Trichostatin A (TSA)

For research use only. Not for use in humans.

目录号：S1045 中文名称：曲古柳菌素A

CAS No. 58880-19-6

Trichostatin A (TSA)是一种HDAC抑制剂，无细胞试验中IC50为1.8 nM左右。

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2mg	RMB 2022.93	现货
5mg	RMB 5053.26	现货
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客户使用Selleck生产的Trichostatin A (TSA)发表文献185篇：

Cell, 2019, 178(5):1115-1131

PubMed: 31442404 ( click the link to review the publication )

Cell Metab, 2019, 29(4):886-900

PubMed: 30661930 ( click the link to review the publication )

Nature, 2017, 551(7679):247-250

PubMed: 29088702 ( click the link to review the publication )

Cell, 2017, 171(4):824-835.e18

PubMed: 29056338 ( click the link to review the publication )

Nat Biotechnol, 2015, 10.1038/nbt.3130

PubMed: 25751058 ( click the link to review the publication )

Cancer Cell, 2014, 26(4):534-48

PubMed: 25314079 ( click the link to review the publication )

Mol Cell, 2021, S1097-2765(21)00051-4

PubMed: 33651988 ( click the link to review the publication )

Proc Natl Acad Sci U S A, 2021, 118(22)e2014681118

PubMed: 34035164 ( click the link to review the publication )

Theranostics, 2021, 11(8):3760-3780

PubMed: 33664860 ( click the link to review the publication )

Cell Rep, 2021, 34(3):108646

PubMed: 33472062 ( click the link to review the publication )

Cancer Lett, 2021, 513:36-49

PubMed: 33991616 ( click the link to review the publication )

J Pathol, 2021, 10.1002/path.5657

PubMed: 33638154 ( click the link to review the publication )

Cell Death Dis, 2021, 12(6):532

PubMed: 34031358 ( click the link to review the publication )

Cell Death Dis, 2021, 12(4):405

PubMed: 33854041 ( click the link to review the publication )

Neoplasia, 2021, 23(1):129-139

PubMed: 33316537 ( click the link to review the publication )

Sci China Life Sci, 2021, 10.1007/s11427-020-1855-4

PubMed: 33420926 ( click the link to review the publication )

Mol Cell Biochem, 2021, 10.1007/s11010-021-04050-3

PubMed: 33515198 ( click the link to review the publication )

Nat Metab, 2021, 3(1):75-89

PubMed: 33462516 ( click the link to review the publication )

Sci Adv, 2021, 7(9)eabe5518

PubMed: 33627431 ( click the link to review the publication )

Cell Rep, 2021, 34(3):108646

PubMed: 33472062 ( click the link to review the publication )

Autophagy, 2020, 10.1080/15548627.2019.1709762

PubMed: 31986961 ( click the link to review the publication )

Autophagy, 2020, 15:1-13

PubMed: 32264736 ( click the link to review the publication )

Theranostics, 2020, 10(21):9644-9662

PubMed: 32863951 ( click the link to review the publication )

Theranostics, 2020, 10(15):6790-6805

PubMed: 32550904 ( click the link to review the publication )
Cell Rep, 2020, 33(11):108507

PubMed: 33326790 ( click the link to review the publication )

mBio, 2020, 11(5)e02343-20

PubMed: 33024042 ( click the link to review the publication )

Free Radic Biol Med, 2020, 10.1016/j.freeradbiomed.2020.01.016

PubMed: 31972340 ( click the link to review the publication )

Cell Death Dis, 2020, 11(9):765

PubMed: 32943605 ( click the link to review the publication )

Signal Transduct Target Ther, 2020, 13;5(1):53

PubMed: 32398667 ( click the link to review the publication )

Cells, 2020, 21;9(5):E1280

PubMed: 32455774 ( click the link to review the publication )

Front Immunol, 2020, 3;11:36

PubMed: 32117228 ( click the link to review the publication )

Mol Cancer Ther, 2020, molcanther.0417.2020

PubMed: 33277442 ( click the link to review the publication )

Mol Neurobiol, 2020, 10.1007/s12035-019-01812-5

PubMed: 31919777 ( click the link to review the publication )

Am J Cancer Res, 2020, 10(2):523-535

PubMed: 32195024 ( click the link to review the publication )

Oncogenesis, 2020, 13;9(5):48

PubMed: 32404892 ( click the link to review the publication )

Mol Cancer Res, 2020, 10.1158/1541-7786.MCR-19-0669

PubMed: 32238439 ( click the link to review the publication )

Cancer Biol Med, 2020, 17(4):953-969

PubMed: 33299646 ( click the link to review the publication )

Immunol Cell Biol, 2020, 98(4):318-331

PubMed: 31950542 ( click the link to review the publication )

Front Chem, 2020, 23;8:184

PubMed: 32266211 ( click the link to review the publication )

J Immunol Res, 2020, 2020:7263602

PubMed: 33062724 ( click the link to review the publication )

BMC Microbiol, 2020, 20(1):28

PubMed: 32028887 ( click the link to review the publication )

Arch Insect Biochem Physiol, 2020, 103(4):e21649

PubMed: 31777104 ( click the link to review the publication )

Cell Rep, 2020, 30(7):2195-2208

PubMed: 32075759 ( click the link to review the publication )

Theranostics, 2020, 10(17):7747-7757

PubMed: 32685017 ( click the link to review the publication )

Theranostics, 2020, 10(5):2188-2200

PubMed: 32104503 ( click the link to review the publication )

Mol Cell, 2019, 73(4):788-802

PubMed: 30704899 ( click the link to review the publication )

Mol Cell, 2019, 74(6):1250-1263

PubMed: 31054974 ( click the link to review the publication )

Nat Commun, 2019, 10(1):5792

PubMed: 31857589 ( click the link to review the publication )
Nat Commun, 2019, 10(1):4353

PubMed: 31554795 ( click the link to review the publication )

Nat Commun, 2019, 10(1):5800

PubMed: 31863007 ( click the link to review the publication )

Nucleic Acids Res, 2019, 19;47(16):8502-8520

PubMed: 31616951 ( click the link to review the publication )

Autophagy, 2019, 1-13

PubMed: 30957628 ( click the link to review the publication )

Autophagy, 2019, 10.1080/15548627.2019.1707488

PubMed: 31878840 ( click the link to review the publication )

PLoS Biol, 2019, 17(10):e3000461

PubMed: 31600191 ( click the link to review the publication )

Cancer Res, 2019, 10.1158/0008-5472.CAN-18-2486

PubMed: 31253668 ( click the link to review the publication )

J Cell Biol, 2019, 218(1):267-284

PubMed: 30538141 ( click the link to review the publication )

J Cell Biol, 2019, 218(12):4017-4029

PubMed: 31645458 ( click the link to review the publication )

Cell Death Differ, 2019, 26(5):843-859

PubMed: 29988076 ( click the link to review the publication )

Int J Cancer, 2019, 10.1002/ijc.32425

PubMed: 31111958 ( click the link to review the publication )

Mol Ther, 2019, 27(5):1039-1050

PubMed: 30852137 ( click the link to review the publication )

EBioMedicine, 2019, 43:238-252

PubMed: 31047858 ( click the link to review the publication )

J Bone Miner Res, 2019, 10.1002/jbmr.3716

PubMed: 31112333 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):428

PubMed: 31665064 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):84

PubMed: 30777099 ( click the link to review the publication )

J Exp Clin Cancer Res, 2019, 38(1):463

PubMed: 31718704 ( click the link to review the publication )

J Mol Cell Biol, 2019, 10.1093/jmcb/mjz093

PubMed: 31560394 ( click the link to review the publication )

Mol Cancer Ther, 2019, 18(5):900-908

PubMed: 30824609 ( click the link to review the publication )

Mol Neurobiol, 2019, 10.1007/s12035-019-1565-7

PubMed: 30963442 ( click the link to review the publication )

J Mol Med (Berl), 2019, 97(4):541-552

PubMed: 30806715 ( click the link to review the publication )

Pharmacol Res, 2019, 146:104281

PubMed: 31125601 ( click the link to review the publication )

Cancer Sci, 2019, 110(11):3442-3452

PubMed: 31432592 ( click the link to review the publication )

J Neurochem, 2019, 10.1111/jnc.14935

PubMed: 31811660 ( click the link to review the publication )
J Virol, 2019, 93(4)e01896-18

PubMed: 30518648 ( click the link to review the publication )

Front Cell Neurosci, 2019, 13:468

PubMed: 31708743 ( click the link to review the publication )

Sci Rep, 2019, 9(1):1897

PubMed: 30760778 ( click the link to review the publication )

Sci Rep, 2019, 9(1):4360

PubMed: 30867438 ( click the link to review the publication )

PLoS Comput Biol, 2019, 15(2):e1006826

PubMed: 30785874 ( click the link to review the publication )

Front Genet, 2019, 10:422

PubMed: 31130994 ( click the link to review the publication )

Life Sci, 2019, 223:1-8

PubMed: 30862568 ( click the link to review the publication )

J Cell Biochem, 2019, 10.1002/jcb.29470

PubMed: 31692090 ( click the link to review the publication )

Cell Adh Migr, 2019, 13(1):285-292

PubMed: 31271097 ( click the link to review the publication )

Alcohol Clin Exp Res, 2019, 43(5):822-832

PubMed: 30860602 ( click the link to review the publication )

Oncol Rep, 2019, 41(6):3209-3218

PubMed: 31002353 ( click the link to review the publication )

Int J Mol Med, 2019, 44(5):1789-1800

PubMed: 31545402 ( click the link to review the publication )

Int J Mol Med, 2019, 43(1):285-293

PubMed: 30387821 ( click the link to review the publication )

Tuberculosis (Edinb), 2019, 118:101861

PubMed: 31526947 ( click the link to review the publication )

ACS Omega, 2019, 4(22):19895-19904

PubMed: 31788622 ( click the link to review the publication )

Reprod Fertil Dev, 2019, 31(3):473-481

PubMed: 30301509 ( click the link to review the publication )

Res Vet Sci, 2019, 126:207-212

PubMed: 31610471 ( click the link to review the publication )

Exp Ther Med, 2019, 17(6):4547-4553

PubMed: 31186678 ( click the link to review the publication )

bioRxiv, 2019, 10.1101/748129

PubMed: N/A ( click the link to review the publication )

Sci Adv, 2019, 5(3):eaav1118

PubMed: 30944854 ( click the link to review the publication )

Nat Biomed Eng, 2018, 2(8):578-588

PubMed: 31015631 ( click the link to review the publication )

J Clin Invest, 2018, 128(9):4098-4114

PubMed: 30124467 ( click the link to review the publication )

Cancer Res, 2018, 78(20):5731-5740

PubMed: 30135193 ( click the link to review the publication )

Cell Rep, 2018, 24(7):1722-1729

PubMed: 30110629 ( click the link to review the publication )
Mol Ther, 2018, 26(7):1828-1839

PubMed: 29730197 ( click the link to review the publication )

Oncogene, 2018, 37(8):1049-1061

PubMed: 29084211 ( click the link to review the publication )

Oncogene, 2018, 37(50):6414-6424

PubMed: 30068939 ( click the link to review the publication )

Cancer Lett, 2018, 432:121-131

PubMed: 29890207 ( click the link to review the publication )

Mol Oncol, 2018, 12(5):724-755

PubMed: 29577611 ( click the link to review the publication )

Oncogenesis, 2018, 7(11):91

PubMed: 30467308 ( click the link to review the publication )

Sci Rep, 2018, 8(1):8492

PubMed: 29855565 ( click the link to review the publication )

J Proteome Res, 2018, 17(4):1436-1451

PubMed: 29564889 ( click the link to review the publication )

Plant Cell Rep, 2018, 37(1):115-123

PubMed: 28939922 ( click the link to review the publication )

Exp Cell Res, 2018, 371(1):231-237

PubMed: 30107147 ( click the link to review the publication )

Int Immunopharmacol, 2018, 65:303-311

PubMed: 30342347 ( click the link to review the publication )

Oncol Rep, 2018, 39(4):1892-1900

PubMed: 29393444 ( click the link to review the publication )

Biochem Biophys Res Commun, 2018, 503(2):420-427

PubMed: 29649477 ( click the link to review the publication )

Cell Biochem Funct, 2018, 36(8):398-407

PubMed: 30484863 ( click the link to review the publication )

Med Sci Monit, 2018, 24:461-472

PubMed: 29363667 ( click the link to review the publication )

Methods Mol Biol, 2018, 1755:179-195

PubMed: 29671271 ( click the link to review the publication )

Curr Protoc Pharmacol, 2018, 81(1):e41

PubMed: 29927058 ( click the link to review the publication )

Sci Rep, 2018, 8(1):13072

PubMed: 30166563 ( click the link to review the publication )

Cell Res, 2017, 27(7):898-915

PubMed: 28497810 ( click the link to review the publication )

Mol Cell, 2017, 67(4):566-578

PubMed: 28803781 ( click the link to review the publication )

Cell Mol Immunol, 2017, 14(4):371-379

PubMed: 26388239 ( click the link to review the publication )

Oncogene, 2017, 36(12):1707-1720

PubMed: 27694895 ( click the link to review the publication )

Plant Physiol, 2017, 175(3):1484-1496

PubMed: 28972079 ( click the link to review the publication )

Chemistry, 2017, 23(13):3107-3116

PubMed: 27922200 ( click the link to review the publication )
Cancer Epidemiol Biomarkers Prev, 2017, 26(9):1411-1419

PubMed: 28619831 ( click the link to review the publication )

Eur J Med Chem, 2017, 127:531-553

PubMed: 28109947 ( click the link to review the publication )

Sci Rep, 2017, 7:46195

PubMed: 28387374 ( click the link to review the publication )

J Biol Chem, 2017, 292(31):12842-12859

PubMed: 28634230 ( click the link to review the publication )

J Pharmacol Exp Ther, 2017, 363(2):211-220

PubMed: 28860353 ( click the link to review the publication )

J Diabetes Res, 2017, 2017:8208065

PubMed: 28191472 ( click the link to review the publication )

Int J Parasitol Drugs Drug Resist, 2017, 7(1):51-60

PubMed: 28110187 ( click the link to review the publication )

PLoS One, 2017, 12(10):e0186620

PubMed: 29045501 ( click the link to review the publication )

Connect Tissue Res, 2017, 58(1):64-75

PubMed: 27404795 ( click the link to review the publication )

Biochem Biophys Res Commun, 2017, 485(2):454-460

PubMed: 28192116 ( click the link to review the publication )

Oncotarget, 2017, 8(11):17995-18009

PubMed: 28152508 ( click the link to review the publication )

Oncotarget, 2017, 8(7):11937-11949

PubMed: 28060760 ( click the link to review the publication )

Bioorg Med Chem, 2017, 25(7):2105-2132

PubMed: 28259528 ( click the link to review the publication )

Nat Commun, 2016, 7:10690

PubMed: 26891683 ( click the link to review the publication )

Nucleic Acids Res, 2016, 45(3):1159-1176

PubMed: 28180300 ( click the link to review the publication )

Clin Cancer Res, 2016, 22(8):1978-88

PubMed: 26634271 ( click the link to review the publication )

Thorax, 2016, 633-45

PubMed: 27071418 ( click the link to review the publication )

EMBO Rep, 2016, 17(7):999-1012

PubMed: 27154402 ( click the link to review the publication )

J Med Chem, 2016, 59(4):1613-33

PubMed: 26681404 ( click the link to review the publication )

PLoS Pathog, 2016, 12(10):e1005950

PubMed: 27764245 ( click the link to review the publication )

Free Radic Biol Med, 2016, 99:167-178

PubMed: 27498117 ( click the link to review the publication )

Oxid Med Cell Longev, 2016, 2016:4085727

PubMed: 27746856 ( click the link to review the publication )

FEBS J, 2016, 283(20):3739-3754

PubMed: 27573079 ( click the link to review the publication )

Am J Pathol, 2016, 186(10):2701-8

PubMed: 27555113 ( click the link to review the publication )
Mol Cell Biol, 2016, 36(22):2838-2854

PubMed: 27573019 ( click the link to review the publication )

Int J Oncol, 2016, 48(6):2591-607

PubMed: 27082124 ( click the link to review the publication )

BMC Cancer, 2016, 16(1):886

PubMed: 27842508 ( click the link to review the publication )

Mol Cell Biochem, 2016, 415(1-2):197-205

PubMed: 27000860 ( click the link to review the publication )

Oncotarget, 2016, 8(60):100975-100988

PubMed: 29254139 ( click the link to review the publication )

Genet Mol Res, 2016, 15(4)

PubMed: 27819724 ( click the link to review the publication )

Sci Rep, 2016, 6:21678

PubMed: 26902929 ( click the link to review the publication )

Tumour Biol, 2016, 37(6):8293-304

PubMed: 26729194 ( click the link to review the publication )

Tumour Biol, 2016, 37(8):10269-78

PubMed: 26831669 ( click the link to review the publication )

Nat Commun, 2015, 3;6:10091

PubMed: 26631872 ( click the link to review the publication )

EMBO Mol Med, 2015, 10.15252/emmm.201404396

PubMed: 25872941 ( click the link to review the publication )

Cancer Lett, 2015, 356(2 Pt B):828-36

PubMed: 25449774 ( click the link to review the publication )

Am J Transplant, 2015, 10.1111/ajt.13106

PubMed: 25708614 ( click the link to review the publication )

Cell Death Dis, 2015, 6:e1586

PubMed: 25569103 ( click the link to review the publication )

J Antimicrob Chemother, 2015, 10.1093/jac/dku549

PubMed: 25558076 ( click the link to review the publication )

Mol Nutr Food Res, 2015, 59(2):189-202

PubMed: 25303559 ( click the link to review the publication )

Mol Nutr Food Res, 2015, 59(2):189-202

PubMed: ( click the link to review the publication )

Am J Physiol Lung Cell Mol Physiol, 2015, 309(12):L1410-9

PubMed: 26498249 ( click the link to review the publication )

Chem Biol Interact, 2015, 242:227-34

PubMed: 26482938 ( click the link to review the publication )

J Appl Physiol (1985), 2015, 119(4):342-51

PubMed: 26112243 ( click the link to review the publication )

PLoS One, 2015, 10(3):e0120587

PubMed: 25807249 ( click the link to review the publication )

Oncotarget, 2015, 6(18):15814-27

PubMed: 26158412 ( click the link to review the publication )

EMBO J, 2014, 34(1):115-29

PubMed: 25398909 ( click the link to review the publication )

Proc Natl Acad Sci USA, 2014, 111(3):E364-73

PubMed: 24395801 ( click the link to review the publication )
PLoS Biol, 2014, 12(1):e1001758

PubMed: 24409098 ( click the link to review the publication )

Cancer Lett, 2014, 10.1016/j.canlet.2014.10.034

PubMed: ( click the link to review the publication )

Development, 2014, 141(7):1534-43

PubMed: 24598165 ( click the link to review the publication )

Mol Cell Biol, 2014, 34(22):4143-64

PubMed: 25202123 ( click the link to review the publication )

Toxicol Appl Pharmacol, 2014, 274(1):7-16

PubMed: 24200994 ( click the link to review the publication )

Life Sci, 2014, 99(1-2):31-6

PubMed: 24486299 ( click the link to review the publication )

Biochem Biophys Res Commun, 2014, 445(2):320-6

PubMed: 24530917 ( click the link to review the publication )

Exp Hematol, 2014, 42(7):574-80

PubMed: 24607957 ( click the link to review the publication )

Oncotarget, 2014, 5(16):6701-15

PubMed: 25149533 ( click the link to review the publication )

Sci Rep, 2014, 4:4663

PubMed: 24722541 ( click the link to review the publication )

Science, 2014, 344(6183):1252304

PubMed: 24727982 ( click the link to review the publication )

Neuropsychopharmacology, 2013, 38(9):1674-84

PubMed: 23474591 ( click the link to review the publication )

Plant J, 2013, 74(5):815-28

PubMed: 23464703 ( click the link to review the publication )

Cytokine, 2013, 65(2):121-5

PubMed: 24373940 ( click the link to review the publication )

Genes Brain Behav, 2013, 13(1):69-86

PubMed: 24286462 ( click the link to review the publication )

Epigenetics, 2012, 7(10):1161-72

PubMed: 22965008 ( click the link to review the publication )

Adv Genet, 2010, 70:341-86

PubMed: 20920755 ( click the link to review the publication )

产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述

Trichostatin A (TSA)是一种HDAC抑制剂，无细胞试验中IC50为1.8 nM左右。

靶点

HDAC ^[1]
(Cell-free assay)

~1.8 nM

体外研究

Trichostatin A 抑制八种乳腺癌细胞系，包括MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3增殖，平均IC50为124.4 nM (范围为26.4-308.1 nM),作用于表达ERα的细胞系比作用于表达 ERα阴性的细胞系更有效。Trichostatin A作用于全部乳腺癌细胞系，抑制HDAC活性，平均IC50为 2.4 nM (范围为0.6-2.6 nM), 产生显著的组蛋白 H4高度乙酰化。^[1]与 Trapoxin (TPX) 和 Chlamydocin 有效抑制HDAC1 或 HDAC4而不是 HDAC6不同，Trichostatin A 抑制这些HDACs，且抑制程度相似， IC50 分别为6 nM, 38 nM, 和8.6 nM。^[2] Trichostatin A (100 ng/mL) 作用于MIA PaCa-2细胞，通过招募p300和 PCAF进入Sp1-NF-Y HDAC复合体，而诱导转化生长因子βⅡ型受体(TβRII) 表达，复合体与TβRII 启动子的DNA片段结合，伴随着Sp1乙酰化，且与复合体相关的HDAC数理全部降低。^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
HeLa	M3:0eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NEnMVVcyOC9\|MD:1NEBvVQ>?	NV\ybndvPzJiaB?=	NHP0OIxFVVOR	NWPv[XZMcW6lcnXhd4V{KHSqZTDueY1j\XJib3[gUW1RKCkQlN8ocUkhdG:\|czDj[YxteyCmb4PlJIRmeGWwZHXueIx6	NFfW[5Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G2OVc1QCd-MkOxOlU4PDh:L3G+
HeLa	NHrlVIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MnGzNlAhdk1?	MmWzO\|IhcA>?	MlH0SG1UVw>?	M1HOTGlEPTBib3[gNlBvVQ>?	NEHwWmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G2OVc1QCd-MkOxOlU4PDh:L3G+
HeLa	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4r3e\|QxKG6P	MUm0PEBp	M1nHTmROW09?	MnfiTWM2OCCxZjC0NI5O	NFS1Nm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G2OVc1QCd-MkOxOlU4PDh:L3G+
HeLa	M2Tlemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MXexNFAhdk1?	NF\1Uo8zPCCq	NVi5SWc6TE2VTx?=	MVfJR\|UxKG:oIEGwNI5O	M3HWNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MU[1O\|Q5Lz5{M{G2OVc1QDxxYU6=
Caco-2	NVjPeJk{TnWwY4Tpc44hSXO\|YYm=	MUKxJOK2VcLi	NWTaUGVVOjRiaB?=		NHnYdlZl\WO{ZXHz[ZMhW0WUVDDwdo91\WmwIHX4dJJme3Orb36=	NGOyWZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G5OVA4OCd-MkOxPVUxPzB:L3G+
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HLEB3	M2D0TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NVGzRodtOC52L{CuPEDPxE1?	MYm0PEBp	NEnRblhFVVOR	NIO2NVVqdmS3Y3XzJINmdGxiYYDvdJRwe2m\|	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF3N{i3PEc,OjRzNUe4O\|g9N2F-
SRA01/04	NFHWOYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NXnUeG1wOC52L{CuPEDPxE1?	NFXyNY41QCCq	NUnvb3o2TE2VTx?=	MoXjbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>?	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF3N{i3PEc,OjRzNUe4O\|g9N2F-
HLEB3	MojGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3POWlAvOiEQvF2=	MnnwOFghcA>?	NHf4TWpFVVOR	Mluyd5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hfGi{b4XnbEB{fXCycnXzd4lv\yC2aHWgVGk{Uy:Da4SsJJA{QE2DUFugZY5lKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfZM>	Mn;qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNUe4O\|goRjJ2MUW3PFc5RC:jPh?=
SRA01/04	NIjpbpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4q4PVAvOiEQvF2=	MlrnOFghcA>?	MYTEUXNQ	M4jxd5N2eHC{ZYPz[ZMh[2WubDDwdo9tcW[ncnH0bY9vKHSqcn;1[4ghe3WycILld5NqdmdidHjlJHBKO0txQXv0MEBxOziPQWDLJIFv\CCHUluxM\|Ihe2mpbnHsbY5oKHCjdHj3ZZl{	NXX2N\|I6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
A2780	NXHsZpBOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NILMem8xNjB3LUKg{txO	M1vLRVExNzJ2L{S4JIg>	NFjCdHBFVVOR	MVPt[YRq[XSnczDndo94fGhiYYLy[ZN1KGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>?	M3HYRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkKzPFAyLz5{NEKyN\|gxOTxxYU6=
SKOV3	NInlUmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NHnQflgxNjB3LUKg{txO	MVSxNE8zPC92ODDo	M1vlTGROW09?	MXLt[YRq[XSnczDndo94fGhiYYLy[ZN1KGmwIHGgZ49v[2WwdILheIlwdi1iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>?	MkLkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkO4NFEoRjJ2MkKzPFAyRC:jPh?=
Huh7	M3m3cmZ2dmO2aX;uJGF{e2G7	MX[wMlEwOC53L{GuNEDPxE1?	NFLlV2QzPGh?		M3mxXpN2eHC{ZYPz[ZMhYVSKRFOyJIdmdmViZYjwdoV{e2mxbh?=	NHHzbmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2PVY4Oid-MkSyOlk3PzJ:L3G+
hMSCs	MWDGeY5kfGmxbjDBd5NigQ>?	MVS2MlI2KG6P	NWDaNYxWOjRiaB?=	M{i4O2ROW09?	NUW5NXZMe3SjYnnsbZpmeyCKaYP0c45mKEGlZYT5cIF1cW:wIHHu[EB1cGViRYjwdoV{e2mxbjDv[kBRdHW{aYDveIVvfCCJZX7ldy=>	M{DNNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{GyN\|U3Lz5{NEOxNlM2PjxxYU6=
HT29	MlLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MkfxNVgxKG6P	NYe0NpF4PzJiaB?=		NFnqN\|BKSzVyIH;mJFE5OCCwTR?=	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4OEK2OUc,OjR\|NkiyOlU9N2F-
RPE	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NGG5PZMxNjJxMD60M\|AvQC9zIN88US=>	MkjNNlQwPDhxN{KgbC=>	MWDEUXNQ	NHX1fFlqdmirYnn0d{B1cGVicILvcIln\XKjdHnvckBjgSClZXzsJIN6[2ynIHHydoV{fA>?	Mm[5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NU[2NFIoRjJ2NEW2OlAzRC:jPh?=
U87 GBM	Mk[ySpVv[3Srb36gRZN{[Xl?	M4r6SFExOC93MECgUo0>	NHXpNlI1QCCq	M{\EPVExOCViZYToZY5wdA>?	MYfJcoR2[2W\|IGPlcoV{[2WwY3WtUIls\SCDbITldoF1cW:wczDpckBPfWOuZXHyJG1wenCqb3zv[5k>	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR4NEi0NUc,OjR2NkS4OFE9N2F-
U87 GBM	MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NFTNOoMyODBxM{CwM\|UxOCCwTR?=	Mlm2O\|IhcA>?	M33UVVExOCViZYToZY5wdA>?	NF\Kfldz\WS3Y3XzJI1m[W5iY3XscEBvfW2kZYKgZpkhOzFuIEW0MEBidmRiNUlCpEU>	NFH2WmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES2OFg1OSd-MkS0OlQ5PDF:L3G+
ACP03	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M320eFI2OC9\|NUCvOVAxdk1?	MWSyOIg>		M4\ab4Rm[3KnYYPld{Bk\WyuII\pZYJqdGm2eTDv[kBieHC{b4jpcYF1\Wy7IEewxsAmKGG2IEK1NOKhdk1?	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ4OEW0O{c,OjR4Nki1OFc9N2F-
ACP02	MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4DTeFI2OC9\|NUCvOVAxdk1?	M1nhcVI1cA>?		NV;aOFBS\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JI9nKGGycILvfIlu[XSnbImgO\|DDqCViYYSgNlUxyqCwTR?=	MofSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4Nki1OFcoRjJ2Nk[4OVQ4RC:jPh?=
HeLa	NULJb41GTnWwY4Tpc44hSXO\|YYm=	NXzieoFlOW2P	NITkdFcxNjWq		M1nCZYFjd2yrc3jld{B1cGVidH;0ZYwhUESDQzDhZ5Rqfmm2eR?=	NX7LcXlHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NFc1PzRpPkK0O\|A4PDd2PD;hQi=>
HMEC-1	M{jCUGZ2dmO2aX;uJGF{e2G7	MoToN\|Axdk1?	M2TybVI1cA>?		NX3SSG9QcW6lcnXhd4V{KGW6cILld5Nqd25ib3dCpHZGT0[UM9MgcXJPSQ>?	MoC1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5MUC2N\|EoRjJ2N{GwOlMyRC:jPh?=
HeLa	MnOzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXzPOY92Os7:TR?=	NUPtV\|RUPDiq		MUjk[YNz\WG\|ZYOgZ4VtdCC4aXHibYxqfHliYXLveZQhOjVn	NY[2OGRZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4OFYyOzVpPkK0PFQ3OTN3PD;hQi=>
LNCaP	M1jLTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NX\GPVl4OTByLUGwNFBvVQ>?	NYX1WZZpOjSq		M33rUWlEPTBib3[gN\|Axdk1?	NETySHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi1OFY2QCd-MkS4OVQ3PTh:L3G+
PC3	NWW1Wnh2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MX:xNFAuOTByMH7N	MkLONlRp		NGTWS\|dKSzVyIH;mJFMxOG6P	M4e0O\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEW0OlU5Lz5{NEi1OFY2QDxxYU6=
CNE2	NFLLWZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVOxNFAuPjBybl2=	NXnmVldPOjRxNEivO\|Jp		NXLNRohwcW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTD0bY1mNSCjbnSg[I9{\S2mZYDlcoRmdnRibXHucoVz	NYPHOIx4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5Olk6ODFpPkK0PVY6QTBzPD;hQi=>
HeLa	MYDGeY5kfGmxbjDBd5NigQ>?	NYXNOI94OjVybl2=	M{nrT\|E3cA>?	Mlm0SG1UVw>?	NXP2NJl1cW6lcnXhd4V{KEO\UEHBNUBuWk6DIHX4dJJme3Orb39CpC=>	NF7ybVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUGxOlY5QCd-MkWxNVY3QDh:L3G+
A549	NVniUlFST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M3jFNVAvODFvMD65{txO	MVK3Nog>	MUjEUXNQ	NUPLd\|RSUUN3MDDv[kAxNjB4zszN	NHvVfYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2PVQ6OSd-MkWxOlk1QTF:L3G+
SKOV-3	NIjKPGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1SzOlAvODFvMD65{txO	NYDW[JNQPzKq	MXjEUXNQ	NHLYdGxKSzVyIH;mJFAvOzMQvF2=	Mn\MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkm0PVEoRjJ3MU[5OFkyRC:jPh?=
A549	M3vzdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M1zJZlAvOS1zzszN	MXW0PIg>	MVLEUXNQ	NEHSW5pKSzVyIH;mJFAvOjkQvF2=	NYLJOHhmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOlk1QTFpPkK1NVY6PDlzPD;hQi=>
SKOV-3	MnfWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MofkNE4yNTIQvF2=	Ml7QOFhp	MWrEUXNQ	NEDCXmRKSzVyIH;mJFAvP87:TR?=	M3;2b\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
A549	M4rxOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M4myVFEuOTEQvF2=	M1;wV\|I1cA>?	MVLEUXNQ	NInOVJNKSzVyIH;mJFMvOs7:TR?=	MonaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkm0PVEoRjJ3MU[5OFkyRC:jPh?=
SKOV-3	M{flRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYmxMVEx\|ryP	MkewNlRp	MVrEUXNQ	MneyTWM2OCCxZjC1MlbPxE1?	M37DdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
MCF7	Mm\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYTKRlFsOC14MEDuUS=>	M17odFI1cA>?	NYrtT4xlTE2VTx?=	M3nYPGlEPTBib3[gO\|VvVQ>?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF7MkeyNUc,OjVzOUK3NlE9N2F-
MDA-MB-231	MoH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHjwbY0xNTZyMH7N	NEXxSGszPGh?	MX;EUXNQ	Mli0TWM2OCCxZjCxNFBvVQ>?	MmC5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzOUK3NlEoRjJ3MUmyO\|IyRC:jPh?=
A431	MY\GeY5kfGmxbjDBd5NigQ>?	NWr5ToRjPTCwTR?=	MlKzNk83NzF{L{K0bC=>	NHTCe2FFVVOR	MmT5ZYN1cX[jdHXzJJAzOSCjbnSgbY5pcWKrdIOgRXRHOyCneIDy[ZN{cW:w	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN5MUC2PUc,OjV\|N{GwOlk9N2F-
A431	MoWxRZBweHSxc3nzJGF{e2G7	MVyyM\|ExNzVyL{GwNI5O	M2Hid\|Q5cA>?	M2CwOGROW09?	MVTpcohq[mm2czD0bIUh[2WubDDndo94fGh?	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTN5MUC2PUc,OjV\|N{GwOlk9N2F-
PC3	NF22U2lHfW6ldHnvckBCe3OjeR?=	NUfUZYh2OC5zzszN	M{HteFQ5cA>?	MYnEUXNQ	M3jnWolv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ?	MnTIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2M{S5PVcoRjJ3NEO0PVk4RC:jPh?=
SW480	NX\STJkxTnWwY4Tpc44hSXO\|YYm=	MYiwMlHPxE1?	NV3YcZY6PDiq	M1rVOGROW09?	M1nKO4lv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ?	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR\|NEm5O{c,OjV2M{S5PVc9N2F-
PC3	Mlm2SpVv[3Srb36gRZN{[Xl?	NWHoeYl2OC5zzszN	NWHFWW9uPDiq	MW\EUXNQ	MlzwZZR1\W63YYTld{Bqdn[jc3nvckBidmRibXnndoF1cW:w	M2r6W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
PC3	M2POXWZ2dmO2aX;uJGF{e2G7	MX2wMlHPxE1?	M4TWOVQ5cA>?	Mne1SG1UVw>?	MUXy[ZZmenOnczDFUXQ>	NULLcFhnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N\|Q6QTdpPkK1OFM1QTl5PD;hQi=>
SW480	MnXESpVv[3Srb36gRZN{[Xl?	NITnUpkxNjIQvF2=	M3S5[VQ5cA>?	NHTUWGJFVVOR	NWDEfItP[XS2ZX71ZZRmeyCrbo\hd4lwdiCjbnSgcYloemG2aX;u	NWLyNnNqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N\|Q6QTdpPkK1OFM1QTl5PD;hQi=>
SW480	M{nxZWZ2dmO2aX;uJGF{e2G7	NEfiOIkxNjIQvF2=	NVXp[od3PDiq	MnXpSG1UVw>?	M1jtbpJmfmW{c3XzJGVOXA>?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR\|NEm5O{c,OjV2M{S5PVc9N2F-
MEFs	NXzk[\|RvTnWwY4Tpc44hSXO\|YYm=	M4fMUlXPxE1?	M13JUVE3cA>?		M37xZolv[3KnYYPld{B1cGViRWDFR{BifHSjY3jt[Y51NCCWaYKg[IVtcX[ncomgZY5lKHSqZTDl[oZq[2mnbnP5JI9nKHCnZHXzeIFtKG[xcn3heIlwdg>?	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR6Mk[zOEc,OjV2OEK2N\|Q9N2F-
TE13	MXLBdI9xfG:\|aYOgRZN{[Xl?	NU[3bGN1OC5\|zszN	NXvJPFk3OjSq		NX\sNYRicW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbh?=	NF35W3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW3PVY3PSd-MkW1O\|k3PjV:L3G+
TE13	MXHGeY5kfGmxbjDBd5NigQ>?	M1nBc\|AvO87:TR?=	Mn;INlRp		M4LMcZVxNXKnZ4XsZZRmeyCUQWPTSlVCKGyndnXs	NGH0VGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW3PVY3PSd-MkW1O\|k3PjV:L3G+
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HepG2	NVGwU2t{TnWwY4Tpc44h[XO\|YYm=				MUjVdE1z\We3bHH0bY9vKG:oIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCxZjDoeY1idiCFTFGtNUBxem:vb4TldkBz\Werb36gMVExPTVidH:gMVYz[nBidILhcpNn\WO2ZXSgbY4hUGWyR{KgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCHQ{WwJF0hOC5\|ODFOwG0v	NH;kUWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1OlExOid-MkW1OVYyODJ:L3G+
HeLa	NH\JWVNHfW6ldHnvckBie3OjeR?=				NHHPboNKdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC2dXL1cIlvKGSnYXPleJlt[XSrb36gbY5kfWKjdHXkJIZweiCxdnXycolocHRiYomgZ2VNUVODLDDFR\|UxKD1iMD6wNFUzKM7:TT6=	NGfSZXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[xNVkyQSd-Mk[2NVE6OTl:L3G+
HL60	M1zjeGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M3HHWVI1KGi{cx?=		MUfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdHNiYX\0[ZIhOjRiaILzJIJ6KE2WVDDhd5NigSxiR1m1NEA:KDBwNUmg{txONg>?	M{XyeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEW0PVE5Lz5{OUS1OFkyQDxxYU6=
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friend cell	Ml6xSpVv[3Srb36gZZN{[Xl?				Mo\yTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiZoLp[Y5lKGOnbHzzJJBzd2yrZnXyZZRqd25uIFnDOVAhRSByLkC0JO69VS5?	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDZzM{OxNkc,OTR4MUOzNVI9N2F-
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melanoma B16/BL6	NIL6SlRHfW6ldHnvckBie3OjeR?=				NWDiT\|VyUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KGirc4TvcoUh\GWjY3X0fYxie2W\|IDjISGFEOSlicILldIFz\WRiZoLvcUBud3W\|ZTDt[Yxidm:vYTDCNVYwSkx4IHPlcIx{NCCLQ{WwJF0hOC5yMUmg{txONg>?	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
melanoma B16/BL6	MmnGSpVv[3Srb36gZZN{[Xl?				Mn7MTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIhqe3SxbnWg[IVi[2W2eXzhd4UhMEiGQVO0LUBxemWyYYLl[EBnem:vIH3veZNmKG2nbHHuc41iKEJzNj;CUFYh[2WubIOsJGlEPTBiPTCwMlAzKM7:TT6=	Mme5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTVzMEm2NlYoRjF3MUC5OlI3RC:jPh?=
melanoma B16/BL6	M{\Ve2Z2dmO2aX;uJIF{e2G7				M1vs[mlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKDZicILldIFz\WRiZoLvcUBud3W\|ZTDt[Yxidm:vYTDCNVYwSkx4IHPlcIx{NCCLQ{WwJF0hOC5yMkig{txONg>?	NWSwSJJnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWxNFk3OjZpPkG1NVA6PjJ4PD;hQi=>
melanoma B16/BL6	NGOwOnRHfW6ldHnvckBie3OjeR?=				MXfJcohq[mm2b4L5JIFkfGm4aYT5JIFo[Wmwc4SgbIl{fG:wZTDk[YFk\XS7bHHz[UA5KHC{ZYDhdoVlKG[{b32gcY92e2VibXXsZY5wdWFiQkG2M2JNPiClZXzsd{whUUN3MDC9JFAvODRizszNMi=>	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
293T	Mn3ISpVv[3Srb36gZZN{[Xl?				NV;Leo86UW6qaXLpeIlwdiCxZjDoeY1idiCqaYP0c45mKGSnYXPleJlt[XOnIESgdJJmeGG{ZXSg[pJwdSB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6wNkDPxE1w	NXnJToFURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVQzOjRpPkG1OFU1OjJ2PD;hQi=>
293T	MonJSpVv[3Srb36gZZN{[Xl?				NYnTRpdLUW6qaXLpeIlwdiCxZjDoeY1idiCqaYP0c45mKGSnYXPleJlt[XOnIEGgdJJmeGG{ZXSg[pJwdSB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6wNkDPxE1w	M2XRXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEW0NlI1Lz5zNUS1OFIzPDxxYU6=
293T	NXznZZJnTnWwY4Tpc44h[XO\|YYm=				MXrJcohq[mm2aX;uJI9nKGi3bXHuJIhqe3SxbnWg[IVi[2W2eXzhd4UhPiCycnXwZZJm\CCocn;tJFI6O1RiY3XscJMtKEmFNUCgQUAxNjB{IN88UU4>	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR3NEKyOEc,OTV2NUSyNlQ9N2F-
293T	Mn;RSpVv[3Srb36gZZN{[Xl?				Ml\CTY5pcWKrdHnvckBw\iCqdX3hckBpcXO2b37lJIRm[WOndInsZZNmKDhicILldIFz\WRiZoLvcUAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yNDFOwG0v	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR3NEKyOEc,OTV2NUSyNlQ9N2F-
HeLa	MWXGeY5kfGmxbjDhd5NigQ>?				NUDFfng2UW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgbJVu[W5iaHnzeI9v\SCmZXHj[ZR6dGG\|ZTCoTGRCSyliZoLvcUBJ\UyjIHPlcIx{KHerdHigd5Vje3S{YYTlJFViNCCLQ{WwJF0hOC5yNUS1JO69VS5?	NVXYWYJTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVYzPjdpPkG1OFU3OjZ5PD;hQi=>
HCT116	NEXQTFdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				NInUOFZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGhEXDFzNjDj[YxteyxiSVO1NEA:KDBwMECxN{DPxE1w	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjlyNEi5NEc,OTZ7MES4PVA9N2F-
H1299	NXLWTlBESW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				MmTvRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDINVI6QSClZXzsd{whUUN3MDC9JFAvOSEQvF2u	MmW0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MES4PVAoRjF4OUC0PFkxRC:jPh?=
H661	MYfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?				M{TITmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgTFY3OSClZXzsd{whUUN3MDC9JFAvOyEQvF2u	M3S2e\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUC0PFkxLz5zNkmwOFg6ODxxYU6=
H661	Mn3xRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M3rjbFQ5KGi{cx?=		NILvemFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JGg3PjFiY3XscJMh[W[2ZYKgOFghcHK\|IHL5JHhVXCCjc4PhfUwhUUN3MDC9JFAvODh3IN88UU4>	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ{NEe3N{c,OTd4MkS3O\|M9N2F-
WiDr	MVLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?				M2XIe2lvcGmkaYTpc44hd2ZiU1HQNVMxKG2nZHnheIVlKGOnbHyg[5Jwf3SqIHnuJIh2dWGwIGfpSJIh[2WubIOsJGlEPTBiPTCwMlU4PzZizszNMi=>	MnjPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NEOxNVIoRjF5NkSzNVEzRC:jPh?=
U251	MlLZSpVv[3Srb36gZZN{[Xl?				M4jCXmlvcGmkaYTpc44hd2ZiU1HQNVMxKGmwIG\FS2Yue3SrbYXsZZRm\CCqdX3hckBWOjVzIHPlcIx{KGK7IGDMRXAhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwNkGxNkDPxE1w	M1HNOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkSzNVEzLz5zN{[0N\|EyOjxxYU6=
293T	NEm1UIZHfW6ldHnvckBie3OjeR?=				Mlv6TY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTDlfJBz\XO\|ZXSgbY4hOjl\|VDDj[YxteyxiSVO1NEA:KDBwMECxPUDPxE1w	NWXuXmxPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe4PFEzOzJpPkG3PFgyOjN{PD;hQi=>
293T	MWjGeY5kfGmxbjDhd5NigQ>?				NV;xOJJRUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNFAzKM7:TT6=	MoLCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OEGyN\|IoRjF5OEixNlMzRC:jPh?=
293T	MVLGeY5kfGmxbjDhd5NigQ>?				M1nzemlvcGmkaYTpc44hd2ZibX;1d4UhUESDQ{[g[ZhxemW\|c3XkJIlvKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkCwNlgh\|ryPLh?=	Mo[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OEGyN\|IoRjF5OEixNlMzRC:jPh?=
H661	NVG2bXdiSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NUfBephLPDhiaILz		NYjN[FJzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBJPjZzIHPlcIx{KGGodHXyJFQ5KGi{czygTWM2OCB;IECuNFg2KM7:TT6=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh7N{iyOEc,OTd6OUe4NlQ9N2F-
293T	M1\Xb2Z2dmO2aX;uJIF{e2G7				MVrJcohq[mm2aX;uJI9nKEiGQVOxJIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6xPUDPxE1w	NELwO3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mwNFkyOSd-MUe5NFA6OTF:L3G+
293T	M3fwNWZ2dmO2aX;uJIF{e2G7				Ml7KTY5pcWKrdHnvckBw\iCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNkDPxE1w	MmnEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7MEC5NVEoRjF5OUCwPVEyRC:jPh?=
293T	NH7CVFJHfW6ldHnvckBie3OjeR?=				MlXYTY5pcWKrdHnvckBw\iCKRFHDOkBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNlgh\|ryPLh?=	M4PGdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUCwPVEyLz5zN{mwNFkyOTxxYU6=
NIH3T3	NUjwboxZTnWwY4Tpc44h[XO\|YYm=				NE\wXoVKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWMyKGW6cILld5Nm\CCrbjDOTWg{XDNiY3XscJMtKEmFNUCgQUAxNjB{MTFOwG0v	NX;v[ZU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5Nlk4QThpPkG3PVI6Pzl6PD;hQi=>
human 293	MUPGeY5kfGmxbjDhd5NigQ>?				M1XKXWlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR\|Qh\XiycnXzd4VlKGmwIEK5N{Bk\WyuczygTWM2OCB;IECuNFM1KM7:TT6=	MkjnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7Mkm3PVgoRjF5OUK5O\|k5RC:jPh?=
mouse 293	MY\GeY5kfGmxbjDhd5NigQ>?				MYXJcohq[mm2aX;uJI9nKG2xdYPlJJJm[2:vYnnuZY51KEiGQVO2JIV5eHKnc4Pl[EBqdiB{OUOgZ4VtdHNuIFnDOVAhRSByLkC4NUDPxE1w	NHjzSYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{myPVc6QCd-MUe5Nlk4QTh:L3G+
SKBR3	NGDqUG1CdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				MkCxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCVS1LSN{Bk\WyuczygTWM2OCB;IECuNFIh\|ryPLh?=	NFrIT4Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
MM96L	NH33VVhHfW6ldHnvckBie3OjeR?=				M4LVeWlvcGmkaYTpc44hd2ZiaIXtZY4hVU17NlygZ4VtdHNuIFnDOVAhRSByLkCzJO69VS5?	M162fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
SNU16	MoPpSpVv[3Srb36gZZN{[Xl?				MYDJcohq[mm2aX;uJI9nKEiGQVOg[pJwdSCqdX3hckBUVlVzNjDj[YxteyxiSVO1NEA:KDBwMEWzJO69VS5?	NFTienc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
A549	NHjuPGFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				NV71Z41OSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzMEBKSzVyIE2gNE4xQCEQvF2u	NI\VVWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
MKN45	NF3pZpVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				M{fCW2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVvOOFUh[2WubIOsJGlEPTBiPTCwMlEh\|ryPLh?=	M3T1XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
SQ20B	MoniSpVv[3Srb36gZZN{[Xl?				NVuyR3RpUW6qaXLpeIlwdiCxZjDoeY1idiCVUUKwRkBk\WyuczygTWM2OCB;IECuNkDPxE1w	MknoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEe1OVQoRjF6MkS3OVU1RC:jPh?=
neonatal foreskin fibroblasts	NX;vTnhbT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=				NUnwenFnT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hdmWxbnH0ZYwh\m:{ZYPrbY4h\mmkcn;icIF{fHNiY3XscJMtKEmFNUCgQUAxNjJizszNMi=>	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
HeLa	M4\IPGZ2dmO2aX;uJIF{e2G7				MoDKTY5pcWKrdHnvckBw\iCKRFHDJIZzd21iaIXtZY4hUGWOYTDj[YxteyxiSVO1NEA:KDBwMzFOwG0v	NHr4fHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
MM96L/NFF	MVrGeY5kfGmxbjDhd5NigQ>?				MX\T[Yxm[3Srdnn0fUBqdmSneDygdoF1cW9ib3[gTWM2OCCob4KgbJVu[W5iTV25Omwh[2WubIOgeI8hUUN3MDDmc5IhcHWvYX6gUmZHKGOnbHzzMEBKSzVyIE2gOk44KM7:TT6=	M4WyflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
293T	MlTaSpVv[3Srb36gZZN{[Xl?				MWHJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNzIHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yM{[2JO69VS5?	MlX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OUe4NlYoRjF6M{m3PFI3RC:jPh?=
293T	NVSydWtGTnWwY4Tpc44h[XO\|YYm=				MlHQTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFNDDlfJBz\XO\|ZXSgbY4hOjl\|VDDj[YxteyxiSVO1NEA:KDBwME[yPUDPxE1w	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN7N{iyOkc,OTh\|OUe4NlY9N2F-
293T	M3nnZmZ2dmO2aX;uJIF{e2G7				M3frSGlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{[g[ZhxemW\|c3XkJIlvKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkC4N\|Mh\|ryPLh?=	NH:3O3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5O\|gzPid-MUizPVc5OjZ:L3G+
HeLa	NF7DPItHfW6ldHnvckBie3OjeR?=				NVG3Wnc2UW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDJIlvKEinTHGgZ4VtdHNiYomg[oxwfXJiZHWgcJl{KGG\|c3H5MEBKSzVyIE2gNE4xODVizszNMi=>	NXTVcXU2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPVc5OjdpPkG4N\|k4QDJ5PD;hQi=>
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insect cell	MkPHSpVv[3Srb36gZZN{[Xl?		MV22NEBucW6\|		M{HHTmlvcGmkaYTpc44hd2ZiZoXscEBt\W6pdHigdoVkd22kaX7hcpQhcHWvYX6gR{11\XKvaX7hcEBJcXNvdHHn[4VlKEiGQVOzM24ufGW{bXnuZYwhT1OWLYTh[4dm\CC{ZXPvcYJqdmGwdDDoeY1idiCQQ1;SNkApOzl3IITvJFQ5QSC{ZYPp[JVmeyliZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{NWmwZnXjeIVlKGmwc3XjeEBk\WyuczD1d4lv\yCUSFvLLGFkMUGPQzDhd{B{fWK\|dILheIUh[W[2ZYKgOlAhdWmwczDifUBndHWxcnnt[ZRmeixiS3mgQUAxNjByMEi0JO69VS5?	NXnwdIFwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
insect cell	MV3GeY5kfGmxbjDhd5NigQ>?		MmLWOlAhdWmwcx?=		MX3Jcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIILlZ49u[mmwYX70JIh2dWGwIFOteIVzdWmwYXygS3NVNXSjZ3fl[EBJTEGFMjDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCjZoTldkA3OCCvaX7zJIJ6KG[udX;ybY1mfGW{LDDLbUA:KDBwMECzOVUh\|ryPLh?=	NVn6Wmw6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
insect cell	M1LYTGZ2dmO2aX;uJIF{e2G7				NYL5SnBsUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDISGFEOTBiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{NWmwZnXjeIVlKGmwc3XjeEBk\WyuczD1d4lv\yCobIXvdo9o\W6rYzDw[ZB1cWSnIGLIT2tC[yCjczDzeYJ{fHKjdHWgZpkh\my3b4LpcYV1\XJuIFvpJF0hOC5yMES3OkDPxE1w	NHHV[VY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
insect cell	MnfaSpVv[3Srb36gZZN{[Xl?		MXG2NEBucW6\|		MnPyTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCFLYTldo1qdmGuIFjpd{11[WepZXSgTGRCSzhiKEGgeI8hOzd5IILld4llfWW\|KTDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT{hC[ymNKFHjLWFOSyCjczDzeYJ{fHKjdHWgZYZ1\XJiNkCgcYlveyCkeTDmcJVwemmvZYTldkwhU2liPTCwMlE1PSEQvF2u	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6OUS0NUc,Ojl3OEm0OFE9N2F-
insect cell	MUPGeY5kfGmxbjDhd5NigQ>?				M13BSWlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iTj30[ZJucW6jbDDIbZMufGGpZ3XkJGhFSUNzMTCoNUB1dyB\|NEegdoV{cWS3ZYOpJIV5eHKnc4Pl[EBqdiCkYXP1cI93cXK3cz3pcoZm[3SnZDDpcpNm[3RiY3XscJMhfXOrbnegVmhMUyiDYznBUWMh[XNic4Xid5Rz[XSnIHL5JIZtfW:{aX3leIVzNCCNaTC9JFAvPDBzIN88UU4>	MmPYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
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HEK293	MX3GeY5kfGmxbjDhd5NigQ>?	MVq2NFAhdk1?			MYjJcoNz\WG\|ZTDpckBxem:2ZXnuJIRqe3WuZnnk[UBqe2:vZYLhd4UhdGW4ZXygbY4hUEWNMkmzJINmdGy\|IHH0JFYxOCCwTTDifUBqdW23bn;icI91	NVr1TpV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1OlY4OzJpPkG3OVY3PzN{PD;hQi=>
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HCT116	NHX2eJJHfW6ldHnvckBie3OjeR?=				M1jsPGlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{GgZZN{\XO\|ZXSgZZMhcGm\|dH;u[UBpgXCncnHj[ZR6dGG2aX;uJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCkeTDX[ZN1\XKwIHLsc5Q>	M3XETVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MEW0NlM6Lz5zOEC1OFI{QTxxYU6=
HCT116	MnGwSpVv[3Srb36gZZN{[Xl?				MX\Jcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN4IHHzd4V{e2WmIHHzJJR2[nWuaX6gbJlx\XKjY3X0fYxifGmxbjDpckBpfW2jbjDIR3QyOTZiY3XscJMh[nliV3XzeIVzdiCkbH;0	Mni0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNUSyN\|koRjF6MEW0NlM6RC:jPh?=
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NCI-H661	NG\SRnNHfW6ldHnvckBie3OjeR?=	MnrLN\|AxKG6P	NUG1TFdCPiCqcoO=		M1TIWGlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBPS0lvSE[2NUBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiaHnzeI9v\SCKNDDhZ4V1gWyjdHnvckBifCB\|MECgcm0h[W[2ZYKgOkBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{	Mo\sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl\|OEW2NFAoRjF7M{i1OlAxRC:jPh?=
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primary bronchial epithelial cell	NVG5VHRQTnWwY4Tpc44h[XO\|YYm=	MnHkNE4yKHWP			NGnpeJZKdmirYnn0bY9vKG:oIFjERWM4KGmwIHj1cYFvKHC{aX3hdpkh[nKxbnPobYFtKGWyaYTo[Yxq[WxiY3XscJMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJI12fGGwdDDG[IVtfGF3MEigR2ZVWiCycn;0[YlvKGGyaXPhcEB{fXKoYXPlJIxw[2GuaYrheIlwdiCjdDCwMlEhfU1?	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4Nke4PUc,OTl7Nk[3PFk9N2F-
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MCF7	NHHUU5pHfW6ldHnvckBie3OjeR?=	NF3VVm8xNjVidV2=	NFTPT3QzPCCqcoO=		NYTsdIhZUW6qaXLpeIlwdiCxZjDDcIF{eyBzIHjpd5RwdmViZHXhZ4V1gWyjc3WgbY4hcHWvYX6gUWNHPyClZXzsd{Bie3Onc4Pl[EBieyC3cD3y[Yd2dGG2aX;uJI9nKHB{MTDwdo91\WmwIHzleoVtKGG2IECuOUB2VSCjZoTldkAzPCCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|	Mn\oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OECzOlMoRjJ{MkiwN\|Y{RC:jPh?=
MCF7	M37jZWZ2dmO2aX;uJIF{e2G7	Ml\UNE42KHWP	MoDaNlQhcHK\|		M120RWlvcGmkaYTpc44hd2ZiSFTBR\|YhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDjbIFv\2ViaX6geJVjfWyrbjDhZ4V1gWyjdHnvckBt\X[nbDDheEAxNjVidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	Mm\RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OECzOlMoRjJ{MkiwN\|Y{RC:jPh?=
MCF7	MUjGeY5kfGmxbjDhd5NigQ>?	Ml;BNE42KHWP	NWPib5lwOjRiaILz		NFLuOlZKdmirYnn0bY9vKG:oIFjERWMyKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgeZAuemWpdXzheIlwdiCxZjDhZ4V1gWyjdHXkJIhqe3SxbnWgTFMheHKxdHXpckBt\X[nbDDheEAxNjVidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	NGjBU\|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK4NFM3Oyd-MkKyPFA{PjN:L3G+
PC12	NHj0U3ZHfW6ldHnvckBie3OjeR?=	MoDWNVAhfU1?	NE\yWpc1QCCqcoO=		MYfJcohq[mm2aX;uJI9nKEiGQVO2JIlvKHKjdDDQR\|EzKGOnbHzzJIF{e2W\|c3XkJIF{KGGlZYT5cIF1cW:wIH;mJIhqe3SxbnWgTFMh[XRiMUCgeW0h[W[2ZYKgOFghcHK\|IHL5JHdme3Sncn6gZoxwfHSrbnegZY5idHm\|aYO=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyNU[4NEc,OjN7MEW2PFA9N2F-
PC12	M2rweGZ2dmO2aX;uJIF{e2G7	MX6xNEB2VQ>?	M3PtTVQ5KGi{cx?=		MojETY5pcWKrdHnvckBw\iCKRFHDOkBqdiC{YYSgVGMyOiClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gZYxxcGFvdIXieYxqdiCjY3X0fYxifGmxbjDheEAyOCC3TTDh[pRmeiB2ODDodpMh[nliV3XzeIVzdiCkbH;0eIlv\yCjbnHsfZNqew>?	NELvRWo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
PC12	MUfGeY5kfGmxbjDhd5NigQ>?	M3nJdlExKHWP	M{\zbFUh\GG7cx?=		MWDJcoR2[3Srb36gc4YhdmW3cnn0[UBwfXSpcn;3eIghcW5icnH0JHBEOTJiY3XscJMh[XRiMUCgeW0h[W[2ZYKgOUBl[Xm\|	NYniXVJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
SH-SY5Y	MVjGeY5kfGmxbjDhd5NigQ>?	MmW1NVAhfU1?	NFjHOXI2KGSjeYO=		MYrJcoR2[3Srb36gc4YhdmW3cnn0[UBwfXSpcn;3eIghcW5iaIXtZY4hW0hvU2m1XUBk\WyuczDheEAyOCC3TTDh[pRmeiB3IHThfZM>	NH7URXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
A549	NWPKdm12SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=	MXqwMlQhfU1?	M3\UbFI1KGi{cx?=		MYDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgd4hzfW6tZX6gZY5lKHOqYYLwJJNp[XCnIHH0JFAvPCC3TTDh[pRmeiB{NDDodpMh[nlicHjhd4Uh[2:wdILhd5QhdWmlcn;zZ49xgQ>?	NFrqelU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4OFEyOid-MkW4PFQyOTJ:L3G+
HeLa	M1[xSGZ2dmO2aX;uJIF{e2G7				MlnBTY5pcWKrdHnvckBw\iCKRFHDOkBqdiCqdX3hckBJ\UyjIHPlcIx{KGG\|c3Xzd4VlKGG\|IIT1ZpVtcW5iYXPleJlt[XSrb36gbY5kfWKjdHXkJIZweiCxdnXycolocHRiYomgbY1ufW6xZnz1c5Jme2OnbnPlJI1q[3Kxc3PvdIlkKGGwYXz5d4l{	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZzMUmxPUc,OjZ4MUG5NVk9N2F-
C127-LT	NVP6OGFiTnWwY4Tpc44h[XO\|YYm=	NF\IfmYzODBibl2=	MVOyOEBpenN?		NFjrV4xKdmirYnn0bY9vKG:oIFjERWMhcW5ibX;1d4UhSzF{Nz3MWEBk\WyuczDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIFXHSnAh\XiycnXzd4lwdiCjdDCyNFAhdk1iYX\0[ZIhOjRiaILzJIJ6KG[udX;y[ZNk\W6lZTDtbYNzd3Olb4D5	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl7NkO3Nkc,OjZ7OU[zO\|I9N2F-
HeLa nuclear	M2DwXWZ2dmO2aX;uJIF{e2G7	MX[xNEB1dyB{NTD1US=>	M1XW[VMxKG2rboO=		NVziWmxGS2:vcHX0bZRqfmViaX7obYJqfGmxbjDv[kBJTEGFIHnuJIh2dWGwIFjlUIEhdnWlbHXhdkBk\WyuczDheEAyOCC2bzCyOUB2VSC3c3nu[{BDd2NvYXPleJltNWy7c3nu[U1CVUNiYYOgd5Vje3S{YYTlJIFnfGW{IEOwJI1qdnNiYomgUIlv\XenYY\ldk1DfXKtIIDsc5Qh[W6jbInzbZM>	NXTlPYQ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVY{PzJpPkK2PVk3Ozd{PD;hQi=>
HeLa	MWDGeY5kfGmxbjDhd5NigQ>?	NHv2UpczKHWP	MljoNlQhcHK\|		MnHVTY5pcWKrdHnvckBw\iCKRFHDNUBqdiCqdX3hckBJ\UyjIHPlcIx{KGG\|c3Xzd4VlKGG\|IHj5dIVz[WOndInsZZRqd25ib3[gbIl{fG:wZTDIN{BifCB{IIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	NI[4Z4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
HN13	MY\GeY5kfGmxbjDhd5NigQ>?		NHXRT3czPCCqcoO=		MofiTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhPOTNiY3XscJMh[XO\|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIhqe3SxbnWgTFMhVHm\|OTDhZ4V1gWyjdHnvckBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDpcY12dm:obIXvdoV{[2WwY3WgZZN{[Xl?	NYjVfG4yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwN\|ExPjZpPkK5NFMyODZ4PD;hQi=>
HeLaS3	NGTmboxHfW6ldHnvckBie3OjeR?=	NVi5XXFCOTBidV2=			NFT2Rm9KdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYWOzJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGirc4TvcoUhUDONOTDhZ4V1gWyjdHnvckBifCBzMDD1UUBu\WG\|dYLl[EBqdW2nZHnheIVtgSCkeTDpcY12dm:kbH;0JIFv[Wy7c3nz	Moi5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NUi1N\|UoRjNyMkW4OVM2RC:jPh?=
HeLaS3	NUXwPWlrTnWwY4Tpc44h[XO\|YYm=	NYTRU\|ltOTBidV2=	MkX3NVYhcHK\|		NVLkdm1PUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHHTN{Bk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCqaYP0c45mKEh\|S{mgZYNmfHmuYYTpc44h[XRiMUCgeW0hfHKnYYTl[EBnd3JiMU[gbJJ{KG[xbHzve4VlKGK7IHPvcZBwfW6mIIfhd4hwfXRiYX7kJI1m[XO3cnXkJJVxKHSxIEK0JIhzeyCkeTDpcY12dm:kbH;0JIFv[Wy7c3nz	NHj3cnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEK1PFU{PSd-M{CyOVg2OzV:L3G+

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	EGFR / STAT3 ; PubMed: 31289542 Oncol Lett Expression of EGFR and STAT3 in PC3 cells treated with TSA. PC3 cells were plated in 6-cm dishes, and 0.25, 0.5 or 1 µM TSA was added for 24 h. DMSO was added into the control group wells. Cells were collected and lysed with lysis buffer and the protein expression analyzed via western blotting. Cyclin D1 / CDK6 / CDK4 / p-RB / RB; PubMed: 31289542 Oncol Lett Expression of cyclin D1, CDK6, CDK4 and RB, and the phosphorylation of RB, was detected in PC3 cells treated with TSA for 24 h. MMP-1 / MMP-3 / MMP-13 / TIMP-1; PubMed: 27431944 Mol Med Rep Chondrocytes were pretreated with increasing concentrations of TSA for 2 h, followed by stimulation with 5 ng/ml IL-1β for 24 h. Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1); PubMed: 27431944 Mol Med Rep Chondrocytes were treated with increasing concentrations of TSA for 24 h. α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2; PubMed: 23802098 Front Oncol Histone acetylation occurs directly at the promoter region of IL-23R. The ChIP assay demonstrates that TSA treatment results in an increase in the acetylation of histone H3 and H4. A549 cells were cultured in the presence or absence of TSA (250 ng/mL) for a period of 24 h. Subsequently, a ChIP assay was performed using the following antibodies; pan acetyl-histone H3 (H3Ac), pan acetyl-histone H4 (H4Ac), acetyl-histone H3 Lys 9 (H3K9Ac), acetyl-histone H3 Lys 9/14 (H3K9/14ac), acetyl-histone H3 Lys 9 phosphoSer10 (H3K9S10), methyl-histone H3 Lys 4 (H3K4Me), di methyl-histone H3 Lys 4 (H3K4Me2), and di methyl-histone H3 Lys 9 (H3K9Me2). Input DNA serves as a positive control as recommended by the manufacturer (Diagenode). A no antibody control was included to test for non-specific binding (UT, untreated; TSA, Trichostatin A).	31289542 27431944 23802098
Immunofluorescence	α-C/EBPβ / α-HP1α ; PubMed: 21122806 Exp Cell Res 3T3-L1 preadipocytes were grown on coverslips for three days and induced to differentiate by treatment with MDI (48 h) in the presence of vehicle (-TSA) or 87 nM Trichostatin A (+TSA). Under these experimental conditions: A-H Cells were fixed and permeabilized in cold methanol, subjected to IIF with the indicated antibodies, and nuclei were counterstained with DAPI. Scale bar, 5 μm. α-HDAC1 ; PubMed: 29045501 PLoS One MCF-7 cells were treated with indicated concentrations of trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels. α-tubulin / acetylated histone 3; PubMed: 27957719 Neurotherapeutics Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775.	21122806 29045501 27957719
Growth inhibition assay	Cell viability (A549 cells); PubMed: 27571418 PLoS One Cytotoxicity of histamine determined by MTT assay in A549 cells. Cell viability (MDA-MB-231 cells); PubMed: 27548148 Int J Mol Sci The human breast cancer cells (MDA-MB-231) were incubated with various concentrations of TSA (0–300 nM) for 24 h. Cell viability was measured by WST-8. Cell viability (PC3 cells); PubMed: 31289542 Oncol Lett PC3 cells were plated in a 96-well plate and treated with different doses of TSA (0, 0.25, 0.5 and 1 µM) for 72 h; an MTT assay was used to measure the effects of TSA on PC3 cell proliferation.	27571418 27548148 31289542

体内研究

Administration of Trichostatin A 按 0.5 mg/kg 剂量处理N-甲基-N-亚硝基脲致癌物诱发的大鼠乳腺癌模型，持续处理4周，具有有效的抗癌活性，即使按按高达5 mg/kg剂量处理也没有任何可测量到的毒性。^[1]Trichostatin A 按10 mg/kg 剂量单独腹腔注射给药非转基因和脊髓性肌萎缩症（SMA）模型鼠，导致乙酰化的 H3 和 H4组蛋白水平提高，也导致活运动神经元(SMN) 基因表达稍微提高。Trichostatin A 每天按10 mg/kg剂量处理SMA模型鼠，促进存活, 减轻体重下降,且增强运动行为。^[5]

激酶实验：^[1]	- 合并体外HDAC活性 : 从每种乳腺癌细胞系(MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51,或 SK-BR-3)中准备全部细胞提取物。在溶于0.1% (v/v)乙醇的不同浓度Trichostatin A存在时，或者使用0.1% (v/v)乙醇作为对照, 20 μL 细胞粗提取物(~2.5 ×10⁵ 个细胞)与 1 μL ~1.5 × 10⁶ cpm [³H]乙酰标记的组蛋白 H4 肽底物(第2-20位NH2-末端残基) 在25^oC下温育60分钟。使用50 μL 1 M HCl/0.16 M 乙酸对每组 200-μL 反应进行淬火，然后使用600 μL 乙酸乙酯进行抽提，通过闪烁计数器测量释放的[³H]乙酸。使用非线性回归曲线拟合抑制数据，绘制合适的剂量反应曲线，而测定IC50。
细胞实验：^[1]	- 合并 Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3 Concentrations: 溶于无水酒精,终浓度为~10 μM Incubation Time: 96小时 Method: 使用不同浓度Trichostatin A处理细胞96小时。处理后,使用sulforhodamine B 比色分析测定细胞增殖。通过台酚蓝染色排除法测定细胞活力。 (Only for Reference)
动物实验：^[1]	- 合并 Animal Models: 携带NMU诱导的肿瘤的近交系(Ludwig/Wistar/Olac)处女鼠 Dosages: ~5 mg/kg/day Administration: 皮下注射 (Only for Reference)

参考文献

- 合并

溶解度 (25°C)

体外	DMSO	23 mg/mL (76.05 mM)
	Water	Insoluble
	Ethanol	Insoluble
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+2% Tween 80+ddH2O	1.5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Trichostatin A (TSA) SDF

分子量	302.4
化学式	C₁₇H₂₂N₂O₃
CAS号	58880-19-6
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	N/A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-05-17)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03887650	Unknown status	Drug: Liposomal Bupivicaine 1.3%\|Drug: Bupivacaine 0.5%	Post-operative Pain\|Total Shoulder Arthroplasty\|Osteoarthritis of the Shoulder\|Pain Management	Hartford Hospital	January 14 2019	Phase 4
NCT03838926	Unknown status	Drug: Trichostatin A	Relapsed or Refractory Hematologic Malignancies	Vanda Pharmaceuticals	September 27 2018	Phase 1
NCT02976272	Unknown status	Other: bortezomib	Multiple Myeloma\|Bortezomib Regimen\|Adult	University Hospital Clermont-Ferrand\|Laboratoires Takeda	June 7 2016	--

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?
回答：
Our S1045 Trichostatin A (TSA) is R enantiomer.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

选择性强的HDAC抑制剂

Rocilinostat (ACY-1215) : HDAC5-selective, IC50=5 nM.
活性最强的HDAC抑制剂

Quisinostat (JNJ-26481585) : HDAC1, IC50=0.11 nM; HDAC2, IC50=0.33 nM.
FDA批准的HDAC抑制剂

Vorinostat (SAHA, MK0683) : Approved by FDA against cutaneous T cell lymphoma.
最新的HDAC抑制剂

RG2833 (RGFP109) ^New : Brain-penetrant HDAC inhibitor with IC50 of 60 nM and 50 nM for HDAC1 and HDAC3, respectively.

研究领域

产品类型

定制您的化合物库

Trichostatin A (TSA)

客户使用Selleck生产的Trichostatin A (TSA)发表文献185篇：

产品安全说明书

HDAC抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Trichostatin A (TSA) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-05-17)

技术支持

常见问题及建议解决方法

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

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