AZD8055

For research use only. Not for use in humans.

目录号:S1555

AZD8055 Chemical Structure

CAS No. 1009298-09-2

AZD8055是一种新型的,ATP竞争性mTOR抑制剂,在MDA-MB-468 细胞中IC50为0.8 nM,与作用于PI3K亚型和ATM/DNA-PK相比,具有优异的选择性(约1000倍)。AZD8055可诱导半胱天冬酶依赖的凋亡和自噬。Phase 1。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1265.49 现货
RMB 988.31 现货
RMB 3048.72 现货
有超大折扣
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客户使用Selleck生产的AZD8055发表文献224篇:

产品安全说明书

mTOR抑制剂选择性比较

生物活性

产品描述 AZD8055是一种新型的,ATP竞争性mTOR抑制剂,在MDA-MB-468 细胞中IC50为0.8 nM,与作用于PI3K亚型和ATM/DNA-PK相比,具有优异的选择性(约1000倍)。AZD8055可诱导半胱天冬酶依赖的凋亡和自噬。Phase 1。
特性 AZD8055是第一种抑制两种类型mTOR蛋白的药物,被认为可能比之前mTOR抑制剂更有效。
靶点
mTOR (truncated) [1]
(MDA-MB-468 cells)
mTOR (full length) [1]
(MDA-MB-468 cells)
0.13 nM 0.8 nM
体外研究

AZD8055作用于所有 PI3K亚型 (α, β, γ, δ) 和其他近PI3K激酶家族(ATM和DNA-PK)显示出低活性。AZD8055抑制mTORC1(p70S6K 和4E-BP1) ,mTORC2 (AKT) 和下游蛋白的磷酸化。AZD8055完全抑制4E-BP1抗Rapamycin 的T37/46 磷酸化位点, 导致明显抑制cap-dependent 转译作用。AZD8055有效抑制U87MG, A549和H838 细胞增殖, IC50分别为53, 50,和20 nM。AZD8055作用于H838和A549细胞,也诱导自体吞噬和增加LC3-II 水平。[1] AZD8055降低白血病细胞增殖和细胞周期进展, 降低白血病祖细胞的无性系生长,且在白血病细胞中诱导caspase依赖的细胞凋亡,但是作用于正常未成熟的CD34+ 细胞时不会产生这种诱导作用。[2] AZD8055 抑制儿科临床前期测试计划(PPTP)细胞系,IC50 为24.7 nM,且在EFS 分布时诱导产生明显区别。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
LU-139 M4fEVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\RRnVKUUN3ME2wMlAzOzNzIN88US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NCI-H720 M3fm[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYPJR|UxRTBwMEK5N|gh|ryP NEjkVnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
KARPAS-45 NEO0Z4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTBwMEOzO|Eh|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
EW-3 NXjNepNwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTBwMESwOVYh|ryP NFPrcoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
SBC-1 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mm\4TWM2OD1yLkC0OVgyKM7:TR?= M3;IbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
NCI-H209 M1\mSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXvTWM2OD1yLkC1OFU{KM7:TR?= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
EoL-1-cell NWjzbZRyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXRTWM2OD1yLkC2OVY6KM7:TR?= M4DIPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
H9 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTYTWM2OD1yLkC2OlkzKM7:TR?= M1PublxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
AN3-CA M331VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTBwMEe5OVEh|ryP NXLjWIdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
HH NF20OGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkHITWM2OD1yLkC4NFA6KM7:TR?= M3jCW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
KU812 NVzaSlE3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rNTGlEPTB;MD6wPFA1OyEQvF2= MkDiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
DU-4475 M3HGR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\3TWM2OD1yLkC4NFQ4KM7:TR?= NWPORlhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
CTV-1 NUK3XWs1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTBwMEi2OFEh|ryP NY[zXpBORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
BV-173 M4rNcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3u4UGlEPTB;MD6wPVA3OSEQvF2= NVyy[FFyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
ES5 M2qweGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlL2TWM2OD1yLkC5N|Uh|ryP MlntQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
G-402 MmD2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\pUWlEPTB;MD6wPVk5KM7:TR?= NGfPe|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
MV-4-11 NVjJdlNCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvzTWM2OD1yLkGwN|Q4KM7:TR?= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NB69 NXTLcZpNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTBwMUO1JO69VQ>? Ml7lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
EGI-1 MonrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGL5dFVKSzVyPUCuNVM2OzFizszN MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
EW-18 Mln0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWfJR|UxRTBwMUSwO|Mh|ryP NUT0eYZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
ME-180 MlXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2O0U2lEPTB;MD6xOFY5OSEQvF2= NYHLR3d7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
D-283MED MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXrBRWJtUUN3ME2wMlE1PzJ|IN88US=> NVKwR|Y6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
NB14 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTBwMU[0OFch|ryP MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
Ramos-2G6-4C10 NYizUYRJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnvkTWM2OD1yLkG2PVg{KM7:TR?= NHvMSpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
NBsusSR NGThUpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPBdGNKSzVyPUCuNVcxOTlizszN NFPOdZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
LAMA-84 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{D0RmlEPTB;MD6xPFA{OiEQvF2= NUe1WolQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
NCI-H1581 MmDuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEG0SpZKSzVyPUCuNVg6PjZizszN MlfHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
A204 NH\oRY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrnWFZKSzVyPUCuNVg6PyEQvF2= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
A549 NFjHZ3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIDWTmVKSzVyPUCuNVk2PSEQvF2= MkL2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
CAL-51 NVvTbWpOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXYTWM2OD1yLkG5OlUyKM7:TR?= NXnlPFBCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
NKM-1 NH\KSm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXpcJg5UUN3ME2wMlIxODJ7IN88US=> NITReok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
LoVo NF;hb|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYjwWVZyUUN3ME2wMlIxOjB{IN88US=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
HAL-01 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MojSTWM2OD1yLkKwNlk4KM7:TR?= Mmr6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
HCC2218 NXj3SWVnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX\pTHV1UUN3ME2wMlIxPjh6IN88US=> MlviQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
BEN NVroc2dNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLBTWM2OD1yLkKxNlk{KM7:TR?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
LC-2-ad MmnlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHQTWM2OD1yLkKyNFg1KM7:TR?= NEjoZlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
LXF-289 MojoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTBwMkWwOFEh|ryP NXjvb5pERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
HGC-27 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7RTWM2OD1yLkK2OFE1KM7:TR?= M1fpbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
LK-2 M4XZbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M32weGlEPTB;MD6yOlgzPCEQvF2= MlP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
CTB-1 NVe4fmdWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFrtbFlKSzVyPUCuNlczOTVizszN M1HCPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
697 M3vWdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTBwMkezOlch|ryP MnXFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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MG-63 M{i2PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVvJR|UxRTBwMke0NFch|ryP NX;KfpgyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
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U251 MnLJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnf3TWM2OD1zNj6zOlc2KM7:TR?= NFfNVZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
TE-9 MnqyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2HKR2lEPTB;MUeuOVE3PiEQvF2= NUPReoxWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
TE-10 NGXzNY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LDTGlEPTB;MUmuNFYxPyEQvF2= M3rDNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
COLO-824 M4TIcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{XHb2lEPTB;MkCuPVI4PiEQvF2= NH3pOJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
HuP-T3 NVzmR456T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzE[ZNKSzVyPUKxMlA4PDhizszN NHuwVnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
KALS-1 Ml;pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\3fG0xUUN3ME2yNU4yPzVizszN M1PK[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK|5USU6JRWK8M4E,
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SW1463 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LtN2lEPTB;M{OuPVg{KM7:TR?= NWnTTZljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
SW1417 M2\H[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkPvTWM2OD1|Nz6wPVUzKM7:TR?= NHLQNIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
NB12 NXS2SY5pT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGL5[pJKSzVyPUO5MlQ3PTVizszN NGPHTZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
NY M37T[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\YTWM2OD12MT6zNlAyKM7:TR?= NFzDb2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
H1048 NHnZ[HdCeG:ydH;zbZMhSXO|YYm= MV21NFAhdk1? MnPhO|IhcA>? M3jZZ45p[W6lZYOgRWJVNTJ4M,MAl4lv\HWlZXSgZZBweHSxc3nz MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd|N{W0Nkc,OjV5M{e1OFI9N2F-
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MOLM13 NXTXRWZNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3vNE4zKM7:TR?= MYG0PEBp NVHNWldW\W6qYX7j[ZMh[2WubDDndo94fGhiaX7obYJqfGmxbjDpcoR2[2WmIHL5JJNmdHWvZYTpcolj NYGzPZNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3N|k{QTNpPkK0O|M6Ozl|PD;hQi=>
MCF7 NVfTWW1zT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1zPO|IxKG6P M4\Ze|czKGh? MlPtSmJU M3m3eZJm\HWlZYOgeIhmKFCHTGCxMY1m\GmjdHXkJIlv[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5 MoHuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4OEiwOFYoRjJ2Nki4NFQ3RC:jPh?=
TamR NHPrUIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFGxZ21KSzVyPUG45qCKdk1? Mlz5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NUewOlkoRjJ2NEW3NFY6RC:jPh?=
MCF7-X  Mor4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnzU|hIUUN3ME2yOEBvVQ>? M1jFb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEW3NFY6Lz5{NES1O|A3QTxxYU6=
T47D-tamR  M1rTdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DqcmlEPTB;MUmgcm0> M2TLNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEW3NFY6Lz5{NES1O|A3QTxxYU6=
293T  NIS4NJdHfW6ldHnvckBCe3OjeR?= MlfINlAhdk1? NYrkbGNMOyCq M4HXV4Rqe3K3cITzJGFsfC2vZXTpZZRm\CCWSV[tTWEhe3SjYnnsbZR6NCC2cnHud4xw[2G2aX;uMEBidmRiYXP0bZZqfHl? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4M{S0PUc,OjR|NkO0OFk9N2F-
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Huh7  NH7rb3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn3KOU0yODBibl2= NVjVXpZ5OC1{NDDo NV;jO|I6TE2VTx?= NXm1eZVicW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ M4jRUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mkm3N|AxLz5{NEK5O|MxODxxYU6=
Hep3B  Mn;JS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVy1MVExOCCwTR?= NEDFTGQxNTJ2IHi= MVvEUXNQ NWXIbZhHcW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ M2\pWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mkm3N|AxLz5{NEK5O|MxODxxYU6=
Huh7  MnLNSpVv[3Srb36gRZN{[Xl? MmjuNVAxKG6P NXnqNYxCOTJiaB?= NGn0fHNFVVOR NETwWoVqdmS3Y3XzJIF2fG:yaHHnfeKh M3G1PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2Mkm3N|AxLz5{NEK5O|MxODxxYU6=
U87-MG  MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;ZO|IhcA>? M1PvXGdKPTEkgJm95qCKPzlibl5CpC=> NH;tZ5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK0PVY1OCd-MkSyOFk3PDB:L3G+
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RMS13 M1nibmFxd3C2b4Ppd{BCe3OjeR?= MmHaNUDPxE1? M3rJelQ5KGh? MV\pcoNz\WG|ZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDBRnQuPzN5 MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|M{KxPEc,OjRzM{OyNVg9N2F-
RH30 NGLyeYFCeG:ydH;zbZMhSXO|YYm= M4mySlEh|ryP MkHtOFghcA>? NHjjdWJqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYOgbY5lfWOnZDDifUBCSlRvN{O3 MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF|M{KxPEc,OjRzM{OyNVg9N2F-
Hep-2 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;R[2dIOC5yMT2xJO69VQ>? MVmxNk06PiCq M4exOolv[3KnYYPld{Bk\WyuIHTlZZRpKGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFyM{e0PUc,OjRzMEO3OFk9N2F-
SCC-9 MkHaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVj1dWlLOC5yMT2xJO69VQ>? M4XiRlEzNTl4IHi= M2P0Solv[3KnYYPld{Bk\WyuIHTlZZRpKGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>? M4PlNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUCzO|Q6Lz5{NEGwN|c1QTxxYU6=
Hep-2 M2fOT2Z2dmO2aX;uJGF{e2G7 NHXlXmYxNjFxMD61JO69VQ>? MnnlNlQhcA>? NVnXNnZM[myxY3vzJI1VV1KFMTDhcoQhdVSRUlOyJIFkfGm4YYTpc44> M{DDZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUCzO|Q6Lz5{NEGwN|c1QTxxYU6=
SCC-9 NFXaW4FHfW6ldHnvckBCe3OjeR?= M2CwT|AvOS9yLkWg{txO M{nINVI1KGh? MlvrZoxw[2u|IH3UU3JEOSCjbnSgcXRQWkN{IHHjeIl3[XSrb36= M2myeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUCzO|Q6Lz5{NEGwN|c1QTxxYU6=
PANC-1 MoiwRZBweHSxc3nzJGF{e2G7 NHTiUFQ2ODBibl2= M2jDdFQ5KGh? NInxUFB{\W6|aYTpfoV{KHCjbnPy[YF1cWNiY3HuZ4VzKGOnbHzzJJRwKHKjZHnveIhmemGyeR?= MlLBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6OE[yPVQoRjJ|OEi2Nlk1RC:jPh?=
RMG2 MmjmRZBweHSxc3nzJGF{e2G7 NELEUIIyNTFyMECgcm0> MYe3NkBp NWjNfolkTkKV NGLGO5NqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJkh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=> Ml3MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MUW2N|EoRjJ|NkG1OlMyRC:jPh?=
HAC2 NEewNWJCeG:ydH;zbZMhSXO|YYm= NUHzdHZDOS1zMECwJI5O NEPhXJM4OiCq NGSxSFBHSlN? NGXZOIpqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJkh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=> NF:2[lg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xOVY{OSd-MkO2NVU3OzF:L3G+
HT-29 NGnx[GRCeG:ydH;zbZMhSXO|YYm= NHXZfWwyKM7:TR?= MUe3NkBp M3\ZfYF1fGWwdXH0[ZMhPS2IVT3pcoR2[2WmIHPlcIwh\GWjdHi= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OUGyNUc,OjF7NEmxNlE9N2F-
DLD1 NEf0RZdCeG:ydH;zbZMhSXO|YYm= MknVNUDPxE1? NVHTO5RxPzJiaB?= M{DLb4F1fGWwdXH0[ZMhPS2IVT3pcoR2[2WmIHPlcIwh\GWjdHi= Mm[4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEmxNlEoRjJzOUS5NVIyRC:jPh?=
HT-29 NWPCO4lpTnWwY4Tpc44hSXO|YYm= M2HRXVEh|ryP NELnO2Q4OiCq NHfHVGdqdmS3Y3XzJIF2fG:yaHHnfUB1dyClb37m[ZIh[3m2b4Dyc5Rm[3Srb36= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OUGyNUc,OjF7NEmxNlE9N2F-
DLD1 NInWVYxHfW6ldHnvckBCe3OjeR?= NVXTNmdWOSEQvF2= MYW3NkBp M2S3W4lv\HWlZYOgZZV1d3CqYXf5JJRwKGOxbn\ldkBkgXSxcILveIVkfGmxbh?= MmfIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEmxNlEoRjJzOUS5NVIyRC:jPh?=
Rh41 NFjENlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTNyLkegcm0> M1nLdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO|Y4QTxxYU6=
Rh18 M{\BOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTRzLkSgcm0> NEnKVms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
Rh30 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTF2IH7N NIHCOFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
BT-12 NF;1cGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfiZWRZUUN3ME2yOE44KG6P MnLrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{e2O|koRjJzM{O3Olc6RC:jPh?=
CHLA-266 NG\wS|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVPNVXVLUUN3ME2xMlEhdk1? MnG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{e2O|koRjJzM{O3Olc6RC:jPh?=
TC-71 MoS2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoC2TWM2OD12Nj61JI5O NFzNSXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
CHLA-9 NV3jNnVyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTF3LkOgcm0> NV\KU4hCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|c3PzlpPkKxN|M4Pjd7PD;hQi=>
CHLA-10 Mke1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLnTWM2OD1|Lkmgcm0> NFHBPZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
CHLA-258 NUXHcFNXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV7JR|UxRTFyLkSgcm0> MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
GBM2 NYW4c4d7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTRyLkegcm0> M4LmZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO|Y4QTxxYU6=
NB-1643 NHPoVVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NED1NWtKSzVyPUOzMlghdk1? NXfpW5h7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|c3PzlpPkKxN|M4Pjd7PD;hQi=>
NB-EBc1 Ml\VS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NY\Me|Q3UUN3ME2yO{4yKG6P MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN|N{[3PUc,OjF|M{e2O|k9N2F-
CHLA-90 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\oTmlEPTB;M{SuOUBvVQ>? NYfpWYh5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|c3PzlpPkKxN|M4Pjd7PD;hQi=>
CHLA-136 NVn1UGxMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\STWM2OD1zNkKuOkBvVQ>? NFjFZYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
NALM-6 MlTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rCOWlEPTB;M{KuPUBvVQ>? NVuz[|FYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|c3PzlpPkKxN|M4Pjd7PD;hQi=>
COG-LL-317 NI[zUGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\GTWM2OD1zLkOgcm0> NGKySnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
RS4;11 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4TaWGlEPTB;M{Wgcm0> NFXQT4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
MOLT-4 Ml\0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M33EdmlEPTB;OD62JI5O M4PY[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO|Y4QTxxYU6=
CCRF-CEM MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLHTWM2OD12LkGgcm0> M1fuUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO|Y4QTxxYU6=
Kasumi-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2fQdmlEPTB;MT60JI5O NXj4VmtKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN|c3PzlpPkKxN|M4Pjd7PD;hQi=>
Karpas-299 M4TaPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4HvZ2lEPTB;MkGuO{BvVQ>? MkHuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|M{e2O|koRjJzM{O3Olc6RC:jPh?=
Ramos-RA1 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2[zWWlEPTB;OD6yJI5O NH7yVXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO|Y4QSd-MkGzN|c3Pzl:L3G+
H838 MUnGeY5kfGmxbjDBd5NigQ>? NGXxNZEyKM7:TR?= M4XJflQ5KGh? NY\yd3FMcW6mdXPld{Bk\WyuIHH1eI9xcGGpeR?= NX7JbFl6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCzOlQyOTNpPkKwN|Y1OTF|PD;hQi=>
H838 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX2wMVMh|ryP Mnq1O|IhcA>? MU\pcoR2[2W|IHfyc5d1cCCrbnjpZol1cW:wIHHu[EBifXSxcHjh[5k> M37oRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEK4PFU1Lz5{MECyPFg2PDxxYU6=
A549  Mlu0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4OzZVAuOyEQvF2= MVK3NkBp MVjpcoR2[2W|IHfyc5d1cCCrbnjpZol1cW:wIHHu[EBifXSxcHjh[5k> NEnZVGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MECyPFg2PCd-MkCwNlg5PTR:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; 

PubMed: 24457069     


Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘瘿삨ՂᾰƌՂĀ㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙

p-EGFR / EGFR; 

PubMed: 25654224     


PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot;

p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; 

PubMed: 25654224     


PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.

24457069 25654224
Immunofluorescence
p-4EBP1 / EIF4E / mTOR; 

PubMed: 24600487     


(C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘瘿삨ՂᾰƌՂĀ㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋€𢡄�෋€䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemセ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒セ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wセ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴￿￿嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗

LC3B / p62 ; 

PubMed: 30337536     


Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞

24600487 30337536
Growth inhibition assay
Cell viability ; 

PubMed: 29263324     


Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.

29263324
体内研究 AZD8055 按2.5/10 mg/kg剂量作用于U87MG和A549移植瘤抑制pS6和pAKT,导致肿瘤生长受抑制。AZD8055按10/20 mg/kg剂量处理多种移植瘤包括U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3和MES-SA时,显示出明显的抗癌活性。[1] AZD8055诱导肿瘤体积下降约40%, 伴随着AKT, S6K,和SGK蛋白激酶磷酸化的切除。用AZD8055 (5mg/kg, 每天2次) 和SAHA (100 mg/kg/每天)处理PTEN+/−LKB1+/hypo 移植瘤,导致肿瘤生长全部受抑制,且对鼠mTORC1和mTORC2信号抑制没有副作用。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

抑制mTOR的细胞为基础的测定实验:

通过高通量筛选细胞检测法,使用MDA-MB-468细胞,测定mTORC1 和mTORC2活性。用增加浓度的AZD8055处理细胞2小时。温育到最后时,细胞进行固定,冲洗,用p-Akt(S473)抗体和p-S6(S235/236)抗体探测。用激光扫描仪检测磷酸化水平。
细胞实验:[1]
- 合并
  • Cell lines: U87MG, A549,和H838 细胞
  • Concentrations: 溶解在DMSO中,作为10 mM 储备液, 0.001-1.0 μM
  • Incubation Time: 72到96小时
  • Method: 用AZD8055处理细胞72到96小时, 用0.03 mg/mL Hoechst 33342染色细胞核,用1 μg/mL 吖啶橙染色酸性小囊泡染色。通过ArrayScan II平台在450和536纳米处获得图像,测量酸性小囊泡的百分数和细胞数量。进行LC3测评,用10 μg/mL e64d/抑肽素处理细胞30 到90分钟,然后和AZD8055温育。细胞溶解在冰上,通过免疫印迹法分析。
    (Only for Reference)
动物实验:[1]
- 合并
  • Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG和A549细胞建立在无病原体,雌性裸鼠体内(nu/nu:Alpk)。
  • Dosages: 2.5-20 mg/kg
  • Administration: 口服饲喂,每天1到2次。
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 50 mg/mL warmed (107.4 mM)
Ethanol 3 mg/mL (6.44 mM)
Water Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
4% DMSO+30% PEG 300+ddH2O
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 465.54
化学式

C25H31N5O4

CAS号 1009298-09-2
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01316809 Completed Drug: AZD8055 Glioblastoma Multiforme|Anaplastic Astrocytoma|Anaplastic Oligodendroglioma|Malignant Glioma|Brainstem Glioma National Cancer Institute (NCI)|National Institutes of Health Clinical Center (CC) March 4 2011 Phase 1
NCT00999882 Completed Drug: AZD8055 Cancer|Advanced Hepatocellular Carcinoma AstraZeneca October 2009 Phase 1
NCT00973076 Completed Drug: AZD8055 Cancer|Solid Tumors|Advanced Solid Malignancies AstraZeneca August 2009 Phase 1
NCT00731263 Completed Drug: AZD8055 Solid Tumors AstraZeneca July 2008 Phase 1
NCT01194193 Withdrawn Drug: AZD8055 Cancer|Advanced Solid Tumours|Lymphomas AstraZeneca Phase 1

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

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