Gemcitabine (LY-188011) HCl

For research use only. Not for use in humans.

目录号:S1149 别名: NSC 613327 中文名称:盐酸吉西他滨

Gemcitabine (LY-188011) HCl Chemical Structure

CAS No. 122111-03-9

Gemcitabine (LY-188011, NSC 613327) HCl是一种DNA synthesis抑制剂,作用于PANC1,MIAPaCa2,BxPC3和Capan2细胞,IC50分别为50 nM, 40 nM, 18 nM和12 nM。

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RMB 1715.34 现货
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客户使用Selleck生产的Gemcitabine (LY-188011) HCl发表文献120篇:

产品安全说明书

DNA/RNA Synthesis抑制剂选择性比较

生物活性

产品描述 Gemcitabine (LY-188011, NSC 613327) HCl是一种DNA synthesis抑制剂,作用于PANC1,MIAPaCa2,BxPC3和Capan2细胞,IC50分别为50 nM, 40 nM, 18 nM和12 nM。
特性 Gemcitabine已经用于治疗胰腺癌,作为最有效的抗癌药物。
靶点
DNA synthesis (Capan2 cells) [1] DNA synthesis (BxPC3 cells) [1] DNA synthesis (MIAPaCa2 cells) [1] DNA synthesis (PANC1 cells) [1]
12 nM 18 nM 40 nM 50 nM
体外研究

Gemcitabine作用于BxPC-3, PANC-1, 和MIA PaCa-2细胞,诱导NF-κB活性,作用于BxPC-3 和PANC-1细胞,降低NF-κB抑制剂 IκBα水平。低剂量Gemcitabine处理BxPC-3细胞48小时提高NF-κB 结合,这种作用存在剂量依赖性。相反, 更高剂量Gemcitabine处理BxPC-3细胞48小时,降低 NF-κB DNA 结合,而更高剂量Gemcitabine处理BxPC-3细胞24小时,提高NF-κB结合。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
CCRF-CEM MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\QbGlEPTB;Mj65JOKyKDFwODDuUS=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
CCRF-CEM/dCK−/− MnjvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3m5[WlEPTB;MkSwMlQhyrFiMkmuNEDPxE1? NYfMd2VLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0NlU5QDVpPkKyOFI2QDh3PD;hQi=>
L1210 wt MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXQTWM2OD1zLkOgxtEhOC5|IH7N M2flb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
L1210 10K MofDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHaTWM2OD1{Mj6yJOKyKDNwNzFOwG0> M3vQR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEK1PFg2Lz5{MkSyOVg5PTxxYU6=
TC-1  M3;Lc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLsTWM2OD1zND63JOKyKDJwODDuUS=> NGXwb4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
TC-1-GR NWrFdmd3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWPXTHJEUUN3ME2zOk44KMLzIEWuNUDPxE1? MkfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2MkW4PFUoRjJ{NEK1PFg2RC:jPh?=
MIA PaCa-2 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\UTWM2OD12OT63JOKyKDF5Lkegcm0> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
PANC-1 M3;oRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NULzNYpsUUN3ME2+JFQxOCEQvF2= NHPB[XA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM NYjNPYNCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGexRnNKSzVyPUKuPUDDuSBzLkigcm0> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR{NUi4OUc,OjJ2MkW4PFU9N2F-
CCRF-CEM-AraC-8C NX6wUGd4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3\sWmlEPTB;OUm4MlghyrFiOT60JI5O NFrtbYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSyOVg5PSd-MkK0NlU5QDV:L3G+
CCRF-CEM  MlrwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HNT|czKGh? NVzBNIgyUUN3ME2yMlAhyrFiMD62JI5O NVjvbHhMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4OVE5PDNpPkKxPFUyQDR|PD;hQi=>
MV-4-11 NXzRboRRT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlfLTWM2OCB;IECuNFAxPSCwTR?= MnTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ES4 M{fKTmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MX7JR|UxKD1iMD6wNFA4KG6P MmnRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ACHN MWnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NYr5dpZVUUN3MDC9JFAvODByOTDuUS=> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
KYSE-510 NWLpNmF[T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= M17rdGlEPTBiPTCwMlAxOSCwTR?= NVvpc3A4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
EW-7 MmLtS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MmnLTWM2OCB;IECuNFAzPiCwTR?= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
BFTC-905 M{m2dGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUHJR|UxKD1iMD6wNFUyKG6P MkDqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
KE-37 M2\lTWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M2TBT2lEPTBiPTCwMlAxPTZibl2= Mn;VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SBC-5 NFuyNZZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NIrTWW9KSzVyIE2gNE4xODV5IH7N NXnLfXhzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
NKM-1 NVzuSppzT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MUnJR|UxKD1iMD6wNFcyKG6P NX\x[Y5YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
RH-1 Ml[5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NUfBcGtxUUN3MDC9JFAvODB5MjDuUS=> MniwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
ALL-PO Mn\1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NH[2eG5KSzVyIE2gNE4xODh|IH7N M{nPOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
QIMR-WIL MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlfuTWM2OCB;IECuNFA5QSCwTR?= M1;ZU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
A375 MUjHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoewTWM2OCB;IECuNFA6QSCwTR?= NFv5[2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
SIG-M5 M1e4PGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWnLfXYyUUN3MDC9JFAvODFyNDDuUS=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
KGN NHHqO4dIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MYnJR|UxKD1iMD6wNVA5KG6P NVf1VlQ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
EW-13 MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3P5bGlEPTBiPTCwMlAyOTJibl2= M{TIPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
NCI-SNU-1 MkDpS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3qyZ2lEPTBiPTCwMlAyPiCwTR?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
PSN1 MkLyS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NXrHS3VkUUN3MDC9JFAvODF4NTDuUS=> NGDpPXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
HUTU-80 Ml[4S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2f5eGlEPTBiPTCwMlAyPjZibl2= NEKzeVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
786-0 M4LDe2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWHaeYpoUUN3MDC9JFAvODJ|IH7N MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
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ES1 M4f0WWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M1;2TGlEPTBiPTCwMlAzPjhibl2= NHO4Uo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw5QDhxJ{7TRW5ITVJ:L3G+
RKO NFTndFNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1\3eWlEPTBiPTCwMlAzPzhibl2= M4mxbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
ESS-1 NEjZd3FIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NX3PW5l6UUN3MDC9JFAvODJ6NjDuUS=> M2TJXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFg5QC9pPmPBUmdGWjxxYU6=
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LB2241-RCC NEPW[FJIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MXrJR|UxKD1iMD6wN|E5KG6P NXnh[ZV3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQDh6Lze+V2FPT0WUPD;hQi=>
CHL-1 MnTaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MYTJR|UxKD1iMD6wN|I1KG6P MlzSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPFg5Nyd-U1HOS2VTRC:jPh?=
SW1783 MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? M3zXRmlEPTBiPTCwMlA{OzZibl2= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
MEL-JUSO M4TJWWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NHTT[WxKSzVyIE2gNE4xOzlzIH7N MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly4PFgwLz6VQV7HSXI9N2F-
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SK-N-AS MoKzR5l1d3SxeHnjbZR6KGG|c3H5 NF:0d|M{KGSjeYO= MYDJR|UxKD1iMT6xJO69VQ>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMUC1OEc,OjF5MUGwOVQ9N2F-
SW1990 NHL5dppEgXSxdH;4bYNqfHliYYPzZZk> M1vzeVczKGi{cx?= MVPJR|UxKD1iMT6yJO69VQ>? MmD3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NkK0O|QoRjF7M{[yOFc1RC:jPh?=
A549 NUjNU4RmS3m2b4TvfIlkcXS7IHHzd4F6 MnjzO|IhcHK| MWTJR|UxKD1iMT60JO69VQ>? M{nYT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
HepG2 MVLGeY5kfGmxbjDhd5NigQ>? M4WzZWtuKD1iMT60JO69VQ>? NX7JT2xjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNFE3PzRpPkG3NVAyPjd2PD;hQi=>
BL21(DE3) M3rnXWZ2dmO2aX;uJIF{e2G7 MV;LcUA:KDFwNEOg{txO Mm\LQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
U87MG MYLDfZRwfG:6aXPpeJkh[XO|YYm= MkGyN{Bl[Xm| M2ewOmlEPTBiPTCxMlQ6KM7:TR?= NYXvT2VjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO|EyODV2PD;hQi=>
BL21(DE3) Mlz4SpVv[3Srb36gZZN{[Xl? MX;LcUA:KDFwNUKg{txO MnvXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
HT-29 MWrDfZRwfG:6aXPpeJkh[XO|YYm= MUCzJIRigXN? M3\0UmlEPTBiPTCxMlU{KM7:TR?= M1nXeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GxNFU1Lz5{MUexNVA2PDxxYU6=
SW1990 NFvNNHhEgXSxdH;4bYNqfHliYYPzZZk> M4nvemlEPTBiPTCxMlYh|ryP MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF7NUS2Okc,OjRzOUW0OlY9N2F-
Capan2 NFzNUW5EgXSxdH;4bYNqfHliYYPzZZk> NIrtUYc4OiCqcoO= MnezTWM2OCB;IEGuO{DPxE1? NIDTSW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
SW480 MmXzRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M1vvUlczKGi{cx?= M1z1dGlEPTBiPTCxMlch|ryP MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh5M{e0NEc,OjB6N{O3OFA9N2F-
PANC1 NYX4doJmS3m2b4TvfIlkcXS7IHHzd4F6 MWW3NkBpenN? M3jyXGlEPTBiPTCxMlch|ryP NYL3[WRHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2OVYzODJpPkK5OlU3OjB{PD;hQi=>
HCT8 MmK3R5l1d3SxeHnjbZR6KGG|c3H5 MYW3NkBpenN? MlTwTWM2OCB;IEGuO|Qh|ryP NGLDOYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO2NlQ4PCd-MUmzOlI1PzR:L3G+
BL21(DE3) NEHHWlFHfW6ldHnvckBie3OjeR?= MmnkT40hRSBzLke1JO69VQ>? MoG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{M{CxN|EoRjJ|MkOwNVMyRC:jPh?=
HT-29 MlH5R5l1d3SxeHnjbZR6KGG|c3H5 NUnoZW5SPDhiaILz NXTx[nZJUUN3MDC9JFEvQTVizszN NFrEbWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m2PFgzPCd-MkO5Olg5OjR:L3G+
MIAPaCa2 NWTuXo9CS3m2b4TvfIlkcXS7IHHzd4F6 NEnyNHQ2KGSjeYO= MlHuSWM2OCB;IEKg{txO M2n4[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OE[3OVkxLz5{NEi2O|U6ODxxYU6=
BL21(DE3) NX3GenpVTnWwY4Tpc44h[XO|YYm= M3WwOVEhdWmw MnO4T40hRSB{LkG1JO69VQ>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ|MEGzNUc,OjN{M{CxN|E9N2F-
SW1990 MmjqR5l1d3SxeHnjbZR6KGG|c3H5 MoXOTWM2OCB;IEKuNkDPxE1? M4DaTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MUC1O|IzLz5{NUGwOVczOjxxYU6=
SW1990 NYPFS|JrS3m2b4TvfIlkcXS7IHHzd4F6 M4jlW|Q5KGi{cx?= M1rjXWlEPTBiPTCyMlMh|ryP MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzl4Nkm1NEc,Ojd7Nk[5OVA9N2F-
NCI-H146 NIq5PGZEgXSxdH;4bYNqfHliYYPzZZk> NGrtT24{KGSjeYO= NWixZVVzUUN3MDC9JFIvPzhizszN NHLSdJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNVA2PCd-MkG3NVExPTR:L3G+
BxPC3 MnjaR5l1d3SxeHnjbZR6KGG|c3H5 MnXZO|IhcHK| NHvhc2ZKSzVyIE2gNk46KM7:TR?= M4Tic|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
p53 deficient COLO205 MVLBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MUW3NkBpenN? NYLCOII3UUN3MDC9JFMh|ryP M4PpVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEezO|QxLz5{MEi3N|c1ODxxYU6=
MiaPaCa MnTDR5l1d3SxeHnjbZR6KGG|c3H5 NWHl[Wh5PiCmYYnz NEPtR5lKSzVyIE2gN{DPxE1? NHTufXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O2NFExPCd-MkOzOlAyODR:L3G+
COLO205 MXzDfZRwfG:6aXPpeJkh[XO|YYm= NH[0dXo1QCCqcoO= M4fGPGlEPTBiPTCzMlI1KM7:TR?= NX\5eXc5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5Olg5OjRpPkKzPVY5QDJ2PD;hQi=>
MCF7 MkjqR5l1d3SxeHnjbZR6KGG|c3H5 MWm3NkBpenN? NHjvWGJKSzVyIE2gN{4zQCEQvF2= M3zrcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{[yOFc1Lz5zOUO2NlQ4PDxxYU6=
MIAPaCa2 NIK2eFVEgXSxdH;4bYNqfHliYYPzZZk> MWG3NkBpenN? NEHtO4ZKSzVyIE2gN{4{KM7:TR?= NIPhTJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUOyPFY2Pid-MkmzNlg3PTZ:L3G+
HeLa M2jkXWN6fG:2b4jpZ4l1gSCjc4PhfS=> M2rnRVI1KGi{cx?= NXzxWJNiUUN3MDC9JFMvOyEQvF2= NILwdG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUewN|I6Pid-MkW3NFMzQTZ:L3G+
FTC-133 MWjDfZRwfG:6aXPpeJkh[XO|YYm= M{TDflI1KGi{cx?= MlPOTWM2OCB;IEOuN|Yh|ryP MonUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2M{[5PVQoRjJ2NEO2PVk1RC:jPh?=
BxPC3 NETRNVlIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NW[3cXNKPDhiaILz M2LlT2dKPTBiPTCzMlY1KM7:TR?= M4DzW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUGyPVA5Lz5{MkWxNlkxQDxxYU6=
COLO320DM M{LJWmN6fG:2b4jpZ4l1gSCjc4PhfS=> MmWyOFghcHK| Mkf3TWM2OCB;IEOuPVIh|ryP MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl4OEiyOEc,OjN7Nki4NlQ9N2F-
HeLa MnjmR5l1d3SxeHnjbZR6KGG|c3H5 M4nHS|Mh\GG7cx?= Mom2TWM2OCB;IESuNVIh|ryP NXW3RWp3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO|EyODV2PD;hQi=>
8305C M3;hR2N6fG:2b4jpZ4l1gSCjc4PhfS=> MUWyOEBpenN? MlHiTWM2OCB;IESuOVMh|ryP NE\6eFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NESzOlk6PCd-MkS0N|Y6QTR:L3G+
PANC1 NF33WWtEgXSxdH;4bYNqfHliYYPzZZk> Mk[0O|IhcHK| MmfPTWM2OCB;IEWuOkDPxE1? MkTzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|NkK0O|QoRjF7M{[yOFc1RC:jPh?=
PANC1 MVrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NIHXNnk4OiCqcoO= NGm5UFJIUTVyIE2gOU45KM7:TR?= NEC0flQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OES5OVA5OSd-Mki0PVUxQDF:L3G+
SK-MEL-2 NHfjU2tEgXSxdH;4bYNqfHliYYPzZZk> NES2V2U{KGSjeYO= NWTaU|NwUUN3MDC9JFcvOTFizszN MlPTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUGwOVQoRjJzN{GxNFU1RC:jPh?=
CEM NUfCUIw2S3m2b4TvfIlkcXS7IHHzd4F6 Mmj3N{Bl[Xm| MUDJR|UxKD1iNz62JO69VQ>? NF;UUm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO0NVM2Pid-MkSzOFE{PTZ:L3G+
SW1573 NX64dGZoS3m2b4TvfIlkcXS7IHHzd4F6 NEPVfpE4OiCqcoO= Mmm0TWM2OCB;IEiuN{DPxE1? NE[wfnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{SxPVYxPCd-MUe0NVk3ODR:L3G+
BxPC3 NWLRPYY4T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYC0PEBpenN? NIiyXmtNSzVyIE2gPE44OSEQvF2= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjVzMkmwPEc,OjJ3MUK5NFg9N2F-
MCF7 M4rLUGN6fG:2b4jpZ4l1gSCjc4PhfS=> MlTHO|IhcHK| NY[4N5VvUUN3MDC9JFgvQSEQvF2= MmLpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyN{W1PVIoRjJ6MEe1OVkzRC:jPh?=
BJ NX:1eJh2S3m2b4TvfIlkcXS7IHHzd4F6 NFrYXGk{KGSjeYO= NE\TcmpKSzVyIE2gPU45QCEQvF2= NYnz[FB5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVExPTRpPkKxO|EyODV2PD;hQi=>
HeLa MVnDfZRwfG:6aXPpeJkh[XO|YYm= M2DjVFczKGi{cx?= MoL6TWM2OCB;IEGwJO69VQ>? NWLz[5psRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2N|E{PTlpPkK0OlMyOzV7PD;hQi=>
B16-F10-Luc-G5 NVi4Um8zSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MUeyOEBpenN? MmTGTWM2OCB;IEGxJO69VQ>? NGOxOFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{O0PVM{Oid-MkezOFk{OzJ:L3G+
HCT15 M{Gz[WN6fG:2b4jpZ4l1gSCjc4PhfS=> NYHKeZNSUUN3MDC9JFEyNjhizszN MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDF6Nk[wOEc,OThzOE[2NFQ9N2F-
A549 MWDDfZRwfG:6aXPpeJkh[XO|YYm= M1\neVczKGi{cx?= MULJR|UxKD1iMUOuNUDPxE1? MkfvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2MUm2NFQoRjF5NEG5OlA1RC:jPh?=
HCT116 MYfDfZRwfG:6aXPpeJkh[XO|YYm= M{DtSmlEPTBiPTCxOE4{KM7:TR?= NX\uVng3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUixPFY3ODRpPkG4NVg3PjB2PD;hQi=>
MiaPaCa NWSwb2JLS3m2b4TvfIlkcXS7IHHzd4F6 M2nMOVczKGi{cx?= NVHJS5JtUUN3MDC9JFE4NjFizszN MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR6OU[yOkc,OjN2OEm2NlY9N2F-
AG6000 NXnqWo8xT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NXrjPWtkUUN3MDC9JFIxKM7:TR?= M2O1TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkCyOFY1Lz5zN{[wNlQ3PDxxYU6=
AG6000 NXjwN5BZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NWrDc|VzUUN3MDC9JFIxKM7:TR?= NXX2OI1PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NFI1PjRpPkG3OlAzPDZ2PD;hQi=>
BxPC3 MYfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M3TETlczKGi{cx?= NGP5b2pKSzVyIE2gNlAvOiEQvF2= MlvRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3N{[2N|MoRjJ6NUe2OlM{RC:jPh?=
CV1 Ml;0R5l1d3SxeHnjbZR6KGG|c3H5 NULVbJlIPzJiaILz M4ewcWlEPTBiPTCyNU46KM7:TR?= MmrYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OEm2NlYoRjJ|NEi5OlI3RC:jPh?=
AsPC1 NEXj[FVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MVi3NkBpenN? NFLJT2dKSzVyIE2gNlYvQCEQvF2= NEjEd2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEW3OlY{Oyd-Mki1O|Y3OzN:L3G+
PANC1 NWP1[npMSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MVO3NkBpenN? NYPHU5ZiUUN3MDC9JFMxNjRizszN NWTOb3JzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1O|Y3OzNpPkK4OVc3PjN|PD;hQi=>
MIAPaCa2 NVf1e4FrT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NVTITnNHPDhiaILz MXfHTVUxKD1iM{WuPFMh|ryP M2\US|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NUGyPVA5Lz5{MkWxNlkxQDxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-JNK / JNK / p-ATF2 / p-c-Jun; 

PubMed: 16307741     


Time-dependent activation of the JNK signaling pathway by gemcitabine treatment. H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods. Cell lysates were then subjected to western blotting using total JNK and phospho-specific antibodies to JNK, c-Jun, and ATF2. Maximal phosphorylation of those molecules is observed in H1299 cells treated with gemcitabine for 48 h.

caspase-3 / caspase-8 / caspase-9; 

PubMed: 16307741     


H1299 and H1299/GR cells were treated with 100 nM gemcitabine for the indicated time periods, and proteins in whole-cell lysates were analyzed by immunoblotting with anti-caspase-3, caspase-8, and caspase-9 antibodies. The arrowheads represent cleavage proteins. β-actin was used as a loading control.

pS-ERα / ERα / pERK / ERK; 

PubMed: 27384485     


MCF-7 and MDA-MB-231 cells were treated by gemcitabine with different concentrations (MCF-7: 0, 2, 4, 8 μg/ml; MDA-MB-231: 0, 0.5, 1, 2 μg/ml), and the phosphorylation of ERα (ser167), ERK (Thr202/Tyr204) and expression of LC3-I and LC3-II were detected.

LC3-I / LC3-II; 

PubMed: 27384485     


A, E. The levels of LC3-I and LC3-II detected by Western blotting were quantified by densitometry using Image J software. The ratio of LC3-II to LC3-I was evaluated after treated with gemcitabine (MCF-7: 4 μg/ml; MDA-MB-231: 1 μg/ml) for 0 h (control), 12, 24 and 48 h. B, F. The ratio of LC3-II to actin was assayed after treated with gemcitabine, chloroquine (CQ) (MCF-7: 2.5 μM; MDA-MB-231: 5 μM), and gemcitabine plus CQ (added before gemcitabine treatment for 1 h) for indicated time (MCF-7: 48 h; MDA-MB-231: 24 h).

p70S6K1 / p-S6 / HIF-1α; 

PubMed: 27765914     


Cells were treated with different doses of gemcitabine (0, 1.5 and 2.5 μM), the expression levels of p70S6K1, p-S6 and HIF-1α were determined by Western blotting. GAPDH is used as an internal control. 

16307741 27384485 27765914
Immunofluorescence
BMI1; 

PubMed: 27177084     


Gemcitabine enhanced nuclear accumulation of Bmi1 by immunofluorescence staining. 

27177084
Growth inhibition assay
Cell viability ; 

PubMed: 27765914     


A and B. Pancreatic adenocarcinoma cells Bxpc-3 (A) and Panc-1 (B) were treated with gemcitabine (GEM) for 48 h at different doses. The cell viability was analyzed by CCK-8 assay, and normalized to cells without gemcitabine treatment.

27765914
体内研究 与PBS处理的小鼠相比,Gemcitabine处理的小鼠瘤内NF-κB 活性显著提高(提高1.3- 到1.8-倍),说明 Gemcitabine也诱导NF-κB 活性。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:[2]
- 合并
  • Cell lines: BxPC-3, MIA PaCa-2, 和 PANC-1 细胞
  • Concentrations: 0.2 μM
  • Incubation Time: 24 小时或48小时
  • Method: BxPC-3, MIA PaCa-2, 和PANC-1 细胞接种在96孔板中。24小时后,使用 DMAPT 和/或 Gemcitabine处理24小时或48小时。使用细胞死亡检测 ELISA测定细胞质组蛋白相关 DNA片段的量,而量化细胞凋亡。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: 携带MIA PaCa-2细胞的无胸腺裸鼠
  • Dosages: 50 mg/kg或100 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 Water 19 mg/mL (63.4 mM)
DMSO Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
Saline
19mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 299.66
化学式

C9H11F2N3O4.HCI

CAS号 122111-03-9
储存条件 粉状
溶于溶剂
别名 NSC 613327

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置