Vorinostat (SAHA, MK0683)

目录号:S1047 别名: SAHA, MK0683, suberoylanilide hydroxamic acid

Vorinostat (SAHA, MK0683) Chemical Structure

Molecular Weight(MW): 264.3

Vorinostat (suberoylanilide hydroxamic acid, SAHA)是一种HDAC抑制剂,无细胞试验中IC50为~10 nM。

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客户购买Selleck的此次产品后发表的文献100篇:

客户使用该产品的20个实验数据:

  • Activity of vorinostat in Jurkat and Peer T-ALL cell lines in an MTT cell viability assay. Cells were treated with increasing drug concentrations for 72 h. The data are plotted as the mean % of DMSO-treated control cells against the corresponding drug concentration. The error bars are the standard error. For each drug and cell line, 3-4 independent experiments were performed with 6 replicates at each drug concentration.

    Nature 2011 471, 235-9. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Differential effects of HDAC inhibitors on histone and tubulin acetylation. Immunofluorescence analysis of histone H3 (K9ac/K14ac) and tubulin acetylation in HeLa cells treated for 4 h with vehicle, SAHA (10 μM), tacedinaline (50 μM), PCI-24781 (20 μM. (a) Mapping of histone acetylation in K562 cells treated with HDAC inhibitors by LC-MS/MS. Cells were treated with TSA (10 μM), SAHA (5 μM), PCI-24781 (2 μM), tacedinaline (50 μM) for 6 h. Histones were extracted from cells and acetylated peptides were quantified after isobaric tagging.

    Nat Biotechnol 2011 29, 255-265. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Western blot analysis of histone H3 acetylation in the spleen of untreated and vorinostat-treated hNF-E2 tg mice (n = 4 of each genotype).

    J Exp Med 2012 209, 35-50. Vorinostat (SAHA, MK0683) purchased from Selleck.

    SAHA down-regulates c-FLIP expression and increases the sensitivity of CaP cells to undergo apoptosis in the presence of bicalutamide (A) Bar graph illustrating the sub-G0/G1 cell population in 22Rv1 (left panel) and LNCaP (right panel) cells in the absence and presence of 10μM bicalutamide, administered as a single agent or in ombination with increasing concentrations of the HDAC inhibitor, SAHA. (B) Immunoblots demonstrating the single agent activity of bicalutamide or SAHA, or effect upon their combined administration on the expression of c-FLIP and/or the processing of PARP. Equal protein loading was confirmed by GAPDH. (C) Bar graph illustrating the effect of bicalutamide and SAHA upon the induction of caspase-8 or caspase-3/7 activity in 22Rv1 cells (left panel) and LNCaP cells (right panel). (D) Immunoblots characterizing the impact of SAHA and/or bicalutamide upon the induction of PARP cleavage, in the absence or presence of the pan-caspase inhibitor, ZIETD. ZIETD was incubated with the cells for 12 h prior to the experiment. All data points shown in (A) and (C) represent the mean ± S.E.M. value, calculated from four independent experiments.

    Clin Cancer Res 2012 18, 3822-33. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Analyses of efficacy, potency and IC50 of HDAC inhibitors point toward HDACs 1–3 as relevant candidates for beta cell protection. Ranking and raw data on ITF drugs (Table 3) and commercial HDAC inhibitors (Table 4) underlying the heat maps of Fig. 1 (A and B). The HDAC inhibitor compounds were tested using a HDAC activity kit and recombinant proteins to determine IC50 values on each individual HDAC (right part of the table). Each drug was further tested using Real-Time Cell Analysis (RTCA) to score their maximal effective concentration (ECmax) as well as the corresponding rescue percentage of cytokine treated INS-1 cells. The drugs were ranked according to % rescue. * DMSO alone controls were included in each experiment, but not shown here. The results indicate that the highest concentrations of DMSO used here (1:1,000) were slightly potentiating the proliferation of the cells. The effects observed in this group of compounds were ascribed to the DMSO.?

    Diabetologia 2012 55, 2421-2431. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Suberoylanilide hydroxyamic acid (SAHA) preserves CFP (+) expression in mouse optic nerves (MONs) from older mice. (a) CFP (+) mitochondria were longer and thicker in MONs from 12-month-old Thy-1 CFP mice (inset; scale bar = 2 μm). Oxygen–glucose deprivation (OGD) consistently reduced CFP pixel intensity in 12-month-old MONs despite longer and brighter CFP ( +) mitochondria (yellow arrows). (b) Blockade of AMPA/kain ate receptors with NBQX (30 μM), or pan-Histone deacetylase (HDAC) inhibition with SAHA (5 μM), effectively preserved CFP pixel intensity during OGD. The preservation of CFP (+) mitochondria correlates with protection of the CAP area during OGD and profound recovery.

    J Neurochem 2012 123 Suppl 2, 108-15. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • D and E, Regression of IL-13Ra 2-negative pancreatic tumors treated with SAHA and/or IL-13-PE. Mice were treated daily with i.p. injection of SAHA (25 mg/kg) from day 4 after tumor implantation for two weeks followed by i.t. injection of IL-13-PE (100 μ g/kg) from day 5 for two weeks. F and G, Regression of IL-13Ra 2-posotive pancreatic tumors (HS766T and MIA-PaCa2) treated with SAHA and/or IL-13-PE. The schedule of treatment was similar as in panel D and E. Statistical significances are shown by *: P < 0.05,†: P < 0.001.

    J Transl Med 2011 9, 37. Vorinostat (SAHA, MK0683) purchased from Selleck.

    IL-13Rα2 expression is upregulated in pancreatic tumors but not in organs of mice after treatment with HDAC inhibi tor, SAHA. A, qRT-PCR of human IL-13Rα2 in implanted pancreatic tumors after SAHA and IL-13-PE treatment. Tumors were harvested next day after IL-13-PE treatment and total RNA extracted. Data shown is ratio of human IL-13Rα2/β-actin expression and multiplied by 1000 for convenience. Bars , SD of triplicate determinations. B, qRT-PCR of mouse IL-13Rα2 in mice organs after SAHA and IL-13-PE treatment. Tissues were harvested at the same time point as in panel A and total RNA extracted. Data shown is ratio of mouse IL-13Rα2/β-actin expression and multiplied by 100 for convenience. C, IHC of human IL-13Rα2 in implanted pancreatic tumors after SAHA and IL-13-PE treatment. D, IHC of mouse IL-13Rα2 in mice organs after SAHA and IL-13-PE treatment. Liver, brain, kidney, pancreas, lung and spleen were fixed for immunostaining of mouse IL-13Rα2 as visualized by Alexa555. Nucleus was counterstained by DAPI.

    J Transl Med 2011 9, 37. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Proliferation o f HT29 and HCT116 cells after 48 h of butyrate or SAHA treatment. Proliferation measurements using the xCEL Ligence RTCA DP instrument (Roche) in cells treated with increasing doses of butyrate or SAHA, compared with control medium cells (0) (*P < 0.05). The mean ?SEM of three cell culture replicates is shown.

    Mol Carcinog 2012 52, 459-74. Vorinostat (SAHA, MK0683) purchased from Selleck.

    SAHA induces the down-regulation of cFLIP expression and promotes TRAIL-mediated activation of a mitochondrial-operated apoptotic pathway. a BT-474 cells were treated as indicated in Fig. 1 and the cells were then harvested to analyse caspase-8, Bid, caspase-9, caspase-3 and PARP expression, using GAPDH as the protein loading control. b BT-474 cells were treated with SAHA for 8 h at the doses indicated and subsequently, the cells were harvested and the apoptosis-related proteins were analysed in western blots as described in the "Materials and Methods". The results shown are representative of at least three independent experiments

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Treatment with SAHA sensitizes breast cancer cells to TRAILinduced apoptosis. BT-474 and MDA-MB-231 cells were incubated for 8 h with SAHA at the doses indicated. After this time, TRAIL (500 ng/ml) was added to some of the cultures for an additional 15 h. Apoptosis was measured as the percentage of cells with subG1 DNA content as described in the "Materials and methods". The results show the mean and S.E.M. of three independent experiments

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

    SAHA facilitates the Itch/AIP4-independent proteasomal degradation of cFLIP proteins in breast cancer cells. a BT-474 cells were treated with SAHA (5 μM) for 8 h in the presence or absence of the proteasome inhibitors MG132 or epoxomicin (at the doses indicated), and they were then harvested and the cFLIP content was analysed in western blots. b BT-474 cells were transfected with either the siRNA oligonucleotides targeting Itch/AIP4 or the non-targeting RNA oligonucleotide (scrambled) for 48 h as described in the "Materials and Methods". Subsequently, the cells were treated with SAHA (5 μM) for 8 h and then cFLIP and Itch/AIP4 expression was analysed in immunoblots

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • cFLIP down-regulation following SAHA treatment is responsible for the sensitization of breast cancer cells to TRAIL. a BT-474 cells were transfected with either different siRNAs directed against the cFLIP isoforms or the non-targeting RNA oligonucleotide (scrambled) as described in the "Materials and Methods". After 24 h, the cells were treated with TRAIL (500 ng/ml) for a further 15 h and apoptosis was then measured as the percentage of cells with subG1 DNA content. The results show the mean and S.E.M. of three independent experiments. To verify protein knockdown, cells were harvested 24 h after transfection and the cFLIP levels were assessed in immunoblots. b. BT-474 cells overexpressing Cflipl (pBABE-FLIPL) or control cells (pBABE) were treated with SAHA for 8 h at the doses indicated. After this time, TRAIL (500 ng/ml) was added to some cultures for 15 h. Apoptosis was measured as the percentage of cells with subG1 DNA content and the results show the mean and range of two independent experiments. Western blots show the cFLIP levels after SAHA treatment in pBABE and pBABE-FLIPL cells.

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Vorinostat inhibits the tumor growth. NOD ⁄ SCID-B2m-/-mice were injected s.c. with 1×106 MyLa2059 cells into both flanks. When the mice had developed established palpable tumors treatment was initiated (day 1). The mice received either vehicle (black line) or 60 mg ⁄ kg vorinostat (grey line) i.p. five days a week. In total, there were four mice in the group receiving vehicle and three mice in the group receiving vorinostat. The length and width of the tumors were measured continuously until the experiment was terminated on day 20 post treatment initiation. The tumor volume was calculated using the formula V = (a×b2)/2, where a defines the length (mm) and b the width (mm) of the tumor. The histogram shows the average tumor volume per flank ± SEM. * denotes a significant difference (P < 0.05) in the average tumor volume per flank between mice treated with vehicle and vorinostat at the given time-point both by using a one-tailed two samples test with Welch, scorrection and a Mann-Whitney U test.

     

     

    Exp Dermatol 2010 19, 1096-1102. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • (a) Western blot of whole cell extracts demonstrates SAHA and NaB treatment result in increased levels of acetylated histone H3 compared to vehicle controls. (b) Light micrographs of HDACi/vehicle treated cultures illustrate the paucity of neurospheres in HDACi-treated cultures. (c) Quantitative analysis confirms SAHA and NaB inhibit the formation of neurospheres (diameter ≥ 50 μm) and increase the prevalence of small cell clusters (≥ 4 cell, diameter < 50 μm). (d) Flow cytometry using LIVE/DEAD cell stain confirms SAHA and NaB are minimally toxic compared to vehicle controls (n = 3). Scale bar = 100 μm.

    BMC Neurosci 2011 12, 50. Vorinostat (SAHA, MK0683) purchased from Selleck.

    (a) EdU flow cytometry of live cell-gated adult mouse NSCs demonstrates SAHA (**p < 0.01) and NaB (***p < 0.001) significantly inhibit DNA synthesis. (b) Analysis of DNA content by flow cytometry reveals significant accumulation of adult mouse NSCs in G1phase of the cell cycle following SAHA (*p < 0.05) and NaB (*p < 0.05) treatment. (c) Correspondingly the proportion of cells in G2/M phase is significantly reduced following SAHA (*p < 0.05) and NaB (*p < 0.05) treatment. (d) Fluorescence micrographs of HDACi/vehicle treated adult NSCs pulse-labeled with EdU for flow cytometry. Scale bar = 50 μm. Statistical comparisons performed using one-way ANOVA with post hoc Newman-Keuls multiple comparison tests.

    BMC Neurosci 2011 12, 50. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • (a) Immunofluorescence labeling of NeuN, GFAP and Olig2 cell fate markers reveals reduced expression of GFAP (*p < 0.001) and Olig2 (*p < 0.05) in adult mouse NSCs treated with SAHA for 48 hours under proliferation culture conditions and then induced to differentiate for 14 days in culture (two-way ANOVA with Bonferroni post-tests). (b) Western blot of β-catenin protein in nuclear and cytoplasmic fractions of adult mouse NSCs treated with HDACi/vehicle control under proliferation culture conditions. Gapdh and lamin A/C blots are included as loading controls. (c) SAHA and NaB do not significantly alter nuclear/cytoplasmic levels of β-catenin in adult mouse NSCs. Histogram of β-catenin Western blot signal intensities normalized to loading controls (n = 4).

    BMC Neurosci 2011 12, 50. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Morphological changes and histone H3 phosphorylation of HB cells treated with a combination of VX-680 and SAHA. HUH6 and HepT1 were incubated with VX-680 (6 μM) and SAHA (0.5 μM). Nuclei diameter (a) and cell diameter (b) were determined 72 h later by DAPI staining and microscopy. Data represent mean±SD of the diameters from 20 cells in each experiment. (* Two-way ANOVA, Bonferroni test, p \0.05). c Western blot analysis on HUH6 and HepT1 cells were carried out with an anti-phospho-Histone H3 (Ser 10) antibody (p-H3) 24 h after incubation with VX-680 (10 μM), SAHA (0.2 μM) or a combination of both. Controls were left untreated. Western blot analysis showed a decrease in p–H3 after treatment with VX-680 ( lane 2 ) relative to controls ( lane 1 ) and an increase when SAHA was added ( lane 3 ). For the combination of VX-680 and SAHA (lane 4 ) no p-H3 was detected.

    Pediatr Surg Int 2012 28, 579-89. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Vorinostat promotes C2C12 differentiation.In the image, the red is the myotube indicating differentiation ,the blue is the nuclei.

     

     

    Dr. Akihiko Yoshimura of Akihiko Yoshimura. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Western blot analysis of Acetyl-H3 and H3. 0-20μM SAHA was added.

     

     

    Dr. Zhang of Tianjin Medical University. Vorinostat (SAHA, MK0683) purchased from Selleck.

产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述 Vorinostat (suberoylanilide hydroxamic acid, SAHA)是一种HDAC抑制剂,无细胞试验中IC50为~10 nM。
特性 Vorinostat是广谱HDAC活性抑制剂,抑制I和II类酶 。
靶点
HDAC [1]
(Cell-free assay)
~10 nM
体外研究

Vorinostat是小分子量(<300)线性肟酸,抑制HDAC活性,包括乙酰化组蛋白和非组蛋白的积累,抑制培养细胞的增殖,且抑制肿瘤生长。Vorinostat通过结合到酶的活性位点而抑制 HDAC活性。Vorinostat 抑制多种转化细胞增殖(包括 淋巴瘤,多发性骨髓瘤,白血病,和非小细胞肺癌),与对照组相比抑制生长达50% 时的浓度为0.5 到10μM。Vorinostat作用于淋巴瘤和白血病细胞,包括 Burkitt, B-细胞急性淋巴细胞白血病 (B-ALL), MCL, DLBCL, ATL和T-细胞,不同程度抑制细胞增殖。除了抑制转化细胞增殖, Vorinostat 也抑制正常细胞增殖,通过比较Vorinostat 作用于一组细胞系–正常人非纤维细胞(WI-38)和 SV40巨大T抗原转化WI-38(VA-13细胞)而得到证明。Vorinostat 抑制两种类型细胞增殖,这种作用存在剂量依赖性。但发现Vorinostat 对转化细胞具有选择毒性,包括使肿瘤细胞死亡,而对正常细胞只起抑制作用,不会使其死亡。[1] Genistein 和Vorinostat 联用效果比5-aza和Vorinostat 联用作用于诱导细胞死亡有效很多。Genistein和Vorinostat联用作用于ARCaP-E使增殖降低 80 %,作用于ARCaP-M细胞,增殖降低60 %以上。Genistein和Vorinostat 联用抑制细胞增殖具有协同效应。作用于ARCaP-E 细胞(820个基因 诱导和1046个基因抑制) ARCaP-M 细胞,Vorinostat比Genistein 影响更多基因。[2] Vorinostat作用于肿瘤细胞表面,提高Fas蛋白水平,这种作用存在剂量依赖性,且按0.75 μM剂量处理,提高达到一个稳定水平。[3] Vorinostat 抑制肿瘤细胞生长,包括NB4, H460 和HCT-116,IC50分别为0.7μM, 3.4μM和1.2 μM。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A20 M3m3PGtqdmG|ZTDBd5NigQ>? M2nnbFEhcA>? NGXx[odFVVOR M3L1boFvfGlvSFTBR|Eh[WO2aY\peJkhf2m2aDDJR|UxKG:oIECuNVEzKM7:TR?= NUTiPXdiOTF7NkC0PFk>
Friend leukemic cell MlT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXu1NEDPxE1? NFv4Nnc4OiCq M2XodmROW09? NFvKdYtKSzVyPUCuPVkh|ryP M3TZWVEzOTB7OUGz
K562 NIPJ[ZJMcW6jc3WgRZN{[Xl? NIXBOHI3KGh? MXvEUXNQ NUS2cHNxUW6qaXLpeIlwdiCxZjDoeY1idiCqaYP0c45mKGSnYXPleJlt[XOnIDjtbZh1fXKnIH;mJGhFSUNzIHHu[EBJTEGFMjmge4l1cCCLQ{WwJI9nKDBwMEGg{txO MYGxNlQyQTN6MB?=
HT1080 NGTjeW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrNXpBvPzJiaB?= NGXsU|ZFVVOR MnrYTWM2OD1{LkSg{txO MYCxNlI4ODF5NR?=
H1299 M2W3ZWtqdmG|ZTDBd5NigQ>? MU[yNEBucW5? M1y5O2ROW09? M2HJU2lvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBJTEGFIHXufplu\SC5aYToJGlEPTBib3[gNE4yQTRizszN M1jWflE1PTJzNEKy
H1299 MmnUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jkTlczKGh? NE\PV29FVVOR MnXNTWM2OD15LkK0JO69VQ>? MmezNVQ2OjF2MkK=
HeLa M164TWtqdmG|ZTDBd5NigQ>? MoL1N|AhdWmw MkLHTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiaHnzeI9v\SCmZXHj[ZR6dGG|ZTD3bZRpKEmFNUCgc4YhOC5{ODFOwG0> NH3FO2cyPTZyM{m0PS=>
LOX-IMVI M1:5NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MljLOFghcA>? NYnzOoZCUUN3ME2xMlMh|ryP MYKxOVcyPTR5MB?=
NCI-H226 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYf6OVF5PDhiaB?= NVy1fI5LUUN3ME2yMlYh|ryP MX[xOVcyPTR5MB?=
RXF-631L MoroS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXe0PEBp NW\Me2lFUUN3ME2yJO69VQ>? MkT1NVU4OTV2N{C=
SNB-78 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXm0PEBp NVnt[|I3UUN3ME2xOkDPxE1? NWrYfnd3OTV5MUW0O|A>
St-4 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3;rNFQ5KGh? MYTJR|UxRTVwMjFOwG0> NWf3XlNkOTV5MUW0O|A>
ARP1 MmjLSpVv[3Srb36gRZN{[Xl? M3jWNlIh|ryP Mm\yOkBp MVLJcoR2[3Srb36gc4YhWDJzV1HGNUBo\W6nIHX4dJJme3Orb36= MlnoNVQ4OzR6ME[=
CEM MXPLbY5ie2ViQYPzZZk> MXmxJIg> M13ORWROW09? Mlz4TY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLkOzJO69VQ>? NHToVYsyPjR{MEC2OC=>
HCT15 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MomyOFghcA>? NFjSVplIUTVyPUCuPFIh|ryP M2jVdlE3PzJ|MkK3
NCI-H23 M1fUW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7yOFghcA>? NFq2Ro5IUTVyPUCuPVIh|ryP NHHnNHQyPjd{M{KyOy=>
GM15850 NX3zcWVGU2mwYYPlJGF{e2G7 NEHMTJQzNjVizszN MVW5OkBp NHjXOZFFVVOR MV;Jcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDsfZNqdmViMUSgZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV? M2jpeFE3QTJzM{[3
GM15850 NIjXNZdMcW6jc3WgRZN{[Xl? NF;kNlczNjVizszN MXW5OkBp NUf2flFbTE2VTx?= M2TZe2lvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFi0JIx6e2mwZTC1JIFk\XS7bHH0bY9vKG:oIF\YUkBo\W6n NIjrNJUyPjl{MUO2Oy=>
GM15850 NHPrSGVMcW6jc3WgRZN{[Xl? M4fRXVIvPSEQvF2= NUXoWHZOQTZiaB?= MnTkSG1UVw>? M{G2W2lvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFi0JIx6e2mwZTCxNkBi[2W2eXzheIlwdiCxZjDGXG4h\2WwZR?= M3;wSlE3QTJzM{[3
GM15850 MVzLbY5ie2ViQYPzZZk> MkfYNk42KM7:TR?= M2nYUVk3KGh? NUizVVI1TE2VTx?= M4DXO2lvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFizJIx6e2mwZTC5JIFk\XS7bHH0bY9vKG:oIF\YUkBo\W6n MXWxOlkzOTN4Nx?=
GM15850 MWfLbY5ie2ViQYPzZZk> M2PWbFIvPSEQvF2= NWPpe242QTZiaB?= MXjEUXNQ NIPO[YhKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIOEBtgXOrbnWgPEBi[2W2eXzheIlwdiCxZjDGXG4h\2WwZR?= M{H2OFE3QTJzM{[3
GM15850 MkTKT4lv[XOnIFHzd4F6 MoDXNk42KM7:TR?= MoDNPVYhcA>? NH\ZV2dFVVOR NVzOd|M4UW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFE3KGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l MnjtNVY6OjF|Nke=
U937 NE\0XHpHfW6ldHnvckBCe3OjeR?= NWrO[mFXPSEQvF2= M1HxdVI1KGh? NYLn[YJDTE2VTx?= MVXD[YxtKGO7Y3zlJIFzemW|dDDifUBi[2O3bYXsZZRqd25iYYSgV{9IOi:PIIDoZZNm NX3VVmFYOTdyMES3NVg>
H661 MmDTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfRbpZjPDhiaB?= NITMPVFKSzVyPUGg{txO MW[xO|E2PzByOR?=
U937 NULVOZdpTnWwY4Tpc44hSXO|YYm= NU\vWYxLPSEQvF2= M4S1W|I1KGh? NHXSeoVFVVOR Ml7ETY5kemWjc3WgbY4hcGm|dH;u[UBJOyCjY3X0fYxifGmxbh?= MX2xO|Q1Pzd3MB?=
U937 NFzCcIRHfW6ldHnvckBCe3OjeR?= MXOxJO69VQ>? Mn3lNlQhcA>? M{nVTGROW09? M1TWV2Vn\mWldDDvckBidHCqYTD0eYJ2dGmwIHHj[ZR6dGG2aX;u NHu3cWUyPzR2N{e1NC=>
RAW264.7 MVLGeY5kfGmxbjDBd5NigQ>? NXXzNnpLOTBizszN NIHHVFczPGh? NGCwNWp4[XSnch?= M1voNGlvcGmkaYTpc44hd2ZiTGDTMZN1cW23bHH0[YQhXE6IYXzwbIEheHKxZIXjeIlwdiC5aYToJGlEPTBib3[gNE44PiEQvF2= M{Xse|E4PDd5NUG4
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Human microvascular epithelial cell MXrDfZRwfG:6aXOgRZN{[Xl? NX:zdJF[PTBizszN MV[3NkBp M{fVVGROW09? M3:2OGlEPTB;MT6zJO69VQ>? NFnldXEyQDR7NES2Ny=>
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HEK293 MoeyT4lv[XOnIFHzd4F6 NGPNPXY{KGh? MlXjTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDISGFEPiCneIDy[ZN{cW:wIIfpeIghUUN3MDDv[kAxNjBzOTFOwG0> MlfGNVg2PTh4Nkm=
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RAW264.7 MVjGeY5kfGmxbjDBd5NigQ>? MUSyJO69VQ>? MlPDN{42KGh? NXHa[m5VTE2VTx?= NWDtU5FOSW62aX3hcIFzcWGuIHHjeIl3cXS7IHHnZYlve3RiUHzhd41w\Gm3bTDmZYxkcXCjcoXtJIlv\mWldHXkJJdqfGhiSVO1NEBw\iByLkGxJO69VQ>? NVfQZ3FGOTh4NES5Olk>
NB4 M3vnO|Q5cA>? M3vuPGROW09? NXqwPJR[UUN3ME2wMlYh|ryP M2PkcFE5PzJ|M{S5
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HeLa M1TYS2tqdmG|ZTDBd5NigQ>? MV2yJO69VQ>? MnfBN|AhdWmw MUjEUXNQ NILrTYVKdmirYnn0bY9vKG:oIFjERWMzKGmwIHj1cYFvKEinTHGgZ4VtdHNid3n0bEBKSzVyIH;mJFAvOSEQvF2= M4rIOlE6ODh2Mkm0
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IGROV1 MoXxT4lv[XOnIFHzd4F6 Ml7NNVAh|ryP MnOxOEBp M13CfmROW09? NFG2OnVKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaHnzeI9v\S2KNDDhZ4V1gWyjdHnvci=> NF;zUGMyQTB6NEK5OC=>
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T24 MXjLbY5ie2ViQYPzZZk> M4TNWFMhcA>? M3\QU2lv\HWldHnvckBw\iCqaYP0c45mKEh|IHHj[ZR6dGG2aX;uJJdqfGhiRVO1NEBw\iByLkGyJO69VQ>? NGDxUWkyQTB6NEO5OS=>
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SKMES1 M{HBVGN6fG:2b4jpZ{BCe3OjeR?= NUjWW4FjPzJiaB?= MkP1SG1UVw>? MnfUTWM2OD1{LkSyJO69VQ>? M3fhfFE6ODl|OEi0
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SHSY5Y Ml;TR5l1d3SxeHnjJGF{e2G7 MXSxNEDPxE1? MoPxOFghcA>? M4PS[2ROW09? MlfyTWM2OD1{IN88US=> MlvONVk{PDRzN{W=
DMS114 Mlj1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\KWVQ5KGh? NFPzTnFIUTVyPUKuPUDPxE1? NX;TTY1WOTl2MUmyNFU>
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HeLa Mom5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnr1O|IhcA>? MWHEUXNQ Ml;1TWM2OD1yLkS2JO69VQ>? MVSxPVQ1OTh2Nh?=
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CFBE41o- NGj1R5JMcW6jc3WgRZN{[Xl? M2\kd|Uh|ryP MU[4JIg> MnTNSG1UVw>? NYK5V496UW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHj5dIVz[WOndInsZZRqd25ib3[gbIl{fG:wZTDINy=> NXLUW5B5OTl7Nk[3PFk>
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CFBE41o- MlfjT4lv[XOnIFHzd4F6 NH7ZN5oyKM7:TR?= M4HHXFEhcA>? MULEUXNQ NXHZdIh[UW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJGNHXFJicILveIVqdiCuZY\lcC=> NVrLN3F3OTl7Nk[3PFk>
Primary bronchial epithelial cell MWTLbY5ie2ViQYPzZZk> NGjB[2EyKM7:TR?= M2rQNVI1KGh? MXvEUXNQ M4TZNmlvcGmkaYTpc44hd2ZiSFTBR|ch[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJI12fGGwdDDG[IVtfGF3MEigR2ZVWiCycn;0[YlvKGGyaXPhcEB{fXKoYXPlJIxw[2GuaYrheIlwdg>? NFm5fHIyQTl4Nke4PS=>
CFBE41o- NFjUd5JMcW6jc3WgRZN{[Xl? NInkNZEyKM7:TR?= NXHtcoZ6PSCmYYnz M1vOb2ROW09? MlrITY5pcWKrdHnvckBw\iCKRFHDO{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDzeIFjcWyrenH0bY9vKG:oIF\k[Yx1[TVyODDDSnRTKHC{b4TlbY4> NXfzOpk2OTl7Nk[3PFk>
CFBE41o- MorhT4lv[XOnIFHzd4F6 NIK3UYEyKM7:TR?= M4LjOFI1KGh? NHzDclRFVVOR NXjvVI0yUW6qaXLpeIlwdiCxZjDISGFEPyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBH\GWudHG1NFghS0[WUjDwdo91\WmwIITyZYZncWOtaX7n MXSxPVk3Pjd6OR?=
CFBE41o- MWDLbY5ie2ViQYPzZZk> NX22bmpyPSEQvF2= MWCyOEBp MV7EUXNQ MlTzTY5pcWKrdHnvckBw\iCKRFHDO{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDyZZRmKG:oIH3heJVz[XSrb36gc4YhS0[WUjDwdo91\WmwIITvJIl1eyCpbInjc4Zwem1? NYK0OI5WOTl7Nk[3PFk>
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CFBE41o- MVXLbY5ie2ViQYPzZZk> MX[1JO69VQ>? NHzTcngzPCCq MYDEUXNQ NUXnS3hsUW6qaXLpeIlwdiCxZjDISGFEPyCjc4Pld5Nm\CCjczDjc5Jz\WO2aX;uJI9nKG23dHHueEBH\GWudHG1NFghS0[WUjD0doFn\mmla3nu[{B1dyClZXzsJJN2em[jY3WgZZMh\2y7Y3;mc5Ju NXzYNVVOOTl7Nk[3PFk>
Primary bronchial epithelial cell NVq4eG5bU2mwYYPlJGF{e2G7 MnXxOUDPxE1? NFnOWoEzPCCq NGPTdmxFVVOR M4HhWGlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gcZV1[W62IF\k[Yx1[TVyODDDSnRTKHC{b4TlbY4h[XCrY3HsJJN2em[jY3WgcI9k[WyrenH0bY9v MV:xPVk3Pjd6OR?=
Primary bronchial epithelial cell NISyZ4pMcW6jc3WgRZN{[Xl? MWWxJO69VQ>? MmLuPVYhcA>? MX3EUXNQ M2TwRmlvcGmkaYTpc44hd2ZiSFTBR|ch[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gd4hwenRiY3nyZ5VqfCCldYLy[Y51ew>? MoDlNVk6PjZ5OEm=
BxPC3 NF3wUVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHvdFR3PzJiaB?= NVHpfFFrUUN3ME2yMlch|ryP MXmyNFE1Ozd5OB?=
CAL27 NYPOWldZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX24NZNoPzJiaB?= MoT0TWM2OD1|LkeyJO69VQ>? MoDPNlAyPDN5N{i=
Capan1 MkDES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHZO|IhcA>? MUnJR|UxRTdwMzFOwG0> NXrmSFc6OjBzNEO3O|g>
HCC827 M1;aSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2n0SlczKGh? NX3QO4l2UUN3ME2xMlgh|ryP NIPkNoYzODF2M{e3PC=>
LNCAP MmL5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mm\DO|IhcA>? NXnUWoszUUN3ME2wMlU5KM7:TR?= M2TWc|IxOTR|N{e4
NCI-H2122 NYLZ[XBnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LVXlczKGh? M1jR[GlEPTB;Nz61JO69VQ>? MYGyNFE1Ozd5OB?=
NCI-H358 NVnWXWNXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3nGb|czKGh? M{izWWlEPTB;Mj61JO69VQ>? MoK5NlAyPDN5N{i=
SCC15 NXX0OI1VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\CU|czKGh? MWnJR|UxRTJwNEmg{txO M1fTNlIxOTR|N{e4
SCC25 Ml;RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXm0cHNYPzJiaB?= M1uyVmlEPTB;MT65N{DPxE1? NHTkfG8zODF2M{e3PC=>
SKHEP1 MkjCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojPO|IhcA>? NWW1RWVHUUN3ME2zMlQ3KM7:TR?= NVjKW4sxOjBzNEO3O|g>
BJ M1LMT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFr1SWgyODBizszN NYTrfIJpPDhiaB?= M3WxbWROW09? Mmq0S5Jwf3SqIHnubIljcXSrb36gZZQhOC5yNTD0c{AyODBidV2= NI\WWFkzODF2M{i0NC=>
MUF NYLzRph7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3P4PVExOCEQvF2= NITTPYk1QCCq NGXZO3VFVVOR MVLHdo94fGhiaX7obYJqfGmxbjDheEAxNjB3IITvJFExOCC3TR?= NWHNPW1vOjBzNEO4OFA>
Caco-2 NF\xOI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorCNVAxKM7:TR?= NEHJ[mY1QGh? NVrBblk5TE2VTx?= NH3mV4tKSzVyPUiuNUDPxE1? NX61eIJ6OjBzNEO4OFA>
Caco-2 NIrVSJRMcW6jc3WgRZN{[Xl? NH32fpM2KM7:TR?= MVy0JIg> M{nBSmROW09? NHy2eINKdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBpcXO2b37lJIFk\XS7bHH0bY9vKEmFNUCgc4YhOC5yM{eg{txO MofVNlAyPDN6NEC=
Caco-2 M{DJOGtqdmG|ZTDBd5NigQ>? MVSwMlMyPiCwTTD0c{A{OS54IN88US=> NUHBZXVtPCCq MlzYSG1UVw>? MUHFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzFid3n0bEBKSzVyIH;mJFAvOTF7IN88US=> NGPFXnUzODF2M{i0NC=>
Caco-2 M2e5fmtqdmG|ZTDBd5NigQ>? M36zNVAvOzF4IH7NJJRwKDNzLk[g{txO MnPGOEBp M1rtemROW09? NVj5eJh3TXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVOzJJdqfGhiSVO1NEBw\iByLkG0O{DPxE1? NXHkfXNXOjBzNEO4OFA>
Caco-2 MonuT4lv[XOnIFHzd4F6 NHH6W4I3NjN{IH7NJJRwKDZ|MjFOwG0> MY[0JIg> NXzDboFLTE2VTx?= MWLFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzVid3n0bEBKSzVyIH;mJFEzNjZizszN NWPMN3ZyOjBzNEO4OFA>
Caco-2 MW\LbY5ie2ViQYPzZZk> NFPMWFU3NjN{IH7NJJRwKDZ|MjFOwG0> NIHL[4M1KGh? NFPMNHVFVVOR MVvFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzdid3n0bEBKSzVyIH;mJFQyKM7:TR?= MYqyNFE1Ozh2MB?=
Caco-2 MUPLbY5ie2ViQYPzZZk> MWewMlMyPiCwTTD0c{A{OS54IN88US=> NXrGZY91PCCq NHPmN3dFVVOR NYDDW2lOTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO2 M1nUXlIxOTR|OESw
HepG2 M4PjO2tqdmG|ZTDBd5NigQ>? MlvwOUDPxE1? NEPOfmQ3cA>? M1LYZWROW09? MXrJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;u MVqyNFE1Ozh2MB?=
HepG2 MlHlT4lv[XOnIFHzd4F6 MVm1JO69VQ>? NVi2WY5pPmh? Mk\RSG1UVw>? M3vlSmlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgbJlx\XKjY3X0fYxifGmxbh?= NGD2[o8zODF2M{i0NC=>
HepG2 NXrkSpBIU2mwYYPlJGF{e2G7 MVu1JO69VQ>? M4P0fVZp NXKxenR{TE2VTx?= MWXJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDhcJBp[S22dXL1cIlvKGGlZYT5cIF1cW:w NIXPNHgzODF2M{i0NC=>
HepG2 NEHkWmpMcW6jc3WgRZN{[Xl? NFXHWHY2KM7:TR?= NUHX[FlJPmh? NUW2[YtXTE2VTx?= M2TxZWlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJGg{N0h2IHjpd5RwdmViYXPleJlt[XSrb36= M2TGeFIxOTR|OESw
COLO205 NGTKXVNMcW6jc3WgRZN{[Xl? MX2xNEDPxE1? MWiyOEBp MlfwSG1UVw>? NFj2ZlBKdmirYnn0bY9vKG:oIFjERWMyKGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBpcXO2b37lJGg{KGGlZYT5cIF1cW:wIIfpeIghUUN3MDDv[kAzNjJizszN NEXIeGUzODR3MUO3PC=>
SCC4 M{m5ZWtqdmG|ZTDBd5NigQ>? M1W0blEh|ryP NFXjfokzPGh? NGHjPGlKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6geJVjfWyrbjDhZ4V1gWyjdHnvci=> M{HFZ|IxPDV{MkK1
SCC4 MULLbY5ie2ViQYPzZZk> NV\XTnVOOSEQvF2= NXG5PXI2OjSq M{DaUWlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIHHj[ZR6dGG2aX;u NX7k[5ExOjB2NUKyNlU>
SCC4 MlrWSpVv[3Srb36gRZN{[Xl? NVjZVXZiOSEQvF2= MmfEOUBucW5? NEnvSWxC\2:waYP0JIFkfGm4aYT5JIF1KF[GUjDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiQ2nQNlQh\2WwZTDlfJBz\XO|aX;u MUCyNFQ2OjJ{NR?=
NB4 M4SxNGZ2dmO2aX;uJGF{e2G7 MXi1JO69VQ>? NGr6RlEzPCCq NV;uNVVpTE2VTx?= NXzpWnhiS2WubDDjfYNt\SCjcoLld5Qh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZYNkfW23bHH0bY9vKGG2IIDy[U1IOSCyaHHz[S=> M1[1e|IxPDlzNESw
NB4 MmPqT4lv[XOnIFHzd4F6 MorROUDPxE1? NYj5cIZ5OjRiaB?= MUnEUXNQ MXjJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iYXPleJlt[XSnIIT1ZpVtcW5ibHX2[Yw> NIfqfGczODR7MUS0NC=>
NB4 NFLIT4tHfW6ldHnvckBCe3OjeR?= MX21JO69VQ>? M{nRSFI1KGh? NYG1N2lzTE2VTx?= NFOwfJZKdmS3Y4Tpc44hd2ZicEKxW2FHOS:FSWCxJIV5eHKnc4Ppc44> M4\5VlIxPDlzNESw
NB4 Mlj1T4lv[XOnIFHzd4F6 M4nJUlUh|ryP NG\ZOWEzPCCq NVL6RXZHTE2VTx?= M1zjTmlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFizJIFk\XS7bHH0bY9v M3XMXFIxPDlzNESw
NUGC3 M4f1cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX2xR5NWT0l3ME2yMlk1KM7:TR?= M1zoO|IxQDVyOUex
A549 MW\GeY5kfGmxbjDBd5NigQ>? NWK4bZUyPSEQvF2= NXzhTGpXOyCq NXSwNVdqTE2VTx?= M2f6eGlv\HWldHnvckBw\iCjdYTvdIhi\3liaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCOQ{PJTS=> MWGyNFg2PTJyOB?=
HUT78 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17afFczKGh? NWjpT|VGTE2VTx?= M4TxTGdKPTB;MD6zJO69VQ>? NV;jN2xjOjB6OESyNFg>
U937 NUO5ZWZGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjMbVI4OiCq MYLEUXNQ NYLmUY9sT0l3ME2wMlM6KM7:TR?= NUX2bnhWOjB6OESyNFg>
A431 NE[zV3hEgXSxdH;4bYMhSXO|YYm= NESzTZc4OiCq NX3tSJV2S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTR|MTDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghUUN3MDDv[kA1NjJizszN M{fDTFIyODhyNkK5
A549 NXKxXno4S3m2b4TvfIlkKEG|c3H5 MVW3NkBp MWPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJI93\XKneIDy[ZN{cW6pIFjERWMhf2m2aDDJR|UxKG:oIEGuPEDPxE1? MkPiNlExQDB4Mkm=
CAL27 NYnlWlZWS3m2b4TvfIlkKEG|c3H5 NVPtfZpRPzJiaB?= NEDuO|dEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBESUx{NzDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghUUN3MDDv[kA{NjJizszN NEW0[mYzOTB6ME[yPS=>
HeLa NEPVUnBEgXSxdH;4bYMhSXO|YYm= Mm\QO|IhcA>? NWi2Zpo1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWOYTDj[YxteyCxdnXy[ZhxemW|c3nu[{BJTEGFIIfpeIghUUN3MDDv[kAyNjVizszN NUS1NZVsOjFyOEC2Nlk>
Sf21 NX64flJqU2mwYYPlJGF{e2G7 NIHrXmY{KGh? MWLJcohq[mm2aX;uJI9nKE[uYXegeIFo\2WmIHj1cYFvKHKnY3;tZolv[W62IFjERWM5KGW6cILld5Nm\CC5aYToJGlEPTBib3[gNU4zKM7:TR?= MoT6NlExQDB4Mkm=
SKBR3 NXLKfZh2S3m2b4TvfIlkKEG|c3H5 NWCydHU4PzJiaB?= NWTySGsxS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uEUkOgZ4VtdHNib4\ldoV5eHKnc4PpcochUEWUMjD3bZRpKEmFNUCgc4YhOi54IN88US=> MYqyNVA5ODZ{OR?=
SKOV3 Mlr1R5l1d3SxeHnjJGF{e2G7 MmjGO|IhcA>? MUTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISXIzKHerdHigTWM2OCCxZjCyMlIh|ryP MnrqNlExQDB4Mkm=
COR-L23 NYT6OGU3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHOyc5AyOCEQvF2= NYPiZ4RjPzJiaB?= NV;KSnluTE2VTx?= M4HNN2dKPTB;MT6xJO69VQ>? M3rkR|IyODhyNkS3
DU145 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGLlbHgyOCEQvF2= M2jwfFczKGh? M4nzeGROW09? MWnHTVUxRTFwMEWg{txO NYX2c4ZEOjFyOEC2OFc>
LoVo NVfJSVhXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjENVAh|ryP NF7JU5c4OiCq NUHjOHRRTE2VTx?= MWjHTVUxRTFwNzFOwG0> MYiyNVA5ODZ2Nx?=
MCR5-SV2 NIXndoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlS3NVAh|ryP NWX0UZllPzJiaB?= NYHDcZQ{TE2VTx?= MkC4S2k2OD1{LkGg{txO NYG3SGRNOjFyOEC2OFc>
HCT116 NFPm[5pMcW6jc3WgRZN{[Xl? NEK1Nmk{OCEQvF2= M3jmSVMxKGh? M1y1b2ROW09? NIfKOmNKdmirYnn0bY9vKG:oIFjERWM3KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBidHCqYT30eYJ2dGmwIHHj[ZR6dGG2aX;u NWq5fFVGOjFyOEC2OFc>
HCT116 M{ezUWtqdmG|ZTDBd5NigQ>? M{DzR|MxKM7:TR?= M3HNbFMxKGh? NXPoUYZITE2VTx?= NYLIdWxZUW6qaXLpeIlwdiCxZjDISGFEOSCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhcGm|dH;u[UBJO0t7IHHj[ZR6dGG2aX;u NG\DTJczOTB6ME[0Oy=>
U937 MmSzT4lv[XOnIFHzd4F6 MVG1JO69VQ>? MV[yOEBp MlzUSG1UVw>? M2TGNGlvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDv[kBi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KEt7 MlzPNlEzQTJ2OUK=
U937 M1PUbmtqdmG|ZTDBd5NigQ>? M2[0WVUh|ryP NFjPdmMzPCCq MnzpSG1UVw>? M3;iOWlvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDv[kBi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KEtzOB?= Ml2xNlEzQTJ2OUK=
U937 NXix[lRtU2mwYYPlJGF{e2G7 Mn\4OUDPxE1? MoOyNlQhcA>? M1mzUWROW09? MkHpTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6|ZnXyZZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJJBidi2jY3X0fYxifGWmIHjpd5RwdmViSESgcIV3\Wx? M3T0dFIyOjl{NEmy
U937 NUjzNVdPU2mwYYPlJGF{e2G7 MoHOOUDPxE1? NIXU[40yPSCvaX6= NYrXR4hzTE2VTx?= M2W1ZmlvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDv[kBpcXO2b37lJGg{KGGlZYT5cIF1cW:wIHzleoVt NVS5coV1OjF{OUK0PVI>
A2780 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\KSFk3KGh? MnnxSG1UVw>? NUnyVlZnUUN3ME2xMlYzKM7:TR?= NYrU[3JoOjF4M{S0N|A>
COLO205 MkXsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXy5OkBp M4PCXmROW09? MXXJR|UxRTJwMUKg{txO NW\scIJJOjF4M{S0N|A>
Hep3B MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUK0PEBp NE\HcopFVVOR NVfNOZFnT0l3ME2xMlIyKM7:TR?= MoDyNlE4OTJzNE[=
GM11272 NVjOUFdTTnWwY4Tpc44hSXO|YYm= MnvoNVAxKG6P MlO4O|IhcA>? NUjufFVnTE2VTx?= NU[4cXljWmWjY4TpeoF1cW:wIH;mJG1mS3B{IH31eIFvfCCneIDy[ZN{cW:wIHH0JFExKHSxIEGwNEBvVQ>? MXSyNVg1ODdzNh?=
CFBE41o M2LINGtqdmG|ZTDBd5NigQ>? M4G5dVUh|ryP M4naN2ROW09? MX7Ec5dvKHKnZ4XsZZRqd25ib3[gTGRCSzdiYYSgNE4zKHSxIEWgeW0> NF\ibHUzOTl6NEm1PC=>
CFBE41o MWrGeY5kfGmxbjDBd5NigQ>? NWnIO4ttOSEQvF2= MUCyOEBp NWHEcYJpTE2VTx?= MXHS[ZN1d3KjdHnvckBw\iCFRmTSJIRmdHSjRkWwPEBufXSjboSgeJJi\m[rY3vpcochfG9iY3XscEB{fXKoYXPlJIF{KGeueXPv[o9zdXN? NVHKPHZpOjF7OES5OVg>
DU145 M1TXfmtqdmG|ZTDBd5NigQ>? M33DVlIvPSEQvF2= NFjGVZozPCCq NVjtc3JSTE2VTx?= NIXlRlhKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNidYDy[Yd2dGG2aX;uJI9nKHB{MYfh[lEheHKxdHXpckBmgHC{ZYPzbY9v M4\PTFIzOjZyMU[2
DU145 NFT2Z5ZMcW6jc3WgRZN{[Xl? MXi1JO69VQ>? MkX6NlQhcA>? Ml3ISG1UVw>? NUftZWxSUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHjpd5RwdmViSESgZYNmfHmuYYTpc44> NVjUU3U5OjJ{NkCxOlY>
DU145 MmXJT4lv[XOnIFHzd4F6 NF;3ToI2KM7:TR?= NUC1V2d[OjRiaB?= MV\EUXNQ NWjtPXBDUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHjpd5RwdmViSEOgbJlx\XKjY3X0fYxifGmxbh?= NYf0S49jOjJ{NkCxOlY>
786-0 NVrZbXVOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWK2MlIh|ryP NUDETVdzPzJiaB?= MWnEUXNQ M2PGVmdKPTB;Mz6yJO69VQ>? NYHTXYtKOjJ2M{W2Olk>
A498 NX7w[YRnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLJOYVJPi5{IN88US=> NY\ZSYtTPzJiaB?= M1P6V2ROW09? MnTRS2k2OD1zLk[g{txO MXmyNlQ{PTZ4OR?=
A549/ATCC M13wZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHWzPIY3NjJizszN MlTwO|IhcA>? NH;ZbY1FVVOR Mor2S2k2OD1zLk[g{txO MlfVNlI1OzV4Nkm=
ACHN NWGyOIVvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrpZoxsPi5{IN88US=> NVXUSGZMPzJiaB?= MkfOSG1UVw>? NHvHd4ZIUTVyPUGuN{DPxE1? NEDwPXczOjR|NU[2PS=>
BT549 NWjw[VFoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4\UbFYvOiEQvF2= NIX3fZo4OiCq NEC5ZXJFVVOR NIDqeZhIUTVyPUGuN{DPxE1? NE\FUY4zOjR|NU[2PS=>
EKVX M33sd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;NO2pEPi5{IN88US=> NVTHcYo2PzJiaB?= MW\EUXNQ NXvBNVc5T0l3ME2xMlMh|ryP M3:4NVIzPDN3Nk[5
HCT15 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfQcGtsPi5{IN88US=> MXO3NkBp M3izS2ROW09? NYnhWWQ6T0l3ME2yMlUh|ryP NU\1UGN[OjJ2M{W2Olk>
HOP92 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXm2MlIh|ryP MX:3NkBp M1rIOmROW09? MXPHTVUxRTRizszN Mk\NNlI1OzV4Nkm=
IGROV1 NEC4T|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWn0V|BiPi5{IN88US=> MoPJO|IhcA>? M2HESmROW09? NFfuPWdIUTVyPUGuN{DPxE1? NEPEeYYzOjR|NU[2PS=>
LOXIMVI MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUe2MlIh|ryP M{\NS|czKGh? NH\tUGpFVVOR MmXYS2k2OD1zIN88US=> NIf0dYkzOjR|NU[2PS=>
M14 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYq2MlIh|ryP MYi3NkBp M{Xh[GROW09? MWDHTVUxRTFwMzFOwG0> MkfiNlI1OzV4Nkm=
MALME-3M NWfsNWQ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLiNmc6Pi5{IN88US=> NYr0RZd1PzJiaB?= NFWzeIlFVVOR NHvTNYxIUTVyPUCuN|Ih|ryP NH\1TVczOjR|NU[2PS=>
MCF10A NVTKSpFxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PPflYvOiEQvF2= MVy0PEBp M2HSdGROW09? MU\HTVUxRTlwNjFOwG0> M2TNelIzPDN3Nk[5
MDA-MB-435 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDpOk4zKM7:TR?= M{m0fVczKGh? M4SzcmROW09? NVnXR4d2T0l3ME2wMlUh|ryP MkHrNlI1OzV4Nkm=
MDA-MB-468 NUjEeVFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYm2MlIh|ryP NWTn[WJkPzJiaB?= NH3DV|RFVVOR NG\n[nlIUTVyPUCuOlMh|ryP NGnJfFQzOjR|NU[2PS=>
MOLT4 Mof2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HHelYvOiEQvF2= M3ixdVczKGh? M4[zZmROW09? NI\VW|RIUTVyPUCuOEDPxE1? MnfUNlI1OzV4Nkm=
NCI-ADR-RES M2DaZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfGWHlUPi5{IN88US=> MUK3NkBp MWPEUXNQ MlXmS2k2OD1yLkG2JO69VQ>? M4j1XlIzPDN3Nk[5
NCI-H23 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1HicFYvOiEQvF2= M4T5TlczKGh? MXfEUXNQ NYC2OpRxT0l3ME2xJO69VQ>? NF7sflAzOjR|NU[2PS=>
NCI-H322M NFm4cGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljoOk4zKM7:TR?= NGHVeHU4OiCq MlzySG1UVw>? M{e3TmdKPTB;MD63PUDPxE1? NVTIU25COjJ2M{W2Olk>
NCI-H460 NIPpWJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\PNIk3NjJizszN Ml\PO|IhcA>? NFLxb3FFVVOR M1qwb2dKPTB;MD63PUDPxE1? M{jsV|IzPDN3Nk[5
NCI-H522 NIiyUGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2WycFYvOiEQvF2= MomwO|IhcA>? MV7EUXNQ NI\5ZXBIUTVyPUCuOUDPxE1? NFXOUnMzOjR|NU[2PS=>
OVCAR3 NFzWZYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWf3U5BLPi5{IN88US=> MorwO|IhcA>? M4HndGROW09? M4L5PWdKPTB;MT6zJO69VQ>? Mlm0NlI1OzV4Nkm=
OVCAR4 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjqOk4zKM7:TR?= NUD3codVPzJiaB?= MYXEUXNQ NFj0RY5IUTVyPUSg{txO M1LYZlIzPDN3Nk[5
OVCAR5 NVrtPWl6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXm2MlIh|ryP MXq3NkBp NITDeY9FVVOR NHG2PIlIUTVyPUCuO|kh|ryP NEfmUnIzOjR|NU[2PS=>
OVCAR8 NYTTV|BTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnmNXdJPi5{IN88US=> NHjISJY4OiCq NHmzc|RFVVOR NYnq[WY6T0l3ME2wMlUh|ryP NV\UU20xOjJ2M{W2Olk>
RXF393 NGPVZ41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIr2NXI3NjJizszN MkS3O|IhcA>? MXvEUXNQ NHuz[mNIUTVyPUGuN{DPxE1? MlfHNlI1OzV4Nkm=
SF268 NVPrXWlVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7ENFQ3NjJizszN M1vReVczKGh? MWrEUXNQ M2\3dWdKPTB;MT62JO69VQ>? NFyxVZIzOjR|NU[2PS=>
SF295 NX7ub3M{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnn5Ok4zKM7:TR?= Moj1O|IhcA>? M4fmWGROW09? M{\XbmdKPTB;MT62JO69VQ>? NWDBVopjOjJ2M{W2Olk>
SF539 M2LLUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrpOk4zKM7:TR?= NUWzSGVjPzJiaB?= M3TPc2ROW09? M4fjWmdKPTB;MjFOwG0> MnjuNlI1OzV4Nkm=
SK-MEL-2 NWnpfVN7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYS2MlIh|ryP NHr3XnE4OiCq NHXTOnFFVVOR MoDBS2k2OD1zLkOg{txO M3HhPVIzPDN3Nk[5
SK-MEL-28 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvjbog3NjJizszN NH3ZR3Y4OiCq MW\EUXNQ MkXHS2k2OD1zIN88US=> Mn3TNlI1OzV4Nkm=
SK-MEL-5 NH36WJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWS2MlIh|ryP NHn3VZo4OiCq NWTae2pkTE2VTx?= NYP3SY5kT0l3ME2wMlY{KM7:TR?= M2[3blIzPDN3Nk[5
SKOV3 M3z4Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHJPVVNPi5{IN88US=> MWS3NkBp NXvCSVh[TE2VTx?= NHfIZ|lIUTVyPUGg{txO M3XzOlIzPDN3Nk[5
SN12C M1XoVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWe2c5VxPi5{IN88US=> NUHmNGVzPzJiaB?= NHW1NnhFVVOR NXKzXHRKT0l3ME2yJO69VQ>? MWqyNlQ{PTZ4OR?=
SNB19 NX;BWZRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUK2MlIh|ryP MW[3NkBp NFTMdHpFVVOR MlvvS2k2OD1yLk[zJO69VQ>? MYiyNlQ{PTZ4OR?=
SNB75 NUjEOYk5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rsZVYvOiEQvF2= M162dFczKGh? MlHvSG1UVw>? MWfHTVUxRTBwN{mg{txO NGrTOFczOjR|NU[2PS=>
SR MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MU[2MlIh|ryP NFnBcnE4OiCq NV7IT4xQTE2VTx?= NVfCPVFUT0l3ME2wMlQh|ryP NF3hS3YzOjR|NU[2PS=>
T47D NF;kSpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3PXZI3NjJizszN MXe3NkBp MkjUSG1UVw>? M4f5e2dKPTB;MD61JO69VQ>? Mlq3NlI1OzV4Nkm=
TK10 MmrKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml3oOk4zKM7:TR?= NHTYU3I4OiCq M1:4ZWROW09? NIDobZZIUTVyPUCuOlMh|ryP NXL4XlYxOjJ2M{W2Olk>
UACC257 NXH1UHQ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1qxNlYvOiEQvF2= MVW3NkBp NHjybJVFVVOR MVLHTVUxRTBwNTFOwG0> NXTSeotoOjJ2M{W2Olk>
UACC62 NI\jRo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml[zOk4zKM7:TR?= NF;Me404OiCq Mlr0SG1UVw>? M4GxRmdKPTB;MD60JO69VQ>? MojZNlI1OzV4Nkm=
UO31 MkDPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUG2MlIh|ryP M3TOUlczKGh? M3n2[WROW09? NXzHZVJOT0l3ME2wMlUh|ryP MUeyNlQ{PTZ4OR?=
Hep3B NH32WI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3HaU|Q5KGh? M{[1SmROW09? NEPYXlZIUTVyPUCuOlkh|ryP M{jDcFIzPDN7OE[z
BE(2)-C M1OxcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUK3NkBp MlHoSG1UVw>? MoPzTWM4OD1{IN88US=> NVPYem9COjJ7M{KzNVY>
Bel7402 NGj1SY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\qN|czKGh? Mn3GSG1UVw>? Mn[1S2k6OD1zNzFOwG0> NIPmTpkzOzB4MUO3Oi=>
Bel7404 M1LERWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\KN5FFPzJiaB?= M1rSWmROW09? M3:0NGdKQTB;MUig{txO M3ThcVI{ODZzM{e2
BGC823 NWTQVFRzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rPc|czKGh? MXfEUXNQ NGPEXVlIUTlyPUeuOUDPxE1? MXWyN|A3OTN5Nh?=
MGC803 NEfWSm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPKO|IhcA>? M1PGUWROW09? NFLtPXJIUTlyPUGwMlgh|ryP MYWyN|A3OTN5Nh?=
SGC7901 MmTES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVm3NkBp M3fnWGROW09? NXzDOVdFT0l7ME25MlQh|ryP NGXXdI4zOzB4MUO3Oi=>
SKHEP1 Mn\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXaO|IhcA>? M4XNSGROW09? MYDHTVkxRTF5IN88US=> M1zw[VI{ODZzM{e2
SMMC7721 NWfPfXhiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPVO|IhcA>? MlXzSG1UVw>? MWnHTVkxRTJyIN88US=> MVSyN|A3OTN5Nh?=
NUGC3 M4rOW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn70NVAh|ryP NX30UGZpT0l;Mj65OEDPxE1? MoG3NlMyPjN|M{K=
A2780 CisR MXLLbY5ie2ViQYPzZZk> NWC2UItYOThiaB?= MXzJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNESg{txO MVKyN|I2OjZyMx?=
CAL27 sens NEi1Z4ZMcW6jc3WgRZN{[Xl? Moj0NVghcA>? M{e4VmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD6zOkDPxE1? MnfBNlMzPTJ4MEO=
CAL27 CisR M3vPO2tqdmG|ZTDBd5NigQ>? MoDUNVghcA>? NEPnUHFKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOlEh|ryP MnvpNlMzPTJ4MEO=
KYSE-510 CisR MWTLbY5ie2ViQYPzZZk> MVmxPEBp MX3Jcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNUmg{txO NXvScoN3OjN{NUK2NFM>
KYSE-510 sens NV;2RmZCU2mwYYPlJGF{e2G7 M134ZVE5KGh? M1nT[2lvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD63JO69VQ>? NHT4XFMzOzJ3Mk[wNy=>
MDA-MB-231 sens MXzLbY5ie2ViQYPzZZk> NUHWdXRWOThiaB?= M37ROmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD62NUDPxE1? NFrTT4IzOzJ3Mk[wNy=>
MDA-MB-231 CisR NFzi[nlMcW6jc3WgRZN{[Xl? MVWxPEBp M17QcGlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD62NUDPxE1? NWq2fGtDOjN{NUK2NFM>
ES2 M4fadGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYi0PEBp NFHoO2hFVVOR MnP4TWM2OD1zMj63JO69VQ>? NI\uR28zOzR7M{S0PS=>
MDA-MB-231 MVXLbY5ie2ViQYPzZZk> NGLRelQyOCEQvF2= MnLFN|AhdWmw MXHEUXNQ M2X0NmlvcGmkaYTpc44hd2ZiSFTBR|Ji NGDLR20zOzR7M{S0PS=>
MDA-MB-231 NIDXS3lMcW6jc3WgRZN{[Xl? NFjSSFQyKM7:TR?= NHrMfYYzPCCq MnPsSG1UVw>? MVHJcohq[mm2aX;uJI9nKEiGQVO2JIF{e2W|c3XkJIF{KGGlZYT5cIF1cW:wIHzleoVteyCxZjD0eYJ2dGmw M4HidFI{PDl|NES5
HS68 MYLDfZRwfG:6aXOgRZN{[Xl? MXWxNEDPxE1? MUC3NkBp M37Cb2ROW09? M3PMN2lEPTB;ND62JO69VQ>? NXXMTnVjOjN3N{C1OFI>
MEF MnfXR5l1d3SxeHnjJGF{e2G7 M{C1fFExKM7:TR?= MmLuO|IhcA>? MnXkSG1UVw>? MVrJR|UxRTRwNDFOwG0> MXGyN|U4ODV2Mh?=
BGC823 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\MUoQ5TE2VTx?= NE\VNXVIUTVyPUOuNFkh|ryP M3PMfVI{PjBzN{C2
High5 NX3aPZFCU2mwYYPlJGF{e2G7 NIjI[IszPCCq MV7Jcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO2JJdqfGhiSVO1NEBw\iByLkC5JO69VQ>? MmTaNlM3ODJ3MkO=
High5 Ml\JT4lv[XOnIFHzd4F6 NHjwUpQzPCCq MVLJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOxJJdqfGhiSVO1NEBw\iByLkGyJO69VQ>? MmTMNlM3ODJ3MkO=
High5 M{PVTWtqdmG|ZTDBd5NigQ>? MoThNlQhcA>? M{PGUmlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR|Mhf2m2aDDJR|UxKG:oIECuNVch|ryP NEnK[FkzOzZyMkWyNy=>
A549 NFHCNWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFX5XIZFVVOR MVvJR|UxRTFwMEe5JO69VQ>? NHL5eHMzOzZ2NEKxNC=>
HCT116 NYj5VXlmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HpO2ROW09? MWrJR|UxRTBwN{W3JO69VQ>? NUXDZ2ZROjN4NESyNVA>
K562 MnXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXwclg6TE2VTx?= NH3sT4xKSzVyPUCuOlQ2KM7:TR?= MkLqNlM3PDR{MUC=
GBC-SD Mo\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml:wOUDPxE1? NEfaSW44OiCq NFL4bIRFVVOR NHTSU|hKSzVyPUG0Mlgh|ryP NFPhV|gzOzdyN{K2NC=>
HGC27 NVLFfpY{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIXmPFM2KM7:TR?= MWG3NkBp MUHEUXNQ MnzNTWM2OD13LkSg{txO NUDIWVF3OjN5MEeyOlA>
HL60 M4fHRWtqdmG|ZTDBd5NigQ>? MWm1JO69VQ>? MXeyOEBp NXzkOphMTE2VTx?= MoHZTY5pcWKrdHnvckBw\iCKRFHD MmnGNlM4ODd{NkC=
Vero MmnNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWO4NFd3PzJiaB?= Mo\ESG1UVw>? NYO2TVFsUUN3ME2wMlkh|ryP NHzjUHYzOzd6NkS1Ni=>
NCI60 MWnDfZRwfG:6aXOgRZN{[Xl? NYjibo5IPDhiaB?= MnfqSG1UVw>? NUSy[VczT0l;MD61N{DPxE1? MmnWNlQxOTV|Mke=
SW620 MkL1R5l1d3SxeHnjJGF{e2G7 MV20PEBp M4LUd2ROW09? MXnHTV0xNjV2IN88US=> Mle2NlQxOTV|Mke=
U251 Mli3R5l1d3SxeHnjJGF{e2G7 MkDSOFghcA>? NGjLbXhFVVOR NWDXRo1qT0l;MT61N{DPxE1? MWWyOFAyPTN{Nx?=
KM12 NInIXmJEgXSxdH;4bYMhSXO|YYm= MVe0PEBp M1rITmROW09? MkXUS2k:OS56ODFOwG0> MnG2NlQxOTV|Mke=
Hs578T MYjDfZRwfG:6aXOgRZN{[Xl? M17DWFQ5KGh? NVizeXVDTE2VTx?= M13uemdKRTRwOEOg{txO MW[yOFAyPTN{Nx?=
MDA-MB-231 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF7qd|I1QCCq MlLSTWM2OD1yLkG2JO69VQ>? M2\5dlI1ODl3MEG2
MCF7 NX;BU2h2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLXOFghcA>? M3;1TWdKRTBwNEWg{txO MnPnNlQyOTl3NUW=
MDA-MB-435 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXfiWlAzPDhiaB?= NID6eWVIUT1yLki4JO69VQ>? MkHnNlQyOTl3NUW=
AsPC1 NUDnTVVzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmjQN|Ah|ryP M1rjSFQ5KGh? NFnTbW5FVVOR MU\JR|UxRTNwNk[g{txO NXnnRVdOOjRzOEWzO|g>
Jurkat E6.1 MV;LbY5ie2ViQYPzZZk> MoG1NkBp NF;lXWdFVVOR MXHJcohq[mm2aX;uJI9nKEiGQVO0JJdqfGhiSVO1NEBw\iByLkC0JO69VQ>? NFX5Nm4zPDJ4MUi2Ni=>
KG1 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjuOFghcA>? NH;FZohKSzVyPUGuNVUh|ryP MknQNlQ2OjVyMEO=
HEL NVm4ZnhpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWC0PEBp MV7EUXNQ M2jpUWlEPTB;MD60PUDPxE1? M3:2V|I1Pjl2MEW1
HSC3 MonjR5l1d3SxeHnjJGF{e2G7 NX3qdWg4OTJiaB?= MWjEUXNQ MlvsTWM2OD1yLkexJO69VQ>? M{LwcFI1PzZ4NU[w
HT-29 MnftR5l1d3SxeHnjJGF{e2G7 M{\lTFEzKGh? NEjQcppFVVOR MYDJR|UxRTBwN{Kg{txO MnLmNlQ4PjZ3NkC=
KB MlPFR5l1d3SxeHnjJGF{e2G7 MVuxNkBp MoHQSG1UVw>? NVOzUmpSUUN3ME2wMlc2KM7:TR?= M3XjO|I1PzZ4NU[w
H460 M4rWZWN6fG:2b4jpZ{BCe3OjeR?= NGrZdJoyOiCq MXjEUXNQ M{\UbWlEPTB;MD64NkDPxE1? Mk\tNlQ4PjZ3NkC=
MONE1 M1z5VGN6fG:2b4jpZ{BCe3OjeR?= NH73[XUyOiCq M1TwSWROW09? MWPJR|UxRTFwMUig{txO NW\VbVBPOjR5Nk[1OlA>
MKN45 NV7WUHozS3m2b4TvfIlkKEG|c3H5 M17HdlEzKGh? MVHEUXNQ NXLJ[pE6UUN3ME2xMlI2KM7:TR?= MknFNlQ4PjZ3NkC=
TSGH MkT4R5l1d3SxeHnjJGF{e2G7 MoDKNVIhcA>? NWO0OG1kTE2VTx?= MXrJR|UxRTJwMEmg{txO NIrjelczPDd4NkW2NC=>
HL60 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWW3NkBp NXuyO5RqTE2VTx?= M3PQc2dKPTB;MD65N{DPxE1? MYWyOFkxODVyMB?=
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RPMI-8226 NEPHcIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3LTWM2OD14N{iuNVghdk1? MV3TRW5ITVJ?
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NCI-H1155 M2G4d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGr1S3RKSzVyPUKuN|Y4OjVizszN MUTTRW5ITVJ?
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NCI-H650 MnTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV76bItPUUN3ME2zMlY1Ojh5IN88US=> M3zx[3NCVkeHUh?=
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CAL-12T MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTRwMUOxNFMh|ryP NUCwNGNpW0GQR1XS
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T98G MmjuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVTPS5k6UUN3ME20MlIzOTV5IN88US=> MmfFV2FPT0WU
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KLE Ml7ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTlwM{KyN|Ih|ryP Mlz2V2FPT0WU
Calu-6 M1jZe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXNTWM2OD17LkO3OVI5KM7:TR?= NEDoSHlUSU6JRWK=
LB831-BLC MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\NTWM2OD17LkS1NVcyKM7:TR?= NVPUSFlSW0GQR1XS
SW684 M1;qU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\MSphKSzVyPUmuOFYyOTZizszN M3;hV3NCVkeHUh?=
SH-4 M2HldGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTlwNEmzOlQh|ryP NV\WSW93W0GQR1XS
IST-MES1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M120NmlEPTB;OT61NVY1PCEQvF2= M1;TNnNCVkeHUh?=
Saos-2 NWrKbFROT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTNTWM2OD17LkWyNVk2KM7:TR?= NH7qNmFUSU6JRWK=
NCI-H2087 NWrI[VhWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLkR3R[UUN3ME25MlY4OzV{IN88US=> NFXZUXdUSU6JRWK=
SCC-9 M4H1bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTlwN{G4NVYh|ryP NVnuTJhmW0GQR1XS
MFE-280 MmLaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jsV2lEPTB;OT63Nlc5PSEQvF2= MoTyV2FPT0WU
BEN MoHVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTsVmYzUUN3ME25MlgzOTV7IN88US=> Mo\GV2FPT0WU
CP50-MEL-B NHjD[HRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvETWM2OD17LkmyOFMzKM7:TR?= MXnTRW5ITVJ?
SK-MEL-28 NH76NJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvHcWN2UUN3ME2xNE4xOTl|IN88US=> M{fLO3NCVkeHUh?=
NCI-H2452 NUn3ZlZGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUT3TYxwUUN3ME2xNE4yPiEQvF2= MUnTRW5ITVJ?
NCI-H1395 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGW5fldKSzVyPUGwMlE4QCEQvF2= MV;TRW5ITVJ?
NCI-H2052 NF7LZpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\uO|RxUUN3ME2xNE4yQDh{IN88US=> M3fad3NCVkeHUh?=
A375 NVfW[G1YT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnyyTWM2OD1zMD6yOFUzKM7:TR?= NX7qSlc2W0GQR1XS
HCC38 NWfCeHlsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmflTWM2OD1zMD6zNlIh|ryP MWTTRW5ITVJ?
LK-2 MmXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;pZmlEPTB;MUCuOFIxQCEQvF2= MW\TRW5ITVJ?
SF126 MlSwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrHc|BqUUN3ME2xNE41PzJ5IN88US=> NUPJbXBRW0GQR1XS
SK-MEL-1 NYLlflhvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PLOGlEPTB;MUCuO|g{QCEQvF2= MV;TRW5ITVJ?
ACN M3zoTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3vSN2lEPTB;MUCuPFY4QSEQvF2= NI\rR4RUSU6JRWK=
BB30-HNC NFHRZmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTFyLki3NVEh|ryP MWTTRW5ITVJ?
NCI-H747 M3zaWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MonpTWM2OD1zMD64O|Q3KM7:TR?= M4naWXNCVkeHUh?=
KYSE-150 MlPYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXsTWM2OD1zMT6wN|g4KM7:TR?= M2[4SnNCVkeHUh?=
D-247MG NFXmWWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rBZWlEPTB;MUGuNVAxQSEQvF2= NWn0RpNiW0GQR1XS
BxPC-3 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDsfpRKSzVyPUGxMlM4PDdizszN NUnKV2VUW0GQR1XS
SK-MEL-30 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLHSpM1UUN3ME2xNU41ODd7IN88US=> MX7TRW5ITVJ?
CAS-1 NHXR[ZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkD6TWM2OD1zMT60NVA6KM7:TR?= NYfYWoNZW0GQR1XS
no-10 NHnOWYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jPb2lEPTB;MUKuNVIyPyEQvF2= NUDFS4E{W0GQR1XS
NCI-H2405 NG\pPWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTF{LkKwN|Yh|ryP M3;zfnNCVkeHUh?=
KGN NGrvZ4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3vTWM2OD1zMj6yPVAyKM7:TR?= MWTTRW5ITVJ?
BB49-HNC MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTrTWM2OD1zMj6zPVU3KM7:TR?= NIO1OXJUSU6JRWK=
NCI-H1693 NGLvdnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NED1RopKSzVyPUGyMlU5OTZizszN NXr2PY5sW0GQR1XS
RO82-W-1 NUT2TJY6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4ThRWlEPTB;MUOuNFY1QSEQvF2= NHjNSndUSU6JRWK=
CAL-85-1 M{fGSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDvem5KSzVyPUGzMlQ{QTlizszN MX\TRW5ITVJ?
H-EMC-SS MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU[wfWZwUUN3ME2xN{46OTB2IN88US=> NFrN[lhUSU6JRWK=
SW1783 NV3hdXFjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TYd2lEPTB;MUSuNlM6OSEQvF2= MlywV2FPT0WU
LB771-HNC M{HvTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXj5W2tqUUN3ME2xOE41OTlizszN NYPaTHl[W0GQR1XS
HCC70 NUP2VlZPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVjQeotYUUN3ME2xOE41OjN|IN88US=> NVjDcIdEW0GQR1XS
KNS-81-FD MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlH4TWM2OD1zND63NFQ2KM7:TR?= NY\NepA1W0GQR1XS
KYSE-520 NHLJ[HpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;ITWM2OD1zND65Nlch|ryP M3XJTHNCVkeHUh?=
TGBC24TKB NUH3emtNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV3W[YdOUUN3ME2xOE46PTN7IN88US=> MW\TRW5ITVJ?
SNB75 M{CyOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlG2TWM2OD1zNT6wO|I6KM7:TR?= M{\nO3NCVkeHUh?=
SW872 MoDPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIK2WVFKSzVyPUG1MlIzOThizszN MnLuV2FPT0WU
U031 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTF3LkiwNlgh|ryP NVzTfY82W0GQR1XS
ChaGo-K-1 MnG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTQTWM2OD1zNT64N|U6KM7:TR?= MUPTRW5ITVJ?
NCI-H441 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEjWR2JKSzVyPUG2MlAxOzlizszN NVz0WIFiW0GQR1XS
TE-12 MoX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHEc2RKSzVyPUG2MlE5PzNizszN MnHBV2FPT0WU
NCI-H1755 M3vtdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnj2TWM2OD1zNj61O|Mh|ryP MoXuV2FPT0WU
KNS-42 NF;UT2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXLJR|UxRTF4Lk[1PFkh|ryP NIfVRVRUSU6JRWK=
SK-PN-DW MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRTF5LkSyNFEh|ryP M2jGT3NCVkeHUh?=
NCI-H226 M3XrRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfKTWM2OD1zNz65N|Q2KM7:TR?= NW\WPYJIW0GQR1XS
K-562 M3;nZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NY\Ed49sUUN3ME2xPE43OzF5IN88US=> NHjqUJlUSU6JRWK=
A388 NHizb5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXu2e|F3UUN3ME2xPU4xOzB|IN88US=> NV76[pBrW0GQR1XS
ZR-75-30 NYHOSHpCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHmTWM2OD1zOT6wPVY5KM7:TR?= NFHKd2hUSU6JRWK=
LU-135 NICxW5dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvOTWM2OD1zOT6yOlM3KM7:TR?= M3P2XnNCVkeHUh?=
NCI-H2029 M1i1Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTF7Lk[wNlEh|ryP NGCzOpZUSU6JRWK=
HT-1197 NHnkXWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NED1emFKSzVyPUG5MlkzOjdizszN NVr6Tnp{W0GQR1XS
IGR-1 NWXhbWhXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlP1TWM2OD1{MD6zNVE4KM7:TR?= NIn3NHZUSU6JRWK=
U-87-MG M{XjZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVrNOGtOUUN3ME2yNE43PDF|IN88US=> MWTTRW5ITVJ?
NCI-H1304 NWmyWnVmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknNTWM2OD1{MD63OVAyKM7:TR?= NVHwXZBqW0GQR1XS
RCC10RGB M1nXXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH:4R5hKSzVyPUKxMlI5OjlizszN NYDVeJN[W0GQR1XS
NCI-H520 MnXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTJ{Lke2NVEh|ryP MYnTRW5ITVJ?
SK-LU-1 NFvoUGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFL2N4VKSzVyPUKzMlA4OThizszN MYrTRW5ITVJ?
UACC-893 MmDZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnEcI5KSzVyPUKzMlExOzhizszN MYXTRW5ITVJ?
NCI-H2347 MkTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUDJR|UxRTJ|LkK2O|Qh|ryP MnW2V2FPT0WU
U-118-MG Mnn0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnzPI9KSzVyPUKzMlczOTdizszN MmL4V2FPT0WU
NCI-H596 NITaTGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXETWM2OD1{Mz65NFU2KM7:TR?= NUTMO5dOW0GQR1XS
MZ1-PC NWHGWnJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmq4TWM2OD1{ND62O|U6KM7:TR?= NFO2dm9USU6JRWK=
SW1417 M3foc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV36VGN7UUN3ME2yOU4zPDl6IN88US=> NV\QcJltW0GQR1XS
NCI-H82 NGfoeWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;Xdmh2UUN3ME2yOk41Ojh6IN88US=> NXPBTY5oW0GQR1XS
COLO-678 M4PwXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULQfXIyUUN3ME2yO{41QTF7IN88US=> NGnxVHpUSU6JRWK=
KURAMOCHI MojjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;jSJNKSzVyPUK4MlEzPiEQvF2= NEn2dWlUSU6JRWK=
NCI-H1650 NFy0eHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHLS3pyUUN3ME2zNE4yQTJ2IN88US=> MWfTRW5ITVJ?
HCC1954 NWPiPIl6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4PLfGlEPTB;M{GuN|Y3PiEQvF2= NYXBdXhSW0GQR1XS
GAK NILjPY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4XHeGlEPTB;M{KuNlYyPiEQvF2= MlHjV2FPT0WU
YAPC MnXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrCTWM2OD1|Mz63OlQ5KM7:TR?= M4f6fXNCVkeHUh?=
NCI-H2228 NVX6SWtST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTN2LkO2NFUh|ryP M{\E[XNCVkeHUh?=
VMRC-RCZ NWD6fmY5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInQZ3RKSzVyPUO2MlMxOjJizszN MXrTRW5ITVJ?
NMC-G1 M{DjXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nMdmlEPTB;M{euNFUzQSEQvF2= M3\LfnNCVkeHUh?=
D-336MG MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\6bGZoUUN3ME20NU45PDR{IN88US=> NFHJcpRUSU6JRWK=
UACC-62 NYGzTo1LT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDwTWM2OD12Mj61OVA2KM7:TR?= NFn1bodUSU6JRWK=
C-33-A MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjMdYxKSzVyPUS1MlMzPTZizszN NVfrc4ZwW0GQR1XS
HCC1937 NW\0U|NbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3:2RWlEPTB;NEeuNVk2QCEQvF2= NYjQbGtGW0GQR1XS
HDLM-2 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTR5LkKyPFch|ryP MnGzV2FPT0WU
NCI-H727 Mle2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnS2TWM2OD12Nz6yOFUyKM7:TR?= M121ZnNCVkeHUh?=
NY NYTmN5JVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWjvWJllUUN3ME20PU4{QTB6IN88US=> NXjPTGNYW0GQR1XS
NCI-H2291 NUnsbmpKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7K[HdKSzVyPUS5Mlg{ODFizszN M4TVenNCVkeHUh?=

... Click to View More Cell Line Experimental Data

体内研究 Decitabine和Vorinostat都具有使肿瘤衰退的功能。然而, Decitabine 和 Vorinostat 联用使肿瘤衰退的效果更强。没有任何处理时,在野生型和Fasgld 小鼠中在肺肿瘤负担方面观察不到明显的区别。然而, Decitabine 和Vorinostat联用处理野生型小鼠,使肿瘤衰退比处理 Fasgld小鼠效果高很多。 Decitabine和Vorinostat使结肠癌细胞对FasL调节的肿瘤衰退敏感。低剂量Decitabine和Vorinostat处理,没有明显的肝脏毒性。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[4]
+ 展开

组蛋白去乙酰化酶活性测定:

为了进行 HDAC 实验24 × 104 dpm 乙醇溶液(1 mCi/ml)中提供的sup>3H-醋酸钠盐标记的组蛋白H4等份样, 加到每个试管中,试管中含悬浮在25 µL实验buffer(100 mM Tris-HCl pH 8, 2mM EDTA)中的200 µg全部HeLa细胞裂解液进行免疫沉淀获得的HDAC。加入不同浓度Vorinostat,在30oC下温育90分钟。加入20 µL 终止缓冲液(0.5 N HCl, 0.08 M AcOH)终止反应。加入0.8 mL TBME提取释放放入氚醋酸盐。在8,000 × g 转速下离心5分钟,600 µL 有机相(上相) 转移到包含3 mL闪烁液的闪烁瓶中。
细胞实验:[4]
+ 展开
  • Cell lines: NB4, NCI-H460和HCT-116肿瘤细胞系
  • Concentrations: 0 μM - 0.1 μM
  • Incubation Time: 48小时
  • Method: NB4, NCI-H460和HCT-116肿瘤细胞系生长在200 µL 96孔板中,约10%汇合,生长24小时后,覆盖孔中。使用多种浓度 Vorinostat或溶剂处理肿瘤细胞24小时。处理后,冲洗实验板,移除Vorinostat,温育48小时。使用sulphorodamine B 实验测定Vorinostat处理后存活细胞的分数。
    (Only for Reference)
动物实验:[3]
+ 展开
  • Animal Models: 携带CT26 细胞的Fasgld BALB/cByJ小鼠
  • Formulation: Dissolved in DMSO
  • Dosages: 25 mg/kg
  • Administration: 静脉注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 52 mg/mL (196.74 mM)
Ethanol 3 mg/mL (11.35 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 264.3
化学式

C14H20N2O3

CAS号 149647-78-9
稳定性 powder
in solvent
别名 SAHA, MK0683, suberoylanilide hydroxamic acid

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01281176 Active not recruiting Adult Solid Neoplasm National Cancer Institute (NCI) February 9 2011 Phase 1
NCT01189266 Active not recruiting Anaplastic Astrocytoma|Anaplastic Oligoastrocytoma|Brain Stem Glioma|Childhood Glioblastoma|Giant Cell Glioblastoma|Gliosarcoma|Untreated Childhood Anaplastic Astrocytoma|Untreated Childhood Anaplastic Oligoastrocytoma|Untreated Childhood Brain Stem Glioma|Untreated Childhood Giant Cell Glioblastoma|Untreated Childhood Gliosarcoma National Cancer Institute (NCI) August 9 2010 Phase 1|Phase 2
NCT00703664 Completed Recurrent Mantle Cell Lymphoma|Recurrent Non-Hodgkin Lymphoma National Cancer Institute (NCI) July 9 2008 Phase 2
NCT03198559 Suspended HIV Infections The Peter Doherty Institute for Infection and Immunity|Merck Sharp & Dohme Corp.|The Alfred|University of Melbourne August 8 2017 Phase 1|Phase 2
NCT02538510 Active not recruiting Head and Neck Squamous Cell Carcinoma|Recurrent Nasal Cavity and Paranasal Sinus Squamous Cell Carcinoma|Recurrent Nasopharynx Carcinoma|Recurrent Salivary Gland Carcinoma|Squamous Cell Carcinoma Metastatic in the Neck With Occult Primary|Stage III Major Salivary Gland Carcinoma|Stage III Nasal Cavity and Paranasal Sinus Squamous Cell Carcinoma|Stage III Nasopharyngeal Carcinoma|Stage IV Nasopharyngeal Carcinoma|Stage IVA Major Salivary Gland Carcinoma|Stage IVA Nasal Cavity and Paranasal Sinus Squamous Cell Carcinoma|Stage IVB Major Salivary Gland Carcinoma|Stage IVB Nasal Cavity and Paranasal Sinus Squamous Cell Carcinoma|Stage IVC Major Salivary Gland Carcinoma|Stage IVC Nasal Cavity and Paranasal Sinus Squamous Cell Carcinoma University of Washington|National Cancer Institute (NCI) October 8 2015 Phase 1|Phase 2
NCT01983969 Completed Advanced Cancers|Lymphoma M.D. Anderson Cancer Center November 7 2013 Phase 1|Phase 2

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常见问题及建议解决方法

  • 问题 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • 回答:

    We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

HDAC Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID