Trichostatin A (TSA)

For research use only. Not for use in humans.

目录号：S1045 中文名称：曲古柳菌素A

CAS No. 58880-19-6

Trichostatin A (TSA)是一种HDAC抑制剂，无细胞试验中IC50为1.8 nM左右。

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2mg	RMB 2022.93	现货
5mg	RMB 5053.26	现货
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客户使用Selleck生产的Trichostatin A (TSA)发表文献179篇：

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产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述

Trichostatin A (TSA)是一种HDAC抑制剂，无细胞试验中IC50为1.8 nM左右。

靶点

HDAC ^[1]
(Cell-free assay)

~1.8 nM

体外研究

Trichostatin A 抑制八种乳腺癌细胞系，包括MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3增殖，平均IC50为124.4 nM (范围为26.4-308.1 nM),作用于表达ERα的细胞系比作用于表达 ERα阴性的细胞系更有效。Trichostatin A作用于全部乳腺癌细胞系，抑制HDAC活性，平均IC50为 2.4 nM (范围为0.6-2.6 nM), 产生显著的组蛋白 H4高度乙酰化。^[1]与 Trapoxin (TPX) 和 Chlamydocin 有效抑制HDAC1 或 HDAC4而不是 HDAC6不同，Trichostatin A 抑制这些HDACs，且抑制程度相似， IC50 分别为6 nM, 38 nM, 和8.6 nM。^[2] Trichostatin A (100 ng/mL) 作用于MIA PaCa-2细胞，通过招募p300和 PCAF进入Sp1-NF-Y HDAC复合体，而诱导转化生长因子βⅡ型受体(TβRII) 表达，复合体与TβRII 启动子的DNA片段结合，伴随着Sp1乙酰化，且与复合体相关的HDAC数理全部降低。^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
HeLa	NV\G[2VVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MorhNVAwOzBxNUCgcm0>	M3;KflczKGh?	M1ftbGROW09?	MVTpcoNz\WG\|ZYOgeIhmKG63bXLldkBw\iCPTWCgLO6V\|qivKTDsc5N{KGOnbHzzJIRwe2ViZHXw[Y5l\W62bIm=	Mnf5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNkW3OFgoRjJ\|MU[1O\|Q5RC:jPh?=
HeLa	NX7sXmhVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NYrzNWFlOjBibl2=	NXPOfFRJPzJiaB?=	NX6zWZR2TE2VTx?=	MU\JR\|UxKG:oIEKwcm0>	NUXidYhwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa	NFnwVVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NXHBfIgyPDBibl2=	M2jQRVQ5KGh?	MXzEUXNQ	NET2VYhKSzVyIH;mJFQxdk1?	NYDtNm9ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa	NYfUUZFUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MXGxNFAhdk1?	NXfa[JJ5OjRiaB?=	MmXwSG1UVw>?	M3OxdmlEPTBib3[gNVAxdk1?	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF4NUe0PEc,OjNzNkW3OFg9N2F-
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HLEB3	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MoXPNE4zKM7:TR?=	M3XWfVQ5KGh?	Mnf1SG1UVw>?	NX:1UHk5e3WycILld5NmeyClZXzsJJBzd2yrZnXyZZRqd25idHjyc5VocCC\|dYDwdoV{e2mwZzD0bIUhWEl\|Sz;Bb5QtKHB\|OF3BVGsh[W6mIFXST\|EwOiC\|aXfuZYxqdmdicHH0bJdigXN?	NUewcVU5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVc5PzhpPkK0NVU4QDd6PD;hQi=>
SRA01/04	NV\nZllWT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NY\1RVVZOC5{IN88US=>	NYL3c2tEPDhiaB?=	M3[2[mROW09?	MlfZd5VxeHKnc4Pld{Bk\WyuIIDyc4xq\mW{YYTpc44hfGi{b4XnbEB{fXCycnXzd4lv\yC2aHWgVGk{Uy:Da4SsJJA{QE2DUFugZY5lKEWUS{GvNkB{cWewYXzpcocheGG2aIfhfZM>	NF:xT4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1O\|g4QCd-MkSxOVc5Pzh:L3G+
A2780	M4PBPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MoOzNE4xPS1{IN88US=>	NHG4cVMyOC9{ND:0PEBp	NGjIT2tFVVOR	M2LPeI1m\GmjdHXzJIdzd3e2aDDhdpJme3RiaX6gZUBkd26lZX70doF1cW:wLTDhcoQhfGmvZT3k[ZBmdmSnboSgcYFvdmW{	NIPEVG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKyN\|gxOSd-MkSyNlM5ODF:L3G+
SKOV3	M2XZ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MlzqNE4xPS1{IN88US=>	NXHzU4JwOTBxMkSvOFghcA>?	MkfjSG1UVw>?	NX[0c\|FOdWWmaXH0[ZMh\3Kxd4ToJIFzemW\|dDDpckBiKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK=	MlHxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MkO4NFEoRjJ2MkKzPFAyRC:jPh?=
Huh7	NXLidZZ3TnWwY4Tpc44hSXO\|YYm=	MViwMlEwOC53L{GuNEDPxE1?	NXixeWdpOjSq		NFy2PGp{fXCycnXzd4V{KFmWSFTDNkBo\W6nIHX4dJJme3Orb36=	MoPXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{Nkm2O\|IoRjJ2Mk[5OlczRC:jPh?=
hMSCs	MYPGeY5kfGmxbjDBd5NigQ>?	M3nUPFYvOjVibl2=	MlnLNlQhcA>?	M1rzUWROW09?	NGDlb2t{fGGkaXzpfoV{KEirc4TvcoUhSWOndInsZZRqd25iYX7kJJRp\SCHeIDy[ZN{cW:wIH;mJHBtfXKrcH;0[Y51KEenbnXz	M{HT[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2M{GyN\|U3Lz5{NEOxNlM2PjxxYU6=
HT29	MkjMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NI\Kd3AyQDBibl2=	NEPP[oU4OiCq		NWX4O5lXUUN3MDDv[kAyQDBibl2=	NXKxUXFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlgzPjVpPkK0N\|Y5OjZ3PD;hQi=>
RPE	M{nGemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NU\wbWU3OC5{L{CuOE8xNjhxMTFOwG0>	NH7EWpkzPC92OD:3NkBp	NILmd5lFVVOR	NVn5V4t5cW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44h[nliY3XscEBkgWOuZTDhdpJme3R?	NXi0XJA1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OVY3ODJpPkK0OFU3PjB{PD;hQi=>
U87 GBM	NEm2dGlHfW6ldHnvckBCe3OjeR?=	Mn:zNVAxNzVyMDDOcS=>	NEfNZ2I1QCCq	NFjwVZIyODBnIHX0bIFvd2x?	Ml3qTY5lfWOnczDT[Y5me2OnbnPlMWxqc2ViQXz0[ZJifGmxboOgbY4hVnWlbHXhdkBOd3KyaH;sc4d6	NFXxOIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NES2OFg1OSd-MkS0OlQ5PDF:L3G+
U87 GBM	NIe2OpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MX6xNFAwOzByL{WwNEBvVQ>?	NWK2VYVNPzJiaB?=	Ml;PNVAxLSCndHjhco9t	MX\y[YR2[2W\|IH3lZY4h[2WubDDueY1j\XJiYomgN\|EtKDV2LDDhcoQhPTkEoDW=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDR4NEi0NUc,OjR2NkS4OFE9N2F-
ACP03	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1TCVVI2OC9\|NUCvOVAxdk1?	NH;0RXozPGh?		MoT2[IVkemWjc3XzJINmdGxidnnhZoltcXS7IH;mJIFxeHKxeHntZZRmdHliN{FCpEUh[XRiMkWwxsBvVQ>?	NX71c4x6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2Olg2PDdpPkK0OlY5PTR5PD;hQi=>
ACP02	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NV[4cG0zOjVyL{O1NE82ODCwTR?=	NWL0RYNxOjSq		NEnPclFl\WO{ZXHz[ZMh[2WubDD2bYFjcWyrdImgc4Yh[XCycn;4bY1ifGWueTC3NOKhLSCjdDCyOVDDqG6P	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ4OEW0O{c,OjR4Nki1OFc9N2F-
HeLa	NXHCR5NvTnWwY4Tpc44hSXO\|YYm=	NYjJSI43OW2P	NWPHdlM5OC53aB?=		MkLDZYJwdGm\|aHXzJJRp\SC2b4ThcEBJTEGFIHHjeIl3cXS7	NGHWZZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEewO\|Q4PCd-MkS3NFc1PzR:L3G+
HMEC-1	NF36TWlHfW6ldHnvckBCe3OjeR?=	NEHXWnI{ODCwTR?=	Mk\hNlRp		NWntRnQ{cW6lcnXhd4V{KGW6cILld5Nqd25ib3dCpHZGT0[UM9MgcXJPSQ>?	NWfBU2pFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3NVA3OzFpPkK0O\|ExPjNzPD;hQi=>
HeLa	NHTRN3RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NWPsZ5lEOs7:TR?=	MVm0PIg>		NVLWcZZF\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIFjd3W2IEK1KS=>	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh2NkGzOUc,OjR6NE[xN\|U9N2F-
LNCaP	M2L3Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3nNRVExOC1zMECwcm0>	Mlv3NlRp		M{DMPWlEPTBib3[gN\|Axdk1?	MmHDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6NUS2OVgoRjJ2OEW0OlU5RC:jPh?=
PC3	MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NVTiUGV3OTByLUGwNFBvVQ>?	MXyyOIg>		M3rhTmlEPTBib3[gN\|Axdk1?	M{CzNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OEW0OlU5Lz5{NEi1OFY2QDxxYU6=
CNE2	NEHtNnNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1TYVVExOC14MEDuUS=>	NHjuSGgzPC92OD:3Nog>		NUjxT4RncW6qaXLpeJMhfGinIIDyc4xq\mW{YYTpc44hcW5iYTD0bY1mNSCjbnSg[I9{\S2mZYDlcoRmdnRibXHucoVz	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl4OUmwNUc,OjR7Nkm5NFE9N2F-
HeLa	MWXGeY5kfGmxbjDBd5NigQ>?	MkPtNlUxdk1?	MkTXNVZp	M{HkNmROW09?	MULpcoNz\WG\|ZYOgR3lROUFzIH3SUmEh\XiycnXzd4lwdsLi	NWLvdmFnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxNVY3QDhpPkK1NVE3Pjh6PD;hQi=>
A549	MmDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NFywUogxNjBzLUCuPe69VQ>?	NXj2NohqPzKq	M2mxVmROW09?	M3W1RWlEPTBib3[gNE4xPs7:TR?=	M1faflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
SKOV-3	NYLoUIlGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHTjTXcxNjBzLUCuPe69VQ>?	NGniR2Y4Omh?	MVnEUXNQ	NVvQVZk4UUN3MDDv[kAxNjN{zszN	NX7YfZFURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOlk1QTFpPkK1NVY6PDlzPD;hQi=>
A549	M2LMbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NHPSeXQxNjFvMd88US=>	Mo\qOFhp	NE\Id\|hFVVOR	M2L4TGlEPTBib3[gNE4zQM7:TR?=	NIPoUpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG2PVQ6OSd-MkWxOlk1QTF:L3G+
SKOV-3	M{nzbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mo\jNE4yNTIQvF2=	NVjJZ2h7PDiq	MWrEUXNQ	Mn7iTWM2OCCxZjCwMlfPxE1?	M3izT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3MU[5OFkyLz5{NUG2PVQ6OTxxYU6=
A549	NV3FWVNwT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NInOSHIyNTFyzszN	MkOyNlRp	NXLZZlQ5TE2VTx?=	NETpb\|ZKSzVyIH;mJFMvOs7:TR?=	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
SKOV-3	NH;6dYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NH\yeIgyNTFyzszN	M4jqU\|I1cA>?	MV7EUXNQ	MWLJR\|UxKG:oIEWuOu69VQ>?	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF4OUS5NUc,OjVzNkm0PVE9N2F-
MCF7	Mmm4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3TYcVAuPjBybl2=	M3nydlI1cA>?	M2C5WGROW09?	NGTyOVJKSzVyIH;mJFc2dk1?	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTF7MkeyNUc,OjVzOUK3NlE9N2F-
MDA-MB-231	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MlrkNE03ODCwTR?=	NWOwe\|lsOjSq	NFPzOndFVVOR	NUntWnViUUN3MDDv[kAyODCwTR?=	NF74fms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG5NlczOSd-MkWxPVI4OjF:L3G+
A431	MUDGeY5kfGmxbjDBd5NigQ>?	MnzNOVBvVQ>?	NWLCd5dqOi94L{GyM\|I1cA>?	NG\mcGpFVVOR	MXLhZ5RqfmG2ZYOgdFIyKGGwZDDpcohq[mm2czDBWGY{KGW6cILld5Nqd25?	NUHuNIh4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzO\|ExPjlpPkK1N\|cyODZ7PD;hQi=>
A431	NFnxemdCeG:ydH;zbZMhSXO\|YYm=	NUn2RpQ6Oi9zMD:1NE8yODCwTR?=	M1PEUFQ5cA>?	NV3NWGdDTE2VTx?=	NIrTcopqdmirYnn0d{B1cGViY3XscEBoem:5dHi=	M1rmSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3M{exNFY6Lz5{NUO3NVA3QTxxYU6=
PC3	NELjeWVHfW6ldHnvckBCe3OjeR?=	NFfjR3cxNjIQvF2=	MnvkOFhp	NH3OXm1FVVOR	M33Xbolv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ?	NV;mUplWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N\|Q6QTdpPkK1OFM1QTl5PD;hQi=>
SW480	MnzaSpVv[3Srb36gRZN{[Xl?	NF32VIMxNjIQvF2=	NEfOeG41QGh?	NVHZcVN5TE2VTx?=	M3:5eolv\HWlZYOgbY5kemWjc3Wgc4YhUESDQ{GgZY5lKEiGQVOyJI9vKFOudXeg[4Vv\XNicILvcY91\XJ?	NHrSSHg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUSzOFk6Pyd-MkW0N\|Q6QTd:L3G+
PC3	NXq1bVhHTnWwY4Tpc44hSXO\|YYm=	MVewMlHPxE1?	M16wcVQ5cA>?	NWPFSXR[TE2VTx?=	NGXG[ndifHSnboXheIV{KGmwdnHzbY9vKGGwZDDtbYdz[XSrb36=	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR\|NEm5O{c,OjV2M{S5PVc9N2F-
PC3	NIrHfZVHfW6ldHnvckBCe3OjeR?=	M2DOWlAvOc7:TR?=	M3qyXFQ5cA>?	NX\hZXlsTE2VTx?=	NHjXcYlz\X[ncoPld{BGVVR?	M2XieFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
SW480	M1jvU2Z2dmO2aX;uJGF{e2G7	MoLXNE4y\|ryP	Mn[2OFhp	NHvtb3VFVVOR	MX\heJRmdnWjdHXzJIlvfmG\|aX;uJIFv\CCvaXfyZZRqd25?	M4\NW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NEO0PVk4Lz5{NUSzOFk6PzxxYU6=
SW480	NEHLTFFHfW6ldHnvckBCe3OjeR?=	NVfoTW55OC5zzszN	MmHzOFhp	NUOwT3R6TE2VTx?=	MVjy[ZZmenOnczDFUXQ>	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR\|NEm5O{c,OjV2M{S5PVc9N2F-
MEFs	MVPGeY5kfGmxbjDBd5NigQ>?	NHXM[Ho2\|ryP	Mn\3NVZp		M1fGVolv[3KnYYPld{B1cGViRWDFR{BifHSjY3jt[Y51NCCWaYKg[IVtcX[ncomgZY5lKHSqZTDl[oZq[2mnbnP5JI9nKHCnZHXzeIFtKG[xcn3heIlwdg>?	NUexeYtlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0PFI3OzRpPkK1OFgzPjN2PD;hQi=>
TE13	MV;BdI9xfG:\|aYOgRZN{[Xl?	NXP2dnd6OC5\|zszN	MV:yOIg>		NWXrS5VZcW6qaXLpeJMhfGinIHPlcIwheHKxbHnm[ZJifGmxbh?=	M2W5SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NUe5OlY2Lz5{NUW3PVY3PTxxYU6=
TE13	M1vMZWZ2dmO2aX;uJGF{e2G7	NWXXRWY{OC5\|zszN	MnTWNlRp		MWr1dE1z\We3bHH0[ZMhWkGVU1[1RUBt\X[nbB?=	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV5OU[2OUc,OjV3N{m2OlU9N2F-
HEK 293	NGK4SnpHfW6ldHnvckBCe3OjeR?=	MVKwMlfPxE1?	MYWyOIg>	MnHQ[ZRp[W6xbB?=	NWTYXnY6\W6qYX7j[ZMhTU6jQzDhZ4V1gWyjdHnvckBidmRiaX7jdoVie2W\|IFXOZWMh[WK3bnThcoNmKGmwIITo[UB1d3SjbDDj[YxtKGy7c3H0[UBidmRiYYSgeIhmKGOnbHygd5Vz\mGlZR?=	NWTC[\|E1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3PFcxPzlpPkK1O\|g4ODd7PD;hQi=>
MDA-MB-231	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NUXJUWZzOjVvNECwJI5O	NVHMd4xyPDhiaB?=		NHTNPGtKSzVyIH;mJFI3Oy5{bl5CpC=>	NFfTTlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C1OVE6QCd-MkOwOVUyQTh:L3G+
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HepG2	MljRSpVv[3Srb36gZZN{[Xl?				Ml3xWZAuemWpdXzheIlwdiCxZjD0doFve2O{aYD0bY9v[WxiYXP0bZZqfHlib3[gbJVu[W5iQ1zBMVEheHKxbX;0[ZIhemWpaX;uJE0yODV3IITvJE03OmKyIITyZY5{\mWldHXkJIlvKEincFeyJINmdGy\|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NEA:KDBwM{ig{txONg>?	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3NkGwNkc,OjV3NU[xNFI9N2F-
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HL60	MYHBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		M2XaZlI1KGi{cx?=		NXvF[3NLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO\|YYmsJGdKPTBiPTCwMlU6KM7:TT6=	NI\TNIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS1OFkyQCd-Mkm0OVQ6OTh:L3G+
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melanoma B16/BL6	MnHwSpVv[3Srb36gZZN{[Xl?				M4LvbmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBpcXO2b37lJIRm[WOndInsZZNmKCiKRFHDOEkheHKncHHy[YQh\nKxbTDtc5V{\SCvZXzhco9u[SCEMU[vRmw3KGOnbHzzMEBKSzVyIE2gNE4xOiEQvF2u	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
melanoma B16/BL6	NYj5T\|YxTnWwY4Tpc44h[XO\|YYm=				MmnHTY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JIhqe3SxbnWg[IVi[2W2eXzhd4UhPiCycnXwZZJm\CCocn;tJI1wfXOnIH3lcIFvd22jIFKxOk9DVDZiY3XscJMtKEmFNUCgQUAxNjB{ODFOwG0v	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTFyOU[yOkc,OTVzMEm2NlY9N2F-
melanoma B16/BL6	NVTFZY1YTnWwY4Tpc44h[XO\|YYm=				NGTQR5hKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhcGm\|dH;u[UBl\WGlZYT5cIF{\SB6IIDy[ZBiemWmIH\yc40hdW:3c3WgcYVt[W6xbXGgRlE3N0KONjDj[YxteyxiSVO1NEA:KDBwMESg{txONg>?	NGnnTnM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUGwPVYzPid-MUWxNFk3OjZ:L3G+
293T	NXLaNW5JTnWwY4Tpc44h[XO\|YYm=				M{XH[mlvcGmkaYTpc44hd2ZiaIXtZY4hcGm\|dH;u[UBl\WGlZYT5cIF{\SB2IIDy[ZBiemWmIH\yc40hOjl\|VDDj[YxteyxiSVO1NEA:KDBwMEKg{txONg>?	MkLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyNlQoRjF3NEW0NlI1RC:jPh?=
293T	Ml;USpVv[3Srb36gZZN{[Xl?				MY\Jcohq[mm2aX;uJI9nKGi3bXHuJIhqe3SxbnWg[IVi[2W2eXzhd4UhOSCycnXwZZJm\CCocn;tJFI6O1RiY3XscJMtKEmFNUCgQUAxNjB{IN88UU4>	MkS0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2NUSyNlQoRjF3NEW0NlI1RC:jPh?=
293T	NXPDUGR[TnWwY4Tpc44h[XO\|YYm=				NVvBTnVxUW6qaXLpeIlwdiCxZjDoeY1idiCqaYP0c45mKGSnYXPleJlt[XOnIE[gdJJmeGG{ZXSg[pJwdSB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6wNkDPxE1w	NGrXN2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUS1OFIzPCd-MUW0OVQzOjR:L3G+
293T	M3fXVGZ2dmO2aX;uJIF{e2G7				MWPJcohq[mm2aX;uJI9nKGi3bXHuJIhqe3SxbnWg[IVi[2W2eXzhd4UhQCCycnXwZZJm\CCocn;tJFI6O1RiY3XscJMtKEmFNUCgQUAxNjB2IN88UU4>	M3;pXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEW0NlI1Lz5zNUS1OFIzPDxxYU6=
HeLa	NWO1[pBMTnWwY4Tpc44h[XO\|YYm=				M3HG[2lvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IHj1cYFvKGirc4TvcoUh\GWjY3X0fYxie2ViKFjERWMqKG[{b32gTIVN[SClZXzsd{B4cXSqIIP1ZpN1emG2ZTC1ZUwhUUN3MDC9JFAvODV2NTFOwG0v	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR3NkK2O{c,OTV2NU[yOlc9N2F-
HCT116	M1\jUmFvfGmycn;sbYZmemG2aY\lJIF{e2G7				NWq1Uo1rSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBJS1RzMU[gZ4VtdHNuIFnDOVAhRSByLkCwNVMh\|ryPLh?=	M3;YPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUC0PFkxLz5zNkmwOFg6ODxxYU6=
H1299	MnLlRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?				MkLDRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDINVI6QSClZXzsd{whUUN3MDC9JFAvOSEQvF2u	MlrvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MES4PVAoRjF4OUC0PFkxRC:jPh?=
H661	MmewRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?				MmOzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDIOlYyKGOnbHzzMEBKSzVyIE2gNE4{KM7:TT6=	M1;FS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUC0PFkxLz5zNkmwOFg6ODxxYU6=
H661	MYfBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		MXy0PEBpenN?		NXnhUlRRSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBJPjZzIHPlcIx{KGGodHXyJFQ5KGi{czDifUBZXFRiYYPzZZktKEmFNUCgQUAxNjB6NTFOwG0v	M{T5VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkK0O\|c{Lz5zN{[yOFc4OzxxYU6=
WiDr	NELlNmdIem:5dHigbY5pcWKrdHnvckBie3OjeR?=				NGDKXIxKdmirYnn0bY9vKG:oIGPBVFE{OCCvZXTpZZRm\CClZXzsJIdzd3e2aDDpckBpfW2jbjDXbWRzKGOnbHzzMEBKSzVyIE2gNE42Pzd4IN88UU4>	M33QUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkSzNVEzLz5zN{[0N\|EyOjxxYU6=
U251	NEDHfVRHfW6ldHnvckBie3OjeR?=				M1\RUGlvcGmkaYTpc44hd2ZiU1HQNVMxKGmwIG\FS2Yue3SrbYXsZZRm\CCqdX3hckBWOjVzIHPlcIx{KGK7IGDMRXAhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NEA:KDBwNkGxNkDPxE1w	NUfINItURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OFMyOTJpPkG3OlQ{OTF{PD;hQi=>
293T	MlX3SpVv[3Srb36gZZN{[Xl?				MVPJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNzIHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yMEG5JO69VS5?	NGL6dlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{i4NVI{Oid-MUe4PFEzOzJ:L3G+
293T	MoL0SpVv[3Srb36gZZN{[Xl?				MUTJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN2IHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yMEKg{txONg>?	M1HMcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OEixNlMzLz5zN{i4NVI{OjxxYU6=
293T	NHS3TnRHfW6ldHnvckBie3OjeR?=				MnPZTY5pcWKrdHnvckBw\iCvb4Xz[UBJTEGFNjDlfJBz\XO\|ZXSgbY4hOjl\|VDDj[YxteyxiSVO1NEA:KDBwMECyPEDPxE1w	Ml7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd6OEGyN\|IoRjF5OEixNlMzRC:jPh?=
H661	NIjvVFdCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M3vMOVQ5KGi{cx?=		MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFi2OlEh[2WubIOgZYZ1\XJiNEigbJJ{NCCLQ{WwJF0hOC5yOEWg{txONg>?	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzh7N{iyOEc,OTd6OUe4NlQ9N2F-
293T	NHnWfmJHfW6ldHnvckBie3OjeR?=				NE\UbpFKdmirYnn0bY9vKG:oIFjERWMyKGW6cILld5Nm\CCrbjCyPVNVKGOnbHzzMEBKSzVyIE2gNE4yQSEQvF2u	NIrS[5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mwNFkyOSd-MUe5NFA6OTF:L3G+
293T	MX3GeY5kfGmxbjDhd5NigQ>?				MVXJcohq[mm2aX;uJI9nKEiGQVO0JIV5eHKnc4Pl[EBqdiB{OUPUJINmdGy\|LDDJR\|UxKD1iMD6yJO69VS5?	NXf2WmNPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5NFA6OTFpPkG3PVAxQTFzPD;hQi=>
293T	Mn\tSpVv[3Srb36gZZN{[Xl?				M1zsdmlvcGmkaYTpc44hd2ZiSFTBR\|Yh\XiycnXzd4VlKGmwIEK5N3Qh[2WubIOsJGlEPTBiPTCwMlI5KM7:TT6=	NILlVVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mwNFkyOSd-MUe5NFA6OTF:L3G+
NIH3T3	M1zwe2Z2dmO2aX;uJIF{e2G7				MnLJTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDNUBmgHC{ZYPz[YQhcW5iTlnIN3Q{KGOnbHzzMEBKSzVyIE2gNE4xOjFizszNMi=>	NUXpR4E3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5Nlk4QThpPkG3PVI6Pzl6PD;hQi=>
human 293	Mk\1SpVv[3Srb36gZZN{[Xl?				NFq2bpNKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWM1KGW6cILld5Nm\CCrbjCyPVMh[2WubIOsJGlEPTBiPTCwMlA{PCEQvF2u	Mkf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7Mkm3PVgoRjF5OUK5O\|k5RC:jPh?=
mouse 293	M2D6bmZ2dmO2aX;uJIF{e2G7				Mn\ZTY5pcWKrdHnvckBw\iCvb4Xz[UBz\WOxbXLpcoFvfCCKRFHDOkBmgHC{ZYPz[YQhcW5iMkmzJINmdGy\|LDDJR\|UxKD1iMD6wPFEh\|ryPLh?=	NGLoeoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{myPVc6QCd-MUe5Nlk4QTh:L3G+
SKBR3	NYm1[YxpSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				MYPBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFONQmKzJINmdGy\|LDDJR\|UxKD1iMD6wNkDPxE1w	NUfDSpM3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
MM96L	NGfzUoNHfW6ldHnvckBie3OjeR?=				NXv3RYJFUW6qaXLpeIlwdiCxZjDoeY1idiCPTUm2UEBk\WyuczygTWM2OCB;IECuNFMh\|ryPLh?=	Mlz0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEe1OVQoRjF6MkS3OVU1RC:jPh?=
SNU16	M4iwZWZ2dmO2aX;uJIF{e2G7				MYTJcohq[mm2aX;uJI9nKEiGQVOg[pJwdSCqdX3hckBUVlVzNjDj[YxteyxiSVO1NEA:KDBwMEWzJO69VS5?	NYCxfHF3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
A549	MYnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?				MlLKRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCDNUS5JINmdGy\|LDDJR\|UxKD1iMD6wPEDPxE1w	NED6bYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
MKN45	NVO5enA{SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=				MnrYRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPS160OUBk\WyuczygTWM2OCB;IECuNUDPxE1w	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
SQ20B	MV\GeY5kfGmxbjDhd5NigQ>?				NGj1e45KdmirYnn0bY9vKG:oIHj1cYFvKFOTMkDCJINmdGy\|LDDJR\|UxKD1iMD6yJO69VS5?	NETCTIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
neonatal foreskin fibroblasts	Mmm1S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?				MVnHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDu[Y9v[XSjbDDmc5Jme2urbjDmbYJzd2KuYYP0d{Bk\WyuczygTWM2OCB;IECuNkDPxE1w	MlnlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEe1OVQoRjF6MkS3OVU1RC:jPh?=
HeLa	NYDufWE{TnWwY4Tpc44h[XO\|YYm=				M3XYV2lvcGmkaYTpc44hd2ZiSFTBR{Bnem:vIHj1cYFvKEinTHGgZ4VtdHNuIFnDOVAhRSByLkOg{txONg>?	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MM96L/NFF	NE\OeWJHfW6ldHnvckBie3OjeR?=				MXrT[Yxm[3Srdnn0fUBqdmSneDygdoF1cW9ib3[gTWM2OCCob4KgbJVu[W5iTV25Omwh[2WubIOgeI8hUUN3MDDmc5IhcHWvYX6gUmZHKGOnbHzzMEBKSzVyIE2gOk44KM7:TT6=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
293T	M4rtV2Z2dmO2aX;uJIF{e2G7				MlPHTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTDlfJBz\XO\|ZXSgbY4hOjl\|VDDj[YxteyxiSVO1NEA:KDBwMEO2OkDPxE1w	NG\3eXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5O\|gzPid-MUizPVc5OjZ:L3G+
293T	MUfGeY5kfGmxbjDhd5NigQ>?				NUeye3FFUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOEBmgHC{ZYPz[YQhcW5iMkmzWEBk\WyuczygTWM2OCB;IECuNFYzQSEQvF2u	Ml\sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OUe4NlYoRjF6M{m3PFI3RC:jPh?=
293T	MUnGeY5kfGmxbjDhd5NigQ>?				MXvJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN4IHX4dJJme3OnZDDpckAzQTOWIHPlcIx{NCCLQ{WwJF0hOC5yOEOzJO69VS5?	MmPBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OUe4NlYoRjF6M{m3PFI3RC:jPh?=
HeLa	NV3Zd29XTnWwY4Tpc44h[XO\|YYm=				MUDJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNiaX6gTIVN[SClZXzsd{BjgSCobH;1dkBl\SCueYOgZZN{[XluIFnDOVAhRSByLkCwOUDPxE1w	Mk\yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OUe4NlcoRjF6M{m3PFI4RC:jPh?=
S2	MlPaSpVv[3Srb36gZZN{[Xl?				MUnJcohq[mm2aX;uJI9nKFCuYYPtc4RqfW1iZnHsZ4lx[XK3bTDISGFEOSCneIDy[ZN{\WRiaX6gSJJwe2:yaHnsZUBu\Wyjbn;nZZN1\XJiU{KgZ4VtdHNuIFnDOVAhRSByLkCwNFYh\|ryPLh?=	M13oNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7M{G3OFUxLz5zOUOxO\|Q2ODxxYU6=
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HEK293	NEjlPY9EgXSxdH;4bYNqfHliYYPzZZk>		MYq5OkBpenN?		MUTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDISWszQTNiY3XscJMh[W[2ZYKgPVYhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvOTZizszNMi=>	NV24bIFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1OVYyODJpPkK1OVU3OTB{PD;hQi=>
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HepG2	M3T1WGN6fG:2b4jpZ4l1gSCjc4PhfS=>		Mn3pPVYhcHK\|		Ml3KR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgPVYhcHK\|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvQTZizszNMi=>	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTV3NkGwNkc,OjV3NU[xNFI9N2F-
U2OS	NIfY[5dEgXSxdH;4bYNqfHliYYPzZZk>		NYLBUIQyQTZiaILz		NETsXI5EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBWOk:VIHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAyPC57MzFOwG0v	NFnDeYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUW1OlExOid-MkW1OVYyODJ:L3G+
Sf9	NFf4TmVHfW6ldHnvckBie3OjeR?=		M4Xhd\|kxKG2rboO=		NEfpXWFKdmirYnn0bY9vKG:oIH\1cIwhdGWwZ4ToJIh2dWGwIILlZ49u[mmwYX70JG4ufGW{bXnuZYwhT1OWLYTh[4dm\CCKRFHDOkApOSC2bzCxNlE2KHKnc3nkeYV{MSCneIDy[ZN{\WRiaX6gd4Y6KGOnbHzzJJV{cW6pIGLIT{1MMEGlKT3BUWMh[XNic4Xid5Rz[XSnIHnuZ5Vj[XSnZDDmc5IhQTBibXnud{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkCwNVcyPiEQvF2u	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB4MEe2OEc,OjdyNkC3OlQ9N2F-
Sf21	MmXxSpVv[3Srb36gZZN{[Xl?		M2\5SlYxKG2rboO=		MXHJcohq[mm2aX;uJI9nKG[3bHygcIVv\3SqIHj1cYFvKHKnY3;tZolv[W62IFOteIVzdWmwYXygSmxCTy2KaYOteIFo\2WmIFjERWMyKChzIITvJFQ5OiC{ZYPp[JVmeyliZYjwdoV{e2WmIHnuJHNnOjFiY3XscJMhfXOrbnegVmhMNUtqQXOpMWFOSyCjczDzeYJ{fHKjdHWgbY5kfWKjdHXkJIZweiB4MDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeTygTWM2OCB;IECuNFA3PTd2IN88UU4>	NIHhV2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
insect cell	M{X2[2Z2dmO2aX;uJIF{e2G7		M{TiW\|YxKG2rboO=		MofNTY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDoeY1idiC{ZXPvcYJqdmGwdDDDMZRmem2rbnHsJGdUXC22YXfn[YQhUESDQ{KgLFEhfG9iNEi4JJJme2mmdXXzLUBmgHC{ZYPz[YQhcW5iaX7z[YN1KGOnbHzzJJV{cW6pIGLIT{1MMEGlKT3BUWMh[XNic4Xid5Rz[XSnIHnuZ5Vj[XSnZDDmc5IhPjBibXnud{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLkCxNFUyKM7:TT6=	NEm3dHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
insect cell	NYT6ZVVHTnWwY4Tpc44h[XO\|YYm=		Mm\LOlAhdWmwcx?=		NV;sTY9MUW6qaXLpeIlwdiCxZjDmeYxtKGynbnf0bEBpfW2jbjDy[YNwdWKrbnHueEBENXSncn3pcoFtKEircz30ZYdo\WRiSFTBR\|MhMDFidH:gOFI5KHKnc3nkeYV{MS:qdX3hckBz\WOxbXLpcoFvfCCQLYTldo1qdmGuIFfTWE11[WepZXSgUmNQWjJiZYjwdoV{e2WmIHnuJIlve2WldDDj[YxteyC3c3nu[{BTUEtvSzjBZ{kuSU2FIHHzJJN2[nO2cnH0[UBqdmO3YnH0[YQh\m:{IE[wJI1qdnNiYomg[ox2d3Knc3PlcoNmKGG\|c3H5MEBKSzVyIE2gNE4xOTFyNzFOwG0v	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB4MEe2OEc,OjdyNkC3OlQ9N2F-
insect cell	NVHB[YFVTnWwY4Tpc44h[XO\|YYm=				M2r4UmlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiTj30[ZJucW6jbDDIbZMufGGpZ3XkJGhFSUNzMTCoNUB1dyB\|NEegdoV{cWS3ZYOpJIV5eHKnc4Pl[EBqdiCrboPlZ5Qh[2WubIOvZoFkfWyxdnnyeZMh\XiycnXzd4lwdiC\|eYP0[Y0hfXOrbnegVmhMNUtqQXOpMWFOSyCjczDzeYJ{fHKjdHWgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwJF0hOC5yMUGzO{DPxE1w	MnvyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyNkC3OlQoRjJ5ME[wO\|Y1RC:jPh?=
insect cell	NF\1cXJHfW6ldHnvckBie3OjeR?=		M2r0RlYxKG2rboO=		MkT5TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCFLYTldo1qdmGuIFjpd{11[WepZXSgTGRCSzhiKEGgeI8hOzd5IILld4llfWW\|KTDlfJBz\XO\|ZXSgbY4hcW6\|ZXP0JINmdGy\|IIXzbY5oKFKKSz3LLGFkMS2NKFHjLU1CVUNiYYOgd5Vje3S{YYTlJIlv[3WkYYTl[EBnd3JiNkCgcYlveyCkeTDmcJVwemW\|Y3XuZ4Uh[XO\|YYmsJGlEPTBiPTCwMlMxPzRizszNMi=>	NUL3SItURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewOlA4PjRpPkK3NFYxPzZ2PD;hQi=>
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insect cell	MWTGeY5kfGmxbjDhd5NigQ>?		NYHa[YJCPCCqcoO=		M132bGlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR\|Yh\XiycnXzd4VlKGmwIHLhZ5Vtd3[rcoXzJIlv\mWldHXkJIlve2WldDDj[YxteyC3c3nu[{BDSVSFUDDhd{B{fWK\|dILheIUh[W[2ZYKgOEBpenNiYomgWWhRVENvRWPJMW1UN02VIHHuZYx6e2m\|LDDJR\|UxKD1iMD6wNVYh\|ryPLh?=	NXrCcndMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OVA6OjVpPkK3OlUxQTJ3PD;hQi=>
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HEK293T	MlPJSpVv[3Srb36gZZN{[Xl?		MUCzNEBucW6\|		MXnJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEiGQVOxJIV5eHKnc4Pl[EBqdiCKRVuyPVNVKGOnbHzzJJV{cW6pIFHjMWtIVEeNKFHjLU1OS0FiYYOgd5Vje3S{YYTlJIFnfGW{IEOwJI1qdnNiaX6gdJJme2WwY3Wgc4YhTFSWIHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3MDC9JFAvODF7IN88UU4>	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTVzOU[wOEc,Ojl3MUm2NFQ9N2F-
HEK293T	Mn\RSpVv[3Srb36gZZN{[Xl?		M33MUFMxKG2rboO=		MlnyTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDtc5V{\SCKRFHDOkBmgHC{ZYPz[YQhcW5iSFXLNlk{XCClZXzsd{Bkdy2neIDy[ZN{cW6pIH3veZNmKEiGQUKgeZNqdmdiQXOtT2dNT0tqQXOpMW1ESSCjczDzeYJ{fHKjdHWgZYZ1\XJiM{CgcYlveyCrbjDwdoV{\W6lZTDv[kBFXFRiYomg[ox2d3Knc3PlcoNmKGG\|c3H5MEBKSzVyIE2gNE4xOjhizszNMi=>	NHvC[o49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWxPVYxPCd-Mkm1NVk3ODR:L3G+
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insect cell	M4DDfWZ2dmO2aX;uJIF{e2G7				NXzJZ25lUW6qaXLpeIlwdiCxZjDOMZRmem2rbnHsJGdUXC22YXfn[YQhemWlb33ibY5idnRiaIXtZY4hUESDQ{[g[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC\|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR\|UxKD1iMD6wNFg2KM7:TT6=	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN4NUi5Nkc,OzB\|NkW4PVI9N2F-
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insect cell	MXnGeY5kfGmxbjDhd5NigQ>?				M4LQfWlvcGmkaYTpc44hd2ZicnXjc41jcW6jboSgbJVu[W5iSFTBR\|MwVkORUkKg[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|IHnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC\|dXLzeJJifGViYomg[ox2d3Knc3PlcoNmNWKjc3XkJIF{e2G7LDDJR\|UxKD1iMD6wNVI4KM7:TT6=	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODN4NUi5Nkc,OzB\|NkW4PVI9N2F-
insect cell	MYjGeY5kfGmxbjDhd5NigQ>?				MmfHTY5pcWKrdHnvckBw\iCFLYTldo1qdmGuIFfTWE11[WepZXSgdoVkd22kaX7hcpQhcHWvYX6gTGRCSzJiZYjwdoV{e2WmIHnuJIJi[3Wub4\pdpV{KGmwZnXjeIVlKGmwc3XjeEBk\WyuczD1d4lv\yCUSFvLLGFkMUGPQzDhd{B{fWK\|dILheIUh[nliZnz1c5Jme2OnbnPlMYJie2WmIHHzd4F6NCCLQ{WwJF0hOC5yMk[3JO69VS5?	NX7Q[JR6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CzOlU5QTJpPkOwN\|Y2QDl{PD;hQi=>
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insect cell	Mn35SpVv[3Srb36gZZN{[Xl?		NVv4S\|YxPjBibXnudy=>		Mnf5TY5pcWKrdHnvckBw\iCodXzsJIxmdme2aDDy[YNwdWKrbnHueEBpfW2jbjDDMZRmem2rbnHsJGdUXC22YXfn[YQhUESDQ{Kg[ZhxemW\|c3XkJIlvKGKjY4Xsc5ZqenW\|LXnu[oVkfGWmIHnud4VkfCClZXzsd{B2e2mwZzDSTGtMMEGlKVHNR{BieyC\|dXLzeJJifGViYX\0[ZIhPjBibXnud{BjgSCobIXvdolu\XSncjygT4khRSByLkCwN\|U2KM7:TT6=	MlPlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
insect cell	M{D5SmZ2dmO2aX;uJIF{e2G7				NHzaN2NKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JIh2dWGwIFjERWMyOCCneIDy[ZN{\WRiaX6gZoFkfWyxdnnyeZMucW6oZXP0[YQhcW6\|ZXP0JINmdGy\|IIXzbY5oKG[udX;yc4dmdmmlIIDldJRq\GViUljLT2FkKGG\|IIP1ZpN1emG2ZTDifUBndHWxcnnt[ZRmeixiS3mgQUAxNjByNEe2JO69VS5?	M3rNU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
insect cell	M2jTeGZ2dmO2aX;uJIF{e2G7		Ml\rOlAhdWmwcx?=		MWHJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKENvdHXycYlv[WxiSHnzMZRi\2enZDDISGFEQCBqMTD0c{A{PzdicnXzbYR2\XNrIHX4dJJme3OnZDDpckBj[WO3bH;2bZJ2ey2rbn\lZ5Rm\CCrboPlZ5Qh[2WubIOgeZNqdmdiUljLLGFkMUtqQXOpRW1EKGG\|IIP1ZpN1emG2ZTDh[pRmeiB4MDDtbY5{KGK7IH\seY9zcW2ndHXyMEBMcSB;IECuNVQ2KM7:TT6=	Ml\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl3OEm0OFEoRjJ7NUi5OFQyRC:jPh?=
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MCF7	NW[5XWs6TnWwY4Tpc44h[XO\|YYm=	MmPDNE42KHWP	NGO3fIwzPCCqcoO=		MVnJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJG1ETjdiY3XscJMh[XO\|ZYPz[YQh[XNiY3jhcodmKGmwIIT1ZpVtcW5iYXPleJlt[XSrb36gcIV3\WxiYYSgNE42KHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYO=	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ6MEO2N{c,OjJ{OECzOlM9N2F-
MCF7	MkO0SpVv[3Srb36gZZN{[Xl?	MX:wMlUhfU1?	NEm5WoIzPCCqcoO=		M1TOe2lvcGmkaYTpc44hd2ZiSFTBR\|EhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczD1dE1z\We3bHH0bY9vKG:oIHHj[ZR6dGG2ZXSgbIl{fG:wZTDIN{Bxem:2ZXnuJIxmfmWuIHH0JFAvPSC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz	M2naeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MkiwN\|Y{Lz5{MkK4NFM3OzxxYU6=
PC12	MXvGeY5kfGmxbjDhd5NigQ>?	NHjmcFAyOCC3TR?=	NUnMUZJWPDhiaILz		NYGyeo5HUW6qaXLpeIlwdiCxZjDISGFEPiCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDhZ4V1gWyjdHnvckBw\iCqaYP0c45mKEh\|IHH0JFExKHWPIHHmeIVzKDR6IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKGGwYXz5d4l{	NXO3TXVJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5NFU3QDBpPkKzPVA2PjhyPD;hQi=>
PC12	MVvGeY5kfGmxbjDhd5NigQ>?	NILVZ2oyOCC3TR?=	M3\kTlQ5KGi{cx?=		M4HhS2lvcGmkaYTpc44hd2ZiSFTBR\|YhcW5icnH0JHBEOTJiY3XscJMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFteGijLYT1ZpVtcW5iYXPleJlt[XSrb36gZZQhOTBidV2gZYZ1\XJiNEigbJJ{KGK7IGfld5Rmem5iYnzveJRqdmdiYX7hcJl{cXN?	M4Lo[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
PC12	M{nHOWZ2dmO2aX;uJIF{e2G7	NF7DNVgyOCC3TR?=	NIXreZg2KGSjeYO=		M1vPWGlv\HWldHnvckBw\iCwZYXybZRmKG:3dHfyc5d1cCCrbjDyZZQhWENzMjDj[YxteyCjdDCxNEB2VSCjZoTldkA2KGSjeYO=	NFvPZXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{mwOVY5OCd-MkO5NFU3QDB:L3G+
SH-SY5Y	NXv1dHlwTnWwY4Tpc44h[XO\|YYm=	NEH3V5UyOCC3TR?=	NVmxUmpCPSCmYYnz		MmTCTY5lfWO2aX;uJI9nKG6ndYLpeIUhd3W2Z4Lve5RpKGmwIHj1cYFvKFOKLWPZOXkh[2WubIOgZZQhOTBidV2gZYZ1\XJiNTDkZZl{	MoL5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ\|OUC1OlgxRC:jPh?=
A549	M{K1[WFvfGmycn;sbYZmemG2aY\lJIF{e2G7	NGnEOW8xNjRidV2=	MU[yOEBpenN?		MWHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgd4hzfW6tZX6gZY5lKHOqYYLwJJNp[XCnIHH0JFAvPCC3TTDh[pRmeiB{NDDodpMh[nlicHjhd4Uh[2:wdILhd5QhdWmlcn;zZ49xgQ>?	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTh6NEGxNkc,OjV6OESxNVI9N2F-
HeLa	NYTabHI1TnWwY4Tpc44h[XO\|YYm=				NFz1TI9KdmirYnn0bY9vKG:oIFjERWM3KGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOgeJVjfWyrbjDhZ4V1gWyjdHnvckBqdmO3YnH0[YQh\m:{IH;2[ZJvcWeqdDDifUBqdW23bn;mcJVwemW\|Y3XuZ4UhdWmlcn;zZ49xcWNiYX7hcJl{cXN?	M4fkXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NkGxPVE6Lz5{Nk[xNVkyQTxxYU6=
C127-LT	NEfKd\|hHfW6ldHnvckBie3OjeR?=	NIOzU5UzODBibl2=	NXnnbHNVOjRiaILz		M1vYWmlvcGmkaYTpc44hd2ZiSFTBR{BqdiCvb4Xz[UBEOTJ5LVzUJINmdGy\|IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiRVfGVEBmgHC{ZYPzbY9vKGG2IEKwNEBvVSCjZoTldkAzPCCqcoOgZpkh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcIm=	NVTnemR4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVY{PzJpPkK2PVk3Ozd{PD;hQi=>
HeLa nuclear	NHHF[mFHfW6ldHnvckBie3OjeR?=	NIHSbW4yOCC2bzCyOUB2VQ>?	MUSzNEBucW6\|		MV\Dc41x\XSrdHn2[UBqdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hUGWOYTDueYNt\WG{IHPlcIx{KGG2IEGwJJRwKDJ3IIXNJJV{cW6pIFLvZ{1i[2W2eXytcJl{cW6nLVHNR{BieyC\|dXLzeJJifGViYX\0[ZIhOzBibXnud{BjgSCOaX7le4VifmW{LVL1dosheGyxdDDhcoFtgXOrcx?=	NIPQSoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OlM4Oid-Mk[5PVY{PzJ:L3G+
HeLa	MYPGeY5kfGmxbjDhd5NigQ>?	MU[yJJVO	MkS3NlQhcHK\|		M4KxU2lvcGmkaYTpc44hd2ZiSFTBR\|EhcW5iaIXtZY4hUGWOYTDj[YxteyCjc4Pld5Nm\CCjczDofZBmemGlZYT5cIF1cW:wIH;mJIhqe3SxbnWgTFMh[XRiMjD1UUBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM>	NGDRVFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{C2NFc3PCd-MkewOlA4PjR:L3G+
HN13	NXnP[4lMTnWwY4Tpc44h[XO\|YYm=		M1XOZVI1KGi{cx?=		MVHJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTG4yOyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gbIl{fG:wZTDIN{BNgXN7IHHj[ZR6dGG2aX;uJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IHntcZVvd2[udX;y[ZNk\W6lZTDhd5NigQ>?	NHz3NYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUCzNVA3Pid-MkmwN\|ExPjZ:L3G+
HeLaS3	M4nKRmZ2dmO2aX;uJIF{e2G7	NIPZRWoyOCC3TR?=			MVzJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVN[VN\|IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFNMQSCjY3X0fYxifGmxbjDheEAyOCC3TTDt[YF{fXKnZDDpcY1m\GmjdHXsfUBjgSCrbX31co9jdG:2IHHuZYx6e2m\|	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N\|U9N2F-
HeLaS3	M1HGfGZ2dmO2aX;uJIF{e2G7	NFi2WYMyOCC3TR?=	NX7USW9vOTZiaILz		NY\Fd5FKUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHHTN{Bk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCqaYP0c45mKEh\|S{mgZYNmfHmuYYTpc44h[XRiMUCgeW0hfHKnYYTl[EBnd3JiMU[gbJJ{KG[xbHzve4VlKGK7IHPvcZBwfW6mIIfhd4hwfXRiYX7kJI1m[XO3cnXkJJVxKHSxIEK0JIhzeyCkeTDpcY12dm:kbH;0JIFv[Wy7c3nz	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N\|U9N2F-

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	EGFR / STAT3 ; PubMed: 31289542 Oncol Lett Expression of EGFR and STAT3 in PC3 cells treated with TSA. PC3 cells were plated in 6-cm dishes, and 0.25, 0.5 or 1 µM TSA was added for 24 h. DMSO was added into the control group wells. Cells were collected and lysed with lysis buffer and the protein expression analyzed via western blotting. Cyclin D1 / CDK6 / CDK4 / p-RB / RB; PubMed: 31289542 Oncol Lett Expression of cyclin D1, CDK6, CDK4 and RB, and the phosphorylation of RB, was detected in PC3 cells treated with TSA for 24 h. MMP-1 / MMP-3 / MMP-13 / TIMP-1; PubMed: 27431944 Mol Med Rep Chondrocytes were pretreated with increasing concentrations of TSA for 2 h, followed by stimulation with 5 ng/ml IL-1β for 24 h. Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1); PubMed: 27431944 Mol Med Rep Chondrocytes were treated with increasing concentrations of TSA for 24 h. α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2; PubMed: 23802098 Front Oncol Histone acetylation occurs directly at the promoter region of IL-23R. The ChIP assay demonstrates that TSA treatment results in an increase in the acetylation of histone H3 and H4. A549 cells were cultured in the presence or absence of TSA (250 ng/mL) for a period of 24 h. Subsequently, a ChIP assay was performed using the following antibodies; pan acetyl-histone H3 (H3Ac), pan acetyl-histone H4 (H4Ac), acetyl-histone H3 Lys 9 (H3K9Ac), acetyl-histone H3 Lys 9/14 (H3K9/14ac), acetyl-histone H3 Lys 9 phosphoSer10 (H3K9S10), methyl-histone H3 Lys 4 (H3K4Me), di methyl-histone H3 Lys 4 (H3K4Me2), and di methyl-histone H3 Lys 9 (H3K9Me2). Input DNA serves as a positive control as recommended by the manufacturer (Diagenode). A no antibody control was included to test for non-specific binding (UT, untreated; TSA, Trichostatin A).	31289542 27431944 23802098
Immunofluorescence	α-C/EBPβ / α-HP1α ; PubMed: 21122806 Exp Cell Res 3T3-L1 preadipocytes were grown on coverslips for three days and induced to differentiate by treatment with MDI (48 h) in the presence of vehicle (-TSA) or 87 nM Trichostatin A (+TSA). Under these experimental conditions: A-H Cells were fixed and permeabilized in cold methanol, subjected to IIF with the indicated antibodies, and nuclei were counterstained with DAPI. Scale bar, 5 μm. α-HDAC1 ; PubMed: 29045501 PLoS One MCF-7 cells were treated with indicated concentrations of trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels. α-tubulin / acetylated histone 3; PubMed: 27957719 Neurotherapeutics Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775.	21122806 29045501 27957719
Growth inhibition assay	Cell viability (A549 cells); PubMed: 27571418 PLoS One Cytotoxicity of histamine determined by MTT assay in A549 cells. Cell viability (MDA-MB-231 cells); PubMed: 27548148 Int J Mol Sci The human breast cancer cells (MDA-MB-231) were incubated with various concentrations of TSA (0–300 nM) for 24 h. Cell viability was measured by WST-8. Cell viability (PC3 cells); PubMed: 31289542 Oncol Lett PC3 cells were plated in a 96-well plate and treated with different doses of TSA (0, 0.25, 0.5 and 1 µM) for 72 h; an MTT assay was used to measure the effects of TSA on PC3 cell proliferation.	27571418 27548148 31289542

体内研究

Administration of Trichostatin A 按 0.5 mg/kg 剂量处理N-甲基-N-亚硝基脲致癌物诱发的大鼠乳腺癌模型，持续处理4周，具有有效的抗癌活性，即使按按高达5 mg/kg剂量处理也没有任何可测量到的毒性。^[1]Trichostatin A 按10 mg/kg 剂量单独腹腔注射给药非转基因和脊髓性肌萎缩症（SMA）模型鼠，导致乙酰化的 H3 和 H4组蛋白水平提高，也导致活运动神经元(SMN) 基因表达稍微提高。Trichostatin A 每天按10 mg/kg剂量处理SMA模型鼠，促进存活, 减轻体重下降,且增强运动行为。^[5]

激酶实验：^[1]	- 合并体外HDAC活性 : 从每种乳腺癌细胞系(MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51,或 SK-BR-3)中准备全部细胞提取物。在溶于0.1% (v/v)乙醇的不同浓度Trichostatin A存在时，或者使用0.1% (v/v)乙醇作为对照, 20 μL 细胞粗提取物(~2.5 ×10⁵ 个细胞)与 1 μL ~1.5 × 10⁶ cpm [³H]乙酰标记的组蛋白 H4 肽底物(第2-20位NH2-末端残基) 在25^oC下温育60分钟。使用50 μL 1 M HCl/0.16 M 乙酸对每组 200-μL 反应进行淬火，然后使用600 μL 乙酸乙酯进行抽提，通过闪烁计数器测量释放的[³H]乙酸。使用非线性回归曲线拟合抑制数据，绘制合适的剂量反应曲线，而测定IC50。
细胞实验：^[1]	- 合并 Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3 Concentrations: 溶于无水酒精,终浓度为~10 μM Incubation Time: 96小时 Method: 使用不同浓度Trichostatin A处理细胞96小时。处理后,使用sulforhodamine B 比色分析测定细胞增殖。通过台酚蓝染色排除法测定细胞活力。 (Only for Reference)
动物实验：^[1]	- 合并 Animal Models: 携带NMU诱导的肿瘤的近交系(Ludwig/Wistar/Olac)处女鼠 Dosages: ~5 mg/kg/day Administration: 皮下注射 (Only for Reference)

参考文献

- 合并

溶解度 (25°C)

体外	DMSO	23 mg/mL (76.05 mM)
	Water	Insoluble
	Ethanol	Insoluble
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+2% Tween 80+ddH2O	1.5mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Trichostatin A (TSA) SDF

分子量	302.4
化学式	C₁₇H₂₂N₂O₃
CAS号	58880-19-6
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	N/A

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04606771	Recruiting	Drug: Osimertinib + Savolitinib\|Drug: Savolitinib + Placebo	Non-Small Cell Lung Cancer	AstraZeneca	September 28 2020	Phase 2
NCT03901053	Recruiting	Device: Reaktiv AFO\|Device: PhatBrace AFO	Foot Injuries and Disorders	Jason Wilken\|Minneapolis Veterans Affairs Medical Center\|Walter Reed National Military Medical Center\|Henry M. Jackson Foundation for the Advancement of Military Medicine\|Center for Veterans Research and Education\|University of Delaware\|Johns Hopkins Bloomberg School of Public Health\|University of Iowa	February 27 2020	Not Applicable
NCT03887650	Recruiting	Drug: Liposomal Bupivicaine 1.3%\|Drug: Bupivacaine 0.5%	Post-operative Pain\|Total Shoulder Arthroplasty\|Osteoarthritis of the Shoulder\|Pain Management	Hartford Hospital	January 14 2019	Phase 4

技术支持

在订购、运输、储存和使用我们的产品的任何阶段，您遇到的任何问题，均可以通过拨打我们的热线电话400-668-6834，或者技术支持邮箱tech@selleck.cn，直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?
回答：
Our S1045 Trichostatin A (TSA) is R enantiomer.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

选择性强的HDAC抑制剂

RGFP966 : HDAC3-selective, IC50=80 nM.
选择性强的HDAC抑制剂

Rocilinostat (ACY-1215) : HDAC5-selective, IC50=5 nM.
活性最强的HDAC抑制剂

Quisinostat (JNJ-26481585) : HDAC1, IC50=0.11 nM; HDAC2, IC50=0.33 nM.
FDA批准的HDAC抑制剂

Vorinostat (SAHA, MK0683) : Approved by FDA against cutaneous T cell lymphoma.
最新的HDAC抑制剂

RG2833 (RGFP109) ^New : Brain-penetrant HDAC inhibitor with IC50 of 60 nM and 50 nM for HDAC1 and HDAC3, respectively.

研究领域

产品类型

定制您的化合物库

Trichostatin A (TSA)

客户使用Selleck生产的Trichostatin A (TSA)发表文献179篇：

产品安全说明书

HDAC抑制剂选择性比较

生物活性

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

参考文献

溶解度 (25°C)

化学数据 Download Trichostatin A (TSA) SDF

动物体内配方计算器 (澄清溶液)

计算器

摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

连续稀释计算器方程

连续稀释

计算结果

分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-01-06)

技术支持

常见问题及建议解决方法

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

相关HDAC产品