Tanespimycin (17-AAG)

For research use only. Not for use in humans.

目录号：S1141 别名： CP127374, NSC-330507, KOS 953 中文名称：坦螺旋霉素

Tanespimycin (17-AAG) Chemical Structure

CAS No. 75747-14-7

Tanespimycin (17-AAG, CP127374, NSC-330507, KOS 953) 是一种有效的HSP90抑制剂，无细胞试验中IC50为5 nM，作用于来自肿瘤细胞的HSP90比作用于来自正常细胞HSP90结合亲和力高100倍。Tanespimycin (17-AAG)可诱导细胞凋亡、坏死、自噬和线粒体自噬。Phase 3。

规格

价格

库存

购买数量

10mM (1mL in DMSO)	RMB 2350.91	现货
5mg	RMB 795.18	现货
25mg	RMB 2432.97	现货
100mg	RMB 6527.43	现货
200mg	RMB 10950.03	现货
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客户使用Selleck生产的Tanespimycin (17-AAG)发表文献84篇：

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Cancer Cell, 2019, 36(2):179-193

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Sci China Life Sci, 2021, 10.1007/s11427-020-1860-2

PubMed: 33439458 ( click the link to review the publication )

Cell Rep, 2021, 34(6):108726

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Nat Commun, 2020, 29;11(1):2086

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Cell Rep, 2020, 33(7):108397

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Cancers (Basel), 2020, 7;12(4)

PubMed: 32272809 ( click the link to review the publication )

Mol Cell Biol, 2020,

PubMed: 32868290 ( click the link to review the publication )

Mol Cell Biol, 2020, MCB.00473-20

PubMed: 33139492 ( click the link to review the publication )

Phytomedicine, 2020,

PubMed: 32896707 ( click the link to review the publication )

Eur J Cell Biol, 2020, 100(1):151148

PubMed: 33388604 ( click the link to review the publication )

Drug Des Devel Ther, 2020, 14:4605-4612

PubMed: 33149557 ( click the link to review the publication )

G3 (Bethesda), 2020, 4;10(5):1585-1597

PubMed: 32265286 ( click the link to review the publication )

Mol Cell, 2019, 75(4):823-834

PubMed: 31302001 ( click the link to review the publication )

Nat Commun, 2019, 10(1):5043

PubMed: 31695040 ( click the link to review the publication )

Cell Syst, 2019, 8(2):97-108

PubMed: 30797775 ( click the link to review the publication )

Cell Rep, 2019, 27(11):3331-3344

PubMed: 31189115 ( click the link to review the publication )

Cell Death Dis, 2019, 10(10):737

PubMed: 31570706 ( click the link to review the publication )

Cells, 2019, 8(8)

PubMed: 31370342 ( click the link to review the publication )

Front Cell Dev Biol, 2019, 7:204

PubMed: 31608279 ( click the link to review the publication )

Oxid Med Cell Longev, 2019, 2019:2050183

PubMed: 31178953 ( click the link to review the publication )

J Cell Physiol, 2019, 10.1002/jcp.29283

PubMed: 31595984 ( click the link to review the publication )

Cancer Biol Med, 2019, 16(2):220-233

PubMed: 31516744 ( click the link to review the publication )

Sci Rep, 2019, 9(1):5637

PubMed: 30948782 ( click the link to review the publication )
Int J Biol Sci, 2019, 15(2):312-324

PubMed: 30745823 ( click the link to review the publication )

Toxicol Appl Pharmacol, 2019, 371:63-73

PubMed: 30953615 ( click the link to review the publication )

BMC Cancer, 2019, 19(1):102

PubMed: 30678647 ( click the link to review the publication )

Exp Eye Res, 2019, 189:107821

PubMed: 31589841 ( click the link to review the publication )

Mol Cell Biol, 2019, 39(4)e00375-18

PubMed: 30455251 ( click the link to review the publication )

J Natl Cancer Inst, 2018, 110(4):390-399

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Cell Death Dis, 2018, 9(10):944

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Cell Physiol Biochem, 2018, 47(6):2199-2215

PubMed: 29975948 ( click the link to review the publication )

Mol Cancer Ther, 2018, 17(5):1051-1060

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Mol Oncol, 2018,

PubMed: 29797762 ( click the link to review the publication )

J Cell Biochem, 2018, 10.1002/jcb.27397

PubMed: 30378153 ( click the link to review the publication )

Mycoses, 2018, 61(11):853-856

PubMed: 29998564 ( click the link to review the publication )

Eur J Med Chem, 2018, 158:548-558

PubMed: 30243156 ( click the link to review the publication )

F1000Res, 2018, 7:1906

PubMed: 30881689 ( click the link to review the publication )

EMBO J, 2017, 36(5):646-663

PubMed: 28093501 ( click the link to review the publication )

ACS Photonics, 2017, 5(2):487-494

PubMed: 29805987 ( click the link to review the publication )

Br J Pharmacol, 2017, 174(5):409-423

PubMed: 28052321 ( click the link to review the publication )

Cell Mol Life Sci, 2017, 74(3):543-554

PubMed: 27592300 ( click the link to review the publication )

Mol Cancer Ther, 2017,

PubMed: 28619760 ( click the link to review the publication )

Hum Mol Genet, 2017, 26(22):4465-4480

PubMed: 28973376 ( click the link to review the publication )

Sci Rep, 2017, 7(1):4546

PubMed: 28674407 ( click the link to review the publication )

Toxicol Appl Pharmacol, 2017, 329:282-292

PubMed: 28624441 ( click the link to review the publication )

Am J Transl Res, 2017, 9(11):4945-4953

PubMed: 29218092 ( click the link to review the publication )

Onco Targets Ther, 2017, 10:2219-2226

PubMed: 28458565 ( click the link to review the publication )
SLAS Discov, 2017, 22(5):507-515

PubMed: 28324660 ( click the link to review the publication )

Cell, 2017, 170(5):845-859

PubMed: 28823557 ( click the link to review the publication )

Medchemcomm, 2017, 8(1):88-95

PubMed: 28670440 ( click the link to review the publication )

Oncotarget, 2017, 8(62):105372-105382

PubMed: 29285257 ( click the link to review the publication )

Nat Commun, 2016, 7:13122

PubMed: 27959342 ( click the link to review the publication )

Cancer Res, 2016, 76(4):965-73

PubMed: 26676744 ( click the link to review the publication )

Oncogene, 2016,

PubMed: 26804171 ( click the link to review the publication )

Oncogene, 2016, 35(29):3854-3865

PubMed: 26657151 ( click the link to review the publication )

Cell Death Dis, 2016, 7:e2089

PubMed: 26866270 ( click the link to review the publication )

Mol Pharm, 2016, 13(11):3724-3735

PubMed: 27653969 ( click the link to review the publication )

Biochem J, 2016, 473(12):1733-44

PubMed: 27059856 ( click the link to review the publication )

Medicinal Chemistry Research, 2016, 10.1007/s00044-016-1553-7

PubMed: ( click the link to review the publication )

Stem Cells, 2015, 33(9):2738-47

PubMed: 26077647 ( click the link to review the publication )

Cancer Biol Ther, 2015,

PubMed: 25692408 ( click the link to review the publication )

Oncotarget, 2015, 6(17):14796-813

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Oncotarget, 2015, 51: S659-S660

PubMed: ( click the link to review the publication )

Mol Cancer, 2014, 15:30

PubMed: 24927996 ( click the link to review the publication )

ACS Chem Biol, 2014, 9(10):2237-41

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Exp Gerontol, 2014, 52:55-69

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PLoS One, 2014, 9(10):e111146

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Insect Mol Biol, 2014, 23(6):720-32

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Julius Maximilians Universit, 2014, Kumari G

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Oncotarget, 2014, 5(12):4269-82

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Assay Drug Dev Technol, 2014, 12(9-10):514-26

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Mol Cell, 2013, 50(3):368-78

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Biochim Biophys Acta, 2013, 1830(11):5184-92

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Mol Cell Biol, 2013, 33(12):2375-87

PubMed: 23572559 ( click the link to review the publication )

Am J Respir Cell Mol Biol, 2013, 10.1165/rcmb.2013-0214OC

PubMed: 24303801 ( click the link to review the publication )

Sci Transl Med, 2012, 4(120):120ra17

PubMed: 22277784 ( click the link to review the publication )

J Exp Med, 2012, 209(2):259-73

PubMed: 22271575 ( click the link to review the publication )

Biochim Biophys Acta, 2012, 1823(4):911-9

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Eukaryot Cell, 2012, 11(11):1324-32

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Age (Dordr), 2012, 35(3):549-62

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PubMed: 21502504 ( click the link to review the publication )

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PubMed: 21553813 ( click the link to review the publication )

产品安全说明书

HSP (HSP90)抑制剂选择性比较

生物活性

产品描述

特性

17-AAG对正常细胞的毒性很小。

靶点

HSP90 ^[1]
(Cell-free assay)

5 nM

体外研究

17-AAG是格尔德霉素类似物, 但是与过量表达HER-2的癌细胞 (BT474, N87, SKOV3 和SKBR3)或BT474乳腺细胞衍生的Hsp90亲和力高100多倍，IC50为5-6 nM, 而作用于正常人类原代细胞时，IC50为400-943 nM, 因为肿瘤Hsp90 处于多伴复合体状态，具有高ATP酶活性，而正常细胞Hsp90 处于潜在未结合状态, 且直接与那些细胞中17-AAG毒性相关。^[1]17-AAG 引起HER2, HER3, Akt，突变和野生型雄激素受体(AR)降解，导致前列腺细胞如LNCaP, LAPC-4, DU-145,和PC-3中RB依赖的 G1 期生长停顿，IC50为25-45 nM。^[2]除了诱导改变野生型BCR-ABL的Ba/F3细胞凋亡, IC50为5.2 μM, 17-AAG还通过诱导野生型和突变型 BCR-ABL蛋白降解，而诱导改变抗 Imatinib Mesylate的 T315I和 E255K BCR-ABL突变型细胞生长，IC50分别为2.3 μM 和 1.0 μM。^[3]

Cell Data

Cell Lines	Assay Type	Activity Description	PMID
A549	NFr6dHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NYD4WJA1UUN3ME2wMlAxODNyMzFOwG0>	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
IST-MEL1	NFTYdnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M13lc2lEPTB;MD6wNFA1ODdizszN	Ml3sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
NCI-SNU-1	MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4rkd2lEPTB;MD6wNFIxPyEQvF2=	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
FADU	MnzSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXS3OYM3UUN3ME2wMlAxOjB7IN88US=>	NEGwSXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
C32	M3rre2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NG\mXFdKSzVyPUCuNFA{ODRizszN	MlrEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
D-566MG	M{LxNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYDuUIc6UUN3ME2wMlAxOzlizszN	NFzpcmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
LXF-289	M2LSbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M2HqSmlEPTB;MD6wNFQyQSEQvF2=	NXLNfGNMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
HGC-27	MnvGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Ml;ITWM2OD1yLkCwOVEh\|ryP	NYLFcpJxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
RPMI-7951	NHq0VYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MkT4TWM2OD1yLkCwO\|E4KM7:TR?=	NVvhPJBvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
HSC-3	NWjpblBCT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NFjqe2FKSzVyPUCuNFA6OjlizszN	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
MIA-PaCa-2	MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MYLJR\|UxRTBwMEGwNFkh\|ryP	MkK3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
KS-1	Mo\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M{DObGlEPTB;MD6wNVM2PSEQvF2=	NWiz[4MzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
CAL-51	NI\3SXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M2W2SmlEPTB;MD6wNVQxPSEQvF2=	NWLSUpNsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
MDA-MB-361	NH7vNYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MW\JR\|UxRTBwMEG0PVUh\|ryP	M3;BfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
TI-73	NELRPIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Ml;pTWM2OD1yLkCxO\|k3KM7:TR?=	MoTnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
AGS	NFjZR\|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mk\sTWM2OD1yLkCxPVM5KM7:TR?=	MmTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
NCI-H460	NETUUolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NYjmZpFCUUN3ME2wMlAyQTd2IN88US=>	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
A204	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEHafZVKSzVyPUCuNFIzPDlizszN	Mle3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
CHL-1	NUHVflZET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHu3Z3VKSzVyPUCuNFIzPTFizszN	NGTPXXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
DU-4475	NX3CT29NT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MlzPTWM2OD1yLkCyNlYyKM7:TR?=	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
CGTH-W-1	M2CxPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MVjJR\|UxRTBwMEKzOFkh\|ryP	M4DQXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
HCC2218	M4fWR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MlzxTWM2OD1yLkCyOFk1KM7:TR?=	M3XiclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
A2780	M3TMTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYDrV\|lXUUN3ME2wMlAzPTNzIN88US=>	MnTnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
NCI-H720	NHy0NJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MoDpTWM2OD1yLkCyOVQ3KM7:TR?=	NHfj[YY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
NCI-N87	Mo\CS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NXPYZo1mUUN3ME2wMlAzPTl\|IN88US=>	MoHBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
CHP-212	MnvIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NUXv[ZlQUUN3ME2wMlAzPjJ6IN88US=>	NYDpOlJqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
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LB2241-RCC	NXnSNIJFT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M1vydWlEPTB;MD6xNFA2PCEQvF2=	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
SN12C	MorRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M{nJb2lEPTB;MD6xNFExPyEQvF2=	NF\4T209[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
A498	MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWfJR\|UxRTBwMUCxPEDPxE1?	MkXxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
PANC-03-27	NWT6eY9lT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Mnz2TWM2OD1yLkGwNlg3KM7:TR?=	NYTSVXJ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
NCI-H1581	MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXzJR\|UxRTBwMUCyPUDPxE1?	NGfyVFQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
U-87-MG	M2[5Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M{HOXmlEPTB;MD6xNFMxPyEQvF2=	NVzse3RORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
G-401	M2\Pd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mn[zTWM2OD1yLkGwN\|U{KM7:TR?=	MoC4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
SiHa	M{HLTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M2D6d2lEPTB;MD6xNFQ5PiEQvF2=	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
U251	NVnwOGluT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M3rCdGlEPTB;MD6xNFUzPSEQvF2=	NHzCXI49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
MMAC-SF	NYXUepZRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NH3XOFdKSzVyPUCuNVA2OzFizszN	MmfkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
BB65-RCC	NVWxUXJ7T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MWjJR\|UxRTBwMUC1OVQh\|ryP	NGP3O489[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
NKM-1	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmPCTWM2OD1yLkGwOlY3KM7:TR?=	M1HmVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
HD-MY-Z	NFfIZ4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3\QVGlEPTB;MD6xNFg4OyEQvF2=	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
TGBC11TKB	NETHSYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MmjJTWM2OD1yLkGwPVA{KM7:TR?=	M4S4XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
COLO-679	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4XUbGlEPTB;MD6xNVIyOSEQvF2=	MmXsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
TE-8	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXnJR\|UxRTBwMUGyOlQh\|ryP	MnXPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
SK-MEL-28	NGK1RZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MkTqTWM2OD1yLkGxN\|IyKM7:TR?=	NI\aSFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
SH-4	NYf0Z4tXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NGfPXXFKSzVyPUCuNVE{PTZizszN	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
KALS-1	NF73bGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NGLtfWxKSzVyPUCuNVE{QTNizszN	M{TrVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
RKO	NFmzSmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NVfpfFBqUUN3ME2wMlEyPzZ2IN88US=>	NXnac29SRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
OMC-1	M{fURmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M{m5bmlEPTB;MD6xNVc6PyEQvF2=	NWnQ[ohoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
BT-549	NGP3NYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MoLHTWM2OD1yLkGxO\|k6KM7:TR?=	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
NCI-H28	MkX3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M2\iUmlEPTB;MD6xNVgyPyEQvF2=	M{nzTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
RXF393	NULBXot2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M4e0V2lEPTB;MD6xNVk5OiEQvF2=	MkfZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
COLO-829	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M3myWWlEPTB;MD6xNlAxPSEQvF2=	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
HMV-II	NFHFfVBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MoHFTWM2OD1yLkGyNFIh\|ryP	M2H5NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
SW1990	NYjxTZQzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NXXkfHhlUUN3ME2wMlEzOTV5IN88US=>	NX3lOIFxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
NCI-H1437	NGnScWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MUDJR\|UxRTBwMUKxOVch\|ryP	M1\hNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
SNB75	MnywS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3;zeGlEPTB;MD6xNlE3QSEQvF2=	Mn\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
EW-3	NYq4RXN7T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M{L2[WlEPTB;MD6xNlY3OSEQvF2=	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
SAS	MlLuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHvQbVJKSzVyPUCuNVI3QSEQvF2=	MonlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
NCI-H1666	MmLlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVywXIlPUUN3ME2wMlEzPzd2IN88US=>	NWDQT\|lsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
A375	MkPPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NG\PWFVKSzVyPUCuNVI4PzhizszN	M4C5SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
CAMA-1	M4LlUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NH3VUndKSzVyPUCuNVI6OjRizszN	NELVUow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
HuP-T4	MlPjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MkfzTWM2OD1yLkGyPVQ{KM7:TR?=	NVz5O3lmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
NCI-H292	M2fXOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NX3HSXVtUUN3ME2wMlE{OTFizszN	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
PC-14	MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEDYUVlKSzVyPUCuNVMzOTFizszN	M1nxW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
BPH-1	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MoLWTWM2OD1yLkGzOFEh\|ryP	NWPWdG1ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
GAK	NYnDenlXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NXzrWVQ1UUN3ME2wMlE{PTZ5IN88US=>	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
VMRC-RCZ	NEj6WnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVPJR\|UxRTBwMUO3OlIh\|ryP	Ml\PQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
SK-MEL-24	M3iwRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MnfZTWM2OD1yLkGzPFch\|ryP	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
LB831-BLC	NVKzV2hYT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2DnSGlEPTB;MD6xN\|g5OyEQvF2=	NVTYfJoxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
NCI-H2452	M1HiO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYHSSW5zUUN3ME2wMlE{QTV4IN88US=>	MkLpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
RT-112	MoDXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MontTWM2OD1yLkG0NFM2KM7:TR?=	NEntPYs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
GP5d	Ml\rS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M4[1VGlEPTB;MD6xOFExPCEQvF2=	MnzDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
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MPP-89	MmewS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHjF[JlKSzVyPUCuNVQyOjVizszN	Mm\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
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NCI-H630	M4q0XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmjITWM2OD1zNj64OVM4KM7:TR?=	M13BWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
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NCI-H1693	M17vNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NEHEUXNKSzVyPUKwMlY2ODVizszN	NIPGWIs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
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SNU-449	M2Lzbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYL5VIQzUUN3ME2yOE45PzRzIN88US=>	NU\vR3FGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
KE-37	MljnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHjIO3RKSzVyPUK1MlA6OjhizszN	M{fsflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
NCI-H1623	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NFrYZmRKSzVyPUK3MlE{ODNizszN	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
MOLT-13	M{fKVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MX\JR\|UxRTJ5LkO1NVYh\|ryP	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
COLO-741	M2DxfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWHJR\|UxRTNzLkOzN\|Mh\|ryP	M2PFeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
NB6	MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NF7hNFZKSzVyPUOyMlI5PzZizszN	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNFk1QDBxJ{7TRW5ITVJ:L3G+
MOLT-16	MoXQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NYPtPJV4UUN3ME2zN{4xODV3IN88US=>	NIKweGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
IST-MES1	M1HsU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MXnJR\|UxRTN2LkC1OFEh\|ryP	NEjx[449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
A4-Fuk	MneyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Mln4TWM2OD1\|NT6wOlYh\|ryP	M1voS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
CAL-85-1	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M3PW[2lEPTB;M{WuNlgyPyEQvF2=	MojPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
CCRF-CEM	M{jORmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Ml7hTWM2OD1\|Nz6xOVM3KM7:TR?=	NFfNeW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
HAL-01	NV;kRYxvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NUXYZ3JzUUN3ME2zPE41QDh7IN88US=>	Mne2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
HEL	NUfxRXZqT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MVvJR\|UxRTR\|LkO0NFkh\|ryP	NGLqWIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
EW-1	MlXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MWjJR\|UxRTR\|LkS3Olgh\|ryP	MoLiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVA6PDhyLze+V2FPT0WUPD;hQi=>
MDA-MB-231	M1jFUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MXLJR\|UxRTR3LkGxPVkh\|ryP	M17IZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFExQTR6MD:nQnNCVkeHUkyvZV4>
ABC-1	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NX6zNIl6UUN3ME20Ok45PTN6IN88US=>	NY\v[JVbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
NCI-H446	NXH4UmlrT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M{m5SGlEPTB;NEiuOVA2QCEQvF2=	NFnTOGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
MHH-PREB-1	MnnPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MluzTWM2OD12OT62NFA4KM7:TR?=	NHnEb5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyODl2OECvK\|5USU6JRWK8M4E,
DOHH-2	NFnBWYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M3\icmlEPTB;NEmuOlY2PiEQvF2=	NXzkbodjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?
GCT	MkT5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M17QSGlEPTB;NEmuOlk4PiEQvF2=	NXzNeYRmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTB7NEiwM{c,W0GQR1XSQE9iRg>?

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	α-Synuclein / HSP90 / HSP70 / HSP32 / αB-Crystallin; PubMed: 20090920 PLoS One Immunoblot analysis of heat shock protein induction. Cells were treated with 17-AAG (1–200 nM, 24 h), or subjected to heat shock (HS: 44°C, 30 min, 24 h recovery) or to MG-132 (MG: 1 µM, 24 h). Cell lysates were prepared and immunoblot analysis was carried out with antibodies against the individual proteins as indicated on the right. Co, untreated control. EGFR / PDGFR / AKT / MAPK / c-RAS; PubMed: 18682579 Neuro Oncol 17-AAG promotes the degradation of HSP90 client proteins that are involved in the pathology of glioblastoma multiforme (GBM). Glioma cell lines were treated with increasing concentrations of 17-AAG for 48 h, and protein lysates were run on Western blots. HSP90 client proteins known to be involved in gliomagenesis showed degradation while nonclient proteins remained unaffected by 17-AAG treatment. pAKT / pMAPK / pRb; PubMed: 18682579 Neuro Oncol The phosphorylated states of signaling proteins involved in glioma growth are also affected by 17-AAG treatment. Cleaved PARP / Survivin; PubMed: 18827603 J Thorac Oncol Western analysis of expression levels of cleaved PARP and Survivin in MM cell lines. β-Actin served as loading control. LATS1 / p-LATS1 / YAP / CTGF; PubMed: 25712415 J Cell Mol Med Effects of 17-AAG on the expression of LATS1, p-LATS1, YAP and CTGF. The expression levels of YAP and CTGF proteins, indicated by Western blot assay, were down-regulated, while LATS1 was upregulated in a dose-dependent manner in 17-AAG treated groups compared to the untreated group.	20090920 18682579 18827603 25712415
Immunofluorescence	STAT3; PubMed: 27279418 Mol Med Rep Effects of 17-AAG on STAT3 expression of H446 cells. (A) Control group; (B) 3.125 mg/l; (C) 6.25 mg/l. 17-AAG, 17-allylamino-17-demethoxygel-danamycin; STAT3, signal transducer and activator of transcription 3; DAPI, 4′,6-diamidino-2-phenylindole. YAP / TAZ; PubMed: 25290150 PLoS One Reduction in cytoplasmic distribution accompanied by increased nuclear localization of YAP/TAZ was more apparent in cells treated with 45 nM 17-AAG (red channel for YAP/TAZ and blue channel for nucleus). Statistical comparisons were performed using one-way Kruskal-Wallis multiple comparison, ***p<0.001 compared with control (0 nM). β-catenin / E-cadherin; PubMed: 25290150 PLoS One Co-localization of β-catenin (Red) and E-cadherin (Green) was observed at the cell-cell junctions (white arrows) both in siCtrl and siCTGF cells after 17-AAG treatment. Scale bar is 20 µm.	27279418 25290150
Growth inhibition assay	Cell viability; PubMed: 18682579 Neuro Oncol 17-AAG inhibits the growth of human glioma cell lines. Human glioma cell lines and a nontumorigenic fibroblast cell line were treated with increasing concentrations of 17-AAG for 4 days, and the effect on cell growth was assessed by a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay. The 50% inhibitory concentration (IC50) of all glioma cell lines ranged from 0.05 to 0.5 μM 17-AAG, as compared to 1.5 μM for the nontumorigenic cells.	18682579

体内研究

17-AAG作用于携带3T3-src, B16或CT26移植瘤裸鼠，与Hsp90结合具有高亲和力，IC50为8-35 nM，而作用于正常组织时，IC50为200-600 nM。^[1] 17-AAG按50 mg/kg左右剂量处理，引起AR, HER2, HER3, 和 Akt表达明显降低，降低达50%以上，这种作用存在剂量依赖性，结果导致雄激素依赖型 (CWR22)和非依赖型(CWR22R 和CWRSA6) 前列腺移植瘤生长受抑制，抑制率分别为67%, 80%和 68%。^[2]

激酶实验：^[1]	- 合并 Hsp90结合实验: 纯化的Hsp90蛋白或过量表达HER-2的癌细胞(BT474, N87, SKOV3 和 SKBR3) 裂解物或BT474乳腺癌细胞溶于溶解buffer(20 mM HEPES, pH 7.3, 1 mM EDTA, 5 mM MgCl₂, 100 mM KCl) ，与不同浓度17-AAG在4^oC下温育30分钟，然后和生物素-GM链接的BioMag链霉亲和素磁珠在4^oC下温育1小时。试管放置于磁架上,除去未结合的上清液。在溶解buffer中清洗三次磁珠，然后在SDS–PAGE 样本缓冲液中在95^oC下加热5分钟。在SDS蛋白凝胶上进行样本分析,使用特点抗体进行Western Blotting。使用Bio-rad Fluor-S 多重图像测量Western Blot条带，计算抑制Hsp90与生物素-GM结合的百分数。测定IC50。
细胞实验：^[1]	- 合并 Cell lines: BT474, SKBR3, N87, SKOV3, MCF7, MDA468, Hs578T, Hs578Bst, A549, HT29, U87, SKMG3, HT1080, RPTEC, NDF, HMVEC, HMEC, HUVEC, 和 PBMC Concentrations: 溶于DMSO, 终浓度为10 μM 左右 Incubation Time: 5天 Method: 细胞按每孔2000个接种在96孔板上，终培养体积为100 μL，培养24小时，然后加入浓度不断增高的17-AAG，温育5天。使用 Celltiter 96 AQueous 非放射性细胞增殖检测试剂盒测定存活细胞数。测定IC50。 (Only for Reference)
动物实验：^[2]	- 合并 Animal Models: 皮下注射雄激素依赖性CWR22移植瘤的雄性 nu/nu 无胸腺小鼠，皮下注射雄激素非依赖性的CWR22R和CWRSA6移植瘤的雌性nu/nu 无胸腺小鼠。 Dosages: 50 mg/kg 左右 Administration: 腹膜注射 (Only for Reference)

参考文献

溶解度 (25°C)

体外	DMSO	100 mg/mL (170.73 mM)
	Water	Insoluble
	Ethanol	''5 mg/mL
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 5%DMSO+corn oil	10mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Tanespimycin (17-AAG) SDF

分子量	585.69
化学式	C₃₁H₄₃N₃O₈
CAS号	75747-14-7
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	CP127374, NSC-330507, KOS 953

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

×

在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。.

连续稀释计算器方程

连续稀释
初始浓度:
稀释倍数:

计算结果

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

分子量计算器

通过输入化合物的化学式来计算其分子量：

注：化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量	浓度	体积	分子量
=	×	×
计算

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT00103272	Terminated	Drug: tanespimycin\|Drug: bortezomib	Adult Acute Basophilic Leukemia\|Adult Acute Eosinophilic Leukemia\|Adult Acute Megakaryoblastic Leukemia (M7)\|Adult Acute Minimally Differentiated Myeloid Leukemia (M0)\|Adult Acute Monoblastic Leukemia (M5a)\|Adult Acute Monocytic Leukemia (M5b)\|Adult Acute Myeloblastic Leukemia With Maturation (M2)\|Adult Acute Myeloblastic Leukemia Without Maturation (M1)\|Adult Acute Myeloid Leukemia With 11q23 (MLL) Abnormalities\|Adult Acute Myeloid Leukemia With Inv(16)(p13;q22)\|Adult Acute Myeloid Leukemia With t(16;16)(p13;q22)\|Adult Acute Myeloid Leukemia With t(8;21)(q22;q22)\|Adult Acute Myelomonocytic Leukemia (M4)\|Adult Erythroleukemia (M6a)\|Adult Pure Erythroid Leukemia (M6b)\|Anaplastic Large Cell Lymphoma\|Angioimmunoblastic T-cell Lymphoma\|Extranodal Marginal Zone B-cell Lymphoma of Mucosa-associated Lymphoid Tissue\|Nodal Marginal Zone B-cell Lymphoma\|Recurrent Adult Acute Lymphoblastic Leukemia\|Recurrent Adult Acute Myeloid Leukemia\|Recurrent Adult Diffuse Large Cell Lymphoma\|Recurrent Adult T-cell Leukemia/Lymphoma\|Recurrent Cutaneous T-cell Non-Hodgkin Lymphoma\|Recurrent Grade 1 Follicular Lymphoma\|Recurrent Grade 2 Follicular Lymphoma\|Recurrent Grade 3 Follicular Lymphoma\|Recurrent Mantle Cell Lymphoma\|Recurrent Marginal Zone Lymphoma\|Recurrent Mycosis Fungoides/Sezary Syndrome\|Recurrent Small Lymphocytic Lymphoma\|Refractory Chronic Lymphocytic Leukemia\|Small Intestine Lymphoma\|Splenic Marginal Zone Lymphoma\|Waldenström Macroglobulinemia	National Cancer Institute (NCI)	April 2005	Phase 1
NCT00098488	Terminated	Drug: tanespimycin\|Biological: rituximab	B-cell Chronic Lymphocytic Leukemia\|Prolymphocytic Leukemia\|Refractory Chronic Lymphocytic Leukemia	National Cancer Institute (NCI)	April 2005	Phase 1
NCT00118248	Completed	Drug: tanespimycin	Recurrent Thyroid Cancer\|Stage IV Follicular Thyroid Cancer\|Stage IV Papillary Thyroid Cancer\|Thyroid Gland Medullary Carcinoma	National Cancer Institute (NCI)	December 2004	Phase 2
NCT00098423	Completed	Drug: tanespimycin\|Drug: cytarabine	Accelerated Phase Chronic Myelogenous Leukemia\|Adult Acute Basophilic Leukemia\|Adult Acute Eosinophilic Leukemia\|Adult Acute Megakaryoblastic Leukemia (M7)\|Adult Acute Minimally Differentiated Myeloid Leukemia (M0)\|Adult Acute Monoblastic Leukemia (M5a)\|Adult Acute Monocytic Leukemia (M5b)\|Adult Acute Myeloblastic Leukemia With Maturation (M2)\|Adult Acute Myeloblastic Leukemia Without Maturation (M1)\|Adult Acute Myeloid Leukemia With 11q23 (MLL) Abnormalities\|Adult Acute Myeloid Leukemia With Inv(16)(p13;q22)\|Adult Acute Myeloid Leukemia With t(16;16)(p13;q22)\|Adult Acute Myeloid Leukemia With t(8;21)(q22;q22)\|Adult Acute Myelomonocytic Leukemia (M4)\|Adult Erythroleukemia (M6a)\|Adult Pure Erythroid Leukemia (M6b)\|Blastic Phase Chronic Myelogenous Leukemia\|Chronic Myelomonocytic Leukemia\|de Novo Myelodysplastic Syndromes\|Previously Treated Myelodysplastic Syndromes\|Recurrent Adult Acute Lymphoblastic Leukemia\|Recurrent Adult Acute Myeloid Leukemia\|Refractory Anemia With Excess Blasts in Transformation\|Relapsing Chronic Myelogenous Leukemia\|Secondary Acute Myeloid Leukemia\|Secondary Myelodysplastic Syndromes	National Cancer Institute (NCI)	November 2004	Phase 1

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操作手册

如果有其他问题，请给我们留言。

常见问题及建议解决方法

问题 1：
What kind of subtypes does this compound inhibit? HSP90α or HSP90β ?
回答：
17-AAG can inhibit both HSP90α and HSP90β, but it is unclear how much are they affected respectively. You should note that Hsp90 derived from tumor cells has a 100-fold higher binding affinity for 17-AAG than that from normal cells according to this paper. http://www.nature.com.ezp-prod1.hul.harvard.edu/nature/journal/v425/n6956/full/nature01913.html

HSP (HSP90) Signaling Pathway Map

HSP (HSP90) Inhibitors with Unique Features

选择性强的HSP90抑制剂

NVP-BEP800 : HSP90β-selective, IC50=58 nM.
活性最高的HSP90抑制剂

KW-2478 : HSP90, IC50=3.8 nM.
用于临床的HSP90抑制剂

17-DMAG (Alvespimycin) HCl : Phase II for Kidney Cancer.
最新的HSP90抑制剂

XL888 : ATP-competitive inhibitor of HSP90 with IC50 of 24 nM.

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Tanespimycin (17-AAG)

客户使用Selleck生产的Tanespimycin (17-AAG)发表文献84篇：

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HSP (HSP90)抑制剂选择性比较

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推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

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化学数据 Download Tanespimycin (17-AAG) SDF

动物体内配方计算器 (澄清溶液)

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摩尔浓度计算器

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

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稀释计算器

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

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连续稀释

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分子量计算器

总分子量：g/mol

摩尔浓度计算器

临床试验信息 (data from https://clinicaltrials.gov, updated on 2021-01-06)

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HSP (HSP90) Signaling Pathway Map

HSP (HSP90) Inhibitors with Unique Features

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