Trichostatin A (TSA)

For research use only. Not for use in humans.

目录号:S1045 中文名称:曲古柳菌素A

Trichostatin A (TSA) Chemical Structure

CAS No. 58880-19-6

Trichostatin A (TSA)是一种HDAC抑制剂,无细胞试验中IC50为1.8 nM左右。

规格 价格 库存 购买数量  
RMB 2022.93 现货
RMB 5053.26 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Trichostatin A (TSA)发表文献179篇:

产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述 Trichostatin A (TSA)是一种HDAC抑制剂,无细胞试验中IC50为1.8 nM左右。
靶点
HDAC [1]
(Cell-free assay)
~1.8 nM
体外研究

Trichostatin A 抑制八种乳腺癌细胞系,包括MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3增殖,平均IC50为124.4 nM (范围为26.4-308.1 nM),作用于表达ERα的细胞系比作用于表达 ERα阴性的细胞系更有效。Trichostatin A作用于全部乳腺癌细胞系,抑制HDAC活性,平均IC50为 2.4 nM (范围为0.6-2.6 nM), 产生显著的组蛋白 H4高度乙酰化。[1]与 Trapoxin (TPX) 和 Chlamydocin 有效抑制HDAC1 或 HDAC4而不是 HDAC6不同,Trichostatin A 抑制这些HDACs,且抑制程度相似, IC50 分别为6 nM, 38 nM, 和8.6 nM。[2] Trichostatin A (100 ng/mL) 作用于MIA PaCa-2细胞,通过招募p300和 PCAF进入Sp1-NF-Y HDAC复合体,而诱导转化生长因子βⅡ型受体(TβRII) 表达,复合体与TβRII 启动子的DNA片段结合,伴随着Sp1乙酰化,且与复合体相关的HDAC数理全部降低。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa NV\G[2VVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MorhNVAwOzBxNUCgcm0> M3;KflczKGh? M1ftbGROW09? MVTpcoNz\WG|ZYOgeIhmKG63bXLldkBw\iCPTWCgLO6V|qivKTDsc5N{KGOnbHzzJIRwe2ViZHXw[Y5l\W62bIm= Mnf5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNkW3OFgoRjJ|MU[1O|Q5RC:jPh?=
HeLa NX7sXmhVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrzNWFlOjBibl2= NXPOfFRJPzJiaB?= NX6zWZR2TE2VTx?= MU\JR|UxKG:oIEKwcm0> NUXidYhwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa NFnwVVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXHBfIgyPDBibl2= M2jQRVQ5KGh? MXzEUXNQ NET2VYhKSzVyIH;mJFQxdk1? NYDtNm9ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa NYfUUZFUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXGxNFAhdk1? NXfa[JJ5OjRiaB?= MmXwSG1UVw>? M3OxdmlEPTBib3[gNVAxdk1? MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF4NUe0PEc,OjNzNkW3OFg9N2F-
Caco-2  MVjGeY5kfGmxbjDBd5NigQ>? M4\JU|EhyrWPwrC= NFy5bHIzPCCq MlLK[IVkemWjc3XzJHNGWlRicILveIVqdiCneIDy[ZN{cW:w MlXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzOUWwO|AoRjJ|MUm1NFcxRC:jPh?=
HTK NGPuWWRHfW6ldHnvckBCe3OjeR?= M1LnO|ExOC16MEDuUS=> NGLORoE4OiCq M4LCeYJtd2OtczDUS2Yu|rMkgKPJcoR2[2WmIGLPV{BidmRiSELPNuKhSWOldX31cIF1cW:w NILMR3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4OFAxOid-MkOyPFQxODJ:L3G+
HTK MmP6SpVv[3Srb36gRZN{[Xl? MWK0NFAhdk1? NWLFS5Y5PzJiaB?= NEnlTlJqdmirYnn0d{BVT0ZvzsNihLNKdmS3Y3XkJG16d2[rYoLvZoxie3RiRHnm[oVz\W62aXH0bY9v MorJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{OESwNFIoRjJ|Mki0NFAzRC:jPh?=
HEK293 MYPGeY5kfGmxbjDBd5NigQ>? NFPr[4kyKML3TdMg M3T0[|E5KGkEoB?= M4DDdolvcGmkaYTzJJRp\SCtYXzpdolvNTdvbXXkbYF1\WRicnXjdpVqfG2nboSgc4Yhe3mwcHjpcIlvNTFiYXfndoVo[XSnczDpcpRwKGGpZ4Lld49u\XN? M2DGOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|Mki0PFQ5Lz5{M{K4OFg1QDxxYU6=
SH-SY5Y NUHpcJo1TnWwY4Tpc44hSXO|YYm= M3jn[|I2OCCwTR?= MXmxOkBp NInhbIpkcGGwZ3XzJIV5eHKnc4Ppc44heGG2dHXyckBw\iCpZX7ld{Bqdn[xbI\l[EBqdiClaH;s[ZN1\XKxbDDzfY51cGW|aYOsJJVxfGGtZTDhcoQh\W[obIX4 NFLKUJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{OyOlQzOid-MkOzNlY1OjJ:L3G+
THP1 NFrNWIdCeG:ydH;zbZMhSXO|YYm= NWjxe25wOSEQvF2= M2roZVI1KGh? M4TlWYlv\HWlZYRCpINmdGxiZHXheIg> NWLJeIZIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0NFA2OTlpPkKzOFAxPTF7PD;hQi=>
K562 MlXQRZBweHSxc3nzJGF{e2G7 MXSxJO69VQ>? NXrCN5p[OjRiaB?= NHzyRoRqdmS3Y3XzxsBk\WyuIHTlZZRp MoLLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2MEC1NVkoRjJ|NECwOVE6RC:jPh?=
Kazumi MlnrRZBweHSxc3nzJGF{e2G7 NFPJ[3cyKM7:TR?= Mly3NlQhcA>? M2HkN4lv\HWlZYRCpINmdGxiZHXheIg> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzRyMEWxPUc,OjN2MEC1NVk9N2F-
KG1 Mln5RZBweHSxc3nzJGF{e2G7 NYX1fJBtOSEQvF2= M1fUeVI1KGh? NYfkTGJ4cW6mdXPld:Kh[2WubDDk[YF1cA>? NFu2NpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SwNFUyQSd-MkO0NFA2OTl:L3G+
HL60 MoDBRZBweHSxc3nzJGF{e2G7 NEWzb5EyKM7:TR?= NX7iRo9zOjRiaB?= M1[4Tolv\HWlZYRCpINmdGxiZHXheIg> M{nSWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NECwOVE6Lz5{M{SwNFUyQTxxYU6=
HEL M1;BXWZ2dmO2aX;uJGF{e2G7 NFnKN5MxNjVizszN M1LaTVI1KGh? Mo\U[ZRp[W6xbB?= MX;k[YNz\WG|ZYOgeIhmKGWweont[UBi[3Srdnn0fUBw\iCKRFHDd{Bkdy22cnXheI1mdnRid3n0bEBkfXKldX3pci=> NF[2WoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{SzNFk2Pyd-MkO0N|A6PTd:L3G+
K562 MX3GeY5kfGmxbjDBd5NigQ>? MX:wMlUh|ryP MkjMNlQhcA>? NIjaWHpmfGijbn;s MUnk[YNz\WG|ZYOgeIhmKGWweont[UBi[3Srdnn0fUBw\iCKRFHDd{Bkdy22cnXheI1mdnRid3n0bEBkfXKldX3pci=> NX3SSIR{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0N|A6PTdpPkKzOFMxQTV5PD;hQi=>
LM8 Ml\CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\w[owxNjNizszN Mn3SNVIuQTZiaB?= NULGenpkTE2VTx?= M3\iWYlvcGmkaYTzJJRp\SClZXzsJIdzd3e2aDDjc{11emWjdH3lcpQhf2m2aDDt[ZRnd3KvaX6= NWn6dZpTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVE5OTdpPkKzOFUyQDF5PD;hQi=>
MG-63  MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjNOGUxNjNizszN NW\PWHZzOTJvOU[gbC=> M3m5N2ROW09? NXSwUYZpcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJINwNXS{ZXH0cYVvfCC5aYToJI1mfG[xcn3pci=> MkH6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NUG4NVcoRjJ|NEWxPFE4RC:jPh?=
H1299 M1zBXWZ2dmO2aX;uJGF{e2G7 MWGwMlUh|ryP NFqwZ2s1QCCq NWrhW3JmTE2VTx?= NUPReW42cW6qaXLpeJMhfGinIHnueoF{cX[nbnXzd:KhKGOxbXLpcoUhf2m2aDDzbYxq[mmwaX6= NYfEWFVmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OlE6PzVpPkKzOFYyQTd3PD;hQi=>
H1299 MUXGeY5kfGmxbjDBd5NigQ>? NIfxRZAxNjVizszN NYLVRWxVPDhiaB?= NHTUfJNFVVOR MXrpcohq[mm2czD0bIUhdWmpcnH0c5J6KHCxdHXueIlidCClb33ibY5mKHerdHigd4ltcWKrbnnu MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR4MUm3OUc,OjN2NkG5O|U9N2F-
H1299  MkLZSpVv[3Srb36gRZN{[Xl? NHe0TnIxNjN|4pETNeKhyrWP M3zHdFI1NzR6IHi= MVLEUXNQ M1frXYlv[3KnYYPld{BGNWOjZHjldolvKHC{b4TlbY4hdGW4ZXzzJIRwe2ViZHXw[Y5l\W62bIm= MnvnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2NkG5O|UoRjJ|NE[xPVc2RC:jPh?=
MCF7 M2\oS2Z2dmO2aX;uJGF{e2G7 NULGUIlSOC5yNT2yJO69VQ>? NHqxbVMzPCCq MWTy[YR2[2W|IITo[UBqdmS3Y4Tpc44hd2ZicEWzMYRmeGWwZHXueEBo\W6nczDifUBPfXSuaX6tNy=> NFuw[Ws9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S3NFU1OCd-MkO0O|A2PDB:L3G+
ARN8  NG\qcoZHfW6ldHnvckBCe3OjeR?= Mn65NE4xPS1{IN88US=> M3;HVFI1KGh? MWPy[YR2[2W|IITo[UBqdmS3Y4Tpc44hd2ZicEWzMYRmeGWwZHXueEBo\W6nczDifUBPfXSuaX6tNy=> Mlz2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2N{C1OFAoRjJ|NEewOVQxRC:jPh?=
U373 MmjkSpVv[3Srb36gRZN{[Xl? MkfBNE4zPS9yLkWvNUDPxE1? MWKyOEBp M3u4bYlv[3KnYYPld{BRWi2EIHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOWF7[WSF Mn3qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2N{SxO|EoRjJ|NEe0NVcyRC:jPh?=
HASMCs M2HLVWZ2dmO2aX;uJGF{e2G7 M{jiPVAuPTByIH7N MljPOkBl MVfkc5dvNXKnZ4XsZZRmeyC2aHWg[ZhxemW|c3nvckBw\iCKQVTDJIFv\CClZXzsJJZq[WKrbHn0fS=> M3HvU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUG4OFY4Lz5{M{WxPFQ3PzxxYU6=
MFE296 M4XTNmZ2dmO2aX;uJGF{e2G7 M4LvZVUxOCCwTR?= MV:1JIQ> MYnEUXNQ M13Wcolv\HWlZYOgUFFESU4EoHX4dJJme3Orb36gZ48ufHKnYYTt[Y51KHerdHigOU1CgmGF MmHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{C3OlkoRjJ|NUOwO|Y6RC:jPh?=
EN1 M3TLW2Z2dmO2aX;uJGF{e2G7 MXm1NFAhdk1? M4nxdlUh\A>? MXHEUXNQ NEmyS25qdmS3Y3XzJGwyS0GPwrDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFUuSXqjQx?= NY\pbnlpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1N|A4PjlpPkKzOVMxPzZ7PD;hQi=>
HEC1A MlHuSpVv[3Srb36gRZN{[Xl? NWHCbYcxPTByIH7N NYnIeWZtPSCm Mn3TSG1UVw>? MXLpcoR2[2W|IFyxR2FOyqCneIDy[ZN{cW:wIHPvMZRz\WG2bXXueEB4cXSqIEWtRZpiSw>? NWnm[YpCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1N|A4PjlpPkKzOVMxPzZ7PD;hQi=>
ECC1 NHHwelVHfW6ldHnvckBCe3OjeR?= MoDlOVAxKG6P NWDrdWVKPSCm Mn3xSG1UVw>? NHXt[3JqdmS3Y3XzJGwyS0GPwrDlfJBz\XO|aX;uJINwNXS{ZXH0cYVvfCC5aYToJFUuSXqjQx?= NFW5eVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNFc3QSd-MkO1N|A4Pjl:L3G+
AGS NVj0eWp{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonaNE4xOTVvMTFOwG0> M1PRWFczKGh? MmjEbY5lfWOnczDj[YxtKGSnYYToJINwdmOnboTyZZRqd25iZHXw[Y5l\W62bIm= NYfYTJpkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3OFUxOjRpPkKzO|Q2ODJ2PD;hQi=>
Huh7 MnvQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4TC[FEh|ryP MlzqNlQhcA>? MXLEUXNQ Mm\NdoVlfWOnczDhZo92fCB{MDWgeoli[mmuaYT5xsA> NUXme|lSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFM6OzNpPkKzOlQ{QTN|PD;hQi=>
Lovo NXnYNYp5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmT4NE4zNzFwMD:1MlAh|ryP NFrL[HMyOi9{ND:zOk81QCCq MnPybY5lfWOnczDj[YxtKGSnYYToJIlvKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= Mlr4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5N{CwNFAoRjJ|N{ewNFAxRC:jPh?=
HCT1 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDMNE4zNzFwMD:1MlAh|ryP NEfG[WUyOi9{ND:zOk81QCCq MYPpcoR2[2W|IHPlcIwh\GWjdHigbY4h[2:wY3XueJJifGmxbj2gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MkjLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5N{CwNFAoRjJ|N{ewNFAxRC:jPh?=
SUM190PT MoLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnfSOVAwOTByL{K1NEBvVQ>? MV[0PEBp NWq5RY5ITE2VTx?= NEf2ZZJqdmS3Y3XzJINmdGxiZHXheIgh[XRiNUCgcm0he2Wwc3n0bZZmdHl? NIi0bYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlY{QCd-MkO3PVI3Ozh:L3G+
SUM149PT NIC1fo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NELLd4wzNzdwNT:xNEDPxE1? MWO0PEBp NEfsfIxFVVOR NIL0RoNqdmS3Y3XzJINmdGxiZHXheIgh[XRiMjFOwG0hcW6|ZX7zbZRqfmWueR?= NF34b|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e5NlY{QCd-MkO3PVI3Ozh:L3G+
H1299 NYDWfZBzSXCxcITvd4l{KEG|c3H5 MYCwMlUwOSEQvF2= NFnDNIo1QCCq NHvKT2dmfGijbn;s NXf5VXZZcW6mdXPld{Bk\WyuIHHwc5B1d3Orcx?= M13J[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OE[3PVkyLz5{M{i2O|k6OTxxYU6=
A549 M13HWmFxd3C2b4Ppd{BCe3OjeR?= MX6wMlUwOSEQvF2= Ml3QOFghcA>? MnjB[ZRp[W6xbB?= MnHmbY5lfWOnczDj[YxtKGGyb4D0c5Nqew>? MmXYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6Nke5PVEoRjJ|OE[3PVkyRC:jPh?=
H1299 NVfQNXUzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGH6fFExNjJ3L{CuOU8yKM7:TR?= M1jEcFI1NzR6L{eyJIg> NE\DXoFmfGijbn;s NFX5SWhqdmirYnn0d{B1cGViY3XscEBoem:5dHigbY4h[2:wY3XueJJifGmxbjDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ Ml\6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6Nke5PVEoRjJ|OE[3PVkyRC:jPh?=
A549 NXSwOlB6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzET21NOC5{NT:wMlUwOSEQvF2= MoHHNlQwPDhxN{KgbC=> M4ntVIV1cGGwb3y= NWDa[2JkcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJIlvKGOxbnPlcpRz[XSrb36gZY5lKHSrbXWt[IVx\W6mZX70JI1idm6nch?= MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzh4N{m5NUc,OjN6Nke5PVE9N2F-
H1299 NV\oPGRUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGTLT2kyyqEQvF5CpC=> NYPPWmg{OjRxNEivO|IhcA>? MoSy[ZRp[W6xbB?= MUXpcoNz\WG|ZYOgZ4VtdCCmZXH0bEBi\nSncjCyOIg> MmrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MU[2NFkoRjJ|OUG2OlA6RC:jPh?=
PMNs Mkm1SpVv[3Srb36gRZN{[Xl? NEL6dIw{OCCwTR?= MYK0PEBp NXXJUnRJcW6qaXLpeJMh\W[oZYLvZ5l1d3OrczygTGRCSyCjY4Tpeol1gSCjbnSgVoFkKGGldHn2bZR6 NFrrdJU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m4PFYyPyd-MkO5PFg3OTd:L3G+
CA46 MmfNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXOzM|YwOTJxMkSvOFghdk1? NYfaN3NiPDhiaB?= NY\tN2RVcW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbjDheEAzPCCjbnSgOFghdk4EoB?= NH7BN289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC2OFk2OSd-MkSwOlQ6PTF:L3G+
HCT116 NETzd|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7UXGcxNjJizszN NVW5PZJZOTJiaB?= M4LLOoVvcGGwY3XzJINmdGxiYYDvdJRwe2m|IHnu[JVk\WRiYomgdoFlcWG2aX;u NEjOO289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGyNlI{OSd-MkSxNlIzOzF:L3G+
HLEB3 NEL0SIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HCTlAvPC9yLkig{txO NVPXW|ZlPDhiaB?= NHHFTWxFVVOR M1zHc4lv\HWlZYOgZ4VtdCCjcH;weI9{cXN? MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF3N{i3PEc,OjRzNUe4O|g9N2F-
SRA01/04  NYTFb|V5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFy5fGYxNjRxMD64JO69VQ>? MYq0PEBp NWr5TWJOTE2VTx?= M1XGU4lv\HWlZYOgZ4VtdCCjcH;weI9{cXN? NYHRPJBuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
HLEB3 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXPNE4zKM7:TR?= M3XWfVQ5KGh? Mnf1SG1UVw>? NX:1UHk5e3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC|dYDwdoV{e2mwZzD0bIUhWEl|Sz;Bb5QtKHB|OF3BVGsh[W6mIFXST|EwOiC|aXfuZYxqdmdicHH0bJdigXN? NUewcVU5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
SRA01/04  NV\nZllWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY\1RVVZOC5{IN88US=> NYL3c2tEPDhiaB?= M3[2[mROW09? MlfZd5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hfGi{b4XnbEB{fXCycnXzd4lv\yC2aHWgVGk{Uy:Da4SsJJA{QE2DUFugZY5lKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfZM> NF:xT4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1O|g4QCd-MkSxOVc5Pzh:L3G+
A2780 M4PBPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoOzNE4xPS1{IN88US=> NHG4cVMyOC9{ND:0PEBp NGjIT2tFVVOR M2LPeI1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NIPEVG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyN|gxOSd-MkSyNlM5ODF:L3G+
SKOV3 M2XZ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzqNE4xPS1{IN88US=> NXHzU4JwOTBxMkSvOFghcA>? MkfjSG1UVw>? NX[0c|FOdWWmaXH0[ZMh\3Kxd4ToJIFzemW|dDDpckBiKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= MlHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkO4NFEoRjJ2MkKzPFAyRC:jPh?=
Huh7  NXLidZZ3TnWwY4Tpc44hSXO|YYm= MViwMlEwOC53L{GuNEDPxE1? NXixeWdpOjSq NFy2PGp{fXCycnXzd4V{KFmWSFTDNkBo\W6nIHX4dJJme3Orb36= MoPXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{Nkm2O|IoRjJ2Mk[5OlczRC:jPh?=
hMSCs MYPGeY5kfGmxbjDBd5NigQ>? M3nUPFYvOjVibl2= MlnLNlQhcA>? M1rzUWROW09? NGDlb2t{fGGkaXzpfoV{KEirc4TvcoUhSWOndInsZZRqd25iYX7kJJRp\SCHeIDy[ZN{cW:wIH;mJHBtfXKrcH;0[Y51KEenbnXz M{HT[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{GyN|U3Lz5{NEOxNlM2PjxxYU6=
HT29  MkjMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\Kd3AyQDBibl2= NEPP[oU4OiCq NWX4O5lXUUN3MDDv[kAyQDBibl2= NXKxUXFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlgzPjVpPkK0N|Y5OjZ3PD;hQi=>
RPE M{nGemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\wbWU3OC5{L{CuOE8xNjhxMTFOwG0> NH7EWpkzPC92OD:3NkBp NILmd5lFVVOR NVn5V4t5cW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44h[nliY3XscEBkgWOuZTDhdpJme3R? NXi0XJA1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVY3ODJpPkK0OFU3PjB{PD;hQi=>
U87 GBM NEm2dGlHfW6ldHnvckBCe3OjeR?= Mn:zNVAxNzVyMDDOcS=> NEfNZ2I1QCCq NFjwVZIyODBnIHX0bIFvd2x? Ml3qTY5lfWOnczDT[Y5me2OnbnPlMWxqc2ViQXz0[ZJifGmxboOgbY4hVnWlbHXhdkBOd3KyaH;sc4d6 NFXxOIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES2OFg1OSd-MkS0OlQ5PDF:L3G+
U87 GBM NIe2OpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX6xNFAwOzByL{WwNEBvVQ>? NWK2VYVNPzJiaB?= Ml;PNVAxLSCndHjhco9t MX\y[YR2[2W|IH3lZY4h[2WubDDueY1j\XJiYomgN|EtKDV2LDDhcoQhPTkEoDW= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR4NEi0NUc,OjR2NkS4OFE9N2F-
ACP03 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1TCVVI2OC9|NUCvOVAxdk1? NH;0RXozPGh? MoT2[IVkemWjc3XzJINmdGxidnnhZoltcXS7IH;mJIFxeHKxeHntZZRmdHliN{FCpEUh[XRiMkWwxsBvVQ>? NX71c4x6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2Olg2PDdpPkK0OlY5PTR5PD;hQi=>
ACP02 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV[4cG0zOjVyL{O1NE82ODCwTR?= NWL0RYNxOjSq NEnPclFl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgc4Yh[XCycn;4bY1ifGWueTC3NOKhLSCjdDCyOVDDqG6P MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ4OEW0O{c,OjR4Nki1OFc9N2F-
HeLa NXHCR5NvTnWwY4Tpc44hSXO|YYm= NYjJSI43OW2P NWPHdlM5OC53aB?= MkLDZYJwdGm|aHXzJJRp\SC2b4ThcEBJTEGFIHHjeIl3cXS7 NGHWZZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEewO|Q4PCd-MkS3NFc1PzR:L3G+
HMEC-1  NF36TWlHfW6ldHnvckBCe3OjeR?= NEHXWnI{ODCwTR?= Mk\hNlRp NWntRnQ{cW6lcnXhd4V{KGW6cILld5Nqd25ib3dCpHZGT0[UM9MgcXJPSQ>? NWfBU2pFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVA3OzFpPkK0O|ExPjNzPD;hQi=>
HeLa  NHTRN3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPsZ5lEOs7:TR?= MVm0PIg> NVLWcZZF\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIFjd3W2IEK1KS=> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2NkGzOUc,OjR6NE[xN|U9N2F-
LNCaP M2L3Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3nNRVExOC1zMECwcm0> Mlv3NlRp M{DMPWlEPTBib3[gN|Axdk1? MmHDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NUS2OVgoRjJ2OEW0OlU5RC:jPh?=
PC3 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVTiUGV3OTByLUGwNFBvVQ>? MXyyOIg> M3rhTmlEPTBib3[gN|Axdk1? M{CzNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEW0OlU5Lz5{NEi1OFY2QDxxYU6=
CNE2 NEHtNnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TYVVExOC14MEDuUS=> NHjuSGgzPC92OD:3Nog> NUjxT4RncW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTD0bY1mNSCjbnSg[I9{\S2mZYDlcoRmdnRibXHucoVz MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4OUmwNUc,OjR7Nkm5NFE9N2F-
HeLa MWXGeY5kfGmxbjDBd5NigQ>? MkPtNlUxdk1? MkTXNVZp M{HkNmROW09? MULpcoNz\WG|ZYOgR3lROUFzIH3SUmEh\XiycnXzd4lwdsLi NWLvdmFnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVY3QDhpPkK1NVE3Pjh6PD;hQi=>
A549 MmDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFywUogxNjBzLUCuPe69VQ>? NXj2NohqPzKq M2mxVmROW09? M3W1RWlEPTBib3[gNE4xPs7:TR?= M1faflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
SKOV-3 NYLoUIlGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTjTXcxNjBzLUCuPe69VQ>? NGniR2Y4Omh? MVnEUXNQ NVvQVZk4UUN3MDDv[kAxNjN{zszN NX7YfZFURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOlk1QTFpPkK1NVY6PDlzPD;hQi=>
A549 M2LMbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHPSeXQxNjFvMd88US=> Mo\qOFhp NE\Id|hFVVOR M2L4TGlEPTBib3[gNE4zQM7:TR?= NIPoUpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2PVQ6OSd-MkWxOlk1QTF:L3G+
SKOV-3 M{nzbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\jNE4yNTIQvF2= NVjJZ2h7PDiq MWrEUXNQ Mn7iTWM2OCCxZjCwMlfPxE1? M3izT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
A549 NV3FWVNwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NInOSHIyNTFyzszN MkOyNlRp NXLZZlQ5TE2VTx?= NETpb|ZKSzVyIH;mJFMvOs7:TR?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
SKOV-3 NH;6dYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\yeIgyNTFyzszN M4jqU|I1cA>? MV7EUXNQ MWLJR|UxKG:oIEWuOu69VQ>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
MCF7 Mmm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3TYcVAuPjBybl2= M3nydlI1cA>? M2C5WGROW09? NGTyOVJKSzVyIH;mJFc2dk1? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF7MkeyNUc,OjVzOUK3NlE9N2F-
MDA-MB-231 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrkNE03ODCwTR?= NWOwe|lsOjSq NFPzOndFVVOR NUntWnViUUN3MDDv[kAyODCwTR?= NF74fms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG5NlczOSd-MkWxPVI4OjF:L3G+
A431 MUDGeY5kfGmxbjDBd5NigQ>? MnzNOVBvVQ>? NWLCd5dqOi94L{GyM|I1cA>? NG\mcGpFVVOR MXLhZ5RqfmG2ZYOgdFIyKGGwZDDpcohq[mm2czDBWGY{KGW6cILld5Nqd25? NUHuNIh4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzO|ExPjlpPkK1N|cyODZ7PD;hQi=>
A431 NFnxemdCeG:ydH;zbZMhSXO|YYm= NUn2RpQ6Oi9zMD:1NE8yODCwTR?= M1PEUFQ5cA>? NV3NWGdDTE2VTx?= NIrTcopqdmirYnn0d{B1cGViY3XscEBoem:5dHi= M1rmSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{exNFY6Lz5{NUO3NVA3QTxxYU6=
PC3  NELjeWVHfW6ldHnvckBCe3OjeR?= NFfjR3cxNjIQvF2= MnvkOFhp NH3OXm1FVVOR M33Xbolv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ? NV;mUplWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|Q6QTdpPkK1OFM1QTl5PD;hQi=>
SW480  MnzaSpVv[3Srb36gRZN{[Xl? NF32VIMxNjIQvF2= NEfOeG41QGh? NVHZcVN5TE2VTx?= M3:5eolv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ? NHrSSHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzOFk6Pyd-MkW0N|Q6QTd:L3G+
PC3  NXq1bVhHTnWwY4Tpc44hSXO|YYm= MVewMlHPxE1? M16wcVQ5cA>? NWPFSXR[TE2VTx?= NGXG[ndifHSnboXheIV{KGmwdnHzbY9vKGGwZDDtbYdz[XSrb36= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR|NEm5O{c,OjV2M{S5PVc9N2F-
PC3  NIrHfZVHfW6ldHnvckBCe3OjeR?= M2DOWlAvOc7:TR?= M3qyXFQ5cA>? NX\hZXlsTE2VTx?= NHjXcYlz\X[ncoPld{BGVVR? M2XieFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
SW480  M1jvU2Z2dmO2aX;uJGF{e2G7 MoLXNE4y|ryP Mn[2OFhp NHvtb3VFVVOR MX\heJRmdnWjdHXzJIlvfmG|aX;uJIFv\CCvaXfyZZRqd25? M4\NW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
SW480  NEHLTFFHfW6ldHnvckBCe3OjeR?= NVfoTW55OC5zzszN MmHzOFhp NUOwT3R6TE2VTx?= MVjy[ZZmenOnczDFUXQ> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR|NEm5O{c,OjV2M{S5PVc9N2F-
MEFs MVPGeY5kfGmxbjDBd5NigQ>? NHXM[Ho2|ryP Mn\3NVZp M1fGVolv[3KnYYPld{B1cGViRWDFR{BifHSjY3jt[Y51NCCWaYKg[IVtcX[ncomgZY5lKHSqZTDl[oZq[2mnbnP5JI9nKHCnZHXzeIFtKG[xcn3heIlwdg>? NUexeYtlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PFI3OzRpPkK1OFgzPjN2PD;hQi=>
TE13 MV;BdI9xfG:|aYOgRZN{[Xl? NXP2dnd6OC5|zszN MV:yOIg> NWXrS5VZcW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbh?= M2W5SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUe5OlY2Lz5{NUW3PVY3PTxxYU6=
TE13 M1vMZWZ2dmO2aX;uJGF{e2G7 NWXXRWY{OC5|zszN MnTWNlRp MWr1dE1z\We3bHH0[ZMhWkGVU1[1RUBt\X[nbB?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV5OU[2OUc,OjV3N{m2OlU9N2F-
HEK 293 NGK4SnpHfW6ldHnvckBCe3OjeR?= MVKwMlfPxE1? MYWyOIg> MnHQ[ZRp[W6xbB?= NWTYXnY6\W6qYX7j[ZMhTU6jQzDhZ4V1gWyjdHnvckBidmRiaX7jdoVie2W|IFXOZWMh[WK3bnThcoNmKGmwIITo[UB1d3SjbDDj[YxtKGy7c3H0[UBidmRiYYSgeIhmKGOnbHygd5Vz\mGlZR?= NWTC[|E1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3PFcxPzlpPkK1O|g4ODd7PD;hQi=>
MDA-MB-231 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUXJUWZzOjVvNECwJI5O NVHMd4xyPDhiaB?= NHTNPGtKSzVyIH;mJFI3Oy5{bl5CpC=> NFfTTlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C1OVE6QCd-MkOwOVUyQTh:L3G+
MCF-7  NFvUS3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYKyOU01ODBibl2= MlrVOFghcA>? MoHDTWM2OCCxZjCyNlAvPG6P NXLyeHE1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOVUyQThpPkKzNFU2OTl6PD;hQi=>
ECC-1  MlXhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUfX[ppxOTByIH7N NGDzbI8zPCCq MWHleIhidm:u NGTibHJqdmO{ZXHz[ZMhfGinIIDyc5BwenSrb36gc4Yh[XCxcITveIlkKG63Y3zlbUB1dyB|NTW= NEjLWI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CyPFgxOyd-MkOwNlg5ODN:L3G+
HEC-1A M2XVPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTXdnJHOTByIH7N MonyNlQhcA>? M3vIVIV1cGGwb3y= M1vWOYlv[3KnYYPld{B1cGVicILvdI9zfGmxbjDv[kBieG:ydH;0bYMhdnWlbHXpJJRwKDN7JR?= MnTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMki4NFMoRjJ|MEK4PFA{RC:jPh?=
NHAC-kn MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLqc5BZOTBxMUCwM|UxOCCwTR?= M3jpWVEzKGh? MXTEUXNQ NWXvTIhtUUN3MDDv[kA2ODCwTR?= MlXJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMUe4O|EoRjJ|MEG3PFcyRC:jPh?=
A549 NYnaZ29kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkLvNlUxKG6P MYO2MVczKGh? MmG5SG1UVw>? MkLNZ4F2e2W|IHGg[5Jm[XSncjDpcohq[mm2b4L5JIVn\mWldDDjc41jcW6nIIfpeIghXFiWIH;yJIVzdG:2aX7pZi=> MlvPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7OUS3PFAoRjJ{OUm0O|gxRC:jPh?=
MG-63 Ml\vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYC0fpZtOzByIH7N M4PtcVEzKGh? M{jNdGROW09? MlW0bY5pcWKrdIOgeIhmKGOnbHyg[5Jwf3SqIITvJFg3LQ>? NEXSNJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke5PVM{QCd-MkK3PVk{Ozh:L3G+
MG-63 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVOzNFAhdk1? M4HVSlI1KGh? NVTpXFVQTE2VTx?= NVjHeFhQcW6qaXLpeJMhfGinIHPlcIwh\3Kxd4ToJJRwKDZ5JR?= M2\M[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{m5N|M5Lz5{Mke5PVM{QDxxYU6=
MG-63 MljXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWmzNFAhdk1? MYW0PEBp MlHDSG1UVw>? NHrkPJJqdmirYnn0d{B1cGViY3XscEBoem:5dHigeI8hPTZn NFPKUGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mke5PVM{QCd-MkK3PVk{Ozh:L3G+
HL60  MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LOS|E2OC1|NUCgcm0> NYDoeIpjOjRiaB?= Mkn1bY5kemWjc3XzJINmdGxiYYDvdJRwe2m|wrD3bIVvKGOxbnPlcpRz[XSrb37zJIhq\2incjD0bIFvKDJ3MDDuUS=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd3M{ezPUc,OjJ5NUO3N|k9N2F-
U937 M3LLeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXy1RZNyOTVyLUO1NEBvVQ>? NGDaPIMzPCCq NFjL[3FqdmO{ZXHz[ZMh[2WubDDhdI9xfG:|aYRCpJdp\W5iY3;uZ4VvfHKjdHnvcpMhcGmpaHXyJJRp[W5iMkWwJI5O MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd3M{ezPUc,OjJ5NUO3N|k9N2F-
SCC-6 NUXpd5ZnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLVUlEzODBvM{KwNEBvVQ>? M4rqZ|EzNzJ2L{S4JIg> MXHEUXNQ MoLSbY5pcWKrdIOgeIhmKHC{b3zp[oVz[XSrb36gc4YhW0OFLU[gZ4VtdHNiaX6gZUBld3OnLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{ NIHOTJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkW1NlMzOSd-MkK1OVI{OjF:L3G+
U87  NX3l[|NWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXexNFAuOzByIH7n MkL1NlQhcA>? MX;pcohq[mm2czD0bIUh[2WubDDndo94fGhidH:gO|ImKCCjdDCyNFBv\w>? M{HsUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkewPFQ6Lz5{MkK3NFg1QTxxYU6=
K562 NInXWYdHfW6ldHnvckBCe3OjeR?= M2nwUVEh|ryP MmjBNVIhcA>? NInLPYZFVVOR MlPY[Y5p[W6lZYOgeIhmKGW6cILld5Nqd25ib3[gVnVPYDNiaX7keYNm\CCkeTC1MYF7[S2FZGK= MoHIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzN{mxPVgoRjJ{MUe5NVk5RC:jPh?=
Reh MmD4SpVv[3Srb36gRZN{[Xl? NWS0cZlyOC5|L{Gg{txO M1W3RVEzKGh? NYTjRoZqTE2VTx?= MkfL[Y5p[W6lZYOgeIhmKGW6cILld5Nqd25ib3[gVnVPYDNiaX7keYNm\CCkeTC1MYF7[S2FZGK= MmjQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJzN{mxPVgoRjJ{MUe5NVk5RC:jPh?=
Mv1Lu NF;rOoxHfW6ldHnvckBie3OjeR?= M{TvXlE5KGi{cx?= Mm\zTY5lfWO2aX;uJI9nKHerbHSteJlx\SCyMkGgdJJwdW:2ZYKgZYN1cX[jdHnvckBqdiCvaX7rJG13OUy3IHPlcIx{KGGodHXyJFE5KGi{czDifUBtfWOrZnXyZZNmKGG|c3H5JJJmdGG2aY\lJJRwKGKjc3HsJIxmfmWuLDDFR|ExODBiPTCwMlE6KM7:TT6= MlvyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OEGyN|IoRjF5OEixNlMzRC:jPh?=
HeLa NEDPfYtHfW6ldHnvckBie3OjeR?= MlWxNlQhcHK| MYTJcoR2[3Srb36gc4YheDJzIHX4dJJme3Orb36gbY4hcHWvYX6gTIVN[SClZXzsd{Bi\nSncjCyOEBpenNuIFXDNVAxOCB;IECuNFEyPSEQvF2u NUD0NG82RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPVc5OjZpPkG4N|k4QDJ4PD;hQi=>
Mv1Lu NXzwdYRCTnWwY4Tpc44h[XO|YYm= NXWzb|RoOjRiaILz NWrsPJZMUW6lcnXhd4UhcW5iaIXtZY4hf2muZDD0fZBmKHB{MTDwdo91\WmwIHX4dJJme3Orb36gbY4hdWmwazDNelFNfSClZXzsd{Bi\nSncjCyOEBpenNiYomgdFIyKHC{b33veIVzKGG|c3H5MEBGSzFyMECgQUAxNjB{IN88UU4> MnLJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7OUe1O|goRjJ2OUm3OVc5RC:jPh?=
HCT 116 MWTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MVLJckB3cXS{bzDhcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKGOxbH;uJINidmOncjDIR3QhOTF4IHPlcIx{KGSndHXycYlv\WRiYomgUW1VKGG|c3H5MEBGSzVyIE2gNUDPxE1w NGPyPYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5O|QyOyd-MUG1PVc1OTN:L3G+
T24 NG\WUJRHfW6ldHnvckBie3OjeR?= MlHzR49v[2WwdILheIlwdiCxZjDjc41xd3WwZDDy[ZF2cXKnZDDmc5Ih[WOndInsZZRqd25ib3[gbIl{fG:wZT20JIlvKGi3bXHuJHQzPCClYX7j[ZIh[2WubIOsJGVEPTBiPTCxJO69VS5? NEjFdpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUW5O|QyOyd-MUG1PVc1OTN:L3G+
T24 NHLoWHdHfW6ldHnvckBie3OjeR?= NGHLS2lKdmirYnn0bY9vKG:oIHHj[ZR6dGG2aX;uJI9nKGirc4TvcoUuPCCrbjDoeY1idiCWLUK0JINidmOncjDj[YxteyxiRVO1NEA:KDFizszNMi=> NVHDfHhzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS2NVM{OTJpPkG0OlE{OzF{PD;hQi=>
T24 MkKzSpVv[3Srb36gZZN{[Xl? NGTocGdKdmirYnn0bY9vKG:oIHHj[ZR6dGG2aX;uJI9nKGirc4TvcoUuPCCrbjDoeY1idiCWLUK0JINidmOncjDj[YxteyxiRVO1NEA:KDRizszNMi=> M3Ty[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NkGzN|EzLz5zNE[xN|MyOjxxYU6=
T24 M4H5PGZ2dmO2aX;uJIF{e2G7 NIjVfHVKdmS3Y4Tpc44hd2ZiYXzwbIEhfHWkdXzpckBi[2W2eXzheIlwdiCrbjDoeY1idiCWMkSgZ4VtdHNuIFXDOVAhRSByLkCxJO69VS5? NEnoOYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGxNVQ3Pid-MUmxNVE1PjZ:L3G+
T24 NEXkd|FHfW6ldHnvckBie3OjeR?= Mm\ETY5lfWO2aX;uJI9nKGirc4TvcoUhUDNiYXPleJlt[XSrb36gbY4hcHWvYX6gWFI1KGOnbHzzMEBGSzVyIE2gNE4xOSEQvF2u NE\KNGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUGxNVQ3Pid-MUmxNVE1PjZ:L3G+
HepG2 MljRSpVv[3Srb36gZZN{[Xl? Ml3xWZAuemWpdXzheIlwdiCxZjD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHlib3[gbJVu[W5iQ1zBMVEheHKxbX;0[ZIhemWpaX;uJE0yODV3IITvJE03OmKyIITyZY5{\mWldHXkJIlvKEincFeyJINmdGy|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwM{ig{txONg>? MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3NkGwNkc,OjV3NU[xNFI9N2F-
HeLa NVfRblNTTnWwY4Tpc44h[XO|YYm= NYPacW91UW6qaXLpeIlwdiCxZjDISGFEPiCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZidIXieYxqdiCmZXHj[ZR6dGG2aX;uJIlv[3WkYYTl[EBnd3Jib4\ldo5q\2i2IHL5JINGVEmVQTygSWM2OCB;IECuNFA2OiEQvF2u MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZzMUmxPUc,OjZ4MUG5NVk9N2F-
HL60 MYHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? M2XaZlI1KGi{cx?= NXvF[3NLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGdKPTBiPTCwMlU6KM7:TT6= NI\TNIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS1OFkyQCd-Mkm0OVQ6OTh:L3G+
MDA-MB-435 NUXwfY02SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NYLlcVdkOjRiaILz NWf3PXJGSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNEO1JINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCPVGSgZZN{[XluIFfJOVAhRSBzLke4JO69VS5? NG\BTVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS1OFkyQCd-Mkm0OVQ6OTh:L3G+
K562 M4O2[2Z2dmO2aX;uJIF{e2G7 MlSwTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JJBienSrYXzsfUBxfXKrZnnl[EBpcXO2b37lJIRm[WOndInsZZNmKG:oIHj1cYFvKGyndXvlcYliKEt3NkKgZ4VtdHNwLDDJR|UxKD1iMD6wNFQ3KM7:TT6= NET5RWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMESyOVEyOCd-MUC0NlUyOTB:L3G+
A20 NE\id|ZHfW6ldHnvckBie3OjeR?= NX3UTGc5UW5idnn0do8h\m:{IHHueIkuUESDQ{GgLGhqe3SxbnWg[IVi[2W2eXzhd4UhOSliYXP0bZZqfHliaX6gcY92e2ViQUKwJINmdGy|LDDJR|UxKD1iMD6wNFIh|ryPLh?= NWDLbWdsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG5OlA1QDlpPkGxPVYxPDh7PD;hQi=>
K 562 Ml;PSpVv[3Srb36gZZN{[Xl? M3iwcmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBJcXO2b37lJIRm[WOndInsZZNmKCiKRFHDLUBqdiCNIEW2NkBmenm2aILvcIV2c2WvaXGgZ4VtdHNuIFnDOVAhRSByLkCwN|Qh|ryPLh?= MkHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3OUK0O|MoRjF2NUmyOFc{RC:jPh?=
friend cell MoHXSpVv[3Srb36gZZN{[Xl? MWnJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCocnnlcoQh[2WubIOgdJJwdGmoZYLheIlwdixiSVO1NEA:KDBwMESg{txONg>? NUfjVWZYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS2NVM{OTJpPkG0OlE{OzF{PD;hQi=>
SNU-16 MX;GeY5kfGmxbjDhd5NigQ>? NUDnc5BRUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEirc4TvcoUh\GWjY3X0fYxie2ViKFjERWMqKG[{b32gV25WNTF4IDjoeY1idiCpYYP0dolkKGGmZX7vZ4Fz[2mwb33hLUBk\WyuczygTWM2OCB;IECuNFU{OjlizszNMi=> MnPMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR4NkeyNlcoRjF2Nk[3NlI4RC:jPh?=
melanoma B16/BL6 MWPGeY5kfGmxbjDhd5NigQ>? NV7hNlU5UW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KGirc4TvcoUh\GWjY3X0fYxie2W|IDjISGFEOSlicILldIFz\WRiZoLvcUBud3W|ZTDt[Yxidm:vYTDCNVYwSkx4IHPlcIx{NCCLQ{WwJF0hOC5yMUmg{txONg>? NIe5b3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUGwPVYzPid-MUWxNFk3OjZ:L3G+
melanoma B16/BL6 MnHwSpVv[3Srb36gZZN{[Xl? M4LvbmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKCiKRFHDOEkheHKncHHy[YQh\nKxbTDtc5V{\SCvZXzhco9u[SCEMU[vRmw3KGOnbHzzMEBKSzVyIE2gNE4xOiEQvF2u MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
melanoma B16/BL6 NYj5T|YxTnWwY4Tpc44h[XO|YYm= MmnHTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIhqe3SxbnWg[IVi[2W2eXzhd4UhPiCycnXwZZJm\CCocn;tJI1wfXOnIH3lcIFvd22jIFKxOk9DVDZiY3XscJMtKEmFNUCgQUAxNjB{ODFOwG0v MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
melanoma B16/BL6 NVTFZY1YTnWwY4Tpc44h[XO|YYm= NGTQR5hKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcGm|dH;u[UBl\WGlZYT5cIF{\SB6IIDy[ZBiemWmIH\yc40hdW:3c3WgcYVt[W6xbXGgRlE3N0KONjDj[YxteyxiSVO1NEA:KDBwMESg{txONg>? NGnnTnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUGwPVYzPid-MUWxNFk3OjZ:L3G+
293T NXLaNW5JTnWwY4Tpc44h[XO|YYm= M{XH[mlvcGmkaYTpc44hd2ZiaIXtZY4hcGm|dH;u[UBl\WGlZYT5cIF{\SB2IIDy[ZBiemWmIH\yc40hOjl|VDDj[YxteyxiSVO1NEA:KDBwMEKg{txONg>? MkLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyNlQoRjF3NEW0NlI1RC:jPh?=
293T Ml;USpVv[3Srb36gZZN{[Xl? MY\Jcohq[mm2aX;uJI9nKGi3bXHuJIhqe3SxbnWg[IVi[2W2eXzhd4UhOSCycnXwZZJm\CCocn;tJFI6O1RiY3XscJMtKEmFNUCgQUAxNjB{IN88UU4> MkS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyNlQoRjF3NEW0NlI1RC:jPh?=
293T NXPDUGR[TnWwY4Tpc44h[XO|YYm= NVvBTnVxUW6qaXLpeIlwdiCxZjDoeY1idiCqaYP0c45mKGSnYXPleJlt[XOnIE[gdJJmeGG{ZXSg[pJwdSB{OUPUJINmdGy|LDDJR|UxKD1iMD6wNkDPxE1w NGrXN2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUS1OFIzPCd-MUW0OVQzOjR:L3G+
293T M3fXVGZ2dmO2aX;uJIF{e2G7 MWPJcohq[mm2aX;uJI9nKGi3bXHuJIhqe3SxbnWg[IVi[2W2eXzhd4UhQCCycnXwZZJm\CCocn;tJFI6O1RiY3XscJMtKEmFNUCgQUAxNjB2IN88UU4> M3;pXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEW0NlI1Lz5zNUS1OFIzPDxxYU6=
HeLa NWO1[pBMTnWwY4Tpc44h[XO|YYm= M3HG[2lvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKGirc4TvcoUh\GWjY3X0fYxie2ViKFjERWMqKG[{b32gTIVN[SClZXzsd{B4cXSqIIP1ZpN1emG2ZTC1ZUwhUUN3MDC9JFAvODV2NTFOwG0v MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR3NkK2O{c,OTV2NU[yOlc9N2F-
HCT116 M1\jUmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NWq1Uo1rSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBJS1RzMU[gZ4VtdHNuIFnDOVAhRSByLkCwNVMh|ryPLh?= M3;YPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUC0PFkxLz5zNkmwOFg6ODxxYU6=
H1299 MnLlRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MkLDRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDINVI6QSClZXzsd{whUUN3MDC9JFAvOSEQvF2u MlrvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MES4PVAoRjF4OUC0PFkxRC:jPh?=
H661 MmewRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MmOzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDIOlYyKGOnbHzzMEBKSzVyIE2gNE4{KM7:TT6= M1;FS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUC0PFkxLz5zNkmwOFg6ODxxYU6=
H661 MYfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MXy0PEBpenN? NXnhUlRRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBJPjZzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUCgQUAxNjB6NTFOwG0v M{T5VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkK0O|c{Lz5zN{[yOFc4OzxxYU6=
WiDr NELlNmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NGDKXIxKdmirYnn0bY9vKG:oIGPBVFE{OCCvZXTpZZRm\CClZXzsJIdzd3e2aDDpckBpfW2jbjDXbWRzKGOnbHzzMEBKSzVyIE2gNE42Pzd4IN88UU4> M33QUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkSzNVEzLz5zN{[0N|EyOjxxYU6=
U251 NEDHfVRHfW6ldHnvckBie3OjeR?= M1\RUGlvcGmkaYTpc44hd2ZiU1HQNVMxKGmwIG\FS2Yue3SrbYXsZZRm\CCqdX3hckBWOjVzIHPlcIx{KGK7IGDMRXAhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwNkGxNkDPxE1w NUfINItURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OFMyOTJpPkG3OlQ{OTF{PD;hQi=>
293T MlX3SpVv[3Srb36gZZN{[Xl? MVPJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNzIHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yMEG5JO69VS5? NGL6dlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i4NVI{Oid-MUe4PFEzOzJ:L3G+
293T MoL0SpVv[3Srb36gZZN{[Xl? MUTJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN2IHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yMEKg{txONg>? M1HMcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEixNlMzLz5zN{i4NVI{OjxxYU6=
293T NHS3TnRHfW6ldHnvckBie3OjeR?= MnPZTY5pcWKrdHnvckBw\iCvb4Xz[UBJTEGFNjDlfJBz\XO|ZXSgbY4hOjl|VDDj[YxteyxiSVO1NEA:KDBwMECyPEDPxE1w Ml7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OEGyN|IoRjF5OEixNlMzRC:jPh?=
H661 NIjvVFdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M3vMOVQ5KGi{cx?= MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFi2OlEh[2WubIOgZYZ1\XJiNEigbJJ{NCCLQ{WwJF0hOC5yOEWg{txONg>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh7N{iyOEc,OTd6OUe4NlQ9N2F-
293T NHnWfmJHfW6ldHnvckBie3OjeR?= NE\UbpFKdmirYnn0bY9vKG:oIFjERWMyKGW6cILld5Nm\CCrbjCyPVNVKGOnbHzzMEBKSzVyIE2gNE4yQSEQvF2u NIrS[5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mwNFkyOSd-MUe5NFA6OTF:L3G+
293T MX3GeY5kfGmxbjDhd5NigQ>? MVXJcohq[mm2aX;uJI9nKEiGQVO0JIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy|LDDJR|UxKD1iMD6yJO69VS5? NXf2WmNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
293T Mn\tSpVv[3Srb36gZZN{[Xl? M1zsdmlvcGmkaYTpc44hd2ZiSFTBR|Yh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOsJGlEPTBiPTCwMlI5KM7:TT6= NILlVVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mwNFkyOSd-MUe5NFA6OTF:L3G+
NIH3T3 M1zwe2Z2dmO2aX;uJIF{e2G7 MnLJTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDNUBmgHC{ZYPz[YQhcW5iTlnIN3Q{KGOnbHzzMEBKSzVyIE2gNE4xOjFizszNMi=> NUXpR4E3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5Nlk4QThpPkG3PVI6Pzl6PD;hQi=>
human 293 Mk\1SpVv[3Srb36gZZN{[Xl? NFq2bpNKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWM1KGW6cILld5Nm\CCrbjCyPVMh[2WubIOsJGlEPTBiPTCwMlA{PCEQvF2u Mkf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7Mkm3PVgoRjF5OUK5O|k5RC:jPh?=
mouse 293 M2D6bmZ2dmO2aX;uJIF{e2G7 Mn\ZTY5pcWKrdHnvckBw\iCvb4Xz[UBz\WOxbXLpcoFvfCCKRFHDOkBmgHC{ZYPz[YQhcW5iMkmzJINmdGy|LDDJR|UxKD1iMD6wPFEh|ryPLh?= NGLoeoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{myPVc6QCd-MUe5Nlk4QTh:L3G+
SKBR3 NYm1[YxpSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MYPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFONQmKzJINmdGy|LDDJR|UxKD1iMD6wNkDPxE1w NUfDSpM3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
MM96L NGfzUoNHfW6ldHnvckBie3OjeR?= NXv3RYJFUW6qaXLpeIlwdiCxZjDoeY1idiCPTUm2UEBk\WyuczygTWM2OCB;IECuNFMh|ryPLh?= Mlz0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEe1OVQoRjF6MkS3OVU1RC:jPh?=
SNU16 M4iwZWZ2dmO2aX;uJIF{e2G7 MYTJcohq[mm2aX;uJI9nKEiGQVOg[pJwdSCqdX3hckBUVlVzNjDj[YxteyxiSVO1NEA:KDBwMEWzJO69VS5? NYCxfHF3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
A549 MYnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MlLKRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|LDDJR|UxKD1iMD6wPEDPxE1w NED6bYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O|U2PCd-MUiyOFc2PTR:L3G+
MKN45 NVO5enA{SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MnrYRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPS160OUBk\WyuczygTWM2OCB;IECuNUDPxE1w MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
SQ20B MV\GeY5kfGmxbjDhd5NigQ>? NGj1e45KdmirYnn0bY9vKG:oIHj1cYFvKFOTMkDCJINmdGy|LDDJR|UxKD1iMD6yJO69VS5? NETCTIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O|U2PCd-MUiyOFc2PTR:L3G+
neonatal foreskin fibroblasts Mmm1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MVnHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDu[Y9v[XSjbDDmc5Jme2urbjDmbYJzd2KuYYP0d{Bk\WyuczygTWM2OCB;IECuNkDPxE1w MlnlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEe1OVQoRjF6MkS3OVU1RC:jPh?=
HeLa NYDufWE{TnWwY4Tpc44h[XO|YYm= M3XYV2lvcGmkaYTpc44hd2ZiSFTBR{Bnem:vIHj1cYFvKEinTHGgZ4VtdHNuIFnDOVAhRSByLkOg{txONg>? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MM96L/NFF NE\OeWJHfW6ldHnvckBie3OjeR?= MXrT[Yxm[3Srdnn0fUBqdmSneDygdoF1cW9ib3[gTWM2OCCob4KgbJVu[W5iTV25Omwh[2WubIOgeI8hUUN3MDDmc5IhcHWvYX6gUmZHKGOnbHzzMEBKSzVyIE2gOk44KM7:TT6= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
293T M4rtV2Z2dmO2aX;uJIF{e2G7 MlPHTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTDlfJBz\XO|ZXSgbY4hOjl|VDDj[YxteyxiSVO1NEA:KDBwMEO2OkDPxE1w NG\3eXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5O|gzPid-MUizPVc5OjZ:L3G+
293T MUfGeY5kfGmxbjDhd5NigQ>? NUeye3FFUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNFYzQSEQvF2u Ml\sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|OUe4NlYoRjF6M{m3PFI3RC:jPh?=
293T MUnGeY5kfGmxbjDhd5NigQ>? MXvJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN4IHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yOEOzJO69VS5? MmPBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|OUe4NlYoRjF6M{m3PFI3RC:jPh?=
HeLa NV3Zd29XTnWwY4Tpc44h[XO|YYm= MUDJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNiaX6gTIVN[SClZXzsd{BjgSCobH;1dkBl\SCueYOgZZN{[XluIFnDOVAhRSByLkCwOUDPxE1w Mk\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh|OUe4NlcoRjF6M{m3PFI4RC:jPh?=
S2 MlPaSpVv[3Srb36gZZN{[Xl? MUnJcohq[mm2aX;uJI9nKFCuYYPtc4RqfW1iZnHsZ4lx[XK3bTDISGFEOSCneIDy[ZN{\WRiaX6gSJJwe2:yaHnsZUBu\Wyjbn;nZZN1\XJiU{KgZ4VtdHNuIFnDOVAhRSByLkCwNFYh|ryPLh?= M13oNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{G3OFUxLz5zOUOxO|Q2ODxxYU6=
NCI-H661 NWXKSoo4SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M3jxS|Q5KGi{cx?= NW[2TGhkSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDZ4MTDj[YxteyCjZoTldkA1QCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwJF0hOC5zIN88UU4> MmjOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl|OEW2NFAoRjF7M{i1OlAxRC:jPh?=
NIH3T3 M1vrSGZ2dmO2aX;uJIF{e2G7 MY\Jcohq[mm2aX;uJI9nKEiGQVOxJIlvKGi3bXHuJG5KUDOWMzDj[YxteyCkeTDyZYRqd22ndILpZ{BpcXO2b37lJIRm[WOndInsZZRqd25iYYPzZZktKEmFNUCgQUAxNjByNjFOwG0v MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR3N{[1PUc,OTl2NUe2OVk9N2F-
HeLa MWXGeY5kfGmxbjDhd5NigQ>? MknNTY5pcWKrdHnvckBw\iCKRFHDJIZzd21iaIXtZY4hUGWOYTDj[YxteyCkeTDmcJVwem:pZX7pZ{Bmdnq7bXWgZZN{[XluIFnDOVAhRSByLkOg{txONg>? NUnMU4c2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OVc3PTlpPkG5OFU4PjV7PD;hQi=>
ML1 NX36XIViSW62aYT1cY9zKGG|c3H5 MkSzRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgcY92e2ViTVyxJINmdGy|LDDJR|UxKD1iMD6yOUDPxE1w Mm\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3M{S1N|QoRjF7NUO0OVM1RC:jPh?=
HCT116 MVHDfZRwfG:6aXPpeJkh[XO|YYm= NH\VUW4{KGSjeYO= MXPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JHdUXC1zIHHzd4F6NCCLQ{WwJF0hOC56IN88UU4> M4jKSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWyNlI3Lz5{MES1NlIzPjxxYU6=
HCT116 NW\QbZhST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NIrTdFg1QCCqcoO= NXTPOYFRT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hUEOWMUG2JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XluIFnDOVAhRSByLkC0N{DPxE1w NWP5d|dvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwO|MyPjBpPkKxNFc{OTZyPD;hQi=>
H460 NX:4dYpuT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVi0PEBpenN? NEXSeXVIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJPDZyIHPlcIx{KGGodHXyJFQ5KGi{czDifUBUWkJiYYPzZZktKEmFNUCgQUAxNjFizszNMi=> MlHlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyN{OxOlAoRjJzMEezNVYxRC:jPh?=
DMS53 NFn4bGJEgXSxdH;4bYNqfHliYYPzZZk> MV6yJIRigXN? Mon1R5l1d3O2YYTpZ{Bi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFTNV|U{KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gZ4VtdCCycn;sbYZmemG2aX;uJIFnfGW{IEKg[IF6eyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlAzPSEQvF2u MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTVyNEKxOEc,OjF3MESyNVQ9N2F-
HeLa M4fBRmZ2dmO2aX;uJIF{e2G7 M2T4[|IxKG2rboO= M37IXmlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBJ\UyjIHPlcIx{KHW|aX7nJGFkNUG{Zz3HcJkuVHm|KFHjLU1CVUNiYYOg[ox2d3Knc3PlcpQhe3Wkc4TyZZRmKGGodHXyJFIxKG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOC5yMEOg{txONg>? M1HsbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkKxPFg{Lz5{MU[yNVg5OzxxYU6=
Sf9 MVfGeY5kfGmxbjDhd5NigQ>? NI\kSHoyKGi{ NVTzc3RGUW6qaXLpeIlwdiCxZjDOJJRmem2rbnHsJIhmgGGqaYP0bYRqdmVvdHHn[4VlKGi3bXHuJGhFSUN6IHX4dJJme3OnZDDpckBU\jliY3XscJMh[W[2ZYKgNUBpeiCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCwMlk3KM7:TT6= MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd{M{ezN{c,OjF5MkO3N|M9N2F-
MCF7 MmHERY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MoPjOFghcHK| M4L5ZWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMT63JO69VS5? MlW2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2NkWwPVEoRjJ{NE[1NFkyRC:jPh?=
BE(2)-C M1W0cWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NWPBSGZyPzJiaILz NGTqOVNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFLFLFIqNUNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JGFt[W2jcjDicJVmKGG|c3H5MEBKSzVyIE2gNE4xPSEQvF2u MnvsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7M{KzNVYoRjJ{OUOyN|E3RC:jPh?=
Sf9 NELFenRHfW6ldHnvckBie3OjeR?= M37l[VIhcHK| MojiTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOkBmgHC{ZYPz[YQhcW5iU3[5JINmdGy|IHnuZ5Vj[XSnZDDmc5IhOiCqcoOgeZNqdmdiUljLT{1C[yCobIXvdo9o\W6rYzDzeYJ{fHKjdHWsJGlEPTBiPTCwMlAxOTJizszNMi=> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzByOUKwN{c,OjNyMEmyNFM9N2F-
Sf9 M2rhVWZ2dmO2aX;uJIF{e2G7 NVPabXRuOiCqcoO= NVTnN5JIUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOSCneIDy[ZN{\WRiaX6gV4Y6KGOnbHzzJIlv[3WkYYTl[EBnd3JiMjDodpMhfXOrbnegVmhMUy2DYzDmcJVwem:pZX7pZ{B{fWK|dILheIUtKEmFNUCgQUAxNjByNTFOwG0v MoXtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyMEmyNFMoRjJ|MEC5NlA{RC:jPh?=
A2780 NUD0RWp6S3m2b4TvfIlkcXS7IHHzd4F6 MXu3NkBpenN? NICxUZFEgXSxdH;4bYNqfHliYXfhbY5{fCClaYPwcIF1cW5icnXzbZN1[W62IHj1cYFvKEF{N{iwJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkKyJO69VS5? NXfIfld1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
A2780 MmC5SpVv[3Srb36gZZN{[Xl? MX[xPEBpenN? MlTsTY5pcWKrdHnvckBw\iCKRFHDJIlvKGOrc4DsZZRqdiC{ZYPpd5RidnRiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwMkWg{txONg>? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
A2780 NX3JTHZmS3m2b4TvfIlkcXS7IHHzd4F6 MUe3NkBpenN? NWe2Wo5CS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwMkmg{txONg>? M2D4OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
A2780 MXPGeY5kfGmxbjDhd5NigQ>? MW[xPEBpenN? NUnrOXJYUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEF{N{iwJINmdGy|IHHmeIVzKDF6IHjyd{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkSzJO69VS5? NITWU|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
Sf9 M3S5dGZ2dmO2aX;uJIF{e2G7 NFKzRoRKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWM3KGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{9{\jliY3XscJMhfXOrbnegVmhMU0GlIHHzJJN2[nO2cnH0[UwhUUN3MDC9JFAvODByOTFOwG0v M4fpPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
Sf9 NW\2dJZqTnWwY4Tpc44h[XO|YYm= NFLJWlBKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWMyKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{9{\jliY3XscJMhfXOrbnegVmhMU0GlIHHzJJN2[nO2cnH0[UwhUUN3MDC9JFAvODB3IN88UU4> MlLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
Sf9 MUPGeY5kfGmxbjDhd5NigQ>? NWLURZZJUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOiCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMwe2Z7IHPlcIx{KHW|aX7nJHJJU0uDYzDhd{B{fWK|dILheIUtKEmFNUCgQUAxNjByNTFOwG0v M2HrN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
Sf9 MUfGeY5kfGmxbjDhd5NigQ>? MnP3TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDNVAh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzM5NnQSClZXzsd{B2e2mwZzDSTGtMSWNiYYOgd5Vje3S{YYTlMEBKSzVyIE2gNE4xODlizszNMi=> MnHYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
Sf9 M17aTWZ2dmO2aX;uJIF{e2G7 M{LPTWlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR|EyKGW6cILld5Nm\CCrbjDiZYN2dG:4aYL1d{9{\jliY3XscJMhfXOrbnegVmhMU0GlIHHzJJN2[nO2cnH0[UwhUUN3MDC9JFAvODF5MzFOwG0v Moj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
Sf9 NWnBN3JSTnWwY4Tpc44h[XO|YYm= NX3ad5M{UW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEQCCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMwe2Z7IHPlcIx{KHW|aX7nJHJJU0GlS1HjJIF{KHO3YoP0doF1\SxiSVO1NEA:KDBwMUK5JO69VS5? NEnBXVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
MOLT4 MYnDfZRwfG:6aXPpeJkh[XO|YYm= NFzHUZE4OiCqcoO= MmPhR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUW9NXDRiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFQ1u4JIF{e2G7LDDJR|UxKD1iMD6wN{DPxE1w MnP5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkC2NlcoRjJ2OU[wOlI4RC:jPh?=
A549 M3m0dGN6fG:2b4jpZ4l1gSCjc4PhfS=> NF\te|M4OiCqcoO= MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhS0ONODDhd5NigSxiSVO1NEA:KDBwMEWg{txONg>? MlXCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkC2NlcoRjJ2OU[wOlI4RC:jPh?=
MCF7 NG[5UHZEgXSxdH;4bYNqfHliYYPzZZk> MVi3NkBpenN? M2XnRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFQ1u4JIF{e2G7LDDJR|UxKD1iMD6wOkDPxE1w M1[4VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wOlI4Lz5{NEm2NFYzPzxxYU6=
HL60 MUfDfZRwfG:6aXPpeJkh[XO|YYm= MUG3NkBpenN? M1fNVmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhNPjBiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDd{IHjyd{BjgSCFQ1u4JIF{e2G7LDDJR|UxKD1iMD6wPEDPxE1w MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4ME[yO{c,OjR7NkC2Nlc9N2F-
BGC823 NEDmeG1EgXSxdH;4bYNqfHliYYPzZZk> NF7DU5A4OiCqcoO= NEXzSmJEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBDT0N6MkOgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IEeyJIhzeyCkeTDDR2s5KGG|c3H5MEBKSzVyIE2gNE4xQCEQvF2u MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4ME[yO{c,OjR7NkC2Nlc9N2F-
HuH7 M{PzR2N6fG:2b4jpZ4l1gSCjc4PhfS=> MYS3NkBpenN? NVfWUnpFS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUHWKNzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiN{KgbJJ{KGK7IFPDT|gh[XO|YYmsJGlEPTBiPTCwMlEh|ryPLh?= NXS1d2xxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5OlA3OjdpPkK0PVYxPjJ5PD;hQi=>
U937 NH7ocIFEgXSxdH;4bYNqfHliYYPzZZk> M2f6[lczKGi{cx?= MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDVPVM4KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhS0ONODDhd5NigSxiSVO1NEA:KDBwMTFOwG0v M{flcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wOlI4Lz5{NEm2NFYzPzxxYU6=
HeLa MUTDfZRwfG:6aXPpeJkh[XO|YYm= NGXrRmU4OiCqcoO= NEHmOpZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB5MjDodpMh[nliQ1PLPEBie3OjeTygTWM2OCB;IECuNVEh|ryPLh?= M17idlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OU[wOlI4Lz5{NEm2NFYzPzxxYU6=
NCI-H1975 MnXLR5l1d3SxeHnjbZR6KGG|c3H5 NX7GdFVsPzJiaILz MXrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDOR2kuUDF7N{WgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IEeyJIhzeyCkeTDDR2s5KGG|c3H5MEBKSzVyIE2gNE4zOSEQvF2u NH3KWo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEm2NFYzPyd-MkS5OlA3Ojd:L3G+
K562 NGmwfIJEgXSxdH;4bYNqfHliYYPzZZk> MnTQO|IhcHK| MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDLOVYzKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA4OiCqcoOgZpkhS0ONODDhd5NigSxiSVO1NEA:KDBwNEGg{txONg>? MmC0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7NkC2NlcoRjJ2OU[wOlI4RC:jPh?=
HEK293 NEjlPY9EgXSxdH;4bYNqfHliYYPzZZk> MYq5OkBpenN? MUTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDISWszQTNiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOTZizszNMi=> NV24bIFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVYyODJpPkK1OVU3OTB{PD;hQi=>
LO2 NGnVeIpEgXSxdH;4bYNqfHliYYPzZZk> NVfQTlJwQTZiaILz NV;XRo43S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVE9{IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjJzIN88UU4> M2THXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUW2NVAzLz5{NUW1OlExOjxxYU6=
HeLa NFKyfYlEgXSxdH;4bYNqfHliYYPzZZk> M1X2OVk3KGi{cx?= MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJIFnfGW{IEm2JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTCwMlg2KM7:TT6= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3NkGwNkc,OjV3NU[xNFI9N2F-
HepG2 M3T1WGN6fG:2b4jpZ4l1gSCjc4PhfS=> Mn3pPVYhcHK| Ml3KR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvQTZizszNMi=> MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3NkGwNkc,OjV3NU[xNFI9N2F-
U2OS NIfY[5dEgXSxdH;4bYNqfHliYYPzZZk> NYLBUIQyQTZiaILz NETsXI5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWOk:VIHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAyPC57MzFOwG0v NFnDeYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1OlExOid-MkW1OVYyODJ:L3G+
Sf9 NFf4TmVHfW6ldHnvckBie3OjeR?= M4Xhd|kxKG2rboO= NEfpXWFKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJIh2dWGwIILlZ49u[mmwYX70JG4ufGW{bXnuZYwhT1OWLYTh[4dm\CCKRFHDOkApOSC2bzCxNlE2KHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gd4Y6KGOnbHzzJJV{cW6pIGLIT{1MMEGlKT3BUWMh[XNic4Xid5Rz[XSnIHnuZ5Vj[XSnZDDmc5IhQTBibXnud{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkCwNVcyPiEQvF2u MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB4MEe2OEc,OjdyNkC3OlQ9N2F-
Sf21 MmXxSpVv[3Srb36gZZN{[Xl? M2\5SlYxKG2rboO= MXHJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIHj1cYFvKHKnY3;tZolv[W62IFOteIVzdWmwYXygSmxCTy2KaYOteIFo\2WmIFjERWMyKChzIITvJFQ5OiC{ZYPp[JVmeyliZYjwdoV{e2WmIHnuJHNnOjFiY3XscJMhfXOrbnegVmhMNUtqQXOpMWFOSyCjczDzeYJ{fHKjdHWgbY5kfWKjdHXkJIZweiB4MDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFA3PTd2IN88UU4> NIHhV2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
insect cell M{X2[2Z2dmO2aX;uJIF{e2G7 M{TiW|YxKG2rboO= MofNTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDoeY1idiC{ZXPvcYJqdmGwdDDDMZRmem2rbnHsJGdUXC22YXfn[YQhUESDQ{KgLFEhfG9iNEi4JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIGLIT{1MMEGlKT3BUWMh[XNic4Xid5Rz[XSnIHnuZ5Vj[XSnZDDmc5IhPjBibXnud{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkCxNFUyKM7:TT6= NEm3dHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
insect cell NYT6ZVVHTnWwY4Tpc44h[XO|YYm= Mm\LOlAhdWmwcx?= NV;sTY9MUW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBpfW2jbjDy[YNwdWKrbnHueEBENXSncn3pcoFtKEircz30ZYdo\WRiSFTBR|MhMDFidH:gOFI5KHKnc3nkeYV{MS:qdX3hckBz\WOxbXLpcoFvfCCQLYTldo1qdmGuIFfTWE11[WepZXSgUmNQWjJiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyC3c3nu[{BTUEtvSzjBZ{kuSU2FIHHzJJN2[nO2cnH0[UBqdmO3YnH0[YQh\m:{IE[wJI1qdnNiYomg[ox2d3Knc3PlcoNmKGG|c3H5MEBKSzVyIE2gNE4xOTFyNzFOwG0v MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB4MEe2OEc,OjdyNkC3OlQ9N2F-
insect cell NVHB[YFVTnWwY4Tpc44h[XO|YYm= M2r4UmlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiTj30[ZJucW6jbDDIbZMufGGpZ3XkJGhFSUNzMTCoNUB1dyB|NEegdoV{cWS3ZYOpJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOvZoFkfWyxdnnyeZMh\XiycnXzd4lwdiC|eYP0[Y0hfXOrbnegVmhMNUtqQXOpMWFOSyCjczDzeYJ{fHKjdHWgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOC5yMUGzO{DPxE1w MnvyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyNkC3OlQoRjJ5ME[wO|Y1RC:jPh?=
insect cell NF\1cXJHfW6ldHnvckBie3OjeR?= M2r0RlYxKG2rboO= MkT5TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCFLYTldo1qdmGuIFjpd{11[WepZXSgTGRCSzhiKEGgeI8hOzd5IILld4llfWW|KTDlfJBz\XO|ZXSgbY4hcW6|ZXP0JINmdGy|IIXzbY5oKFKKSz3LLGFkMS2NKFHjLU1CVUNiYYOgd5Vje3S{YYTlJIlv[3WkYYTl[EBnd3JiNkCgcYlveyCkeTDmcJVwemW|Y3XuZ4Uh[XO|YYmsJGlEPTBiPTCwMlMxPzRizszNMi=> NUL3SItURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewOlA4PjRpPkK3NFYxPzZ2PD;hQi=>
SHSY5Y M{m2RmZ2dmO2aX;uJIF{e2G7 MXy4JIhzew>? M2PxTGlvcGmkaYTpc44hd2ZiSFTBR|YhcW5iaIXtZY4hW0iVWUXZJINmdGy|IIXzbY5oKEKDVFPQJIF{KHO3YoP0doF1\SCjZoTldkA5KGi{czDifUBWUFCOQz3FV2kuVVNxTWOgZY5idHm|aYOsJGlEPTBiPTCwMlAxPzhizszNMi=> NGrWcVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[1NFkzPSd-Mke2OVA6OjV:L3G+
SHSY5Y MkjnSpVv[3Srb36gZZN{[Xl? MWG4JIhzew>? NWPoW2Q3UW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKFOKU2m1XUBk\WyuczD1d4lv\yCPQVygZZMhe3Wkc4TyZZRmKGGodHXyJFghcHK|IHL5JHVJWEyFLVXTTU1OWy:PUzDhcoFtgXOrczygTWM2OCB;IECuNFA6OSEQvF2u NUDS[3VuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OVA6OjVpPkK3OlUxQTJ3PD;hQi=>
Sf9 MV7GeY5kfGmxbjDhd5NigQ>? MVS0JIhzew>? M4nGN2lvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiR2PUMZRi\2enZDDISGFEOSCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMhcW6oZXP0[YQhW2Z7IHnud4VkfCClZXzsd{B2e2mwZzDNU2NRSUNiYYOgd5Vje3S{YYTlJIFnfGW{IESgbJJ{KGK7IGXIVGxENUWVST3NV{9OWyCjbnHsfZNqeyxiSVO1NEA:KDBwMEG1O{DPxE1w NHzPbpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[1NFkzPSd-Mke2OVA6OjV:L3G+
insect cell MWTGeY5kfGmxbjDhd5NigQ>? NYHa[YJCPCCqcoO= M132bGlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR|Yh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJIlv\mWldHXkJIlve2WldDDj[YxteyC3c3nu[{BDSVSFUDDhd{B{fWK|dILheIUh[W[2ZYKgOEBpenNiYomgWWhRVENvRWPJMW1UN02VIHHuZYx6e2m|LDDJR|UxKD1iMD6wNVYh|ryPLh?= NXrCcndMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OVA6OjVpPkK3OlUxQTJ3PD;hQi=>
SHSY5Y NVziemNyTnWwY4Tpc44h[XO|YYm= M3rJZVghcHK| MoP6TY5pcWKrdHnvckBw\iCKRFHDNUBqdiCqdX3hckBUUFO\NWmgZ4VtdHNidYPpcochVU:FUFHDJIF{KHO3YoP0doF1\SCjZoTldkA5KGi{czDifUBWUFCOQz3FV2kuVVNxTWOgZY5idHm|aYOsJGlEPTBiPTCwMlAyQDhizszNMi=> NH3hRoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{[1NFkzPSd-Mke2OVA6OjV:L3G+
HEK293T MlPJSpVv[3Srb36gZZN{[Xl? MUCzNEBucW6| MXnJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEiGQVOxJIV5eHKnc4Pl[EBqdiCKRVuyPVNVKGOnbHzzJJV{cW6pIFHjMWtIVEeNKFHjLU1OS0FiYYOgd5Vje3S{YYTlJIFnfGW{IEOwJI1qdnNiaX6gdJJme2WwY3Wgc4YhTFSWIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvODF7IN88UU4> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTVzOU[wOEc,Ojl3MUm2NFQ9N2F-
HEK293T Mn\RSpVv[3Srb36gZZN{[Xl? M33MUFMxKG2rboO= MlnyTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDtc5V{\SCKRFHDOkBmgHC{ZYPz[YQhcW5iSFXLNlk{XCClZXzsd{Bkdy2neIDy[ZN{cW6pIH3veZNmKEiGQUKgeZNqdmdiQXOtT2dNT0tqQXOpMW1ESSCjczDzeYJ{fHKjdHWgZYZ1\XJiM{CgcYlveyCrbjDwdoV{\W6lZTDv[kBFXFRiYomg[ox2d3Knc3PlcoNmKGG|c3H5MEBKSzVyIE2gNE4xOjhizszNMi=> NHvC[o49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWxPVYxPCd-Mkm1NVk3ODR:L3G+
NFF M{XmSWN6fG:2b4jpZ4l1gSCjc4PhfS=> MXzDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDOSmYh[2WubIOsJGlEPTBiPTCwMlIh|ryPLh?= NH3Yc489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEK0OVQxOid-M{CyOFU1ODJ:L3G+
insect cell M4DDfWZ2dmO2aX;uJIF{e2G7 NXzJZ25lUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXC22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hUESDQ{[g[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR|UxKD1iMD6wNFg2KM7:TT6= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN4NUi5Nkc,OzB|NkW4PVI9N2F-
Sf21 NWfkb2RXTnWwY4Tpc44h[XO|YYm= M2HEWmlvcGmkaYTpc44hd2ZiQz30[ZJucW6jbDDGUGFINUircz30ZYdo\WRiZoXscEBt\W6pdHigdoVkd22kaX7hcpQhcHWvYX6gTGRCSzFiZYjwdoV{e2WmIHnuJHNnOjFiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCkeTDmcJVwemW|Y3XuZ4Uu[mG|ZXSgZZN{[XluIFnDOVAhRSByLkCwPVQh|ryPLh?= NVPibZBuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlU5QTJpPkOwN|Y2QDl{PD;hQi=>
insect cell MXnGeY5kfGmxbjDhd5NigQ>? M4LQfWlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iSFTBR|MwVkORUkKg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR|UxKD1iMD6wNVI4KM7:TT6= MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN4NUi5Nkc,OzB|NkW4PVI9N2F-
insect cell MYjGeY5kfGmxbjDhd5NigQ>? MmfHTY5pcWKrdHnvckBw\iCFLYTldo1qdmGuIFfTWE11[WepZXSgdoVkd22kaX7hcpQhcHWvYX6gTGRCSzJiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwZnXjeIVlKGmwc3XjeEBk\WyuczD1d4lv\yCUSFvLLGFkMUGPQzDhd{B{fWK|dILheIUh[nliZnz1c5Jme2OnbnPlMYJie2WmIHHzd4F6NCCLQ{WwJF0hOC5yMk[3JO69VS5? NX7Q[JR6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlU5QTJpPkOwN|Y2QDl{PD;hQi=>
insect cell MXrGeY5kfGmxbjDhd5NigQ>? NF\QNotKdmirYnn0bY9vKG:oIFOteIVzdWmwYXygTIl{NXSjZ3fl[EBz\WOxbXLpcoFvfCCqdX3hckBJTEGFODDlfJBz\XO|ZXSgbY4h[mGldXzveolzfXNiaX7m[YN1\WRiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCkeTDmcJVwemW|Y3XuZ4Uu[mG|ZXSgZZN{[XluIFnDOVAhRSByLk[wPUDPxE1w MlPzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB|NkW4PVIoRjNyM{[1PFkzRC:jPh?=
A20 NUXST3ZUTnWwY4Tpc44h[XO|YYm= MmLsRolv\GmwZzDh[oZqdmm2eTD0c5diemS|IFjERWMyKCiKaYP0c45mKGSnYXPleJlt[XOnIEGpJIlvKG2xdYPlJGEzOCClZXzsd{wh\XiycnXzd4VlKGG|IHLpcoRqdmdiY3;ud5RidnRiKIDLbUktKEurIE2gNE4xODFyN{Kg{txONg>? MlfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF7NkC0PFkoRjFzOU[wOFg6RC:jPh?=
Hela NHzpTmNHfW6ldHnvckBie3OjeR?= MVTJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVt[SClZXzsd{BvfWOuZXHyJIV5fHKjY4TzJIJ6KG[udX;ybY1mfHKrYzDhd5NigSxiS3mgQUAxNjVyNDFOwG0v MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTV{MEW4NEc,OTl3MkC1PFA9N2F-
Sf9 MV\GeY5kfGmxbjDhd5NigQ>? M{XXPVkxKG2rboO= NGDqVlZKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJJJm[2:vYnnuZY51KGi3bXHuJG4ufGW{bXnuZYwhT1OWLYTh[4dm\CCKRFHDOkBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOtbY5n\WO2ZXSgV4Y6KGmwc3XjeEBk\WyuczD1d4lv\yCUSFvLLGFkMUGPQzDhd{B{fWK|dILheIUh[W[2ZYKgPVAhdWmwczDifUBndHWxcnnt[ZRmeixiS3mgQUAxNjByMEW4JO69VS5? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6OUS0NUc,Ojl3OEm0OFE9N2F-
Sf21 Mkf0SpVv[3Srb36gZZN{[Xl? MV[2NEBucW6| M4nIS2lvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iQz30[ZJucW6jbDDGUGFIN0ircz30ZYdo\WRiSFTBR|Eh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzMYlv\mWldHXkJHNnOjFiaX7z[YN1KGOnbHzzJJV{cW6pIGLIT2spSWNrQV3DJIF{KHO3YoP0doF1\SCjZoTldkA3OCCvaX7zJIJ6KG[udX;ybY1mfGW{LDDLbUA:KDBwMECwOlQh|ryPLh?= NVHURmZWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
insect cell NXTtfGZZTnWwY4Tpc44h[XO|YYm= M1u2eFYxKG2rboO= NF;jV5RKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJJJm[2:vYnnuZY51KGi3bXHuJGMufGW{bXnuZYwhUGm|LYTh[4dm\CCKRFHDN{9PNXSncn3pcoFtKEeVVD30ZYdo\WRicnXjc41jcW6jboSgbJVu[W5iTlPPVlIhMDN7NTD0c{A1QDlicnXzbYR2\XNrIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2ey2rbn\lZ5Rm\CCrboPlZ5Qh[2WubIOgeZNqdmdiUljLT{hC[ymDTVOgZZMhe3Wkc4TyZZRmKGGodHXyJFYxKG2rboOgZpkh\my3b4LpcYV1\XJuIFvpJF0hOC5yMEC4OEDPxE1w M3nM[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
insect cell Mn35SpVv[3Srb36gZZN{[Xl? NVv4S|YxPjBibXnudy=> Mnf5TY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDy[YNwdWKrbnHueEBpfW2jbjDDMZRmem2rbnHsJGdUXC22YXfn[YQhUESDQ{Kg[ZhxemW|c3XkJIlvKGKjY4Xsc5ZqenW|LXnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC|dXLzeJJifGViYX\0[ZIhPjBibXnud{BjgSCobIXvdolu\XSncjygT4khRSByLkCwN|U2KM7:TT6= MlPlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
insect cell M{D5SmZ2dmO2aX;uJIF{e2G7 NHzaN2NKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFjERWMyOCCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMucW6oZXP0[YQhcW6|ZXP0JINmdGy|IIXzbY5oKG[udX;yc4dmdmmlIIDldJRq\GViUljLT2FkKGG|IIP1ZpN1emG2ZTDifUBndHWxcnnt[ZRmeixiS3mgQUAxNjByNEe2JO69VS5? M3rNU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
insect cell M2jTeGZ2dmO2aX;uJIF{e2G7 Ml\rOlAhdWmwcx?= MWHJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKENvdHXycYlv[WxiSHnzMZRi\2enZDDISGFEQCBqMTD0c{A{PzdicnXzbYR2\XNrIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2ey2rbn\lZ5Rm\CCrboPlZ5Qh[2WubIOgeZNqdmdiUljLLGFkMUtqQXOpRW1EKGG|IIP1ZpN1emG2ZTDh[pRmeiB4MDDtbY5{KGK7IH\seY9zcW2ndHXyMEBMcSB;IECuNVQ2KM7:TT6= Ml\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
insect cell MlnsSpVv[3Srb36gZZN{[Xl? NUf5O|JEUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDOMZRmem2rbnHsJGhqey22YXfn[YQhUESDQ{GxJEgyKHSxIEO0O{Bz\XOrZIXld{kh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzMYlv\mWldHXkJIlve2WldDDj[YxteyC3c3nu[{BTUEuNKFHjLWFOSyCjczDzeYJ{fHKjdHWgZpkh\my3b4LpcYV1\XJuIFvpJF0hOC52MEGg{txONg>? Mn\jQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
GM15850 NU\meWNvTnWwY4Tpc44h[XO|YYm= NXP0VHM3OC5zIIXN MonvNVIhcHK| MXnJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gS20yPTh3MDDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckB1d3SjbDDhZ4V1gWyjdHXkJIhqe3SxbnWgcIV3\WxiYYSgNE4yKHWPIHHmeIVzKDF{IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NUPIVmY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
A549 Mli0SpVv[3Srb36gZZN{[Xl? M{fiOFAvOSC3TR?= M3m3TWlvcGmkaYTpc44hd2ZiU1nSWFIhcW5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{Bi[mmuaYT5JJRwKGmwZIXj[UBpgXCncnHj[ZR6dGG2aX;uJI9nKHS3YoXsbY4h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nzJIF1KDBwMTD1US=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF2OUi2NEc,OTdzNEm4OlA9N2F-
ST14A NEnvdmFHfW6ldHnvckBie3OjeR?= NVj2RXdbPzVibl2= NXrJS5prUW6mdXP0bY9vKG:oIGLFV3QwVlKVRj3jc5VxdGWmIILldJJme3Orb36gc4YhdHWlaX\ldoF{\SC2YXfn[YQhemG2IGLFNU9PWlOHIFLEUmYheHKxbX;0[ZIh[WO2aY\peJkh\XiycnXzd4VlKGmwIGPUNVRCKGOnbHzzJIF1KDd3IH7NJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHz1Z4ln\XKjc3WgZYN1cX[rdIm= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV4NUm5N{c,OTd3NkW5PVM9N2F-
HEK293 NHP5RphHfW6ldHnvckBie3OjeR?= M2PSWFYxOCCwTR?= MWTJcoNz\WG|ZTDpckBIWlB5ODDwdo91\WmwIHzleoVtKGmwIFjFT|I6OyClZXzsd{BifCB4MECgcm0h[nliaX3teY5w[myxdB?= M3LzcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NU[2O|MzLz5zN{W2Olc{OjxxYU6=
HEK293 M2XDXmZ2dmO2aX;uJIF{e2G7 MUW2NFAhdk1? M3e0WWlv[3KnYYPlJIlvKHC{b4TlbY4h\Gm|dXzmbYRmKGm|b33ldoF{\SCuZY\lcEBqdiCKRVuyPVMh[2WubIOgZZQhPjByIH7NJIJ6KGmvbYXuc4Jtd3R? NFfNeZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W2Olc{Oid-MUe1OlY4OzJ:L3G+
HeLa NV\CcJlYTnWwY4Tpc44h[XO|YYm= NGHMSYoyKHWP M{HSOVI1KGi{cx?= Ml;4TY5lfWO2aX;uJI9nKHBzNjDlfJBz\XO|aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMTD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91fGmwZx?= M4e4UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkSzNVEyLz5zN{[0N|EyOTxxYU6=
HeLa MVfGeY5kfGmxbjDhd5NigQ>? NIfFOIsyKHWP MlLhNlQhcHK| MkHMTY5lfWO2aX;uJI9nKHB{NzDlfJBz\XO|aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMTD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91fGmwZx?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ2M{GxNUc,OTd4NEOxNVE9N2F-
HeLa NVnBO4ZKTnWwY4Tpc44h[XO|YYm= NVu2W3NUOSC3TR?= NFLkZowzPCCqcoO= NYTvdI5nUW6mdXP0bY9vKG:oIICyNUBmgHC{ZYPzbY9vKGmwIHj1cYFvKEinTHGgZ4VtdHNiYYSgNUB2VSCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2dHnu[y=> M1P0PVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkSzNVEyLz5zN{[0N|EyOTxxYU6=
HCT116 NEjjbYpHfW6ldHnvckBie3OjeR?= M4D5[mlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{GgZZN{\XO|ZXSgZZMhcGm|dH;u[UBpgXCncnHj[ZR6dGG2aX;uJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCkeTDX[ZN1\XKwIHLsc5Q> Mm\BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOThyNUSyN|koRjF6MEW0NlM6RC:jPh?=
HCT116 M2nnTGZ2dmO2aX;uJIF{e2G7 NYTjTI1vUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOkBie3Onc4Pl[EBieyC2dXL1cIlvKGi7cHXyZYNmfHmuYYTpc44hcW5iaIXtZY4hUEOWMUG2JINmdGy|IHL5JHdme3Sncn6gZoxwfA>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDB3NEKzPUc,OThyNUSyN|k9N2F-
HEK293 STF NVj0OGd2TnWwY4Tpc44h[XO|YYm= NHTaPVEzPCCqcoO= M3zJVGFkfGm4YYTpc44hd2ZibX;1d4UhX262M3Ggd4lodmGuaX7nJIV5eHKnc4Pl[EBqdiCKRVuyPVMhW1SIIHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJJRz[W6|Y4LpdJRqd26jbDDhZ5Rqfmm2eTDh[pRmeiB{NDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mNWKjc3XkJIxi[3SjdHWg[IVpgWS{b3flcoF{\SCjc4PhfS=> NFjjZok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUCyNlY4PSd-MUmwNlI3PzV:L3G+
NCI-H661 MoXpSpVv[3Srb36gZZN{[Xl? NVLsW3J{OzByIH7N Mln5OkBpenN? M2DXdmlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBPS0lvSE[2NUBk\WyuczDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiaHnzeI9v\SCKNDDhZ4V1gWyjdHnvckBifCB|MECgcm0h[W[2ZYKgOkBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ NEjSWIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO4OVYxOCd-MUmzPFU3ODB:L3G+
NCI-H661 NYCwSFVDTnWwY4Tpc44h[XO|YYm= MlLNN|AxKG6P MoLLOkBpenN? NVTV[XBwUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKE6FST3IOlYyKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDhcJBp[S22dXL1cIlvKGGlZYT5cIF1cW:wIHH0JFMxOCCwTTDh[pRmeiB4IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NF3hVHA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO4OVYxOCd-MUmzPFU3ODB:L3G+
HL60 NXzFWHByTnWwY4Tpc44h[XO|YYm= M1PYclIxKHWpL33s MUOyOEBpenN? NF;mNJVKdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUEx4MDDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBpcXO2b37lJGg{KGGlZYT5cIF1cW:wIHH0JFIxKHWpL33sJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> M4\2clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUO0OFcyLz5zOUWzOFQ4OTxxYU6=
primary bronchial epithelial cell MlLJSpVv[3Srb36gZZN{[Xl? Mn\KNE4yKHWP M37iUGlvcGmkaYTpc44hd2ZiSFTBR|chcW5iaIXtZY4heHKrbXHyfUBjem:wY3jpZYwh\XCrdHjlcIlidCClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gcZV1[W62IF\k[Yx1[TVyODDDSnRTKHC{b4TlbY4h[XCrY3HsJJN2em[jY3WgcI9k[WyrenH0bY9vKGG2IECuNUB2VQ>? MnTWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O|g6RC:jPh?=
MCF7 NYO4bmRSTnWwY4Tpc44h[XO|YYm= MW[wMlEhfU1? MlPVNVYhcHK| NX\hfVlHUW6qaXLpeIlwdiCxZjDTTXJVOiCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJJR2[nWuaX6gbJlx\XKjY3X0fYxifGmxbjDheEAxNjFidV2gZYZ1\XJiMU[gbJJ{KGK7IGfld5Rmem5iYnzveC=> M4PkPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMEOwN|Q{Lz5{MECzNFM1OzxxYU6=
HeLa Mmn0SpVv[3Srb36gZZN{[Xl? MUOxJJVO NVS5eZFoOTBibXnudy=> MnPTTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhmVGFiY3XscJMh[XRiMTD1UUBi\nSncjCxNEBucW6|IHL5JIZtfW:{b33leJJq[yCjc4PhfS=> M4jWeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEW1PFQ5Lz5{MUC1OVg1QDxxYU6=
MCF7 NHrQbGhHfW6ldHnvckBie3OjeR?= M{[wOVAvPSC3TR?= NYTRdZN5OjRiaILz MXHJcohq[mm2aX;uJI9nKEOuYYPzJFEhcGm|dH;u[UBl\WGlZYT5cIF{\SCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJJVxNXKnZ4XsZZRqd25ib3[gdFIyKHC{b4TlbY4hdGW4ZXygZZQhOC53IIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6MEO2N{c,OjJ{OECzOlM9N2F-
MCF7 NW[5XWs6TnWwY4Tpc44h[XO|YYm= MmPDNE42KHWP NGO3fIwzPCCqcoO= MVnJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNiY3jhcodmKGmwIIT1ZpVtcW5iYXPleJlt[XSrb36gcIV3\WxiYYSgNE42KHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6MEO2N{c,OjJ{OECzOlM9N2F-
MCF7 MkO0SpVv[3Srb36gZZN{[Xl? MX:wMlUhfU1? NEm5WoIzPCCqcoO= M1TOe2lvcGmkaYTpc44hd2ZiSFTBR|EhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczD1dE1z\We3bHH0bY9vKG:oIHHj[ZR6dGG2ZXSgbIl{fG:wZTDIN{Bxem:2ZXnuJIxmfmWuIHH0JFAvPSC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz M2naeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkiwN|Y{Lz5{MkK4NFM3OzxxYU6=
PC12 MXvGeY5kfGmxbjDhd5NigQ>? NHjmcFAyOCC3TR?= NUnMUZJWPDhiaILz NYGyeo5HUW6qaXLpeIlwdiCxZjDISGFEPiCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDhZ4V1gWyjdHnvckBw\iCqaYP0c45mKEh|IHH0JFExKHWPIHHmeIVzKDR6IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKGGwYXz5d4l{ NXO3TXVJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
PC12 MVvGeY5kfGmxbjDhd5NigQ>? NILVZ2oyOCC3TR?= M3\kTlQ5KGi{cx?= M4HhS2lvcGmkaYTpc44hd2ZiSFTBR|YhcW5icnH0JHBEOTJiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFteGijLYT1ZpVtcW5iYXPleJlt[XSrb36gZZQhOTBidV2gZYZ1\XJiNEigbJJ{KGK7IGfld5Rmem5iYnzveJRqdmdiYX7hcJl{cXN? M4Lo[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
PC12 M{nHOWZ2dmO2aX;uJIF{e2G7 NF7DNVgyOCC3TR?= NIXreZg2KGSjeYO= M1vPWGlv\HWldHnvckBw\iCwZYXybZRmKG:3dHfyc5d1cCCrbjDyZZQhWENzMjDj[YxteyCjdDCxNEB2VSCjZoTldkA2KGSjeYO= NFvPZXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
SH-SY5Y NXv1dHlwTnWwY4Tpc44h[XO|YYm= NEH3V5UyOCC3TR?= NVmxUmpCPSCmYYnz MmTCTY5lfWO2aX;uJI9nKG6ndYLpeIUhd3W2Z4Lve5RpKGmwIHj1cYFvKFOKLWPZOXkh[2WubIOgZZQhOTBidV2gZYZ1\XJiNTDkZZl{ MoL5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
A549 M{K1[WFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NGnEOW8xNjRidV2= MU[yOEBpenN? MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgd4hzfW6tZX6gZY5lKHOqYYLwJJNp[XCnIHH0JFAvPCC3TTDh[pRmeiB{NDDodpMh[nlicHjhd4Uh[2:wdILhd5QhdWmlcn;zZ49xgQ>? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6NEGxNkc,OjV6OESxNVI9N2F-
HeLa NYTabHI1TnWwY4Tpc44h[XO|YYm= NFz1TI9KdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgeJVjfWyrbjDhZ4V1gWyjdHnvckBqdmO3YnH0[YQh\m:{IH;2[ZJvcWeqdDDifUBqdW23bn;mcJVwemW|Y3XuZ4UhdWmlcn;zZ49xcWNiYX7hcJl{cXN? M4fkXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NkGxPVE6Lz5{Nk[xNVkyQTxxYU6=
C127-LT NEfKd|hHfW6ldHnvckBie3OjeR?= NIOzU5UzODBibl2= NXnnbHNVOjRiaILz M1vYWmlvcGmkaYTpc44hd2ZiSFTBR{BqdiCvb4Xz[UBEOTJ5LVzUJINmdGy|IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiRVfGVEBmgHC{ZYPzbY9vKGG2IEKwNEBvVSCjZoTldkAzPCCqcoOgZpkh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcIm= NVTnemR4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVY{PzJpPkK2PVk3Ozd{PD;hQi=>
HeLa nuclear NHHF[mFHfW6ldHnvckBie3OjeR?= NIHSbW4yOCC2bzCyOUB2VQ>? MUSzNEBucW6| MV\Dc41x\XSrdHn2[UBqdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYTDueYNt\WG{IHPlcIx{KGG2IEGwJJRwKDJ3IIXNJJV{cW6pIFLvZ{1i[2W2eXytcJl{cW6nLVHNR{BieyC|dXLzeJJifGViYX\0[ZIhOzBibXnud{BjgSCOaX7le4VifmW{LVL1dosheGyxdDDhcoFtgXOrcx?= NIPQSoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OlM4Oid-Mk[5PVY{PzJ:L3G+
HeLa MYPGeY5kfGmxbjDhd5NigQ>? MU[yJJVO MkS3NlQhcHK| M4KxU2lvcGmkaYTpc44hd2ZiSFTBR|EhcW5iaIXtZY4hUGWOYTDj[YxteyCjc4Pld5Nm\CCjczDofZBmemGlZYT5cIF1cW:wIH;mJIhqe3SxbnWgTFMh[XRiMjD1UUBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NGDRVFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
HN13 NXnP[4lMTnWwY4Tpc44h[XO|YYm= M1XOZVI1KGi{cx?= MVHJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTG4yOyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gbIl{fG:wZTDIN{BNgXN7IHHj[ZR6dGG2aX;uJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHntcZVvd2[udX;y[ZNk\W6lZTDhd5NigQ>? NHz3NYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUCzNVA3Pid-MkmwN|ExPjZ:L3G+
HeLaS3 M4nKRmZ2dmO2aX;uJIF{e2G7 NIPZRWoyOCC3TR?= MVzJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVN[VN|IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFNMQSCjY3X0fYxifGmxbjDheEAyOCC3TTDt[YF{fXKnZDDpcY1m\GmjdHXsfUBjgSCrbX31co9jdG:2IHHuZYx6e2m| MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N|U9N2F-
HeLaS3 M1HGfGZ2dmO2aX;uJIF{e2G7 NFi2WYMyOCC3TR?= NX7USW9vOTZiaILz NY\Fd5FKUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHHTN{Bk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCqaYP0c45mKEh|S{mgZYNmfHmuYYTpc44h[XRiMUCgeW0hfHKnYYTl[EBnd3JiMU[gbJJ{KG[xbHzve4VlKGK7IHPvcZBwfW6mIIfhd4hwfXRiYX7kJI1m[XO3cnXkJJVxKHSxIEK0JIhzeyCkeTDpcY12dm:kbH;0JIFv[Wy7c3nz MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N|U9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
EGFR / STAT3 ; 

PubMed: 31289542     


Expression of EGFR and STAT3 in PC3 cells treated with TSA. PC3 cells were plated in 6-cm dishes, and 0.25, 0.5 or 1 µM TSA was added for 24 h. DMSO was added into the control group wells. Cells were collected and lysed with lysis buffer and the protein expression analyzed via western blotting.

Cyclin D1 / CDK6 / CDK4 / p-RB / RB; 

PubMed: 31289542     


Expression of cyclin D1, CDK6, CDK4 and RB, and the phosphorylation of RB, was detected in PC3 cells treated with TSA for 24 h. 

MMP-1 / MMP-3 / MMP-13 / TIMP-1; 

PubMed: 27431944     


Chondrocytes were pretreated with increasing concentrations of TSA for 2 h, followed by stimulation with 5 ng/ml IL-1β for 24 h.

Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1); 

PubMed: 27431944     


Chondrocytes were treated with increasing concentrations of TSA for 24 h.

α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2; 

PubMed: 23802098     


Histone acetylation occurs directly at the promoter region of IL-23R. The ChIP assay demonstrates that TSA treatment results in an increase in the acetylation of histone H3 and H4. A549 cells were cultured in the presence or absence of TSA (250 ng/mL) for a period of 24 h. Subsequently, a ChIP assay was performed using the following antibodies; pan acetyl-histone H3 (H3Ac), pan acetyl-histone H4 (H4Ac), acetyl-histone H3 Lys 9 (H3K9Ac), acetyl-histone H3 Lys 9/14 (H3K9/14ac), acetyl-histone H3 Lys 9 phosphoSer10 (H3K9S10), methyl-histone H3 Lys 4 (H3K4Me), di methyl-histone H3 Lys 4 (H3K4Me2), and di methyl-histone H3 Lys 9 (H3K9Me2). Input DNA serves as a positive control as recommended by the manufacturer (Diagenode). A no antibody control was included to test for non-specific binding (UT, untreated; TSA, Trichostatin A).

31289542 27431944 23802098
Immunofluorescence
α-C/EBPβ / α-HP1α ; 

PubMed: 21122806     


3T3-L1 preadipocytes were grown on coverslips for three days and induced to differentiate by treatment with MDI (48 h) in the presence of vehicle (-TSA) or 87 nM Trichostatin A (+TSA). Under these experimental conditions: A-H Cells were fixed and permeabilized in cold methanol, subjected to IIF with the indicated antibodies, and nuclei were counterstained with DAPI. Scale bar, 5 μm. 

α-HDAC1 ; 

PubMed: 29045501     


MCF-7 cells were treated with indicated concentrations of trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels.

α-tubulin / acetylated histone 3; 

PubMed: 27957719     


Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775.

21122806 29045501 27957719
Growth inhibition assay
Cell viability (A549 cells); 

PubMed: 27571418     


Cytotoxicity of histamine determined by MTT assay in A549 cells.

Cell viability (MDA-MB-231 cells); 

PubMed: 27548148     


The human breast cancer cells (MDA-MB-231) were incubated with various concentrations of TSA (0–300 nM) for 24 h. Cell viability was measured by WST-8.

Cell viability (PC3 cells); 

PubMed: 31289542     


PC3 cells were plated in a 96-well plate and treated with different doses of TSA (0, 0.25, 0.5 and 1 µM) for 72 h; an MTT assay was used to measure the effects of TSA on PC3 cell proliferation.

27571418 27548148 31289542
体内研究 Administration of Trichostatin A 按 0.5 mg/kg 剂量处理N-甲基-N-亚硝基脲致癌物诱发的大鼠乳腺癌模型,持续处理4周,具有有效的抗癌活性,即使按按高达5 mg/kg剂量处理也没有任何可测量到的毒性。[1]Trichostatin A 按10 mg/kg 剂量单独腹腔注射给药非转基因和脊髓性肌萎缩症(SMA)模型鼠,导致乙酰化的 H3 和 H4组蛋白水平提高,也导致活运动神经元(SMN) 基因表达稍微提高。Trichostatin A 每天按10 mg/kg剂量处理SMA模型鼠,促进存活, 减轻体重下降,且增强运动行为。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

体外HDAC活性 :

从每种乳腺癌细胞系(MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51,或 SK-BR-3)中准备全部细胞提取物。在溶于0.1% (v/v)乙醇的不同浓度Trichostatin A存在时,或者使用0.1% (v/v)乙醇作为对照, 20 μL 细胞粗提取物(~2.5 ×105 个细胞)与 1 μL ~1.5 × 106 cpm [3H]乙酰标记的组蛋白 H4 肽底物(第2-20位NH2-末端残基) 在25oC下温育60分钟。使用50 μL 1 M HCl/0.16 M 乙酸对每组 200-μL 反应进行淬火,然后使用600 μL 乙酸乙酯进行抽提,通过闪烁计数器测量释放的[3H]乙酸。使用非线性回归曲线拟合抑制数据,绘制合适的剂量反应曲线,而测定IC50。
细胞实验:[1]
- 合并
  • Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3
  • Concentrations: 溶于无水酒精,终浓度为~10 μM
  • Incubation Time: 96小时
  • Method: 使用不同浓度Trichostatin A处理细胞96小时。处理后,使用sulforhodamine B 比色分析测定细胞增殖。通过台酚蓝染色排除法测定细胞活力。
    (Only for Reference)
动物实验:[1]
- 合并
  • Animal Models: 携带NMU诱导的肿瘤的近交系(Ludwig/Wistar/Olac)处女鼠
  • Dosages: ~5 mg/kg/day
  • Administration: 皮下注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 23 mg/mL (76.05 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
1.5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 302.4
化学式

C17H22N2O3

CAS号 58880-19-6
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04606771 Recruiting Drug: Osimertinib + Savolitinib|Drug: Savolitinib + Placebo Non-Small Cell Lung Cancer AstraZeneca September 28 2020 Phase 2
NCT03901053 Recruiting Device: Reaktiv AFO|Device: PhatBrace AFO Foot Injuries and Disorders Jason Wilken|Minneapolis Veterans Affairs Medical Center|Walter Reed National Military Medical Center|Henry M. Jackson Foundation for the Advancement of Military Medicine|Center for Veterans Research and Education|University of Delaware|Johns Hopkins Bloomberg School of Public Health|University of Iowa February 27 2020 Not Applicable
NCT03887650 Recruiting Drug: Liposomal Bupivicaine 1.3%|Drug: Bupivacaine 0.5% Post-operative Pain|Total Shoulder Arthroplasty|Osteoarthritis of the Shoulder|Pain Management Hartford Hospital January 14 2019 Phase 4

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

  • 回答:

    Our S1045 Trichostatin A (TSA) is R enantiomer.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

相关HDAC产品

Tags: 购买Trichostatin A (TSA) | Trichostatin A (TSA)供应商 | 采购Trichostatin A (TSA) | Trichostatin A (TSA)价格 | Trichostatin A (TSA)生产 | 订购Trichostatin A (TSA) | Trichostatin A (TSA)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID