween 80, 48%PEG300, 48% water. The compound is first dissolved in DMSO, then add Tween, PEG300, water in sequence.

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