Torin 2

目录号:S2817

仅限科研使用

Torin 2 是一种有效的选择性mTOR抑制剂,在p53−/− MEFs细胞系中IC50为0.25 nM;作用于mTOR比作用于PI3K选择性高800倍,并且改善了药代动力学性能。抑制ATM/ATR/DNA-PK,在PC3细胞系中EC50分别为28 nM/35 nM/118 nM。Torin 2 可降低细胞活力并诱导自噬与凋亡。

Torin 2 Chemical Structure

CAS: 1223001-51-1

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1562.81 现货
RMB 903.84 现货
RMB 3030.61 现货
大包装 有超大折扣
大剂量询单有大折扣!

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客户使用Selleck生产的Torin 2发表文献54篇:

产品安全说明书

mTOR抑制剂选择性比较

相关mTOR产品

生物活性

产品描述 Torin 2 是一种有效的选择性mTOR抑制剂,在p53−/− MEFs细胞系中IC50为0.25 nM;作用于mTOR比作用于PI3K选择性高800倍,并且改善了药代动力学性能。抑制ATM/ATR/DNA-PK,在PC3细胞系中EC50分别为28 nM/35 nM/118 nM。Torin 2 可降低细胞活力并诱导自噬与凋亡。
靶点
mTOR [1]
(p53−/− MEFs)
ATM [5]
(PC3 cells)
ATR [5]
(PC3 cells)
DNA-PK [5]
(PC3 cells)
0.25 nM 28 nM(EC50) 35 nM(EC50) 118 nM(EC50)
体外研究

Torin 2与PI3Kγ具有相同的结合模式,V882作为铰链结合点,在内部疏水袋Y867,D841 和D964再提供三个作用于氨基比林侧链的氢键,类似于mTOR的Y2225,D2195 和D2357。[1] Torin 2抑制mTORC1,因此通过促进其核转运激活TFEB,EC50为1.666 mM。[2] Torin 2(< 50 nM)引起MZ-CRC-1和TT细胞的活性显著减少。Torin 2 (100 nM)使MZ-CRC-1和TT细胞的迁移显著减少。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HCT116 NGXEXIZHfW6ldHnvckBie3OjeR?= MkP0NUBpeg>? NHnlb5FKdmirYnn0bY9vKG:oIH3UU3JEOSCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIGSzPFkheGixc4Doc5J6dGG2aX;uJI9vKFKSU{\LRlEh[W[2ZYKgNUBpeiCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZMtKEWFNUC9NE4xODB{Nd88US=> NF3zTXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUKxNVQ5OCd-MkmyNVE1QDB:L3G+
MOLM13 NHfyW2lCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MmjFRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPT1zNNVMh[2WubIOgZpkhS2WubD3UbZRmeiCJbH:gZZN{[XluIFnDOVA9OC5yMEJOwG0> MkWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB|N{C3OlYoRjNyM{ewO|Y3RC:jPh?=
MOLM14 NFjMcnBCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NGradY5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3PUG0yPCClZXzsd{BjgSCFZXzsMXRqfGW{IFfsc{Bie3OjeTygTWM2ODxyLkCwNe69VQ>? NGjlVVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEO3NFc3Pid-M{CzO|A4PjZ:L3G+
MV4-11 M4\EVGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NYrKeYtYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNWlQuOTFiY3XscJMh[nliQ3XscE1VcXSncjDHcI8h[XO|YYmsJGlEPTB:MD6wNFHPxE1? MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN5MEe2Okc,OzB|N{C3OlY9N2F-
HEK293T M3vpcmZ2dmO2aX;uJIF{e2G7 NEDF[YgzOCCvaX7z NVLGUWpFUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHscJkhTkyDRz30ZYdo\WRibWTPVmMyKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIFjFT|I6O1RiY3XscJMhfXOrbnegV|ZMOSCxcjDBb5QyKGG|IIP1ZpN1emG2ZTDh[pRmeiB{MDDtbY5{KGK7IHntcZVvd2Kub4T0bY5oKGG|c3H5MEBKSzVyPECuNFHPxE1? NV3WTmxRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmyNVE1QDBpPkK5NlEyPDhyPD;hQi=>
HeLa M2[0ZWZ2dmO2aX;uJIF{e2G7 NX\rSlN{OjBibXnudy=> NWi5epliUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHscJkhTkyDRz30ZYdo\WRibWTPVmMzKCi3bnvuc5dvKG:{aXfpckkh\XiycnXzd4VlKGmwIHj1cYFvKEinTHGgZ4VtdHNidYPpcochWz[NMTDvdkBCc3RzIHHzJJN2[nO2cnH0[UBi\nSncjCyNEBucW6|IHL5JIludXWwb3Lsc5R1cW6pIHHzd4F6NCCLQ{WwQFAvODIQvF2= NFmzVFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUKxNVQ5OCd-MkmyNVE1QDB:L3G+
RD MX;BcpRqfmm{YXygZZN{[Xl? M3HGPFQh\GG7cx?= MnXvRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgSY51\XKxdnnyeZMhSTdzIHnu[oVkfGWmIHnuJIh2dWGwIGLEJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC4aYL1d{1qdmS3Y3XkJINmdGxiZHXheIghcW6ldXLheIVlKG[xcjC0JIRigXNiYomgR4VtdFSrdHXyMWdtdyCudX3pcoV{[2WwY3WgZZN{[XluIFnDOVA:OC5yMd88US=> Mn3ZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzFyOEK3OlQoRjNzMEiyO|Y1RC:jPh?=
HCT116 M2W5eGZ2dmO2aX;uJIF{e2G7 MXexJIhz Ml;LTY5pcWKrdHnvckBw\iCDVF2gbY4hcHWvYX6gTGNVOTF4IHPlcIx{KGG|c3Xzd4VlKGG|IHHmeIVzKDFiaIKgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m|LDDFR|UxRTBwMEK4{txO NGjGfG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUKxNVQ5OCd-MkmyNVE1QDB:L3G+
HCT116 MX;GeY5kfGmxbjDhd5NigQ>? NIf1Z5oyKGi{ M{\v[mlvcGmkaYTpc44hd2ZiQWTSJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDh[pRmeiBzIHjyJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrczygSWM2OD1yLkCzOe69VQ>? MlL5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{MUG0PFAoRjJ7MkGxOFgxRC:jPh?=
RD NEixUHZEgXSxdH;4bYNqfHliYYPzZZk> NX\CW5ZvPCCmYYnz NHHwOHJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBTTCClZXzsd{BqdmO3YnH0[YQh\m:{IESg[IF6eyCkeTDD[YxtXGm2ZYKtS4xwKGy3bXnu[ZNk\W6lZTDhd5NigSxiQ1O1NF0xNjB2zszN NFXKU5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUC4Nlc3PCd-M{GwPFI4PjR:L3G+
HCT116 MYrGeY5kfGmxbjDhd5NigQ>? MY[xJIhz NX\W[2p4UW6qaXLpeIlwdiCxZjDEUmFRUyCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO|ZXSgZZMh[W[2ZYKgNUBpeiCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZMtKEWFNUC9NE4yOTkQvF2= NFvpd|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUKxNVQ5OCd-MkmyNVE1QDB:L3G+
PC3 NFW0VpBHfW6ldHnvckBie3OjeR?= M121W2lvcGmkaYTpc44hd2ZiUFmzT4FteGijIHnuJIh2dWGwIGDDN{Bk\WyuczDlfJBz\XO|aX7nJGFsfDFiU{S3N2QhdXW2YX70JIF{e2W|c3XkJIF{KHCqb4PwbI9zgWyjdHnvckBw\iCDa4SgWIhzOzB6IHL5JIludXWwb3Lsc5R1cW6pLDDFR|UxRTBwMt88US=> NGfXOmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUOyNlU3Pid-MkGzNlI2PjZ:L3G+
TC32 MmO4dWhVWyCjc4PhfS=> M1TBRpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCWQ{OyJINmdGy| MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
DAOY MUTxTHRUKGG|c3H5 M1n0O5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCGQV;ZJINmdGy| NYTr[YV6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SJ-GBM2 M3S3OJFJXFNiYYPzZZk> M2P5cJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz NE[5[2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
A673 NUDtOI5ZeUiWUzDhd5NigQ>? MoLkdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEF4N{OgZ4VtdHN? NUmwOXRzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-MC MVjxTHRUKGG|c3H5 NF\6fHVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=> M3HSflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 M1e0[ZFJXFNiYYPzZZk> MmHydWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUO3JINmdGy| MmHwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 NXHxTW96eUiWUzDhd5NigQ>? Mmi3dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= MmXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
U-2 OS M3X4WJFJXFNiYYPzZZk> MYTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhXS1{IF;TJINmdGy| M33XblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 M4TpdJFJXFNiYYPzZZk> NHnNOZZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU3Hvd{0zKGOnbHzz NXTqT3JDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB1643 M3XMdJFJXFNiYYPzZZk> NYTJVIVFeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsdy=> Mn\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 MoTudWhVWyCjc4PhfS=> M{GxT5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCOQV6tOUBk\Wyucx?= NI\6W2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 MXnxTHRUKGG|c3H5 NGHKOoFyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIO= MlqzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Rh18 MkXydWhVWyCjc4PhfS=> MWrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmhzODDj[Yxtew>? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
OHS-50 NGDw[5lyUFSVIHHzd4F6 MoC0dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= NG\jSYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
RD M1jmb5FJXFNiYYPzZZk> NW\icGpyeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLEJINmdGy| MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
MG 63 (6-TG R) NX\tdpJxeUiWUzDhd5NigQ>? NXuxZYFFeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF3HJFY{KCh4LWTHJHIqKGOnbHzz M363U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
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Assay
Methods Test Index PMID
Western blot p-S6K / S6K / p-AKT / AKT / IRS1 / p-4EBP1 / 4EBP1 / p-GSK3 p-Chk1 / Chk1 / p-Chk2 / Chk2 / p-DNA-PK 23436801
Immunofluorescence LC3B 23436801
Growth inhibition assay Cell viability 24434750
体内研究

在小鼠肝微粒体稳定性试验中,Torin 2表现出>95%的药效学响应,和11.7分钟的半衰期。Torin 2静脉内或口服给药后,在雄性Swiss albino小鼠体内表现出最好的生物利用度(51%),短 的半衰期(0.72小时)和低清除率(19.6 mL/min/kg)。[1] 在Th-MYCN 小鼠体内,Torin 2(20mg/kg)消除MYCN肿瘤,减少MYCN蛋白质水平,并诱导细胞凋亡。[4]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:

[1]

  • mTOR 和 PI3K 细胞试验:

    对mTOR的细胞IC50值使用p53−/− MEFs测定。细胞用载体或逐渐增减浓度的Torin 2处理1小时,然后裂解。S6K1 Thr-389的磷酸化使用磷酸-特异性抗体进行免疫印迹监测。同时,对PI3Ka的细胞IC50值,在p53−/−/mLST8−/−MEFs或表达S473D突变体Akt1的人PC3细胞中,基于Akt Thr-308的磷酸化进行测定。

细胞实验:

[3]

  • Cell lines: MZ-CRC-1 和 TT 细胞
  • Concentrations: 50 nM
  • Incubation Time: 3 天或 5 天
  • Method:

    对于活性测定,MZ-CRC-1和TT细胞以一式四份接种在96孔板(1.0×104细胞/孔),培养基分别包含2.5%和4% FBS。24小时后,细胞用Torin 2处理。在指定时间点,细胞与10 μL CellTiter96 AQueous One溶液在100 μL培养基中培育3小时,吸光度在490 nm下测量。

动物实验:

[1]

  • Animal Models: 雄性 Swiss albino 小鼠
  • Dosages: 20 mg/kg
  • Administration: 静脉内或口服给药

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O

1.5 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 432.4
化学式

C24H15F3N4O

CAS号 1223001-51-1
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

技术支持

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操作手册

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