Trichostatin A (TSA)

For research use only. Not for use in humans.

目录号：S1045 中文名称：曲古柳菌素A

CAS No. 58880-19-6

Trichostatin A (TSA)是一种HDAC抑制剂，无细胞试验中IC50为1.8 nM左右。

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2mg	RMB 2022.93	现货
5mg	RMB 5053.26	现货
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客户使用Selleck生产的Trichostatin A (TSA)发表文献196篇：

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产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述

Trichostatin A (TSA)是一种HDAC抑制剂，无细胞试验中IC50为1.8 nM左右。

靶点

HDAC ^[1]
(Cell-free assay)

~1.8 nM

体外研究

Trichostatin A 抑制八种乳腺癌细胞系，包括MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3增殖，平均IC50为124.4 nM (范围为26.4-308.1 nM),作用于表达ERα的细胞系比作用于表达 ERα阴性的细胞系更有效。Trichostatin A作用于全部乳腺癌细胞系，抑制HDAC活性，平均IC50为 2.4 nM (范围为0.6-2.6 nM), 产生显著的组蛋白 H4高度乙酰化。^[1]与 Trapoxin (TPX) 和 Chlamydocin 有效抑制HDAC1 或 HDAC4而不是 HDAC6不同，Trichostatin A 抑制这些HDACs，且抑制程度相似， IC50 分别为6 nM, 38 nM, 和8.6 nM。^[2] Trichostatin A (100 ng/mL) 作用于MIA PaCa-2细胞，通过招募p300和 PCAF进入Sp1-NF-Y HDAC复合体，而诱导转化生长因子βⅡ型受体(TβRII) 表达，复合体与TβRII 启动子的DNA片段结合，伴随着Sp1乙酰化，且与复合体相关的HDAC数理全部降低。^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
HeLa	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NH3kZlAyOC9\|MD:1NEBvVQ>?	NWew[YhqPzJiaB?=	NXvtUphJTE2VTx?=	MofsbY5kemWjc3XzJJRp\SCwdX3i[ZIhd2ZiTV3QJEjPnM7qbTmgcI9{eyClZXzsd{Bld3OnIHTldIVv\GWwdHz5	M1XPWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MU[1O\|Q5Lz5{M{G2OVc1QDxxYU6=
HeLa	NFzKVXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NXvDTWoxOjBibl2=	NWP6cWpLPzJiaB?=	NVT4SmJ1TE2VTx?=	Mm\rTWM2OCCxZjCyNI5O	NXvYPXJZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa	NHS1RpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4PjWVQxKG6P	NHHrXlY1QCCq	MXTEUXNQ	NEn0dGdKSzVyIH;mJFQxdk1?	NYOzOFF6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
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HCT116	MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVywMlIh\|ryP	M{f1bVEzKGh?		NV;vdVRk\W6qYX7j[ZMh[2WubDDhdI9xfG:\|aYOgbY5lfWOnZDDifUBz[WSrYYTpc44>	NIG1O\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGyNlI{OSd-MkSxNlIzOzF:L3G+
HLEB3	Ml;lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NUK0dodYOC52L{CuPEDPxE1?	MXe0PEBp	MV7EUXNQ	MUHpcoR2[2W\|IHPlcIwh[XCxcITvd4l{	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF3N{i3PEc,OjRzNUe4O\|g9N2F-
SRA01/04	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MknzNE41NzBwODFOwG0>	MX60PEBp	MVHEUXNQ	NYnqOYNWcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?=	M4eweVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUW3PFc5Lz5{NEG1O\|g4QDxxYU6=
HLEB3	MlLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MnTQNE4zKM7:TR?=	NF3jcW81QCCq	MUfEUXNQ	MV3zeZBxemW\|c3XzJINmdGxicILvcIln\XKjdHnvckB1cHKxdXfoJJN2eHC{ZYPzbY5oKHSqZTDQTVNMN0GtdDygdFM5VUGSSzDhcoQhTVKNMT:yJJNq\26jbHnu[{Bx[XSqd3H5dy=>	NGnGRYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1O\|g4QCd-MkSxOVc5Pzh:L3G+
SRA01/04	MkjWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3vBRVAvOiEQvF2=	NYK2eFFzPDhiaB?=	NXXUc4ZrTE2VTx?=	MkjVd5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hfGi{b4XnbEB{fXCycnXzd4lv\yC2aHWgVGk{Uy:Da4SsJJA{QE2DUFugZY5lKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfZM>	Mm\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNUe4O\|goRjJ2MUW3PFc5RC:jPh?=
A2780	MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M2P3TVAvODVvMjFOwG0>	Mn;QNVAwOjRxNEigbC=>	MnrVSG1UVw>?	M1vSfI1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{	NIXmTWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyN\|gxOSd-MkSyNlM5ODF:L3G+
SKOV3	MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mle4NE4xPS1{IN88US=>	NXfRT5NqOTBxMkSvOFghcA>?	NIrkdXBFVVOR	MkL3cYVlcWG2ZYOg[5Jwf3SqIHHydoV{fCCrbjDhJINwdmOnboTyZZRqd25vIHHu[EB1cW2nLXTldIVv\GWwdDDtZY5v\XJ?	NGfvcVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyN\|gxOSd-MkSyNlM5ODF:L3G+
Huh7	M1LWWmZ2dmO2aX;uJGF{e2G7	NYOwdZl[OC5zL{CuOU8yNjBizszN	M1OyTlI1cA>?		NGLhbYx{fXCycnXzd4V{KFmWSFTDNkBo\W6nIHX4dJJme3Orb36=	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ4OU[3Nkc,OjR{Nkm2O\|I9N2F-
hMSCs	NGHRcHFHfW6ldHnvckBCe3OjeR?=	MnKyOk4zPSCwTR?=	NFPLTo8zPCCq	MUfEUXNQ	MXPzeIFjcWyrenXzJGhqe3SxbnWgRYNmfHmuYYTpc44h[W6mIITo[UBGgHC{ZYPzbY9vKG:oIGDseZJqeG:2ZX70JGdmdmW\|	NUjnO2tsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzNVI{PTZpPkK0N\|EzOzV4PD;hQi=>
HT29	NVvWToJVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MXuxPFAhdk1?	MYG3NkBp		MmSzTWM2OCCxZjCxPFAhdk1?	NIDrfYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEO2PFI3PSd-MkSzOlgzPjV:L3G+
RPE	M3TPfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NXTiZmh2OC5{L{CuOE8xNjhxMTFOwG0>	NEXi[oMzPC92OD:3NkBp	M1HBfWROW09?	M3jocIlvcGmkaYTzJJRp\SCycn;sbYZmemG2aX;uJIJ6KGOnbHygZ5lkdGViYYLy[ZN1	M4HaTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NEW2OlAzLz5{NES1OlYxOjxxYU6=
U87 GBM	MXrGeY5kfGmxbjDBd5NigQ>?	NGeySowyODBxNUCwJG5u	NELqN5c1QCCq	MXWxNFAmKGW2aHHuc4w>	MYnJcoR2[2W\|IGPlcoV{[2WwY3WtUIls\SCDbITldoF1cW:wczDpckBPfWOuZXHyJG1wenCqb3zv[5k>	NX34N5c4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OlQ5PDFpPkK0OFY1QDRzPD;hQi=>
U87 GBM	MmjHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MWqxNFAwOzByL{WwNEBvVQ>?	NHjNbnY4OiCq	MWixNFAmKGW2aHHuc4w>	MXjy[YR2[2W\|IH3lZY4h[2WubDDueY1j\XJiYomgN\|EtKDV2LDDhcoQhPTkEoDW=	M{i0RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NE[0PFQyLz5{NES2OFg1OTxxYU6=
ACP03	NFnjN\|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4myV\|I2OC9\|NUCvOVAxdk1?	Mkf2NlRp		MlnP[IVkemWjc3XzJINmdGxidnnhZoltcXS7IH;mJIFxeHKxeHntZZRmdHliN{FCpEUh[XRiMkWwxsBvVQ>?	NYTCeW4zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2Olg2PDdpPkK0OlY5PTR5PD;hQi=>
ACP02	NYW1VW9VT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NWLid\|hjOjVyL{O1NE82ODCwTR?=	NUnOSppOOjSq		M1vvRoRm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDv[kBieHC{b4jpcYF1\Wy7IEewxsAmKGG2IEK1NOKhdk1?	NHKzUIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NE[2PFU1Pyd-MkS2Olg2PDd:L3G+
HeLa	MXTGeY5kfGmxbjDBd5NigQ>?	NFnHflEydU1?	MVGwMlVp		NVPiOIlz[WKxbHnzbIV{KHSqZTD0c5RidCCKRFHDJIFkfGm4aYT5	M1T3UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{C3OFc1Lz5{NEewO\|Q4PDxxYU6=
HMEC-1	NISybIpHfW6ldHnvckBCe3OjeR?=	NVzv[pRNOzBybl2=	MnPtNlRp		NV;CWVZGcW6lcnXhd4V{KGW6cILld5Nqd25ib3dCpHZGT0[UM9MgcXJPSQ>?	Ml\tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MUC2N\|EoRjJ2N{GwOlMyRC:jPh?=
HeLa	NIWyV2JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MnTSNu69VQ>?	M33jXFQ5cA>?		NEXPcmxl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZYJwfXRiMkWl	M176XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OES2NVM2Lz5{NEi0OlE{PTxxYU6=
LNCaP	NE\WZWdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MmnKNVAxNTFyMEDuUS=>	Mm\MNlRp		NHK4PWpKSzVyIH;mJFMxOG6P	M{L6W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEW0OlU5Lz5{NEi1OFY2QDxxYU6=
PC3	M4DFSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M2H0dVExOC1zMECwcm0>	NWrKSVl5OjSq		NWW2SYVMUUN3MDDv[kA{ODCwTR?=	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh3NE[1PEc,OjR6NUS2OVg9N2F-
CNE2	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4XKRlExOC14MEDuUS=>	NVHJXlNjOjRxNEivO\|Jp		M2GxTIlvcGmkaYTzJJRp\SCycn;sbYZmemG2aX;uJIlvKGFidHnt[U0h[W6mIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=>	NH7zV2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2PVkxOSd-MkS5Olk6ODF:L3G+
HeLa	M{XCc2Z2dmO2aX;uJGF{e2G7	MmT1NlUxdk1?	M1K0U\|E3cA>?	M2OybmROW09?	NXPlZ2FvcW6lcnXhd4V{KEO\UEHBNUBuWk6DIHX4dJJme3Orb39CpC=>	NF[xeXY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGxOlY5QCd-MkWxNVY3QDh:L3G+
A549	NWe3WpQ{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NU\ndJZROC5yMT2wMlnPxE1?	NEXtN5U4Omh?	MYPEUXNQ	MXrJR\|UxKG:oIECuNFbPxE1?	MoDpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkm0PVEoRjJ3MU[5OFkyRC:jPh?=
SKOV-3	NXvmRVFRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NV6xWmhKOC5yMT2wMlnPxE1?	M2nH[FczcA>?	NET1V5VFVVOR	NUXsfo83UUN3MDDv[kAxNjN{zszN	MljSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkm0PVEoRjJ3MU[5OFkyRC:jPh?=
A549	MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXuwMlEuOc7:TR?=	MnTuOFhp	MnfMSG1UVw>?	M17ZZmlEPTBib3[gNE4zQM7:TR?=	NXr0NYhvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOlk1QTFpPkK1NVY6PDlzPD;hQi=>
SKOV-3	MojyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MVqwMlEuOc7:TR?=	NYHBOnZpPDiq	MmXhSG1UVw>?	NXPoSZZpUUN3MDDv[kAxNjgQvF2=	NHfyXo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2PVQ6OSd-MkWxOlk1QTF:L3G+
A549	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mki1NU0yOM7:TR?=	NH7iR3MzPGh?	NVL0OWh{TE2VTx?=	MnTnTWM2OCCxZjCzMlLPxE1?	NVnJbJJrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOlk1QTFpPkK1NVY6PDlzPD;hQi=>
SKOV-3	M1fK[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M1HUdVEuOTEQvF2=	M174U\|I1cA>?	NHTONG1FVVOR	NFn4VG5KSzVyIH;mJFUvPs7:TR?=	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
MCF7	M4HTVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NXGx[5d6OC14MEDuUS=>	M1XtTVI1cA>?	NVfS[XdETE2VTx?=	MUTJR\|UxKG:oIEe1cm0>	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF7MkeyNUc,OjVzOUK3NlE9N2F-
MDA-MB-231	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4HodlAuPjBybl2=	M3HCOFI1cA>?	MV3EUXNQ	MWDJR\|UxKG:oIEGwNI5O	NEHIS\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG5NlczOSd-MkWxPVI4OjF:L3G+
A431	M2[xc2Z2dmO2aX;uJGF{e2G7	M3nCXFUxdk1?	NIPoS2EzNzZxMUKvNlRp	NYWz[ZYyTE2VTx?=	NXHkUYJr[WO2aY\heIV{KHB{MTDhcoQhcW6qaXLpeJMhSVSIMzDlfJBz\XO\|aX;u	NHnhT3U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO3NVA3QSd-MkWzO\|ExPjl:L3G+
A431	MXfBdI9xfG:\|aYOgRZN{[Xl?	M1fQN\|IwOTBxNUCvNVAxdk1?	MlLjOFhp	M4TtO2ROW09?	NXu2[HlxcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4To	NEnVbmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO3NVA3QSd-MkWzO\|ExPjl:L3G+
PC3	M1XNbGZ2dmO2aX;uJGF{e2G7	NWXXO4NDOC5zzszN	M{nIV\|Q5cA>?	M4PmXmROW09?	MYTpcoR2[2W\|IHnuZ5Jm[XOnIH;mJGhFSUNzIHHu[EBJTEGFMjDvckBUdHWpIHflcoV{KHC{b33veIVz	M2T3SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
SW480	M3HnSWZ2dmO2aX;uJGF{e2G7	MlrTNE4y\|ryP	M3n6RVQ5cA>?	NGnl[ZBFVVOR	NW\OfY1QcW6mdXPld{BqdmO{ZXHz[UBw\iCKRFHDNUBidmRiSFTBR\|Ihd25iU3z1[{Bo\W6nczDwdo9ud3Snch?=	NFf2e4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzOFk6Pyd-MkW0N\|Q6QTd:L3G+
PC3	NG\nb49HfW6ldHnvckBCe3OjeR?=	NY[2OHlHOC5zzszN	M4SyfVQ5cA>?	NWexNJJHTE2VTx?=	MnrxZZR1\W63YYTld{Bqdn[jc3nvckBidmRibXnndoF1cW:w	NWrrUlBqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N\|Q6QTdpPkK1OFM1QTl5PD;hQi=>
PC3	NUjP[pB7TnWwY4Tpc44hSXO\|YYm=	M3XhdFAvOc7:TR?=	MnHYOFhp	M{[yXWROW09?	NV3TTVZOemW4ZYLz[ZMhTU2W	NVjGVoR5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N\|Q6QTdpPkK1OFM1QTl5PD;hQi=>
SW480	MYLGeY5kfGmxbjDBd5NigQ>?	MX[wMlHPxE1?	NY\YRXlSPDiq	MoXqSG1UVw>?	NHLIO\|hifHSnboXheIV{KGmwdnHzbY9vKGGwZDDtbYdz[XSrb36=	NVezSnh3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N\|Q6QTdpPkK1OFM1QTl5PD;hQi=>
SW480	NH3EXXRHfW6ldHnvckBCe3OjeR?=	M163ZVAvOc7:TR?=	NEXxXWI1QGh?	NF7LeWRFVVOR	M4fSSZJmfmW{c3XzJGVOXA>?	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR\|NEm5O{c,OjV2M{S5PVc9N2F-
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melanoma B16/BL6	MX3GeY5kfGmxbjDhd5NigQ>?				NF;CWnFKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcGm\|dH;u[UBl\WGlZYT5cIF{\XNiKFjERWMyMSCycnXwZZJm\CCocn;tJI1wfXOnIH3lcIFvd22jIFKxOk9DVDZiY3XscJMtKEmFNUCgQUAxNjBzOTFOwG0v	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
melanoma B16/BL6	MWnGeY5kfGmxbjDhd5NigQ>?				MUfJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbIl{fG:wZTDk[YFk\XS7bHHz[UApUESDQ{SpJJBz\XCjcnXkJIZzd21ibX;1d4UhdWWuYX7vcYEhSjF4L1LMOkBk\WyuczygTWM2OCB;IECuNFIh\|ryPLh?=	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
melanoma B16/BL6	MorJSpVv[3Srb36gZZN{[Xl?				MVnJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbIl{fG:wZTDk[YFk\XS7bHHz[UA3KHC{ZYDhdoVlKG[{b32gcY92e2VibXXsZY5wdWFiQkG2M2JNPiClZXzsd{whUUN3MDC9JFAvODJ6IN88UU4>	NHLvOWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUGwPVYzPid-MUWxNFk3OjZ:L3G+
melanoma B16/BL6	M1LsZWZ2dmO2aX;uJIF{e2G7				NHLNdXpKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcGm\|dH;u[UBl\WGlZYT5cIF{\SB6IIDy[ZBiemWmIH\yc40hdW:3c3WgcYVt[W6xbXGgRlE3N0KONjDj[YxteyxiSVO1NEA:KDBwMESg{txONg>?	NFPkV2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUGwPVYzPid-MUWxNFk3OjZ:L3G+
293T	MlvZSpVv[3Srb36gZZN{[Xl?				MmjqTY5pcWKrdHnvckBw\iCqdX3hckBpcXO2b37lJIRm[WOndInsZZNmKDRicILldIFz\WRiZoLvcUAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yMjFOwG0v	MonFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyNlQoRjF3NEW0NlI1RC:jPh?=
293T	MV\GeY5kfGmxbjDhd5NigQ>?				Mn3ITY5pcWKrdHnvckBw\iCqdX3hckBpcXO2b37lJIRm[WOndInsZZNmKDFicILldIFz\WRiZoLvcUAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yMjFOwG0v	MkT1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyNlQoRjF3NEW0NlI1RC:jPh?=
293T	NUnsfHRpTnWwY4Tpc44h[XO\|YYm=				MVTJcohq[mm2aX;uJI9nKGi3bXHuJIhqe3SxbnWg[IVi[2W2eXzhd4UhPiCycnXwZZJm\CCocn;tJFI6O1RiY3XscJMtKEmFNUCgQUAxNjB{IN88UU4>	Mnv3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyNlQoRjF3NEW0NlI1RC:jPh?=
293T	NGe3N\|VHfW6ldHnvckBie3OjeR?=				MnPNTY5pcWKrdHnvckBw\iCqdX3hckBpcXO2b37lJIRm[WOndInsZZNmKDhicILldIFz\WRiZoLvcUAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yNDFOwG0v	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR3NEKyOEc,OTV2NUSyNlQ9N2F-
HeLa	NUnMOpFpTnWwY4Tpc44h[XO\|YYm=				NXrlemN4UW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgbJVu[W5iaHnzeI9v\SCmZXHj[ZR6dGG\|ZTCoTGRCSyliZoLvcUBJ\UyjIHPlcIx{KHerdHigd5Vje3S{YYTlJFViNCCLQ{WwJF0hOC5yNUS1JO69VS5?	NIDCcYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUS1OlI3Pyd-MUW0OVYzPjd:L3G+
HCT116	MY\BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?				NYG3dJZYSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBJS1RzMU[gZ4VtdHNuIFnDOVAhRSByLkCwNVMh\|ryPLh?=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjlyNEi5NEc,OTZ7MES4PVA9N2F-
H1299	NEL5VmFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				Ml7GRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDINVI6QSClZXzsd{whUUN3MDC9JFAvOSEQvF2u	M3jNNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUC0PFkxLz5zNkmwOFg6ODxxYU6=
H661	M3XiVmFvfGmycn;sbYZmemG2aY\lJIF{e2G7				MmH6RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDIOlYyKGOnbHzzMEBKSzVyIE2gNE4{KM7:TT6=	NGK0WWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmwOFg6OCd-MU[5NFQ5QTB:L3G+
H661	MmLxRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		Mn7DOFghcHK\|		M3q1c2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgTFY3OSClZXzsd{Bi\nSncjC0PEBpenNiYomgXHRVKGG\|c3H5MEBKSzVyIE2gNE4xQDVizszNMi=>	NVvQZ5BtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NlQ4PzNpPkG3OlI1Pzd\|PD;hQi=>
WiDr	Mn;mS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?				NEGzb2ZKdmirYnn0bY9vKG:oIGPBVFE{OCCvZXTpZZRm\CClZXzsJIdzd3e2aDDpckBpfW2jbjDXbWRzKGOnbHzzMEBKSzVyIE2gNE42Pzd4IN88UU4>	NYLnXW5CRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OFMyOTJpPkG3OlQ{OTF{PD;hQi=>
U251	NWKxNIF7TnWwY4Tpc44h[XO\|YYm=				MXLJcohq[mm2aX;uJI9nKFODUEGzNEBqdiCYRVfGMZN1cW23bHH0[YQhcHWvYX6gWVI2OSClZXzsd{BjgSCSTFHQJJJmeG:{dHXyJIdmdmViYYPzZZktKEmFNUCgQUAxNjZzMUKg{txONg>?	NFnnV\|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[0N\|EyOid-MUe2OFMyOTJ:L3G+
293T	NEf2[5NHfW6ldHnvckBie3OjeR?=				MXvJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNzIHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yMEG5JO69VS5?	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh6MUKzNkc,OTd6OEGyN\|I9N2F-
293T	Mn\USpVv[3Srb36gZZN{[Xl?				MULJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN2IHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yMEKg{txONg>?	M{LlelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEixNlMzLz5zN{i4NVI{OjxxYU6=
293T	M3riT2Z2dmO2aX;uJIF{e2G7				NF7vOGZKdmirYnn0bY9vKG:oIH3veZNmKEiGQVO2JIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6wNFI5KM7:TT6=	NH:1NVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i4NVI{Oid-MUe4PFEzOzJ:L3G+
H661	NUXiUoxHSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		M{DHZVQ5KGi{cx?=		MX3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFi2OlEh[2WubIOgZYZ1\XJiNEigbJJ{NCCLQ{WwJF0hOC5yOEWg{txONg>?	MlPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OUe4NlQoRjF5OEm3PFI1RC:jPh?=
293T	NIXWTlhHfW6ldHnvckBie3OjeR?=				MYLJcohq[mm2aX;uJI9nKEiGQVOxJIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6xPUDPxE1w	NWL4dWhwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
293T	MnTYSpVv[3Srb36gZZN{[Xl?				M2PWTGlvcGmkaYTpc44hd2ZiSFTBR\|Qh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOsJGlEPTBiPTCwMlIh\|ryPLh?=	MnizQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7MEC5NVEoRjF5OUCwPVEyRC:jPh?=
293T	M2GwZmZ2dmO2aX;uJIF{e2G7				MoPZTY5pcWKrdHnvckBw\iCKRFHDOkBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNlgh\|ryPLh?=	MkXpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7MEC5NVEoRjF5OUCwPVEyRC:jPh?=
NIH3T3	M2fIbmZ2dmO2aX;uJIF{e2G7				NVjQUGVuUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOSCneIDy[ZN{\WRiaX6gUmlJO1R\|IHPlcIx{NCCLQ{WwJF0hOC5yMkGg{txONg>?	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl{OUe5PEc,OTd7Mkm3PVg9N2F-
human 293	M{\aTmZ2dmO2aX;uJIF{e2G7				MVvJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO0JIV5eHKnc4Pl[EBqdiB{OUOgZ4VtdHNuIFnDOVAhRSByLkCzOEDPxE1w	NEHJVJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{myPVc6QCd-MUe5Nlk4QTh:L3G+
mouse 293	NILXNGVHfW6ldHnvckBie3OjeR?=				M36wfmlvcGmkaYTpc44hd2ZibX;1d4UhemWlb33ibY5idnRiSFTBR\|Yh\XiycnXzd4VlKGmwIEK5N{Bk\WyuczygTWM2OCB;IECuNFgyKM7:TT6=	MkHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7Mkm3PVgoRjF5OUK5O\|k5RC:jPh?=
SKBR3	NVniS4JjSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				NYH1[WNvSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT2JTOyClZXzsd{whUUN3MDC9JFAvODJizszNMi=>	NWH4UHpkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
MM96L	MUHGeY5kfGmxbjDhd5NigQ>?				NF\kb3RKdmirYnn0bY9vKG:oIHj1cYFvKE2POU\MJINmdGy\|LDDJR\|UxKD1iMD6wN{DPxE1w	NUHPeHNjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
SNU16	Mor4SpVv[3Srb36gZZN{[Xl?				MUjJcohq[mm2aX;uJI9nKEiGQVOg[pJwdSCqdX3hckBUVlVzNjDj[YxteyxiSVO1NEA:KDBwMEWzJO69VS5?	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
A549	NXPPOYVwSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				NVjxVXAxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzMEBKSzVyIE2gNE4xQCEQvF2u	M3nNZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
MKN45	M4W4bGFvfGmycn;sbYZmemG2aY\lJIF{e2G7				MWTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2NTkS1JINmdGy\|LDDJR\|UxKD1iMD6xJO69VS5?	MoHHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEe1OVQoRjF6MkS3OVU1RC:jPh?=
SQ20B	MYfGeY5kfGmxbjDhd5NigQ>?				NGC4R2JKdmirYnn0bY9vKG:oIHj1cYFvKFOTMkDCJINmdGy\|LDDJR\|UxKD1iMD6yJO69VS5?	NVLnVmZORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
neonatal foreskin fibroblasts	NUH5VYxjT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=				NVPROHpTT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hdmWxbnH0ZYwh\m:{ZYPrbY4h\mmkcn;icIF{fHNiY3XscJMtKEmFNUCgQUAxNjJizszNMi=>	M1HCR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
HeLa	NEG0XJBHfW6ldHnvckBie3OjeR?=				M2DkWmlvcGmkaYTpc44hd2ZiSFTBR{Bnem:vIHj1cYFvKEinTHGgZ4VtdHNuIFnDOVAhRSByLkOg{txONg>?	NWOyUVZ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
MM96L/NFF	NHr5XYZHfW6ldHnvckBie3OjeR?=				MYrT[Yxm[3Srdnn0fUBqdmSneDygdoF1cW9ib3[gTWM2OCCob4KgbJVu[W5iTV25Omwh[2WubIOgeI8hUUN3MDDmc5IhcHWvYX6gUmZHKGOnbHzzMEBKSzVyIE2gOk44KM7:TT6=	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
293T	M2TvNmZ2dmO2aX;uJIF{e2G7				MV;Jcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNzIHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yM{[2JO69VS5?	NGjXXpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5O\|gzPid-MUizPVc5OjZ:L3G+
293T	NYL5[5NCTnWwY4Tpc44h[XO\|YYm=				MlfpTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFNDDlfJBz\XO\|ZXSgbY4hOjl\|VDDj[YxteyxiSVO1NEA:KDBwME[yPUDPxE1w	NFHVVXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5O\|gzPid-MUizPVc5OjZ:L3G+
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insect cell	MV\GeY5kfGmxbjDhd5NigQ>?		M1u4[VYxKG2rboO=		NVKweldnUW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBz\WOxbXLpcoFvfCCqdX3hckBENXSncn3pcoFtKEircz30ZYdo\WRiSFTBR\|MwVi22ZYLtbY5idCCJU2SteIFo\2WmIILlZ49u[mmwYX70JIh2dWGwIF7DU3IzKCh\|OUWgeI8hPDh7IILld4llfWW\|KTDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCjZoTldkA3OCCvaX7zJIJ6KG[udX;ybY1mfGW{LDDLbUA:KDBwMECwPFQh\|ryPLh?=	MkfKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
insect cell	NXP0XJVITnWwY4Tpc44h[XO\|YYm=		NEKwUo43OCCvaX7z		M4HhfmlvcGmkaYTpc44hd2ZiZoXscEBt\W6pdHigdoVkd22kaX7hcpQhcHWvYX6gR{11\XKvaX7hcEBIW1RvdHHn[4VlKEiGQVOyJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3cz3pcoZm[3SnZDDpcpNm[3RiY3XscJMhfXOrbnegVmhMUyiDYznBUWMh[XNic4Xid5Rz[XSnIHHmeIVzKDZyIH3pcpMh[nliZnz1c5JqdWW2ZYKsJGtqKD1iMD6wNFM2PSEQvF2u	NHPmO3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
insect cell	MWnGeY5kfGmxbjDhd5NigQ>?				NYTLeYRbUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDISGFEOTBiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{NWmwZnXjeIVlKGmwc3XjeEBk\WyuczD1d4lv\yCobIXvdo9o\W6rYzDw[ZB1cWSnIGLIT2tC[yCjczDzeYJ{fHKjdHWgZpkh\my3b4LpcYV1\XJuIFvpJF0hOC5yMES3OkDPxE1w	NWPB[VRPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
insect cell	M4\WTGZ2dmO2aX;uJIF{e2G7		MlHaOlAhdWmwcx?=		MlrUTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCFLYTldo1qdmGuIFjpd{11[WepZXSgTGRCSzhiKEGgeI8hOzd5IILld4llfWW\|KTDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT{hC[ymNKFHjLWFOSyCjczDzeYJ{fHKjdHWgZYZ1\XJiNkCgcYlveyCkeTDmcJVwemmvZYTldkwhU2liPTCwMlE1PSEQvF2u	NVniblVYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
insect cell	MVXGeY5kfGmxbjDhd5NigQ>?				MYrJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKE5vdHXycYlv[WxiSHnzMZRi\2enZDDISGFEOTFiKEGgeI8hOzR5IILld4llfWW\|KTDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCkeTDmcJVwemmvZYTldkwhU2liPTCwMlQxOSEQvF2u	MnPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
GM15850	NWizRYl5TnWwY4Tpc44h[XO\|YYm=	M2OzcVAvOSC3TR?=	MofLNVIhcHK\|		MXjJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gS20yPTh3MDDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckB1d3SjbDDhZ4V1gWyjdHXkJIhqe3SxbnWgcIV3\WxiYYSgNE4yKHWPIHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
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ST14A	MlG2SpVv[3Srb36gZZN{[Xl?	NI\hcFQ4PSCwTR?=			Mki1TY5lfWO2aX;uJI9nKFKHU2SvUnJUTi2lb4XwcIVlKHKncILld5Nqd25ib3[gcJVkcW[ncnHz[UB1[WepZXSgdoF1KFKHMT;OVnNGKEKGTl[gdJJwdW:2ZYKgZYN1cX[rdImg[ZhxemW\|c3XkJIlvKFOWMUTBJINmdGy\|IHH0JFc2KG6PIHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGy3Y3nm[ZJie2ViYXP0bZZqfHl?	M3TGS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NU[1PVk{Lz5zN{W2OVk6OzxxYU6=
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HEK293	M2XGU2Z2dmO2aX;uJIF{e2G7	NUnOSmZuPjByIH7N			MlntTY5kemWjc3WgbY4heHKxdHXpckBlcXO3bH\p[IUhcXOxbXXyZZNmKGyndnXsJIlvKEiHS{K5N{Bk\WyuczDheEA3ODBibl2gZpkhcW2vdX7vZoxwfA>?	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV4NkezNkc,OTd3Nk[3N\|I9N2F-
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HCT116	NUXSToRiTnWwY4Tpc44h[XO\|YYm=				NFrkc3dKdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO2JIF{e2W\|c3XkJIF{KHS3YoXsbY4hcHmyZYLhZ4V1gWyjdHnvckBqdiCqdX3hckBJS1RzMU[gZ4VtdHNiYomgW4V{fGW{bjDicI91	Mo\VQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNUSyN\|koRjF6MEW0NlM6RC:jPh?=
HEK293 STF	MkDXSpVv[3Srb36gZZN{[Xl?		M2C3c\|I1KGi{cx?=		MW\BZ5RqfmG2aX;uJI9nKG2xdYPlJHdvfDOjIIPp[45idGmwZzDlfJBz\XO\|ZXSgbY4hUEWNMkmzJHNVTiClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHliYX\0[ZIhOjRiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[U1j[XOnZDDsZYN1[XSnIHTlbJllem:pZX7hd4Uh[XO\|YYm=	MkHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyMkK2O\|UoRjF7MEKyOlc2RC:jPh?=
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HeLa	M{GyfGZ2dmO2aX;uJIF{e2G7	NXjwUnI4OSC3TR?=	M3nGU\|ExKG2rboO=		NYX5eIdSUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHGgZ4VtdHNiYYSgNUB2VSCjZoTldkAyOCCvaX7zJIJ6KG[udX;yc41mfHKrYzDhd5NigQ>?	NVfWVWd4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwOVU5PDhpPkKxNFU2QDR6PD;hQi=>
MCF7	MV7GeY5kfGmxbjDhd5NigQ>?	NVXTOHZ{OC53IIXN	MXqyOEBpenN?		MXvJcohq[mm2aX;uJI9nKEOuYYPzJFEhcGm\|dH;u[UBl\WGlZYT5cIF{\SCrbjDoeY1idiCPQ1[3JINmdGy\|IHHzd4V{e2WmIHHzJJVxNXKnZ4XsZZRqd25ib3[gdFIyKHC{b4TlbY4hdGW4ZXygZZQhOC53IIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM>	MmSxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OECzOlMoRjJ{MkiwN\|Y{RC:jPh?=
MCF7	NGexS2dHfW6ldHnvckBie3OjeR?=	MUiwMlUhfU1?	NX\p[I9iOjRiaILz		M2jSPWlvcGmkaYTpc44hd2ZiSFTBR\|YhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDjbIFv\2ViaX6geJVjfWyrbjDhZ4V1gWyjdHnvckBt\X[nbDDheEAxNjVidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	M4jpZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkiwN\|Y{Lz5{MkK4NFM3OzxxYU6=
MCF7	NGTSWIFHfW6ldHnvckBie3OjeR?=	M4P4bVAvPSC3TR?=	NUDUcG5FOjRiaILz		NGrFRW9KdmirYnn0bY9vKG:oIFjERWMyKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgeZAuemWpdXzheIlwdiCxZjDhZ4V1gWyjdHXkJIhqe3SxbnWgTFMheHKxdHXpckBt\X[nbDDheEAxNjVidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	NVflcIZmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyPFA{PjNpPkKyNlgxOzZ\|PD;hQi=>
PC12	NV\LdZZQTnWwY4Tpc44h[XO\|YYm=	MmrzNVAhfU1?	MnrUOFghcHK\|		MonTTY5pcWKrdHnvckBw\iCKRFHDOkBqdiC{YYSgVGMyOiClZXzsd{Bie3Onc4Pl[EBieyCjY3X0fYxifGmxbjDv[kBpcXO2b37lJGg{KGG2IEGwJJVOKGGodHXyJFQ5KGi{czDifUBY\XO2ZYLuJIJtd3S2aX7nJIFv[Wy7c3nz	M1z1[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
PC12	NGXxRVhHfW6ldHnvckBie3OjeR?=	Mnu3NVAhfU1?	NIXYPY01QCCqcoO=		NFLRZ5FKdmirYnn0bY9vKG:oIFjERWM3KGmwIILheEBRSzF{IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBidHCqYT30eYJ2dGmwIHHj[ZR6dGG2aX;uJIF1KDFyIIXNJIFnfGW{IES4JIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6pIHHuZYx6e2m\|	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyNU[4NEc,OjN7MEW2PFA9N2F-
PC12	NEW4U2VHfW6ldHnvckBie3OjeR?=	NVviZ2xCOTBidV2=	M{K1TlUh\GG7cx?=		M3r4dGlv\HWldHnvckBw\iCwZYXybZRmKG:3dHfyc5d1cCCrbjDyZZQhWENzMjDj[YxteyCjdDCxNEB2VSCjZoTldkA2KGSjeYO=	NV\mR3cyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
SH-SY5Y	MorFSpVv[3Srb36gZZN{[Xl?	M1vnWlExKHWP	NVXEbJlKPSCmYYnz		NHHIR\|NKdmS3Y4Tpc44hd2ZibnX1dol1\SCxdYTndo94fGhiaX6gbJVu[W5iU1itV3k2YSClZXzsd{BifCBzMDD1UUBi\nSncjC1JIRigXN?	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyNU[4NEc,OjN7MEW2PFA9N2F-
A549	NUDpfGRESW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	NEXFdVYxNjRidV2=	NITZeGIzPCCqcoO=		MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgd4hzfW6tZX6gZY5lKHOqYYLwJJNp[XCnIHH0JFAvPCC3TTDh[pRmeiB{NDDodpMh[nlicHjhd4Uh[2:wdILhd5QhdWmlcn;zZ49xgQ>?	M3XVO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEi0NVEzLz5{NUi4OFEyOjxxYU6=
HeLa	MmTvSpVv[3Srb36gZZN{[Xl?				Mm\BTY5pcWKrdHnvckBw\iCKRFHDOkBqdiCqdX3hckBJ\UyjIHPlcIx{KGG\|c3Xzd4VlKGG\|IIT1ZpVtcW5iYXPleJlt[XSrb36gbY5kfWKjdHXkJIZweiCxdnXycolocHRiYomgbY1ufW6xZnz1c5Jme2OnbnPlJI1q[3Kxc3PvdIlkKGGwYXz5d4l{	NW\vUoRKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2NVE6OTlpPkK2OlEyQTF7PD;hQi=>
C127-LT	NEjVW\|JHfW6ldHnvckBie3OjeR?=	NWToR3NxOjByIH7N	NUX1fHVSOjRiaILz		MX3Jcohq[mm2aX;uJI9nKEiGQVOgbY4hdW:3c3WgR\|EzPy2OVDDj[YxteyCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKEWJRmCg[ZhxemW\|c3nvckBifCB{MECgcm0h[W[2ZYKgNlQhcHK\|IHL5JIZtfW:{ZYPj[Y5k\SCvaXPyc5Nkd3C7	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl7NkO3Nkc,OjZ7OU[zO\|I9N2F-
HeLa nuclear	M4XsO2Z2dmO2aX;uJIF{e2G7	MVmxNEB1dyB{NTD1US=>	MXmzNEBucW6\|		MVTDc41x\XSrdHn2[UBqdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYTDueYNt\WG{IHPlcIx{KGG2IEGwJJRwKDJ3IIXNJJV{cW6pIFLvZ{1i[2W2eXytcJl{cW6nLVHNR{BieyC\|dXLzeJJifGViYX\0[ZIhOzBibXnud{BjgSCOaX7le4VifmW{LVL1dosheGyxdDDhcoFtgXOrcx?=	MnvTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7OU[zO\|IoRjJ4OUm2N\|czRC:jPh?=
HeLa	NW\VbZRMTnWwY4Tpc44h[XO\|YYm=	M2jxNFIhfU1?	M3\1flI1KGi{cx?=		MnG5TY5pcWKrdHnvckBw\iCKRFHDNUBqdiCqdX3hckBJ\UyjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHj5dIVz[WOndInsZZRqd25ib3[gbIl{fG:wZTDIN{BifCB{IIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	NXv1OIcyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewOlA4PjRpPkK3NFYxPzZ2PD;hQi=>
HN13	MUfGeY5kfGmxbjDhd5NigQ>?		MoXqNlQhcHK\|		MUnJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTG4yOyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gbIl{fG:wZTDIN{BNgXN7IHHj[ZR6dGG2aX;uJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHntcZVvd2[udX;y[ZNk\W6lZTDhd5NigQ>?	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB\|MUC2Okc,OjlyM{GwOlY9N2F-
HeLaS3	NFvvUZlHfW6ldHnvckBie3OjeR?=	MkPONVAhfU1?			NUTIdlVsUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHHTN{Bk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCqaYP0c45mKEh\|S{mgZYNmfHmuYYTpc44h[XRiMUCgeW0hdWWjc4Xy[YQhcW2vZXTpZZRmdHliYomgbY1ufW6xYnzveEBidmGueYPpdy=>	NEn2dY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEK1PFU{PSd-M{CyOVg2OzV:L3G+
HeLaS3	M3LPWGZ2dmO2aX;uJIF{e2G7	M2LQZ\|ExKHWP	NEi3cnIyPiCqcoO=		NXnMWWFmUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHHTN{Bk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCqaYP0c45mKEh\|S{mgZYNmfHmuYYTpc44h[XRiMUCgeW0hfHKnYYTl[EBnd3JiMU[gbJJ{KG[xbHzve4VlKGK7IHPvcZBwfW6mIIfhd4hwfXRiYX7kJI1m[XO3cnXkJJVxKHSxIEK0JIhzeyCkeTDpcY12dm:kbH;0JIFv[Wy7c3nz	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N\|U9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	EGFR / STAT3 ; PubMed: 31289542 Oncol Lett Expression of EGFR and STAT3 in PC3 cells treated with TSA. PC3 cells were plated in 6-cm dishes, and 0.25, 0.5 or 1 µM TSA was added for 24 h. DMSO was added into the control group wells. Cells were collected and lysed with lysis buffer and the protein expression analyzed via western blotting. Cyclin D1 / CDK6 / CDK4 / p-RB / RB; PubMed: 31289542 Oncol Lett Expression of cyclin D1, CDK6, CDK4 and RB, and the phosphorylation of RB, was detected in PC3 cells treated with TSA for 24 h. MMP-1 / MMP-3 / MMP-13 / TIMP-1; PubMed: 27431944 Mol Med Rep Chondrocytes were pretreated with increasing concentrations of TSA for 2 h, followed by stimulation with 5 ng/ml IL-1β for 24 h. Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1); PubMed: 27431944 Mol Med Rep Chondrocytes were treated with increasing concentrations of TSA for 24 h. α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2; PubMed: 23802098 Front Oncol Histone acetylation occurs directly at the promoter region of IL-23R. The ChIP assay demonstrates that TSA treatment results in an increase in the acetylation of histone H3 and H4. A549 cells were cultured in the presence or absence of TSA (250 ng/mL) for a period of 24 h. Subsequently, a ChIP assay was performed using the following antibodies; pan acetyl-histone H3 (H3Ac), pan acetyl-histone H4 (H4Ac), acetyl-histone H3 Lys 9 (H3K9Ac), acetyl-histone H3 Lys 9/14 (H3K9/14ac), acetyl-histone H3 Lys 9 phosphoSer10 (H3K9S10), methyl-histone H3 Lys 4 (H3K4Me), di methyl-histone H3 Lys 4 (H3K4Me2), and di methyl-histone H3 Lys 9 (H3K9Me2). Input DNA serves as a positive control as recommended by the manufacturer (Diagenode). A no antibody control was included to test for non-specific binding (UT, untreated; TSA, Trichostatin A).	31289542 27431944 23802098
Immunofluorescence	α-C/EBPβ / α-HP1α ; PubMed: 21122806 Exp Cell Res 3T3-L1 preadipocytes were grown on coverslips for three days and induced to differentiate by treatment with MDI (48 h) in the presence of vehicle (-TSA) or 87 nM Trichostatin A (+TSA). Under these experimental conditions: A-H Cells were fixed and permeabilized in cold methanol, subjected to IIF with the indicated antibodies, and nuclei were counterstained with DAPI. Scale bar, 5 μm. α-HDAC1 ; PubMed: 29045501 PLoS One MCF-7 cells were treated with indicated concentrations of trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels. α-tubulin / acetylated histone 3; PubMed: 27957719 Neurotherapeutics Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775.	21122806 29045501 27957719
Growth inhibition assay	Cell viability (A549 cells); PubMed: 27571418 PLoS One Cytotoxicity of histamine determined by MTT assay in A549 cells. Cell viability (MDA-MB-231 cells); PubMed: 27548148 Int J Mol Sci The human breast cancer cells (MDA-MB-231) were incubated with various concentrations of TSA (0–300 nM) for 24 h. Cell viability was measured by WST-8. Cell viability (PC3 cells); PubMed: 31289542 Oncol Lett PC3 cells were plated in a 96-well plate and treated with different doses of TSA (0, 0.25, 0.5 and 1 µM) for 72 h; an MTT assay was used to measure the effects of TSA on PC3 cell proliferation.	27571418 27548148 31289542

体内研究

Administration of Trichostatin A 按 0.5 mg/kg 剂量处理N-甲基-N-亚硝基脲致癌物诱发的大鼠乳腺癌模型，持续处理4周，具有有效的抗癌活性，即使按按高达5 mg/kg剂量处理也没有任何可测量到的毒性。^[1]Trichostatin A 按10 mg/kg 剂量单独腹腔注射给药非转基因和脊髓性肌萎缩症（SMA）模型鼠，导致乙酰化的 H3 和 H4组蛋白水平提高，也导致活运动神经元(SMN) 基因表达稍微提高。Trichostatin A 每天按10 mg/kg剂量处理SMA模型鼠，促进存活, 减轻体重下降,且增强运动行为。^[5]

激酶实验：^[1]	- 合并体外HDAC活性 : 从每种乳腺癌细胞系(MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51,或 SK-BR-3)中准备全部细胞提取物。在溶于0.1% (v/v)乙醇的不同浓度Trichostatin A存在时，或者使用0.1% (v/v)乙醇作为对照, 20 μL 细胞粗提取物(~2.5 ×10⁵ 个细胞)与 1 μL ~1.5 × 10⁶ cpm [³H]乙酰标记的组蛋白 H4 肽底物(第2-20位NH2-末端残基) 在25^oC下温育60分钟。使用50 μL 1 M HCl/0.16 M 乙酸对每组 200-μL 反应进行淬火，然后使用600 μL 乙酸乙酯进行抽提，通过闪烁计数器测量释放的[³H]乙酸。使用非线性回归曲线拟合抑制数据，绘制合适的剂量反应曲线，而测定IC50。
细胞实验：^[1]	- 合并 Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3 Concentrations: 溶于无水酒精,终浓度为~10 μM Incubation Time: 96小时 Method: 使用不同浓度Trichostatin A处理细胞96小时。处理后,使用sulforhodamine B 比色分析测定细胞增殖。通过台酚蓝染色排除法测定细胞活力。 (Only for Reference)
动物实验：^[1]	- 合并 Animal Models: 携带NMU诱导的肿瘤的近交系(Ludwig/Wistar/Olac)处女鼠 Dosages: ~5 mg/kg/day Administration: 皮下注射 (Only for Reference)

参考文献

- 合并

溶解度 (25°C)

体外	DMSO	23 mg/mL (76.05 mM)
	Water	Insoluble
	Ethanol	Insoluble
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+2% Tween 80+ddH2O	1.5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Trichostatin A (TSA) SDF

分子量	302.4
化学式	C₁₇H₂₂N₂O₃
CAS号	58880-19-6
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	N/A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04606771	Recruiting	Drug: Osimertinib + Savolitinib\|Drug: Savolitinib + Placebo	Non-Small Cell Lung Cancer	AstraZeneca	September 28 2020	Phase 2
NCT03901053	Recruiting	Device: Reaktiv AFO\|Device: PhatBrace AFO	Foot Injuries and Disorders	Jason Wilken\|Minneapolis Veterans Affairs Medical Center\|Walter Reed National Military Medical Center\|Henry M. Jackson Foundation for the Advancement of Military Medicine\|Center for Veterans Research and Education\|University of Delaware\|Johns Hopkins Bloomberg School of Public Health\|University of Iowa	February 27 2020	Not Applicable
NCT03887650	Recruiting	Drug: Liposomal Bupivicaine 1.3%\|Drug: Bupivacaine 0.5%	Post-operative Pain\|Total Shoulder Arthroplasty\|Osteoarthritis of the Shoulder\|Pain Management	Hartford Hospital	January 14 2019	Phase 4

技术支持

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操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?
回答：
Our S1045 Trichostatin A (TSA) is R enantiomer.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

选择性强的HDAC抑制剂

Rocilinostat (ACY-1215) : HDAC5-selective, IC50=5 nM.
活性最强的HDAC抑制剂

Quisinostat (JNJ-26481585) : HDAC1, IC50=0.11 nM; HDAC2, IC50=0.33 nM.
FDA批准的HDAC抑制剂

Vorinostat (SAHA, MK0683) : Approved by FDA against cutaneous T cell lymphoma.
最新的HDAC抑制剂

RG2833 (RGFP109) ^New : Brain-penetrant HDAC inhibitor with IC50 of 60 nM and 50 nM for HDAC1 and HDAC3, respectively.

研究领域

产品类型

定制您的化合物库

Trichostatin A (TSA)

客户使用Selleck生产的Trichostatin A (TSA)发表文献196篇：

产品安全说明书

HDAC抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Trichostatin A (TSA) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-09-06)

技术支持

常见问题及建议解决方法

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

相关HDAC产品