Trichostatin A (TSA)

For research use only. Not for use in humans.

目录号：S1045 中文名称：曲古柳菌素A

CAS No. 58880-19-6

Trichostatin A (TSA)是一种HDAC抑制剂，无细胞试验中IC50为1.8 nM左右。

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客户使用Selleck生产的Trichostatin A (TSA)发表文献211篇：

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产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述

Trichostatin A (TSA)是一种HDAC抑制剂，无细胞试验中IC50为1.8 nM左右。

靶点

HDAC ^[1]
(Cell-free assay)

~1.8 nM

体外研究

Trichostatin A 抑制八种乳腺癌细胞系，包括MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3增殖，平均IC50为124.4 nM (范围为26.4-308.1 nM),作用于表达ERα的细胞系比作用于表达 ERα阴性的细胞系更有效。Trichostatin A作用于全部乳腺癌细胞系，抑制HDAC活性，平均IC50为 2.4 nM (范围为0.6-2.6 nM), 产生显著的组蛋白 H4高度乙酰化。^[1]与 Trapoxin (TPX) 和 Chlamydocin 有效抑制HDAC1 或 HDAC4而不是 HDAC6不同，Trichostatin A 抑制这些HDACs，且抑制程度相似， IC50 分别为6 nM, 38 nM, 和8.6 nM。^[2] Trichostatin A (100 ng/mL) 作用于MIA PaCa-2细胞，通过招募p300和 PCAF进入Sp1-NF-Y HDAC复合体，而诱导转化生长因子βⅡ型受体(TβRII) 表达，复合体与TβRII 启动子的DNA片段结合，伴随着Sp1乙酰化，且与复合体相关的HDAC数理全部降低。^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
HeLa	MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MlvZNVAwOzBxNUCgcm0>	M4Dob\|czKGh?	M{LudGROW09?	M2X5TIlv[3KnYYPld{B1cGViboXtZoVzKG:oIF3NVEAp\|pUQqH2pJIxwe3NiY3XscJMh\G:\|ZTDk[ZBmdmSnboTsfS=>	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF4NUe0PEc,OjNzNkW3OFg9N2F-
HeLa	M2\ZeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYiyNEBvVQ>?	MY[3NkBp	MmW1SG1UVw>?	MYHJR\|UxKG:oIEKwcm0>	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF4NUe0PEc,OjNzNkW3OFg9N2F-
HeLa	NEPWbHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NGPDbVY1OCCwTR?=	M3vBXlQ5KGh?	MoTHSG1UVw>?	NG\UOFZKSzVyIH;mJFQxdk1?	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF4NUe0PEc,OjNzNkW3OFg9N2F-
HeLa	M{\YN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3jtOFExOCCwTR?=	NIXxRY0zPCCq	NX62OXJuTE2VTx?=	M{jQdGlEPTBib3[gNVAxdk1?	M1flR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MU[1O\|Q5Lz5{M{G2OVc1QDxxYU6=
Caco-2	MUDGeY5kfGmxbjDBd5NigQ>?	NYS2fVROOSEEtV5CpC=>	M4TUUlI1KGh?		M{LSPIRm[3KnYYPld{BUTVKWIIDyc5RmcW5iZYjwdoV{e2mxbh?=	NYHofoJuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxPVUxPzBpPkKzNVk2ODdyPD;hQi=>
HTK	M3\mb2Z2dmO2aX;uJGF{e2G7	NYHjcGZiOTByLUiwNI5O	MYK3NkBp		NGHSdoxjdG:la4OgWGdHNc7{4pETTY5lfWOnZDDSU3Mh[W6mIFiyU\|LDqEGlY4XteYxifGmxbh?=	M4jNVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|Mki0NFAzLz5{M{K4OFAxOjxxYU6=
HTK	NV;CZ\|hLTnWwY4Tpc44hSXO\|YYm=	Mlv4OFAxKG6P	NYm4XJV4PzJiaB?=		MVHpcohq[mm2czDUS2Yu\|rMkgKPJcoR2[2WmIF35c4Zq[nKxYnzhd5QhTGmoZnXy[Y51cWG2aX;u	Mn\KQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OESwNFIoRjJ\|Mki0NFAzRC:jPh?=
HEK293	MmHOSpVv[3Srb36gRZN{[Xl?	NYfofZlnOSEEtV5CpC=>	MUGxPEBpyqB?		NX71[JJpcW6qaXLpeJMhfGinIHvhcIlzcW5vNz3t[YRq[XSnZDDy[YNzfWm2bXXueEBw\iC\|eX7wbIltcW5vMTDh[4dz\WejdHXzJIlvfG9iYXfndoV{d22ncx?=	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ6NEi0PEc,OjN{OES4OFg9N2F-
SH-SY5Y	MXnGeY5kfGmxbjDBd5NigQ>?	NGHycZYzPTBibl2=	NGDJXJUyPiCq		MlXPZ4hidmenczDlfJBz\XO\|aX;uJJBifHSncn6gc4Yh\2WwZYOgbY53d2y4ZXSgbY4h[2ixbHXzeIVzd2xic4nueIhme2m\|LDD1dJRic2ViYX7kJIVn\my3eB?=	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzN{NkSyNkc,OjN\|Mk[0NlI9N2F-
THP1	Mm\PRZBweHSxc3nzJGF{e2G7	MlTjNUDPxE1?	Ml64NlQhcA>?		NVnH[lk1cW6mdXPld:Kh[2WubDDk[YF1cA>?	M{jGb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NECwOVE6Lz5{M{SwNFUyQTxxYU6=
K562	MoDjRZBweHSxc3nzJGF{e2G7	NUmyNVdkOSEQvF2=	MmPFNlQhcA>?		NHj3e45qdmS3Y3XzxsBk\WyuIHTlZZRp	NI\oVVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SwNFUyQSd-MkO0NFA2OTl:L3G+
Kazumi	M1\UXmFxd3C2b4Ppd{BCe3OjeR?=	NUPOVpd2OSEQvF2=	NVT5dppROjRiaB?=		M132UIlv\HWlZYRCpINmdGxiZHXheIg>	M1;JflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NECwOVE6Lz5{M{SwNFUyQTxxYU6=
KG1	MlTmRZBweHSxc3nzJGF{e2G7	M3z1W\|Eh\|ryP	MXGyOEBp		MUDpcoR2[2W\|wrDj[YxtKGSnYYTo	NXTp[oFHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
HL60	Mn\QRZBweHSxc3nzJGF{e2G7	MkPVNUDPxE1?	M4ryWVI1KGh?		NWDHZoN{cW6mdXPld:Kh[2WubDDk[YF1cA>?	M4rjWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NECwOVE6Lz5{M{SwNFUyQTxxYU6=
HEL	M1:1OmZ2dmO2aX;uJGF{e2G7	MnW2NE42KM7:TR?=	NGjJeJMzPCCq	MVPleIhidm:u	NYXrcHZU\GWlcnXhd4V{KHSqZTDlcpp6dWViYXP0bZZqfHlib3[gTGRCS3NiY3:teJJm[XSvZX70JJdqfGhiY4XyZ5VucW5?	M1\1b\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEOwPVU4Lz5{M{SzNFk2PzxxYU6=
K562	MoDzSpVv[3Srb36gRZN{[Xl?	NGPEWpQxNjVizszN	NFzjb24zPCCq	NFXmSm5mfGijbn;s	M4DoVoRm[3KnYYPld{B1cGViZX76fY1mKGGldHn2bZR6KG:oIFjERWN{KGOxLYTy[YF1dWWwdDD3bZRpKGO3cnP1cYlv	M33DUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEOwPVU4Lz5{M{SzNFk2PzxxYU6=
LM8	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MUWwMlMh\|ryP	NV3nOlRrOTJvOU[gbC=>	NVr2e4RsTE2VTx?=	MkTwbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHPvMZRz\WG2bXXueEB4cXSqIH3leIZwem2rbh?=	NYfrWIp7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVE5OTdpPkKzOFUyQDF5PD;hQi=>
MG-63	M1H3PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3HNO\|AvOyEQvF2=	NILTW3cyOi17NjDo	Ml;ISG1UVw>?	NEnjbmFqdmirYnn0d{B1cGViY3XscEBoem:5dHigZ48ufHKnYYTt[Y51KHerdHigcYV1\m:{bXnu	M4HBU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEWxPFE4Lz5{M{S1NVgyPzxxYU6=
H1299	MlH3SpVv[3Srb36gRZN{[Xl?	MnP6NE42KM7:TR?=	M3zkT\|Q5KGh?	M{HyTGROW09?	MojkbY5pcWKrdIOgeIhmKGmwdnHzbZZmdmW\|c9MgJINwdWKrbnWge4l1cCC\|aXzpZolvcW5?	NF7WVYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S2NVk4PSd-MkO0OlE6PzV:L3G+
H1299	NG\selVHfW6ldHnvckBCe3OjeR?=	NGLaNZIxNjVizszN	Ml3kOFghcA>?	MVTEUXNQ	NYnXZ\|EycW6qaXLpeJMhfGinIH3p[5JifG:{eTDwc5RmdnSrYXygZ49u[mmwZTD3bZRpKHOrbHnibY5qdg>?	MkfTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NkG5O\|UoRjJ\|NE[xPVc2RC:jPh?=
H1299	M1X2NWZ2dmO2aX;uJGF{e2G7	NYDHZZFMOC5\|M,MAl\|HDqML3TR?=	NGW0XHczPC92ODDo	MX\EUXNQ	MYTpcoNz\WG\|ZYOgSU1k[WSqZYLpckBxem:2ZXnuJIxmfmWuczDkc5NmKGSncHXu[IVvfGy7	NHL4[po9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S2NVk4PSd-MkO0OlE6PzV:L3G+
MCF7	M1jCOWZ2dmO2aX;uJGF{e2G7	MWSwMlA2NTJizszN	NUjJTHNuOjRiaB?=		NYn2bZJremWmdXPld{B1cGViaX7keYN1cW:wIH;mJJA2Oy2mZYDlcoRmdnRiZ3Xu[ZMh[nliToX0cIlvNTN?	MmfkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2N{C1OFAoRjJ\|NEewOVQxRC:jPh?=
ARN8	NYPTN5plTnWwY4Tpc44hSXO\|YYm=	Mnu2NE4xPS1{IN88US=>	MoSwNlQhcA>?		MUfy[YR2[2W\|IITo[UBqdmS3Y4Tpc44hd2ZicEWzMYRmeGWwZHXueEBo\W6nczDifUBPfXSuaX6tNy=>	NVLBTFA4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0O\|A2PDBpPkKzOFcxPTRyPD;hQi=>
U373	NX\uS3ZZTnWwY4Tpc44hSXO\|YYm=	MUGwMlI2NzBwNT:xJO69VQ>?	NVrpOGZNOjRiaB?=		M{DwT4lv[3KnYYPld{BRWi2EIHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOWF7[WSF	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR5NEG3NUc,OjN2N{SxO\|E9N2F-
HASMCs	NU\XZ2pITnWwY4Tpc44hSXO\|YYm=	NF7PWJYxNTVyMDDuUS=>	NXzjeIVZPiCm		MUXkc5dvNXKnZ4XsZZRmeyC2aHWg[ZhxemW\|c3nvckBw\iCKQVTDJIFv\CClZXzsJJZq[WKrbHn0fS=>	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzVzOES2O{c,OjN3MUi0Olc9N2F-
MFE296	NYXNS5d6TnWwY4Tpc44hSXO\|YYm=	NV74PHA3PTByIH7N	NWG1UIF7PSCm	NGOwOGdFVVOR	Mo[3bY5lfWOnczDMNWNCVcLiZYjwdoV{e2mxbjDjc{11emWjdH3lcpQhf2m2aDC1MWF7[UN?	NIXOWoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNFc3QSd-MkO1N\|A4Pjl:L3G+
EN1	NH;o[I5HfW6ldHnvckBCe3OjeR?=	NILDdYs2ODBibl2=	M{D0d\|Uh\A>?	M3vEV2ROW09?	MkLWbY5lfWOnczDMNWNCVcLiZYjwdoV{e2mxbjDjc{11emWjdH3lcpQhf2m2aDC1MWF7[UN?	NYTBTZdIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1N\|A4PjlpPkKzOVMxPzZ7PD;hQi=>
HEC1A	NXPVbHBHTnWwY4Tpc44hSXO\|YYm=	MUm1NFAhdk1?	MUG1JIQ>	M1TW[WROW09?	M1XEc4lv\HWlZYOgUFFESU4EoHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOU1CgmGF	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV\|MEe2PUc,OjN3M{C3Olk9N2F-
ECC1	MV\GeY5kfGmxbjDBd5NigQ>?	NVO1eWZ6PTByIH7N	Mnv6OUBl	MVLEUXNQ	NXy2cpFicW6mdXPld{BNOUODTdMg[ZhxemW\|c3nvckBkdy22cnXheI1mdnRid3n0bEA2NUG8YVO=	NGTPW4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNFc3QSd-MkO1N\|A4Pjl:L3G+
AGS	M32xXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NX3UNpFbOC5yMUWtNUDPxE1?	MV[3NkBp		NGTGUXdqdmS3Y3XzJINmdGxiZHXheIgh[2:wY3XueJJifGmxbjDk[ZBmdmSnboTsfS=>	NV6xcVBZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3OFUxOjRpPkKzO\|Q2ODJ2PD;hQi=>
Huh7	NV3tNXlRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NXXQcpp[OSEQvF2=	MWmyOEBp	M4fvb2ROW09?	MVHy[YR2[2W\|IHHic5V1KDJyJTD2bYFjcWyrdIpCpC=>	NXW5NXczRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFM6OzNpPkKzOlQ{QTN\|PD;hQi=>
Lovo	MnSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MnnzNE4zNzFwMD:1MlAh\|ryP	NYS3cnRkOTJxMkSvN\|YwPDhiaB?=		MoK2bY5lfWOnczDj[YxtKGSnYYToJIlvKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK=	NELqflU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e3NFAxOCd-MkO3O\|AxODB:L3G+
HCT1	NVfrZ\|dnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MkiyNE4zNzFwMD:1MlAh\|ryP	M2HkbVEzNzJ2L{O2M\|Q5KGh?		NX2xV2QzcW6mdXPld{Bk\WyuIHTlZZRpKGmwIHPvcoNmdnS{YYTpc44uKGGwZDD0bY1mNWSncHXu[IVvfCCvYX7u[ZI>	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd5MECwNEc,OjN5N{CwNFA9N2F-
SUM190PT	M1vFRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NVTreGp1PTBxMUCwM\|I2OCCwTR?=	Mmr4OFghcA>?	NHr3dGFFVVOR	NVjFfmw{cW6mdXPld{Bk\WyuIHTlZZRpKGG2IEWwJI5OKHOnboPpeIl3\Wy7	NIjFSVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlY{QCd-MkO3PVI3Ozh:L3G+
SUM149PT	NV3COGg5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Mk\3Nk84NjVxMUCg{txO	MmDkOFghcA>?	NUHBRnd7TE2VTx?=	NYL1NVFjcW6mdXPld{Bk\WyuIHTlZZRpKGG2IEKg{txOKGmwc3Xud4l1cX[nbIm=	MlX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OUK2N\|goRjJ\|N{myOlM5RC:jPh?=
H1299	M2S0N2Fxd3C2b4Ppd{BCe3OjeR?=	NYnueWVCOC53L{Gg{txO	NUm3b5ppPDhiaB?=	NEfKRZpmfGijbn;s	NYjxd4kzcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?=	MomxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6Nke5PVEoRjJ\|OE[3PVkyRC:jPh?=
A549	NUDRcYdxSXCxcITvd4l{KEG\|c3H5	NHH5XHMxNjVxMTFOwG0>	NX2xdVlRPDhiaB?=	NGXMe2pmfGijbn;s	MX;pcoR2[2W\|IHPlcIwh[XCxcITvd4l{	Mn\RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6Nke5PVEoRjJ\|OE[3PVkyRC:jPh?=
H1299	NVznco1uT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NH\kbHQxNjJ3L{CuOU8yKM7:TR?=	MmHQNlQwPDhxN{KgbC=>	MXLleIhidm:u	MkLkbY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIHnuJINwdmOnboTyZZRqd25iYX7kJJRqdWVvZHXw[Y5l\W62IH3hco5meg>?	NVvqcoJuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4Olc6QTFpPkKzPFY4QTlzPD;hQi=>
A549	NEXYR\|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MX6wMlI2NzBwNT:xJO69VQ>?	MUiyOE81QC95MjDo	NXXTVFRP\XSqYX7vcC=>	MUXpcohq[mm2czD0bIUh[2WubDDndo94fGhiaX6gZ49v[2WwdILheIlwdiCjbnSgeIlu\S2mZYDlcoRmdnRibXHucoVz	M13sTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OE[3PVkyLz5{M{i2O\|k6OTxxYU6=
H1299	NXT6T3lYT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MnTFNeKh\|ryPwrC=	NGTRcGgzPC92OD:3NkBp	M2CwSoV1cGGwb3y=	MkHkbY5kemWjc3XzJINmdGxiZHXheIgh[W[2ZYKgNlRp	NECyT4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mxOlYxQSd-MkO5NVY3ODl:L3G+
PMNs	M3LtRWZ2dmO2aX;uJGF{e2G7	MYSzNEBvVQ>?	NEHFcJI1QCCq		NIHJZ3hqdmirYnn0d{Bm\m[ncn;jfZRwe2m\|LDDISGFEKGGldHn2bZR6KGGwZDDSZYMh[WO2aY\peJk>	M{PQRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUi4OlE4Lz5{M{m4PFYyPzxxYU6=
CA46	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M{XJZlMwPi9zMj:yOE81QCCwTR?=	NVvPTGtnPDhiaB?=		M{XEZYlvcGmkaYTzJJRp\SClZXzsJJBzd2yrZnXyZZRqd25iYYSgNlQh[W6mIES4JI5OyqB?	MkDSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyNkS5OVEoRjJ2ME[0PVUyRC:jPh?=
HCT116	NYjsO5NuT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2LB[\|AvOiEQvF2=	NYjIWIZpOTJiaB?=		Mlrp[Y5p[W6lZYOgZ4VtdCCjcH;weI9{cXNiaX7keYNm\CCkeTDyZYRq[XSrb36=	M1ewTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUKyNlMyLz5{NEGyNlI{OTxxYU6=
HLEB3	NIrZPFlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3PnOVAvPC9yLkig{txO	NX61R5hTPDhiaB?=	MVrEUXNQ	NFLTZmdqdmS3Y3XzJINmdGxiYYDvdJRwe2m\|	NUfRS2k{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
SRA01/04	NEP3SIdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MoP2NE41NzBwODFOwG0>	MkH5OFghcA>?	NUfESYtsTE2VTx?=	MUnpcoR2[2W\|IHPlcIwh[XCxcITvd4l{	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF3N{i3PEc,OjRzNUe4O\|g9N2F-
HLEB3	NF\NSHJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NES0UpMxNjJizszN	NFvkbGo1QCCq	M4fUSWROW09?	NGjUWIJ{fXCycnXzd4V{KGOnbHygdJJwdGmoZYLheIlwdiC2aILveYdpKHO3cIDy[ZN{cW6pIITo[UBRUTONL1HreEwheDN6TVHQT{BidmRiRWLLNU8zKHOrZ37hcIlv\yCyYYToe4F6ew>?	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF3N{i3PEc,OjRzNUe4O\|g9N2F-
SRA01/04	NETtclBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MWWwMlIh\|ryP	Mo[0OFghcA>?	M4rMdGROW09?	NUPMUYkye3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC\|dYDwdoV{e2mwZzD0bIUhWEl\|Sz;Bb5QtKHB\|OF3BVGsh[W6mIFXST\|EwOiC\|aXfuZYxqdmdicHH0bJdigXN?	NV3JflZXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
A2780	M17RfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MX2wMlA2NTJizszN	NFm5fXEyOC9{ND:0PEBp	MVHEUXNQ	M4fXWY1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{	M3TmZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MkKzPFAyLz5{NEKyN\|gxOTxxYU6=
SKOV3	NI\NbmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M2O0SFAvODVvMjFOwG0>	MV6xNE8zPC92ODDo	MnvwSG1UVw>?	M1z1do1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ{M{iwNUc,OjR{MkO4NFE9N2F-
Huh7	M3rCOWZ2dmO2aX;uJGF{e2G7	MnvrNE4yNzBwNT:xMlAh\|ryP	NWDIbIdHOjSq		MmfVd5VxeHKnc4Pld{B[XEiGQ{Kg[4Vv\SCneIDy[ZN{cW:w	MkX3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{Nkm2O\|IoRjJ2Mk[5OlczRC:jPh?=
hMSCs	M{fhOWZ2dmO2aX;uJGF{e2G7	M1HhVVYvOjVibl2=	MWOyOEBp	NHLHXm1FVVOR	M3O5OpN1[WKrbHn6[ZMhUGm\|dH;u[UBC[2W2eXzheIlwdiCjbnSgeIhmKEW6cILld5Nqd25ib3[gVIx2emmyb4TlcpQhT2WwZYO=	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNzMkO1Okc,OjR\|MUKzOVY9N2F-
HT29	NXzycnlZT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NH7YdVkyQDBibl2=	MWS3NkBp		M2PoOmlEPTBib3[gNVgxKG6P	Moq1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR\|NkiyOlUoRjJ2M{[4NlY2RC:jPh?=
RPE	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmDuNE4zNzBwND:wMlgwOSEQvF2=	MUSyOE81QC95MjDo	MUfEUXNQ	MYPpcohq[mm2czD0bIUheHKxbHnm[ZJifGmxbjDifUBk\WyuIHP5Z4xmKGG{cnXzeC=>	NYiydWd{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVY3ODJpPkK0OFU3PjB{PD;hQi=>
U87 GBM	M4rUZWZ2dmO2aX;uJGF{e2G7	M2\PSlExOC93MECgUo0>	NHHnTVU1QCCq	NE\NNFQyODBnIHX0bIFvd2x?	NEPUXWNKdmS3Y3XzJHNmdmW\|Y3XuZ4UuVGmtZTDBcJRmemG2aX;ud{BqdiCQdXPs[YFzKE2xcoDoc4xw\3l?	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR4NEi0NUc,OjR2NkS4OFE9N2F-
U87 GBM	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M2\0cFExOC9\|MECvOVAxKG6P	NWj6XG5yPzJiaB?=	MmrkNVAxLSCndHjhco9t	NUXUTJM4emWmdXPld{Bu\WGwIHPlcIwhdnWvYnXyJIJ6KDNzLDC1OEwh[W6mIEW4xsAm	M{niWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NE[0PFQyLz5{NES2OFg1OTxxYU6=
ACP03	MkP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHnQSHkzPTBxM{WwM\|UxOG6P	NIL3UoYzPGh?		NHXico1l\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgc4Yh[XCycn;4bY1ifGWueTC3NOKhLSCjdDCyOVDDqG6P	NYHNb5ZERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2Olg2PDdpPkK0OlY5PTR5PD;hQi=>
ACP02	NV:5PYg1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NVfKfol3OjVyL{O1NE82ODCwTR?=	M3X6XlI1cA>?		MW\k[YNz\WG\|ZYOgZ4VtdCC4aXHibYxqfHlib3[gZZBxem:6aX3heIVtgSB5MNMgKUBifCB{NUFCpI5O	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ4OEW0O{c,OjR4Nki1OFc9N2F-
HeLa	NIfaRW1HfW6ldHnvckBCe3OjeR?=	NYXrflhKOW2P	NHe4SIQxNjWq		NVuydVh4[WKxbHnzbIV{KHSqZTD0c5RidCCKRFHDJIFkfGm4aYT5	NVzzWlFKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NFc1PzRpPkK0O\|A4PDd2PD;hQi=>
HMEC-1	NFvlT5hHfW6ldHnvckBCe3OjeR?=	MoTON\|Axdk1?	MoDQNlRp		M2Pnfolv[3KnYYPld{BmgHC{ZYPzbY9vKG:owrDWSWdHWjQEoH3SUmE>	NWjrN\|VMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVA3OzFpPkK0O\|ExPjNzPD;hQi=>
HeLa	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NFrVRVAz\|ryP	NGjRN3U1QGh?		NGnpRVdl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgZYJwfXRiMkWl	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2NkGzOUc,OjR6NE[xN\|U9N2F-
LNCaP	NWTo[IdoT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MVSxNFAuOTByMH7N	MYmyOIg>		M3G2dGlEPTBib3[gN\|Axdk1?	M1rMOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEW0OlU5Lz5{NEi1OFY2QDxxYU6=
PC3	NXrrSVFtT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MWexNFAuOTByMH7N	MUmyOIg>		M3Xad2lEPTBib3[gN\|Axdk1?	M2HaOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEW0OlU5Lz5{NEi1OFY2QDxxYU6=
CNE2	NVn5bVVnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NUP2NFNbOTByLU[wNI5O	MX:yOE81QC95Mni=		MnrvbY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gbY4h[SC2aX3lMUBidmRiZH;z[U1l\XCnbnTlcpQhdWGwbnXy	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4OUmwNUc,OjR7Nkm5NFE9N2F-
HeLa	NUPNU4RqTnWwY4Tpc44hSXO\|YYm=	MmnhNlUxdk1?	NHTo[GUyPmh?	NYj4PZRrTE2VTx?=	MnS5bY5kemWjc3XzJGN[WDGDMTDtVm5CKGW6cILld5Nqd28EoB?=	NUXsPXBTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVY3QDhpPkK1NVE3Pjh6PD;hQi=>
A549	MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MYqwMlAyNTBwOd88US=>	NUHuU4JTPzKq	NYPnPZViTE2VTx?=	MYnJR\|UxKG:oIECuNFbPxE1?	MnTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkm0PVEoRjJ3MU[5OFkyRC:jPh?=
SKOV-3	NHXSWpFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MmrWNE4xOS1yLkpOwG0>	NX;2XYgzPzKq	MlS1SG1UVw>?	MWrJR\|UxKG:oIECuN\|LPxE1?	M2\I[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
A549	MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mny4NE4yNTIQvF2=	MmTiOFhp	M4DpN2ROW09?	NIizcXpKSzVyIH;mJFAvOjkQvF2=	NIDNfIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2PVQ6OSd-MkWxOlk1QTF:L3G+
SKOV-3	MmDWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NH\xU4kxNjFvMd88US=>	MUC0PIg>	NFriXFNFVVOR	Mn7NTWM2OCCxZjCwMlfPxE1?	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
A549	NGq5fpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NVzxR4ZROS1zMN88US=>	NUnhWlVrOjSq	MlLvSG1UVw>?	MV\JR\|UxKG:oIEOuNu69VQ>?	MknFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkm0PVEoRjJ3MU[5OFkyRC:jPh?=
SKOV-3	NYLzPZlmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NV3Jd2tEOS1zMN88US=>	M3rjclI1cA>?	M{\jXmROW09?	MX7JR\|UxKG:oIEWuOu69VQ>?	Mk\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzNkm0PVEoRjJ3MU[5OFkyRC:jPh?=
MCF7	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NHnWS4wxNTZyMH7N	MofxNlRp	NVXCbmVXTE2VTx?=	NHr6c5dKSzVyIH;mJFc2dk1?	NUe3OoxQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxPVI4OjFpPkK1NVkzPzJzPD;hQi=>
MDA-MB-231	NGDVd45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NFvYdmQxNTZyMH7N	NGXqc2UzPGh?	NY\BS4lnTE2VTx?=	MYjJR\|UxKG:oIEGwNI5O	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF7MkeyNUc,OjVzOUK3NlE9N2F-
A431	MXjGeY5kfGmxbjDBd5NigQ>?	M2j5TVUxdk1?	MVOyM\|YwOTJxMkTo	NUjyNIYxTE2VTx?=	M3jjdYFkfGm4YYTld{BxOjFiYX7kJIlvcGmkaYTzJGFVTjNiZYjwdoV{e2mxbh?=	NVj6XoY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzO\|ExPjlpPkK1N\|cyODZ7PD;hQi=>
A431	NXvJdYlQSXCxcITvd4l{KEG\|c3H5	MmTyNk8yOC93MD:xNFBvVQ>?	NIHkdJY1QGh?	M3\iTWROW09?	M13ZfolvcGmkaYTzJJRp\SClZXzsJIdzd3e2aB?=	NEj0e2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUO3NVA3QSd-MkWzO\|ExPjl:L3G+
PC3	M1LTemZ2dmO2aX;uJGF{e2G7	MYGwMlHPxE1?	M{LrT\|Q5cA>?	M3HaXGROW09?	M{HjZ4lv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ?	M4\vflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
SW480	Mne5SpVv[3Srb36gRZN{[Xl?	NIS3OpYxNjIQvF2=	NFmxeJI1QGh?	MoDCSG1UVw>?	NV\NNpR2cW6mdXPld{BqdmO{ZXHz[UBw\iCKRFHDNUBidmRiSFTBR\|Ihd25iU3z1[{Bo\W6nczDwdo9ud3Snch?=	M1XlTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
PC3	NWri[4pFTnWwY4Tpc44hSXO\|YYm=	MUiwMlHPxE1?	MmLtOFhp	MUHEUXNQ	NHfHW5hifHSnboXheIV{KGmwdnHzbY9vKGGwZDDtbYdz[XSrb36=	M2rwVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
PC3	NEOxempHfW6ldHnvckBCe3OjeR?=	NIjSbokxNjIQvF2=	NVrZSVgyPDiq	MVLEUXNQ	MlHhdoV3\XK\|ZYOgSW1V	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR\|NEm5O{c,OjV2M{S5PVc9N2F-
SW480	NGrnVnZHfW6ldHnvckBCe3OjeR?=	MYCwMlHPxE1?	M4DYd\|Q5cA>?	NFnqNVNFVVOR	MVTheJRmdnWjdHXzJIlvfmG\|aX;uJIFv\CCvaXfyZZRqd25?	M2D3RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
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melanoma B16/BL6	MlH6SpVv[3Srb36gZZN{[Xl?				NXzCSlltUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KGirc4TvcoUh\GWjY3X0fYxie2W\|IDjISGFEOSlicILldIFz\WRiZoLvcUBud3W\|ZTDt[Yxidm:vYTDCNVYwSkx4IHPlcIx{NCCLQ{WwJF0hOC5yMUmg{txONg>?	M2\jVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MUC5OlI3Lz5zNUGwPVYzPjxxYU6=
melanoma B16/BL6	MkLRSpVv[3Srb36gZZN{[Xl?				M4PBU2lvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKCiKRFHDOEkheHKncHHy[YQh\nKxbTDtc5V{\SCvZXzhco9u[SCEMU[vRmw3KGOnbHzzMEBKSzVyIE2gNE4xOiEQvF2u	NY\MR5JyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWxNFk3OjZpPkG1NVA6PjJ4PD;hQi=>
melanoma B16/BL6	NVnESXVLTnWwY4Tpc44h[XO\|YYm=				Ml:xTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIhqe3SxbnWg[IVi[2W2eXzhd4UhPiCycnXwZZJm\CCocn;tJI1wfXOnIH3lcIFvd22jIFKxOk9DVDZiY3XscJMtKEmFNUCgQUAxNjB{ODFOwG0v	MkDqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTVzMEm2NlYoRjF3MUC5OlI3RC:jPh?=
melanoma B16/BL6	NXrkeYRnTnWwY4Tpc44h[XO\|YYm=				M{e2SmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKDhicILldIFz\WRiZoLvcUBud3W\|ZTDt[Yxidm:vYTDCNVYwSkx4IHPlcIx{NCCLQ{WwJF0hOC5yNDFOwG0v	NF3id4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUGwPVYzPid-MUWxNFk3OjZ:L3G+
293T	NWm0W5hlTnWwY4Tpc44h[XO\|YYm=				M4e3U2lvcGmkaYTpc44hd2ZiaIXtZY4hcGm\|dH;u[UBl\WGlZYT5cIF{\SB2IIDy[ZBiemWmIH\yc40hOjl\|VDDj[YxteyxiSVO1NEA:KDBwMEKg{txONg>?	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR3NEKyOEc,OTV2NUSyNlQ9N2F-
293T	MYTGeY5kfGmxbjDhd5NigQ>?				NYjIeG5CUW6qaXLpeIlwdiCxZjDoeY1idiCqaYP0c45mKGSnYXPleJlt[XOnIEGgdJJmeGG{ZXSg[pJwdSB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6wNkDPxE1w	NH;kdZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUS1OFIzPCd-MUW0OVQzOjR:L3G+
293T	NWHJNXZQTnWwY4Tpc44h[XO\|YYm=				NFrMO4lKdmirYnn0bY9vKG:oIHj1cYFvKGirc4TvcoUh\GWjY3X0fYxie2ViNjDwdoVx[XKnZDDmdo9uKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkCyJO69VS5?	NXOwT4g5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0OVQzOjRpPkG1OFU1OjJ2PD;hQi=>
293T	MXHGeY5kfGmxbjDhd5NigQ>?				NHS0O3ZKdmirYnn0bY9vKG:oIHj1cYFvKGirc4TvcoUh\GWjY3X0fYxie2ViODDwdoVx[XKnZDDmdo9uKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkC0JO69VS5?	Ml3YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyNlQoRjF3NEW0NlI1RC:jPh?=
HeLa	MonjSpVv[3Srb36gZZN{[Xl?				NHjhdVNKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6\|dDDoeY1idiCqaYP0c45mKGSnYXPleJlt[XOnIDjISGFEMSCocn;tJGhmVGFiY3XscJMhf2m2aDDzeYJ{fHKjdHWgOYEtKEmFNUCgQUAxNjB3NEWg{txONg>?	NEHuTYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUS1OlI3Pyd-MUW0OVYzPjd:L3G+
HCT116	M2PvR2FvfGmycn;sbYZmemG2aY\lJIF{e2G7				MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFjDWFEyPiClZXzsd{whUUN3MDC9JFAvODBzMzFOwG0v	NF7JWpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmwOFg6OCd-MU[5NFQ5QTB:L3G+
H1299	NVnYPXgySW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				M17LUWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgTFEzQTliY3XscJMtKEmFNUCgQUAxNjFizszNMi=>	NFH2RnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmwOFg6OCd-MU[5NFQ5QTB:L3G+
H661	M17lRWFvfGmycn;sbYZmemG2aY\lJIF{e2G7				NXrGTZd3SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBJPjZzIHPlcIx{NCCLQ{WwJF0hOC5\|IN88UU4>	NVz4O29PRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NFQ5QTBpPkG2PVA1QDlyPD;hQi=>
H661	M3PpXGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NX;VS5pKPDhiaILz		M2\yWGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgTFY3OSClZXzsd{Bi\nSncjC0PEBpenNiYomgXHRVKGG\|c3H5MEBKSzVyIE2gNE4xQDVizszNMi=>	NXjpU3FORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2NlQ4PzNpPkG3OlI1Pzd\|PD;hQi=>
WiDr	NH;FOHNIem:5dHigbY5pcWKrdHnvckBie3OjeR?=				NEi2WWtKdmirYnn0bY9vKG:oIGPBVFE{OCCvZXTpZZRm\CClZXzsJIdzd3e2aDDpckBpfW2jbjDXbWRzKGOnbHzzMEBKSzVyIE2gNE42Pzd4IN88UU4>	NV;1WGRnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OFMyOTJpPkG3OlQ{OTF{PD;hQi=>
U251	NYD3bIE1TnWwY4Tpc44h[XO\|YYm=				NVzydXlMUW6qaXLpeIlwdiCxZjDTRXAyOzBiaX6gWmVITi2\|dHnteYxifGWmIHj1cYFvKFV{NUGgZ4VtdHNiYomgVGxCWCC{ZYDvdpRmeiCpZX7lJIF{e2G7LDDJR\|UxKD1iMD62NVEzKM7:TT6=	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ2M{GxNkc,OTd4NEOxNVI9N2F-
293T	MkXISpVv[3Srb36gZZN{[Xl?				M3XodmlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Gg[ZhxemW\|c3XkJIlvKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkCwNVkh\|ryPLh?=	M3j5bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEixNlMzLz5zN{i4NVI{OjxxYU6=
293T	NV7E[WdZTnWwY4Tpc44h[XO\|YYm=				NEfvVlhKdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO0JIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6wNFIh\|ryPLh?=	NFH0bFM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i4NVI{Oid-MUe4PFEzOzJ:L3G+
293T	NWHNTWsyTnWwY4Tpc44h[XO\|YYm=				MYjJcohq[mm2aX;uJI9nKG2xdYPlJGhFSUN4IHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yMEK4JO69VS5?	M4mwd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEixNlMzLz5zN{i4NVI{OjxxYU6=
H661	MoLLRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		NFXmeVA1QCCqcoO=		M3;nO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgTFY3OSClZXzsd{Bi\nSncjC0PEBpenNuIFnDOVAhRSByLkC4OUDPxE1w	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh7N{iyOEc,OTd6OUe4NlQ9N2F-
293T	MVfGeY5kfGmxbjDhd5NigQ>?				Ml\pTY5pcWKrdHnvckBw\iCKRFHDNUBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNVkh\|ryPLh?=	NUDWUlIzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
293T	NVjLZYJkTnWwY4Tpc44h[XO\|YYm=				Mk\QTY5pcWKrdHnvckBw\iCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNkDPxE1w	NYrCVHNHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
293T	MkHUSpVv[3Srb36gZZN{[Xl?				M2DVNWlvcGmkaYTpc44hd2ZiSFTBR\|Yh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOsJGlEPTBiPTCwMlI5KM7:TT6=	NX75b21sRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
NIH3T3	NH7LdFhHfW6ldHnvckBie3OjeR?=				Ml;PTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDNUBmgHC{ZYPz[YQhcW5iTlnIN3Q{KGOnbHzzMEBKSzVyIE2gNE4xOjFizszNMi=>	NFXhWYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{myPVc6QCd-MUe5Nlk4QTh:L3G+
human 293	MoDWSpVv[3Srb36gZZN{[Xl?				MnfoTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzJINmdGy\|LDDJR\|UxKD1iMD6wN\|Qh\|ryPLh?=	Ml7FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7Mkm3PVgoRjF5OUK5O\|k5RC:jPh?=
mouse 293	Mm\qSpVv[3Srb36gZZN{[Xl?				NWGzZXdiUW6qaXLpeIlwdiCxZjDtc5V{\SC{ZXPvcYJqdmGwdDDISGFEPiCneIDy[ZN{\WRiaX6gNlk{KGOnbHzzMEBKSzVyIE2gNE4xQDFizszNMi=>	NXyxVIVORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5Nlk4QThpPkG3PVI6Pzl6PD;hQi=>
SKBR3	NXG0WHBmSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				MXnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFONQmKzJINmdGy\|LDDJR\|UxKD1iMD6wNkDPxE1w	M1H2NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
MM96L	NGPpOFdHfW6ldHnvckBie3OjeR?=				M1rkZ2lvcGmkaYTpc44hd2ZiaIXtZY4hVU17NlygZ4VtdHNuIFnDOVAhRSByLkCzJO69VS5?	MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
SNU16	MX\GeY5kfGmxbjDhd5NigQ>?				MUHJcohq[mm2aX;uJI9nKEiGQVOg[pJwdSCqdX3hckBUVlVzNjDj[YxteyxiSVO1NEA:KDBwMEWzJO69VS5?	NVzFSJF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
A549	NIexcpFCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				NWKxfJBDSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzMEBKSzVyIE2gNE4xQCEQvF2u	M{XRPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
MKN45	NF3nRXJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=				M{DZOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVvOOFUh[2WubIOsJGlEPTBiPTCwMlEh\|ryPLh?=	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
SQ20B	MlTJSpVv[3Srb36gZZN{[Xl?				NYS3SYd7UW6qaXLpeIlwdiCxZjDoeY1idiCVUUKwRkBk\WyuczygTWM2OCB;IECuNkDPxE1w	MkL5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEe1OVQoRjF6MkS3OVU1RC:jPh?=
neonatal foreskin fibroblasts	MnfIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?				MorxS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gcoVwdmG2YXyg[o9z\XOtaX6g[oljem:kbHHzeJMh[2WubIOsJGlEPTBiPTCwMlIh\|ryPLh?=	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
HeLa	NEi5fYpHfW6ldHnvckBie3OjeR?=				NWfC[ZFVUW6qaXLpeIlwdiCxZjDISGFEKG[{b32gbJVu[W5iSHXMZUBk\WyuczygTWM2OCB;IECuN{DPxE1w	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MM96L/NFF	M2[2S2Z2dmO2aX;uJIF{e2G7				NH\T[IVU\WynY4Tpeol1gSCrbnTlfEwhemG2aX:gc4YhUUN3MDDmc5IhcHWvYX6gUW06PkxiY3XscJMhfG9iSVO1NEBnd3JiaIXtZY4hVk[IIHPlcIx{NCCLQ{WwJF0hPi55IN88UU4>	M1y3OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
293T	MYXGeY5kfGmxbjDhd5NigQ>?				M13CUGlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Gg[ZhxemW\|c3XkJIlvKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkCzOlYh\|ryPLh?=	NVrRZZVLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPVc5OjZpPkG4N\|k4QDJ4PD;hQi=>
293T	MWjGeY5kfGmxbjDhd5NigQ>?				M2j2fGlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Sg[ZhxemW\|c3XkJIlvKDJ7M2SgZ4VtdHNuIFnDOVAhRSByLkC2Nlkh\|ryPLh?=	NV\QXWRxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPVc5OjZpPkG4N\|k4QDJ4PD;hQi=>
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HeLa	MlXmR5l1d3SxeHnjbZR6KGG\|c3H5		MWS3NkBpenN?		Mm\5R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkGxJO69VS5?	Mnr4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkC2NlcoRjJ2OU[wOlI4RC:jPh?=
NCI-H1975	MV3DfZRwfG:6aXPpeJkh[XO\|YYm=		NIjPZmc4OiCqcoO=		NYPUTGVvS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVkOLLVixPVc2KGOnbHzzJIF{e2W\|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhS0ONODDhd5NigSxiSVO1NEA:KDBwMkGg{txONg>?	NVS4bo5XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA3OjdpPkK0PVYxPjJ5PD;hQi=>
K562	MkLOR5l1d3SxeHnjbZR6KGG\|c3H5		MkT6O\|IhcHK\|		MoPiR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT\|U3OiClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPzJiaILzJIJ6KEOFS{igZZN{[XluIFnDOVAhRSByLkSxJO69VS5?	NIrvc3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFYzPyd-MkS5OlA3Ojd:L3G+
HEK293	NUXxTFZuS3m2b4TvfIlkcXS7IHHzd4F6		NIqwO3Y6PiCqcoO=		M4fPWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhGUzJ7MzDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5zNjFOwG0v	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3NkGwNkc,OjV3NU[xNFI9N2F-
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HepG2	NXS1S2FZS3m2b4TvfIlkcXS7IHHzd4F6		MWK5OkBpenN?		NXfkOHN1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhQTZiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwOU[g{txONg>?	NGTnUYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1OlExOid-MkW1OVYyODJ:L3G+
U2OS	NHG1S5FEgXSxdH;4bYNqfHliYYPzZZk>		MoHtPVYhcHK\|		NYqwc5lTS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTKRUzDj[YxteyCjZoTldkA6PiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOTRwOUOg{txONg>?	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3NkGwNkc,OjV3NU[xNFI9N2F-
Sf9	MXLGeY5kfGmxbjDhd5NigQ>?		MVS5NEBucW6\|		M3H4V2lvcGmkaYTpc44hd2ZiZoXscEBt\W6pdHigbJVu[W5icnXjc41jcW6jboSgUk11\XKvaX7hcEBIW1RvdHHn[4VlKEiGQVO2JEgyKHSxIEGyNVUhemW\|aXT1[ZMqKGW6cILld5Nm\CCrbjDz[lkh[2WubIOgeZNqdmdiUljLMWspSWNrLVHNR{BieyC\|dXLzeJJifGViaX7jeYJifGWmIH\vdkA6OCCvaX7zJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMECxO\|E3KM7:TT6=	Mk[zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyNkC3OlQoRjJ5ME[wO\|Y1RC:jPh?=
Sf21	MUjGeY5kfGmxbjDhd5NigQ>?		NIjuVJA3OCCvaX7z		NGL1OXFKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJIh2dWGwIILlZ49u[mmwYX70JGMufGW{bXnuZYwhTkyDRz3IbZMufGGpZ3XkJGhFSUNzIDixJJRwKDR6MjDy[ZNq\HWnczmg[ZhxemW\|c3XkJIlvKFOoMkGgZ4VtdHNidYPpcochWkiNLVuoRYMqNUGPQzDhd{B{fWK\|dILheIUhcW6ldXLheIVlKG[xcjC2NEBucW6\|IHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvODB4NUe0JO69VS5?	M162UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5ME[wO\|Y1Lz5{N{C2NFc3PDxxYU6=
insect cell	MkTLSpVv[3Srb36gZZN{[Xl?		MXu2NEBucW6\|		MV\Jcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIHj1cYFvKHKnY3;tZolv[W62IFOteIVzdWmwYXygS3NVNXSjZ3fl[EBJTEGFMjCoNUB1dyB2OEigdoV{cWS3ZYOpJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgeZNqdmdiUljLMWspSWNrLVHNR{BieyC\|dXLzeJJifGViaX7jeYJifGWmIH\vdkA3OCCvaX7zJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMEGwOVEh\|ryPLh?=	NHLpRVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
insect cell	M1\te2Z2dmO2aX;uJIF{e2G7		M3rO[FYxKG2rboO=		MXfJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIHj1cYFvKHKnY3;tZolv[W62IFOteIVzdWmwYXygTIl{NXSjZ3fl[EBJTEGFMzCoNUB1dyB2MkigdoV{cWS3ZYOpM4h2dWGwIILlZ49u[mmwYX70JG4ufGW{bXnuZYwhT1OWLYTh[4dm\CCQQ1;SNkBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIGLIT{1MMEGlKT3BUWMh[XNic4Xid5Rz[XSnIHnuZ5Vj[XSnZDDmc5IhPjBibXnud{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkCxNVA4KM7:TT6=	M2L1clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5ME[wO\|Y1Lz5{N{C2NFc3PDxxYU6=
insect cell	MkjvSpVv[3Srb36gZZN{[Xl?				NHraVYpKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IF6teIVzdWmwYXygTIl{NXSjZ3fl[EBJTEGFMUGgLFEhfG9iM{S3JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzM4Ji[3Wub4\pdpV{KGW6cILld5Nqd25ic4nzeIVuKHW\|aX7nJHJJUy2NKFHjLU1CVUNiYYOgd5Vje3S{YYTlJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMEGxN\|ch\|ryPLh?=	MlP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyNkC3OlQoRjJ5ME[wO\|Y1RC:jPh?=
insect cell	M{fleWZ2dmO2aX;uJIF{e2G7		MkjzOlAhdWmwcx?=		NEfSXpVKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFOteIVzdWmwYXygTIl{NXSjZ3fl[EBJTEGFODCoNUB1dyB\|N{egdoV{cWS3ZYOpJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOgeZNqdmdiUljLMWspSWNrLVuoRYMqNUGPQzDhd{B{fWK\|dILheIUhcW6ldXLheIVlKG[xcjC2NEBucW6\|IHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvOzB5NDFOwG0v	MknqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyNkC3OlQoRjJ5ME[wO\|Y1RC:jPh?=
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insect cell	NFHrXZBHfW6ldHnvckBie3OjeR?=		Ml7TOEBpenN?		NYTN[IdoUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEPiCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhcW6\|ZXP0JINmdGy\|IIXzbY5oKEKDVFPQJIF{KHO3YoP0doF1\SCjZoTldkA1KGi{czDifUBWUFCOQz3FV2kuVVNxTWOgZY5idHm\|aYOsJGlEPTBiPTCwMlAyPiEQvF2u	Mmj3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4NUC5NlUoRjJ5NkWwPVI2RC:jPh?=
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HEK293T	NIDlTYhHfW6ldHnvckBie3OjeR?=		MXOzNEBucW6\|		MULJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IH3veZNmKEiGQVO2JIV5eHKnc4Pl[EBqdiCKRVuyPVNVKGOnbHzzJINwNWW6cILld5NqdmdibX;1d4UhUESDMjD1d4lv\yCDYz3LS2xIUyiDYzmtUWNCKGG\|IIP1ZpN1emG2ZTDh[pRmeiB\|MDDtbY5{KGmwIIDy[ZNmdmOnIH;mJGRVXCCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYmsJGlEPTBiPTCwMlAzQCEQvF2u	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTVzOU[wOEc,Ojl3MUm2NFQ9N2F-
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insect cell	MkH5SpVv[3Srb36gZZN{[Xl?				MkHoTY5pcWKrdHnvckBw\iCQLYTldo1qdmGuIFfTWE11[WepZXSgdoVkd22kaX7hcpQhcHWvYX6gTGRCSzZiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwZnXjeIVlKGmwc3XjeEBk\WyuczD1d4lv\yCUSFvLLGFkMUGPQzDhd{B{fWK\|dILheIUh[nliZnz1c5Jme2OnbnPlMYJie2WmIHHzd4F6NCCLQ{WwJF0hOC5yMEi1JO69VS5?	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN4NUi5Nkc,OzB\|NkW4PVI9N2F-
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insect cell	MYPGeY5kfGmxbjDhd5NigQ>?				MWHJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEiGQVOzM25EV1J{IHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2eyCrbn\lZ5Rm\CCrboPlZ5Qh[2WubIOgeZNqdmdiUljLT{hC[ymDTVOgZZMhe3Wkc4TyZZRmKGK7IH\seY9z\XOlZX7j[U1j[XOnZDDhd5NigSxiSVO1NEA:KDBwMEGyO{DPxE1w	NHnBU4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9\|MEO2OVg6Oid-M{CzOlU5QTJ:L3G+
insect cell	NFPPPJdHfW6ldHnvckBie3OjeR?=				NVf6NZAxUW6qaXLpeIlwdiCxZjDDMZRmem2rbnHsJGdUXC22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hUESDQ{Kg[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC\|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR\|UxKD1iMD6wNlY4KM7:TT6=	NVnQRXlHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlU5QTJpPkOwN\|Y2QDl{PD;hQi=>
insect cell	NV;HNHBGTnWwY4Tpc44h[XO\|YYm=				NVj0[ok5UW6qaXLpeIlwdiCxZjDDMZRmem2rbnHsJGhqey22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hUESDQ{ig[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC\|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR\|UxKD1iMD62NFkh\|ryPLh?=	NYj2VoRzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlU5QTJpPkOwN\|Y2QDl{PD;hQi=>
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Hela	MoPWSpVv[3Srb36gZZN{[Xl?				MljITY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhmdGFiY3XscJMhdnWlbHXhdkBmgHS{YXP0d{BjgSCobIXvdolu\XS{aXOgZZN{[XluIFvpJF0hOC53MESg{txONg>?	NVnBbG57RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1NlA2QDBpPkG5OVIxPThyPD;hQi=>
Sf9	M1;GUGZ2dmO2aX;uJIF{e2G7		NGX6OlA6OCCvaX7z		MWLJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIILlZ49u[mmwYX70JIh2dWGwIF6teIVzdWmwYXygS3NVNXSjZ3fl[EBJTEGFNjDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiU3[5JIlve2WldDDj[YxteyC3c3nu[{BTUEuNKFHjLWFOSyCjczDzeYJ{fHKjdHWgZYZ1\XJiOUCgcYlveyCkeTDmcJVwemmvZYTldkwhU2liPTCwMlAxODV6IN88UU4>	MkPBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
Sf21	NXjaclVKTnWwY4Tpc44h[XO\|YYm=		MX[2NEBucW6\|		MlnSTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCFLYTldo1qdmGuIF\MRWcwUGm\|LYTh[4dm\CCKRFHDNUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOtbY5n\WO2ZXSgV4YzOSCrboPlZ5Qh[2WubIOgeZNqdmdiUljLT{hC[ymDTVOgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkh\my3b4LpcYV1\XJuIFvpJF0hOC5yMEC2OEDPxE1w	M2q3XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
insect cell	NVrNXmlrTnWwY4Tpc44h[XO\|YYm=		NHf6Z3Y3OCCvaX7z		MWHJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIILlZ49u[mmwYX70JIh2dWGwIFOteIVzdWmwYXygTIl{NXSjZ3fl[EBJTEGFMz;OMZRmem2rbnHsJGdUXC22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hVkORUkKgLFM6PSC2bzC0PFkhemW\|aXT1[ZMqKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{1qdm[nY4Tl[EBqdnOnY4SgZ4VtdHNidYPpcochWkiNSzjBZ{lCVUNiYYOgd5Vje3S{YYTlJIFnfGW{IE[wJI1qdnNiYomg[ox2d3KrbXX0[ZItKEurIE2gNE4xODB6NDFOwG0v	MlfrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
insect cell	M1PVb2Z2dmO2aX;uJIF{e2G7		MmPLOlAhdWmwcx?=		NXewNZhOUW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBz\WOxbXLpcoFvfCCqdX3hckBENXSncn3pcoFtKEeVVD30ZYdo\WRiSFTBR\|Ih\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzMYlv\mWldHXkJIlve2WldDDj[YxteyC3c3nu[{BTUEuNKFHjLWFOSyCjczDzeYJ{fHKjdHWgZYZ1\XJiNkCgcYlveyCkeTDmcJVwemmvZYTldkwhU2liPTCwMlAxOzV3IN88UU4>	NGX1cIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
insect cell	NImxR\|hHfW6ldHnvckBie3OjeR?=				MWHJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEiGQVOxNEBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOtbY5n\WO2ZXSgbY5{\WO2IHPlcIx{KHW\|aX7nJIZtfW:{b3flcolkKHCncITp[IUhWkiNS1HjJIF{KHO3YoP0doF1\SCkeTDmcJVwemmvZYTldkwhU2liPTCwMlAxPDd4IN88UU4>	NETKN3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
insect cell	MkHBSpVv[3Srb36gZZN{[Xl?		M2WyfFYxKG2rboO=		MmjzTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDoeY1idiCFLYTldo1qdmGuIFjpd{11[WepZXSgTGRCSzhiKEGgeI8hOzd5IILld4llfWW\|KTDlfJBz\XO\|ZXSgbY4h[mGldXzveolzfXNvaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT{hC[ymNKFHjLWFOSyCjczDzeYJ{fHKjdHWgZYZ1\XJiNkCgcYlveyCkeTDmcJVwemmvZYTldkwhU2liPTCwMlE1PSEQvF2u	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6OUS0NUc,Ojl3OEm0OFE9N2F-
insect cell	MoTJSpVv[3Srb36gZZN{[Xl?				NGf5[HJKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIF6teIVzdWmwYXygTIl{NXSjZ3fl[EBJTEGFMUGgLFEhfG9iM{S3JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOtbY5n\WO2ZXSgbY5{\WO2IHPlcIx{KHW\|aX7nJHJJU0tqQXOpRW1EKGG\|IIP1ZpN1emG2ZTDifUBndHWxcnnt[ZRmeixiS3mgQUAxNjRyMTFOwG0v	NFjPblQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
GM15850	MkfySpVv[3Srb36gZZN{[Xl?	MV[wMlEhfU1?	MnvqNVIhcHK\|		M3jxT2lvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBIVTF3OEWwJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKHSxdHHsJIFk\XS7bHH0[YQhcGm\|dH;u[UBt\X[nbDDheEAxNjFidV2gZYZ1\XJiMUKgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=>	NEHOe5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
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HEK293	MUHGeY5kfGmxbjDhd5NigQ>?	Mon6OlAxKG6P			MWrJcoNz\WG\|ZTDpckBxem:2ZXnuJIRqe3WuZnnk[UBqe2:vZYLhd4UhdGW4ZXygbY4hUEWNMkmzJINmdGy\|IHH0JFYxOCCwTTDifUBqdW23bn;icI91	NYqwN2JLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1OlY4OzJpPkG3OVY3PzN{PD;hQi=>
HeLa	NX3ZXHVWTnWwY4Tpc44h[XO\|YYm=	MYexJJVO	M{G3U\|I1KGi{cx?=		MX\JcoR2[3Srb36gc4YheDF4IHX4dJJme3Orb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCBzIIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5R1cW6p	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ2M{GxNUc,OTd4NEOxNVE9N2F-
HeLa	NX3RXIJ2TnWwY4Tpc44h[XO\|YYm=	M3e5SlEhfU1?	NGHqeWQzPCCqcoO=		NHzUSY1KdmS3Y4Tpc44hd2ZicEK3JIV5eHKnc4Ppc44hcW5iaIXtZY4hUGWOYTDj[YxteyCjdDCxJJVOKGGodHXyJFI1KGi{czDifUBY\XO2ZYLuJIJtd3S2aX7n	MoL3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NEOxNVEoRjF5NkSzNVEyRC:jPh?=
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HCT116	NFXNXVBHfW6ldHnvckBie3OjeR?=				M{XJfGlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{GgZZN{\XO\|ZXSgZZMhcGm\|dH;u[UBpgXCncnHj[ZR6dGG2aX;uJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCkeTDX[ZN1\XKwIHLsc5Q>	MoPSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNUSyN\|koRjF6MEW0NlM6RC:jPh?=
HCT116	M2XOU2Z2dmO2aX;uJIF{e2G7				M1;Fc2lvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{[gZZN{\XO\|ZXSgZZMhfHWkdXzpckBpgXCncnHj[ZR6dGG2aX;uJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCkeTDX[ZN1\XKwIHLsc5Q>	MmXsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNUSyN\|koRjF6MEW0NlM6RC:jPh?=
HEK293 STF	MnLKSpVv[3Srb36gZZN{[Xl?		NYrVWJBlOjRiaILz		MmPORYN1cX[jdHnvckBw\iCvb4Xz[UBYdnR\|YTDzbYdv[Wyrbneg[ZhxemW\|c3XkJIlvKEiHS{K5N{BUXEZiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6geJJidnOlcnnweIlwdmGuIHHjeIl3cXS7IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUu[mG\|ZXSgcIFkfGG2ZTDk[Yh6\HKxZ3XuZZNmKGG\|c3H5	NUHpZpE2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwNlI3PzVpPkG5NFIzPjd3PD;hQi=>
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HL60	NX7LcYFGTnWwY4Tpc44h[XO\|YYm=	MlvTNlAhfWdxbXy=	MnLRNlQhcHK\|		MkjtTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhNPjBiY3XscJMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIN{Bi[2W2eXzheIlwdiCjdDCyNEB2\y:vbDDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	NEDTOZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUWzOFQ4OSd-MUm1N\|Q1PzF:L3G+
primary bronchial epithelial cell	M1;KUmZ2dmO2aX;uJIF{e2G7	NY\xZ49WOC5zIIXN			M4TOUWlvcGmkaYTpc44hd2ZiSFTBR\|chcW5iaIXtZY4heHKrbXHyfUBjem:wY3jpZYwh\XCrdHjlcIlidCClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gcZV1[W62IF\k[Yx1[TVyODDDSnRTKHC{b4TlbY4h[XCrY3HsJJN2em[jY3WgcI9k[WyrenH0bY9vKGG2IECuNUB2VQ>?	M13E[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OU[2O\|g6Lz5zOUm2Olc5QTxxYU6=
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HeLa	MX\GeY5kfGmxbjDhd5NigQ>?	MWCxJJVO	NIHBXWoyOCCvaX7z		Mn[1TY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMTD1UUBi\nSncjCxNEBucW6\|IHL5JIZtfW:{b33leJJq[yCjc4PhfS=>	M{Lub\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEW1PFQ5Lz5{MUC1OVg1QDxxYU6=
MCF7	NILBZ4JHfW6ldHnvckBie3OjeR?=	MYmwMlUhfU1?	MmnzNlQhcHK\|		M17JdmlvcGmkaYTpc44hd2ZiQ3zhd5MhOSCqaYP0c45mKGSnYXPleJlt[XOnIHnuJIh2dWGwIF3DSlch[2WubIOgZZN{\XO\|ZXSgZZMhfXBvcnXneYxifGmxbjDv[kBxOjFicILveIVqdiCuZY\lcEBifCByLkWgeW0h[W[2ZYKgNlQhcHK\|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>?	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6MEO2N{c,OjJ{OECzOlM9N2F-
MCF7	MY\GeY5kfGmxbjDhd5NigQ>?	MWmwMlUhfU1?	M{fnPFI1KGi{cx?=		Mo\zTY5pcWKrdHnvckBw\iCKRFHDOkBqdiCqdX3hckBOS0Z5IHPlcIx{KGG\|c3Xzd4VlKGG\|IHPoZY5o\SCrbjD0eYJ2dGmwIHHj[ZR6dGG2aX;uJIxmfmWuIHH0JFAvPSC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	M3LWcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkiwN\|Y{Lz5{MkK4NFM3OzxxYU6=
MCF7	NHHscmFHfW6ldHnvckBie3OjeR?=	M2rseVAvPSC3TR?=	NVvYPIRROjRiaILz		M3KwU2lvcGmkaYTpc44hd2ZiSFTBR\|EhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczD1dE1z\We3bHH0bY9vKG:oIHHj[ZR6dGG2ZXSgbIl{fG:wZTDIN{Bxem:2ZXnuJIxmfmWuIHH0JFAvPSC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	MnXiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{OECzOlMoRjJ{MkiwN\|Y{RC:jPh?=
PC12	NX:0VWpJTnWwY4Tpc44h[XO\|YYm=	M2jQVlExKHWP	NHPpWlQ1QCCqcoO=		MoS4TY5pcWKrdHnvckBw\iCKRFHDOkBqdiC{YYSgVGMyOiClZXzsd{Bie3Onc4Pl[EBieyCjY3X0fYxifGmxbjDv[kBpcXO2b37lJGg{KGG2IEGwJJVOKGGodHXyJFQ5KGi{czDifUBY\XO2ZYLuJIJtd3S2aX7nJIFv[Wy7c3nz	NYWxRVdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
PC12	NHu4SWZHfW6ldHnvckBie3OjeR?=	NVPZZ4p[OTBidV2=	M33tPVQ5KGi{cx?=		M4LRd2lvcGmkaYTpc44hd2ZiSFTBR\|YhcW5icnH0JHBEOTJiY3XscJMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFteGijLYT1ZpVtcW5iYXPleJlt[XSrb36gZZQhOTBidV2gZYZ1\XJiNEigbJJ{KGK7IGfld5Rmem5iYnzveJRqdmdiYX7hcJl{cXN?	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzlyNU[4NEc,OjN7MEW2PFA9N2F-
PC12	MXrGeY5kfGmxbjDhd5NigQ>?	NEf0eZMyOCC3TR?=	MVO1JIRigXN?		M4fvWmlv\HWldHnvckBw\iCwZYXybZRmKG:3dHfyc5d1cCCrbjDyZZQhWENzMjDj[YxteyCjdDCxNEB2VSCjZoTldkA2KGSjeYO=	NYW1ZWxyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
SH-SY5Y	NYXs[3pVTnWwY4Tpc44h[XO\|YYm=	MWWxNEB2VQ>?	NHnifm82KGSjeYO=		NH;kcHBKdmS3Y4Tpc44hd2ZibnX1dol1\SCxdYTndo94fGhiaX6gbJVu[W5iU1itV3k2YSClZXzsd{BifCBzMDD1UUBi\nSncjC1JIRigXN?	NXrsOJpPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
A549	MorPRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?	MUGwMlQhfU1?	MXSyOEBpenN?		M{m4c2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{B{cHK3bnvlckBidmRic3jhdpAhe2ijcHWgZZQhOC52IIXNJIFnfGW{IEK0JIhzeyCkeTDwbIF{\SClb370doF{fCCvaXPyc5Nkd3C7	NEXKb4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUi4OFEyOid-MkW4PFQyOTJ:L3G+
HeLa	MYjGeY5kfGmxbjDhd5NigQ>?				NFvMRmVKdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgeJVjfWyrbjDhZ4V1gWyjdHnvckBqdmO3YnH0[YQh\m:{IH;2[ZJvcWeqdDDifUBqdW23bn;mcJVwemW\|Y3XuZ4UhdWmlcn;zZ49xcWNiYX7hcJl{cXN?	MkTyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ4MUG5NVkoRjJ4NkGxPVE6RC:jPh?=
C127-LT	M2XqS2Z2dmO2aX;uJIF{e2G7	M1vmbFIxOCCwTR?=	NX[1THFEOjRiaILz		NF;zZndKdmirYnn0bY9vKG:oIFjERWMhcW5ibX;1d4UhSzF{Nz3MWEBk\WyuczDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIFXHSnAh\XiycnXzd4lwdiCjdDCyNFAhdk1iYX\0[ZIhOjRiaILzJIJ6KG[udX;y[ZNk\W6lZTDtbYNzd3Olb4D5	NGHhenc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OlM4Oid-Mk[5PVY{PzJ:L3G+
HeLa nuclear	MmjISpVv[3Srb36gZZN{[Xl?	NHvBNGQyOCC2bzCyOUB2VQ>?	NH64fJI{OCCvaX7z		NI\TXmpEd22yZYTpeIl3\SCrbnjpZol1cW:wIH;mJGhFSUNiaX6gbJVu[W5iSHXMZUBvfWOuZXHyJINmdGy\|IHH0JFExKHSxIEK1JJVOKHW\|aX7nJGJw[y2jY3X0fYwudHm\|aX7lMWFOSyCjczDzeYJ{fHKjdHWgZYZ1\XJiM{CgcYlveyCkeTDMbY5mf2WjdnXyMWJ2emticHzveEBidmGueYPpdy=>	M1nyWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUm2N\|czLz5{Nkm5OlM4OjxxYU6=
HeLa	M3rm[2Z2dmO2aX;uJIF{e2G7	M4nGblIhfU1?	NYjZelYxOjRiaILz		MVnJcohq[mm2aX;uJI9nKEiGQVOxJIlvKGi3bXHuJGhmVGFiY3XscJMh[XO\|ZYPz[YQh[XNiaInw[ZJi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KDJidV2gbY5kfWKjdHXkJIZweiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	NGrxUW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
HN13	NIXsV4JHfW6ldHnvckBie3OjeR?=		MUKyOEBpenN?		MWHJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTG4yOyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gbIl{fG:wZTDIN{BNgXN7IHHj[ZR6dGG2aX;uJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHntcZVvd2[udX;y[ZNk\W6lZTDhd5NigQ>?	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB\|MUC2Okc,OjlyM{GwOlY9N2F-
HeLaS3	NXe4e5dSTnWwY4Tpc44h[XO\|YYm=	NGTte2UyOCC3TR?=			M4Phc2lvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJ\UyjU{OgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcGm\|dH;u[UBJO0t7IHHj[ZR6dGG2aX;uJIF1KDFyIIXNJI1m[XO3cnXkJIludWWmaXH0[Yx6KGK7IHntcZVvd2Kub4SgZY5idHm\|aYO=	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N\|U9N2F-
HeLaS3	NGPGenZHfW6ldHnvckBie3OjeR?=	MYOxNEB2VQ>?	MYOxOkBpenN?		MXXJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVN[VN\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFNMQSCjY3X0fYxifGmxbjDheEAyOCC3TTD0doVifGWmIH\vdkAyPiCqcoOg[o9tdG:5ZXSgZpkh[2:vcH;1coQhf2G\|aH;1eEBidmRibXXhd5Vz\WRidYCgeI8hOjRiaILzJIJ6KGmvbYXuc4Jtd3RiYX7hcJl{cXN?	MmLiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NUi1N\|UoRjNyMkW4OVM2RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	EGFR / STAT3 ; PubMed: 31289542 Oncol Lett Expression of EGFR and STAT3 in PC3 cells treated with TSA. PC3 cells were plated in 6-cm dishes, and 0.25, 0.5 or 1 µM TSA was added for 24 h. DMSO was added into the control group wells. Cells were collected and lysed with lysis buffer and the protein expression analyzed via western blotting. Cyclin D1 / CDK6 / CDK4 / p-RB / RB; PubMed: 31289542 Oncol Lett Expression of cyclin D1, CDK6, CDK4 and RB, and the phosphorylation of RB, was detected in PC3 cells treated with TSA for 24 h. MMP-1 / MMP-3 / MMP-13 / TIMP-1; PubMed: 27431944 Mol Med Rep Chondrocytes were pretreated with increasing concentrations of TSA for 2 h, followed by stimulation with 5 ng/ml IL-1β for 24 h. Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1); PubMed: 27431944 Mol Med Rep Chondrocytes were treated with increasing concentrations of TSA for 24 h. α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2; PubMed: 23802098 Front Oncol Histone acetylation occurs directly at the promoter region of IL-23R. The ChIP assay demonstrates that TSA treatment results in an increase in the acetylation of histone H3 and H4. A549 cells were cultured in the presence or absence of TSA (250 ng/mL) for a period of 24 h. Subsequently, a ChIP assay was performed using the following antibodies; pan acetyl-histone H3 (H3Ac), pan acetyl-histone H4 (H4Ac), acetyl-histone H3 Lys 9 (H3K9Ac), acetyl-histone H3 Lys 9/14 (H3K9/14ac), acetyl-histone H3 Lys 9 phosphoSer10 (H3K9S10), methyl-histone H3 Lys 4 (H3K4Me), di methyl-histone H3 Lys 4 (H3K4Me2), and di methyl-histone H3 Lys 9 (H3K9Me2). Input DNA serves as a positive control as recommended by the manufacturer (Diagenode). A no antibody control was included to test for non-specific binding (UT, untreated; TSA, Trichostatin A).	31289542 27431944 23802098
Immunofluorescence	α-C/EBPβ / α-HP1α ; PubMed: 21122806 Exp Cell Res 3T3-L1 preadipocytes were grown on coverslips for three days and induced to differentiate by treatment with MDI (48 h) in the presence of vehicle (-TSA) or 87 nM Trichostatin A (+TSA). Under these experimental conditions: A-H Cells were fixed and permeabilized in cold methanol, subjected to IIF with the indicated antibodies, and nuclei were counterstained with DAPI. Scale bar, 5 μm. α-HDAC1 ; PubMed: 29045501 PLoS One MCF-7 cells were treated with indicated concentrations of trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels. α-tubulin / acetylated histone 3; PubMed: 27957719 Neurotherapeutics Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775.	21122806 29045501 27957719
Growth inhibition assay	Cell viability (A549 cells); PubMed: 27571418 PLoS One Cytotoxicity of histamine determined by MTT assay in A549 cells. Cell viability (MDA-MB-231 cells); PubMed: 27548148 Int J Mol Sci The human breast cancer cells (MDA-MB-231) were incubated with various concentrations of TSA (0–300 nM) for 24 h. Cell viability was measured by WST-8. Cell viability (PC3 cells); PubMed: 31289542 Oncol Lett PC3 cells were plated in a 96-well plate and treated with different doses of TSA (0, 0.25, 0.5 and 1 µM) for 72 h; an MTT assay was used to measure the effects of TSA on PC3 cell proliferation.	27571418 27548148 31289542

体内研究

Administration of Trichostatin A 按 0.5 mg/kg 剂量处理N-甲基-N-亚硝基脲致癌物诱发的大鼠乳腺癌模型，持续处理4周，具有有效的抗癌活性，即使按按高达5 mg/kg剂量处理也没有任何可测量到的毒性。^[1]Trichostatin A 按10 mg/kg 剂量单独腹腔注射给药非转基因和脊髓性肌萎缩症（SMA）模型鼠，导致乙酰化的 H3 和 H4组蛋白水平提高，也导致活运动神经元(SMN) 基因表达稍微提高。Trichostatin A 每天按10 mg/kg剂量处理SMA模型鼠，促进存活, 减轻体重下降,且增强运动行为。^[5]

激酶实验：^[1]	- 合并体外HDAC活性 : 从每种乳腺癌细胞系(MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51,或 SK-BR-3)中准备全部细胞提取物。在溶于0.1% (v/v)乙醇的不同浓度Trichostatin A存在时，或者使用0.1% (v/v)乙醇作为对照, 20 μL 细胞粗提取物(~2.5 ×10⁵ 个细胞)与 1 μL ~1.5 × 10⁶ cpm [³H]乙酰标记的组蛋白 H4 肽底物(第2-20位NH2-末端残基) 在25^oC下温育60分钟。使用50 μL 1 M HCl/0.16 M 乙酸对每组 200-μL 反应进行淬火，然后使用600 μL 乙酸乙酯进行抽提，通过闪烁计数器测量释放的[³H]乙酸。使用非线性回归曲线拟合抑制数据，绘制合适的剂量反应曲线，而测定IC50。
细胞实验：^[1]	- 合并 Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3 Concentrations: 溶于无水酒精,终浓度为~10 μM Incubation Time: 96小时 Method: 使用不同浓度Trichostatin A处理细胞96小时。处理后,使用sulforhodamine B 比色分析测定细胞增殖。通过台酚蓝染色排除法测定细胞活力。 (Only for Reference)
动物实验：^[1]	- 合并 Animal Models: 携带NMU诱导的肿瘤的近交系(Ludwig/Wistar/Olac)处女鼠 Dosages: ~5 mg/kg/day Administration: 皮下注射 (Only for Reference)

参考文献

- 合并

溶解度 (25°C)

体外	DMSO	23 mg/mL (76.05 mM)
	Water	Insoluble
	Ethanol	Insoluble
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+2% Tween 80+ddH2O	1.5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Trichostatin A (TSA) SDF

分子量	302.4
化学式	C₁₇H₂₂N₂O₃
CAS号	58880-19-6
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	N/A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、SDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2022-01-17)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04606771	Recruiting	Drug: Osimertinib + Savolitinib\|Drug: Savolitinib + Placebo	Non-Small Cell Lung Cancer	AstraZeneca	September 28 2020	Phase 2
NCT03901053	Recruiting	Device: Reaktiv AFO\|Device: PhatBrace AFO	Foot Injuries and Disorders	Jason Wilken\|Minneapolis Veterans Affairs Medical Center\|Walter Reed National Military Medical Center\|Henry M. Jackson Foundation for the Advancement of Military Medicine\|Center for Veterans Research and Education\|University of Delaware\|Johns Hopkins Bloomberg School of Public Health\|University of Iowa	February 27 2020	Not Applicable
NCT03887650	Recruiting	Drug: Liposomal Bupivicaine 1.3%\|Drug: Bupivacaine 0.5%	Post-operative Pain\|Total Shoulder Arthroplasty\|Osteoarthritis of the Shoulder\|Pain Management	Hartford Hospital	January 14 2019	Phase 4

技术支持

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操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?
回答：
Our S1045 Trichostatin A (TSA) is R enantiomer.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

选择性强的HDAC抑制剂

Rocilinostat (ACY-1215) : HDAC5-selective, IC50=5 nM.
活性最强的HDAC抑制剂

Quisinostat (JNJ-26481585) : HDAC1, IC50=0.11 nM; HDAC2, IC50=0.33 nM.
FDA批准的HDAC抑制剂

Vorinostat (SAHA, MK0683) : Approved by FDA against cutaneous T cell lymphoma.
最新的HDAC抑制剂

RG2833 (RGFP109) ^New : Brain-penetrant HDAC inhibitor with IC50 of 60 nM and 50 nM for HDAC1 and HDAC3, respectively.

研究领域

产品类型

定制您的化合物库

Trichostatin A (TSA)

客户使用Selleck生产的Trichostatin A (TSA)发表文献211篇：

产品安全说明书

HDAC抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Trichostatin A (TSA) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2022-01-17)

技术支持

常见问题及建议解决方法

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

相关HDAC产品