KU-0063794

For research use only. Not for use in humans.

目录号:S1226

KU-0063794 Chemical Structure

CAS No. 938440-64-3

KU-0063794是一种有效的,高度特异性的,作用于mTORC1mTORC2的双重mTOR抑制剂,在无细胞试验中IC50约为~10 nM;对PI3Ks没有作用。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1464.74 现货
RMB 727.74 现货
RMB 1405.09 现货
RMB 3857.09 现货
RMB 5488.69 现货
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客户使用Selleck生产的KU-0063794发表文献65篇:

产品安全说明书

mTOR抑制剂选择性比较

生物活性

产品描述 KU-0063794是一种有效的,高度特异性的,作用于mTORC1mTORC2的双重mTOR抑制剂,在无细胞试验中IC50约为~10 nM;对PI3Ks没有作用。
靶点
mTORC1 [1]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
~10 nM ~10 nM
体外研究

与mTOR抑制剂PP242相比, KU-0063794高特异性作用于mTOR,而对PI3Ks或其他76种激酶则无作用活性。30 nM KU-0063794作用于HEK-293细胞,通过抑制疏水基团(Thr389)磷酸化,及随后的T-环残基(Thr229) 磷酸化,而快速切除S6K1活性。300 nM KU-0063794作用于IGF1刺激的血清饥饿处理的 HEK-293细胞,抑制~90%S6K1活性。KU-0063794 为 100-300 nM时,也完全抑制氨基酸诱导的S6K1和 S6蛋白磷酸化。与S6K1类似, KU-0063794抑制mTORC1在 Ser2448位点磷酸化,也抑制mTORC2 在 Ser22481 位点磷酸化,这种作用存在剂量和时间依赖性。在血清存在时,或者IGF1刺激的情况下, KU-0063794抑制Akt 活性,或者Akt在 Ser473和 Thr308(意外的)位点磷酸化,也抑制Akt底物 PRAS40 在 Thr246位点,GSK3α/GSK3β在 Ser21/Ser9位点及Foxo-1/3a 在 Thr24/Thr32位点磷酸化,以上作用存在剂量依赖性。KU-0063794而不是 Rapamycin 抑制SGK1活性和 在Ser422位点磷酸化,也抑制其生理底物NDGR1,与抑制S6K1 和Akt磷酸化程度相似,这种作用存在剂量依赖性,然而KU-0063794 不抑制佛波酯诱导的ERK 或RSK磷酸化和RSK激活。与Rapamycin相比, KU-0063794更有效诱导4E-BP1在 Thr37, Thr46和 Ser65位点完全去磷酸化。KU-0063794抑制野生型和mLST8-缺陷型MEFs细胞生长,也诱导细胞周期停在G1期,比Rapamycin效果更显著。[1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HepG2  NHzKSJRE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NUHzW5Y3OC5z4pETOVDDqM7:TR?= NVTQZphsPzJiaB?= NFPqOYtl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlJIRmeGWwZHXueEBu[W6wZYK= M1T2VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Mke4PFE6Lz5{NkK3PFgyQTxxYU6=
HepG2  NV;DW|N3S2:ub375JGZwem2jdHnvckBCe3OjeR?= NUi0WW9DOeLCk{WwxsDPxE1? MWOxNEBl M4PiS4Rm[3KnYYPld{B1cGViboXtZoVzKG:oII\pZYJt\SCKZYDHNkBkd2yxbnnld:Khe2mpbnnmbYNidnSueR?= MlPyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ{N{i4NVkoRjJ4Mke4PFE6RC:jPh?=
HepG2  M{\VdmFxd3C2b4Ppd{BCe3OjeR?= NUT2Tmx1OC5z4pETOVDDqM7:TR?= NYfuSJZwPDhiaB?= NH\OOYRqdmS3Y3XzJIFxd3C2b4Ppd{BqdiCjIHTvd4Uh\GWyZX7k[Y51KG2jbn7ldi=> NXvuOoNFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[yO|g5OTlpPkK2Nlc5QDF7PD;hQi=>
HepG2  NGrPZndHfW6ldHnvckBCe3OjeR?= MoPJOU8yOCEQvF2= NHTDXm0zPCCq MUnkdoFu[XSrY3HscJkhcW6qaXLpeJMheGixc4Doc5J6dGG2aX;uJI9nKEGNVDDheEBU\XJvNEez M{C2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Mke4PFE6Lz5{NkK3PFgyQTxxYU6=
HepG2  NYG2RVVHTnWwY4Tpc44hSXO|YYm= M1TaflUwOTBizszN NY\zbVBPOjRiaB?= M2TL[4Rwf26{ZXf1cIF1\XNidHjlJIxmfmWuczDITWYy|rFiYX7kJIN6[2yrbjDENeKh M{jGclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Mke4PFE6Lz5{NkK3PFgyQTxxYU6=
HepG2  MkL1SpVv[3Srb36gRZN{[Xl? M3zjSVAvOeLCk{WwxsDPxE1? MWmyOEBp MYDpcoR2[2W|IIC2NkBld3ewcnXneYxifGmxbjygRoVkdGmwLUGg[ZhxemW|c3nvckBidmRiTFOzRk1KKHSxIFzDN2IuUUliY3;ueoVze2mxbjDpckBiKGSxc3Wg[IVx\W6mZX70JI1idm6nch?= M3ryPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4Mke4PFE6Lz5{NkK3PFgyQTxxYU6=
HepG3  NVPxWFJsTnWwY4Tpc44hSXO|YYm= Mli3NE4y6oDVNUFCpO69VQ>? M4XISFQ5KGh? M4j6WIlv\HWlZYOgZ4VtdCCjdYTvdIhi\3liaX6gZUBld3OnIHTldIVv\GWwdDDtZY5v\XJ? NIPpNYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkK3PFgyQSd-Mk[yO|g5OTl:L3G+
AGS NUnxbpl6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzwVGlKSzVyPUG1MlAhyrFiMj65NUDPxE1? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV7N{S3PEc,OjR3OUe0O|g9N2F-
HGC27 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;yN5NKSzVyPUG1MlAhyrFiND64NkDPxE1? NFzvVGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW5O|Q4QCd-MkS1PVc1Pzh:L3G+
MKN45 M3PRNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoTWTWM2OD1yLkiyJOKyKDBwMEGg{txO MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV7N{S3PEc,OjR3OUe0O|g9N2F-
NUGC4 M1;teWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXqZWljUUN3ME2yMlk{KMLzIECuN|Eh|ryP NILUbGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW5O|Q4QCd-MkS1PVc1Pzh:L3G+
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PC9GR MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkL6O|IhcA>? MmXrTWM2OD14LkKxxtEyNjNyIH7N NYj0bZI2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O|Q5QDBpPkKzPFc1QDhyPD;hQi=>
H1650 M4rXb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY[3NkBp NUHJSHBvUUN3ME23MlYyyrFyLk[yJI5O MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh5NEi4NEc,OjN6N{S4PFA9N2F-
H1975 NEL0bWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUm3NkBp NYHqV4N6UUN3ME2xNU4yPcLzMD65N{BvVQ>? MkG0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{S4PFAoRjJ|OEe0PFgxRC:jPh?=
PC9 NYnjXY5vTnWwY4Tpc44hSXO|YYm= M3;W[FExNjF3IH7N MnLzO|IhcA>? NHq1fVBqdmirYnn0d{BuXE:UIIDoc5NxcG:{eXzheIlwdiC|dHH0eZPDqA>? NGrrbXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3OFg5OCd-MkO4O|Q5QDB:L3G+
PC9GR MXjGeY5kfGmxbjDBd5NigQ>? NXvjTpBYPi5{MTDuUS=> Mo\rO|IhcA>? NGTD[pNqdmirYnn0d{BuXE:UIIDoc5NxcG:{eXzheIlwdiC|dHH0eZPDqA>? NHfETGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i3OFg5OCd-MkO4O|Q5QDB:L3G+
H1650 M3WzRmZ2dmO2aX;uJGF{e2G7 NHqxc5A4NjZzIH7N NGr1U3M4OiCq Ml;UbY5pcWKrdIOgcXRQWiCyaH;zdIhwenmuYYTpc44he3SjdIXzxsA> M4HBVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEe0PFgxLz5{M{i3OFg5ODxxYU6=
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PC9 NUntSVRPTnWwY4Tpc44hSXO|YYm= M{jjclExNjF3IH7N MoXLO|IhcA>? NES0dG5qdmirYnn0d{BxcG:|cHjvdplt[XSrb36gc4YheDdyU{\L M4[2[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEe0PFgxLz5{M{i3OFg5ODxxYU6=
PC9GR MofmSpVv[3Srb36gRZN{[Xl? NFTl[4g3NjJzIH7N MoLwO|IhcA>? NYjnSGNxcW6qaXLpeJMheGixc4Doc5J6dGG2aX;uJI9nKHB5MGO2Ty=> NVTXXoVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O|Q5QDBpPkKzPFc1QDhyPD;hQi=>
H1650 MYLGeY5kfGmxbjDBd5NigQ>? M1jiNFcvPjFibl2= NWPUUYtMPzJiaB?= NVK1eGNxcW6qaXLpeJMheGixc4Doc5J6dGG2aX;uJI9nKHB5MGO2Ty=> NV:ySZJmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4O|Q5QDBpPkKzPFc1QDhyPD;hQi=>
H1975 MX3GeY5kfGmxbjDBd5NigQ>? M3fUT|EyNjF3IH7N M3Gxe|czKGh? MnXjbY5pcWKrdIOgdIhwe3Cqb4L5cIF1cW:wIH;mJJA4OFN4Sx?= M17BVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEe0PFgxLz5{M{i3OFg5ODxxYU6=
LNCaP NHXlUIJE\WyuIG\pZYJqdGm2eTDBd5NigQ>? MXewMVExKM7:TR?= MlfhNlQhcMLi NVfmZ283\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIlvKGFiZH;z[UBl\XCnbnTlcpQhdWGwbnXy M2XFNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OESwOlA2Lz5{M{i0NFYxPTxxYU6=
PC-3 NWiwOGFbS2WubDDWbYFjcWyrdImgRZN{[Xl? M3;vd|AuOTBizszN M3LKdlI1KGkEoB?= NF\HOG5l\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgbY4h[SCmb4PlJIRmeGWwZHXueEBu[W6wZYK= MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh2ME[wOUc,OjN6NEC2NFU9N2F-
MDA-MB-468  MU\D[YxtKF[rYXLpcIl1gSCDc4PhfS=> NGfkSlMxNTFyIN88US=> MVuyOEBpyqB? MorC[IVkemWjc3XzJINmdGxidnnhZoltcXS7IHnuJIEh\G:|ZTDk[ZBmdmSnboSgcYFvdmW{ MkmxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NEC2NFUoRjJ|OESwOlA2RC:jPh?=
LNCaP NHG2SZVHfW6ldHnvckBCe3OjeR?= NFv0dGszODEkgKO4NFAhdk1? NYjQTWtDOjRiaNMg MXLk[YNz\WG|ZYOgeIhmKHCqb4PwbI9zgWyjdHnvckBt\X[nbDDv[kBxPzCVNlugbY4h[SCmb4PlJIRmeGWwZHXueEBu[W6wZYK= NETUTFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0NFYxPSd-MkO4OFA3ODV:L3G+
PC-3 MkT1SpVv[3Srb36gRZN{[Xl? NELrXZAzODEkgKO4NFAhdk1? MYmyOEBpyqB? MnGw[IVkemWjc3XzJJRp\SCyaH;zdIhwenmuYYTpc44hdGW4ZXygc4YheDdyU{\LJIlvKGFiZH;z[UBl\XCnbnTlcpQhdWGwbnXy NEnpPZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i0NFYxPSd-MkO4OFA3ODV:L3G+
MDA-MB-468  M3vSTmZ2dmO2aX;uJGF{e2G7 MYiyNFDjiJN6MECgcm0> MYmyOEBpyqB? NILTRVJl\WO{ZXHz[ZMhfGinIIDoc5NxcG:{eXzheIlwdiCuZY\lcEBw\iCyN{DTOmshcW5iYTDkc5NmKGSncHXu[IVvfCCvYX7u[ZI> MlHWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6NEC2NFUoRjJ|OESwOlA2RC:jPh?=
Caki-1  NX6xcGxVTnWwY4Tpc44hSXO|YYm= NVPkfnpsOTByLUKwNFAhdk1? NFnMU|AyOC1zOECgcYlv NHHjRoFFVVOR NGj5OJZqdmirYnn0d{Bjd3SqIH3UU3JEOSCjbnSgcXRQWkN{IHHzJIlv\GmlYYTl[EBjgSC2aHWg[IVkemWjc3WgbY4heGixc4Doc5J6dGG2aX;uJI9nKGSxd37zeJJm[W1iZX\m[YN1d3K| NYDIUpBjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOzOFk6QDlpPkKzN|Q6QTh7PD;hQi=>
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Caki-1  MnHoR4VtdCCYaXHibYxqfHliQYPzZZk> NFrZSnY{ODBvNECwNEBvVQ>? NXjuVYRNOjRvOU[gbC=> NYfjV3dDTE2VTx?= MWnzeZBxemW|c3XzJJRp\SClZXzsJJZq[WKrbHn0fUBqdiCkb4ToJJRqdWViYX7kJIRwe2ViZHXw[Y5l\W62IH3hco5meg>? M4Hk[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|M{S5PVg6Lz5{M{O0PVk5QTxxYU6=
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Caki-1  NHPHUGJHfW6ldHnvckBCe3OjeR?= M{PhOlIhyrWP NFfVc5M4OiCq M3;qUWROW09? M2\LfIlv\HWlZYOgS|Eh[2WubDDjfYNt\SCjcoLld5Qh[W6mIHH1eI9xcGGpeR?= NH;4SXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{O0PVk5QSd-MkOzOFk6QDl:L3G+
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-S6K / S6K / p-4E-BP1 / E7 / E6 / p53 ; 

PubMed: 28115701     


Normoxic HPV-positive cancer cells were treated for 24 h with 0.5, 1.0, or 5 μM KU-0063794 (KU) or 50 nM rapamycin. Shown are immunoblots of P-S6K, S6K, P-4E-BP1, 4E-BP1, HPV16/18 E6/E7, and p53 protein levels. DMSO served as a solvent control; β-actin, as a loading control.

p-mTOR; 

PubMed: 24262658     


Inhibition of mTOR pathway by mTOR kinase inhibitors. MCC-2 cells were treated with WYE354 (3 μM), PP242 (2.5 μM), and Ku-0063794 (5 μM) for 24h, respectively. Lysates were prepared and subjected to immunoblotting analysis with indicated antibodies. 

28115701 24262658
体内研究 Ku0063794在肾脏上皮肾细胞癌的临床前模型中,抑制肿瘤生长和mTOR信号。然而,在动物研究中,Ku0063794没有temsirolimus有效,可能的原因是temsirolimus对肿瘤的微环境有重要的作用,能在异种移植瘤中减少血管生成,而Ku0063794无此效果。经Temsirolimus处理的肿瘤组织比Ku0063794处理的肿瘤组织更少地表达VEGF和PDGF[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

mTOR 复合物激酶检测实验:

HEK-293细胞新鲜溶解在Hepes裂解液中。溶解产物(1-4 mg) 通过与5-20 μL G蛋白的琼脂糖凝胶结合免疫前抗体IgG温育而预先清除。裂解抽提物与5-20 μL G蛋白的琼脂糖凝胶结合的 5-20 μg抗Rictor或抗Raptor抗体的 免疫前抗体IgG温育。所有抗体共价结合到蛋白G蛋白琼脂糖凝胶上。在振动转载台上4oC下进行 免疫沉淀1小时。使用Hepes 裂解液冲洗免疫沉淀物四次,随后使用Hepes激酶buffer冲洗两次。使用 Raptor免疫沉淀物磷酸化 S6K1, 两次冲洗的初始步骤中,buffer 含0.5 M NaCl,确保最佳激酶活性。血清饥饿的 HEK-293细胞中分离的GST-Akt1与PI-103(1 μM 进行 1 小时)温育。从血清饥饿的HEK-293 细胞中纯化的GST-S6K1与Rapamycin (0.1 μM进行1小时)温育。加入0.1 mM ATP和 10 mM MgCl2 ,在不同浓度KU-0063794和GST-Akt1 (0.5 μg)或GST-S6K1 (0.5 μg)存在时,开始mTOR反应。反应在 30oC下在振动转载台上进行30分钟,然后加入SDS样本缓冲液终止反应。反应混合物在0.22-μm-孔径大小 Spin-X过滤器上过滤,样本进行电泳处理,然后使用指定抗体进行免疫印迹分析。
细胞实验:

[1]

- 合并
  • Cell lines: 野生型和mLST8缺陷型MEFs
  • Concentrations: 溶于DMSO,终浓度为~3 μM
  • Incubation Time: 24, 48, 和72 小时
  • Method:

    使用KU-0063794 处理细胞 24, 48, 和72 小时,每24小时更换一次培养基,加入新鲜溶解的KU-0063794。为了测量细胞生长,使用PBS冲洗细胞一次,然后与4% (v/v) 多聚甲醛在 PBS混合15分钟。用水冲洗一次后,使用溶于 10% 乙醇的0.1% 结晶紫对细胞染色20分钟,然后用水冲洗三次。 使用0.5 mL 10% (v/v) 乙酸从细胞中抽提结晶紫20分钟。然后洗脱液在水中按1:10稀释,然后在 590 nm 处测量吸光值。为了测量细胞周期分布,通过胰蛋白酶化作用收集细胞,然后在PBS中冲洗一次,再悬浮于冰冻70% (v/v)乙醇中。细胞在 PBS和 1% (w/v) BSA中冲洗两次,再在 PBS 和含 50 g/mL 碘化丙啶和 50 g/mL RNase A的0.1% (v/v) Triton X-100中染色 20分钟。使用FACSCalibur流式细胞仪和CellQuest软件检测细胞中DNA含量。在线性标度上测量红色荧光(585 nm),进行脉冲宽度分析用于排除双峰值。使用FlowJo软件测定细胞周期分布。


    (Only for Reference)
动物实验:

[2]

- 合并
  • Animal Models: Nu/Nu裸鼠
  • Dosages: 8 mg/kg
  • Administration: i.p.
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 16 mg/mL (34.36 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
30% PEG400+0.5% Tween80+5% propylene glycol
30 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 465.54
化学式

C25H31N5O4

CAS号 938440-64-3
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID