Entinostat (MS-275)

For research use only. Not for use in humans.

目录号：S1053 别名： SNDX-275 中文名称：恩替诺特

CAS No. 209783-80-2

Entinostat (MS-275, SNDX-275)强烈抑制HDAC1和HDAC3，无细胞试验中IC50分别为0.51 μM和1.7 μM，抑制作用强于HDACs 4， 6， 8，和10。Entinostat可诱导自噬和凋亡。Phase 3。

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10mM (1mL in DMSO)	RMB 746.45	现货
10mg	RMB 794.43	现货
50mg	RMB 2234.07	现货
200mg	RMB 6301.79	现货
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客户使用Selleck生产的Entinostat (MS-275)发表文献337篇：

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产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述

靶点

HDAC1 ^[2] (Cell-free assay)	HDAC3 ^[2] (Cell-free assay)
0.51 μM	1.7 μM

体外研究

MS-275通过作用于2′-氨基而抑制HDACs。MS-275作用于 K562细胞，诱导 p21^WAF1/CIP1和凝溶胶蛋白的累积。MS-275作用于A2780细胞，可以降低S期细胞，提高G1期细胞。 MS-275通过抑制HAD而抑制人类肿瘤细胞系，包括 A2780, Calu-3, HL-60, K562, St-4, HT-29, KB-3-1, Capan-1, 4-1St和 HCT-15细胞增殖， IC50 为41.5 nM到4.71 μM。^[1]MS-275抑制HDACs，作用于HDAC1和 HDAC3时IC50 分别为0.51 μM和1.7 μM。而对其他的HDACs没有抑制效果，如HDAC4, 6, 8和10。^[2]MS-275有效抑制人类白血病和淋巴癌细胞，包括U937, HL-60, K562, 和Jurkat。MS-275可以诱导U937细胞p21CIP1/WAF1 调节的生长和变异Marker (CD11b)的表达。MS-275降低cyclin D1和抗凋亡蛋白Mcl-1与XIAP的表达。^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
SCC-3	M1fQPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NF7nRYlKSzVyPUCuNFYyKM7:TR?=	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
ALL-PO	NYL5WpZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M{T2O2lEPTB;MD6wOlM2PSEQvF2=	NWTXOWN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
697	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MoS1TWM2OD1yLkC5PVc3KM7:TR?=	NH7KRlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
NCI-H748	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MWjJR\|UxRTBwMUCzN\|Qh\|ryP	NWDIb4FRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
NKM-1	NUnwNYJPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Ml;CTWM2OD1yLkGwPVEzKM7:TR?=	MnTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
ES1	MmfGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NGe3PGVKSzVyPUCuNVEzPTVizszN	NYDV[4t2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
NCI-H1963	M1XNNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NHXEXmNKSzVyPUCuNVE2PzlizszN	MoPRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
NCI-H1417	NIfnTWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MV;JR\|UxRTBwMUK5O\|Qh\|ryP	M{W0[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
NEC8	MlfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MkHDTWM2OD1yLkGzOVI4KM7:TR?=	NWjadYhGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
CRO-AP2	MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MUDJR\|UxRTBwMU[4PFkh\|ryP	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
A3-KAW	M4rZTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M3jzc2lEPTB;MD6xO\|YzPyEQvF2=	NH65N5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
SF539	NWLSTmVlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M3zQemlEPTB;MD6xPVU6OyEQvF2=	Mon0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
NOS-1	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MUnJR\|UxRTBwMUm2NVkh\|ryP	NVLWc41VRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
NTERA-S-cl-D1	MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M37rTmlEPTB;MD6yNFEyOyEQvF2=	NG\USZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
COR-L88	M1jaW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MkfpTWM2OD1yLkKyPVU6KM7:TR?=	NUP6T5ViRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
EM-2	NU\UcZdyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NUjyU3I3UUN3ME2wMlI1ODd7IN88US=>	MkLHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
KARPAS-45	MlzrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NHXGWWdKSzVyPUCuNlc5OzNizszN	NWPKcnlzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
DSH1	MoHES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MYjJR\|UxRTBwMki3NFgh\|ryP	M3HNblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
HT-144	Ml\sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NG\KeXRKSzVyPUCuN\|AzPTZizszN	M3rYfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
ATN-1	NX7Ld3Q2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NVPXSnd3UUN3ME2wMlMxPTd4IN88US=>	NHO5UoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
HEL	M{nsUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NFv0W3pKSzVyPUCuN\|E{PDhizszN	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
NB12	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M17h[GlEPTB;MD6zNVc2PiEQvF2=	NIfBZ3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
LU-139	MnTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MVvJR\|UxRTBwM{O1NUDPxE1?	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
J-RT3-T3-5	MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M3nqVmlEPTB;MD6zN\|cyPiEQvF2=	M{\lZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
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PF-382	NGrJdHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MmnmTWM2OD1zLkGxOVE{KM7:TR?=	NFTiVG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
LC-1F	NUPEe\|N[T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Ml[zTWM2OD1zLkGyNFA4KM7:TR?=	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
OVCAR-4	M1m4R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M3y2fWlEPTB;MT6xN\|E3PSEQvF2=	NEnWUIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
A4-Fuk	NFLGRpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M1LLV2lEPTB;MT6xOVM3PCEQvF2=	M37PfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
HCC2218	MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NGLycFlKSzVyPUGuNVY3PDFizszN	MoixQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
HAL-01	M1nsc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NIXPXpFKSzVyPUGuNVY6PDNizszN	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
IST-MEL1	MnXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NHPzSm1KSzVyPUGuNVc3PTlizszN	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
NCI-H719	NEezTIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NGHISmRKSzVyPUGuNVc5QThizszN	Mk\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
EVSA-T	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MnS1TWM2OD1zLkG4NVE1KM7:TR?=	NEi3[mY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
SK-NEP-1	NH7tbpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NGrDe5lKSzVyPUGuNlAzPjZizszN	NInJWYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
OCUB-M	M{XpUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MnTqTWM2OD1zLkKxOFg6KM7:TR?=	M2\BNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
MEG-01	M1zhN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MnjXTWM2OD1zLkKyNVE5KM7:TR?=	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
no-10	M3m3Z2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NGnjTllKSzVyPUGuNlMyOTJizszN	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
MHH-CALL-2	NF2xO4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M3Hoc2lEPTB;MT6yOFczOSEQvF2=	NWTyS49FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
SK-N-DZ	M{P0cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NGf3e\|RKSzVyPUGuNlQ4PzZizszN	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
SCLC-21H	Mn;5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MVnJR\|UxRTFwMk[0O\|gh\|ryP	M3rMUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
CTV-1	MoPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M17XeWlEPTB;MT6yO\|QzPSEQvF2=	NGXCXos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
NB1	MmG2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MXfJR\|UxRTFwMke3N\|Ih\|ryP	M{W1TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
NCI-H64	NVzUWlF3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M2nke2lEPTB;MT6yPFQ3OiEQvF2=	M{[yVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
MDA-MB-134-VI	Mn3lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MkHtTWM2OD1zLkK4OVc4KM7:TR?=	NYfRZ5JLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
LB2241-RCC	NVX4d5JqT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MWTJR\|UxRTFwMki2OlMh\|ryP	M3j1dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
8-MG-BA	NFPqWIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			Mk\BTWM2OD1zLkK4PFY3KM7:TR?=	NWf6NYxlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
LP-1	NVfnZlNxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M4TxdGlEPTB;MT6yPVk1PyEQvF2=	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
LS-411N	Mm\hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M1jGSWlEPTB;MT6zNFk6QCEQvF2=	M1HMOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
CAL-148	M36we2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M4jUSWlEPTB;MT6zNlU1OiEQvF2=	NY[2V5lxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
NCI-H2171	MmHrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M17VOmlEPTB;MT6zOFUxOiEQvF2=	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
JiyoyeP-2003	NYDBdFdtT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NFntbJJKSzVyPUGuN\|U{QSEQvF2=	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
NCI-H2107	M3vnT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MkfqTWM2OD1zLkO1PFg{KM7:TR?=	MkjCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
BB30-HNC	MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MWrJR\|UxRTFwM{i5O\|gh\|ryP	NXHocW5yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
K-562	M3;0WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MkHpTWM2OD1zLkO5NlE6KM7:TR?=	NWi3VoNkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
PSN1	NVvnXYx4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NFTySpNKSzVyPUGuOFIzQDdizszN	M3f5O\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
HCC2157	M3LEeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M{Czc2lEPTB;MT60NlY6OSEQvF2=	NXvvbY9GRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
SBC-1	NXThV4tnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MnnZTWM2OD1zLkSyO\|QyKM7:TR?=	NInjPY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
MC116	M3\yVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NFHuWWlKSzVyPUGuOFM3OTVizszN	NYrDZ\|VORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
KARPAS-422	NX;lTVlzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MojCTWM2OD1zLkS1N\|U5KM7:TR?=	NXHxfHJbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
LB996-RCC	M{HTeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M2LOfWlEPTB;MT60O\|ExOyEQvF2=	Ml34QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
MSTO-211H	M4rIXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MY\JR\|UxRTFwNEe5PFch\|ryP	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
BT-474	MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NGXiRW1KSzVyPUGuOVE4PjRizszN	NUHuXJl3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
A388	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MUHJR\|UxRTFwNUG5OFUh\|ryP	NUHJRnFNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
SJSA-1	MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M33BUmlEPTB;MT61NlI3KM7:TR?=	M{LUTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
COLO-829	NV3lW3dwT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NXToOYJvUUN3ME2xMlU{PTZ2IN88US=>	NGfMVmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
KM-H2	NVnzZolPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MnHtTWM2OD1zLkW2Olch\|ryP	M3vVTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
GR-ST	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NYDlOpFXUUN3ME2xMlU3QDJizszN	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
RPMI-8866	M3u4emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MoXYTWM2OD1zLk[wNVQ1KM7:TR?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
KG-1	NUH1fGI5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXXJR\|UxRTFwNkG5NFEh\|ryP	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
NCI-H82	M2TGOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NYexRXhtUUN3ME2xMlY{PDB4IN88US=>	NFK3coc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
LB1047-RCC	NYDlPZNjT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MnzRTWM2OD1zLk[zOFU6KM7:TR?=	NEC1Opk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
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BB65-RCC	MkLDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			Moj6TWM2OD1\|Lkm3OVQ4KM7:TR?=	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
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MN-60	NY\6RmlnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NXnhZYozUUN3ME20MlExQDdizszN	MoDlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
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no-11	Ml;2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NWG0b5F1UUN3ME21Mlc3OzR\|IN88US=>	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
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HCT116	MYTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?		NIjCSmg1QCCqcoO=	NHjpb2xCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG\|c3H5MEBKSzVyIE2gNE4yKM7:TT6=	MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjdyNUCyNkc,OjJ5MEWwNlI9N2F-
A549	M4C4PWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M4\yXlQ5KGi{cx?=	NHLMe3dCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFG1OFkh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuNUDPxE1w	NIe3e2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkewOVAzOid-MkK3NFUxOjJ:L3G+
HEK293	M4XYfmZ2dmO2aX;uJIF{e2G7			NHy4ZYhKdmirYnn0bY9vKG:oIHj1cYFvKENvdHXycYlv[WxiRlzBS{11[WepZXSgTGRCSzFiaX6gTGVMOjl\|IHPlcIx{NCCLQ{WwJF0hOC5zMjFOwG0v	NFW1[3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS0NVg1Pid-MUm0OFE5PDZ:L3G+
HEK293	MonxSpVv[3Srb36gZZN{[Xl?			M3LWWWlvcGmkaYTpc44hd2ZiSFTBR\|EhcW5iSFXLNlk{KGOnbHzzMEBKSzVyIE2gNE4yOiEQvF2u	M3vI[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{C4OVY{Lz5zOEOwPFU3OzxxYU6=
SNU16	M1fRS2Z2dmO2aX;uJIF{e2G7			M{L0d2lvcGmkaYTpc44hd2ZiSFTBR{Bnem:vIHj1cYFvKFOQVUG2JINmdGy\|LDDJR\|UxKD1iMD6yN\|Mh\|ryPLh?=	NIDiVGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
HEK293	M2HPdGZ2dmO2aX;uJIF{e2G7			NIqwTmZKdmirYnn0bY9vKG:oIHj1cYFvKENvdHXycYlv[WxiRlzBS{11[WepZXSgTGRCSzJiaX6gTGVMOjl\|IHPlcIx{NCCLQ{WwJF0hOC5{NTFOwG0v	MmGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2NEG4OFYoRjF7NESxPFQ3RC:jPh?=
U937	MVfGeY5kfGmxbjDhd5NigQ>?			MU\Jcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNzIHnuJHU6OzdiY3XscJMh[nliaX3teY5weHKnY3nwbZRifGmxbjDhd5NigSxiSVO1NEA:KDBwMzFOwG0v	MmKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OEGyN\|goRjF6M{ixNlM5RC:jPh?=
SK-N-BE(2)	M1XUPWFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M1nGd\|Q5KGi{cx?=	NH;YfnhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLMW4uSkVqMjmgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwM{Og{txONg>?	M3\uUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NUGxPVA3Lz5{OEWxNVkxPjxxYU6=
AGS	NETPVYVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NUPm[ZNZPzJiaILz	NWLON4F6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDBS3Mh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuN\|Qh\|ryPLh?=	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd6N{K2Nkc,Ojl5OEeyOlI9N2F-
MCF7	M{KzVmFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M2njNlQ5KGi{cx?=	NH3o[WlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgeJJm[XSnZDDmc5IhPDhiaILzJIZwdGyxd3XkJIJ6KHKnZoLld4hm\CCndnXyfUA6PiCqcoOgcYVie3W{ZXSgc44h\GG7IEGwJIJ6KEyxd4L5JIF{e2G7LDDJR\|UxKD1iMD6zOUDPxE1w	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjZzM{[zOUc,OjZ4MUO2N\|U9N2F-
HEK293	Mlq0SpVv[3Srb36gZZN{[Xl?			MUHJcohq[mm2aX;uJI9nKGi3bXHuJGMufGW{bXnuZYwhTkyDRz30ZYdo\WRiSFTBR\|MhcW5iSFXLNlk{KGOnbHzzMEBKSzVyIE2gNE41KM7:TT6=	NVvmZnZbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OFE5PDZpPkG5OFQyQDR4PD;hQi=>
HEK293	NHT0R4NHfW6ldHnvckBie3OjeR?=			NXzORlRsUW6qaXLpeIlwdiCxZjDISGFEOyCrbjDISWszQTNiY3XscJMtKEmFNUCgQUAxNjRizszNMi=>	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDNyOEW2N{c,OTh\|MEi1OlM9N2F-
HEL	M375NGZ2dmO2aX;uJIF{e2G7		NHG3N5k{KGi{cx?=	MXjJcohq[mm2aX;uJI9nKGOuYYPzJFEhUESDQzDpckBJTUxiY3XscJMhfXOrbnegRo9kNUy7czCoZYNmfHmuKT3BUWMh[XNic4Xid5Rz[XSnIIDy[Ylv[3WkYYTl[EBnd3JiMzDodpMh\m:ubH;3[YQh[nlic4Xid5Rz[XSnIHHk[Il1cW:wIH3lZZN2emWmIHHmeIVzKDNiaILzJIJ6KG[udX;y[ZNk\W6lZTDhd5NigSxiSVO1NEA:KDBwNEOg{txONg>?	MnzLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3MUG5NFYoRjJ6NUGxPVA3RC:jPh?=
PC3	NWi4OlhkT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NU\aW4IxPDhiaILz	MV\Hdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDQR\|Mh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IIP1cIZwemixZHHtbY5mKEJiYYPzZZktKEeLNUCgQUAxNjR\|IN88UU4>	MkDwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh\|OUWxOVAoRjJ6M{m1NVUxRC:jPh?=
HEL	NGLRZWxCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		NXvwXYpwPzJiaILz	NGXUfVFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjFUEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMD60OEDPxE1w	NEjHOYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe4O\|I3Oid-Mkm3PFczPjJ:L3G+
MOLT4	M2DrWGFvfGmycn;sbYZmemG2aY\lJIF{e2G7		MkfCO\|IhcHK\|	MkLSRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPT1zUOEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMD60OUDPxE1w	NULxbnNoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3PFczPjJpPkK5O\|g4OjZ{PD;hQi=>
HCT116	NW\CWG52SW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NEnQbpI4OiCqcoO=	MofmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCKQ2SxNVYh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IFPDT{05KGG\|c3H5MEBKSzVyIE2gNE41PyEQvF2u	M1joPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7MkCyN\|k4Lz5{OUKwNlM6PzxxYU6=
HEL	MoDkRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?		M1rZPVQ5KGi{cx?=	MVLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFjFUEBk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR\|UxKD1iMD60PEDPxE1w	Ml2xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh3MUG5NFYoRjJ6NUGxPVA3RC:jPh?=
HCT116	NHvQUJhHfW6ldHnvckBie3OjeR?=			NWP1[ZdEUW6qaXLpeIlwdiCxZjDoeY1idiCKQ2SxNVYh[2WubIOsJGlEPTBiPTCwMlUh\|ryPLh?=	M1LFdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6MkS3OVU1Lz5zOEK0O\|U2PDxxYU6=
U937	M2LDVmFvfGmycn;sbYZmemG2aY\lJIF{e2G7		NVfZNW9RPzJiaILz	MmDkRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCXOUO3JINmdGy\|IHnuZ5Vj[XSnZDDmc5IhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NEA:KDBwNUWg{txONg>?	NVvrOVh3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OVM4OjJpPkK1PVU{PzJ{PD;hQi=>
U937	M{fYbmFvfGmycn;sbYZmemG2aY\lJIF{e2G7		M{jURlczKGi{cx?=	MknrRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCXOUO3JINmdGy\|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiY3XscEBoem:5dHigZYZ1\XJiN{KgbJJ{KGK7IF3UWE1j[XOnZDDhd5NigSxiSVO1NEA:KDBwNUWg{txONg>?	NV7JOm1IRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4O\|Q{OjZpPkK1PFc1OzJ4PD;hQi=>
HCT116	NUj3PY0xT3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=		NXy5UVU6OiCmYYnz	NEj4TYlIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhOiCmYYnzJIJ6KE2WVDDhd5NigSxiR1m1NEA:KDBwNjFOwG0v	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjV2MUO5OEc,OjJ3NEGzPVQ9N2F-
HL60	MUHGeY5kfGmxbjDhd5NigQ>?		MlPCN{BpenN?	NHjEe3BKdmirYnn0bY9vKG:oIHPsZZN{KDFiSFTBR{BqdiCqdX3hckBJVDZyIHPlcIx{KHW\|aX7nJGJw[y2DYz3MfZMuSU2FIHHzJJN2[nO2cnH0[UBqdmO3YnH0[YQh\m:{IEOgbJJ{KG[xbHzve4VlKGK7IIP1ZpN1emG2ZTDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiB\|IHjyd{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVAhRSByLk[yJO69VS5?	M{DQPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEG1NFA6Lz5{OESxOVAxQTxxYU6=
MOLT4	NGLBW4NCdnSrcILvcIln\XKjdHn2[UBie3OjeR?=		M1vJdVQ5KGi{cx?=	M2XYSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTV;MWFQh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OCB;IECuOlUh\|ryPLh?=	NY[0OYpYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki1NVE6ODZpPkK4OVEyQTB4PD;hQi=>
HCT116	MlLwRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?			NHu2bXFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{whUUN3MDC9JFAvPjdizszNMi=>	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTd2MkS5Okc,OjF5NEK0PVY9N2F-
HCT116	MVXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>?			M4T4UGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIF{e2W\|c3XkJIF{KGe{b4f0bEBqdmirYnn0bY9vNCCLQ{WwJF0hOC54NzFOwG0v	Ml:2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4NUCyNlEoRjJzNkWwNlIyRC:jPh?=
HCT116	NXLqc45ISW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NVvZWY12PzJiaILz	NFiwUGRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGC1N{BmgHC{ZYPzbY5oKEiFVEGxOkBk\WyuczDh[pRmeiB5MjDodpMh[nliY3XscJRqfGW{LXLseYUh[2WubDD2bYFjcWyrdImgZZN{[XluIFPDOVAhRSByLkeg{txONg>?	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR2MUi0Okc,OTl2NEG4OFY9N2F-
HCT116	Mn;oRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl?			M4WwVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSFPUNVE3KGOnbHzzJIJ6KEOnbHzUbZRmei2EbIXlJINmdGxidnnhZoltcXS7IHHzd4F6NCCLQ{WwJF0hOC55IN88UU4>	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN5MEO3N{c,OTh\|N{CzO\|M9N2F-
HEK293T	M1joXWZ2dmO2aX;uJIF{e2G7		MlXMNUBpeg>?	NX\0b2IxUW6qaXLpeIlwdiCxZjDGUGFINXSjZ3fl[EBJTEGFMjCoeY5sdm:5bjDvdolocW5rIHX4dJJme3OnZDDpckBJTUt{OUPUJINmdGy\|IIXzbY5oKGGlZYT5cIF1\WRibInzbY5mKHOrZHWgZ4hicW5iYYOgd5Vje3S{YYTlJIFnfGW{IEGgbJIh[nliY3;sc5JqdWW2cnnjJIRmfGWldHnvckBj[XOnZDDhd5NigSxiSVO1NEA:KDBwN{Sg{txONg>?	MmDYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{OEm4PFUoRjJ7Mki5PFg2RC:jPh?=
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HCT116	NY\sdY5lSW62aYDyc4xq\mW{YYTpeoUh[XO\|YYm=		NWjr[ZJbOyCmYYnz	MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczDh[pRmeiB\|IHThfZMh[nliV2PUMVEh[XO\|YYmsJGlEPTBiPTCwMlc3KM7:TT6=	MkLnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ\|MkGyNVUoRjJ{M{KxNlE2RC:jPh?=
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U937	NHHyOGNHfW6ldHnvckBie3OjeR?=			NV20TYRiUW6qaXLpeIlwdiCxZjDoeY1idiCKRFHDOEBqdiCXOUO3JINmdGy\|IHL5JIludXWwb4Dy[YNqeGm2YYTpc44h[XO\|YYmu	NUjaZ3ZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPFEzOzhpPkG4N\|gyOjN6PD;hQi=>
U937	MW\GeY5kfGmxbjDhd5NigQ>?			MXrJcoR2[3Srb36gc4Yh\3KjboXsc4N6fGmlIHTp[oZmemWwdHnheIlwdiCrbjDoeY1idiCXOUO3JINmdGy\|Lh?=	NFL3fHI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO4NVI{QCd-MUizPFEzOzh:L3G+
HCT116	MmfLSpVv[3Srb36gZZN{[Xl?	MlniNVAhfU1?	MnnoNlQhcHK\|	NWrmd4llUW6qaXLpeIlwdiCxZjDISGFEOSCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO\|ZXSgZZMhcW6mdXP0bY9vKG:oIHj5dIVz[WOndInsZZRqd25ib3[gbIl{fG:wZTDIN{BifCBzMDD1UUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{Ng>?	M1fGR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWyNlI3Lz5{MES1NlIzPjxxYU6=
NB4	M3rxNGNmdGxiY4njcIUh[XK{ZYP0JIF{e2G7	M13Pe\|UhfU1?	NUPWZ4E6OjRiaILz	NX[zSFBbS2WubDDjfYNt\SCjcoLld5QhcW5iaIXtZY4hVkJ2IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJIFk[3WvdXzheIlwdiCjdDDwdoUuTzFicHjhd4Uh[XRiNTD1UUBi\nSncjCyOEBpenNiYomgSmFEWyCjbnHsfZNqey5?	MmDZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2OUG0OFAoRjJyNEmxOFQxRC:jPh?=
NB4	MkPZSpVv[3Srb36gZZN{[Xl?	NWTFfnNVPSC3TR?=	M{nueFI1KGi{cx?=	NILDWm9KdmS3Y4Tpc44hd2ZicEKxW2FHOS:FSWCxJIV5eHKnc4Ppc44hcW5iaIXtZY4hVkJ2IHPlcIx{KGG2IEWgeW0h[W[2ZYKgNlQhcHK\|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqey5?	NVmyblRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0PVE1PDBpPkKwOFkyPDRyPD;hQi=>
epithelial cells	NH2yXZNHfW6ldHnvckBie3OjeR?=	Mo\WNUB2VQ>?	MUK0MEA3NCCjbnSgPEBl[Xm\|	M37ncmlvcGmkaYTpc44hd2ZiSFTBR\|chcW5iaIXtZY4hcG:vb4r5[492eyCIZHXseIE2ODhicILpcYFzgSCkcn;uZ4hq[WxiZYDpeIhmdGmjbDDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckB{cG:{dDDjbZJkfWm2IHP1dpJmdnS\|IHH0JFEhfU1iZn;yJFQtKDZuIHHu[EA5KGSjeYOgdoVt[XSrdnWgeI8h[2:wdILvcE4>	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4Nke4PUc,OTl7Nk[3PFk9N2F-
Hela	MY\GeY5kfGmxbjDhd5NigQ>?	NF\2[mgxNjZidV2=	M3\5VVE3KGi{cx?=	M3fBfmlvcGmkaYTpc44hd2ZiSFTBR\|YhcW5iaIXtZY4hUGWuYTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckB1d3SjbDDsfZNqdmViYXPleJlt[XSrb36gZZQhOC54IIXNJIFnfGW{IEG2JIhzeyCkeTDpcY12dm:obIXvdoV{[2WwY3WgcYlkem:\|Y3;wfU4>	NIK1c\|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[5Olk2Pid-MkG2PVY6PTZ:L3G+
U937	MkS5R4VtdCCleXPs[UBienKnc4SgZZN{[Xl?	NG\JT4k2KHWP	NFHmS\|c{OCCqcoO=	NIS3eZJE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBWQTN5IHPlcIx{KGG\|c3Xzd4VlKGG\|IHHjZ5VufWyjdHnvckBifCCJMTDwbIF{\SCjdDC1JJVOKGGodHXyJFMxKGi{czDifUBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nJIJie2WmIH\sc5ch[3m2b33leJJ6Ng>?	MoPzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{MUW2OFcoRjJzMkG1OlQ4RC:jPh?=
epithelial cells	MYHGeY5kfGmxbjDhd5NigQ>?	MYS1JJVO		MlT6TY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJJBzcW2jcomgZpJwdmOqaXHsJIVxcXSqZXzpZYwh[2WubIOgZZN{\XO\|ZXSgZZMhcW6mdXP0bY9vKG:oIH31eIFvfCCIZHXseIE2ODhiQ1\UVkBxem:2ZXnuJIFxcWOjbDDzeZJn[WOnIHzvZ4FtcXqjdHnvckBifCB3IIXNMi=>	NFXkbmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2Olc5QSd-MUm5OlY4QDl:L3G+
NB4	MnPCSpVv[3Srb36gZZN{[Xl?	M1HhOlUhfU1?	NF7tSmgzPCCqcoO=	NHO3OZJKdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hVkJ2IHPlcIx{KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFMh[WOndInsZZRqd25iYYSgOUB2VSCjZoTldkAzPCCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|Lh?=	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR7MUS0NEc,OjB2OUG0OFA9N2F-
C127-LT	Mom0SpVv[3Srb36gZZN{[Xl?	NHXxUocyKHWP	MWSyOEBpenN?	NX;RWFM6UW6qaXLpeIlwdiCxZjDISGFEKGmwIH3veZNmKENzMketUHQh[2WubIOgZZN{\XO\|ZXSgZZMh[WO2aY\heIlwdiCxZjDFS2ZRKGW6cILld5Nqd25iYYSgNUB2VSCjZoTldkAzPCCqcoOgZpkh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcImu	Mo\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ7OU[zO\|IoRjJ4OUm2N\|czRC:jPh?=
C127-LT	Mn\ISpVv[3Srb36gZZN{[Xl?	NIDnSYcyOCC3TR?=	NYG1dXN5OjRiaILz	M2fUcWlvcGmkaYTpc44hd2ZiSFTBR{BqdiCvb4Xz[UBEOTJ5LVzUJINmdGy\|IHHzd4V{e2WmIHHzJIFkfGm4YYTpc44hd2ZiRVfGVEBmgHC{ZYPzbY9vKGG2IEGwJJVOKGGodHXyJFI1KGi{czDifUBndHWxcnXzZ4Vv[2VibXnjdo9{[2:yeT6=	M3TlTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4OUm2N\|czLz5{Nkm5OlM4OjxxYU6=
HCT116	NVjyd4ZJTnWwY4Tpc44h[XO\|YYm=	MXuxNEB2VQ>?	NH[3b2EzPCCqcoO=	MWXJcohq[mm2aX;uJI9nKEiGQVOyJIlvKGi3bXHuJGhEXDFzNjDj[YxteyCjc4Pld5Nm\CCjczDofZBmemGlZYT5cIF1cW:wIH;mJIhqe3SxbnWgTFMh[XRiMUCgeW0h[W[2ZYKgNlQhcHK\|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqey5?	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MUKxOUc,OjJ\|MkGyNVU9N2F-
HCT116	NGroemFHfW6ldHnvckBie3OjeR?=	NV;N[ZNwOTBidV2=	NFLMRmozPCCqcoO=	NW\4WJhXUW6qaXLpeIlwdiCxZjDISGFEOSCrbjDoeY1idiCKQ2SxNVYh[2WubIOgZZN{\XO\|ZXSgZZMhcHmyZYLhZ4V1gWyjdHnvckBw\iCqaYP0c45mKEh\|IHH0JFExKHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYOu	M2DrV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{M{KxNlE2Lz5{MkOyNVIyPTxxYU6=
TC32	NXrDcWxIeUiWUzDhd5NigQ>?			MVvxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhXEN\|MjDj[Yxtey5?	NIfLd2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
U-2 OS	M3:yUpFJXFNiYYPzZZk>			M3z1S5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCXLUKgU3Mh[2WubIOu	M1i4[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
A673	NVXKPVJKeUiWUzDhd5NigQ>?			NH75eohyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucz6=	NXvMXmJTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
DAOY	NXzhc4FXeUiWUzDhd5NigQ>?			NV;2OlBMeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IFTBU3kh[2WubIOu	NYnhbodMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2	M{DHdJFJXFNiYYPzZZk>			MVrxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy\|Lh?=	MoThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-37	NIm5NXhyUFSVIHHzd4F6			NIPvN25yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStN\|ch[2WubIOu	M1vBelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD	MlPwdWhVWyCjc4PhfS=>			NFXj[YVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUlSgZ4VtdHNw	M1m4NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-SH	NETnboZyUFSVIHHzd4F6			M1fkXZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMXNJKGOnbHzzMi=>	MoP4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-12	NXW3bIt3eUiWUzDhd5NigQ>?			NHGzSGxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStNVIh[2WubIOu	MljMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R)	MoDOdWhVWyCjc4PhfS=>			MmTDdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy\|Lh?=	M17NdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643	Mnm1dWhVWyCjc4PhfS=>			NWnGZYZoeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsd{4>	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
OHS-50	MVrxTHRUKGG\|c3H5			NFq4c2xyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzzMi=>	NWKzRVNsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
fibroblast cells	M3LTT5FJXFNiYYPzZZk>			M3XiXpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiClb370do9tKEiqIIfpcIQhfHmyZTDmbYJzd2KuYYP0JINmdGy\|Lh?=	NFO3SY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
LAN-5	M3LPWpFJXFNiYYPzZZk>			NF\wRoZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBNSU5vNTDj[Yxtey5?	M3XqZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
DAOY	MVfxTHRUKGG\|c3H5			MnXSdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgSGFQYSClZXzsd{4>	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
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Jurkat	M1fXO2NmdGxiY4njcIUh[XK{ZYP0JIF{e2G7	MUS1JJVO	NIPCfHEzPCCqcoO=	MlrBR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gTpVzc2G2IHPlcIx{KGG2IEWgeW0h[W[2ZYKgNlQhcHK\|IHL5JJBzd3CrZHn1cUBqd2SrZHWgd5RicW6rbnetZoF{\WRiZnzve{BkgXSxbXX0dolkKG2ndHjv[E4>	NWG0dXZWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1PFk1PDFpPkK5OVg6PDRzPD;hQi=>
MV4-11	NF7lR4VHfW6ldHnvckBie3OjeR?=	NYXxPZJzPSC3TR?=	NV[wUYxGOjRiaILz	MV;Jcohq[mm2aX;uJI9nKGOuYYPzJFEhUESDQzDpckBpfW2jbjDNWlQuOTFiY3XscJMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIhqe3SxbnWgbJlx\XKjY3X0fYxifGmxbjDheEA2KHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgcYV1cG:mLh?=	M{n5TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NUi5OFQyLz5{OUW4PVQ1OTxxYU6=
LNCAP	M{nBXmZ2dmO2aX;uJIF{e2G7	NG\oWmMyOCC3TR?=	M1n2dVI1KGi{cx?=	NYDkVXpqUW6qaXLpeIlwdiCxZjDISGFEPiCrbjDoeY1idiCOTlPBVEBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFTIWE1qdmS3Y3XkJIFteGijLYT1ZpVtcW5iZHXhZ4V1gWyjdHnvckBjgSCvZXHzeZJqdmdiaX7jdoVie2ViaX6gZYxxcGFvdIXieYxqdiCjY3X0fYxifGmxbjDheEAyOCC3TTDt[YF{fXKnZDDh[pRmeiB{NDDodpMhemWuYYTpeoUhfG9iY3;ueJJwdC5?	NFnvW3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{exO\|U1PCd-Mke3NVc2PDR:L3G+

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Assay

Methods	Test Index	PMID
Western blot	AcH3 / AcH4 / HDAC1 / HDAC2 / HDAC3; PubMed: 24203492 Pharm Res HCT-15 or fresh primary NK cells were treated with entinostat for 24 h, and AcH3, AcH4, and HDAC1, 2, and 3 were detected by Western blotting. Cleaved PARP; PubMed: 19148581 J Neurooncol Induction of PARP cleavage in Daoy cells upon exposure to MS-275 was demonstrated by Western blot analyses using antibodies that recognize the cleaved 89 kDa PARP protein. Pan-Actin (45 kDa) was used as a loading control. Caspase 8 ; PubMed: 19148581 J Neurooncol Cleavage of pro-Caspase-8 was demonstrated by Western blotting with extracts prepared from Daoy cells treated with 2.5 µM MS-275 for various time periods. Antibodies specific to human Caspase-8 or the loading control, pan-Actin were used to probe protein expression. DR4 / DR5; PubMed: 19148581 J Neurooncol Western blotting to detect DR4 and DR5 expression upon treatment of Daoy cells with 2.5 µM MS-275 for 4, 8 and 24 h. TopoIIα / TopoIIβ; PubMed: 21254166 Hepatology Upper panel, concentration-dependent effects of AR42, MS-275 and vorinostat on the expression of topoIIα versus topoIIβ after 48 h. Lower panel, time-dependent effect of 0.5 μM AR42 and 5 μM MS-275 on topoIIα versus topoIIβ expression in PLC-5 cells.	24203492 19148581 21254166
Immunofluorescence	phalloidin / snail; PubMed: 29855565 Sci Rep (C) Effect of MS-275 treatment on Snail expression and localization. MCs (mesothelial cells) were treated with MS-275 (250 nM), or DMSO for 48 hr and then stimulated with TGFβ1 (2 ng/ml) for 24 h before processing for immunofluorescence with a monoclonal antibody against Snail. Cell nuclei are shown in blue (Hoechst 33342). Confocal images are shown from one representative experiment of three performed. DR4 / DR5; PubMed: 19148581 J Neurooncol Flow Cytometry to measure cell surface expression of FAS, DR4 and DR5 using PE-labeled antibodies. Cells stained with isotype matched anti-human IgG antibodies served as controls. H3K4Me3 / H3K9Me3; PubMed: 22468166 Mol Cell Pharmacol Immunocytochemical analysis of the effects of AR42, SAHA, and MS-275 at the indicated concentrations on H3K4Me3 (upper panels) and H3K9Me3 (lower panels) after 24 h of treatment in LNCaP cells.	29855565 19148581 22468166
Growth inhibition assay	Cell proliferation; PubMed: 24203492 Pharm Res (A) HCT-15 cells were cultured in indicated concentrations of entinostat for up to 72 h, and proliferation determined by MTT assay as indicated. (B) HCT-15, CCH-OS-D, COL and HOS cells were cultured in indicated concentrations of entinostat for 72 h, and viable cell number was determined using the Vi-cell assay and proliferation index calculated. (C) Freshly-isolated human NK cells were cultured in indicated concentrations of entinostat for up to 72 h, and viability was assessed by flow cytometry for 7-AAD uptake. , P < 0.05, ; P ≤ 0.01; **, P ≤ 0.001 Cell viability; PubMed: 28969017 Oncotarget PANC-1, MIA PaCa-2, BxPC3, CFPAC-1, SUIT2 and SUIT2 Clone 1 cells were plated at a density of 3-5×103 per well in 96-well microtiter plates, allowed to adhere overnight and incubated for 72 hours in the presence of variable concentrations of entinostat (0-100μM). Cell viability was determined by XTT assays. The data presented are the mean values from triplicate wells from two independent experiments ±SE.	24203492 28969017
ELISA

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