Torin 2

For research use only. Not for use in humans.

目录号:S2817

Torin 2 Chemical Structure

CAS No. 1223001-51-1

Torin 2 是一种有效的选择性mTOR抑制剂,在p53−/− MEFs细胞系中IC50为0.25 nM;作用于mTOR比作用于PI3K选择性高800倍,并且改善了药代动力学性能。抑制ATM/ATR/DNA-PK,在PC3细胞系中EC50分别为28 nM/35 nM/118 nM。Torin 2 可降低细胞活力并诱导自噬与凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1562.81 现货
RMB 903.84 现货
RMB 1393.67 现货
RMB 3030.61 现货
RMB 4671.89 现货
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客户使用Selleck生产的Torin 2发表文献47篇:

产品安全说明书

mTOR抑制剂选择性比较

生物活性

产品描述 Torin 2 是一种有效的选择性mTOR抑制剂,在p53−/− MEFs细胞系中IC50为0.25 nM;作用于mTOR比作用于PI3K选择性高800倍,并且改善了药代动力学性能。抑制ATM/ATR/DNA-PK,在PC3细胞系中EC50分别为28 nM/35 nM/118 nM。Torin 2 可降低细胞活力并诱导自噬与凋亡。
靶点
mTOR [1]
(p53−/− MEFs)
ATM [5]
(PC3 cells)
ATR [5]
(PC3 cells)
DNA-PK [5]
(PC3 cells)
0.25 nM 28 nM(EC50) 35 nM(EC50) 118 nM(EC50)
体外研究

Torin 2与PI3Kγ具有相同的结合模式,V882作为铰链结合点,在内部疏水袋Y867,D841 和D964再提供三个作用于氨基比林侧链的氢键,类似于mTOR的Y2225,D2195 和D2357。[1] Torin 2抑制mTORC1,因此通过促进其核转运激活TFEB,EC50为1.666 mM。[2] Torin 2(< 50 nM)引起MZ-CRC-1和TT细胞的活性显著减少。Torin 2 (100 nM)使MZ-CRC-1和TT细胞的迁移显著减少。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT116 NVnM[2tjTnWwY4Tpc44h[XO|YYm= NUj2NGRyOSCqch?= M3TnPGlvcGmkaYTpc44hd2ZibWTPVmMyKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iVEO4PUBxcG:|cHjvdplt[XSrb36gc44hWlCVNlvCNUBi\nSncjCxJIhzKGK7IGfld5Rmem5iYnzveEBidmGueYPpd{whTUN3ME2wMlAxODJ3zszN NUm1S2ZzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNVE1QDBpPkK5NlEyPDhyPD;hQi=>
MOLM13 MlToRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M{ftfmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV;MUVE{KGOnbHzzJIJ6KEOnbHytWIl1\XJiR3zvJIF{e2G7LDDJR|UxRDBwMECx{txO NFnEV|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO3NFc3Pid-M{CzO|A4PjZ:L3G+
MOLM14 MWDBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M4L2TmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV;MUVE1KGOnbHzzJIJ6KEOnbHytWIl1\XJiR3zvJIF{e2G7LDDJR|UxRDBwMECx{txO NFjwV5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO3NFc3Pid-M{CzO|A4PjZ:L3G+
MV4-11 MYXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2YND2xNUBk\WyuczDifUBE\WyuLWTpeIVzKEeubzDhd5NigSxiSVO1NFwxNjByMd88US=> M2DRVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyM{ewO|Y3Lz5|MEO3NFc3PjxxYU6=
HEK293T NX7MWFFNTnWwY4Tpc44h[XO|YYm= NIGxc3IzOCCvaX7z MYHJcohq[mm2aX;uJI9nKE5vdHXycYlv[WyueTDGUGFINXSjZ3fl[EBuXE:UQ{GgLJVvc26xd36gc5Jq\2mwKTDlfJBz\XO|ZXSgbY4hUEWNMkmzWEBk\WyuczD1d4lv\yCVNluxJI9zKEGtdEGgZZMhe3Wkc4TyZZRmKGGodHXyJFIxKG2rboOgZpkhcW2vdX7vZoxwfHSrbnegZZN{[XluIFnDOVA9OC5yMd88US=> NYG3bZMyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNVE1QDBpPkK5NlEyPDhyPD;hQi=>
HeLa MX;GeY5kfGmxbjDhd5NigQ>? MmTWNlAhdWmwcx?= NGj0UYtKdmirYnn0bY9vKG:oIF6teIVzdWmwYXzsfUBHVEGJLYTh[4dm\CCvVF;SR|IhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWOYTDj[YxteyC3c3nu[{BUPktzIH;yJGFsfDFiYYOgd5Vje3S{YYTlJIFnfGW{IEKwJI1qdnNiYomgbY1ufW6xYnzveJRqdmdiYYPzZZktKEmFNUC8NE4xOc7:TR?= M{HqWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MkGxOFgxLz5{OUKxNVQ5ODxxYU6=
RD NFHnWlRCdnSrdnnyZYwh[XO|YYm= NEe4ZoE1KGSjeYO= NFHJcVRCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBGdnSncn;2bZJ2eyCDN{GgbY5n\WO2ZXSgbY4hcHWvYX6gVmQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwII\pdpV{NWmwZIXj[YQh[2WubDDk[YF1cCCrbnP1ZoF1\WRiZn;yJFQh\GG7czDifUBE\WyuVHn0[ZIuT2yxIHz1cYlv\XOlZX7j[UBie3OjeTygTWM2OD1yLkCx{txO MnG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzFyOEK3OlQoRjNzMEiyO|Y1RC:jPh?=
HCT116 M3\6fWZ2dmO2aX;uJIF{e2G7 M17wXVEhcHJ? NGHLU2NKdmirYnn0bY9vKG:oIFHUUUBqdiCqdX3hckBJS1RzMU[gZ4VtdHNiYYPz[ZN{\WRiYYOgZYZ1\XJiMTDodkBjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYOsJGVEPTB;MD6wNljPxE1? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTJzMUS4NEc,Ojl{MUG0PFA9N2F-
HCT116 M3W3TGZ2dmO2aX;uJIF{e2G7 NEe2TmsyKGi{ M2HaTmlvcGmkaYTpc44hd2ZiQWTSJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDh[pRmeiBzIHjyJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrczygSWM2OD1yLkCzOe69VQ>? NEnVb5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUKxNVQ5OCd-MkmyNVE1QDB:L3G+
RD NELieG1EgXSxdH;4bYNqfHliYYPzZZk> NHnOe2Q1KGSjeYO= Mk\0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gVmQh[2WubIOgbY5kfWKjdHXkJIZweiB2IHThfZMh[nliQ3XscHRqfGW{LVfsc{BtfW2rbnXzZ4Vv[2ViYYPzZZktKEOFNUC9NE4xPM7:TR?= NU[ydmJnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{GwPFI4PjRpPkOxNFgzPzZ2PD;hQi=>
HCT116 MmrTSpVv[3Srb36gZZN{[Xl? MlLJNUBpeg>? MWnJcohq[mm2aX;uJI9nKESQQWDLJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDh[pRmeiBzIHjyJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrczygSWM2OD1yLkGxPO69VQ>? NUPETWdIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNVE1QDBpPkK5NlEyPDhyPD;hQi=>
PC3 M2jGV2Z2dmO2aX;uJIF{e2G7 NH3FT2tKdmirYnn0bY9vKG:oIGDJN2tidHCqYTDpckBpfW2jbjDQR|Mh[2WubIOg[ZhxemW|c3nu[{BCc3RzIGO0O|NFKG23dHHueEBie3Onc4Pl[EBieyCyaH;zdIhwenmuYYTpc44hd2ZiQXv0JHRpejNyODDifUBqdW23bn;icI91fGmwZzygSWM2OD1yLkNOwG0> NETPeZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOyNlU3Pid-MkGzNlI2PjZ:L3G+
TC32 NX62V2N{eUiWUzDhd5NigQ>? M{jtepFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCWQ{OyJINmdGy| Mme4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
DAOY M2LmUpFJXFNiYYPzZZk> MljVdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKESDT2mgZ4VtdHN? NVSzdGZLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SJ-GBM2 Ml3XdWhVWyCjc4PhfS=> MWDxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= MlH6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
A673 NF;KcnlyUFSVIHHzd4F6 MYXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? NE\0cZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC M{i3OJFJXFNiYYPzZZk> NYHSPYdHeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> NGHueXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-37 M1LBVJFJXFNiYYPzZZk> NUPHN5h5eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB-EBc1 NHvUdFVyUFSVIHHzd4F6 MmTGdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= NUDpT5F3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
U-2 OS M2DnXJFJXFNiYYPzZZk> MmfxdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFVvMjDPV{Bk\Wyucx?= M{TRelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 M2Lje5FJXFNiYYPzZZk> M1;DepFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVYX;zMVIh[2WubIO= MkHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB1643 MVfxTHRUKGG|c3H5 NWKy[W9peUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsdy=> M4Du[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
LAN-5 M3Xub5FJXFNiYYPzZZk> MV\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVEGQLUWgZ4VtdHN? NXjoSm9{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-12 NH;XUIpyUFSVIHHzd4F6 M1:1VZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?= M{i5WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 NVn1dnZjeUiWUzDhd5NigQ>? NHf6NXFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnixPEBk\Wyucx?= M33CdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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NB-EBc1 MmrKdWhVWyCjc4PhfS=> MlLGdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIuTUKlMTDj[Yxtew>? M4S4[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 M1PSeZFJXFNiYYPzZZk> M2HobJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHNLNUeETUKgZ4VtdHN? M4LqTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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TC32 M4jTVJFJXFNiYYPzZZk> M{TQUZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> NE\jNGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) MorhdWhVWyCjc4PhfS=> MXfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDNS{A3OyBqNj3US{BTMSClZXzsdy=> MkHjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
U-2 OS MUTxTHRUKGG|c3H5 M4XLNJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHUuOiCRUzDj[Yxtew>? M2DJWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh41 NXy2U2I2eUiWUzDhd5NigQ>? NX\IdIJ6eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? NH35Wm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
RD NYL4b5ZZeUiWUzDhd5NigQ>? MnXHdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVmQh[2WubIO= NIq2VHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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Saos-2 NV;DU4NoeUiWUzDhd5NigQ>? NVPNV3BUeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy| NF\4WnY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
OHS-50 NYDF[ZJMeUiWUzDhd5NigQ>? M4PFVZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG9JWy13MDDj[Yxtew>? M2LZOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-SH M3XSU5FJXFNiYYPzZZk> MUTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNVOKIHPlcIx{ MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-S6K / S6K / p-AKT / AKT / IRS1 / p-4EBP1 / 4EBP1 / p-GSK3; 

PubMed: 23436801     


Torin2 is a potent mTOR inhibitor in HCT-116 cells

p-Chk1 / Chk1 / p-Chk2 / Chk2 / p-DNA-PK ; 

PubMed: 23436801     


Torin2 inhibits ATR (UV radiation), ATM and DNA-PK (ionizing radiation) strongly.

23436801
Immunofluorescence
LC3B ; 

PubMed: 23436801     


Hela cells were treated with indicated concentrations of Torin2 for 3 days stained with antibody specific for LC3B and DAPI. 

23436801
Growth inhibition assay
Cell viability; 

PubMed: 24434750     


Concentration–response curves of Torin 2, Torin 1 and WWH030 against gametocytes measured in the high throughout viability assay. Torin 1 was much weaker in potency compared to Torin 2.

24434750
体内研究

在小鼠肝微粒体稳定性试验中,Torin 2表现出>95%的药效学响应,和11.7分钟的半衰期。Torin 2静脉内或口服给药后,在雄性Swiss albino小鼠体内表现出最好的生物利用度(51%),短 的半衰期(0.72小时)和低清除率(19.6 mL/min/kg)。[1] 在Th-MYCN 小鼠体内,Torin 2(20mg/kg)消除MYCN肿瘤,减少MYCN蛋白质水平,并诱导细胞凋亡。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

- 合并

mTOR 和 PI3K 细胞试验:

对mTOR的细胞IC50值使用p53−/− MEFs测定。细胞用载体或逐渐增减浓度的Torin 2处理1小时,然后裂解。S6K1 Thr-389的磷酸化使用磷酸-特异性抗体进行免疫印迹监测。同时,对PI3Ka的细胞IC50值,在p53−/−/mLST8−/−MEFs或表达S473D突变体Akt1的人PC3细胞中,基于Akt Thr-308的磷酸化进行测定。
细胞实验:

[3]

- 合并
  • Cell lines: MZ-CRC-1 和 TT 细胞
  • Concentrations: 50 nM
  • Incubation Time: 3 天或 5 天
  • Method:

    对于活性测定,MZ-CRC-1和TT细胞以一式四份接种在96孔板(1.0×104细胞/孔),培养基分别包含2.5%和4% FBS。24小时后,细胞用Torin 2处理。在指定时间点,细胞与10 μL CellTiter96 AQueous One溶液在100 μL培养基中培育3小时,吸光度在490 nm下测量。


    (Only for Reference)
动物实验:

[1]

- 合并
  • Animal Models: 雄性 Swiss albino 小鼠
  • Dosages: 20 mg/kg
  • Administration: 静脉内或口服给药
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 20 mg/mL (46.25 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
1.5 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 432.4
化学式

C24H15F3N4O

CAS号 1223001-51-1
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

相关mTOR产品

Tags: 购买Torin 2 | Torin 2供应商 | 采购Torin 2 | Torin 2价格 | Torin 2生产 | 订购Torin 2 | Torin 2代理商
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID