Celastrol (NSC 70931)

For research use only. Not for use in humans.

目录号:S1290 别名: Tripterine 中文名称:雷公藤红素

Celastrol (NSC 70931) Chemical Structure

CAS No. 34157-83-0

Celastrol (NSC 70931, Tripterine)是一种有效的蛋白酶抑制剂,有效且优先抑制胰凝乳蛋白酶样的纯化的20S proteasome 的活性,IC50为2.5 μM。Celastrol 可通过ROS/JNK信号传导途径诱导凋亡和自噬。Celastrol 通过激活线粒体凋亡可抑制帕金森氏病的多巴胺能神经元死亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1040.77 现货
RMB 898.79 现货
RMB 2449.18 现货
RMB 3836.42 现货
有超大折扣

今日订购,明日送达,全国免运费!

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Celastrol (NSC 70931)发表文献8篇:

客户使用该产品的2个实验数据:

  • SK-BR-3, A549, HCT-116 and BT-474 cells were incubated with or without X66 for 1 h before exposed to GM, celastrol or MG132 for 8 h. Cell lysates were analyzed by Western blot with indicated antibodies.

    Oncotarget, 2016, 7(20):29648-63.. Celastrol (NSC 70931) purchased from Selleck.

    Effects of compound 10 and celastrol on client proteins degradation. Both compound 10 and celastrol induces decreases of Hsp90 client proteins (Akt and Cdk4) through a concentration -dependent manner by Western blot. Meanwhile, compound 10 and celastrol have no effect on the expression level of Hsp90.

    Eur J Med Chem, 2017, 136:63-73. Celastrol (NSC 70931) purchased from Selleck.

产品安全说明书

Proteasome抑制剂选择性比较

生物活性

产品描述 Celastrol (NSC 70931, Tripterine)是一种有效的蛋白酶抑制剂,有效且优先抑制胰凝乳蛋白酶样的纯化的20S proteasome 的活性,IC50为2.5 μM。Celastrol 可通过ROS/JNK信号传导途径诱导凋亡和自噬。Celastrol 通过激活线粒体凋亡可抑制帕金森氏病的多巴胺能神经元死亡。
特性 Celastrol是一种强效的抗氧化剂和抗炎药。
靶点
20S proteasome [1]
(Cell-free assay)
2.5 μM
体外研究

Celastrol在5 μM时,抑制胰凝乳蛋白酶样,PGPH样,胰蛋白酶样的纯化的20S蛋白酶体活性,分别抑制 80%,5%,和<1%。而在10 μM时,抑制这三种蛋白酶体活性分别为~90%,15%,和<1%。Celastrol 作用于PC-3细胞,显著抑制蛋白酶体糜蛋白酶活性,这种作用具有浓度依赖性。2.5 μM到 5 μM Celastrol作用于PC-3细胞,诱导caspase-3 活性提高4.7倍到5.5倍。Celastrol(5 μM) 处理细胞1小时后,蛋白酶体靶蛋白 IκB-α 和 Bax水平提高。Celastrol(2.5 μM)处理LNCaP细胞,抑制40%蛋白酶体,胰凝乳蛋白酶样活性降低,泛素化蛋白积累增加。Celastrol (2.5 μM)作用于Celastrol处理的LNCaP细胞,诱导细胞凋亡,caspase-3活性水平提高(高达3.5倍),且促进PARP裂解和细胞凋亡形态发生。[1]Celastrol (300 nM)抑制LPS诱导的人体单核细胞和巨噬细胞中TNF-α和IL-1β的生产。Celastrol (100 nM)也降低LPS诱导的小胶质细胞中的Ⅱ类MHC分子。Celastrol作用于巨噬细胞,强抑制LPS和IFN-γ诱导的NO产生,IC50为200 nM。Celastrol作用于血管内皮细胞,强抑制TNF-α和IFN-γ诱导的NO产生,IC50为200 nM。[2] Celastrol (2.5 μM) 作用于KBM-5细胞,加强TNF和化疗药物诱导的细胞凋亡,且抑制入侵,这两者同时由NF-κB的激活调节。Celastrol (2.5 μM) 作用于KBM-5细胞,抑制 TNF诱导的参与抗凋亡(IAP1, IAP2, Bcl-2, Bcl-XL, c-FLIP, 和 survivin),增殖(cyclin D1 和 COX-2),入侵(MMP-9),和血管生成(VEGF)的基因产物的表达。Celastrol (5 μM)抑制TNF诱导的 IkappaBα激酶的激活,IkappaBα的磷酸化,IkappaBα的降解,p65核易位和磷酸化,及NF-κB调节的报告基因的表达。[3] Celastrol 抑制RPMI 8226, KATOIII, UM-SCC1, U251MG 和 MDA-MB-231 细胞增殖,IC50分别为0.52 μM, 0.54 μM, 0.76 μM, 0.69 μM 和 0.67 μM。Celastrol(1 μM) 抑制RPMI 8226的生长,降低cyclin D1和cyclin E的水平,但增加p21和p27的水平。Celastrol 作用于RPMI-8226细胞,通过caspase-8激活, Bid 裂解, caspase-9 激活, caspase-3 激活, PARP 裂解,及通过下调抗凋亡蛋白,而诱导细胞凋亡。Celastrol (1 μM) 作用于RPMI-8226 细胞,抑制Akt通路,激活JNK激酶。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
RAW264.7 MkL2RY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? M3P4OVI1KGi{cx?= M1TUV2FvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCUQWeyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJGxRWy2rbnT1Z4VlKE6RIIDyc4R2[3Srb36geJJm[XSnZDCzJIhzeyCkZX\vdoUhVFCVIHPoZYxt\W6pZTDhd5Nme3OnZDDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KHKnYXflcpQh[XO|YYmsJGlEPTBiPTCwMlI{KM7:TT6= M2[xZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOEC5NFc3Lz5zMUiwPVA4PjxxYU6=
RAW264.7 NFrvbnpCdnSraX7mcIFudWG2b4L5JIF{e2G7 M2iwNVI1KGi{cx?= Ml72RY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKFKDV{K2OE44KGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gUHBUNWmwZIXj[YQhVkZva3HwdIFDKGGldHn2ZZRqd25idILlZZRm\CB|IHjyd{Bj\W[xcnWgUHBUKGOqYXzs[Y5o\SCjc4Pld5Nm\CCjZoTldkAzPCCqcoOgZpkhW0WDUDDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyIE2gNE4zPyEQvF2u NHPmcIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUiwPVA4Pid-MUG4NFkxPzZ:L3G+
RPMI8226 MnTiS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MV\Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDSVG1KQDJ{NjDj[YxteyxiSVO1NEA:KDNizszNMi=> M{T4NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MU[0NVk4Lz5zOEG2OFE6PzxxYU6=
HeLa M1zvVmZ2dmO2aX;uJIF{e2G7 NGfP[pRKdmirYnn0bY9vKG:oIGTOSk1idHCqYT3pcoR2[2WmIF7GMYtieHCjQjDhZ5RqfmG2aX;uJIlvKGi3bXHuJGhmVGFiY3XscJMh[nliU1XBVEBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwJF0hOC5zNTFOwG0v NWnCSmZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4OFE6ODZpPkG4PFQyQTB4PD;hQi=>
RAW264.7 NVXrOnA4TnWwY4Tpc44h[XO|YYm= MYDJcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIF7GMYtieHCjQjDhZ5RqfmG2aX;uJIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHL5JHNGSVBicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OCB;IECuN{DPxE1w NW\2VnlmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi4OFE6ODZpPkG4PFQyQTB4PD;hQi=>
SH-SY5Y NHLlNXdHfW6ldHnvckBie3OjeR?= MkLzUoV2em:ycn;0[YN1cW:wIHHnZYlve3RiYnX0ZU1idXmub3nkJJBmeHSrZHWgNU01Oi2rbnT1Z4VlKHSxeHnjbZR6KGmwIHj1cYFvKFOKLWPZOXkh[2WubIOgZZN{\XO|ZXSgZZMhdGGldHH0[UBl\Wi7ZILv[4Vv[XOnIILlcIVie2VuIFXDOVAhRSByLkCzO|Y1KM7:TT6= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTF|OEi1PUc,OTlzM{i4OVk9N2F-
HeLa MmXsSpVv[3Srb36gZZN{[Xl? M{nPN|IhcHK| NUfoOZI2SW2ybHnmbYNifGmxbjDv[kBJW0ZzIITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCrbjDoeY1idiCqZXH0JJNpd2OtLXnu[JVk\WRiSHXMZUBk\WyuczDhd5Nme3OnZDDhd{BoemGwdXzlJIZwem2jdHnvckB1emWjdHXkJFEhcHJiYnXmc5JmKGinYYSgd4hw[2tiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDJiaILzJJdqfGixdYSgdoVkd3[ncomgeIlu\SCkeTDpcY12dm:leYTvZ4hmdWmlYXygd5RicW6rbnesJGVEPTBiPTCxMlEh|ryPLh?= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTVyMkC1O{c,OTl3MEKwOVc9N2F-
SK-N-SH MmHmR5l1d3SxeHnjbZR6KGG|c3H5 MUK3NkBpenN? NInEbHlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUUy2QLWPIJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGOgZZN{[XluIFPDOVAhRSBzLk[g{txONg>? NV7ubI56RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm1NFIxPTdpPkG5OVAzODV5PD;hQi=>
HeLa MkX2SpVv[3Srb36gZZN{[Xl? M3rFZ|MhcHK| NEPYS5VCdXCuaX\pZ4F1cW:wIH;mJGhUTjFidILhcpNkemmydHnvcoFtKGGldHn2bZR6KGmwIHj1cYFvKEinTHGgZ4VtdHNiYYPz[ZN{\WRiYYOg[5JidnWuZTDmc5Ju[XSrb36gdJJmfHKnYYTl[EBnd3JiMzDodpMh[nliaX3teY5w[3m2b3Po[Y1q[2GuIIP0ZYlvcW6pLDDFR|UxKD1iMj62JO69VS5? M1HH[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NUCyNFU4Lz5zOUWwNlA2PzxxYU6=
HeLa MoXlR5l1d3SxeHnjbZR6KGG|c3H5 NXPlNG9zPzJiaILz NH\H[VhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{KGGodHXyJFczKGi{czDifUBOXFNiYYPzZZktKEOFNUCgQUA{KM7:TT6= MkfEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl3MEKwOVcoRjF7NUCyNFU4RC:jPh?=
HeLa MVnGeY5kfGmxbjDhd5NigQ>? M3;PbGlvcGmkaYTpc44hd2ZiTl[tb4FxeGGEIHHjeIl3cXS7IHnuJIh2dWGwIFjlUIEh[2WubIOgZpkhW0WDUDDy[ZBwenSncjDhd5NigSxiSVO1NEA:KDBwMUWg{txONg>? NYKzT3A4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0Olk5QDdpPkKwOFY6QDh5PD;hQi=>
LNCAP M{Wx[mFvfGm2dX3vdkBie3OjeR?= NUjNPHlESW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5QhcHWvYX6gUG5ESVBiY3XscJMtKEmFNUCgQUAzNjVizszNMi=> MkD0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4Mke1OVYoRjJyNkK3OVU3RC:jPh?=
PC12 MXzDfZRweHKxdHXjeIl3\SCjc4PhfS=> M33P[mN6fG:ycn;0[YN1cX[nIHHjeIl3cXS7IHHnZYlve3RidD3CVGgucW6mdXPl[EBk\WyuIHThcYFo\SCrbjDyZZQhWENzMjDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKH[rYXLpcIl1gSxiSVO1NEA:KDNwMUWg{txONg>? Mlr4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB4Mke1OVYoRjJyNkK3OVU3RC:jPh?=
PC12 M{DyfWN6fG:ycn;0[YN1cX[nIHHzd4F6 NVm2eXR6OS54IIXN NGn6ZohEgXSxcILveIVkfGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IIStRnBJNWmwZIXj[YQh[2WubDDkZY1i\2ViaX6gdoF1KFCFMUKgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC4aXHibYxqfHliYYSgNU43KHWP NUPzbWRQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC2Nlc2PTZpPkKwOlI4PTV4PD;hQi=>
THP1 NUnCWGIzSXCxcITvd4l{KGG|c3H5 MVWyOEBpenN? NHrVUItKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJHRTSUmOLYLld4l{fGGwdDDoeY1idiCWSGCxJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCjbn7lfIlvNVZic4ThbY5qdmduIFXDOVAhRSBzNTFOwG0v NVTWVolTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4OlQ{PDJpPkKwPFY1OzR{PD;hQi=>
293T MkHFSpVv[3Srb36gZZN{[Xl? MVuzNEBucW6| M{TYTmlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUmYuc2GycHHCJIFkfGm4YYTpc44hcW5iaIXtZY4hOjl|VDDj[YxteyCrbnP1ZoF1\WRiZn;yJFMxKG2rboOg[o9tdG:5ZXSgZpkhfHKnYYTl[EB4cXSqIFzQV{Bnd3JiNjDodpMh[nliZIXhcE1tfWOrZnXyZZNmKHKncH;yeIVzKGG|c3H5MEBKSzVyIE2gNE4yPyEQvF2u NHrHPVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUO5N|AxPCd-MkGzPVMxODR:L3G+
293T NEi4e5FEgXSxdH;4bYNqfHliYYPzZZk> NHnmTolEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckAzQTOWIHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwhfmmjYnnsbZR6KGK7IHT1ZYwudHWlaX\ldoF{\SC{ZYDvdpRmeiCjc4PhfUwhUUN3MDC9JFAvPjdizszNMi=> M3vlOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzM{mzNFA1Lz5{MUO5N|AxPDxxYU6=
RAW264 MmDSSpVv[3Srb36gZZN{[Xl? NGW3VGEzPCCqcoO= NFnGdmNKdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJG5QKHC{b3T1Z5Rqd25iaX6gcY92e2ViUlHXNlY1KGOnbHzzJIFnfGW{IEK0JIhzew>? MlPUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF|OUOwNFQoRjJzM{mzNFA1RC:jPh?=
NCI-H460 NWnOVGJ1S3m2b4TvfIlkcXS7IHHzd4F6 NGXleY9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBPS0lvSES2NEBk\WyuczDh[pRmeiCxdnXycolocHRiaX7jeYJifGmxbjDifUBOXFRiYYPzZZktKEmFNUCgQUAyOi5|IN88UU4> NWXDT4pFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5OFI4PjVpPkKxPVQzPzZ3PD;hQi=>
RAW264.7 NVfsWG55SW62aXnu[oxidW2jdH;yfUBie3OjeR?= MViyOEBpenN? MmrURY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44h[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDy[YFo\W62IH3leIhw\CxiSVO1NEA:KDFizszNMi=> MoTBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7N{i2O|YoRjJzOUe4Olc3RC:jPh?=
RAW264.7 MnPIRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NUG5XWNXOjRiaILz MlLHRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIH7peJJq[yCxeHnk[UBxem:mdXP0bY9vKGGodHXyJFI1KGi{czDifUBoemmnc4OgZZN{[XluIFnDOVAhRSBzIN88UU4> NUT6VWppRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwNlQxOzNpPkKyNFI1ODN|PD;hQi=>
RAW264.7 MWjGeY5kfGmxbjDhd5NigQ>? M2KwXFE5KGi{cx?= NXvBTW9EUW6qaXLpeIlwdiCxZjDMVHMue3SrbYXsZZRm\CCQRnvhdJBiSiCjY4TpeoF1cW:wIITyZY5{\mWldHXkJIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHmeIVzKDF6IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTBiPTCwMlIh|ryPLh?= M3PqelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{N{C1NFIxLz5{MkewOVAzODxxYU6=
spleen adherent cells MXnBcpRq[XK2aILpeIlkKGG|c3H5 M3rXPFIxOCC3Zx?= MYfBcpRq[XK2aILpeIlkKGWoZnXjeEBqdiCOZYfpd{Bz[XRiYXTqeZZidnRvaX7keYNm\CCjcoTodol1cXNibX;k[Ywhe3CuZXXuJIFlcGW{ZX70JINmdGy|IILld5RqdXWuYYTl[EB4cXSqIIPvcolk[XSnZDDNeIIheG:|dDDpd49t[XSrb36gZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFPDVlIh\XiycnXzd4lwdiCjdDCyNFAhfWduIHnwJJN1[XK2aX7nJIZzd21iYYL0bJJqfGm|IH;ud4V1KGGwZDDjc451cW63ZXSgeY5qdnSncoL1dJRm\CC3boTpcC=> Mn7tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6NUSxPVMoRjJ{OEW0NVk{RC:jPh?=
spleen adherent cells MXXBcpRq[XK2aILpeIlkKGG|c3H5 NUnheFdrOjByIIXn MnTWRY51cWG{dHjybZRq[yCnZn\lZ5QhcW5iTHX3bZMhemG2IHHkbpV3[W62LXnu[JVk\WRiYYL0bJJqfGm|IH3v[IVtKHOybHXlckBi\GincnXueEBk\WyuczDy[ZN1cW23bHH0[YQhf2m2aDDzc45q[2G2ZXSgUZRjKHCxc4SgbZNwdGG2aX;uJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDHVm8wU0NicILv[JVkfGmxbjDheEAzODBidXesJIlxKHO2YYL0bY5oKG[{b32gZZJ1cHKrdHnzJI9ve2W2IHHu[EBkd262aX71[YQhfW6rboTldpJ2eHSnZDD1cpRq NVO5blA{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4OVQyQTNpPkKyPFU1OTl|PD;hQi=>
spleen adherent cells M2Tqb2FvfGmjcoTodol1cWNiYYPzZZk> MYWyNFAhfWd? NH3s[XpCdnSrYYL0bJJqfGmlIHXm[oVkfCCrbjDM[ZdqeyC{YYSgZYRrfX[jboStbY5lfWOnZDDhdpRpemm2aYOgcY9l\Wxic4Ds[YVvKGGmaHXy[Y51KGOnbHzzJJJme3SrbYXsZZRm\CC5aYToJJNwdmmlYYTl[EBOfGJicH;zeEBqe2:uYYTpc44h[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJG1EWDFicILv[JVkfGmxbjDheEAzODBidXesJIlxKHO2YYL0bY5oKG[{b32gZZJ1cHKrdHnzJI9ve2W2IHHu[EBkd262aX71[YQhfW6rboTldpJ2eHSnZDD1cpRqdA>? M4T4RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEW0NVk{Lz5{Mki1OFE6OzxxYU6=
spleen adherent cells M3HTcGFvfGmjcoTodol1cWNiYYPzZZk> MXqyNFAhfWd? MXHBcpRq[XK2aILpeIlkKGWoZnXjeEBqdiCOZYfpd{Bz[XRiYXTqeZZidnRvaX7keYNm\CCjcoTodol1cXNibX;k[Ywhe3CuZXXuJIFlcGW{ZX70JINmdGy|IILld5RqdXWuYYTl[EB4cXSqIIPvcolk[XSnZDDNeIIheG:|dDDpd49t[XSrb36gZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIGLBUnRGWyCycn;keYN1cW:wIHH0JFIxOCC3ZzygbZAhe3SjcoTpcoch\nKxbTDhdpRpemm2aYOgc45{\XRiYX7kJINwdnSrboXl[EB2dmmwdHXydpVxfGWmIIXueIk> Mn3DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6NUSxPVMoRjJ{OEW0NVk{RC:jPh?=
spleen adherent cells MX\BcpRq[XK2aILpeIlkKGG|c3H5 MmL1NlAxKHWp NFG5W3ZCdnSrYYL0bJJqfGmlIHXm[oVkfCCrbjDM[ZdqeyC{YYSgZYRrfX[jboStbY5lfWOnZDDhdpRpemm2aYOgcY9l\Wxic4Ds[YVvKGGmaHXy[Y51KGOnbHzzJJJme3SrbYXsZZRm\CC5aYToJJNwdmmlYYTl[EBOfGJicH;zeEBqe2:uYYTpc44h[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJHRPTmGucHjhJJBzd2S3Y4Tpc44h[XRiMkCwJJVoNCCrcDDzeIFzfGmwZzDmdo9uKGG{dHjybZRqeyCxboPleEBidmRiY3;ueIlvfWWmIIXubY51\XK{dYD0[YQhfW5? NX3FcpdnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4OVQyQTNpPkKyPFU1OTl|PD;hQi=>
spleen adherent cells MoPjRY51cWG{dHjybZRq[yCjc4PhfS=> MUeyNFAhfWd? MV3BcpRq[XK2aILpeIlkKGWoZnXjeEBqdiCOZYfpd{Bz[XRiYXTqeZZidnRvaX7keYNm\CCjcoTodol1cXNibX;k[Ywhe3CuZXXuJIFlcGW{ZX70JINmdGy|IILld5RqdXWuYYTl[EB4cXSqIIPvcolk[XSnZDDNeIIheG:|dDDpd49t[XSrb36gZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFPDVlMh\XiycnXzd4lwdiCjdDCyNFAhfWduIHnwJJN1[XK2aX7nJIZzd21iYYL0bJJqfGm|IH;ud4V1KGGwZDDjc451cW63ZXSgeY5qdnSncoL1dJRm\CC3boTpcC=> NHO0Rlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki1OFE6Oyd-MkK4OVQyQTN:L3G+
spleen adherent cells MlzwRY51cWG{dHjybZRq[yCjc4PhfS=> NXi1TllwOjByIIXn NHH4SHpCdnSrYYL0bJJqfGmlIHXm[oVkfCCrbjDM[ZdqeyC{YYSgZYRrfX[jboStbY5lfWOnZDDhdpRpemm2aYOgcY9l\Wxic4Ds[YVvKGGmaHXy[Y51KGOnbHzzJJJme3SrbYXsZZRm\CC5aYToJJNwdmmlYYTl[EBOfGJicH;zeEBqe2:uYYTpc44h[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGNEWjZiZYjwdoV{e2mxbjDheEAzODBidXesJIlxKHO2YYL0bY5oKG[{b32gZZJ1cHKrdHnzJI9ve2W2IHHu[EBkd262aX71[YQhfW6rboTldpJ2eHSnZDD1cpRqdA>? MlPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ6NUSxPVMoRjJ{OEW0NVk{RC:jPh?=
spleen adherent cells M4mxc2FvfGmjcoTodol1cWNiYYPzZZk> NGTaXI4zODBidXe= M3:wOGFvfGmjcoTodol1cWNiZX\m[YN1KGmwIFzle4l{KHKjdDDh[Ip2fmGwdD3pcoR2[2WmIHHyeIhzcXSrczDtc4RmdCC|cHzl[Y4h[WSqZYLlcpQh[2WubIOgdoV{fGmvdXzheIVlKHerdHigd49vcWOjdHXkJG11[iCyb4P0JIl{d2yjdHnvckBie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gR3hEWjFiZYjwdoV{e2mxbjDheEAzODBidXesJIlxKHO2YYL0bY5oKG[{b32gZZJ1cHKrdHnzJI9ve2W2IHHu[EBkd262aX71[YQhfW6rboTldpJ2eHSnZDD1cpRqdA>? NETZfYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki1OFE6Oyd-MkK4OVQyQTN:L3G+
spleen adherent cells NYPz[ZQ1SW62aXHyeIhzcXSrYzDhd5NigQ>? NEG2W4UzODBidXe= M3fSeWFvfGmjcoTodol1cWNiZX\m[YN1KGmwIFzle4l{KHKjdDDh[Ip2fmGwdD3pcoR2[2WmIHHyeIhzcXSrczDtc4RmdCC|cHzl[Y4h[WSqZYLlcpQh[2WubIOgdoV{fGmvdXzheIVlKHerdHigd49vcWOjdHXkJG11[iCyb4P0JIl{d2yjdHnvckBie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gR3hEWjJiZYjwdoV{e2mxbjDheEAzODBidXesJIlxKHO2YYL0bY5oKG[{b32gZZJ1cHKrdHnzJI9ve2W2IHHu[EBkd262aX71[YQhfW6rboTldpJ2eHSnZDD1cpRqdA>? NFu4eVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki1OFE6Oyd-MkK4OVQyQTN:L3G+
spleen adherent cells M{LO[mFvfGmjcoTodol1cWNiYYPzZZk> M3jld|IxOCC3Zx?= NIjhOHFCdnSrYYL0bJJqfGmlIHXm[oVkfCCrbjDM[ZdqeyC{YYSgZYRrfX[jboStbY5lfWOnZDDhdpRpemm2aYOgcY9l\Wxic4Ds[YVvKGGmaHXy[Y51KGOnbHzzJJJme3SrbYXsZZRm\CC5aYToJJNwdmmlYYTl[EBOfGJicH;zeEBqe2:uYYTpc44h[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJGNZS1J2IHX4dJJme3Orb36gZZQhOjByIIXnMEBqeCC|dHHyeIlv\yCocn;tJIFzfGi{aYTpd{BwdnOndDDhcoQh[2:wdHnueYVlKHWwaX70[ZJzfXC2ZXSgeY51cWx? M{PI[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEW0NVk{Lz5{Mki1OFE6OzxxYU6=
spleen adherent cells NXfSd3dxTnWwY4Tpc44h[XO|YYm= MlG2NlAxKHWp MmHNTY5kemWjc3WgbY4hS0OUMTDlfJBz\XO|aX;uJIlvKEynd3nzJJJifCCjZHr1eoFvfC2rbnT1Z4VlKGG{dHjybZRqeyCvb3TlcEB{eGynZX6gZYRp\XKnboSgZ4VtdHNicnXzeIlufWyjdHXkJJdqfGhic3;ubYNifGWmIF30ZkBxd3O2IHnzc4xifGmxbjDheEAzODBidXesJIlxKHO2YYL0bY5oKG[{b32gZZJ1cHKrdHnzJI9ve2W2IHHu[EBkd262aX71[YQhfW6rboTldpJ2eHSnZDD1cpRqdCC|dIXkfUBmdmRiYomgdXBEWiC{ZXzheIl3\SCkYYPlcIlv NFzxSIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki1OFE6Oyd-MkK4OVQyQTN:L3G+
spleen adherent cells MonzRY51cWG{dHjybZRq[yCjc4PhfS=> MYiyNFAhfWd? Ml;wRY51cWG{dHjybZRq[yCnZn\lZ5QhcW5iTHX3bZMhemG2IHHkbpV3[W62LXnu[JVk\WRiYYL0bJJqfGm|IH3v[IVtKHOybHXlckBi\GincnXueEBk\WyuczDy[ZN1cW23bHH0[YQhf2m2aDDzc45q[2G2ZXSgUZRjKHCxc4SgbZNwdGG2aX;uJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDJUE0y[mW2YTDwdo9lfWO2aX;uJIF1KDJyMDD1[{whcXBic4ThdpRqdmdiZoLvcUBienSqcnn0bZMhd26|ZYSgZY5lKGOxboTpcpVm\CC3bnnueIVzenWydHXkJJVv NIDEd5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki1OFE6Oyd-MkK4OVQyQTN:L3G+
spleen adherent cells MkCxRY51cWG{dHjybZRq[yCjc4PhfS=> NGfuenAzODBidXe= Mmn3RY51cWG{dHjybZRq[yCnZn\lZ5QhcW5iTHX3bZMhemG2IHHkbpV3[W62LXnu[JVk\WRiYYL0bJJqfGm|IH3v[IVtKHOybHXlckBi\GincnXueEBk\WyuczDy[ZN1cW23bHH0[YQhf2m2aDDzc45q[2G2ZXSgUZRjKHCxc4SgbZNwdGG2aX;uJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDDR3I2KGW6cILld5Nqd25iYYSgNlAxKHWpLDDpdEB{fGG{dHnu[{Bnem:vIHHyeIhzcXSrczDvcpNmfCCjbnSgZ49vfGmwdXXkJJVvcW62ZYLyeZB1\WRidX70bYw> NFXqS4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Mki1OFE6Oyd-MkK4OVQyQTN:L3G+
spleen adherent cells NYnIOIIzTnWwY4Tpc44h[XO|YYm= NF;nc4QzPCCqcoO= NHG1e3RKdmO{ZXHz[UBqdiCFQ2KxJJBzd3SnaX6gd5Vz\mGlZTDlfJBz\XO|aX;uJIlvKHOxbnnjZZRm\CCPdHKtd5RqdXWuYYTl[EB{eGynZX6gZYRp\XKnboSgZ4VtdHNiaYPvcIF1\WRiZoLvcUBN\XerczDyZZQh[WSsdY\hcpQucW6mdXPl[EBienSqcnn0bZMhdW:mZXygdJJmNWmwY4XiZZRm\CC5aYToJFAvOSC2bzCwMlMhfU1iY3;tdI92dmRiYnXmc5JmKHO2aX31cIF1cW:wIIfpeIghe2:waXPheIVlKE22YjDmc5IhOjRiaILzJIJ6KG[ub4egZ5l1d21? NXvhNHk4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4OVQyQTNpPkKyPFU1OTl|PD;hQi=>
RAW264.7 NGXoTlJCdnSraX7mcIFudWG2b4L5JIF{e2G7 NWjqeIlROjRiaILz MlTqRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44h[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDt[ZRpd2RuIFnDOVAhRSBzIN88UU4> MnTVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNzMke4PFYoRjJ|MUK3PFg3RC:jPh?=
RAW264.7 NX7WXlBJSW62aXnu[oxidW2jdH;yfUBie3OjeR?= NVnSZmpuOjRiaILz NGXPdo1CdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgWG5H[WyyaHGgdJJw\HWldHnvckBqdmO3YnH0[YQh\m:{IEK0JIhzeyCkeTDFUGlUSSxiSVO1NEA:KDBwODFOwG0v NUTwfVR[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN|Q1ODdpPkKzNlM1PDB5PD;hQi=>
RAW264.7 MnjWRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? NHLtRZMzPCCqcoO= MUHBcpRqcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRibnn0dolkKG:6aXTlJIdmdmW{YYTpc44hcW6ldXLheIVlKG[xcjCyOEBpenNuIFnDOVAhRSBzIN88UU4> NXHtTmxNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyN|Q1ODdpPkKzNlM1PDB5PD;hQi=>
RAW264.7 NF3k[IxCdnSraX7mcIFudWG2b4L5JIF{e2G7 MUXBcpRqNWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIH7peJJq[yCxeHnk[UBxem:mdXP0bY9vKGK7IFfybYV{eyC{ZXHjeIlwdiCkYYPl[EBu\XSqb3SsJGlEPTBiPTCxJO69VS5? M3P3flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkO3N|Y{Lz5{NU[zO|M3OzxxYU6=
MCF7 MXrDfZRwfG:6aXPpeJkh[XO|YYm= M1KxeVczKGi{cx?= M{nIWmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYX\0[ZIhPzJiaILzJIJ6KE2WUz;QUXMh[XO|YYmsJGlEPTBiPTCwMlE2OyEQvF2u NFWxSmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe1OlI6QSd-MkW3OVYzQTl:L3G+
MCF7 MV;GeY5kfGmxbjDhd5NigQ>? MWSyOEBpenN? NUi5ZWdyUW6qaXLpeIlwdiCxZjDIV3A6OCCrbjDoeY1idiCPQ1[3JINmdGy|IHHzd4V{e2WmIHHzJJBCc3RiZHXndoFl[XSrb36gZZQhPSC2aX3ld{BKSzVyIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd3NkK5PUc,OjV5NU[yPVk9N2F-
MCF7 MlriSpVv[3Srb36gZZN{[Xl? MWGyOEBpenN? M4noR2lvcGmkaYTpc44hd2ZiSGPQPVAhcW5iaIXtZY4hVUOINzDj[YxteyCjc4Pld5Nm\CCjczDI[ZIzKGSnZ4Lh[IF1cW:wIHH0JFUhfGmvZYOgTWM2OCCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MknpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NU[yPVkoRjJ3N{W2Nlk6RC:jPh?=
MCF7 M{TYVGZ2dmO2aX;uJIF{e2G7 MnzoNlQhcHK| NGT2O2NKdmirYnn0bY9vKG:oIFjTVFkxKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOgR4RsPiCmZXfyZYRifGmxbjDheEA2KHSrbXXzJGlEPTBiYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MoWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NU[yPVkoRjJ3N{W2Nlk6RC:jPh?=
MCF7 M4nDfGZ2dmO2aX;uJIF{e2G7 Mn\tNlQhcHK| MlTDTY5pcWKrdHnvckBw\iCKU2C5NEBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IGLh[kBl\We{YXTheIlwdiCjdDC1JJRqdWW|IFnDOVAh[W[2ZYKgNlQhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTd3NkK5PUc,OjV5NU[yPVk9N2F-
MCF7 NXL3WoZQTnWwY4Tpc44h[XO|YYm= MXiyOEBpenN? MV;DbIFv\2ViaX6gR4RkOzdiZYjwdoV{e2mxbjDpckBpfW2jbjDNR2Y4KGOnbHzzJIF1KDVidHnt[ZMhUUN3MDDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NELBflI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUe1OlI6QSd-MkW3OVYzQTl:L3G+
MCF7 NWjDeo1RTnWwY4Tpc44h[XO|YYm= M1LPbVI1KGi{cx?= NF\nZ4tEcGGwZ3WgbY4heDJ|IHX4dJJme3Orb36gbY4hcHWvYX6gUWNHPyClZXzsd{BifCB3IITpcYV{KEmFNUCgZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> Mlz3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV5NU[yPVkoRjJ3N{W2Nlk6RC:jPh?=
MCF7 M4jYd2Z2dmO2aX;uJIF{e2G7 NV\BOmljOjRiaILz MlPlTY5pcWKrdHnvckBw\iCKU2C5NEBqdiCqdX3hckBOS0Z5IHPlcIx{KGG|c3Xzd4VlKGG|IHTpd5J2eHSrb36gc4YhcW62ZYLhZ5Rqd25id3n0bEBE\GN|NzDheEA2KHSrbXXzJGlEPTBiYX\0[ZIhOjRiaILzJIJ6KGOxLXntcZVvd3C{ZXPpdIl1[XSrb36gZZN{[Xl? NUHIUlZ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3OVYzQTlpPkK1O|U3Ojl7PD;hQi=>
MCF7 MkjsSpVv[3Srb36gZZN{[Xl? NWDRb3pFOjRiaILz NHLQV|lKdmirYnn0bY9vKG:oIFjTVFkxKGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOg[Il{enWydHnvckBw\iCrboTldoFkfGmxbjD3bZRpKHB{MzDheEA2KHSrbXXzJGlEPTBiYX\0[ZIhOjRiaILzJIJ6KGOxLXntcZVvd3C{ZXPpdIl1[XSrb36gZZN{[Xl? NWfD[|BZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3OVYzQTlpPkK1O|U3Ojl7PD;hQi=>
SGC7901 MVTBcpRq[2GwY3XyJIF{e2G7 M1[yelQ5KGi{cx?= NWPRUJBuSW62aXPhcoNmeiCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJHNISzd7MEGgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCC|dYL2bZZidCCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOC5zNTFOwG0v M2OwR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEGyPVY3Lz5{NUixNlk3PjxxYU6=
SMMC7721 NVHpRVhvSW62aXPhcoNmeiCjc4PhfS=> MlPBOFghcHK| M3PpZmFvfGmlYX7j[ZIh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTUW1EPzd{MTDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKHO3co\peoFtKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAxNjV6IN88UU4> M{KxWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEGyPVY3Lz5{NUixNlk3PjxxYU6=
MGC803 MlTSRY51cWOjbnPldkBie3OjeR?= M2r2bFQ5KGi{cx?= MXjBcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVfDPFA{KGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygd5Vzfmm4YXygZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGuOVUh|ryPLh?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPThzMkm2Okc,OjV6MUK5OlY9N2F-
HepG2 MVHBcpRq[2GwY3XyJIF{e2G7 MXq0PEBpenN? MVHBcpRq[2GwY3XyJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSHXwS|Ih[2WubIOgZZN{\XO|ZXSgZZMh[2WubDDzeZJ3cX[jbDDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iND6wNUDPxE1w NX7EbG1ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NVI6PjZpPkK1PFEzQTZ4PD;hQi=>
A549 M3zkOmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NUPlUFkxPDhiaILz NV75NnppSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBx[WOuaYThfIVtNXKnc3nzeIFvfCCqdX3hckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxKD1iMj6wNUDPxE1w NWTsfFB3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke2OFc{PjlpPkK3OlQ4OzZ7PD;hQi=>
A549 NYnXUm9VSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NELKcpY1QCCqcoO= NY\keGt{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOi5yMjFOwG0v M3O4clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkS3N|Y6Lz5{N{[0O|M3QTxxYU6=
MCF7 M4noOWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NHzEVpY1QCCqcoO= NF\sN|ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUCgQUAzNjF|IN88UU4> M2X4b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NkS3N|Y6Lz5{N{[0O|M3QTxxYU6=
PANC1 NXvwU2tUSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MkjPOFghcHK| MWrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFCDTlOxJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiClZXzsJJZq[WKrbHn0fUBi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyIE2gNk41QCEQvF2u MknLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd4NEezOlkoRjJ5NkS3N|Y6RC:jPh?=
LNCAP NWjoXIZOTnWwY4Tpc44h[XO|YYm= NF3OcVQ2KHWP MmG0NlQhcHK| NF\zTWZCdnSjZ3;ubZN1KGGldHn2bZR6KGG2IFHSJIlvKGi3bXHuJGxPS0GSIHPlcIx{KGG|c3Xzd4VlKGG|IIP1dJBz\XO|aX;uJI9nKESKVD3pcoR2[2WmIILlZ4VxfG:{IITyZY5{[3KrcITpc45idCCjY4Tpeol1gSCjdDC1JJVOKGGodHXyJFI1KGi{czDifUBlfWGuIHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzl7NEezNUc,Ojd7OUS3N|E9N2F-
A549 M2XlN2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWKxO|hXPiCmYYnz NUO1S3cyT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hSTV2OTDj[YxteyCvZXHzeZJm\CCndnXyfUAzKGi{czDveoVzKDZiZHH5d{BjgSCuaY\lJINmdGxiaX3h[4lv\yCkYYPl[EBu\XSqb3SsJGlEPTBiPTCwMlY6KM7:TT6= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ{MUm0N{c,Ojh4MkG5OFM9N2F-
HEK293 MlPtSpVv[3Srb36gZZN{[Xl? NXPkZms3OTBidV2= MnezNlAhdWmwcx?= M4jYW2lvcGmkaYTpc44hd2ZiR3HsdIhidyCrboTldoFkfGmxbjD3bZRpKEeIUD3meZNm\CCUR2OxO{ApfW6tbn;3ckBwemmpaX6pJIRmdHSjTjDteZRidnRiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBTT1NzNzDk[Yx1[U5ibYX0ZY51KGyxY3HsbZpifGmxbjDheEBkgXSxcHzhd40h[XRiMUCgeW0hfXBidH:gNlAhdWmwczDifUBkd26ob3PhcEBucWO{b4Pjc5Bq[yCjbnHsfZNqew>? NX;ZdXlNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2NlE6PDNpPkK4OlIyQTR|PD;hQi=>
HEK293 M2j5TmZ2dmO2aX;uJIF{e2G7 MmTGNVAxKHWP M{LISFUhdWmwcx?= NELmcVZKdmirYnn0bY9vKG:oIFfhcJBp[TBiaX70[ZJi[3Srb36ge4l1cCCJRmCt[pV{\WRiUlfTNVchMHWwa37ve44hd3KrZ3nuLUBl\Wy2YV6gcZV1[W62IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hWkeVMUeg[IVtfGGQIH31eIFvfCCub3PhcIl7[XSrb36gZZQh[3m2b4DsZZNuKGG2IEGwNEB2VSC3cDD0c{A2KG2rboOgZpkh[2:wZn;jZYwhdWmlcn;zZ49xcWNiYX7hcJl{cXN? M{\QUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkKxPVQ{Lz5{OE[yNVk1OzxxYU6=
HEK293 M4DMc2Z2dmO2aX;uJIF{e2G7 MkXuNVAhfU1? MUW1JI1qdnN? NUTvSWh2UW6qaXLpeIlwdiCxZjDHZYxxcGGxIHnueIVz[WO2aX;uJJdqfGhiR1\QMYZ2e2WmIGLHV|E4KCi3bnvuc5dvKG:{aXfpckkh\GWudHHOJI12fGGwdDDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKFKJU{G3JIRmdHSjTjDteZRidnRibH;jZYxqgmG2aX;uJIF1KGO7dH;wcIF{dSCjdDCxNEB2VSC3cDD0c{A2KG2rboOgZpkh[2:wZn;jZYwhdWmlcn;zZ49xcWNiYX7hcJl{cXN? NVHMcHVNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2NlE6PDNpPkK4OlIyQTR|PD;hQi=>
MIAPaCa2 NWPGOmhWSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M1TxU|czKGi{cx?= M2rBb2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVnBVIFE[TJiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFAvPDZizszNMi=> NXPmPZhyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2PFgzQDFpPkK4Olg5OjhzPD;hQi=>
SKBR3 M4LvOmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NYjyNY9MPzJiaILz MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFONQmKzJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSByLkeyJO69VS5? M4nuUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki4NlgyLz5{OE[4PFI5OTxxYU6=
SKOV3 MkG0RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NU\vc3FtPzJiaILz M2rncmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1vPWlMh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEGuNVYh|ryPLh?= M4ixflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki4NlgyLz5{OE[4PFI5OTxxYU6=
MDA-MB-231 NHuyV2hCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MoXoO|IhcHK| M1S0ZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwJF0hOS5|MjFOwG0v M1zDOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6Nki4NlgyLz5{OE[4PFI5OTxxYU6=
A549 NFWwOXlCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MYK3NkBpenN? MoTMRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCDNUS5JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSBzLkW2JO69VS5? MlzLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4OEiyPFEoRjJ6Nki4NlgyRC:jPh?=
BJ NYHFS5MxS3m2b4TvfIlkcXS7IHHzd4F6 NFvhe2s4OiCqcoO= MXTDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDCTkBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3MDC9JFIvPzRizszNMi=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ6OEK4NUc,Ojh4OEiyPFE9N2F-
NCI-H460 NWfySmd1TnWwY4Tpc44h[XO|YYm= MlT6NUB2VQ>? NHf0bVYyOiCqcoO= MXjBZ5RqfmG2aX;uJI9nKEiVRkGgbY4hcHWvYX6gUmNKNUh2NkCgZ4VtdHNiYYPz[ZN{\WRiYYOgeZBz\We3bHH0bY9vKG:oIFjTVFI2KHC{b4TlbY4hdGW4ZXzzJIF1KDFidV2gZYZ1\XJiMUKgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd|N{mxOkc,Ojh5M{e5NVY9N2F-
NCI-H460 Mor2SpVv[3Srb36gZZN{[Xl? M{nSeVEhfU1? NYj0TlVQOTJiaILz NGC2W|NC[3SrdnH0bY9vKG:oIFjTSlEhcW5iaIXtZY4hVkOLLVi0OlAh[2WubIOgZZN{\XO|ZXSgZZMhfXC{ZXf1cIF1cW:wIH;mJGhUWDdyIIDyc5RmcW5ibHX2[Yx{KGG2IEGgeW0h[W[2ZYKgNVIhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? NVzDXYR4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3N|c6OTZpPkK4O|M4QTF4PD;hQi=>
NCI-H1299 MVvDfZRwfG:6aXPpeJkh[XO|YYm= MU\DfZRwfG:6aXPpeJkhcW5iaIXtZY4hVkOLLVixNlk6KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDj[YxtKH[rYXLpcIl1gSxiSVO1NEA:KDFizszNMi=> NGXKXHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEe1OFQ4OCd-Mki3OVQ1PzB:L3G+
BCP-ALL NUj3XZhFS3m2b4TvfIlkcXS7IHHzd4F6 Mn3PR5l1d3SxeHnjbZR6KGmwIHj1cYFvKEKFUD3BUGwh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6NCCLQ{WwJF0hOSEQvF2u NIC4cIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEe1OFQ4OCd-Mki3OVQ1PzB:L3G+
T-ALL MUHDfZRwfG:6aXPpeJkh[XO|YYm= NHHZd3REgXSxdH;4bYNqfHliaX6gbJVu[W5iVD3BUGwh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6NCCLQ{WwJF0hOSEQvF2u MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDd3NES3NEc,Ojh5NUS0O|A9N2F-
Daudi NI\ITGhEgXSxdH;4bYNqfHliYYPzZZk> NGrDbIVEgXSxdH;4bYNqfHliaX6gbJVu[W5iRHH1[Ikh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6NCCLQ{WwJF0hOSEQvF2u NYWyepRjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki3OVQ1PzBpPkK4O|U1PDdyPD;hQi=>
HL60 NFzqcYREgXSxdH;4bYNqfHliYYPzZZk> MVXDfZRwfG:6aXPpeJkhcW5iaIXtZY4hUEx4MDDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImsJGlEPTBiPTCxJO69VS5? MmTVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh5NUS0O|AoRjJ6N{W0OFcxRC:jPh?=
TC32 M1LGW5FJXFNiYYPzZZk> NIjuZoxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiVFOzNkBk\Wyucx?= MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
DAOY MUnxTHRUKGG|c3H5 M3XGTJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCGQV;ZJINmdGy| MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SJ-GBM2 NGiw[XdyUFSVIHHzd4F6 MkK4dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIO= M2PQblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 NWjmSXAzeUiWUzDhd5NigQ>? MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSTZ5MzDj[Yxtew>? M{\1c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC MkDJdWhVWyCjc4PhfS=> MoHndWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= Ml7GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 NXH4WZlteUiWUzDhd5NigQ>? MYHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHN? Mlr1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
NB-EBc1 M3fuVJFJXFNiYYPzZZk> NF[xeWdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKtSWJkOSClZXzsdy=> MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Saos-2 MojZdWhVWyCjc4PhfS=> NYLLfZF7eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPhc5MuOiClZXzsdy=> NIrQPYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-SH MnP0dWhVWyCjc4PhfS=> MkO3dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tV2gh[2WubIO= M1;EN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 NETMNY9yUFSVIHHzd4F6 M1jYTZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQkG2OFMh[2WubIO= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
LAN-5 M2jrd5FJXFNiYYPzZZk> Mn72dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKEyDTj21JINmdGy| M4iwOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-12 NWe3[JdPeUiWUzDhd5NigQ>? M1rOSJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCEVD2xNkBk\Wyucx?= M4q3TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 NW[3dIFTeUiWUzDhd5NigQ>? Mnu2dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFKqMUigZ4VtdHN? MljyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
OHS-50 NWfYTI9ZeUiWUzDhd5NigQ>? M4jlSZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCRSGOtOVAh[2WubIO= NUXXSGdoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
RD M1nlfpFJXFNiYYPzZZk> MUPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM> NILac2I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) NXO0cWs1eUiWUzDhd5NigQ>? NYLtWHQ1eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF3HJFY{KCh4LWTHJHIqKGOnbHzz MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh30 NFXMfFByUFSVIHHzd4F6 MVjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh|MDDj[Yxtew>? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 MnX2dWhVWyCjc4PhfS=> NW\UfIxueUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoOFEh[2WubIO= NEXuT|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB1643 NW[5ZllveUiWUzDhd5NigQ>? MWTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDORlE3PDNiY3XscJM> M1nEOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673 M2fwfJFJXFNiYYPzZZk> MXTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDBOlc{KGOnbHzzLS=> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC MV7xTHRUKGG|c3H5 NV\LXJIzeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= NUHDSYM6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-12 MljTdWhVWyCjc4PhfS=> NGnidopyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBDXC1zMjDj[Yxtew>? Mn7QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
LAN-5 MlLUdWhVWyCjc4PhfS=> M3K2NpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGxCVi13IHPlcIx{ NFqx[nM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
DAOY MV;xTHRUKGG|c3H5 MXnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDERW9[KGOnbHzz NEHnUo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB-EBc1 NUDWcYdYeUiWUzDhd5NigQ>? MkLxdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgUmIuTUKlMTDj[Yxtew>? NWD6RpRTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SJ-GBM2 M1zOWZFJXFNiYYPzZZk> NYLtO|VleUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= Mny2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 MVPxTHRUKGG|c3H5 M1HmbpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGJVNTN5IHPlcIx{ M1KzXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32 MoPNdWhVWyCjc4PhfS=> M{XGfJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHREOzJiY3XscJM> NFHJRVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
MG 63 (6-TG R) MX\xTHRUKGG|c3H5 NY\GWYJbeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHN? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
fibroblast cells M2fUVpFJXFNiYYPzZZk> NVzR[JBCeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5Ih[2:wdILvcEBJcCC5aXzkJJR6eGViZnnido9jdGG|dDDj[Yxtew>? NGPFV2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
U-2 OS MnvMdWhVWyCjc4PhfS=> NX7CXJFMeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXS1{IF;TJINmdGy| NVjpdWtvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh41 MnvqdWhVWyCjc4PhfS=> MnHBdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog1OSClZXzsdy=> MmDvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
RD MYPxTHRUKGG|c3H5 Ml3adWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVmQh[2WubIO= NXTZcY04RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Rh18 M13UXpFJXFNiYYPzZZk> MoLLdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVogyQCClZXzsdy=> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh30 MYnxTHRUKGG|c3H5 Mlf1dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVog{OCClZXzsdy=> MorRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
Saos-2 M3jIV5FJXFNiYYPzZZk> NEPCRYxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBU[W:|LUKgZ4VtdHN? MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
OHS-50 MoO4dWhVWyCjc4PhfS=> MY\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDPTHMuPTBiY3XscJM> NVfnZnN1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SK-N-SH MmDEdWhVWyCjc4PhfS=> MULxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNVOKIHPlcIx{ NYPmNWU2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
HCT116 MnqxRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M{DRN|Q5KGi{cx?= MnLpRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCKQ2SxNVYh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IEWuNlYh|ryPLh?= MkHCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OE[5OVQoRjJ7NEi2PVU1RC:jPh?=
HepG2 MX;BcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NXLHd4hDPDhiaILz MYDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEincFeyJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVAhRSB4LkG3JO69VS5? NXzC[2FwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0PFY6PTRpPkK5OFg3QTV2PD;hQi=>
A549 Mk\qRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NYXGO5hGPDhiaILz NUWw[W9qSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBOVQ6KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC2MlU6KM7:TT6= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR6Nkm1OEc,Ojl2OE[5OVQ9N2F-
MCF7 MkjNRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NUHQS2NuPDhiaILz NV3sOppOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTBiPTC2Mlg1KM7:TT6= M4LyR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEi2PVU1Lz5{OUS4Olk2PDxxYU6=
HEK293 NVnBXXN5S3m2b4TvfIlkcXS7IHHzd4F6 M3vwSGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KEiHS{K5N{Bk\WyuczCoR28uSUSGOl3BY|AxPyl9IFPDOVAh[nliY3XscEB3cWGkaXzpeJkh[XO|YYmgbY4hTE2HTTCoNVAmKE[EUzmgcYVlcWFidYPpcochXENicHzheIV{NCCkeTDS[ZNignW{aX6gSkg2PjBxNUmwLUwhS0N3MDC9JFAvQDN5IN88UU4> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyzNFE6QDJxJ{7DbGVOSkx:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
HIF-1α; 

PubMed: 25383959     


Celastrol enhanced HIF-1α expression in multiple cell lines. MCF-7, HeLa, PC-3 and H1299 cells were treated with the indicated doses of Celastrol for 12 h under 3% hypoxia, and western blotting was used to detect the HIF-1 proteins.

Akt / p-Akt / p-p70S6K ; 

PubMed: 25383959     


Celastrol induced AKT/p70S6K activation under normoxia and hypoxia. HepG2 cells were challenged with the indicated doses of Celastrol for 6 h under normoxia or hypoxia. The protein levels were analyzed by western blotting with the corresponding antibodies. 

PARP / p53 / p21 / cIAP1 / Bcl-xl / Bcl-2 ; 

PubMed: 25383959     


Celastrol transiently activates p53 under normoxia but persistently activates p53 under hypoxia. HepG2 cells were cultured in normoxia or hypoxia with or without 4 µM Celastrol for the indicated times. The proteins were detected by western blotting. 

IκBα / p-IKKα/β ; 

PubMed: 28388439     


Inhibition of TNFα-induced IKKα/β phosphorylation and IκBα degradation by celastrol. Cells treated with celastrol and/or TNFα (20 ng/mL) for 30 min were analyzed by western blotting (WB).

iNOS / COX-2 / Arg-1 ; 

PubMed: 29040966     


Effects of celastrol on the expression of iNOS, COX‐2 and arginase-1 in LPS-stimulated RAW264.7 cells. After 24 h treatment with LPS and/or celastrol, the cellular proteins were analyzed by Western blotting with specific antibodies and GAPDH (as loading control). The blots (n = 3) were quantified by a densitometric method. Representative blots were shown

Chk2 / p-Chk2 / Cyclin B1 / Cdc25c / p-Cdc25c ; 

PubMed: 31053160     


U251, U87-MG and C6 cells were treated with celastrol (0, 0.3, 1, 3 and 10 μM) for 24 h. The levels of cell cycle-associated protein expressions were analyzed by western blotting. β-actin was used as an internal control.

25383959 28388439 29040966 31053160
Immunofluorescence
p21 / Nur77 ; 

PubMed: 28388439     


Colocalization of Nur77 with p62. Representative images illustrate colocalization of transfected endogenous Nur77 (E) or transfected GFP-Nur77 (F) with p62 in HepG2 cells after treatment with 4 μM celastrol and 20 ng/mL TNFα for 1 hr, analyzed by immunostaining. Scale bar, 10 μm.

28388439
Growth inhibition assay
Cell viability; 

PubMed: 29040966     


Cytotoxicity of celastrol. Following 48 h treatment, RAW264.7 cells were determined for the cell viability by a colorimetric MTT assay relative to the untreated controls (n = 3). **, p < 0.01 (Celastrol vs Control).

29040966
体内研究 Celastrol (3 mg/kg)处理携带PC-3肿瘤的雄性裸鼠,显著抑制肿瘤的生长(抑制高达70%),并提高p27水平和Bax水平。Celastrol(3 mg/kg)处理携带PC-3肿瘤的雄性裸鼠,导致产生更多凋亡肿瘤细胞,并出现各种PARP裂解片段。Celastrol (3 mg/kg) 处理携带C4-2B肿瘤的裸鼠,抑制35%肿瘤,降低蛋白酶体活性,也降低AR蛋白表达。[1] Celastrol (3 mg/kg)处理小鼠,强抑制关节肿胀和和其他反应性关节炎。Celastrol(0.2 mg/kg) 处理大鼠,显著提高记忆,学习,和精神运动活性。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

纯化的20S 蛋白酶体活性抑制实验:

纯化的兔20S蛋白酶体(0.1 μg)与40 μM 各种荧光多肽底物在100 μL实验buffer (20 mM Tris-HCl (pH 7.5))中一起温育,其中含不同浓度的Celastrol或DMSO,在37°C下温育2小时,然后测量每种蛋白酶体活性的抑制情况。
细胞实验:[4]
- 合并
  • Cell lines: RPMI 8226, KATOIII, UM-SCC1, U251MG 和 MDA-MB-231 细胞
  • Concentrations: 5 μM
  • Incubation Time: 2 小时
  • Method: 通过MTT摄取法测定Celastrol作用于各种人类肿瘤细胞系的抗增殖作用。5×103细胞与Celastrol按一式三份在96孔板中 37°C下温育。然后每孔加入MTT溶液。在37°C下温育2小时后,加入提取缓冲液(20% SDS, 50%二甲基甲酰胺),细胞在37°C下温育过夜,然后使用Tecan酶标仪在570 nm处测量光密度。
    (Only for Reference)
动物实验:[1]
- 合并
  • Animal Models: 携带 C4-2B 肿瘤的裸鼠
  • Dosages: 3 mg/kg
  • Administration: 腹腔注射
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 90 mg/mL (199.72 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 450.61
化学式

C29H38O4

CAS号 34157-83-0
储存条件 粉状
溶于溶剂
别名 Tripterine

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项
免费分装抑制剂

Proteasome Signaling Pathway Map

相关Proteasome产品

Tags: 购买Celastrol (NSC 70931) | Celastrol (NSC 70931)供应商 | 采购Celastrol (NSC 70931) | Celastrol (NSC 70931)价格 | Celastrol (NSC 70931)生产 | 订购Celastrol (NSC 70931) | Celastrol (NSC 70931)代理商
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID