Vorinostat (SAHA, MK0683)

目录号：S1047 别名： SAHA, MK0683, suberoylanilide hydroxamic acid

Vorinostat (SAHA, MK0683) Chemical Structure

Molecular Weight(MW): 264.3

Vorinostat (suberoylanilide hydroxamic acid, SAHA)是一种HDAC抑制剂，无细胞试验中IC50为~10 nM。

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客户购买Selleck的此次产品后发表的文献103篇：

客户使用该产品的20个实验数据：

  • Activity of vorinostat in Jurkat and Peer T-ALL cell lines in an MTT cell viability assay. Cells were treated with increasing drug concentrations for 72 h. The data are plotted as the mean % of DMSO-treated control cells against the corresponding drug concentration. The error bars are the standard error. For each drug and cell line, 3-4 independent experiments were performed with 6 replicates at each drug concentration.

    Nature 2011 471, 235-9. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Differential effects of HDAC inhibitors on histone and tubulin acetylation. Immunofluorescence analysis of histone H3 (K9ac/K14ac) and tubulin acetylation in HeLa cells treated for 4 h with vehicle, SAHA (10 μM), tacedinaline (50 μM), PCI-24781 (20 μM. (a) Mapping of histone acetylation in K562 cells treated with HDAC inhibitors by LC-MS/MS. Cells were treated with TSA (10 μM), SAHA (5 μM), PCI-24781 (2 μM), tacedinaline (50 μM) for 6 h. Histones were extracted from cells and acetylated peptides were quantified after isobaric tagging.

    Nat Biotechnol 2011 29, 255-265. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Western blot analysis of histone H3 acetylation in the spleen of untreated and vorinostat-treated hNF-E2 tg mice (n = 4 of each genotype).

    J Exp Med 2012 209, 35-50. Vorinostat (SAHA, MK0683) purchased from Selleck.

    SAHA down-regulates c-FLIP expression and increases the sensitivity of CaP cells to undergo apoptosis in the presence of bicalutamide (A) Bar graph illustrating the sub-G0/G1 cell population in 22Rv1 (left panel) and LNCaP (right panel) cells in the absence and presence of 10μM bicalutamide, administered as a single agent or in ombination with increasing concentrations of the HDAC inhibitor, SAHA. (B) Immunoblots demonstrating the single agent activity of bicalutamide or SAHA, or effect upon their combined administration on the expression of c-FLIP and/or the processing of PARP. Equal protein loading was confirmed by GAPDH. (C) Bar graph illustrating the effect of bicalutamide and SAHA upon the induction of caspase-8 or caspase-3/7 activity in 22Rv1 cells (left panel) and LNCaP cells (right panel). (D) Immunoblots characterizing the impact of SAHA and/or bicalutamide upon the induction of PARP cleavage, in the absence or presence of the pan-caspase inhibitor, ZIETD. ZIETD was incubated with the cells for 12 h prior to the experiment. All data points shown in (A) and (C) represent the mean ± S.E.M. value, calculated from four independent experiments.

    Clin Cancer Res 2012 18, 3822-33. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Analyses of efficacy, potency and IC50 of HDAC inhibitors point toward HDACs 1–3 as relevant candidates for beta cell protection. Ranking and raw data on ITF drugs (Table 3) and commercial HDAC inhibitors (Table 4) underlying the heat maps of Fig. 1 (A and B). The HDAC inhibitor compounds were tested using a HDAC activity kit and recombinant proteins to determine IC50 values on each individual HDAC (right part of the table). Each drug was further tested using Real-Time Cell Analysis (RTCA) to score their maximal effective concentration (ECmax) as well as the corresponding rescue percentage of cytokine treated INS-1 cells. The drugs were ranked according to % rescue. * DMSO alone controls were included in each experiment, but not shown here. The results indicate that the highest concentrations of DMSO used here (1:1,000) were slightly potentiating the proliferation of the cells. The effects observed in this group of compounds were ascribed to the DMSO.?

    Diabetologia 2012 55, 2421-2431. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Suberoylanilide hydroxyamic acid (SAHA) preserves CFP (+) expression in mouse optic nerves (MONs) from older mice. (a) CFP (+) mitochondria were longer and thicker in MONs from 12-month-old Thy-1 CFP mice (inset; scale bar = 2 μm). Oxygen–glucose deprivation (OGD) consistently reduced CFP pixel intensity in 12-month-old MONs despite longer and brighter CFP ( +) mitochondria (yellow arrows). (b) Blockade of AMPA/kain ate receptors with NBQX (30 μM), or pan-Histone deacetylase (HDAC) inhibition with SAHA (5 μM), effectively preserved CFP pixel intensity during OGD. The preservation of CFP (+) mitochondria correlates with protection of the CAP area during OGD and profound recovery.

    J Neurochem 2012 123 Suppl 2, 108-15. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • D and E, Regression of IL-13Ra 2-negative pancreatic tumors treated with SAHA and/or IL-13-PE. Mice were treated daily with i.p. injection of SAHA (25 mg/kg) from day 4 after tumor implantation for two weeks followed by i.t. injection of IL-13-PE (100 μ g/kg) from day 5 for two weeks. F and G, Regression of IL-13Ra 2-posotive pancreatic tumors (HS766T and MIA-PaCa2) treated with SAHA and/or IL-13-PE. The schedule of treatment was similar as in panel D and E. Statistical significances are shown by *: P < 0.05,†: P < 0.001.

    J Transl Med 2011 9, 37. Vorinostat (SAHA, MK0683) purchased from Selleck.

    IL-13Rα2 expression is upregulated in pancreatic tumors but not in organs of mice after treatment with HDAC inhibi tor, SAHA. A, qRT-PCR of human IL-13Rα2 in implanted pancreatic tumors after SAHA and IL-13-PE treatment. Tumors were harvested next day after IL-13-PE treatment and total RNA extracted. Data shown is ratio of human IL-13Rα2/β-actin expression and multiplied by 1000 for convenience. Bars , SD of triplicate determinations. B, qRT-PCR of mouse IL-13Rα2 in mice organs after SAHA and IL-13-PE treatment. Tissues were harvested at the same time point as in panel A and total RNA extracted. Data shown is ratio of mouse IL-13Rα2/β-actin expression and multiplied by 100 for convenience. C, IHC of human IL-13Rα2 in implanted pancreatic tumors after SAHA and IL-13-PE treatment. D, IHC of mouse IL-13Rα2 in mice organs after SAHA and IL-13-PE treatment. Liver, brain, kidney, pancreas, lung and spleen were fixed for immunostaining of mouse IL-13Rα2 as visualized by Alexa555. Nucleus was counterstained by DAPI.

    J Transl Med 2011 9, 37. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Proliferation o f HT29 and HCT116 cells after 48 h of butyrate or SAHA treatment. Proliferation measurements using the xCEL Ligence RTCA DP instrument (Roche) in cells treated with increasing doses of butyrate or SAHA, compared with control medium cells (0) (*P < 0.05). The mean ?SEM of three cell culture replicates is shown.

    Mol Carcinog 2012 52, 459-74. Vorinostat (SAHA, MK0683) purchased from Selleck.

    SAHA induces the down-regulation of cFLIP expression and promotes TRAIL-mediated activation of a mitochondrial-operated apoptotic pathway. a BT-474 cells were treated as indicated in Fig. 1 and the cells were then harvested to analyse caspase-8, Bid, caspase-9, caspase-3 and PARP expression, using GAPDH as the protein loading control. b BT-474 cells were treated with SAHA for 8 h at the doses indicated and subsequently, the cells were harvested and the apoptosis-related proteins were analysed in western blots as described in the "Materials and Methods". The results shown are representative of at least three independent experiments

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Treatment with SAHA sensitizes breast cancer cells to TRAILinduced apoptosis. BT-474 and MDA-MB-231 cells were incubated for 8 h with SAHA at the doses indicated. After this time, TRAIL (500 ng/ml) was added to some of the cultures for an additional 15 h. Apoptosis was measured as the percentage of cells with subG1 DNA content as described in the "Materials and methods". The results show the mean and S.E.M. of three independent experiments

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

    SAHA facilitates the Itch/AIP4-independent proteasomal degradation of cFLIP proteins in breast cancer cells. a BT-474 cells were treated with SAHA (5 μM) for 8 h in the presence or absence of the proteasome inhibitors MG132 or epoxomicin (at the doses indicated), and they were then harvested and the cFLIP content was analysed in western blots. b BT-474 cells were transfected with either the siRNA oligonucleotides targeting Itch/AIP4 or the non-targeting RNA oligonucleotide (scrambled) for 48 h as described in the "Materials and Methods". Subsequently, the cells were treated with SAHA (5 μM) for 8 h and then cFLIP and Itch/AIP4 expression was analysed in immunoblots

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • cFLIP down-regulation following SAHA treatment is responsible for the sensitization of breast cancer cells to TRAIL. a BT-474 cells were transfected with either different siRNAs directed against the cFLIP isoforms or the non-targeting RNA oligonucleotide (scrambled) as described in the "Materials and Methods". After 24 h, the cells were treated with TRAIL (500 ng/ml) for a further 15 h and apoptosis was then measured as the percentage of cells with subG1 DNA content. The results show the mean and S.E.M. of three independent experiments. To verify protein knockdown, cells were harvested 24 h after transfection and the cFLIP levels were assessed in immunoblots. b. BT-474 cells overexpressing Cflipl (pBABE-FLIPL) or control cells (pBABE) were treated with SAHA for 8 h at the doses indicated. After this time, TRAIL (500 ng/ml) was added to some cultures for 15 h. Apoptosis was measured as the percentage of cells with subG1 DNA content and the results show the mean and range of two independent experiments. Western blots show the cFLIP levels after SAHA treatment in pBABE and pBABE-FLIPL cells.

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Vorinostat inhibits the tumor growth. NOD ⁄ SCID-B2m-/-mice were injected s.c. with 1×106 MyLa2059 cells into both flanks. When the mice had developed established palpable tumors treatment was initiated (day 1). The mice received either vehicle (black line) or 60 mg ⁄ kg vorinostat (grey line) i.p. five days a week. In total, there were four mice in the group receiving vehicle and three mice in the group receiving vorinostat. The length and width of the tumors were measured continuously until the experiment was terminated on day 20 post treatment initiation. The tumor volume was calculated using the formula V = (a×b2)/2, where a defines the length (mm) and b the width (mm) of the tumor. The histogram shows the average tumor volume per flank ± SEM. * denotes a significant difference (P < 0.05) in the average tumor volume per flank between mice treated with vehicle and vorinostat at the given time-point both by using a one-tailed two samples test with Welch, scorrection and a Mann-Whitney U test.

     

     

    Exp Dermatol 2010 19, 1096-1102. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • (a) Western blot of whole cell extracts demonstrates SAHA and NaB treatment result in increased levels of acetylated histone H3 compared to vehicle controls. (b) Light micrographs of HDACi/vehicle treated cultures illustrate the paucity of neurospheres in HDACi-treated cultures. (c) Quantitative analysis confirms SAHA and NaB inhibit the formation of neurospheres (diameter ≥ 50 μm) and increase the prevalence of small cell clusters (≥ 4 cell, diameter < 50 μm). (d) Flow cytometry using LIVE/DEAD cell stain confirms SAHA and NaB are minimally toxic compared to vehicle controls (n = 3). Scale bar = 100 μm.

    BMC Neurosci 2011 12, 50. Vorinostat (SAHA, MK0683) purchased from Selleck.

    (a) EdU flow cytometry of live cell-gated adult mouse NSCs demonstrates SAHA (**p < 0.01) and NaB (***p < 0.001) significantly inhibit DNA synthesis. (b) Analysis of DNA content by flow cytometry reveals significant accumulation of adult mouse NSCs in G1phase of the cell cycle following SAHA (*p < 0.05) and NaB (*p < 0.05) treatment. (c) Correspondingly the proportion of cells in G2/M phase is significantly reduced following SAHA (*p < 0.05) and NaB (*p < 0.05) treatment. (d) Fluorescence micrographs of HDACi/vehicle treated adult NSCs pulse-labeled with EdU for flow cytometry. Scale bar = 50 μm. Statistical comparisons performed using one-way ANOVA with post hoc Newman-Keuls multiple comparison tests.

    BMC Neurosci 2011 12, 50. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • (a) Immunofluorescence labeling of NeuN, GFAP and Olig2 cell fate markers reveals reduced expression of GFAP (*p < 0.001) and Olig2 (*p < 0.05) in adult mouse NSCs treated with SAHA for 48 hours under proliferation culture conditions and then induced to differentiate for 14 days in culture (two-way ANOVA with Bonferroni post-tests). (b) Western blot of β-catenin protein in nuclear and cytoplasmic fractions of adult mouse NSCs treated with HDACi/vehicle control under proliferation culture conditions. Gapdh and lamin A/C blots are included as loading controls. (c) SAHA and NaB do not significantly alter nuclear/cytoplasmic levels of β-catenin in adult mouse NSCs. Histogram of β-catenin Western blot signal intensities normalized to loading controls (n = 4).

    BMC Neurosci 2011 12, 50. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Morphological changes and histone H3 phosphorylation of HB cells treated with a combination of VX-680 and SAHA. HUH6 and HepT1 were incubated with VX-680 (6 μM) and SAHA (0.5 μM). Nuclei diameter (a) and cell diameter (b) were determined 72 h later by DAPI staining and microscopy. Data represent mean±SD of the diameters from 20 cells in each experiment. (* Two-way ANOVA, Bonferroni test, p \0.05). c Western blot analysis on HUH6 and HepT1 cells were carried out with an anti-phospho-Histone H3 (Ser 10) antibody (p-H3) 24 h after incubation with VX-680 (10 μM), SAHA (0.2 μM) or a combination of both. Controls were left untreated. Western blot analysis showed a decrease in p–H3 after treatment with VX-680 ( lane 2 ) relative to controls ( lane 1 ) and an increase when SAHA was added ( lane 3 ). For the combination of VX-680 and SAHA (lane 4 ) no p-H3 was detected.

    Pediatr Surg Int 2012 28, 579-89. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Vorinostat promotes C2C12 differentiation.In the image, the red is the myotube indicating differentiation ,the blue is the nuclei.

     

     

    Dr. Akihiko Yoshimura of Akihiko Yoshimura. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Western blot analysis of Acetyl-H3 and H3. 0-20μM SAHA was added.

     

     

    Dr. Zhang of Tianjin Medical University. Vorinostat (SAHA, MK0683) purchased from Selleck.

产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述 Vorinostat (suberoylanilide hydroxamic acid, SAHA)是一种HDAC抑制剂，无细胞试验中IC50为~10 nM。
特性 Vorinostat是广谱HDAC活性抑制剂，抑制I和II类酶 。
靶点
HDAC [1]
(Cell-free assay)
~10 nM
体外研究

Vorinostat是小分子量（<300）线性肟酸，抑制HDAC活性，包括乙酰化组蛋白和非组蛋白的积累，抑制培养细胞的增殖，且抑制肿瘤生长。Vorinostat通过结合到酶的活性位点而抑制 HDAC活性。Vorinostat 抑制多种转化细胞增殖（包括 淋巴瘤，多发性骨髓瘤，白血病，和非小细胞肺癌），与对照组相比抑制生长达50% 时的浓度为0.5 到10μM。Vorinostat作用于淋巴瘤和白血病细胞，包括 Burkitt, B-细胞急性淋巴细胞白血病 (B-ALL), MCL, DLBCL, ATL和T-细胞，不同程度抑制细胞增殖。除了抑制转化细胞增殖, Vorinostat 也抑制正常细胞增殖，通过比较Vorinostat 作用于一组细胞系–正常人非纤维细胞(WI-38)和 SV40巨大T抗原转化WI-38(VA-13细胞)而得到证明。Vorinostat 抑制两种类型细胞增殖，这种作用存在剂量依赖性。但发现Vorinostat 对转化细胞具有选择毒性，包括使肿瘤细胞死亡，而对正常细胞只起抑制作用，不会使其死亡。[1] Genistein 和Vorinostat 联用效果比5-aza和Vorinostat 联用作用于诱导细胞死亡有效很多。Genistein和Vorinostat联用作用于ARCaP-E使增殖降低 80 %，作用于ARCaP-M细胞，增殖降低60 %以上。Genistein和Vorinostat 联用抑制细胞增殖具有协同效应。作用于ARCaP-E 细胞(820个基因 诱导和1046个基因抑制) ARCaP-M 细胞，Vorinostat比Genistein 影响更多基因。[2] Vorinostat作用于肿瘤细胞表面，提高Fas蛋白水平，这种作用存在剂量依赖性，且按0.75 μM剂量处理，提高达到一个稳定水平。[3] Vorinostat 抑制肿瘤细胞生长，包括NB4, H460 和HCT-116，IC50分别为0.7μM, 3.4μM和1.2 μM。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A20 NELLTGRMcW6jc3WgRZN{[Xl? MmT4NUBp M{XETGROW09? M1TRcYFvfGlvSFTBR|Eh[WO2aY\peJkhf2m2aDDJR|UxKG:oIECuNVEzKM7:TR?= NIm4fpAyOTl4MES4PS=>
Friend leukemic cell MlXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW[1NEDPxE1? NVfYPFFIPzJiaB?= M{foSWROW09? NEXyXJhKSzVyPUCuPVkh|ryP Mn\4NVIyODl7MUO=
K562 NIXte5BMcW6jc3WgRZN{[Xl? NGPpSmE3KGh? NYe1co1xTE2VTx?= M3z2NGlvcGmkaYTpc44hd2ZiaIXtZY4hcGm|dH;u[UBl\WGlZYT5cIF{\SBqbXn4eJVz\SCxZjDISGFEOSCjbnSgTGRCSzJrIIfpeIghUUN3MDDv[kAxNjBzIN88US=> NV72fJNrOTJ2MUmzPFA>
HT1080 NVu0fYVFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWGySXJ[PzJiaB?= M4j1fGROW09? MUXJR|UxRTJwNDFOwG0> NV32[3VKOTJ{N{CxO|U>
H1299 NITJOYZMcW6jc3WgRZN{[Xl? NED4ZpUzOCCvaX6= NFvHVGNFVVOR MUXJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgTGRCSyCnbor5cYUhf2m2aDDJR|UxKG:oIECuNVk1KM7:TR?= NWnHO3BtOTR3MkG0NlI>
H1299 NE[3[HVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\TO|IhcA>? Mmq1SG1UVw>? NET3VGFKSzVyPUeuNlQh|ryP NVHYenN7OTR3MkG0NlI>
HeLa Mn3HT4lv[XOnIFHzd4F6 MXOzNEBucW5? MkDlTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiaHnzeI9v\SCmZXHj[ZR6dGG|ZTD3bZRpKEmFNUCgc4YhOC5{ODFOwG0> MW[xOVYxOzl2OR?=
LOX-IMVI M1nnNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIP1bmQ1QCCq NWrBWpRJUUN3ME2xMlMh|ryP MmSwNVU4OTV2N{C=
NCI-H226 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXFVVZvPDhiaB?= NVmyNXp2UUN3ME2yMlYh|ryP NWPOcoZKOTV5MUW0O|A>
RXF-631L MmSyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjPOFghcA>? MUnJR|UxRTJizszN NHK2cHoyPTdzNUS3NC=>
SNB-78 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTQcIw1QCCq MYTJR|UxRTF4IN88US=> NXrMSlZEOTV5MUW0O|A>
St-4 MkXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3;LS|Q5KGh? NFXP[mpKSzVyPUWuNkDPxE1? NYG3OmlOOTV5MUW0O|A>
ARP1 NF3uPG9HfW6ldHnvckBCe3OjeR?= NVvmeodLOiEQvF2= MlzwOkBp MXXJcoR2[3Srb36gc4YhWDJzV1HGNUBo\W6nIHX4dJJme3Orb36= NWDoenJYOTR5M{S4NFY>
CEM NEjMbZVMcW6jc3WgRZN{[Xl? NHXq[owyKGh? NIXGO4RFVVOR NUHxfGZCUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlM{KM7:TR?= MVyxOlQzODB4NB?=
HCT15 NVvsPXFwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVi0PEBp NYXYfJdoT0l3ME2wMlgzKM7:TR?= MkjJNVY4OjN{Mke=
NCI-H23 Mn;WS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmjHOFghcA>? MnK0S2k2OD1yLkmyJO69VQ>? MVyxOlczOzJ{Nx?=
GM15850 MnvhT4lv[XOnIFHzd4F6 NU\McmpTOi53IN88US=> M{DEXVk3KGh? M2HmeWROW09? NYTkc|d7UW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFMhdHm|aX7lJFE1KGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l NVHPdoxPOTZ7MkGzOlc>
GM15850 MVXLbY5ie2ViQYPzZZk> NVPpNmE4Oi53IN88US=> M37ZTFk3KGh? MUfEUXNQ M{TFdmlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFi0JIx6e2mwZTC1JIFk\XS7bHH0bY9vKG:oIF\YUkBo\W6n MWSxOlkzOTN4Nx?=
GM15850 NVvJXWNxU2mwYYPlJGF{e2G7 MmDCNk42KM7:TR?= MUK5OkBp MXvEUXNQ NXLoZ4x3UW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFEzKGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l M1ruclE3QTJzM{[3
GM15850 MlfjT4lv[XOnIFHzd4F6 Mne5Nk42KM7:TR?= NH;JZ3U6PiCq NVrOenRoTE2VTx?= MXzJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDsfZNqdmViOTDhZ4V1gWyjdHnvckBw\iCIWF6g[4Vv\Q>? MXSxOlkzOTN4Nx?=
GM15850 MkjHT4lv[XOnIFHzd4F6 NEnXU5gzNjVizszN MYO5OkBp NH3BWFdFVVOR MXTJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKNDDsfZNqdmViODDhZ4V1gWyjdHnvckBw\iCIWF6g[4Vv\Q>? NIfrc2syPjl{MUO2Oy=>
GM15850 NUL4OFJ6U2mwYYPlJGF{e2G7 NGLP[2wzNjVizszN NF23cGw6PiCq NYS3XVVSTE2VTx?= NGXWT2ZKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIOEBtgXOrbnWgNVYh[WOndInsZZRqd25ib3[gSnhPKGenbnW= MUGxOlkzOTN4Nx?=
U937 NYfYNZN[TnWwY4Tpc44hSXO|YYm= MY[1JO69VQ>? MlLuNlQhcA>? NHXXUVBFVVOR NVK3WWdDS2WubDDjfYNt\SCjcoLld5Qh[nliYXPjeY12dGG2aX;uJIF1KFNxR{KvUUBxcGG|ZR?= M{HRcFE4ODB2N{G4
H661 MmDsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW[0PEBp NEDmb2NKSzVyPUGg{txO NH:xfFYyPzF3N{CwPS=>
U937 MX7GeY5kfGmxbjDBd5NigQ>? NF7aVGs2KM7:TR?= MXyyOEBp NWTEUoVxTE2VTx?= MV\JcoNz\WG|ZTDpckBpcXO2b37lJGg{KGGlZYT5cIF1cW:w NH7KXFkyPzR2N{e1NC=>
U937 MYLGeY5kfGmxbjDBd5NigQ>? MmXKNUDPxE1? M4PIdlI1KGh? M4jQSGROW09? MYjF[oZm[3Rib36gZYxxcGFidIXieYxqdiCjY3X0fYxifGmxbh?= M{LCZlE4PDR5N{Ww
RAW264.7 MoTqSpVv[3Srb36gRZN{[Xl? NUXke|U2OTBizszN MWCyOIg> NX;ZPYV4f2G2ZYK= M1PtSWlvcGmkaYTpc44hd2ZiTGDTMZN1cW23bHH0[YQhXE6IYXzwbIEheHKxZIXjeIlwdiC5aYToJGlEPTBib3[gNE44PiEQvF2= MVmxO|Q4PzVzOB?=
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T24 M1nkRWtqdmG|ZTDBd5NigQ>? MXyzJIg> MWnJcoR2[3Srb36gc4YhcGm|dH;u[UBJOyCjY3X0fYxifGmxbjD3bZRpKEWFNUCgc4YhOC5zMjFOwG0> NGLqPY8yQTB6NEO5OS=>
T24 MUXLbY5ie2ViQYPzZZk> M{nVWFMhcA>? M4K3Smlv\HWldHnvckBw\iCjbIDoZUB1fWK3bHnuJIFk\XS7bHH0bY9vKHerdHigSWM2OCCxZjCwMlI2KM7:TR?= NV7NO5BTOTlyOESzPVU>
HeLa MmT5T4lv[XOnIFHzd4F6 NVniO5RWOTVibXnu M4f5R2ROW09? NFv4cWpKdmirYnn0bY9vKG:oIFjERWM3KG[{b32gcpVkdGWjcjDlfJRz[WO2IIfpeIghUUN3MDDv[kAxNjB6NUWg{txO NWXFVoxxOTlyOUO4PFQ>
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HeLa NHvYWHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\3[5lCPzJiaB?= MYfEUXNQ MnniTWM2OD1yLkS2JO69VQ>? NGDCO2kyQTR2MUi0Oi=>
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CFBE41o- M3LtXGtqdmG|ZTDBd5NigQ>? NX;zNmJrPSEQvF2= MnfsNlQhcA>? NE\3fnRFVVOR NHnDZ49KdmirYnn0bY9vKG:oIFjERWM4KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJJN1[WKrbHn0fUBw\iCIZHXseIE2ODhiQ1\UVkBxem:2ZXnu NIDPWGgyQTl4Nke4PS=>
CFBE41o- NUO2OHFCU2mwYYPlJGF{e2G7 MnHyOUDPxE1? MVmyOEBp NWHNWI42TE2VTx?= NFfGPYxKdmirYnn0bY9vKG:oIFjERWM4KGG|c3Xzd4VlKGG|IHPvdpJm[3Srb36gc4YhdXW2YX70JGZl\Wy2YUWwPEBETlSUIITyZYZncWOtaX7nJJRwKGOnbHygd5Vz\mGlZTDhd{BodHmlb3\vdo0> MlfsNVk6PjZ5OEm=
Primary bronchial epithelial cell MkDRT4lv[XOnIFHzd4F6 MnSwOUDPxE1? MWqyOEBp M2\wT2ROW09? MVjJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIH31eIFvfCCIZHXseIE2ODhiQ1\UVkBxem:2ZXnuJIFxcWOjbDDzeZJn[WOnIHzvZ4FtcXqjdHnvci=> NX3sNmtwOTl7Nk[3PFk>
Primary bronchial epithelial cell MonvT4lv[XOnIFHzd4F6 NVPvbo5oOSEQvF2= M3PQNlk3KGh? NEfSbo1FVVOR MoHTTY5pcWKrdHnvckBw\iCKRFHDO{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDzbI9zfCClaYLjeYl1KGO3coLlcpR{ Ml\aNVk6PjZ5OEm=
BxPC3 NVG3XWRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEC1cm84OiCq NYfD[G4zUUN3ME2yMlch|ryP NYrldGhZOjBzNEO3O|g>
CAL27 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4XtR|czKGh? NEL1TGlKSzVyPUOuO|Ih|ryP M3\qeFIxOTR|N{e4
Capan1 M2G2bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlXOO|IhcA>? MofOTWM2OD15LkOg{txO NXv3SIJvOjBzNEO3O|g>
HCC827 MlzoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXEbnI5PzJiaB?= NEHEW3JKSzVyPUGuPEDPxE1? MYKyNFE1Ozd5OB?=
LNCAP NVHXc2xIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkGxO|IhcA>? NGrUV4xKSzVyPUCuOVgh|ryP M2X5clIxOTR|N{e4
NCI-H2122 Ml21S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXG3NkBp MVTJR|UxRTdwNTFOwG0> MWKyNFE1Ozd5OB?=
NCI-H358 M2HoWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUm3NkBp MYDJR|UxRTJwNTFOwG0> NFLZOlgzODF2M{e3PC=>
SCC15 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4rOS|czKGh? NVvBbXIyUUN3ME2yMlQ6KM7:TR?= NWnSWHJiOjBzNEO3O|g>
SCC25 NWnkbppjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTqfWU4OiCq NVHIO3RTUUN3ME2xMlk{KM7:TR?= NWe5VZJ2OjBzNEO3O|g>
SKHEP1 MlzSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYC3NkBp NWPpSpRvUUN3ME2zMlQ3KM7:TR?= MoWxNlAyPDN5N{i=
BJ M373XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4[wXlExOCEQvF2= NEjlcI41QCCq MlvISG1UVw>? NGjtclFIem:5dHigbY5pcWKrdHnvckBifCByLkC1JJRwKDFyMDD1US=> MUKyNFE1Ozh2MB?=
MUF MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XzclExOCEQvF2= NVnwT2FoPDhiaB?= MVLEUXNQ MYfHdo94fGhiaX7obYJqfGmxbjDheEAxNjB3IITvJFExOCC3TR?= M1jyfVIxOTR|OESw
Caco-2 NFPCNJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWH1dnhpOTByIN88US=> MVS0PIg> MVLEUXNQ M4Poc2lEPTB;OD6xJO69VQ>? NHrGfpAzODF2M{i0NC=>
Caco-2 M3i1NmtqdmG|ZTDBd5NigQ>? NIC0NVc2KM7:TR?= NUXCS3I6PCCq MnjFSG1UVw>? MWXJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;uJGlEPTBib3[gNE4xOzdizszN NHn1dI0zODF2M{i0NC=>
Caco-2 NFvB[4ZMcW6jc3WgRZN{[Xl? MWKwMlMyPiCwTTD0c{A{OS54IN88US=> M{LnNVQhcA>? NH;SOXFFVVOR M1ziUGV5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTD3bZRpKEmFNUCgc4YhOC5zMUmg{txO NH73ZlMzODF2M{i0NC=>
Caco-2 MWHLbY5ie2ViQYPzZZk> MXuwMlMyPiCwTTD0c{A{OS54IN88US=> M3jKSVQhcA>? NGPueGdFVVOR NUDReHAxTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVOzJJdqfGhiSVO1NEBw\iByLkG0O{DPxE1? MoTrNlAyPDN6NEC=
Caco-2 MmTRT4lv[XOnIFHzd4F6 NHTmWFU3NjN{IH7NJJRwKDZ|MjFOwG0> NYnw[FhoPCCq Mlr5SG1UVw>? NGqwSohGgCC4aY\vJIlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Wge4l1cCCLQ{WwJI9nKDF{Lk[g{txO NE\1NYkzODF2M{i0NC=>
Caco-2 Mk\LT4lv[XOnIFHzd4F6 MnTKOk4{OiCwTTD0c{A3OzJizszN MUe0JIg> NE\pVW5FVVOR NV:1Ro9PTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO3JJdqfGhiSVO1NEBw\iB2MTFOwG0> NVPPXFltOjBzNEO4OFA>
Caco-2 M1\mTmtqdmG|ZTDBd5NigQ>? MoPLNE4{OTZibl2geI8hOzFwNjFOwG0> Ml3JOEBp MlHwSG1UVw>? M3LuUWV5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFNh?= M{j6SlIxOTR|OESw
HepG2 MVfLbY5ie2ViQYPzZZk> MkTZOUDPxE1? MoG2Oog> NF;LbWhFVVOR M3ewd2lv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgZYNmfHmuYYTpc44> NUnXcIJGOjBzNEO4OFA>
HepG2 NXfYfoJYU2mwYYPlJGF{e2G7 NFzmb5k2KM7:TR?= M2HUWVZp M1m0SGROW09? M1PGVGlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgbJlx\XKjY3X0fYxifGmxbh?= NELHXpQzODF2M{i0NC=>
HepG2 NUGzcZZDU2mwYYPlJGF{e2G7 MmG2OUDPxE1? MWS2bC=> MoTDSG1UVw>? NUS3[WtrUW6mdXP0bY9vKG:oIFjERWMudWWmaXH0[YQh[WyyaHGteJVjfWyrbjDhZ4V1gWyjdHnvci=> NGHXU2MzODF2M{i0NC=>
HepG2 NGLOTmtMcW6jc3WgRZN{[Xl? MYm1JO69VQ>? M4LRPVZp MWrEUXNQ MXXJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDIN{9JPCCqaYP0c45mKGGlZYT5cIF1cW:w Mo[0NlAyPDN6NEC=
COLO205 M3S5XGtqdmG|ZTDBd5NigQ>? NULOU|BtOTBizszN NF7LW3QzPCCq M3jrW2ROW09? Mof3TY5pcWKrdHnvckBw\iCKRFHDNUBie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gbIl{fG:wZTDIN{Bi[2W2eXzheIlwdiC5aYToJGlEPTBib3[gNk4zKM7:TR?= NWXlW3JiOjB2NUGzO|g>
SCC4 Mmq1T4lv[XOnIFHzd4F6 M{LtUFEh|ryP M3jLRlI1cA>? NEjqbodKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6geJVjfWyrbjDhZ4V1gWyjdHnvci=> MYmyNFQ2OjJ{NR?=
SCC4 M3\zWWtqdmG|ZTDBd5NigQ>? M2HodVEh|ryP NHLHW2EzPGh? M{SwdGlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIHHj[ZR6dGG2aX;u NVS0W2ppOjB2NUKyNlU>
SCC4 NGfD[otHfW6ldHnvckBCe3OjeR?= M4XQb|Eh|ryP NFjIUYQ2KG2rbh?= MYLB[49vcXO2IHHjeIl3cXS7IHH0JHZFWiCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhS1mSMkSg[4Vv\SCneIDy[ZN{cW:w NVfs[3FUOjB2NUKyNlU>
NB4 MUPGeY5kfGmxbjDBd5NigQ>? NEHFcFk2KM7:TR?= M3rtS|I1KGh? MnLSSG1UVw>? M3\wcGNmdGxiY4njcIUh[XK{ZYP0JIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHHjZ5VufWyjdHnvckBifCCycnWtS|EheGijc3W= NV;X[2poOjB2OUG0OFA>
NB4 MkS5T4lv[XOnIFHzd4F6 Mk\1OUDPxE1? NYHBVmtSOjRiaB?= MoHOSG1UVw>? NGLKbWpKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZYNmfHmuYYTlJJR2[nWuaX6gcIV3\Wx? MX2yNFQ6OTR2MB?=
NB4 M2eyWmZ2dmO2aX;uJGF{e2G7 MVK1JO69VQ>? NFTwZmczPCCq M4TheGROW09? NG[4emxKdmS3Y4Tpc44hd2ZicEKxW2FHOS:FSWCxJIV5eHKnc4Ppc44> M3WwS|IxPDlzNESw
NB4 MVnLbY5ie2ViQYPzZZk> MnjmOUDPxE1? M37nPFI1KGh? NH\XZ|ZFVVOR NYT0TXRUUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFMh[WOndInsZZRqd25? M1jsOFIxPDlzNESw
NUGC3 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEGzbXdIUTVyPUKuPVQh|ryP M2nEblIxQDVyOUex
A549 NFTaSIhHfW6ldHnvckBCe3OjeR?= NV3iVJVqPSEQvF2= NYfGTGVrOyCq NXS2VnY3TE2VTx?= M2L4b2lv\HWldHnvckBw\iCjdYTvdIhi\3liaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCOQ{PJTS=> NIDocpEzODh3NUKwPC=>
HUT78 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWK3NkBp MlvoSG1UVw>? MWTHTVUxRTBwMzFOwG0> NGPtT40zODh6NEKwPC=>
U937 M1nsVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEP6VGQ4OiCq M1Hv[GROW09? MWPHTVUxRTBwM{mg{txO NUeySlNLOjB6OESyNFg>
A431 MXTDfZRwfG:6aXOgRZN{[Xl? NFHab3U4OiCq Ml;pR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVQ{OSClZXzsd{BwfmW{ZYjwdoV{e2mwZzDFS2ZTKHerdHigTWM2OCCxZjC0MlIh|ryP NW\KXW16OjFyOEC2Nlk>
A549 NVTCNIQ5S3m2b4TvfIlkKEG|c3H5 MoXlO|IhcA>? NFPWSGxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJGhFSUNid3n0bEBKSzVyIH;mJFEvQCEQvF2= NFi4N5ozOTB6ME[yPS=>
CAL27 NGPKPVhEgXSxdH;4bYMhSXO|YYm= NH\XeW84OiCq MkfaR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2FNOjdiY3XscJMhd3[ncnX4dJJme3OrbnegSWdHWiC5aYToJGlEPTBib3[gN{4zKM7:TR?= MWCyNVA5ODZ{OR?=
HeLa MYXDfZRwfG:6aXOgRZN{[Xl? NWLpPHNQPzJiaB?= MorsR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISGFEKHerdHigTWM2OCCxZjCxMlUh|ryP NWThZmg6OjFyOEC2Nlk>
Sf21 MlrhT4lv[XOnIFHzd4F6 NH25OGw{KGh? NFj1VIpKdmirYnn0bY9vKG:oIF\sZYchfGGpZ3XkJIh2dWGwIILlZ49u[mmwYX70JGhFSUN6IHX4dJJme3OnZDD3bZRpKEmFNUCgc4YhOS5{IN88US=> MVqyNVA5ODZ{OR?=
SKBR3 NELnc3dEgXSxdH;4bYMhSXO|YYm= NVfjU4pIPzJiaB?= NX;hUXdkS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uEUkOgZ4VtdHNib4\ldoV5eHKnc4PpcochUEWUMjD3bZRpKEmFNUCgc4YhOi54IN88US=> MWiyNVA5ODZ{OR?=
SKOV3 M2OyZmN6fG:2b4jpZ{BCe3OjeR?= NFiydYc4OiCq MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISXIzKHerdHigTWM2OCCxZjCyMlIh|ryP NHfJWoIzOTB6ME[yPS=>
COR-L23 NGftbI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTqNVAh|ryP M4Pjd|czKGh? MkLXSG1UVw>? MV\HTVUxRTFwMTFOwG0> MYWyNVA5ODZ2Nx?=
DU145 MojJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3PLOlExKM7:TR?= MWq3NkBp MmnSSG1UVw>? M4LlbWdKPTB;MT6wOUDPxE1? M3HRe|IyODhyNkS3
LoVo NUX6PVE4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH\4[XcyOCEQvF2= MUi3NkBp NXjhSW1kTE2VTx?= MYXHTVUxRTFwNzFOwG0> NF;R[5YzOTB6ME[0Oy=>
MCR5-SV2 NVPiZWw1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVLJ[4FVOTBizszN NGLkRpg4OiCq NHm2UnJFVVOR NVnINFZCT0l3ME2yMlEh|ryP MYqyNVA5ODZ2Nx?=
HCT116 NFrjNHpMcW6jc3WgRZN{[Xl? NH;ndGQ{OCEQvF2= MW[zNEBp NV\RZ2t{TE2VTx?= M3e3TGlvcGmkaYTpc44hd2ZiSFTBR|Yh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFteGijLYT1ZpVtcW5iYXPleJlt[XSrb36= MmHuNlExQDB4NEe=
HCT116 NWW4XGpHU2mwYYPlJGF{e2G7 NWrSNJpROzBizszN NH3Cfms{OCCq NWrXXYlpTE2VTx?= NWPyd|NMUW6qaXLpeIlwdiCxZjDISGFEOSCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhcGm|dH;u[UBJO0t7IHHj[ZR6dGG2aX;u M4exWVIyODhyNkS3
U937 M2LTWmtqdmG|ZTDBd5NigQ>? M3G3S|Uh|ryP MXeyOEBp NW\yU|FJTE2VTx?= M1SwdmlvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDv[kBi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KEt7 Mln4NlEzQTJ2OUK=
U937 NVK5UoRbU2mwYYPlJGF{e2G7 NIPMXnE2KM7:TR?= M3PiRlI1KGh? NXTTd3ZWTE2VTx?= MUHJcohq[mm2aX;uJI9nKGirc4TvcoUh[WOndInseJJidnOoZYLhd4Uh[XO|ZYPz[YQh[XNiaX7jdoVie2Vib3[gZYNmfHmuYYTpc44hd2ZiaHnzeI9v\SCKMzDheEBMOTh? NUTISVZzOjF{OUK0PVI>
U937 M3nvUGtqdmG|ZTDBd5NigQ>? NV\neZRSPSEQvF2= M1H5OlI1KGh? MlPOSG1UVw>? MVTJcohq[mm2aX;uJI9nKGirc4TvcoUh[WOndInseJJidnOoZYLhd4Uh[XO|ZYPz[YQh[XNiaX7jdoVie2Vib3[gdIFvNWGlZYT5cIF1\WRiaHnzeI9v\SCKNDDs[ZZmdA>? MkfWNlEzQTJ2OUK=
U937 MkG0T4lv[XOnIFHzd4F6 MmjrOUDPxE1? NISyfXUyPSCvaX6= MXrEUXNQ M4DDbGlvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDv[kBpcXO2b37lJGg{KGGlZYT5cIF1cW:wIHzleoVt M2XhS|IyOjl{NEmy
A2780 NFS5ZZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIqzV4E6PiCq MnHESG1UVw>? MkXOTWM2OD1zLk[yJO69VQ>? NIXvVZQzOTZ|NESzNC=>
COLO205 NHLaVZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3\WdVk3KGh? NHLEUHlFVVOR NVvrbnVrUUN3ME2yMlEzKM7:TR?= NFHTc5AzOTZ|NESzNC=>
Hep3B NX\4WHYzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnP1OFghcA>? NYHwOlVGTE2VTx?= NYC5SFBmT0l3ME2xMlIyKM7:TR?= MWqyNVcyOjF2Nh?=
GM11272 MVrGeY5kfGmxbjDBd5NigQ>? MkjZNVAxKG6P NIfxSnk4OiCq MlPuSG1UVw>? MVXS[YFkfGm4YYTpc44hd2ZiTXXDdFIhdXW2YX70JIV5eHKnc4Ppc44h[XRiMUCgeI8hOTByIH7N NFPGflQzOTh2MEexOi=>
CFBE41o M{THb2tqdmG|ZTDBd5NigQ>? M2fsXFUh|ryP MoPZSG1UVw>? M4DBTGRwf25icnXneYxifGmxbjDv[kBJTEGFNzDheEAxNjJidH:gOUB2VQ>? M{TXUlIyQTh2OUW4
CFBE41o MYTGeY5kfGmxbjDBd5NigQ>? M3zzflEh|ryP MUSyOEBp MUDEUXNQ NGjhbXBT\XO2b4LheIlwdiCxZjDDSnRTKGSnbIThSlUxQCCvdYThcpQhfHKjZn\pZ4tqdmdidH:gZ4VtdCC|dYLmZYNmKGG|IHfsfYNw\m:{bYO= M13lWlIyQTh2OUW4
DU145 NHHtcVdMcW6jc3WgRZN{[Xl? MXeyMlUh|ryP M1XMXVI1KGh? M{O0WWROW09? MWHJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhfXC{ZXf1cIF1cW:wIH;mJJAzOXejZkGgdJJwfGWrbjDlfJBz\XO|aX;u NVi5[IFOOjJ{NkCxOlY>
DU145 M4LyWGtqdmG|ZTDBd5NigQ>? MYm1JO69VQ>? M{\HbVI1KGh? MWXEUXNQ NEWyU|FKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaHnzeI9v\SCKNDDhZ4V1gWyjdHnvci=> Mn;FNlIzPjBzNk[=
DU145 NYnQdVdWU2mwYYPlJGF{e2G7 MXm1JO69VQ>? M1rFSFI1KGh? MknuSG1UVw>? Mo\mTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGirc4TvcoUhUDNiaInw[ZJi[2W2eXzheIlwdg>? M3r0OlIzOjZyMU[2
786-0 MkXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX3XTHUxPi5{IN88US=> MXi3NkBp MV3EUXNQ NGfUdI9IUTVyPUOuNkDPxE1? M{LmfFIzPDN3Nk[5
A498 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXO2MlIh|ryP MnLmO|IhcA>? M4X0R2ROW09? MkmxS2k2OD1zLk[g{txO MmPaNlI1OzV4Nkm=
A549/ATCC Ml3rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFP5SZY3NjJizszN M2fke|czKGh? MY\EUXNQ M{[1PGdKPTB;MT62JO69VQ>? NIHPTWIzOjR|NU[2PS=>
ACHN MkP0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVu2MlIh|ryP NX3YfpN4PzJiaB?= NWHMeGlYTE2VTx?= M2rqSWdKPTB;MT6zJO69VQ>? MoHLNlI1OzV4Nkm=
BT549 NG\J[3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDMV3NqPi5{IN88US=> M1TTRlczKGh? MkPVSG1UVw>? MXTHTVUxRTFwMzFOwG0> NHm4ZmMzOjR|NU[2PS=>
EKVX Mk\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn62Ok4zKM7:TR?= MVe3NkBp MUDEUXNQ M2jJWGdKPTB;MT6zJO69VQ>? NFG4bFAzOjR|NU[2PS=>
HCT15 MmfjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\GOk4zKM7:TR?= MV:3NkBp MWHEUXNQ MkfFS2k2OD1{LkWg{txO MXGyNlQ{PTZ4OR?=
HOP92 MkHzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvmV3Q3NjJizszN NHvoc|A4OiCq M4K0W2ROW09? M16wVGdKPTB;NDFOwG0> M1PNcFIzPDN3Nk[5
IGROV1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnzlOk4zKM7:TR?= NYrqPW1kPzJiaB?= MWrEUXNQ M4q2SmdKPTB;MT6zJO69VQ>? NV7OTlAyOjJ2M{W2Olk>
LOXIMVI MmnTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HkOVYvOiEQvF2= NHz4Sog4OiCq NUXnfI91TE2VTx?= NFjPeXZIUTVyPUGg{txO NFmxb2IzOjR|NU[2PS=>
M14 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWi2MlIh|ryP M3vsSFczKGh? MlL2SG1UVw>? Mme1S2k2OD1zLkOg{txO NVP0fodkOjJ2M{W2Olk>
MALME-3M NGLGTXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jXOVYvOiEQvF2= NEfOTGg4OiCq MVnEUXNQ MUfHTVUxRTBwM{Kg{txO NWTjWlNYOjJ2M{W2Olk>
MCF10A MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2HKblYvOiEQvF2= NH7W[Gc1QCCq M{PTV2ROW09? M2nOcGdKPTB;OT62JO69VQ>? NIfkOZMzOjR|NU[2PS=>
MDA-MB-435 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDCSVRGPi5{IN88US=> NGXzboI4OiCq MlzUSG1UVw>? NWrBbZF{T0l3ME2wMlUh|ryP MkLMNlI1OzV4Nkm=
MDA-MB-468 M{SzVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUK2MlIh|ryP NVPieopYPzJiaB?= MVvEUXNQ NHP1[FlIUTVyPUCuOlMh|ryP M3r5WlIzPDN3Nk[5
MOLT4 Mkj5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUe2MlIh|ryP MknzO|IhcA>? NHn5RmNFVVOR NV;EZpdET0l3ME2wMlQh|ryP Mlf6NlI1OzV4Nkm=
NCI-ADR-RES NEmzPYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXzOk4zKM7:TR?= MWS3NkBp MmXhSG1UVw>? M4HPWWdKPTB;MD6xOkDPxE1? MlnSNlI1OzV4Nkm=
NCI-H23 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mm\HOk4zKM7:TR?= MVK3NkBp MkXLSG1UVw>? NXnQW25pT0l3ME2xJO69VQ>? NH;rPIkzOjR|NU[2PS=>
NCI-H322M M1HKXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHuTXZMPi5{IN88US=> M37zWlczKGh? NVTuOokzTE2VTx?= NWq2Xnp6T0l3ME2wMlc6KM7:TR?= MXqyNlQ{PTZ4OR?=
NCI-H460 NFfkOFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\MR2I3NjJizszN MX[3NkBp NFSyeJdFVVOR NHeyS5VIUTVyPUCuO|kh|ryP M3XONFIzPDN3Nk[5
NCI-H522 MoLpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlq4Ok4zKM7:TR?= MUi3NkBp MojzSG1UVw>? NFraXoFIUTVyPUCuOUDPxE1? NH7zd2YzOjR|NU[2PS=>
OVCAR3 NGrEd3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2T3XlYvOiEQvF2= NV7HeHJ6PzJiaB?= M4PueGROW09? MWXHTVUxRTFwMzFOwG0> M3fCeVIzPDN3Nk[5
OVCAR4 M{fwXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX:2MlIh|ryP MW[3NkBp M2nSNGROW09? M1K4[2dKPTB;NDFOwG0> MX2yNlQ{PTZ4OR?=
OVCAR5 Mmm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYS2MlIh|ryP M4HnelczKGh? MoK3SG1UVw>? NEjnZ5JIUTVyPUCuO|kh|ryP MYmyNlQ{PTZ4OR?=
OVCAR8 M3n2UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvXOk4zKM7:TR?= NXX2ZmlNPzJiaB?= MnLMSG1UVw>? MWfHTVUxRTBwNTFOwG0> NYTaW3IyOjJ2M{W2Olk>
RXF393 NVjBZllWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnrLOk4zKM7:TR?= M1WxeVczKGh? MY\EUXNQ M{[2WWdKPTB;MT6zJO69VQ>? MV[yNlQ{PTZ4OR?=
SF268 NV\xSppRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYm2MlIh|ryP MnXKO|IhcA>? NF3ic3pFVVOR NXzJNHlZT0l3ME2xMlYh|ryP MnH5NlI1OzV4Nkm=
SF295 NUnreotFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjHSINsPi5{IN88US=> NWO4cFJFPzJiaB?= NXqwZml5TE2VTx?= NXn4b3NTT0l3ME2xMlYh|ryP NInBPYgzOjR|NU[2PS=>
SF539 NU\JPJpqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYm2MlIh|ryP NEXlfmk4OiCq M3v1cWROW09? MX\HTVUxRTJizszN NED3UI4zOjR|NU[2PS=>
SK-MEL-2 NV3GNYZzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPGOk4zKM7:TR?= MYq3NkBp M3rwTWROW09? NYTWfGp2T0l3ME2xMlMh|ryP NYrZSZJZOjJ2M{W2Olk>
SK-MEL-28 MnjNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV62MlIh|ryP NIHw[VE4OiCq NUDqeWg5TE2VTx?= MYnHTVUxRTFizszN NEPwbI8zOjR|NU[2PS=>
SK-MEL-5 M1myR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2fnSlYvOiEQvF2= Mkj6O|IhcA>? MoPZSG1UVw>? MlT1S2k2OD1yLk[zJO69VQ>? MUOyNlQ{PTZ4OR?=
SKOV3 NFPRdpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWe1ZYJNPi5{IN88US=> MoS0O|IhcA>? MmPLSG1UVw>? MWjHTVUxRTFizszN MnvnNlI1OzV4Nkm=
SN12C M{LzRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXTkSVFnPi5{IN88US=> MV23NkBp MYjEUXNQ Mly0S2k2OD1{IN88US=> MX[yNlQ{PTZ4OR?=
SNB19 NFj1PXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXGycYxYPi5{IN88US=> MXe3NkBp M17DOGROW09? NHHQN3NIUTVyPUCuOlMh|ryP MmLYNlI1OzV4Nkm=
SNB75 NITYUWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWm2MlIh|ryP M1nlRVczKGh? MV;EUXNQ MmjLS2k2OD1yLke5JO69VQ>? M{\1ZVIzPDN3Nk[5
SR MmLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXu2MlIh|ryP MYS3NkBp NHLqNXhFVVOR M4XObGdKPTB;MD60JO69VQ>? NH;qUIMzOjR|NU[2PS=>
T47D M4HJNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXO2MlIh|ryP NWfXTXZSPzJiaB?= NITiSoxFVVOR MYrHTVUxRTBwNTFOwG0> MXWyNlQ{PTZ4OR?=
TK10 NGDUVXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUe2MlIh|ryP MlPMO|IhcA>? NGnNbZFFVVOR NEXQZotIUTVyPUCuOlMh|ryP NIP2dIUzOjR|NU[2PS=>
UACC257 M361O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVvwT|JxPi5{IN88US=> MYq3NkBp NWHMe3FqTE2VTx?= MluxS2k2OD1yLkWg{txO M3;NTVIzPDN3Nk[5
UACC62 NEXvfo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfG[olUPi5{IN88US=> MmHKO|IhcA>? MoXjSG1UVw>? M2T1d2dKPTB;MD60JO69VQ>? NWC1fI82OjJ2M{W2Olk>
UO31 MmHQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXG2MlIh|ryP M2\LUVczKGh? NWf6bXhNTE2VTx?= NIPBNnlIUTVyPUCuOUDPxE1? NYTGeW41OjJ2M{W2Olk>
Hep3B NH\FNIpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX7XN4pQPDhiaB?= NUHaRXZuTE2VTx?= MVPHTVUxRTBwNkmg{txO MlLPNlI1Ozl6NkO=
BE(2)-C MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV[3NkBp NFzKZYhFVVOR M3vWWWlEPzB;MjFOwG0> M1n0bVIzQTN{M{G2
Bel7402 M3TNN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWW3NkBp MnTKSG1UVw>? MYLHTVkxRTF5IN88US=> MVuyN|A3OTN5Nh?=
Bel7404 M1fRSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1zjflczKGh? MWHEUXNQ NXzCfVl3T0l7ME2xPEDPxE1? MkXyNlMxPjF|N{[=
BGC823 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlnrO|IhcA>? Mn;rSG1UVw>? NXfjVHpOT0l7ME23MlUh|ryP Ml3ZNlMxPjF|N{[=
MGC803 NET5Uo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2q0XFczKGh? M3;IV2ROW09? MojsS2k6OD1zMD64JO69VQ>? NXTJeHF7OjNyNkGzO|Y>
SGC7901 NIfSUFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUnt[pRqPzJiaB?= MnzvSG1UVw>? MYfHTVkxRTlwNDFOwG0> M131PVI{ODZzM{e2
SKHEP1 NHjZfWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TlUlczKGh? NIO3SZlFVVOR MW\HTVkxRTF5IN88US=> NWm3WJFJOjNyNkGzO|Y>
SMMC7721 M1nMOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXu3NkBp M3HqeGROW09? MXjHTVkxRTJyIN88US=> NWTqdpZyOjNyNkGzO|Y>
NUGC3 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\KNVAh|ryP NIDDTHBIUT1{Lkm0JO69VQ>? NV2wcVJvOjNzNkOzN|I>
A2780 CisR NVXLVZBIU2mwYYPlJGF{e2G7 NEXXfYsyQCCq NGT0WJRKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOFQh|ryP NEC1UpkzOzJ3Mk[wNy=>
CAL27 sens NFG1XVZMcW6jc3WgRZN{[Xl? NH\rS4wyQCCq NIK1[mpKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuN|Yh|ryP Ml2xNlMzPTJ4MEO=
CAL27 CisR NUG3enk4U2mwYYPlJGF{e2G7 NF;Q[FYyQCCq NITSPXBKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOlEh|ryP NWriTGlPOjN{NUK2NFM>
KYSE-510 CisR NUThSJpXU2mwYYPlJGF{e2G7 NEDH[Y0yQCCq NHfYcWlKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOVkh|ryP MXGyN|I2OjZyMx?=
KYSE-510 sens M1[0fWtqdmG|ZTDBd5NigQ>? NYrUSXhGOThiaB?= NGf4dFRKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuO{DPxE1? MmSyNlMzPTJ4MEO=
MDA-MB-231 sens NEDPfXdMcW6jc3WgRZN{[Xl? M1n3XFE5KGh? M3HnUmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD62NUDPxE1? MUSyN|I2OjZyMx?=
MDA-MB-231 CisR NGDsS4dMcW6jc3WgRZN{[Xl? MUWxPEBp MYHJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNkGg{txO NF\QR3QzOzJ3Mk[wNy=>
ES2 Ml;oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWf4RWpvPDhiaB?= M{jmUGROW09? NX;iXVU1UUN3ME2xNk44KM7:TR?= NYS2OppPOjN2OUO0OFk>
MDA-MB-231 NGq3WoxMcW6jc3WgRZN{[Xl? NETSTG0yOCEQvF2= MXezNEBucW5? M{S0eGROW09? MXnJcohq[mm2aX;uJI9nKEiGQVOyZS=> M3q4XFI{PDl|NES5
MDA-MB-231 M1HKcWtqdmG|ZTDBd5NigQ>? MnL1NUDPxE1? NYLoNZByOjRiaB?= MV3EUXNQ M1TkOGlvcGmkaYTpc44hd2ZiSFTBR|Yh[XO|ZYPz[YQh[XNiYXPleJlt[XSrb36gcIV3\Wy|IH;mJJR2[nWuaX6= M3zWUFI{PDl|NES5
HS68 MXTDfZRwfG:6aXOgRZN{[Xl? NIHmdZcyOCEQvF2= M1HSO|czKGh? NHe3VGFFVVOR MVfJR|UxRTRwNjFOwG0> M1HZUFI{PTdyNUSy
MEF NVPLTHNYS3m2b4TvfIlkKEG|c3H5 NF;oOGcyOCEQvF2= MV[3NkBp MUHEUXNQ NVLrRVNPUUN3ME20MlQh|ryP NV34clZ{OjN3N{C1OFI>
BGC823 NHfzV2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{XqfGROW09? Mnr5S2k2OD1|LkC5JO69VQ>? NYDWU49tOjN4MEG3NFY>
High5 M{Hl[mtqdmG|ZTDBd5NigQ>? NH3JXY4zPCCq NIj3OlhKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWM3KHerdHigTWM2OCCxZjCwMlA6KM7:TR?= NGfKbG8zOzZyMkWyNy=>
High5 M{K2VmtqdmG|ZTDBd5NigQ>? M2DsU|I1KGh? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOxJJdqfGhiSVO1NEBw\iByLkGyJO69VQ>? M3u4PFI{PjB{NUKz
High5 MWXLbY5ie2ViQYPzZZk> M2X4VlI1KGh? NGS5[mpKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWM{KHerdHigTWM2OCCxZjCwMlE4KM7:TR?= MXSyN|YxOjV{Mx?=
A549 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nTd2ROW09? M2PXTWlEPTB;MT6wO|kh|ryP NUjpUHBKOjN4NESyNVA>
HCT116 NI[3NWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHmTItFVVOR MWfJR|UxRTBwN{W3JO69VQ>? Mn7kNlM3PDR{MUC=
K562 M1HYTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn75SG1UVw>? M1LmS2lEPTB;MD62OFUh|ryP M3LDOlI{PjR2MkGw
GBC-SD M4H0WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fVd|Uh|ryP M3jtV|czKGh? NXvRWJBOTE2VTx?= MkHwTWM2OD1zND64JO69VQ>? NHfLdpAzOzdyN{K2NC=>
HGC27 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIXONYY2KM7:TR?= M3GxVlczKGh? MmrUSG1UVw>? MVrJR|UxRTVwNDFOwG0> NFnzSYwzOzdyN{K2NC=>
HL60 NGT0OpJMcW6jc3WgRZN{[Xl? NXr2bGVJPSEQvF2= MnvWNlQhcA>? MnjBSG1UVw>? M1PWSGlvcGmkaYTpc44hd2ZiSFTBRy=> M3nsU|I{PzB5Mk[w
Vero MlLWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYnyXItwPzJiaB?= M17ZRmROW09? NVW2UlhVUUN3ME2wMlkh|ryP MUKyN|c5PjR3Mh?=
NCI60 NFvkSmNEgXSxdH;4bYMhSXO|YYm= MmDxOFghcA>? NIfab|BFVVOR NGXXbotIUT1yLkWzJO69VQ>? M3vhcVI1ODF3M{K3
SW620 NYrtUGx[S3m2b4TvfIlkKEG|c3H5 NYfjOmtwPDhiaB?= MlTUSG1UVw>? NVHCOFlVT0l;MD61OEDPxE1? MVKyOFAyPTN{Nx?=
U251 MnTiR5l1d3SxeHnjJGF{e2G7 MmH2OFghcA>? NIq3blJFVVOR MnXRS2k:OS53MzFOwG0> NWm2b4plOjRyMUWzNlc>
KM12 NGTNW|hEgXSxdH;4bYMhSXO|YYm= M1vsU|Q5KGh? MYHEUXNQ MnH4S2k:OS56ODFOwG0> M{fNXlI1ODF3M{K3
Hs578T M1nyb2N6fG:2b4jpZ{BCe3OjeR?= M4G3UVQ5KGh? Mk\ZSG1UVw>? MWLHTV01Njh|IN88US=> MoO2NlQxOTV|Mke=
MDA-MB-231 NE\se|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFGyUpg1QCCq MWrJR|UxRTBwMU[g{txO NWnBRYJjOjRyOUWwNVY>
MCF7 NGfsNIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUO0PEBp NVjCenF{T0l;MD60OUDPxE1? NYXJVGZqOjRzMUm1OVU>
MDA-MB-435 NF;VRW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHZNWRzPDhiaB?= MXfHTV0xNjh6IN88US=> M2r3TFI1OTF7NUW1
AsPC1 MmrCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF;IWoU{OCEQvF2= NWHY[VlLPDhiaB?= M33oN2ROW09? M4Lpc2lEPTB;Mz62OkDPxE1? MUiyOFE5PTN5OB?=
Jurkat E6.1 NGfkcWhMcW6jc3WgRZN{[Xl? MVyyJIg> NH;wT2lFVVOR M2HmcWlvcGmkaYTpc44hd2ZiSFTBR|Qhf2m2aDDJR|UxKG:oIECuNFQh|ryP M3mzNFI1OjZzOE[y
KG1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17OeVQ5KGh? NWHxcGF3UUN3ME2xMlE2KM7:TR?= MmDhNlQ2OjVyMEO=
HEL NILr[XJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzhb3Q1QCCq NIT6Z|dFVVOR NUDLPWFiUUN3ME2wMlQ6KM7:TR?= NFu2NogzPDZ7NEC1OS=>
HSC3 MVPDfZRwfG:6aXOgRZN{[Xl? NWezUoFCOTJiaB?= NGruTY9FVVOR MXrJR|UxRTBwN{Gg{txO MUGyOFc3PjV4MB?=
HT-29 NYXJbGo{S3m2b4TvfIlkKEG|c3H5 NVL4NIJvOTJiaB?= MoTKSG1UVw>? NG\RSGxKSzVyPUCuO|Ih|ryP M4\FXlI1PzZ4NU[w
KB MnjkR5l1d3SxeHnjJGF{e2G7 MVOxNkBp M1;ye2ROW09? NX3WcIF2UUN3ME2wMlc2KM7:TR?= MVyyOFc3PjV4MB?=
H460 NUHaUFl{S3m2b4TvfIlkKEG|c3H5 M{nwPVEzKGh? M3z4SWROW09? M13wU2lEPTB;MD64NkDPxE1? MljmNlQ4PjZ3NkC=
MONE1 MmewR5l1d3SxeHnjJGF{e2G7 MVmxNkBp MUnEUXNQ MkXsTWM2OD1zLkG4JO69VQ>? NX7sNVhDOjR5Nk[1OlA>
MKN45 MoWzR5l1d3SxeHnjJGF{e2G7 NVTaVpREOTJiaB?= NYLkUIJ5TE2VTx?= MVzJR|UxRTFwMkWg{txO MoizNlQ4PjZ3NkC=
TSGH MkLNR5l1d3SxeHnjJGF{e2G7 M{X0ZlEzKGh? M2TBXWROW09? M4jHVGlEPTB;Mj6wPUDPxE1? NH;pO2wzPDd4NkW2NC=>
HL60 NYjJW5ZXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWPxRWFOPzJiaB?= M2fDSGROW09? NVPWZ3FIT0l3ME2wMlk{KM7:TR?= M2L4d|I1QTByNUCw
HEK293 NUHscHptT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrRZ3Y4OiCq M1z1V2ROW09? NGLPeVdIUTVyPUGzJO69VQ>? NGLtb5UzPDlyMEWwNC=>
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HT NX3O[FJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWXOPJpwUUN3ME2xMlg5QTR5IN88US=> NVizZWY3W0GQR1XS
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CGTH-W-1 MkC1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTFwOEm5OFYh|ryP NX7qZ4VKW0GQR1XS
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human HOP-92 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzDO2l4UUN3ME2xMlk1ODZ3IN88US=> NET3UlhUSU6JRWK=
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LB2241-RCC M4OxW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnWzTWM2OD1zLkm4NlMyKM7:TR?= NYjJVYpjW0GQR1XS
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CAL-51 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTJwMU[2PVkh|ryP M1rDOXNCVkeHUh?=
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GB-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvOOYpuUUN3ME2yMlMzOzN3IN88US=> NWroW5ZlW0GQR1XS
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RH-18 NXnIN2NyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nlVmlEPTB;Mj6zOVAzOiEQvF2= NHrmUYlUSU6JRWK=
NCI-H1155 M1PGOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFXiVZpKSzVyPUKuN|Y4OjVizszN MonFV2FPT0WU
NB17 M4[zWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTJwM{iyOFMh|ryP NHnobJlUSU6JRWK=
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KLE NFfnbYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFGyeIJKSzVyPUmuN|IzOzJizszN Mm\vV2FPT0WU
Calu-6 Mmf5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTlwM{e1Nlgh|ryP M{HrUnNCVkeHUh?=
LB831-BLC MnLhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlH6TWM2OD17LkS1NVcyKM7:TR?= Mln2V2FPT0WU
SW684 NHjyU2lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXH1OGVKUUN3ME25MlQ3OTF4IN88US=> MVLTRW5ITVJ?
SH-4 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEHnbo1KSzVyPUmuOFk{PjRizszN MV7TRW5ITVJ?
IST-MES1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLGWWpGUUN3ME25MlUyPjR2IN88US=> MmjzV2FPT0WU
Saos-2 NFXNfYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVH0[HV2UUN3ME25MlUzOTl3IN88US=> NWDhZ25FW0GQR1XS
NCI-H2087 M4PkbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTlwNkezOVIh|ryP NH7uTodUSU6JRWK=
SCC-9 NIrPemRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTlwN{G4NVYh|ryP NHXhT|hUSU6JRWK=
MFE-280 MlPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHTWUnBKSzVyPUmuO|I4QDVizszN MmL0V2FPT0WU
BEN NF[1XW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrrTWhKSzVyPUmuPFIyPTlizszN M3rYcXNCVkeHUh?=
CP50-MEL-B Ml[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPjTWM2OD17LkmyOFMzKM7:TR?= NFTmSVBUSU6JRWK=
SK-MEL-28 MlL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4LtPGlEPTB;MUCuNFE6OyEQvF2= NHP1NHdUSU6JRWK=
NCI-H2452 M1zodGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXv5[Hd4UUN3ME2xNE4yPiEQvF2= NHP0SnNUSU6JRWK=
NCI-H1395 NXrQSYh6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTFyLkG3PEDPxE1? NGLRW|FUSU6JRWK=
NCI-H2052 MoPJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXwTWM2OD1zMD6xPFgzKM7:TR?= MVTTRW5ITVJ?
A375 NXzUWGZ[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF7YdGpKSzVyPUGwMlI1PTJizszN MmjIV2FPT0WU
HCC38 NW\OPINvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3ZenVVUUN3ME2xNE4{OjJizszN MVPTRW5ITVJ?
LK-2 MmTMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M125NGlEPTB;MUCuOFIxQCEQvF2= M2TW[HNCVkeHUh?=
SF126 Mm\mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{nDZ2lEPTB;MUCuOFczPyEQvF2= NFL5VHNUSU6JRWK=
SK-MEL-1 NWLINHY2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHqTWM2OD1zMD63PFM5KM7:TR?= MojhV2FPT0WU
ACN MkXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVO2XIdjUUN3ME2xNE45Pjd7IN88US=> MkezV2FPT0WU
BB30-HNC MnrpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTFyLki3NVEh|ryP MVvTRW5ITVJ?
NCI-H747 NYPjTJRRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\aVmlEPTB;MUCuPFc1PiEQvF2= NYnncGV{W0GQR1XS
KYSE-150 NF6yfG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInyZnVKSzVyPUGxMlA{QDdizszN NITTXWhUSU6JRWK=
D-247MG NWTzTXFVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTFzLkGwNFkh|ryP MoTzV2FPT0WU
BxPC-3 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfYOIVRUUN3ME2xNU4{PzR5IN88US=> MXHTRW5ITVJ?
SK-MEL-30 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml36TWM2OD1zMT60NFc6KM7:TR?= MknYV2FPT0WU
CAS-1 NEXQfJNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkfOTWM2OD1zMT60NVA6KM7:TR?= NFy5ZYxUSU6JRWK=
no-10 MkjlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXjJR|UxRTF{LkGyNVch|ryP NEnxfWVUSU6JRWK=
NCI-H2405 NUTFOXc5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG[x[pZKSzVyPUGyMlIxOzZizszN NH7IT|NUSU6JRWK=
KGN MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUPjVmZPUUN3ME2xNk4zQTBzIN88US=> MWHTRW5ITVJ?
BB49-HNC NYXv[IhFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHfzOJFKSzVyPUGyMlM6PTZizszN MWjTRW5ITVJ?
NCI-H1693 NIXwZ3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnHTWM2OD1zMj61PFE3KM7:TR?= MkPoV2FPT0WU
RO82-W-1 NET3ZmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TqcWlEPTB;MUOuNFY1QSEQvF2= Ml;YV2FPT0WU
CAL-85-1 MnfoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPaTWM2OD1zMz60N|k6KM7:TR?= M1vSNHNCVkeHUh?=
H-EMC-SS MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTF|LkmxNFQh|ryP NWKxV2t6W0GQR1XS
SW1783 MnPHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWCzOYFPUUN3ME2xOE4zOzlzIN88US=> M{Tle3NCVkeHUh?=
LB771-HNC MkPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWTFfINmUUN3ME2xOE41OTlizszN MWjTRW5ITVJ?
HCC70 NYD1W21TT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXzbng6UUN3ME2xOE41OjN|IN88US=> MWnTRW5ITVJ?
KNS-81-FD MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVT1SXd3UUN3ME2xOE44ODR3IN88US=> MUnTRW5ITVJ?
KYSE-520 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTF2LkmyO{DPxE1? NFHOd3NUSU6JRWK=
TGBC24TKB NX\nTXJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWP1SGprUUN3ME2xOE46PTN7IN88US=> MWrTRW5ITVJ?
SNB75 M1jxTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\yVWlEPTB;MUWuNFczQSEQvF2= NV;KWYxXW0GQR1XS
SW872 NWLnZ|d6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoPPTWM2OD1zNT6yNlE5KM7:TR?= M4O1dXNCVkeHUh?=
U031 M4fteWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7Y[pBKSzVyPUG1MlgxOjhizszN MUDTRW5ITVJ?
ChaGo-K-1 NFHjXWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULFUG1zUUN3ME2xOU45OzV7IN88US=> MnO5V2FPT0WU
NCI-H441 MkPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHhPFdKSzVyPUG2MlAxOzlizszN NV2xNJV3W0GQR1XS
TE-12 NXHqOmh2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjxd2Z{UUN3ME2xOk4yQDd|IN88US=> M1LhbHNCVkeHUh?=
NCI-H1755 NH3wd4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFizS5VKSzVyPUG2MlU4OyEQvF2= NILYUldUSU6JRWK=
KNS-42 NVTIWFBxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTF4Lk[1PFkh|ryP M1K5PHNCVkeHUh?=
SK-PN-DW NH:zWndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MojjTWM2OD1zNz60NlAyKM7:TR?= M33t[3NCVkeHUh?=
NCI-H226 MoK1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HPU2lEPTB;MUeuPVM1PSEQvF2= MnrCV2FPT0WU
K-562 NVHQNFVqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1HZeWlEPTB;MUiuOlMyPyEQvF2= NU\3U2hMW0GQR1XS
A388 NEXBeXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrrdoprUUN3ME2xPU4xOzB|IN88US=> MVXTRW5ITVJ?
ZR-75-30 M2f5U2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\vTWM2OD1zOT6wPVY5KM7:TR?= MUnTRW5ITVJ?
LU-135 NG\ybGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTF7LkK2N|Yh|ryP M4[0NXNCVkeHUh?=
NCI-H2029 NWfQSpMyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWfJR|UxRTF7Lk[wNlEh|ryP MoXkV2FPT0WU
HT-1197 MnXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHXd4FCUUN3ME2xPU46OjJ5IN88US=> MkXlV2FPT0WU
IGR-1 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonuTWM2OD1{MD6zNVE4KM7:TR?= NVSxcnNXW0GQR1XS
U-87-MG M13jXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn:3TWM2OD1{MD62OFE{KM7:TR?= MUPTRW5ITVJ?
NCI-H1304 NWK4SHRpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTJyLke1NFEh|ryP MoLPV2FPT0WU
RCC10RGB NEPGXI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmTuTWM2OD1{MT6yPFI6KM7:TR?= MXfTRW5ITVJ?
NCI-H520 MmrFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnyflZKSzVyPUKyMlc3OTFizszN MWPTRW5ITVJ?
SK-LU-1 NIT2ZVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXXKbVlKUUN3ME2yN{4xPzF6IN88US=> MmryV2FPT0WU
UACC-893 NUD5eoJmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\xTWM2OD1{Mz6xNFM5KM7:TR?= Mn3OV2FPT0WU
NCI-H2347 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH60bJFKSzVyPUKzMlI3PzRizszN MY\TRW5ITVJ?
U-118-MG M4\rNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTJ|LkeyNVch|ryP MV7TRW5ITVJ?
NCI-H596 M4nTOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUn6NZZ[UUN3ME2yN{46ODV3IN88US=> NFL1NJZUSU6JRWK=
MZ1-PC MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjmbJU{UUN3ME2yOE43PzV7IN88US=> MW\TRW5ITVJ?
SW1417 NYHlUG1YT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV7kV2c6UUN3ME2yOU4zPDl6IN88US=> M3HzPXNCVkeHUh?=
NCI-H82 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEX3bYJKSzVyPUK2MlQzQDhizszN NWnJU2NKW0GQR1XS
COLO-678 NUPlVYk6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7RbIJMUUN3ME2yO{41QTF7IN88US=> MXXTRW5ITVJ?
KURAMOCHI NGrMWlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjQXmhKSzVyPUK4MlEzPiEQvF2= NGnVO5BUSU6JRWK=
NCI-H1650 MlPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnz0TWM2OD1|MD6xPVI1KM7:TR?= M{PSfHNCVkeHUh?=
HCC1954 MoHpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojPTWM2OD1|MT6zOlY3KM7:TR?= MUDTRW5ITVJ?
GAK NFv0WFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorVTWM2OD1|Mj6yOlE3KM7:TR?= M3Hvc3NCVkeHUh?=
YAPC M{TybGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTN|Lke2OFgh|ryP M4SybXNCVkeHUh?=
NCI-H2228 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnnqTWM2OD1|ND6zOlA2KM7:TR?= MYPTRW5ITVJ?
VMRC-RCZ NUDVRXBmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFv6fYpKSzVyPUO2MlMxOjJizszN MmexV2FPT0WU
NMC-G1 NHXMc|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILye5JKSzVyPUO3MlA2OjlizszN MnjKV2FPT0WU
D-336MG MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnkfIxnUUN3ME20NU45PDR{IN88US=> NWXuOFVSW0GQR1XS
UACC-62 M3ThNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEWzfHBKSzVyPUSyMlU2ODVizszN NFrCVIRUSU6JRWK=
C-33-A M4j1eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfUOWNrUUN3ME20OU4{OjV4IN88US=> NW\TZVhYW0GQR1XS
HCC1937 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LiUmlEPTB;NEeuNVk2QCEQvF2= MWLTRW5ITVJ?
HDLM-2 NHW5R|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV3hXG5pUUN3ME20O{4zOjh5IN88US=> MmnyV2FPT0WU
NCI-H727 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XhcmlEPTB;NEeuNlQ2OSEQvF2= NE\BXFdUSU6JRWK=
NY MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTR7LkO5NFgh|ryP Ml[0V2FPT0WU
NCI-H2291 Ml\aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPDTWM2OD12OT64N|AyKM7:TR?= NUP6XoNqW0GQR1XS

... Click to View More Cell Line Experimental Data
体内研究 Decitabine和Vorinostat都具有使肿瘤衰退的功能。然而, Decitabine 和 Vorinostat 联用使肿瘤衰退的效果更强。没有任何处理时,在野生型和Fasgld 小鼠中在肺肿瘤负担方面观察不到明显的区别。然而, Decitabine 和Vorinostat联用处理野生型小鼠，使肿瘤衰退比处理 Fasgld小鼠效果高很多。 Decitabine和Vorinostat使结肠癌细胞对FasL调节的肿瘤衰退敏感。低剂量Decitabine和Vorinostat处理，没有明显的肝脏毒性。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验：[4]
+ 展开

组蛋白去乙酰化酶活性测定:

为了进行 HDAC 实验24 × 104 dpm 乙醇溶液(1 mCi/ml)中提供的sup>3H-醋酸钠盐标记的组蛋白H4等份样, 加到每个试管中，试管中含悬浮在25 µL实验buffer(100 mM Tris-HCl pH 8, 2mM EDTA)中的200 µg全部HeLa细胞裂解液进行免疫沉淀获得的HDAC。加入不同浓度Vorinostat，在30oC下温育90分钟。加入20 µL 终止缓冲液(0.5 N HCl, 0.08 M AcOH)终止反应。加入0.8 mL TBME提取释放放入氚醋酸盐。在8,000 × g 转速下离心5分钟，600 µL 有机相(上相) 转移到包含3 mL闪烁液的闪烁瓶中。
细胞实验：[4]
+ 展开
  • Cell lines: NB4, NCI-H460和HCT-116肿瘤细胞系
  • Concentrations: 0 μM - 0.1 μM
  • Incubation Time: 48小时
  • Method: NB4, NCI-H460和HCT-116肿瘤细胞系生长在200 µL 96孔板中，约10%汇合，生长24小时后，覆盖孔中。使用多种浓度 Vorinostat或溶剂处理肿瘤细胞24小时。处理后，冲洗实验板，移除Vorinostat，温育48小时。使用sulphorodamine B 实验测定Vorinostat处理后存活细胞的分数。
    (Only for Reference)
动物实验：[3]
+ 展开
  • Animal Models: 携带CT26 细胞的Fasgld BALB/cByJ小鼠
  • Formulation: Dissolved in DMSO
  • Dosages: 25 mg/kg
  • Administration: 静脉注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 52 mg/mL (196.74 mM)
Ethanol 3 mg/mL (11.35 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)：
2% DMSO+30% PEG 300+5% Tween 80+ddH2O 		5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 264.3
化学式

C14H20N2O3
CAS号 149647-78-9
稳定性 powder
in solvent
别名 SAHA, MK0683, suberoylanilide hydroxamic acid

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (g) = 浓度 (mol/L) x 体积 (L) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量：

总分子量：g/mol

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03742245 Not yet recruiting Breast Cancer Metastatic|Breast Cancer Jenny C. Chang MD|AstraZeneca|The Methodist Hospital System March 1 2019 Phase 1
NCT03022565 Recruiting Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center December 2018 Early Phase 1
NCT03263533 Recruiting Schizophrenia Virginia Commonwealth University December 1 2018 Early Phase 1
NCT03259503 Not yet recruiting Malignant Neoplasms Stated as Primary Lymphoid Haematopoietic|Lymphoma M.D. Anderson Cancer Center|AstraZeneca November 2018 Phase 1|Phase 2
NCT03713320 Recruiting Cutaneous T-Cell Lymphoma/Mycosis Fungoides miRagen Therapeutics Inc. October 2018 Phase 2
NCT03382834 Active not recruiting HIV Infections National Institute of Allergy and Infectious Diseases (NIAID) April 26 2018 Early Phase 1

技术支持

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操作手册

如果有其他问题，请给我们留言。

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常见问题及建议解决方法

  • 问题 1：

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • 回答：

    We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

Selleck产品目录册

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

相关HDAC产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID