AZD8055

For research use only. Not for use in humans.

目录号：S1555

CAS No. 1009298-09-2

AZD8055是一种新型的，ATP竞争性mTOR抑制剂，在MDA-MB-468 细胞中IC50为0.8 nM，与作用于PI3K亚型和ATM/DNA-PK相比，具有优异的选择性（约1000倍）。AZD8055可诱导半胱天冬酶依赖的凋亡和自噬。Phase 1。

规格

价格

库存

购买数量

10mM (1mL in DMSO)	RMB 1265.49	现货
5mg	RMB 712.53	现货
10mg	RMB 988.31	现货
50mg	RMB 3048.72	现货
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客户使用Selleck生产的AZD8055发表文献180篇：

Nature, 2019, 10.1038/s41586-019-1607-3

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Nat Med, 2019, 25(5):838-849

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Cancer Cell, 2019, 35(5):752-766

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Cell, 2018, 172(1-2):373-386

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Cell Stem Cell, 2018, 22(3):369-383

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Nat Med, 2017, 23(2):200-212

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Cell Stem Cell, 2017, 20(2):177-190

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Cancer Cell, 2017, 31(5):635-652

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Nat Med, 2015, 10.1038/nm.3855

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Cancer Discov, 2015, 5(3):255-63

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Cancer Discov, 2015, 5(3):245-54

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Cancer Cell, 2015, 27(1):97-108

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Cancer Cell, 2015, 27(4):533-46

PubMed: 25873175 ( click the link to review the publication )

Nat Commun, 2020, 11(1):4684

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Dev Cell, 2020, 55(3):298-313.e10

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Clin Cancer Res, 2020, 10.1158/1078-0432

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Cell Rep, 2020, 31(12):107810

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Eur J Cancer, 2020, 126:93-103

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J Bone Miner Res, 2020, 10.1002/jbmr.4176

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J Exp Clin Cancer Res, 2020, 9;39(1):107

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Br J Cancer, 2020, 22

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Front Immunol, 2020, 11:572960

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Mol Cancer Ther, 2020, 5;molcanther.1131.2019

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Mol Cancer Ther, 2020, 19(3):882-894

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Aging (Albany NY), 2020, 12(19):19022-19044

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J Cancer, 2020, 15;11(12):3492-3501

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Sci Rep, 2020, 4;10(1):4025

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J Biol Chem, 2020, 22;295(21):7418-7430

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Cell Cycle, 2020, 1-15

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Biosci Rep, 2020, 30;40(4):BSR20200905

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Biochem Biophys Res Commun, 2020, 23;S0006-291X(20)30951-7

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Nat Commun, 2020, 11(1):3627

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BMC Cancer, 2020, 20(1):724

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Nat Med, 2020, 10.1038/s41591-020-0986-1

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J Cell Biol, 2020, 219(2)

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J Invest Dermatol, 2020, S0022-202X(20)31981-3

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Int Immunopharmacol, 2020, 81:106241

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Cell Rep, 2020, 32(3):107933

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Mol Oncol, 2020, 10.1002/1878-0261.12673

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Cancer, 2020, 10.1002/cncr.33071

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Mol Cell, 2019, 10.1016/j.molcel.2019.10.016

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Autophagy, 2019, 10.1080/15548627.2019.1660545

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Autophagy, 2019, 15(3):543-557

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Clin Cancer Res, 2019, 25(21):6429-6442

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Curr Biol, 2019, 29(5):737-749

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Cell Rep, 2019, 27(12):3696-3708.e5

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Sci Signal, 2019, 12(612)

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Cancers (Basel), 2019, 11(10)

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J Neurosci, 2019, 39(27):5255-5268

PubMed: 31043483 ( click the link to review the publication )

Cell Death Dis, 2019, 10(8):563

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Cell Chem Biol, 2019, 26(3):366-377

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Am J Cancer Res, 2019, 9(9):1922-1937

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Sci Rep, 2019, 9(1):30

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Sci Rep, 2019, 9(1):9805

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Cancer Gene Ther, 2019, 10.1038/s41417-019-0117-7

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Biochem J, 2019, 476(20):3081-3107

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Front Physiol, 2019, 10:167

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Biomed Res Int, 2019, 2019:5196028

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JCI Insight, 2019, 4(20)124164

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Cell Mol Gastroenterol Hepatol, 2019, 8(1):21-36

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Cancer Biol Med, 2019, 16(1):66-83

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Nat Cell Biol, 2018, 20(4):465-478

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Cancer Res, 2018, 78(12):3176-3189

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Cancer Res, 2018, 78(15):4396-4410

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Cell Death Dis, 2018, 9(11):1063

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J Mol Cell Biol, 2018, 10(4):331-340

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Acta Pharmacol Sin, 2018, 39(8):1338-1346

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Front Genet, 2018, 9:690

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Cell Signal, 2018, 44:127-137

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PLoS One, 2018, 13(8):e0200725

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Med Sci Monit, 2018, 24:2735-2743

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Oncol Lett, 2018, 16(4):4561-4566

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J Exp Med, 2018, 215(1):159-175

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Oncol Lett, 2018, 16(1):632-642

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Nat Commun, 2017, 8(1):1090

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Nat Commun, 2017, 8(1):2207

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Nucleic Acids Res, 2017, 45(16):9654-9678

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Cell Syst, 2017,

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Mol Syst Biol, 2017, 13(1):905

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Cell Rep, 2017, 20(10):2341-2356

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Oncogene, 2017, 36(15):2074-2084

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Oncogene, 2017, 36(26):3651-3660

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Sci Signal, 2017, 10(507)eaam7550

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PLoS Pathog, 2017, 13(4):e1006344

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Cell Commun Signal, 2017, 10.1186/s12964-017-0164-3

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Biochim Biophys Acta Mol Cell Res, 2017, 1864(10):1554-1565

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Sci Rep, 2017, 7(1):1994

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PLoS One, 2017, 12(7):e0181243

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Anticancer Res, 2017, 37(3):1083-1090

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Oncotarget, 2017, 8(4):5992-6002

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Oncotarget, 2017, 8(1):979-987

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Oncotarget, 2017, 8(25):41422-41431

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Nat Cell Biol, 2016, 18(5):527-39

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Genome Biol, 2016, 17(1):149

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Nat Chem Biol, 2016, 12(1):22-8

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EMBO J, 2016, 35(17):1902-22

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Mol Oncol, 2016, 10(1):101-12

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Mol Oncol, 2016, 10(4):538-52

PubMed: 26686971 ( click the link to review the publication )

Aging (Albany NY), 2016, 8(2):231-44

PubMed: 26851731 ( click the link to review the publication )

Aging (Albany NY), 2016, 8(12):3535-3551

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Sci Rep, 2016,

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J Biol Chem, 2016, 291(12):6262-71

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Front Plant Sci, 2016, 7:1746

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Cell Signal, 2016, 28(4):284-93

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PLoS One, 2016, 11(4):e0154564

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Biochem Biophys Res Commun, 2016, 478(4):1515-20

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Int J Endocrinol, 2016, 2016:5286972

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Oncotarget, 2016, 7(44):71211-71222

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Oncotarget, 2016, 7(4):3947-65

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Autophagy, 2015, 11(2):285-97

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Cancer Res, 2015, 75(20):4398-406

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Cell Rep, 2015, 11(3):446-59

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J Hematol Oncol, 2015,

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Sci Signal, 2015, 8(375):ra42

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J Med Chem, 2015, 58(16):6559-73

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Cancer Discov, 2015, 5(3):245-54

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Clin Sci, 2015, 128(8):517-26

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J Cancer, 2015, 6(12):1195-205

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Mol Cancer Res, 2015, 13(2):223-30

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Mol Cancer Res, 2015, 13(1):120-9

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Mol Cancer Res, 2015, 13(1):120-9

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Mol Cancer Res, 2015, 13(2):223-30

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Int J Mol Sci, 2015, 16(2):3267-82

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J Biol Chem, 2015, 10.1074/jbc.C114.627778

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Biochem J, 2015, 465(2):227-38

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J Chromatogr A, 2015, 1388:267-73

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J Chromatogr A, 2015, 1388:267-73

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Proteomics, 2015, 10.1002/pmic.201400586

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Cancer Biol Ther, 2015,

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PLoS One, 2015, 10(11):e0143357

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PLoS One, 2015, 10(7):e0131400

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Clin Sci (Lond), 2015, 128(8):517-26

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Oncotarget, 2015, 6(7):5118-33

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Cell Signal, 2015, 27(11):2191-200

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J Clin Invest, 2014, 10.1172/JCI73935

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Nat Commun, 2014, 5:3384

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Autophagy, 2014, 10(6):1120-36

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Leukemia, 2014, 28(5):1092-102

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Cancer Res, 2014, 74(10):2846-56

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Cancer Res, 2014, 74(14):3947-58

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Clin Cancer Res, 2014, 20(22):5756-67

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Mol Cell Biol, 2014, 34(22):4088-103

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Oncol Rep, 2014, 31(2):649-56

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PLoS One, 2014, 9(12):e113555

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Oncotarget, 2014,

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Blood, 2013, 121(18):3619-30

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EMBO J, 2013, 28;32(17):2377-91

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Cancer Res, 2013, 73(11):3402-11

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Mol Cancer, 2013, 12:81

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Mol Cancer Ther, 2013, 13(1):37-48

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Mol Cancer Ther, 2013,

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Mol Oncol, 2013, 33:404-18

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J Hypertens, 2013, 31(2):303-11

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Oncol Lett, 2013, 5(2):717-721

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Autophagy, 2012, 9(1):74-92

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产品安全说明书

mTOR抑制剂选择性比较

生物活性

产品描述

特性

AZD8055是第一种抑制两种类型mTOR蛋白的药物，被认为可能比之前mTOR抑制剂更有效。

靶点

mTOR (truncated) ^[1] (MDA-MB-468 cells)	mTOR (full length) ^[1] (MDA-MB-468 cells)
0.13 nM	0.8 nM

体外研究

AZD8055作用于所有 PI3K亚型 (α, β, γ, δ) 和其他近PI3K激酶家族(ATM和DNA-PK)显示出低活性。AZD8055抑制mTORC1(p70S6K 和4E-BP1) ，mTORC2 (AKT) 和下游蛋白的磷酸化。AZD8055完全抑制4E-BP1抗Rapamycin 的T37/46 磷酸化位点, 导致明显抑制cap-dependent 转译作用。AZD8055有效抑制U87MG, A549和H838 细胞增殖， IC50分别为53, 50，和20 nM。AZD8055作用于H838和A549细胞，也诱导自体吞噬和增加LC3-II 水平。^[1] AZD8055降低白血病细胞增殖和细胞周期进展, 降低白血病祖细胞的无性系生长，且在白血病细胞中诱导caspase依赖的细胞凋亡，但是作用于正常未成熟的CD34+ 细胞时不会产生这种诱导作用。^[2] AZD8055 抑制儿科临床前期测试计划(PPTP)细胞系，IC50 为24.7 nM，且在EFS 分布时诱导产生明显区别。^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
LU-139	NWnzN2ZUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NWPETpV1UUN3ME2wMlAzOzNzIN88US=>	NFi1Z4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
NCI-H720	MnzGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NETJfmNKSzVyPUCuNFI6OzhizszN	NGjqXm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
KARPAS-45	MmXzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NF;OPG1KSzVyPUCuNFM{PzFizszN	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
EW-3	MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MUnJR\|UxRTBwMESwOVYh\|ryP	M2nQblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
SBC-1	NWrnNGNXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M{LKU2lEPTB;MD6wOFU5OSEQvF2=	NGXiWGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
NCI-H209	MkDtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MnrxTWM2OD1yLkC1OFU{KM7:TR?=	MlvJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
EoL-1-cell	NECxbYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NHTNZ3VKSzVyPUCuNFY2PjlizszN	M17YdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
H9	M2C0bWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NUPkTXZ[UUN3ME2wMlA3Pjl{IN88US=>	Ml7OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
AN3-CA	NHjsXXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NEH6T\|lKSzVyPUCuNFc6PTFizszN	MmHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
HH	NGXkbW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M{fyV2lEPTB;MD6wPFAxQSEQvF2=	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
KU812	NEjMelRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUTJR\|UxRTBwMEiwOFMh\|ryP	M{HiNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
DU-4475	MoDES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NILJW2pKSzVyPUCuNFgxPDdizszN	MlLWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
CTV-1	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4jOOWlEPTB;MD6wPFY1OSEQvF2=	NYD1NGNvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
BV-173	NF3mToNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWHJR\|UxRTBwMEmwOlEh\|ryP	M3zDRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
ES5	NGHWdWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1TtdGlEPTB;MD6wPVM2KM7:TR?=	NYnXdZBbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
G-402	NInPfFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUfJR\|UxRTBwMEm5PEDPxE1?	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
MV-4-11	Mn7SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NUTtN5B5UUN3ME2wMlExOzR5IN88US=>	M2exOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
NB69	NH;DbIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NGrvZplKSzVyPUCuNVM2KM7:TR?=	NFTiO489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
EGI-1	NX;GZlY3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MWHJR\|UxRTBwMUO1N\|Eh\|ryP	MnTlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
EW-18	M1S4Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NGKxc2JKSzVyPUCuNVQxPzNizszN	NHPSWII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
ME-180	M{X0XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MYnJR\|UxRTBwMUS2PFEh\|ryP	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
D-283MED	NF7kXGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnvZTWM2OD1yLkG0O\|I{KM7:TR?=	MmDyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
NB14	NXTxdpBnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MmKxTWM2OD1yLkG2OFQ4KM7:TR?=	NHnabZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
Ramos-2G6-4C10	NGPaS3ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M37SWWlEPTB;MD6xOlk5OyEQvF2=	NInKepM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
NBsusSR	Ml;vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MWDJR\|UxRTBwMUewNVkh\|ryP	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
LAMA-84	NITyeGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NGDSRVVKSzVyPUCuNVgxOzJizszN	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
NCI-H1581	MkDpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXXEVWVVUUN3ME2wMlE5QTZ4IN88US=>	M4\hblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
A204	M2rTU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NV3u[WtnUUN3ME2wMlE5QTdizszN	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
A549	MkS1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MmK4TWM2OD1yLkG5OVUh\|ryP	NF3hPGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
CAL-51	M3Hoc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MmTlTWM2OD1yLkG5OlUyKM7:TR?=	M13V[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
NKM-1	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFXvUHBKSzVyPUCuNlAxOjlizszN	Mm\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
LoVo	M{jrcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M{\3d2lEPTB;MD6yNFIxOiEQvF2=	MofRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
HAL-01	NI[yepBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MX\JR\|UxRTBwMkCyPVch\|ryP	NHzXdGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
HCC2218	NVnrN5pOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NV3i[oxNUUN3ME2wMlIxPjh6IN88US=>	M4HFUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
BEN	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M12zTmlEPTB;MD6yNVI6OyEQvF2=	NVPRfpY5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
LC-2-ad	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MWPJR\|UxRTBwMkKwPFQh\|ryP	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
LXF-289	M2O4Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NV3VW3hvUUN3ME2wMlI2ODRzIN88US=>	NWLYNWFoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
HGC-27	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MWrJR\|UxRTBwMk[0NVQh\|ryP	NEHCOmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
LK-2	M2LNV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MnzoTWM2OD1yLkK2PFI1KM7:TR?=	MmX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
CTB-1	NIO0RlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NWO0bZVKUUN3ME2wMlI4OjF3IN88US=>	M3H5OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
697	M4TvRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M2n2NWlEPTB;MD6yO\|M3PyEQvF2=	MoO1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
ES7	M1zEdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NUDxV\|NVUUN3ME2wMlI4Ozh\|IN88US=>	M2LxUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
MG-63	M1\2Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MWPJR\|UxRTBwMke0NFch\|ryP	M{nyT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5ODF{MESvK\|5USU6JRWK8M4E,
T47D	NYDVNmdUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4\vcWlEPTB;MD6yO\|Q4PSEQvF2=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxPFAyOjB2Lze+V2FPT0WUPD;hQi=>
RPMI-8226	MnjxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NX;GOJFVUUN3ME2wMlI5PTh7IN88US=>	MoXEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
NCI-H292	M3j1RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MoPOTWM2OD1yLkK5NVY1KM7:TR?=	NY\QN3UxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
HT	MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MUXJR\|UxRTBwMkmzPEDPxE1?	NHzYNZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
A3-KAW	NFGwPG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MX3JR\|UxRTBwMkm3OEDPxE1?	NYPZ[W5qRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
LB2241-RCC	MkfPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXfJR\|UxRTBwM{CxPVgh\|ryP	Ml3vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
BB65-RCC	Mn6yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MlSyTWM2OD1yLkOwPFA2KM7:TR?=	NYPkboJjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOThyMUKwOE8oRlODTlfFVlww[T5?
G-401	NYfJd\|dLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M{nHfWlEPTB;MD6zNFk5PiEQvF2=	NFKyTJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
TYK-nu	MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NUOyXXRpUUN3ME2wMlMyQDZ2IN88US=>	NGn2ZYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
NCI-SNU-1	NXvMPGhQT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXzYToo6UUN3ME2wMlMzOzNzIN88US=>	NIjoSYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDBzMkC0M{c,W0GQR1XSQE9iRg>?
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OVCAR-5	NGHWWoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MoTyTWM2OD1yLkSyPVM{KM7:TR?=	MnXGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVgxOTJyND:nQnNCVkeHUkyvZV4>
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Hep-2	M16zemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MlTONE4xOS1zIN88US=>	NHHoW4QyOi17NjDo		NWi3dJJTcW6lcnXhd4V{KGOnbHyg[IVifGhiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{	NX3yd3AzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNFM4PDlpPkK0NVA{PzR7PD;hQi=>
SCC-9	NInOeXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NH:yWoQxNjBzLUGg{txO	M{PiOlEzNTl4IHi=		NInaNmlqdmO{ZXHz[ZMh[2WubDDk[YF1cCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ?	Mnj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMEO3OFkoRjJ2MUCzO\|Q6RC:jPh?=
Hep-2	MVHGeY5kfGmxbjDBd5NigQ>?	MWqwMlEwOC53IN88US=>	NHmwN\|gzPCCq		NF\kcVljdG:la4OgcXRQWkNzIHHu[EBuXE:UQ{KgZYN1cX[jdHnvci=>	NGPhZmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGwN\|c1QSd-MkSxNFM4PDl:L3G+
SCC-9	MXrGeY5kfGmxbjDBd5NigQ>?	NGTqemoxNjFxMD61JO69VQ>?	M1f2b\|I1KGh?		NF\NVnNjdG:la4OgcXRQWkNzIHHu[EBuXE:UQ{KgZYN1cX[jdHnvci=>	MmLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMEO3OFkoRjJ2MUCzO\|Q6RC:jPh?=
PANC-1	NXfBXnp5SXCxcITvd4l{KEG\|c3H5	M4L3TlUxOCCwTR?=	NX\YbFJvPDhiaB?=		M4WwdZNmdnOrdHn6[ZMheGGwY4LlZZRq[yClYX7j[ZIh[2WubIOgeI8hemGmaX;0bIVz[XC7	NEPD[mU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i4OlI6PCd-MkO4PFYzQTR:L3G+
RMG2	NF\Ue5NCeG:ydH;zbZMhSXO\|YYm=	Mnf6NU0yODByIH7N	MkWyO\|IhcA>?	NGXQNFFHSlN?	NIrte\|dqdmirYnn0d{B1cGViY3XscEB3cWGkaXzpeJkh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=>	NUT4dJVyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NVU3OzFpPkKzOlE2PjNzPD;hQi=>
HAC2	MlfCRZBweHSxc3nzJGF{e2G7	NXT3VIN3OS1zMECwJI5O	NXXLXZdKPzJiaB?=	NWLzNJFuTkKV	NXnITXI4cW6qaXLpeJMhfGinIHPlcIwhfmmjYnnsbZR6KGOxbnPlcpRz[XSrb36g[IVx\W6mZX70cJk>	NF;qU4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xOVY{OSd-MkO2NVU3OzF:L3G+
HT-29	NFrie\|dCeG:ydH;zbZMhSXO\|YYm=	MmrlNUDPxE1?	NH\IOXc4OiCq		MnTBZZR1\W63YYTld{A2NU[XLXnu[JVk\WRiY3XscEBl\WG2aB?=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OUGyNUc,OjF7NEmxNlE9N2F-
DLD1	NHm1cY1CeG:ydH;zbZMhSXO\|YYm=	NHPwVnYyKM7:TR?=	Ml31O\|IhcA>?		NVPmWXhX[XS2ZX71ZZRmeyB3LV\VMYlv\HWlZXSgZ4VtdCCmZXH0bC=>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl2OUGyNUc,OjF7NEmxNlE9N2F-
HT-29	NH:4e3JHfW6ldHnvckBCe3OjeR?=	NXTXVHpiOSEQvF2=	NIf3fng4OiCq		MXTpcoR2[2W\|IHH1eI9xcGGpeTD0c{Bkd26oZYKgZ5l1d3C{b4TlZ5Rqd25?	M2\tOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUS5NVIyLz5{MUm0PVEzOTxxYU6=
DLD1	NVToPVY1TnWwY4Tpc44hSXO\|YYm=	NIHtUIwyKM7:TR?=	Mk\XO\|IhcA>?		M4jKUYlv\HWlZYOgZZV1d3CqYXf5JJRwKGOxbn\ldkBkgXSxcILveIVkfGmxbh?=	Mm\qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7NEmxNlEoRjJzOUS5NVIyRC:jPh?=
Rh41	M3raUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M13pfmlEPTB;M{CuO{BvVQ>?	MmfxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|M{e2O\|koRjJzM{O3Olc6RC:jPh?=
Rh18	NX;4eGl3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1mxOWlEPTB;NEGuOEBvVQ>?	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN\|N{[3PUc,OjF\|M{e2O\|k9N2F-
Rh30	NVT0bm5HT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M1fmc2lEPTB;MUSgcm0>	NV7UN3kzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
BT-12	MnSwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3Xre2lEPTB;MkSuO{BvVQ>?	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN\|N{[3PUc,OjF\|M{e2O\|k9N2F-
CHLA-266	NXH2bHkyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NXXqVol7UUN3ME2xMlEhdk1?	NXHKTZdyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
TC-71	NI\iNYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2XqR2lEPTB;NE[uOUBvVQ>?	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN\|N{[3PUc,OjF\|M{e2O\|k9N2F-
CHLA-9	NI\PcXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M1zvWmlEPTB;MUWuN{BvVQ>?	NF\lZ4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
CHLA-10	M4XJNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NV74THM{UUN3ME2zMlkhdk1?	MmHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|M{e2O\|koRjJzM{O3Olc6RC:jPh?=
CHLA-258	NGf6SW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MVvJR\|UxRTFyLkSgcm0>	M1XTdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO\|Y4QTxxYU6=
GBM2	M2DLZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1\iT2lEPTB;NECuO{BvVQ>?	NHS0SGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
NB-1643	MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NH7IOFhKSzVyPUOzMlghdk1?	NWK4fZp5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
NB-EBc1	Mm\xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NETXfGVKSzVyPUK3MlEhdk1?	M4r2e\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO\|Y4QTxxYU6=
CHLA-90	NWHaW3JyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M4XZdGlEPTB;M{SuOUBvVQ>?	M{XHTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO\|Y4QTxxYU6=
CHLA-136	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NHnSc4JKSzVyPUG2Nk43KG6P	NGnPdHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOzO\|Y4QSd-MkGzN\|c3Pzl:L3G+
NALM-6	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MXXJR\|UxRTN{Lkmgcm0>	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN\|N{[3PUc,OjF\|M{e2O\|k9N2F-
COG-LL-317	M{LLT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NV62UVg3UUN3ME2xMlMhdk1?	NVjCVm11RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
RS4;11	NFP1[5ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NFHpW4tKSzVyPUO1JI5O	NXq0TIFVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
MOLT-4	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NXvwU3JyUUN3ME24MlYhdk1?	MlzEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF\|M{e2O\|koRjJzM{O3Olc6RC:jPh?=
CCRF-CEM	NI\3OWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MX7JR\|UxRTRwMTDuUS=>	M4i2VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{O3Olc6Lz5{MUOzO\|Y4QTxxYU6=
Kasumi-1	NEPQT2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MnXrTWM2OD1zLkSgcm0>	NUDKeoppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
Karpas-299	M3rDVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MojJTWM2OD1{MT63JI5O	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN\|N{[3PUc,OjF\|M{e2O\|k9N2F-
Ramos-RA1	NFO0fndIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmTzTWM2OD16LkKgcm0>	NUHSWWhTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzN\|c3PzlpPkKxN\|M4Pjd7PD;hQi=>
H838	M3voemZ2dmO2aX;uJGF{e2G7	MmHKNUDPxE1?	NF;Tbng1QCCq		NYnpemJTcW6mdXPld{Bk\WyuIHH1eI9xcGGpeR?=	M2fUelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyM{[0NVE{Lz5{MEO2OFEyOzxxYU6=
H838	M{XIPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Ml;INE0{KM7:TR?=	MVe3NkBp		MojabY5lfWOnczDndo94fGhiaX7obYJqfGmxbjDhcoQh[XW2b4DoZYd6	MmX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjByMki4OVQoRjJyMEK4PFU1RC:jPh?=
A549	MmrGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MX:wMVMh\|ryP	M1\KNlczKGh?		MUHpcoR2[2W\|IHfyc5d1cCCrbnjpZol1cW:wIHHu[EBifXSxcHjh[5k>	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODB{OEi1OEc,OjByMki4OVQ9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	p-mTOR / p-AKT / p-4EBP1 / p-P70S6K / p-PRAS40; PubMed: 24457069 Breast Cancer Res Western blots of 70% confluent TamR (A) and MCF7-X (B) cells treated for 15 minutes to 24 hours with AZD8055 (0 to 100 nM). Blots were probed with phospho- and total antibodies for mTORC1 and two signalling pathways. In both cell lines mTORC1 and mTORC2 s䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙 p-EGFR / EGFR; PubMed: 25654224 Int J Mol Sci PANC-1 cells were treated with AZD8055 for the indicated hours and EGFR (T/P), AKT (T/P) and ribosomal protein S6 (S6) (T/P) proteins were examined by western-blot; p-FOXO1 / FOXO1 / p-FOXO3a / FOXO3a ; PubMed: 25654224 Int J Mol Sci PANC-1 cells were transfected with FoxO1/FoxO3a siRNAs and treated with AZD8055 for 1 h, then AKT (T/P), FoxO1 (T/P), FoxO3a (T/P) and EGFR (T/P) proteins were examined by western-blot.	24457069 25654224
Immunofluorescence	p-4EBP1 / EIF4E / mTOR; PubMed: 24600487 Int J Clin Exp Med (C) Hoechst 33258 staining of Hep-2 cells after treatment with AZD8055 for 24 hours were shown. The cells were fixed and immunereacted with anti-mTOR antibody followed by incubation with Alexa 488-labeled secondary antibody (C-F). Images were taken by a c䲧疝Ỵ疞㧀疜膉痘 瘿삨ՂᾰƌՂĀ 㺣痖帉痖Ѐ瑖堘𢡄빢᎒ՂĀ鑸᎒彿堙奋堙巫堙᎒ﻺ᎒彿堙ﻮ᎒塚堙ﻺ᎒ꍈ堞빢᎒學堙漸堞圔堙빢᎒圞堙圭堙𢡄玚Wᾰƌ ᾰƌ戤瘯Ɖ�෋䐺痖暼瘿�෋ᾰƌ �෋à㺣痖�෋𢡄�෋䀷痗�෋౴�෋㵶痗�෋뺖᎒泌Itemｾ᎒Count﫨呂�෋猴፲�෋፲씢痗猸፲髸莤䥷堙᎒ｾ᎒�堞ﻮ᎒፲露𢡄堚،齃礤v�堞ﻮ᎒猢Wｾ᎒䨼Ą鹿齃殺獸W戀醙玛W戀ⶰ໯齃礤v 玚W玢Wⶰ໯﫰礌vtЉᔈැ鲬౴᥊疜犈፲Eo狜፲艘ᎂ艘ᎂ⩈̎䥠̌씢痗狠፲붖᎒i變ﱘ﫰﬐먙堘붖᎒狠፲i變ﱘ﫰먰堘ﱘ붖᎒�堞봆᎒릎堘붖᎒i꣩쯟巰痗ꣵ쯟巰痗Љ镴౴嶴痗Љ镨౴ᔈැ竗ᔈැ屷痗W竗尊痗 LC3B / p62 ; PubMed: 30337536 Cell Death Dis Immunofluorescence staining of U87 and U251 GICs, which treated by DMSO, AZD8055 (0.3 μM) and rapamycin (3 μM). The nuclei were stained with DAPI and the antibody against LC3B and p62. Images were captured by laser confocal microscope ( × 400), scale bar 䲧疝Ỵ疞	24600487 30337536
Growth inhibition assay	Cell viability ; PubMed: 29263324 Nat Commun Growth for the indicated cell lines was assessed after 72 h of treatment with AZD8055 (0–250 nM). Results are expressed as a percentage of cell number relative to the DMSO-treated control cells a.	29263324

体内研究

AZD8055 按2.5/10 mg/kg剂量作用于U87MG和A549移植瘤抑制pS6和pAKT，导致肿瘤生长受抑制。AZD8055按10/20 mg/kg剂量处理多种移植瘤包括U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3和MES-SA时，显示出明显的抗癌活性。^[1] AZD8055诱导肿瘤体积下降约40%, 伴随着AKT, S6K，和SGK蛋白激酶磷酸化的切除。用AZD8055 (5mg/kg, 每天2次) 和SAHA (100 mg/kg/每天)处理PTEN+/−LKB1+/hypo 移植瘤，导致肿瘤生长全部受抑制，且对鼠mTORC1和mTORC2信号抑制没有副作用。^[4]

激酶实验：^[1]	- 合并抑制mTOR的细胞为基础的测定实验: 通过高通量筛选细胞检测法，使用MDA-MB-468细胞，测定mTORC1 和mTORC2活性。用增加浓度的AZD8055处理细胞2小时。温育到最后时，细胞进行固定，冲洗，用p-Akt（S473）抗体和p-S6（S235/236)抗体探测。用激光扫描仪检测磷酸化水平。
细胞实验：^[1]	- 合并 Cell lines: U87MG, A549，和H838 细胞 Concentrations: 溶解在DMSO中，作为10 mM 储备液, 0.001-1.0 μM Incubation Time: 72到96小时 Method: 用AZD8055处理细胞72到96小时，用0.03 mg/mL Hoechst 33342染色细胞核，用1 μg/mL 吖啶橙染色酸性小囊泡染色。通过ArrayScan II平台在450和536纳米处获得图像，测量酸性小囊泡的百分数和细胞数量。进行LC3测评，用10 μg/mL e64d/抑肽素处理细胞30 到90分钟，然后和AZD8055温育。细胞溶解在冰上，通过免疫印迹法分析。 (Only for Reference)
动物实验：^[1]	- 合并 Animal Models: U87MG, BT474c, A549, Calu-3, LoVo, SW620, PC3 and MES-SA U87-MG和A549细胞建立在无病原体，雌性裸鼠体内(nu/nu:Alpk)。 Dosages: 2.5-20 mg/kg Administration: 口服饲喂，每天1到2次。 (Only for Reference)

参考文献

[4] García-Martínez JM, et al. Br J Cancer, 2011, 104(7), 1116-1

- 合并

溶解度 (25°C)

体外	DMSO	50 mg/mL warmed (107.4 mM)
	Ethanol	3 mg/mL (6.44 mM)
	Water	Insoluble
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 4% DMSO+30% PEG 300+ddH2O	5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download AZD8055 SDF

分子量	465.54
化学式	C₂₅H₃₁N₅O₄
CAS号	1009298-09-2
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	N/A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

选择性强的mTOR抑制剂

Rapamycin (Sirolimus) : mTORC1-selective, IC50=~0.1 nM.
活性最高的mTOR抑制剂

Rapamycin (Sirolimus) : mTOR, IC50=~0.1 nM.
FDA批准的mTOR抑制剂

Temsirolimus (CCI-779, NSC 683864) : Approved by FDA for Renal Cell Carcinoma (RCC).
最新的mTOR抑制剂

XL388 ^New : Highly potent, selective, ATP-competitive inhibitor of mTOR with IC50 of 9.9 nM, 1000-fold selectivity over the closely related PI3K kinases.

研究领域

产品类型

定制您的化合物库

AZD8055

客户使用Selleck生产的AZD8055发表文献180篇：

产品安全说明书

mTOR抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download AZD8055 SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

技术支持

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

相关mTOR产品