Torin 2

For research use only. Not for use in humans.

目录号:S2817

Torin 2 Chemical Structure

CAS No. 1223001-51-1

Torin 2 是一种有效的选择性mTOR抑制剂,在p53−/− MEFs细胞系中IC50为0.25 nM;作用于mTOR比作用于PI3K选择性高800倍,并且改善了药代动力学性能。抑制ATM/ATR/DNA-PK,在PC3细胞系中EC50分别为28 nM/35 nM/118 nM。Torin 2 可降低细胞活力并诱导自噬与凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1562.81 现货
RMB 903.84 现货
RMB 3030.61 现货
RMB 4671.89 现货
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客户使用Selleck生产的Torin 2发表文献49篇:

产品安全说明书

mTOR抑制剂选择性比较

生物活性

产品描述 Torin 2 是一种有效的选择性mTOR抑制剂,在p53−/− MEFs细胞系中IC50为0.25 nM;作用于mTOR比作用于PI3K选择性高800倍,并且改善了药代动力学性能。抑制ATM/ATR/DNA-PK,在PC3细胞系中EC50分别为28 nM/35 nM/118 nM。Torin 2 可降低细胞活力并诱导自噬与凋亡。
靶点
mTOR [1]
(p53−/− MEFs)
ATM [5]
(PC3 cells)
ATR [5]
(PC3 cells)
DNA-PK [5]
(PC3 cells)
0.25 nM 28 nM(EC50) 35 nM(EC50) 118 nM(EC50)
体外研究

Torin 2与PI3Kγ具有相同的结合模式,V882作为铰链结合点,在内部疏水袋Y867,D841 和D964再提供三个作用于氨基比林侧链的氢键,类似于mTOR的Y2225,D2195 和D2357。[1] Torin 2抑制mTORC1,因此通过促进其核转运激活TFEB,EC50为1.666 mM。[2] Torin 2(< 50 nM)引起MZ-CRC-1和TT细胞的活性显著减少。Torin 2 (100 nM)使MZ-CRC-1和TT细胞的迁移显著减少。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT116 Mni2SpVv[3Srb36gZZN{[Xl? NGfi[VYyKGi{ M4XzTGlvcGmkaYTpc44hd2ZibWTPVmMyKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iVEO4PUBxcG:|cHjvdplt[XSrb36gc44hWlCVNlvCNUBi\nSncjCxJIhzKGK7IGfld5Rmem5iYnzveEBidmGueYPpd{whTUN3ME2wMlAxODJ3zszN MmnGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MUG0PFAoRjJ7MkGxOFgxRC:jPh?=
MOLM13 NYnZN3lTSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M{DLb2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV;MUVE{KGOnbHzzJIJ6KEOnbHytWIl1\XJiR3zvJIF{e2G7LDDJR|UxRDBwMECx{txO NFz1fnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO3NFc3Pid-M{CzO|A4PjZ:L3G+
MOLM14 MYXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MnPjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPT1zNNVQh[2WubIOgZpkhS2WubD3UbZRmeiCJbH:gZZN{[XluIFnDOVA9OC5yMEJOwG0> Mk\MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB|N{C3OlYoRjNyM{ewO|Y3RC:jPh?=
MV4-11 MVjBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MXzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2YND2xNUBk\WyuczDifUBE\WyuLWTpeIVzKEeubzDhd5NigSxiSVO1NFwxNjByMd88US=> NILHUok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO3NFc3Pid-M{CzO|A4PjZ:L3G+
HEK293T M2L4V2Z2dmO2aX;uJIF{e2G7 NVPkPYt7OjBibXnudy=> NXfpUmhxUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHscJkhTkyDRz30ZYdo\WRibWTPVmMyKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT|I6O1RiY3XscJMhfXOrbnegV|ZMOSCxcjDBb5QyKGG|IIP1ZpN1emG2ZTDh[pRmeiB{MDDtbY5{KGK7IHntcZVvd2Kub4T0bY5oKGG|c3H5MEBKSzVyPECuNFHPxE1? MmfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MUG0PFAoRjJ7MkGxOFgxRC:jPh?=
HeLa M1W5R2Z2dmO2aX;uJIF{e2G7 M1;5NFIxKG2rboO= NXH0VlFrUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHscJkhTkyDRz30ZYdo\WRibWTPVmMzKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKEinTHGgZ4VtdHNidYPpcochWz[NMTDvdkBCc3RzIHHzJJN2[nO2cnH0[UBi\nSncjCyNEBucW6|IHL5JIludXWwb3Lsc5R1cW6pIHHzd4F6NCCLQ{WwQFAvODIQvF2= MmHCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MUG0PFAoRjJ7MkGxOFgxRC:jPh?=
RD MX;BcpRqfmm{YXygZZN{[Xl? MWS0JIRigXN? MYTBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDFcpRmem:4aYL1d{BCPzFiaX7m[YN1\WRiaX6gbJVu[W5iUlSgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKH[rcoXzMYlv\HWlZXSgZ4VtdCCmZXH0bEBqdmO3YnH0[YQh\m:{IESg[IF6eyCkeTDD[YxtXGm2ZYKtS4xwKGy3bXnu[ZNk\W6lZTDhd5NigSxiSVO1NF0xNjBzzszN MkLrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzFyOEK3OlQoRjNzMEiyO|Y1RC:jPh?=
HCT116 NUDKdINqTnWwY4Tpc44h[XO|YYm= NVPaXlRqOSCqch?= M4Hwe2lvcGmkaYTpc44hd2ZiQWTNJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDh[pRmeiBzIHjyJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrczygSWM2OD1yLkCyPO69VQ>? NV;HRpFqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNVE1QDBpPkK5NlEyPDhyPD;hQi=>
HCT116 M{XF[2Z2dmO2aX;uJIF{e2G7 MWOxJIhz NUmyd2d2UW6qaXLpeIlwdiCxZjDBWHIhcW5iaIXtZY4hUEOWMUG2JINmdGy|IHHzd4V{e2WmIHHzJIFnfGW{IEGgbJIh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nzMEBGSzVyPUCuNFM2|ryP NVfzW4hsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNVE1QDBpPkK5NlEyPDhyPD;hQi=>
RD MWDDfZRwfG:6aXPpeJkh[XO|YYm= MVm0JIRigXN? NWTKfpl{S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWkRiY3XscJMhcW6ldXLheIVlKG[xcjC0JIRigXNiYomgR4VtdFSrdHXyMWdtdyCudX3pcoV{[2WwY3WgZZN{[XluIFPDOVA:OC5yNN88US=> MnzwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzFyOEK3OlQoRjNzMEiyO|Y1RC:jPh?=
HCT116 NEHqTmRHfW6ldHnvckBie3OjeR?= MkLBNUBpeg>? MmPHTY5pcWKrdHnvckBw\iCGTlHQT{BqdiCqdX3hckBJS1RzMU[gZ4VtdHNiYYPz[ZN{\WRiYYOgZYZ1\XJiMTDodkBjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYOsJGVEPTB;MD6xNVjPxE1? MnLjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MUG0PFAoRjJ7MkGxOFgxRC:jPh?=
PC3 MUHGeY5kfGmxbjDhd5NigQ>? NGe2bXlKdmirYnn0bY9vKG:oIGDJN2tidHCqYTDpckBpfW2jbjDQR|Mh[2WubIOg[ZhxemW|c3nu[{BCc3RzIGO0O|NFKG23dHHueEBie3Onc4Pl[EBieyCyaH;zdIhwenmuYYTpc44hd2ZiQXv0JHRpejNyODDifUBqdW23bn;icI91fGmwZzygSWM2OD1yLkNOwG0> NUKySlZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzNlI2PjZpPkKxN|IzPTZ4PD;hQi=>
TC32 NF3XWJZyUFSVIHHzd4F6 NYXl[VJoeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGTDN|Ih[2WubIO= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
DAOY M{PiVJFJXFNiYYPzZZk> NEfYWFhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiRFHPXUBk\Wyucx?= M3m5ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 NHPRSVhyUFSVIHHzd4F6 MX3xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= NF[yUW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
A673 NH;4bmxyUFSVIHHzd4F6 MULxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? NGTz[2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC MVfxTHRUKGG|c3H5 M2DqRpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz M4\OW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 NXTZUll2eUiWUzDhd5NigQ>? MYPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHN? NH7PUGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 M{j5WJFJXFNiYYPzZZk> M3TERpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQj3FRoMyKGOnbHzz NYO4SZZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
U-2 OS Mn3ydWhVWyCjc4PhfS=> MnL4dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFVvMjDPV{Bk\Wyucx?= NXLES2hvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 MXzxTHRUKGG|c3H5 Mn\NdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOjb4OtNkBk\Wyucx?= MoraQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 NXHB[mVPeUiWUzDhd5NigQ>? MYXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| MnzqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 MlrkdWhVWyCjc4PhfS=> MWXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? NEfNU2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 NHzRWpdyUFSVIHHzd4F6 NIn5NHZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO= MnXjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh18 NYTKd|R7eUiWUzDhd5NigQ>? MWDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? NYXxO4kxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
OHS-50 NXPMdplLeUiWUzDhd5NigQ>? MYPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| MmPhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
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MG 63 (6-TG R) NF7XbXByUFSVIHHzd4F6 NYXaXVNmeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF3HJFY{KCh4LWTHJHIqKGOnbHzz NF3QWXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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SJ-GBM2 NVvRfIlQeUiWUzDhd5NigQ>? NEXFSotyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUi2JQl2yJINmdGy| NXW2TpVIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-37 M4rabpFJXFNiYYPzZZk> MU\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDCWE0{PyClZXzsdy=> NYPMbFRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
TC32 M1W1[pFJXFNiYYPzZZk> MofsdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsdy=> NULnZotZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
MG 63 (6-TG R) NGrpW2lyUFSVIHHzd4F6 MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDNS{A3OyBqNj3US{BTMSClZXzsdy=> MmrkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
U-2 OS MW\xTHRUKGG|c3H5 M3jENpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHUuOiCRUzDj[Yxtew>? MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
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Saos-2 NFXyXZZyUFSVIHHzd4F6 NVTPfldkeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy| M1WyelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 M360WpFJXFNiYYPzZZk> NXH0S2EyeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhV0iVLUWwJINmdGy| NX\xUndiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-S6K / S6K / p-AKT / AKT / IRS1 / p-4EBP1 / 4EBP1 / p-GSK3; 

PubMed: 23436801     


Torin2 is a potent mTOR inhibitor in HCT-116 cells

p-Chk1 / Chk1 / p-Chk2 / Chk2 / p-DNA-PK ; 

PubMed: 23436801     


Torin2 inhibits ATR (UV radiation), ATM and DNA-PK (ionizing radiation) strongly.

23436801
Immunofluorescence
LC3B ; 

PubMed: 23436801     


Hela cells were treated with indicated concentrations of Torin2 for 3 days stained with antibody specific for LC3B and DAPI. 

23436801
Growth inhibition assay
Cell viability; 

PubMed: 24434750     


Concentration–response curves of Torin 2, Torin 1 and WWH030 against gametocytes measured in the high throughout viability assay. Torin 1 was much weaker in potency compared to Torin 2.

24434750
体内研究

在小鼠肝微粒体稳定性试验中,Torin 2表现出>95%的药效学响应,和11.7分钟的半衰期。Torin 2静脉内或口服给药后,在雄性Swiss albino小鼠体内表现出最好的生物利用度(51%),短 的半衰期(0.72小时)和低清除率(19.6 mL/min/kg)。[1] 在Th-MYCN 小鼠体内,Torin 2(20mg/kg)消除MYCN肿瘤,减少MYCN蛋白质水平,并诱导细胞凋亡。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

mTOR 和 PI3K 细胞试验:

对mTOR的细胞IC50值使用p53−/− MEFs测定。细胞用载体或逐渐增减浓度的Torin 2处理1小时,然后裂解。S6K1 Thr-389的磷酸化使用磷酸-特异性抗体进行免疫印迹监测。同时,对PI3Ka的细胞IC50值,在p53−/−/mLST8−/−MEFs或表达S473D突变体Akt1的人PC3细胞中,基于Akt Thr-308的磷酸化进行测定。
细胞实验:

[3]

- 合并
  • Cell lines: MZ-CRC-1 和 TT 细胞
  • Concentrations: 50 nM
  • Incubation Time: 3 天或 5 天
  • Method:

    对于活性测定,MZ-CRC-1和TT细胞以一式四份接种在96孔板(1.0×104细胞/孔),培养基分别包含2.5%和4% FBS。24小时后,细胞用Torin 2处理。在指定时间点,细胞与10 μL CellTiter96 AQueous One溶液在100 μL培养基中培育3小时,吸光度在490 nm下测量。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 雄性 Swiss albino 小鼠
  • Dosages: 20 mg/kg
  • Administration: 静脉内或口服给药
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 20 mg/mL (46.25 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
1.5 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 432.4
化学式

C24H15F3N4O

CAS号 1223001-51-1
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
免费分装抑制剂

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID