Vorinostat (SAHA)

目录号:S1047 别名: MK0683, Suberoylanilide hydroxamic acid, Zolinza 中文名称:伏立诺他

仅限科研使用

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683, Zolinza)是一种HDAC抑制剂,无细胞试验中IC50为~10 nM。Vorinostat 会抑制高效的HPV-18 DNA扩增。

Vorinostat (SAHA) Chemical Structure

CAS: 149647-78-9

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1558.77 现货
RMB 1198.15 现货
RMB 1794.86 现货
大包装 有超大折扣
大剂量询单有大折扣!

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全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Vorinostat (SAHA)发表文献480篇:

产品安全说明书

HDAC抑制剂选择性比较

相关HDAC产品

生物活性

产品描述 Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683, Zolinza)是一种HDAC抑制剂,无细胞试验中IC50为~10 nM。Vorinostat 会抑制高效的HPV-18 DNA扩增。
特性 Vorinostat是广谱HDAC活性抑制剂,抑制I和II类酶 。
靶点
HDAC [1]
(Cell-free assay)
~10 nM
体外研究

Vorinostat是小分子量(<300)线性肟酸,抑制HDAC活性,包括乙酰化组蛋白和非组蛋白的积累,抑制培养细胞的增殖,且抑制肿瘤生长。Vorinostat通过结合到酶的活性位点而抑制 HDAC活性。Vorinostat 抑制多种转化细胞增殖(包括 淋巴瘤,多发性骨髓瘤,白血病,和非小细胞肺癌),与对照组相比抑制生长达50% 时的浓度为0.5 到10μM。Vorinostat作用于淋巴瘤和白血病细胞,包括 Burkitt, B-细胞急性淋巴细胞白血病 (B-ALL), MCL, DLBCL, ATL和T-细胞,不同程度抑制细胞增殖。除了抑制转化细胞增殖, Vorinostat 也抑制正常细胞增殖,通过比较Vorinostat 作用于一组细胞系–正常人非纤维细胞(WI-38)和 SV40巨大T抗原转化WI-38(VA-13细胞)而得到证明。Vorinostat 抑制两种类型细胞增殖,这种作用存在剂量依赖性。但发现Vorinostat 对转化细胞具有选择毒性,包括使肿瘤细胞死亡,而对正常细胞只起抑制作用,不会使其死亡。[1] Genistein 和Vorinostat 联用效果比5-aza和Vorinostat 联用作用于诱导细胞死亡有效很多。Genistein和Vorinostat联用作用于ARCaP-E使增殖降低 80 %,作用于ARCaP-M细胞,增殖降低60 %以上。Genistein和Vorinostat 联用抑制细胞增殖具有协同效应。作用于ARCaP-E 细胞(820个基因 诱导和1046个基因抑制) ARCaP-M 细胞,Vorinostat比Genistein 影响更多基因。[2] Vorinostat作用于肿瘤细胞表面,提高Fas蛋白水平,这种作用存在剂量依赖性,且按0.75 μM剂量处理,提高达到一个稳定水平。[3] Vorinostat 抑制肿瘤细胞生长,包括NB4, H460 和HCT-116,IC50分别为0.7μM, 3.4μM和1.2 μM。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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Friend leukemic cell NGTZN3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{ntR|UxKM7:TR?= NV;ZUpBHPzJiaB?= NUjlOYkzTE2VTx?= MXzJR|UxRTBwOUmg{txO MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjFyOUmxN{c,OTJzMEm5NVM9N2F-
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NCI-H226 MoPaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfHc3o1QCCq NX;NTW9xUUN3ME2yMlYh|ryP NGTTc4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexOVQ4OCd-MUW3NVU1PzB:L3G+
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CEM NXLsdpFEU2mwYYPlJGF{e2G7 NG\OOJgyKGh? MUPEUXNQ MWDJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwM{Og{txO MnfaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ2MkCwOlQoRjF4NEKwNFY1RC:jPh?=
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GM15850 M3PNW2tqdmG|ZTDBd5NigQ>? NWP4emZzOi53IN88US=> NIT4cZg6PiCq MXnEUXNQ M{DvVWlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFizJIx6e2mwZTCxOEBi[2W2eXzheIlwdiCxZjDGXG4h\2WwZR?= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
GM15850 M1v3PGtqdmG|ZTDBd5NigQ>? MUCyMlUh|ryP M1m0dlk3KGh? M{P6SWROW09? NVHmfY1IUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFUh[WOndInsZZRqd25ib3[gSnhPKGenbnW= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
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GM15850 NIHnZlhMcW6jc3WgRZN{[Xl? MYCyMlUh|ryP Ml[yPVYhcA>? MYPEUXNQ MYHJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKNDDsfZNqdmViODDhZ4V1gWyjdHnvckBw\iCIWF6g[4Vv\Q>? M37SPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN|Y4Lz5zNkmyNVM3PzxxYU6=
GM15850 NU[0dFVNU2mwYYPlJGF{e2G7 MUmyMlUh|ryP Mk\QPVYhcA>? M{fzbmROW09? MlrJTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSESgcJl{cW6nIEG2JIFk\XS7bHH0bY9vKG:oIF\YUkBo\W6n NE\YfWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
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U937 MnG1SpVv[3Srb36gRZN{[Xl? MU[xJO69VQ>? NFHlRZYzPCCq MlzISG1UVw>? Ml7ySYZn\WO2IH;uJIFteGijIIT1ZpVtcW5iYXPleJlt[XSrb36= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR2N{e1NEc,OTd2NEe3OVA9N2F-
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RAW264.7 NY\5[lJJTnWwY4Tpc44hSXO|YYm= M1XlflExKM7:TR?= M2XwOVI1cA>? MnXoe4F1\XJ? MkjvTY5pcWKrdHnvckBw\iCOUGOtd5RqdXWuYYTl[EBPVyCycn;keYN1cW:wIIfpeIghUUN3MDDv[kAyNjh6IN88US=> NIT2RmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S3O|UyQCd-MUe0O|c2OTh:L3G+
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ST14A MXPGeY5kfGmxbjDBd5NigQ>? MYi1JI5O NH[wNJU4OiCq NF7LWXdKdmO{ZXHz[UBqdiCHMl[xJI1TVkFiZYjwdoV{e2mxbh?= NX;uOJlGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1OlU6QTNpPkG3OVY2QTl|PD;hQi=>
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PT45 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFXtbVhKSzVyPUSg{txO NXS4dWNFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFg4PDRpPkG3OVg5PzR2PD;hQi=>
ACHN NUfwW5V6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLtc2ozOSCq NX:4Zpl[T0l3ME2wMlY2KM7:TR?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ4Mk[wOkc,OTd4NkK2NFY9N2F-
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Caco-2 NWfW[JVDU2mwYYPlJGF{e2G7 NFPGOpA3NjN{IH7NJJRwKDZ|MjFOwG0> M4\iXVQhcA>? M3HvTmROW09? MW\FfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzdid3n0bEBKSzVyIH;mJFQyKM7:TR?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2 M4raUGtqdmG|ZTDBd5NigQ>? M4HvO|AvOzF4IH7NJJRwKDNzLk[g{txO NGjZR2w1KGh? Mm[0SG1UVw>? MUTFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzZ? MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2 MYfLbY5ie2ViQYPzZZk> NHTaU|g2KM7:TR?= MorNOog> NX3UOFU6TE2VTx?= NFK1Rm9KdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBpcXO2b37lJIFk\XS7bHH0bY9v NFzke2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|g1OCd-MkCxOFM5PDB:L3G+
HepG2 NHjrW2lMcW6jc3WgRZN{[Xl? NF7XVpA2KM7:TR?= NWfoN4M2Pmh? M4r1WWROW09? M4i1PWlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgbJlx\XKjY3X0fYxifGmxbh?= M2XMV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N|g1ODxxYU6=
HepG2 NIrqU2dMcW6jc3WgRZN{[Xl? MXy1JO69VQ>? NWrqUXBqPmh? MkLFSG1UVw>? NF;xNGpKdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBidHCqYT30eYJ2dGmwIHHj[ZR6dGG2aX;u NXy4R5BvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
HepG2 MmW4T4lv[XOnIFHzd4F6 NILwNmQ2KM7:TR?= MnzJOog> NF7FToVFVVOR Mk\mTY5lfWO2aX;uJI9nKEiGQVOtcYVlcWG2ZXSgTFMwUDRiaHnzeI9v\SCjY3X0fYxifGmxbh?= NGXPXm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|g1OCd-MkCxOFM5PDB:L3G+
COLO205 MkDGT4lv[XOnIFHzd4F6 MWKxNEDPxE1? MUKyOEBp NF\JSoxFVVOR NHq2[JZKdmirYnn0bY9vKG:oIFjERWMyKGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBpcXO2b37lJGg{KGGlZYT5cIF1cW:wIIfpeIghUUN3MDDv[kAzNjJizszN MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR3MUO3PEc,OjB2NUGzO|g9N2F-
SCC4 NYPZXVJiU2mwYYPlJGF{e2G7 MkmwNUDPxE1? NFXxVmIzPGh? MmWwTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIIT1ZpVtcW5iYXPleJlt[XSrb36= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR3MkKyOUc,OjB2NUKyNlU9N2F-
SCC4 NELTNHNMcW6jc3WgRZN{[Xl? NYK1VWh5OSEQvF2= NXLGeHU3OjSq NHX2XG5KdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDhZ4V1gWyjdHnvci=> MmL1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUKyNlUoRjJyNEWyNlI2RC:jPh?=
SCC4 M{W2XmZ2dmO2aX;uJGF{e2G7 M2Du[|Eh|ryP NHT1fYQ2KG2rbh?= MX3B[49vcXO2IHHjeIl3cXS7IHH0JHZFWiCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhS1mSMkSg[4Vv\SCneIDy[ZN{cW:w Mn\kQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUKyNlUoRjJyNEWyNlI2RC:jPh?=
NB4 MmfUSpVv[3Srb36gRZN{[Xl? NF3qT2M2KM7:TR?= NIHIV2MzPCCq NVjTbJpDTE2VTx?= MVLD[YxtKGO7Y3zlJIFzemW|dDDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCjY3P1cZVt[XSrb36gZZQheHKnLVexJJBp[XOn NVqzPJZ3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0PVE1PDBpPkKwOFkyPDRyPD;hQi=>
NB4 M{PCTGtqdmG|ZTDBd5NigQ>? NGDoToM2KM7:TR?= MmTxNlQhcA>? NWPTN3VPTE2VTx?= MVzJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iYXPleJlt[XSnIIT1ZpVtcW5ibHX2[Yw> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR7MUS0NEc,OjB2OUG0OFA9N2F-
NB4 M3H4U2Z2dmO2aX;uJGF{e2G7 NFKxe5E2KM7:TR?= Mm\LNlQhcA>? MVjEUXNQ MXrJcoR2[3Srb36gc4YheDJzV1HGNU9EUVBzIHX4dJJme3Orb36= NYLCVm1URGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0PVE1PDBpPkKwOFkyPDRyPD;hQi=>
NB4 M{LaRWtqdmG|ZTDBd5NigQ>? NYT0fmwzPSEQvF2= NETrc5UzPCCq M1fFcmROW09? NFr5VmlKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIN{Bi[2W2eXzheIlwdg>? M1\tdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEmxOFQxLz5{MES5NVQ1ODxxYU6=
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A549 NV7M[5Z{TnWwY4Tpc44hSXO|YYm= M2XWc|Uh|ryP NV\DO4MyOyCq MWXEUXNQ MkLlTY5lfWO2aX;uJI9nKGG3dH;wbIFogSCrbjDoeY1idiCDNUS5JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEyFM1nJ MnnvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6NUWyNFgoRjJyOEW1NlA5RC:jPh?=
HUT78 NYnMWFlDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jhdlczKGh? NXXC[XZzTE2VTx?= MWfHTVUxRTBwMzFOwG0> Mo\rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6OESyNFgoRjJyOEi0NlA5RC:jPh?=
U937 MoHTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4CxdFczKGh? M2H5OGROW09? Ml\RS2k2OD1yLkO5JO69VQ>? NHXUPVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi4OFIxQCd-MkC4PFQzODh:L3G+
A431 MmOxR5l1d3SxeHnjJGF{e2G7 M1;nOFczKGh? MmDkR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVQ{OSClZXzsd{BwfmW{ZYjwdoV{e2mwZzDFS2ZTKHerdHigTWM2OCCxZjC0MlIh|ryP M1nVTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlI6Lz5{MUC4NFYzQTxxYU6=
A549 M{jXVGN6fG:2b4jpZ{BCe3OjeR?= NFLiNFI4OiCq NULZXHVpS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCxdnXy[ZhxemW|c3nu[{BJTEGFIIfpeIghUUN3MDDv[kAyNjhizszN NFHz[ng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
CAL27 Ml31R5l1d3SxeHnjJGF{e2G7 NFHycWQ4OiCq MVTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDRWwzPyClZXzsd{BwfmW{ZYjwdoV{e2mwZzDFS2ZTKHerdHigTWM2OCCxZjCzMlIh|ryP NIrDPFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
HeLa MkLLR5l1d3SxeHnjJGF{e2G7 M3yzZlczKGh? M1TySWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMhd3[ncnX4dJJme3OrbnegTGRCSyC5aYToJGlEPTBib3[gNU42KM7:TR?= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
Sf21 NYfpXplkU2mwYYPlJGF{e2G7 M4K2OVMhcA>? MojiTY5pcWKrdHnvckBw\iCIbHHnJJRi\2enZDDoeY1idiC{ZXPvcYJqdmGwdDDISGFEQCCneIDy[ZN{\WRid3n0bEBKSzVyIH;mJFEvOiEQvF2= NXjJdWpJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3OjlpPkKxNFgxPjJ7PD;hQi=>
SKBR3 MUXDfZRwfG:6aXOgRZN{[Xl? NGXoUno4OiCq MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT2JTOyClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISXIzKHerdHigTWM2OCCxZjCyMlYh|ryP MoX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2NlkoRjJzMEiwOlI6RC:jPh?=
SKOV3 NUWydIl{S3m2b4TvfIlkKEG|c3H5 M2q1S|czKGh? MVLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISXIzKHerdHigTWM2OCCxZjCyMlIh|ryP MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
COR-L23 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX6xNEDPxE1? M{HLUlczKGh? NHLYSZJFVVOR MVHHTVUxRTFwMTFOwG0> NHX4e4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
DU145 M2KzSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUmwfI1SOTBizszN M2rBPFczKGh? M1u2XmROW09? NHvHWWZIUTVyPUGuNFUh|ryP Mn3wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
LoVo MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnG1NVAh|ryP M3PGU|czKGh? MnnFSG1UVw>? MnvkS2k2OD1zLkeg{txO MoXlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
MCR5-SV2 NH\UUGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHldVQyOCEQvF2= MnTrO|IhcA>? NEXkZnVFVVOR MX\HTVUxRTJwMTFOwG0> NHj2Rpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
HCT116 M1T3N2tqdmG|ZTDBd5NigQ>? NUXKXJhzOzBizszN MnnsN|AhcA>? MnOwSG1UVw>? NVLKbG84UW6qaXLpeIlwdiCxZjDISGFEPiCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[WyyaHGteJVjfWyrbjDhZ4V1gWyjdHnvci=> NITU[|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
HCT116 NHfyOFBMcW6jc3WgRZN{[Xl? MWmzNEDPxE1? MoL6N|AhcA>? NF;3NJlFVVOR MXTJcohq[mm2aX;uJI9nKEiGQVOxJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDobZN1d26nIFizT|kh[WOndInsZZRqd25? MlX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
U937 NEHJNlNMcW6jc3WgRZN{[Xl? MYK1JO69VQ>? MlXQNlQhcA>? MWPEUXNQ MonLTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6|ZnXyZZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJIFk\XS7bHH0bY9vKG:oIHjpd5RwdmViSEOgZZQhUzl? NX7WdnZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVI1QTJpPkKxNlkzPDl{PD;hQi=>
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U937 MWDLbY5ie2ViQYPzZZk> NUXGZXdQPSEQvF2= NVHUT5RYOjRiaB?= MUXEUXNQ NVHXWGx2UW6qaXLpeIlwdiCxZjDobZN1d26nIHHj[ZR6dHS{YX7z[oVz[XOnIHHzd4V{e2WmIHHzJIlv[3KnYYPlJI9nKHCjbj3hZ4V1gWyjdHXkJIhqe3SxbnWgTFQhdGW4ZXy= NWm1fo5tRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVI1QTJpPkKxNlkzPDl{PD;hQi=>
U937 NGrZcZBMcW6jc3WgRZN{[Xl? M4HCdlUh|ryP NHjMXI4yPSCvaX6= NIjLWHFFVVOR MnvtTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6|ZnXyZZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJIhqe3SxbnWgTFMh[WOndInsZZRqd25ibHX2[Yw> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MkS5Nkc,OjF{OUK0PVI9N2F-
A2780 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LKZ|k3KGh? M4rIOGROW09? NH7uTVhKSzVyPUGuOlIh|ryP NV3Wbo4yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2N|Q1OzBpPkKxOlM1PDNyPD;hQi=>
COLO205 NG\HfFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHRPZhSQTZiaB?= MlTmSG1UVw>? M3r3NGlEPTB;Mj6xNkDPxE1? NGnjZWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[zOFQ{OCd-MkG2N|Q1OzB:L3G+
Hep3B NVHMVVdIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX23VpB[PDhiaB?= MljRSG1UVw>? NE\OVIhIUTVyPUGuNlEh|ryP M1\MdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GyNVQ3Lz5{MUexNlE1PjxxYU6=
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UO31 M4\yVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LQfVYvOiEQvF2= MXW3NkBp MoX3SG1UVw>? M4\icGdKPTB;MD61JO69VQ>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
Hep3B NWWwVoM1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGf4eVY1QCCq NUfqZVE3TE2VTx?= NVzHeZNwT0l3ME2wMlY6KM7:TR?= M2PUdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO5PFY{Lz5{MkSzPVg3OzxxYU6=
BE(2)-C NXnVeZJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2O4e|czKGh? NXu3VIpQTE2VTx?= M{XvcmlEPzB;MjFOwG0> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl|MkOxOkc,OjJ7M{KzNVY9N2F-
Bel7402 NHvoUZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzSbG9QPzJiaB?= MYHEUXNQ MVTHTVkxRTF5IN88US=> M4jTeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
Bel7404 NGXscmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPKO|IhcA>? NF\XW4FFVVOR MUfHTVkxRTF6IN88US=> M4mzbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
BGC823 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXu3NkBp MlWySG1UVw>? MkPBS2k6OD15LkWg{txO MlLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkGzO|YoRjJ|ME[xN|c3RC:jPh?=
MGC803 NV;hfG51T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXfRdIRWPzJiaB?= NGDFbmRFVVOR MVTHTVkxRTFyLkig{txO NXjPTo16RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlE{PzZpPkKzNFYyOzd4PD;hQi=>
SGC7901 MkLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLOO|IhcA>? NWG4RXI3TE2VTx?= NWf5do9DT0l7ME25MlQh|ryP M{PKVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
SKHEP1 NIruUpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorpO|IhcA>? MWPEUXNQ NVXMbVFET0l7ME2xO{DPxE1? NELGOmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SMMC7721 M1HkNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3m0cVczKGh? NVrvU483TE2VTx?= NXzOeIRYT0l7ME2yNEDPxE1? NYHhe3RJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlE{PzZpPkKzNFYyOzd4PD;hQi=>
NUGC3 NFfkN2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4LUTVExKM7:TR?= MmDJS2k:Oi57NDFOwG0> MorqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNkOzN|IoRjJ|MU[zN|MzRC:jPh?=
A2780 CisR NXfvW2NOU2mwYYPlJGF{e2G7 M{jFNVE5KGh? M2\P[2lvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD60OEDPxE1? NUT6WGNpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
CAL27 sens M3TWZ2tqdmG|ZTDBd5NigQ>? NIO4XXEyQCCq MXvJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwM{[g{txO NHrJcoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
CAL27 CisR Ml\yT4lv[XOnIFHzd4F6 NXjveGJtOThiaB?= NHjTZ4NKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOlEh|ryP MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
KYSE-510 CisR NYfjW3ZxU2mwYYPlJGF{e2G7 MlTTNVghcA>? NVHjdnhzUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlU6KM7:TR?= NX2w[IFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
KYSE-510 sens M{n2XWtqdmG|ZTDBd5NigQ>? NIntTlAyQCCq NXvaNlNYUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlch|ryP MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
MDA-MB-231 sens Mm\ZT4lv[XOnIFHzd4F6 MniyNVghcA>? M3\NTmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD62NUDPxE1? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
MDA-MB-231 CisR NVL5OWZMU2mwYYPlJGF{e2G7 M4nSXlE5KGh? MV3Jcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNkGg{txO M1vNSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
ES2 M3jpd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnBVItiPDhiaB?= NFHjXY1FVVOR NVXRfJdCUUN3ME2xNk44KM7:TR?= NWniWGJoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
MDA-MB-231 NYr1VYJuU2mwYYPlJGF{e2G7 M{TySlExKM7:TR?= NIXRb5E{OCCvaX6= M3LqVWROW09? M3rFXmlvcGmkaYTpc44hd2ZiSFTBR|Ji NVP4do9vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
MDA-MB-231 MnflT4lv[XOnIFHzd4F6 M13TNFEh|ryP NYDQ[lI{OjRiaB?= MXTEUXNQ NELpRmpKdmirYnn0bY9vKG:oIFjERWM3KGG|c3Xzd4VlKGG|IHHj[ZR6dGG2aX;uJIxmfmWuczDv[kB1fWK3bHnu MnjyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUO0OFkoRjJ|NEmzOFQ6RC:jPh?=
HS68 NH:4PYVEgXSxdH;4bYMhSXO|YYm= MXSxNEDPxE1? NGT1Wpk4OiCq Mmn4SG1UVw>? NFLUXGlKSzVyPUSuOkDPxE1? NWq5T|c2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|A2PDJpPkKzOVcxPTR{PD;hQi=>
MEF MYfDfZRwfG:6aXOgRZN{[Xl? MlLlNVAh|ryP MoLrO|IhcA>? MUfEUXNQ NFzjepJKSzVyPUSuOEDPxE1? NGHqco09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W3NFU1Oid-MkO1O|A2PDJ:L3G+
BGC823 NUPofI5yT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEP2bFdFVVOR MWTHTVUxRTNwMEmg{txO NWq1W41wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NFE4ODZpPkKzOlAyPzB4PD;hQi=>
High5 MnOzT4lv[XOnIFHzd4F6 M1HtTFI1KGh? NFzjTpFKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWM3KHerdHigTWM2OCCxZjCwMlA6KM7:TR?= MlzpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MEK1NlMoRjJ|NkCyOVI{RC:jPh?=
High5 MVHLbY5ie2ViQYPzZZk> MVKyOEBp NVvMXoNLUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOSC5aYToJGlEPTBib3[gNE4yOiEQvF2= NE\TelA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[wNlUzOyd-MkO2NFI2OjN:L3G+
High5 M1nnVWtqdmG|ZTDBd5NigQ>? MnW3NlQhcA>? Mo\ZTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDN{B4cXSqIFnDOVAhd2ZiMD6xO{DPxE1? NWHM[VNzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NFI2OjNpPkKzOlAzPTJ|PD;hQi=>
A549 MknuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\WUlRFVVOR NF6xZY5KSzVyPUGuNFc6KM7:TR?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ2NEKxNEc,OjN4NESyNVA9N2F-
HCT116 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPMVVVsTE2VTx?= NVrxSZhMUUN3ME2wMlc2PyEQvF2= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ2NEKxNEc,OjN4NESyNVA9N2F-
K562 M3\keGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWXKcVk{TE2VTx?= M1PPbGlEPTB;MD62OFUh|ryP NITEPYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyOCd-MkO2OFQzOTB:L3G+
GBC-SD NVjGSJZ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGDrSZU2KM7:TR?= NXnCR|RvPzJiaB?= NEjEXYJFVVOR NUHESpBmUUN3ME2xOE45KM7:TR?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{K2NEc,OjN5MEeyOlA9N2F-
HGC27 NUPadGRKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fZW|Uh|ryP NETsV5Y4OiCq MkTVSG1UVw>? NUnOcYR4UUN3ME21MlQh|ryP M3rCUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C3NlYxLz5{M{ewO|I3ODxxYU6=
HL60 M4\sV2tqdmG|ZTDBd5NigQ>? M3XxT|Uh|ryP M3fRb|I1KGh? Mn7CSG1UVw>? MUDJcohq[mm2aX;uJI9nKEiGQVO= NWnmOG1VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFczPjBpPkKzO|A4OjZyPD;hQi=>
Vero NGXKTXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jqSFczKGh? NUCxOoR2TE2VTx?= NHzuW2VKSzVyPUCuPUDPxE1? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6NkS1Nkc,OjN5OE[0OVI9N2F-
NCI60 NWX5[GhLS3m2b4TvfIlkKEG|c3H5 MnXwOFghcA>? M{WwXWROW09? M3fRZ2dKRTBwNUOg{txO M3nKVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N|I4Lz5{NECxOVMzPzxxYU6=
SW620 M2PYdWN6fG:2b4jpZ{BCe3OjeR?= NF7GNoQ1QCCq NFXyO4lFVVOR MkjkS2k:OC53NDFOwG0> NEP1dYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxOVMzPyd-MkSwNVU{Ojd:L3G+
U251 MnzCR5l1d3SxeHnjJGF{e2G7 NF7NNpg1QCCq MWLEUXNQ NVfS[lVMT0l;MT61N{DPxE1? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
KM12 MoHLR5l1d3SxeHnjJGF{e2G7 NF7DUGM1QCCq MVvEUXNQ NH7t[pNIUT1zLki4JO69VQ>? M2ruOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N|I4Lz5{NECxOVMzPzxxYU6=
Hs578T M3v3Z2N6fG:2b4jpZ{BCe3OjeR?= NXvpZ|kyPDhiaB?= MV3EUXNQ NYXqcIdWT0l;ND64N{DPxE1? NVvUW5MxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVU{OjdpPkK0NFE2OzJ5PD;hQi=>
MDA-MB-231 MkTRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjTdnA1QCCq Mm\TTWM2OD1yLkG2JO69VQ>? NHjYNlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5OVAyPid-MkSwPVUxOTZ:L3G+
MCF7 NHHnco1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWG0PEBp MX7HTV0xNjR3IN88US=> MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFzOUW1OUc,OjRzMUm1OVU9N2F-
MDA-MB-435 NFLHT2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnZU5dSPDhiaB?= MojCS2k:OC56ODFOwG0> NXjycXpwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
AsPC1 NH7NfWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrYRoc{OCEQvF2= M3\kVlQ5KGh? MkLXSG1UVw>? M3G5SGlEPTB;Mz62OkDPxE1? MkHRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOEWzO|goRjJ2MUi1N|c5RC:jPh?=
Jurkat E6.1 M3[3UmtqdmG|ZTDBd5NigQ>? MVKyJIg> NWP2eGRoTE2VTx?= M1H4bWlvcGmkaYTpc44hd2ZiSFTBR|Qhf2m2aDDJR|UxKG:oIECuNFQh|ryP NGDseWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2NVg3Oid-MkSyOlE5PjJ:L3G+
KG1 NFv3TFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGqwZXM1QCCq NYrHT49lUUN3ME2xMlE2KM7:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{NUCwN{c,OjR3MkWwNFM9N2F-
HEL MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MorvOFghcA>? MVjEUXNQ NFLTe5NKSzVyPUCuOFkh|ryP MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7NEC1OUc,OjR4OUSwOVU9N2F-
HSC3 NEW3c3JEgXSxdH;4bYMhSXO|YYm= NHjh[3gyOiCq M1[5UmROW09? MVvJR|UxRTBwN{Gg{txO NHPUVno9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
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NCI-H2347 NIC3TFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\YT|lKSzVyPUKzMlI3PzRizszN NFu5O5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
U-118-MG NXu0SnJsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HSW2lEPTB;MkOuO|IyPyEQvF2= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NCI-H596 NITwSZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFq2WZhKSzVyPUKzMlkxPTVizszN NILMVY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
MZ1-PC MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfoTY1pUUN3ME2yOE43PzV7IN88US=> M{W2WVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
SW1417 Mnf0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXHTRZg6UUN3ME2yOU4zPDl6IN88US=> NEL1UnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H82 MnS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3e2WGlEPTB;Mk[uOFI5QCEQvF2= NXvlRYNqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
COLO-678 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFPXVVJKSzVyPUK3MlQ6OTlizszN M{jVU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
KURAMOCHI MojhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVzJR|UxRTJ6LkGyOkDPxE1? NV3x[5FZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H1650 NHvUU25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVn1c2ZqUUN3ME2zNE4yQTJ2IN88US=> M4DmXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
HCC1954 NX36TlI3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTNzLkO2OlYh|ryP NIjaXHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
GAK Mkn1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGrifWZKSzVyPUOyMlI3OTZizszN M3jReFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
YAPC NW\EXYlJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLh[XEyUUN3ME2zN{44PjR6IN88US=> NHjiUnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H2228 NXfXSGhTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTN2LkO2NFUh|ryP MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
VMRC-RCZ NXz1TnhbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHH[|hlUUN3ME2zOk4{ODJ{IN88US=> MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NMC-G1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTN5LkC1Nlkh|ryP M3vWS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
D-336MG NYrkcGE3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPlcI1sUUN3ME20NU45PDR{IN88US=> NHXmfFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
UACC-62 NWfHXVhzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HMbWlEPTB;NEKuOVUxPSEQvF2= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
C-33-A MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTR3LkOyOVYh|ryP MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
HCC1937 NGS3RphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn;1TWM2OD12Nz6xPVU5KM7:TR?= NEXpU4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HDLM-2 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjuc4FKSzVyPUS3MlIzQDdizszN NWHuTox4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H727 NHK3TpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTR5LkK0OVEh|ryP MonEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NY NUTyPGZbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoXUTWM2OD12OT6zPVA5KM7:TR?= NXe4bpdLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H2291 NH\ENGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rIUWlEPTB;NEmuPFMxOSEQvF2= NVLTSVBtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
Assay
Methods Test Index PMID
Western blot Ac-Histone H4 / Ac-Histone H3 phospho-CDK1 phospho-Cdc25c / Cdc25c Cyclin B1 c-Myc / c-Raf / Akt / Cyclin D1 / CDK4 p-eIF2α / ATF4 / CHOP p21 21598070 19440035 20072656
Immunofluorescence HDAC1 / CK19 Nrf2 α-SMA Ac-STAT3 / IGF2 29917299 20566897 26330748 27086926
Growth inhibition assay Cell apoptosis Cell viability 19440035 27086926
ELISA IGF2 IL-13 / IL-10 / IL-5 M-CSF MMP-9 27086926 18541724 23042302
体内研究 Decitabine和Vorinostat都具有使肿瘤衰退的功能。然而, Decitabine 和 Vorinostat 联用使肿瘤衰退的效果更强。没有任何处理时,在野生型和Fasgld 小鼠中在肺肿瘤负担方面观察不到明显的区别。然而, Decitabine 和Vorinostat联用处理野生型小鼠,使肿瘤衰退比处理 Fasgld小鼠效果高很多。 Decitabine和Vorinostat使结肠癌细胞对FasL调节的肿瘤衰退敏感。低剂量Decitabine和Vorinostat处理,没有明显的肝脏毒性。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[4]
  • 组蛋白去乙酰化酶活性测定:

    为了进行 HDAC 实验24 × 104 dpm 乙醇溶液(1 mCi/ml)中提供的sup>3H-醋酸钠盐标记的组蛋白H4等份样, 加到每个试管中,试管中含悬浮在25 µL实验buffer(100 mM Tris-HCl pH 8, 2mM EDTA)中的200 µg全部HeLa细胞裂解液进行免疫沉淀获得的HDAC。加入不同浓度Vorinostat,在30oC下温育90分钟。加入20 µL 终止缓冲液(0.5 N HCl, 0.08 M AcOH)终止反应。加入0.8 mL TBME提取释放放入氚醋酸盐。在8,000 × g 转速下离心5分钟,600 µL 有机相(上相) 转移到包含3 mL闪烁液的闪烁瓶中。

细胞实验:[4]
  • Cell lines: NB4, NCI-H460和HCT-116肿瘤细胞系
  • Concentrations: 0 μM - 0.1 μM
  • Incubation Time: 48小时
  • Method: NB4, NCI-H460和HCT-116肿瘤细胞系生长在200 µL 96孔板中,约10%汇合,生长24小时后,覆盖孔中。使用多种浓度 Vorinostat或溶剂处理肿瘤细胞24小时。处理后,冲洗实验板,移除Vorinostat,温育48小时。使用sulphorodamine B 实验测定Vorinostat处理后存活细胞的分数。
动物实验:[3]
  • Animal Models: 携带CT26 细胞的Fasgld BALB/cByJ小鼠
  • Dosages: 25 mg/kg
  • Administration: 静脉注射

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O

5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 264.3
化学式

C14H20N2O3

CAS号 149647-78-9
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03022565 Withdrawn Drug: Vorinostat Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center January 2020 Early Phase 1
NCT03167437 Recruiting Drug: Vorinostat|Drug: Ustekinumab Crohn''s Disease National Institute of Allergy and Infectious Diseases (NIAID)|National Institutes of Health Clinical Center (CC) October 30 2017 Phase 1|Phase 2
NCT03056495 Recruiting Drug: N-hydroxy-N''-phenyl-octanediamide (Vorinostat) Alzheimer Disease German Center for Neurodegenerative Diseases (DZNE)|University Hospital Bonn|University of Göttingen September 28 2017 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

技术支持

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操作手册

如果有其他问题,请给我们留言。

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常见问题及建议解决方法

问题 1:
What’s the recommendation about reconstitution of the compound for in vivo animal study?

回答:
We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.