Trichostatin A (TSA)

For research use only. Not for use in humans.

目录号:S1045 中文名称:曲古柳菌素A

Trichostatin A (TSA) Chemical Structure

CAS No. 58880-19-6

Trichostatin A (TSA)是一种HDAC抑制剂,无细胞试验中IC50为1.8 nM左右。

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客户使用Selleck生产的Trichostatin A (TSA)发表文献166篇:

产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述 Trichostatin A (TSA)是一种HDAC抑制剂,无细胞试验中IC50为1.8 nM左右。
靶点
HDAC [1]
(Cell-free assay)
~1.8 nM
体外研究

Trichostatin A 抑制八种乳腺癌细胞系,包括MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3增殖,平均IC50为124.4 nM (范围为26.4-308.1 nM),作用于表达ERα的细胞系比作用于表达 ERα阴性的细胞系更有效。Trichostatin A作用于全部乳腺癌细胞系,抑制HDAC活性,平均IC50为 2.4 nM (范围为0.6-2.6 nM), 产生显著的组蛋白 H4高度乙酰化。[1]与 Trapoxin (TPX) 和 Chlamydocin 有效抑制HDAC1 或 HDAC4而不是 HDAC6不同,Trichostatin A 抑制这些HDACs,且抑制程度相似, IC50 分别为6 nM, 38 nM, 和8.6 nM。[2] Trichostatin A (100 ng/mL) 作用于MIA PaCa-2细胞,通过招募p300和 PCAF进入Sp1-NF-Y HDAC复合体,而诱导转化生长因子βⅡ型受体(TβRII) 表达,复合体与TβRII 启动子的DNA片段结合,伴随着Sp1乙酰化,且与复合体相关的HDAC数理全部降低。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HeLa NXrZTGJOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTyNVAwOzBxNUCgcm0> Mo\kO|IhcA>? NFTkXGZFVVOR NYjidVF5cW6lcnXhd4V{KHSqZTDueY1j\XJib3[gUW1RKCkQlN8ocUkhdG:|czDj[YxteyCmb4PlJIRmeGWwZHXueIx6 MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF4NUe0PEc,OjNzNkW3OFg9N2F-
HeLa NIH0fZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV64fJdUOjBibl2= NXnTUW52PzJiaB?= M{L6[mROW09? NGX5bGhKSzVyIH;mJFIxdk1? MlHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNkW3OFgoRjJ|MU[1O|Q5RC:jPh?=
HeLa NGX2R|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rteVQxKG6P M3;0elQ5KGh? M{LpcGROW09? NFTmTohKSzVyIH;mJFQxdk1? NWmyUIdoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlU4PDhpPkKzNVY2PzR6PD;hQi=>
HeLa MojES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUjBUlIyOTByIH7N NUG4VphlOjRiaB?= NG[xbGlFVVOR NX;MPJhYUUN3MDDv[kAyODCwTR?= Mn3rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzNkW3OFgoRjJ|MU[1O|Q5RC:jPh?=
Caco-2  NHi5OlRHfW6ldHnvckBCe3OjeR?= MVSxJOK2VcLi NYD4fJc3OjRiaB?= NHPFR4hl\WO{ZXHz[ZMhW0WUVDDwdo91\WmwIHX4dJJme3Orb36= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF7NUC3NEc,OjNzOUWwO|A9N2F-
HTK MkHySpVv[3Srb36gRZN{[Xl? MVuxNFAuQDBybl2= MUG3NkBp NWex[G92[myxY3vzJHRITi4QsvMAl2lv\HWlZXSgVm9UKGGwZDDINm8zyqCDY3P1cZVt[XSrb36= NFXwfZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K4OFAxOid-MkOyPFQxODJ:L3G+
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H1299  NETCSotHfW6ldHnvckBCe3OjeR?= M3rZdlAvOzQkgKOxxsDDvU1? M4S0N|I1NzR6IHi= M{DScmROW09? M1P5XYlv[3KnYYPld{BGNWOjZHjldolvKHC{b4TlbY4hdGW4ZXzzJIRwe2ViZHXw[Y5l\W62bIm= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR4MUm3OUc,OjN2NkG5O|U9N2F-
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A2780 Mk\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVOwMlA2NTJizszN MlTPNVAwOjRxNEigbC=> MUPEUXNQ NVexN2pGdWWmaXH0[ZMh\3Kxd4ToJIFzemW|dDDpckBiKGOxbnPlcpRz[XSrb36tJIFv\CC2aX3lMYRmeGWwZHXueEBu[W6wZYK= NYjieHNNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNlM5ODFpPkK0NlI{QDBzPD;hQi=>
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HT29  M2S0[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4XBbVE5OCCwTR?= NXriSJd7PzJiaB?= MnfwTWM2OCCxZjCxPFAhdk1? NXntNXBrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSzOlgzPjVpPkK0N|Y5OjZ3PD;hQi=>
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U87 GBM NHfKXmRHfW6ldHnvckBCe3OjeR?= MXmxNFAwPTByIF7t NYXkRWtbPDhiaB?= MkjBNVAxLSCndHjhco9t NH;zU2tKdmS3Y3XzJHNmdmW|Y3XuZ4UuVGmtZTDBcJRmemG2aX;ud{BqdiCQdXPs[YFzKE2xcoDoc4xw\3l? NYjZRmJqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS0OlQ5PDFpPkK0OFY1QDRzPD;hQi=>
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HMEC-1  NU\aZ4VCTnWwY4Tpc44hSXO|YYm= NYHkdXBuOzBybl2= MofDNlRp NIL0b3dqdmO{ZXHz[ZMh\XiycnXzd4lwdiCxZtMgWmVITlJ|wrDtVm5C NFn6eJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEexNFY{OSd-MkS3NVA3OzF:L3G+
HeLa  Mk\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYe1Xm5rOs7:TR?= NIPoNZk1QGh? NUXGXVRJ\GWlcnXhd4V{KGOnbHygeoli[mmuaYT5JIFjd3W2IEK1KS=> M3G0TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OES2NVM2Lz5{NEi0OlE{PTxxYU6=
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MDA-MB-231 MnrRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2K3U|AuPjBybl2= MXKyOIg> MmnWSG1UVw>? NHf1UmJKSzVyIH;mJFExOG6P Ml76QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVzOUK3NlEoRjJ3MUmyO|IyRC:jPh?=
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SW480  MoO4SpVv[3Srb36gRZN{[Xl? MmXnNE4y|ryP NFX2Ro81QGh? M1ywT2ROW09? NYjuOI4zcW6mdXPld{BqdmO{ZXHz[UBw\iCKRFHDNUBidmRiSFTBR|Ihd25iU3z1[{Bo\W6nczDwdo9ud3Snch?= NWjtfFM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0N|Q6QTdpPkK1OFM1QTl5PD;hQi=>
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MCF7 M2W4eGZ2dmO2aX;uJIF{e2G7 M1q1dVAvPSC3TR?= NXX6WGhqOjRiaILz MoTrTY5pcWKrdHnvckBw\iCKRFHDNUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IIXwMZJm\3WuYYTpc44hd2ZiYXPleJlt[XSnZDDobZN1d26nIFizJJBzd3SnaX6gcIV3\WxiYYSgNE42KHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NYnlUGNsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyPFA{PjNpPkKyNlgxOzZ|PD;hQi=>
PC12 NVvOfXlkTnWwY4Tpc44h[XO|YYm= NFXyXIIyOCC3TR?= MXW0PEBpenN? M1v1U2lvcGmkaYTpc44hd2ZiSFTBR|YhcW5icnH0JHBEOTJiY3XscJMh[XO|ZYPz[YQh[XNiYXPleJlt[XSrb36gc4YhcGm|dH;u[UBJOyCjdDCxNEB2VSCjZoTldkA1QCCqcoOgZpkhX2W|dHXyckBjdG:2dHnu[{BidmGueYPpdy=> MlGwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
PC12 MW\GeY5kfGmxbjDhd5NigQ>? M3Lt[VExKHWP MXW0PEBpenN? MUDJcohq[mm2aX;uJI9nKEiGQVO2JIlvKHKjdDDQR|EzKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDhcJBp[S22dXL1cIlvKGGlZYT5cIF1cW:wIHH0JFExKHWPIHHmeIVzKDR6IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKGGwYXz5d4l{ Ml7OQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
PC12 NYjre5R6TnWwY4Tpc44h[XO|YYm= M1rj[|ExKHWP MkfvOUBl[Xm| NHXzWWNKdmS3Y4Tpc44hd2ZibnX1dol1\SCxdYTndo94fGhiaX6gdoF1KFCFMUKgZ4VtdHNiYYSgNVAhfU1iYX\0[ZIhPSCmYYnz MnnMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7MEW2PFAoRjJ|OUC1OlgxRC:jPh?=
SH-SY5Y NWHBW217TnWwY4Tpc44h[XO|YYm= MVexNEB2VQ>? M4nkc|Uh\GG7cx?= MUDJcoR2[3Srb36gc4YhdmW3cnn0[UBwfXSpcn;3eIghcW5iaIXtZY4hW0hvU2m1XUBk\WyuczDheEAyOCC3TTDh[pRmeiB3IHThfZM> M2XqVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUC1OlgxLz5{M{mwOVY5ODxxYU6=
A549 Mnv6RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NVXRRnZROC52IIXN MViyOEBpenN? MkPZRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHzd4V{e2WmIHHzJJNpenWwa3XuJIFv\CC|aHHydEB{cGGyZTDheEAxNjRidV2gZYZ1\XJiMkSgbJJ{KGK7IIDoZZNmKGOxboTyZZN1KG2rY4Lvd4NweHl? NWnH[5RXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4PFQyOTJpPkK1PFg1OTF{PD;hQi=>
HeLa M1nuU2Z2dmO2aX;uJIF{e2G7 NVrj[nl2UW6qaXLpeIlwdiCxZjDISGFEPiCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJJR2[nWuaX6gZYNmfHmuYYTpc44hcW6ldXLheIVlKG[xcjDveoVzdmmpaISgZpkhcW2vdX7v[ox2d3Knc3PlcoNmKG2rY4Lvd4NweGmlIHHuZYx6e2m| NVn3TJBJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2NVE6OTlpPkK2OlEyQTF7PD;hQi=>
C127-LT NF;MXXFHfW6ldHnvckBie3OjeR?= NYDse3JuOjByIH7N NEjpV5IzPCCqcoO= M4nOZWlvcGmkaYTpc44hd2ZiSFTBR{BqdiCvb4Xz[UBEOTJ5LVzUJINmdGy|IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiRVfGVEBmgHC{ZYPzbY9vKGG2IEKwNEBvVSCjZoTldkAzPCCqcoOgZpkh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcIm= NUTHbYRoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[5PVY{PzJpPkK2PVk3Ozd{PD;hQi=>
HeLa nuclear NVfvdVc1TnWwY4Tpc44h[XO|YYm= NGDwbmMyOCC2bzCyOUB2VQ>? NVj5fow3OzBibXnudy=> Ml6wR49ueGW2aYTpeoUhcW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEinTHGgcpVkdGWjcjDj[YxteyCjdDCxNEB1dyB{NTD1UUB2e2mwZzDCc4Mu[WOndInsMYx6e2mwZT3BUWMh[XNic4Xid5Rz[XSnIHHmeIVzKDNyIH3pcpMh[nliTHnu[Zdm[X[ncj3CeZJsKHCub4SgZY5idHm|aYO= M13NNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUm2N|czLz5{Nkm5OlM4OjxxYU6=
HeLa MoPQSpVv[3Srb36gZZN{[Xl? MXWyJJVO MYmyOEBpenN? NXm3Z3JiUW6qaXLpeIlwdiCxZjDISGFEOSCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJIh6eGW{YXPleJlt[XSrb36gc4YhcGm|dH;u[UBJOyCjdDCyJJVOKGmwY4XiZZRm\CCob4KgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? M1vwR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5ME[wO|Y1Lz5{N{C2NFc3PDxxYU6=
HN13 NWC0VHJRTnWwY4Tpc44h[XO|YYm= NHz3eG4zPCCqcoO= MljLTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhPOTNiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIhqe3SxbnWgTFMhVHm|OTDhZ4V1gWyjdHnvckBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDpcY12dm:obIXvdoV{[2WwY3WgZZN{[Xl? MoTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlyM{GwOlYoRjJ7MEOxNFY3RC:jPh?=
HeLaS3 Mmr0SpVv[3Srb36gZZN{[Xl? NVLNcmhJOTBidV2= MlziTY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJGhmVGGVMzDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBpcXO2b37lJGg{UzliYXPleJlt[XSrb36gZZQhOTBidV2gcYVie3W{ZXSgbY1u\WSrYYTlcJkh[nliaX3teY5w[myxdDDhcoFtgXOrcx?= Ml3BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{NUi1N|UoRjNyMkW4OVM2RC:jPh?=
HeLaS3 MnPzSpVv[3Srb36gZZN{[Xl? M1fLelExKHWP NEezcmoyPiCqcoO= MYrJcohq[mm2aX;uJI9nKEiGQVOgbY4hcHWvYX6gTIVN[VN|IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFNMQSCjY3X0fYxifGmxbjDheEAyOCC3TTD0doVifGWmIH\vdkAyPiCqcoOg[o9tdG:5ZXSgZpkh[2:vcH;1coQhf2G|aH;1eEBidmRibXXhd5Vz\WRidYCgeI8hOjRiaILzJIJ6KGmvbYXuc4Jtd3RiYX7hcJl{cXN? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJ3OEWzOUc,OzB{NUi1N|U9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
EGFR / STAT3 ; 

PubMed: 31289542     


Expression of EGFR and STAT3 in PC3 cells treated with TSA. PC3 cells were plated in 6-cm dishes, and 0.25, 0.5 or 1 µM TSA was added for 24 h. DMSO was added into the control group wells. Cells were collected and lysed with lysis buffer and the protein expression analyzed via western blotting.

Cyclin D1 / CDK6 / CDK4 / p-RB / RB; 

PubMed: 31289542     


Expression of cyclin D1, CDK6, CDK4 and RB, and the phosphorylation of RB, was detected in PC3 cells treated with TSA for 24 h. 

MMP-1 / MMP-3 / MMP-13 / TIMP-1; 

PubMed: 27431944     


Chondrocytes were pretreated with increasing concentrations of TSA for 2 h, followed by stimulation with 5 ng/ml IL-1β for 24 h.

Acetyl-H3 (Lys27) / H3 (96C10) / Acetyl-H4(Lys8) / H4(L64C1); 

PubMed: 27431944     


Chondrocytes were treated with increasing concentrations of TSA for 24 h.

α-H3Ac / α-H4Ac / α-H3K9Ac / α-H3K9 S10 / α-H3K4 me / α-H3K4 me2 / α-H3K9 me2; 

PubMed: 23802098     


Histone acetylation occurs directly at the promoter region of IL-23R. The ChIP assay demonstrates that TSA treatment results in an increase in the acetylation of histone H3 and H4. A549 cells were cultured in the presence or absence of TSA (250 ng/mL) for a period of 24 h. Subsequently, a ChIP assay was performed using the following antibodies; pan acetyl-histone H3 (H3Ac), pan acetyl-histone H4 (H4Ac), acetyl-histone H3 Lys 9 (H3K9Ac), acetyl-histone H3 Lys 9/14 (H3K9/14ac), acetyl-histone H3 Lys 9 phosphoSer10 (H3K9S10), methyl-histone H3 Lys 4 (H3K4Me), di methyl-histone H3 Lys 4 (H3K4Me2), and di methyl-histone H3 Lys 9 (H3K9Me2). Input DNA serves as a positive control as recommended by the manufacturer (Diagenode). A no antibody control was included to test for non-specific binding (UT, untreated; TSA, Trichostatin A).

31289542 27431944 23802098
Immunofluorescence
α-C/EBPβ / α-HP1α ; 

PubMed: 21122806     


3T3-L1 preadipocytes were grown on coverslips for three days and induced to differentiate by treatment with MDI (48 h) in the presence of vehicle (-TSA) or 87 nM Trichostatin A (+TSA). Under these experimental conditions: A-H Cells were fixed and permeabilized in cold methanol, subjected to IIF with the indicated antibodies, and nuclei were counterstained with DAPI. Scale bar, 5 μm. 

α-HDAC1 ; 

PubMed: 29045501     


MCF-7 cells were treated with indicated concentrations of trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels.

α-tubulin / acetylated histone 3; 

PubMed: 27957719     


Immunocytochemistry for acetylated α-tubulin (red) and acetylated histone 3 (green) on N2a cells treated with 1 μM TSA, ACY-738, and ACY-775.

21122806 29045501 27957719
Growth inhibition assay
Cell viability (A549 cells); 

PubMed: 27571418     


Cytotoxicity of histamine determined by MTT assay in A549 cells.

Cell viability (MDA-MB-231 cells); 

PubMed: 27548148     


The human breast cancer cells (MDA-MB-231) were incubated with various concentrations of TSA (0–300 nM) for 24 h. Cell viability was measured by WST-8.

Cell viability (PC3 cells); 

PubMed: 31289542     


PC3 cells were plated in a 96-well plate and treated with different doses of TSA (0, 0.25, 0.5 and 1 µM) for 72 h; an MTT assay was used to measure the effects of TSA on PC3 cell proliferation.

27571418 27548148 31289542
体内研究 Administration of Trichostatin A 按 0.5 mg/kg 剂量处理N-甲基-N-亚硝基脲致癌物诱发的大鼠乳腺癌模型,持续处理4周,具有有效的抗癌活性,即使按按高达5 mg/kg剂量处理也没有任何可测量到的毒性。[1]Trichostatin A 按10 mg/kg 剂量单独腹腔注射给药非转基因和脊髓性肌萎缩症(SMA)模型鼠,导致乙酰化的 H3 和 H4组蛋白水平提高,也导致活运动神经元(SMN) 基因表达稍微提高。Trichostatin A 每天按10 mg/kg剂量处理SMA模型鼠,促进存活, 减轻体重下降,且增强运动行为。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

体外HDAC活性 :

从每种乳腺癌细胞系(MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51,或 SK-BR-3)中准备全部细胞提取物。在溶于0.1% (v/v)乙醇的不同浓度Trichostatin A存在时,或者使用0.1% (v/v)乙醇作为对照, 20 μL 细胞粗提取物(~2.5 ×105 个细胞)与 1 μL ~1.5 × 106 cpm [3H]乙酰标记的组蛋白 H4 肽底物(第2-20位NH2-末端残基) 在25oC下温育60分钟。使用50 μL 1 M HCl/0.16 M 乙酸对每组 200-μL 反应进行淬火,然后使用600 μL 乙酸乙酯进行抽提,通过闪烁计数器测量释放的[3H]乙酸。使用非线性回归曲线拟合抑制数据,绘制合适的剂量反应曲线,而测定IC50。
细胞实验:[1]
- 合并
  • Cell lines: MCF-7, T-47D, ZR-75-1, BT-474, MDA-MB-231, MDA-MB-453, CAL 51, 和 SK-BR-3
  • Concentrations: 溶于无水酒精,终浓度为~10 μM
  • Incubation Time: 96小时
  • Method: 使用不同浓度Trichostatin A处理细胞96小时。处理后,使用sulforhodamine B 比色分析测定细胞增殖。通过台酚蓝染色排除法测定细胞活力。
    (Only for Reference)
动物实验:[1]
- 合并
  • Animal Models: 携带NMU诱导的肿瘤的近交系(Ludwig/Wistar/Olac)处女鼠
  • Dosages: ~5 mg/kg/day
  • Administration: 皮下注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 23 mg/mL (76.05 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
2% DMSO+30% PEG 300+2% Tween 80+ddH2O
3mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 302.4
化学式

C17H22N2O3

CAS号 58880-19-6
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04606771 Recruiting Drug: Osimertinib + Savolitinib|Drug: Savolitinib + Placebo Non-Small Cell Lung Cancer AstraZeneca September 28 2020 Phase 2
NCT03901053 Recruiting Device: Reaktiv AFO|Device: PhatBrace AFO Foot Injuries and Disorders Jason Wilken|Minneapolis Veterans Affairs Medical Center|Walter Reed National Military Medical Center|Henry M. Jackson Foundation for the Advancement of Military Medicine|Center for Veterans Research and Education|University of Delaware|Johns Hopkins Bloomberg School of Public Health|University of Iowa February 27 2020 Not Applicable
NCT03887650 Recruiting Drug: Liposomal Bupivicaine 1.3%|Drug: Bupivacaine 0.5% Post-operative Pain|Total Shoulder Arthroplasty|Osteoarthritis of the Shoulder|Pain Management Hartford Hospital January 14 2019 Phase 4

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    I would like to obtain the enantiomers of TSA, as separate chemicals: R-TSA and S-TSA. Do you have any ideas?

  • 回答:

    Our S1045 Trichostatin A (TSA) is R enantiomer.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

相关HDAC产品

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID