Entinostat (MS-275)

For research use only. Not for use in humans.

目录号：S1053 别名： SNDX-275 中文名称：恩替诺特

CAS No. 209783-80-2

Entinostat (MS-275, SNDX-275)强烈抑制HDAC1和HDAC3，无细胞试验中IC50分别为0.51 μM和1.7 μM，抑制作用强于HDACs 4， 6， 8，和10。Entinostat可诱导自噬和凋亡。Phase 3。

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价格

库存

购买数量

10mM (1mL in DMSO)	RMB 746.45	现货
10mg	RMB 794.43	现货
25mg	RMB 1233.32	现货
50mg	RMB 2234.07	现货
200mg	RMB 6301.79	现货
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客户使用Selleck生产的Entinostat (MS-275)发表文献312篇：

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产品安全说明书

HDAC抑制剂选择性比较

生物活性

产品描述

靶点

HDAC1 ^[2] (Cell-free assay)	HDAC3 ^[2] (Cell-free assay)
0.51 μM	1.7 μM

体外研究

MS-275通过作用于2′-氨基而抑制HDACs。MS-275作用于 K562细胞，诱导 p21^WAF1/CIP1和凝溶胶蛋白的累积。MS-275作用于A2780细胞，可以降低S期细胞，提高G1期细胞。 MS-275通过抑制HAD而抑制人类肿瘤细胞系，包括 A2780, Calu-3, HL-60, K562, St-4, HT-29, KB-3-1, Capan-1, 4-1St和 HCT-15细胞增殖， IC50 为41.5 nM到4.71 μM。^[1]MS-275抑制HDACs，作用于HDAC1和 HDAC3时IC50 分别为0.51 μM和1.7 μM。而对其他的HDACs没有抑制效果，如HDAC4, 6, 8和10。^[2]MS-275有效抑制人类白血病和淋巴癌细胞，包括U937, HL-60, K562, 和Jurkat。MS-275可以诱导U937细胞p21CIP1/WAF1 调节的生长和变异Marker (CD11b)的表达。MS-275降低cyclin D1和抗凋亡蛋白Mcl-1与XIAP的表达。^[3]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
SCC-3	Mn7SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NV65XWFIUUN3ME2wMlA3OSEQvF2=	M{j3OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
ALL-PO	M1HwVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NHe3UWxKSzVyPUCuNFY{PTVizszN	NIny[HM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
697	M1X1Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NF\QToVKSzVyPUCuNFk6PzZizszN	NEDSeG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
NCI-H748	MlPsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M{WwdWlEPTB;MD6xNFM{PCEQvF2=	M2T1R\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
NKM-1	MnrRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MWfJR\|UxRTBwMUC5NVIh\|ryP	NHXtUoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
ES1	M3TqXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NYXGcm9uUUN3ME2wMlEyOjV3IN88US=>	M1LXV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
NCI-H1963	MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MoLCTWM2OD1yLkGxOVc6KM7:TR?=	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
NCI-H1417	M{[zWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NHqwbmlKSzVyPUCuNVI6PzRizszN	NIG0N249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
NEC8	M1fubWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MmX5TWM2OD1yLkGzOVI4KM7:TR?=	M{n4TlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
CRO-AP2	NV[wTGVET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MmjBTWM2OD1yLkG2PFg6KM7:TR?=	NF;TdZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
A3-KAW	MlrvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NIrPeWpKSzVyPUCuNVc3OjdizszN	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
SF539	M4fafGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NWLhSlBnUUN3ME2wMlE6PTl\|IN88US=>	MkTRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
NOS-1	MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NH\LNFhKSzVyPUCuNVk3OTlizszN	NXXwPJlQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
NTERA-S-cl-D1	M{L0cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			MoXWTWM2OD1yLkKwNVE{KM7:TR?=	NHnVW3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
COR-L88	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MWDJR\|UxRTBwMkK5OVkh\|ryP	NGf2dJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
EM-2	MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M2XHOWlEPTB;MD6yOFA4QSEQvF2=	Ml73QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
KARPAS-45	NEPieY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M1vqVGlEPTB;MD6yO\|g{OyEQvF2=	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
DSH1	M{XsRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NU\a[3VvUUN3ME2wMlI5PzB6IN88US=>	M{nG[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
HT-144	NHOw[GNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NFrIUJNKSzVyPUCuN\|AzPTZizszN	M3:5NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
ATN-1	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NUT5XYlmUUN3ME2wMlMxPTd4IN88US=>	Mn3YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
HEL	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NUDLeFI2UUN3ME2wMlMyOzR6IN88US=>	M2XIdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
NB12	NUnrZohNT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MkjtTWM2OD1yLkOxO\|U3KM7:TR?=	NU[yOlRNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
LU-139	NWXlV\|FmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NIHvN5hKSzVyPUCuN\|M2OSEQvF2=	NXHifnkxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
J-RT3-T3-5	M3v5fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M3:3Z2lEPTB;MD6zN\|cyPiEQvF2=	M1z4UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
MOLT-13	MkDwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MXvJR\|UxRTBwM{O4NUDPxE1?	NUGyfYFxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
SR	NVPTXJB{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NEX0PHpKSzVyPUCuN\|QzPjFizszN	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
CMK	NF21V2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M3;jVGlEPTB;MD6zOVczPyEQvF2=	NHPCdpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
ES8	NWLZU5p3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MmHETWM2OD1yLkO2NFIzKM7:TR?=	MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
LB647-SCLC	NIHmRZlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NFq4coxKSzVyPUCuN\|Y4OyEQvF2=	M1nhWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
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HCC2218	MlnyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NUPMTmFlUUN3ME2xMlE3PjRzIN88US=>	NIrBe\|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
HAL-01	M4PMeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M3\NS2lEPTB;MT6xOlk1OyEQvF2=	M1P5clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
IST-MEL1	NUHC[mlXT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXPJR\|UxRTFwMUe2OVkh\|ryP	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
NCI-H719	NVfGRpdOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M13iZ2lEPTB;MT6xO\|g6QCEQvF2=	NGnoZ5Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
EVSA-T	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NGTXXo9KSzVyPUGuNVgyOTRizszN	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
SK-NEP-1	NV3oOpVuT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M3yzNWlEPTB;MT6yNFI3PiEQvF2=	NVO4R5RXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
OCUB-M	NHuwVJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MUDJR\|UxRTFwMkG0PFkh\|ryP	NEjDXJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
MEG-01	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MnHLTWM2OD1zLkKyNVE5KM7:TR?=	NGXCfYc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
no-10	M4\JS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NX7mOG9wUUN3ME2xMlI{OTF{IN88US=>	MnrsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
MHH-CALL-2	NWTKXnBmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MmjHTWM2OD1zLkK0O\|IyKM7:TR?=	M{X0PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
SK-N-DZ	MmD1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NUHaOWRsUUN3ME2xMlI1Pzd4IN88US=>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
SCLC-21H	MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M4PrSGlEPTB;MT6yOlQ4QCEQvF2=	NYjPNnBLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
CTV-1	M2G0TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NVPNcVk5UUN3ME2xMlI4PDJ3IN88US=>	NILJ[og9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
NB1	NWnmTG1GT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NWPvRpE5UUN3ME2xMlI4PzN{IN88US=>	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
NCI-H64	MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MmXwTWM2OD1zLkK4OFYzKM7:TR?=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
MDA-MB-134-VI	MlP3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MWfJR\|UxRTFwMki1O\|ch\|ryP	NXvXcHJ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
LB2241-RCC	NVT6NWYyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MofjTWM2OD1zLkK4OlY{KM7:TR?=	NFTLU3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
8-MG-BA	MmXkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NGLnWXBKSzVyPUGuNlg5PjZizszN	NVG5dZRMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
LP-1	Mlu5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NFfFSW5KSzVyPUGuNlk6PDdizszN	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
LS-411N	MnHsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MVfJR\|UxRTFwM{C5PVgh\|ryP	MmrKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
CAL-148	NYjFWYZ5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Mn;2TWM2OD1zLkOyOVQzKM7:TR?=	NV33VVh2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
NCI-H2171	MoP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MV3JR\|UxRTFwM{S1NFIh\|ryP	MoXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
JiyoyeP-2003	M4rqNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NGL1VY9KSzVyPUGuN\|U{QSEQvF2=	NHTzO\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
NCI-H2107	NGTlR4FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NESxTHBKSzVyPUGuN\|U5QDNizszN	M4rZXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
BB30-HNC	MoLMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M4KxWWlEPTB;MT6zPFk4QCEQvF2=	MkmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
K-562	MkPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			NIjNZ\|JKSzVyPUGuN\|kzOTlizszN	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
PSN1	NVrYSI5HT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MX;JR\|UxRTFwNEKyPFch\|ryP	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
HCC2157	NXn4cm9{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXvJR\|UxRTFwNEK2PVEh\|ryP	NYfoWJpVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
SBC-1	MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NULae2oyUUN3ME2xMlQzPzRzIN88US=>	NIDVNHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
MC116	NVrEZZdNT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXPJR\|UxRTFwNEO2NVUh\|ryP	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
KARPAS-422	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NFzESGRKSzVyPUGuOFU{PThizszN	Mk\MQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
LB996-RCC	NI\sRZhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NHP2UphKSzVyPUGuOFcyODNizszN	NG\SW5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
MSTO-211H	NH31TWRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MnnFTWM2OD1zLkS3PVg4KM7:TR?=	M3XV[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
BT-474	MojtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MU\JR\|UxRTFwNUG3OlQh\|ryP	M3PHU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
A388	NYS5NmliT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M1i1fWlEPTB;MT61NVk1PSEQvF2=	NHXt[FY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
SJSA-1	NUXoXVVOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NHTnbllKSzVyPUGuOVIzPiEQvF2=	M2nGSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
COLO-829	NXrvVXZMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Mm\1TWM2OD1zLkWzOVY1KM7:TR?=	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
KM-H2	MmTvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M1TKbWlEPTB;MT61OlY4KM7:TR?=	MnTEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
GR-ST	MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MYjJR\|UxRTFwNU[4NkDPxE1?	NVrCcINkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
RPMI-8866	NXzLTVJMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MnXpTWM2OD1zLk[wNVQ1KM7:TR?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
KG-1	MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			M3:yUmlEPTB;MT62NVkxOSEQvF2=	M{jG[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
NCI-H82	NVvwOGlnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			M4rubWlEPTB;MT62N\|QxPiEQvF2=	NWTqdHZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
LB1047-RCC	NVP6WlM4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MoTITWM2OD1zLk[zOFU6KM7:TR?=	NVXDZ3hRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
KM12	NX33XHloT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NVLVVnVsUUN3ME2xMlY1PyEQvF2=	M1XyUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
NB5	M1v4Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			Ml;MTWM2OD1zLk[1Olc4KM7:TR?=	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
HDLM-2	M2T1cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NHruWYFKSzVyPUGuOlgzQDFizszN	M33qRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
KU812	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MnHHTWM2OD1zLk[5OlA2KM7:TR?=	M1WwPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
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OCI-AML2	M{K2dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			NUDROlRKUUN3ME2zMlYzPjdzIN88US=>	NVnkfox5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
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BB65-RCC	NUfSRlc1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			Mn;xTWM2OD1\|Lkm3OVQ4KM7:TR?=	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
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SHP-77	Ml2wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			Mk\MTWM2OD12LkCwOVI1KM7:TR?=	MnLZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlc4PTlxJ{7TRW5ITVJ:L3G+
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LU-65	Mon1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M3zUVmlEPTB;ND6xOVM{OiEQvF2=	NYHn[HN[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
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KINGS-1	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			Mo\WTWM2OD12LkOxOFMzKM7:TR?=	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
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COLO-684	NHjYfmVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MWLJR\|UxRTVwOUm0PVQh\|ryP	NF:3bHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
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EW-24	NHrLXnZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			NEL0cYhKSzVyPU[uNlg2OSEQvF2=	NGLUfGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzPzd3OT:nQnNCVkeHUkyvZV4>
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NCI-H1648	NH\Ud2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MlTTTWM2OD14Lk[3OVU4KM7:TR?=	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyO\|c2QS9pPmPBUmdGWjxxYU6=
LB771-HNC	MonYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			MXPJR\|UxRTZwOUKzNFEh\|ryP	M4TSeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFI4PzV7Lze+V2FPT0WUPD;hQi=>
SK-MEL-1	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			NIT4eW1KSzVyPUiuNVMyPjZizszN	NYW0[4R1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjd5NUmvK\|5USU6JRWK8M4E,
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HEK293	MkLySpVv[3Srb36gZZN{[Xl?			NXH1UIh3UW6qaXLpeIlwdiCxZjDoeY1idiCFLYTldo1qdmGuIF\MRWcufGGpZ3XkJGhFSUN\|IHnuJGhGUzJ7MzDj[YxteyxiSVO1NEA:KDBwNDFOwG0v	NWrKOnJDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0OFE5PDZpPkG5OFQyQDR4PD;hQi=>
HEK293	NHTSc45HfW6ldHnvckBie3OjeR?=			NFLOT3lKdmirYnn0bY9vKG:oIFjERWM{KGmwIFjFT\|I6OyClZXzsd{whUUN3MDC9JFAvPCEQvF2u	MmW3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|MEi1OlMoRjF6M{C4OVY{RC:jPh?=
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NB4	NFXDclVE\WyuIHP5Z4xmKGG{cnXzeEBie3OjeR?=	M4[5ZVUhfU1?	NIK0NmszPCCqcoO=	MW\D[YxtKGO7Y3zlJIFzemW\|dDDpckBpfW2jbjDORlQh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iYXPjeY12dGG2aX;uJIF1KHC{ZT3HNUBxcGG\|ZTDheEA2KHWPIHHmeIVzKDJ2IHjyd{BjgSCIQVPTJIFv[Wy7c3nzMi=>	NYm0S3pjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0PVE1PDBpPkKwOFkyPDRyPD;hQi=>
NB4	Ml3SSpVv[3Srb36gZZN{[Xl?	M{LwZlUhfU1?	NIHQTFMzPCCqcoO=	MmDpTY5lfWO2aX;uJI9nKHB{MWfBSlEwS0mSMTDlfJBz\XO\|aX;uJIlvKGi3bXHuJG5DPCClZXzsd{BifCB3IIXNJIFnfGW{IEK0JIhzeyCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZMv	M37VZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEmxOFQxLz5{MES5NVQ1ODxxYU6=
epithelial cells	M3;4dWZ2dmO2aX;uJIF{e2G7	MUKxJJVO	MVS0MEA3NCCjbnSgPEBl[Xm\|	NWjLO3k5UW6qaXLpeIlwdiCxZjDISGFEPyCrbjDoeY1idiCqb33vfplod3W\|IF\k[Yx1[TVyODDwdolu[XK7IHLyc45kcGmjbDDldIl1cGWuaXHsJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKHOqb4L0JINqemO3aYSgZ5VzemWwdIOgZZQhOSC3TTDmc5IhPCxiNjygZY5lKDhiZHH5d{Bz\WyjdHn2[UB1dyClb370do9tNg>?	M1vyXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OU[2O\|g6Lz5zOUm2Olc5QTxxYU6=
Hela	MVrGeY5kfGmxbjDhd5NigQ>?	MX2wMlYhfU1?	NGDB[VMyPiCqcoO=	NU\EVlVRUW6qaXLpeIlwdiCxZjDISGFEPiCrbjDoeY1idiCKZXzhJINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKHSxdHHsJIx6e2mwZTDhZ4V1gWyjdHnvckBifCByLk[geW0h[W[2ZYKgNVYhcHK\|IHL5JIludXWwb3\seY9z\XOlZX7j[UBucWO{b4Pjc5B6Ng>?	M4jldVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkm2PVU3Lz5{MU[5Olk2PjxxYU6=
U937	NXOw[WRJS2WubDDjfYNt\SCjcoLld5Qh[XO\|YYm=	MUe1JJVO	NWDGfnptOzBiaILz	Ml;QR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gWVk{PyClZXzsd{Bie3Onc4Pl[EBieyCjY3P1cZVt[XSrb36gZZQhTzFicHjhd4Uh[XRiNTD1UUBi\nSncjCzNEBpenNiYomgdJJweGmmaYXtJIlw\GmmZTDzeIFqdmmwZzDiZZNm\CCobH;3JIN6fG:vZYTyfU4>	M1HjW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkG1OlQ4Lz5{MUKxOVY1PzxxYU6=
epithelial cells	M2XoPGZ2dmO2aX;uJIF{e2G7	NWPndIFXPSC3TR?=		Mof2TY5pcWKrdHnvckBw\iCKRFHDJIlvKGi3bXHuJJBzcW2jcomgZpJwdmOqaXHsJIVxcXSqZXzpZYwh[2WubIOgZZN{\XO\|ZXSgZZMhcW6mdXP0bY9vKG:oIH31eIFvfCCIZHXseIE2ODhiQ1\UVkBxem:2ZXnuJIFxcWOjbDDzeZJn[WOnIHzvZ4FtcXqjdHnvckBifCB3IIXNMi=>	MlPyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O\|g6RC:jPh?=
NB4	M3PwR2Z2dmO2aX;uJIF{e2G7	MX:1JJVO	MWWyOEBpenN?	NX;uTXlGUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKE6ENDDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBpcXO2b37lJGg{KGGlZYT5cIF1cW:wIHH0JFUhfU1iYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcz6=	M{jRNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEmxOFQxLz5{MES5NVQ1ODxxYU6=
C127-LT	MXnGeY5kfGmxbjDhd5NigQ>?	Mn;1NUB2VQ>?	MVKyOEBpenN?	NH73SFdKdmirYnn0bY9vKG:oIFjERWMhcW5ibX;1d4UhSzF{Nz3MWEBk\WyuczDhd5Nme3OnZDDhd{Bi[3SrdnH0bY9vKG:oIFXHSnAh\XiycnXzd4lwdiCjdDCxJJVOKGGodHXyJFI1KGi{czDifUBndHWxcnXzZ4Vv[2VibXnjdo9{[2:yeT6=	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjl7NkO3Nkc,OjZ7OU[zO\|I9N2F-
C127-LT	M3nsc2Z2dmO2aX;uJIF{e2G7	MWCxNEB2VQ>?	NVTqOnBJOjRiaILz	MVXJcohq[mm2aX;uJI9nKEiGQVOgbY4hdW:3c3WgR\|EzPy2OVDDj[YxteyCjc4Pld5Nm\CCjczDhZ5RqfmG2aX;uJI9nKEWJRmCg[ZhxemW\|c3nvckBifCBzMDD1UUBi\nSncjCyOEBpenNiYomg[ox2d3Knc3PlcoNmKG2rY4Lvd4NweHlw	NFW4bpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5OlM4Oid-Mk[5PVY{PzJ:L3G+
HCT116	MlnNSpVv[3Srb36gZZN{[Xl?	NX\wcoZQOTBidV2=	NIjX[3czPCCqcoO=	NEXhW41KdmirYnn0bY9vKG:oIFjERWMzKGmwIHj1cYFvKEiFVEGxOkBk\WyuczDhd5Nme3OnZDDhd{BpgXCncnHj[ZR6dGG2aX;uJI9nKGirc4TvcoUhUDNiYYSgNVAhfU1iYX\0[ZIhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcz6=	NViy[po5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzNlEzOTVpPkKyN\|IyOjF3PD;hQi=>
HCT116	NYLQSoV[TnWwY4Tpc44h[XO\|YYm=	M2P0VVExKHWP	NVjESIVqOjRiaILz	M{fjbGlvcGmkaYTpc44hd2ZiSFTBR\|EhcW5iaIXtZY4hUEOWMUG2JINmdGy\|IHHzd4V{e2WmIHHzJIh6eGW{YXPleJlt[XSrb36gc4YhcGm\|dH;u[UBJOyCjdDCxNEB2VSCjZoTldkAzPCCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|Lh?=	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN{MUKxOUc,OjJ\|MkGyNVU9N2F-
TC32	NWDDVFZIeUiWUzDhd5NigQ>?			MV\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhXEN\|MjDj[Yxtey5?	NU\QUXJqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
U-2 OS	Mn\pdWhVWyCjc4PhfS=>			NYDveYI1eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IGWtNkBQWyClZXzsd{4>	NXr6N3hwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
A673	MmPxdWhVWyCjc4PhfS=>			NFznXJpyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucz6=	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
DAOY	Mly0dWhVWyCjc4PhfS=>			M{DkU5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCGQV;ZJINmdGy\|Lh?=	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
Saos-2	MVzxTHRUKGG\|c3H5			NHnyUoNyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU3Hvd{0zKGOnbHzzMi=>	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
BT-37	Mn\XdWhVWyCjc4PhfS=>			NUPxXZE1eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzzMi=>	M2HCOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD	M1jLZZFJXFNiYYPzZZk>			MXPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJMv	M3nvOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-SH	NH;CO3VyUFSVIHHzd4F6			MWfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3TTEBk\Wyucz6=	MkjoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
BT-12	NWjQXWg2eUiWUzDhd5NigQ>?			Mmr6dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKEKWLUGyJINmdGy\|Lh?=	NYLyNnd7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
MG 63 (6-TG R)	MXLxTHRUKGG\|c3H5			MofRdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKE2JIE[zJEg3NVSJIGKpJINmdGy\|Lh?=	MnzmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
NB1643	MVPxTHRUKGG\|c3H5			NYe2S4Q5eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IF7CNVY1OyClZXzsd{4>	MnzvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
OHS-50	NXHGdZBPeUiWUzDhd5NigQ>?			NIjQbYJyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiT1jTMVUxKGOnbHzzMi=>	M3fu[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
fibroblast cells	MmnvdWhVWyCjc4PhfS=>			M3PkdJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiClb370do9tKEiqIIfpcIQhfHmyZTDmbYJzd2KuYYP0JINmdGy\|Lh?=	NGS3OZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
LAN-5	NYfVWFhYeUiWUzDhd5NigQ>?			M3PzTZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJGxCVi13IHPlcIx{Ng>?	MoLHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
DAOY	MWLxTHRUKGG\|c3H5			MUXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDERW9[KGOnbHzzMi=>	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
Rh41	MlHxdWhVWyCjc4PhfS=>			MYfxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtey5?	NXO5OY9ORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N\|UyOzlpPkK5OFM2OTN7PD;hQi=>
NB-EBc1	NVuzNWF7eUiWUzDhd5NigQ>?			MYnxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDORk1GSmNzIHPlcIx{Ng>?	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
Rh30	NF7ufoVyUFSVIHHzd4F6			M2nvNpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEOwJINmdGy\|Lh?=	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
A673	NHv5bYhyUFSVIHHzd4F6			MmrIdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgRVY4OyClZXzsd{kv	NELMNJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
BT-37	MmDHdWhVWyCjc4PhfS=>			NWLoXYR[eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhSlRvM{egZ4VtdHNw	MlT3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
MG 63 (6-TG R)	NFHMTmFyUFSVIHHzd4F6			NX[4bHR{eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVUdiNkOgLFYuXEdiUjmgZ4VtdHNw	MoH4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
Rh30	MXfxTHRUKGG\|c3H5			NXrJdYdveUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhWmh\|MDDj[Yxtey5?	M1vUXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
U-2 OS	NHPNUG5yUFSVIHHzd4F6			MV3xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDVMVIhV1NiY3XscJMv	MojyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
OHS-50	NXHwdIFNeUiWUzDhd5NigQ>?			MVjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDPTHMuPTBiY3XscJMv	Moq1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
Rh41	NG\6V29yUFSVIHHzd4F6			M3vMbJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJpPDFiY3XscJMv	M2e2XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD	NUH0S246eUiWUzDhd5NigQ>?			MXXxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSSEBk\Wyucz6=	MnnRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2	Ml;RdWhVWyCjc4PhfS=>			MnzhdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFOMLVfCUVIh[2WubIOu	M4\NflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC	NGrq[mxyUFSVIHHzd4F6			Mn\kdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIOu	M17USFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1	NFfRS2ZyUFSVIHHzd4F6			MUjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucz6=	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
LAN-5	NUnnSVdFeUiWUzDhd5NigQ>?			NVnMeHZneUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC\|Y4Ll[Y4h\m:{IFzBUk02KGOnbHzzMi=>	NFPqc4g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
Rh18	M4rs[5FJXFNiYYPzZZk>			M1rQdJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCUaEG4JINmdGy\|Lh?=	M{Th[\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643	MYfxTHRUKGG\|c3H5			M2Kx[JFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG5DOTZ2MzDj[Yxtey5?	NHfCRYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N\|UyOzl:L3G+
SK-N-MC	M4LMNJFJXFNiYYPzZZk>			NFTnOHpyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLV3DJINmdGy\|Lh?=	MoTCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN\|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2	NIrneXpyUFSVIHHzd4F6			NYK3OnN2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucz6=	M1\pRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
TC32	MVjxTHRUKGG\|c3H5			MnnQdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[\|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsd{4>	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR\|NUGzPUc,Ojl2M{WxN\|k9N2F-
Rh18	NGXMNmdyUFSVIHHzd4F6			MW\xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW\|IH\vdkBlenWpIILldJVzeG:\|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDSbFE5KGOnbHzzMi=>	M4eyVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2	MV7xTHRUKGG\|c3H5			NUjWNoRoeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy\|Lh?=	M4TxN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
HeLa	NFjtcXhHfW6ldHnvckBie3OjeR?=	MX[xJJVO	MljQOkBpenN?	NHTPO5FKdmirYnn0bY9vKG:oIFjERWMyN0iGQVOyM2hFSUN\|IHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iaX70doFk\WyudXzhdkBi[2W2eXytbIl{fG:wZTDIOEBt\X[nbIOgZZQhOSC3TTDh[pRmeiB4IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm\|aYOu	NF3wUGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUe4O\|I3Oid-Mkm3PFczPjJ:L3G+
MV4-11	MWfBdI9xfG:\|aYOgZZN{[Xl?	M2DTWlUhfU1?	NGHoc28zPCC2bzC0PEBpenN?	NVTxbVBbUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDNWlQuOTFiY3XscJMh[XRiNTD1UUBi\nSncjCyOEB1dyB2ODDodpMh[nliQX7u[ZhqdiCYLWDJJJN1[WmwaX7nJIJie2WmIH\sc5ch[3m2b33leJJ6Ng>?	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd\|OEm1N{c,Ojl5M{i5OVM9N2F-
MV4-11	Ml6ySpVv[3Srb36gZZN{[Xl?	MXSyNFAhdk1?	MV:yOEBpenN?	MU\Jcohq[mm2aX;uJI9nKGOuYYPzJFEhUESDQzDpckBpfW2jbjDNWlQuOTFiY3XscJMh[XO\|ZYPz[YQh[XNiaInw[ZJi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KDJyMDDuUUBi\nSncjCyOEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{Ng>?	MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd\|OEm1N{c,Ojl5M{i5OVM9N2F-
HL60	M3Twc2Fxd3C2b4Ppd{Bie3OjeR?=	NWKzeY5CPSC3TR?=	NVz0dHNKPDhiaILz	MofNTY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCKTE[wJINmdGy\|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKHC{b3Phd5Bie2VvMzDs[ZZmdCCjdDC1JJVOKGGodHXyJFQ5KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXNw	M2S3XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEG1NFA6Lz5{OESxOVAxQTxxYU6=
HL60	MWHBdI9xfG:\|aYOgZZN{[Xl?	MmHNOUB2VQ>?	NFLYdWo1QCCqcoO=	NFPiPYFKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m\|IHnuJIh2dWGwIFjMOlAh[2WubIOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iY3zlZZZm\CClYYPwZZNmNTNibHX2[Ywh[XRiNTD1UUBi\nSncjC0PEBpenNiYomgW4V{fGW{bjDicI91KGGwYXz5d4l{Ng>?	MoLpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2MUWwNFkoRjJ6NEG1NFA6RC:jPh?=
HL60	MnH4RZBweHSxc3nzJIF{e2G7	NV3RO5BCPSC3TR?=	NIntcJA1QCCqcoO=	M4LPbmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iSFy2NEBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiClbHXheoVlKFCDUmCgcIV3\WxiYYSgOUB2VSCjZoTldkA1QCCqcoOgZpkhX2W\|dHXyckBjdG:2IHHuZYx6e2m\|Lh?=	MkXuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2MUWwNFkoRjJ6NEG1NFA6RC:jPh?=
MV4-11	M3;WOmNmdGxiY4njcIUh[XK{ZYP0JIF{e2G7	M2HWfVUhfU1?	MnPBNlQhcHK\|	NGG3XGtE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBOXjRvMUGgZ4VtdHNiYYPz[ZN{\WRiYYOgZYNkfW23bHH0bY9vKGG2IIP1Zk1IOSCyaHHz[UBifCB3IIXNJIFnfGW{IEK0JIhzeyCkeTDwdo9xcWSrdX2gbY9lcWSnIIP0ZYlvcW6pLXLhd4VlKG[ub4egZ5l1d22ndILpZ{Bu\XSqb3Su	NFHaNYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
HEL	NULpOnVpS2WubDDjfYNt\SCjcoLld5Qh[XO\|YYm=	Mmi3OUB2VQ>?	NH7OeYYzPCCqcoO=	Mn:zR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gTGVNKGOnbHzzJIF1KDVidV2gZYZ1\XJiMkSgbJJ{KGK7IIDyc5Bq\Gm3bTDpc4Rq\GVic4ThbY5qdmdvYnHz[YQh\myxdzDjfZRwdWW2cnnjJI1mfGixZD6=	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6OUS0NUc,Ojl3OEm0OFE9N2F-
Jurkat	MmXBR4VtdCCleXPs[UBienKnc4SgZZN{[Xl?	MkLUOUB2VQ>?	NIrofoYzPCCqcoO=	M{fSTmNmdGxiY4njcIUh[XK{ZYP0JIlvKGi3bXHuJGp2emujdDDj[YxteyCjdDC1JJVOKGGodHXyJFI1KGi{czDifUBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nMYJie2WmIH\sc5ch[3m2b33leJJq[yCvZYToc4Qv	NGDNW\|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW4PVQ1OSd-Mkm1PFk1PDF:L3G+
MV4-11	MlfuSpVv[3Srb36gZZN{[Xl?	NU[4VpYxPSC3TR?=	NHPLVIczPCCqcoO=	Mn\OTY5pcWKrdHnvckBw\iClbHHzd{AyKEiGQVOgbY4hcHWvYX6gUXY1NTFzIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnu[JVkfGmxbjDv[kBpcXO2b37lJIh6eGW{YXPleJlt[XSrb36gZZQhPSC3TTDh[pRmeiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JI1mfGixZD6=	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTV6OUS0NUc,Ojl3OEm0OFE9N2F-
LNCAP	NHnybFVHfW6ldHnvckBie3OjeR?=	MnjXNVAhfU1?	MoP3NlQhcHK\|	MUXJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJGxPS0GSIHPlcIx{KGG\|c3Xzd4VlKGG\|IHnubIljcXSrb36gc4YhTEiWLXnu[JVk\WRiYXzwbIEufHWkdXzpckBl\WGlZYT5cIF1cW:wIHL5JI1m[XO3cnnu[{BqdmO{ZXHz[UBqdiCjbIDoZU11fWK3bHnuJIFk\XS7bHH0bY9vKGG2IEGwJJVOKG2nYYP1doVlKGGodHXyJFI1KGi{czDy[YxifGm4ZTD0c{Bkd262cn;sMi=>	MlfDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5MUe1OFQoRjJ5N{G3OVQ1RC:jPh?=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	AcH3 / AcH4 / HDAC1 / HDAC2 / HDAC3; PubMed: 24203492 Pharm Res HCT-15 or fresh primary NK cells were treated with entinostat for 24 h, and AcH3, AcH4, and HDAC1, 2, and 3 were detected by Western blotting. Cleaved PARP; PubMed: 19148581 J Neurooncol Induction of PARP cleavage in Daoy cells upon exposure to MS-275 was demonstrated by Western blot analyses using antibodies that recognize the cleaved 89 kDa PARP protein. Pan-Actin (45 kDa) was used as a loading control. Caspase 8 ; PubMed: 19148581 J Neurooncol Cleavage of pro-Caspase-8 was demonstrated by Western blotting with extracts prepared from Daoy cells treated with 2.5 µM MS-275 for various time periods. Antibodies specific to human Caspase-8 or the loading control, pan-Actin were used to probe protein expression. DR4 / DR5; PubMed: 19148581 J Neurooncol Western blotting to detect DR4 and DR5 expression upon treatment of Daoy cells with 2.5 µM MS-275 for 4, 8 and 24 h. TopoIIα / TopoIIβ; PubMed: 21254166 Hepatology Upper panel, concentration-dependent effects of AR42, MS-275 and vorinostat on the expression of topoIIα versus topoIIβ after 48 h. Lower panel, time-dependent effect of 0.5 μM AR42 and 5 μM MS-275 on topoIIα versus topoIIβ expression in PLC-5 cells.	24203492 19148581 21254166
Immunofluorescence	phalloidin / snail; PubMed: 29855565 Sci Rep (C) Effect of MS-275 treatment on Snail expression and localization. MCs (mesothelial cells) were treated with MS-275 (250 nM), or DMSO for 48 hr and then stimulated with TGFβ1 (2 ng/ml) for 24 h before processing for immunofluorescence with a monoclonal antibody against Snail. Cell nuclei are shown in blue (Hoechst 33342). Confocal images are shown from one representative experiment of three performed. DR4 / DR5; PubMed: 19148581 J Neurooncol Flow Cytometry to measure cell surface expression of FAS, DR4 and DR5 using PE-labeled antibodies. Cells stained with isotype matched anti-human IgG antibodies served as controls. H3K4Me3 / H3K9Me3; PubMed: 22468166 Mol Cell Pharmacol Immunocytochemical analysis of the effects of AR42, SAHA, and MS-275 at the indicated concentrations on H3K4Me3 (upper panels) and H3K9Me3 (lower panels) after 24 h of treatment in LNCaP cells.	29855565 19148581 22468166
Growth inhibition assay	Cell proliferation; PubMed: 24203492 Pharm Res (A) HCT-15 cells were cultured in indicated concentrations of entinostat for up to 72 h, and proliferation determined by MTT assay as indicated. (B) HCT-15, CCH-OS-D, COL and HOS cells were cultured in indicated concentrations of entinostat for 72 h, and viable cell number was determined using the Vi-cell assay and proliferation index calculated. (C) Freshly-isolated human NK cells were cultured in indicated concentrations of entinostat for up to 72 h, and viability was assessed by flow cytometry for 7-AAD uptake. , P < 0.05, ; P ≤ 0.01; **, P ≤ 0.001 Cell viability; PubMed: 28969017 Oncotarget PANC-1, MIA PaCa-2, BxPC3, CFPAC-1, SUIT2 and SUIT2 Clone 1 cells were plated at a density of 3-5×103 per well in 96-well microtiter plates, allowed to adhere overnight and incubated for 72 hours in the presence of variable concentrations of entinostat (0-100μM). Cell viability was determined by XTT assays. The data presented are the mean values from triplicate wells from two independent experiments ±SE.	24203492 28969017
ELISA	GCP-2 / MCP-2 / MIF ; PubMed: 24241152 Molecules MS-275 and SAHA suppressed the secretion of GCP-2, MCP-2 and MIF in E11 cells. (A) GCP suppression. (B) MCP-2 suppression. (C) MIF suppression. E11 cells were incubated with HDAC inhibitors at various concentrations for 1 h before they were stimulated by LPS. 24 h later, the secretion of GCP-2, MCP-2 and MIF were quantified by ELISA (n = 8). Bars show the mean and SD in (A–C).	24241152

体内研究

MS-275按49 mg/kg剂量作用于除了HCT-15的人类移植瘤都显示出强抗癌活性。^[1]MS-275促进恶性实体瘤和恶性血液病的治疗可能性，及生理和畸变基因表达的调节。^[4]MS-275和IL-2联用，作用于肾细胞癌显示出强抗癌活性，因为降低调节性T细胞和增强脾细胞的表达。^[5]

激酶实验：^[6]	- 合并标准HDAC实验: 用HDAC buffer按1:6稀释鼠肝内的酶。重组人类HDACs按1:4稀释在HDAC buffer中。用于标准HDAC实验，60 μl HDAC buffer和10 μl 稀释的酶溶液在30^oC混合。在HDAC buffer中加入30 μl基底液开始HDAC反应，随后在30^oC下温育30分钟。加入100 μl胰蛋白酶溶液终止反应，胰蛋白酶溶液由溶于50 mM Tris-HCl（pH 为8.0）的10 mg/ml 胰蛋白酶, 100 mM NaCl,及 2 μM TSA组成。30^oC下温育20分钟，通过测定460纳米(λ_ex = 390 nm)处的荧光监测AMC的释放。使用释放的AMC校准荧光强度。用于标准时间过程实验，在初始100 μl HDAC 反应中加入20 pmol 基底物。2-50 pmol 基底物的酶法分析产物获得荧光AMC，通过测量这种荧光AMC来测定 K_m值和 V_max值。使用Hanes 图分析实验数据。记录的AMC信号是针对没有酶而有buffer和基底物的空白区。
细胞实验：^[2]	- 合并 Cell lines: A2780, Calu-3, HL-60, K562, St-4, HT-29, KB-3-1, Capan-1, 4-1St和HCT-15细胞 Concentrations: 10 μM 左右 Incubation Time: 3天 Method: 5×10³个肿瘤细胞接种到96孔板上，加入梯度浓度MS-275培养三天。细胞用0.1 mg/mL中性红在CO₂反应器中染色1小时，测定中性红与50 μL乙醇和150 μL 0.1 M Na₂HPO₄溶解后的OD540,测定IC50值。 (Only for Reference)
动物实验：^[1]	- 合并 Animal Models: 侧腹皮下注射A2780, HT-29, HTC-15, KB-3-1, 4-1St, St-4, Capan-1和Calu-3细胞的裸鼠 Dosages: 12.3, 24.5和49 mg/kg Administration: 每天口服处理一次，每周进行5天，持续4周。 (Only for Reference)

参考文献

- 合并

溶解度 (25°C)

体外	DMSO	75 mg/mL (199.25 mM)
	Water	Insoluble
	Ethanol	Insoluble
体内	从左到右依次将纯溶剂加入产品，现配现用(数据来自Selleck实验检测而非文献)： 2% DMSO+30% PEG 300+ddH2O	10mg/mL

* 溶解度检测是由Selleck技术部门检测的，可能会和文献中提供的溶解度有所差异，这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据 Download Entinostat (MS-275) SDF

分子量	376.41
化学式	C₂₁H₂₀N₄O₃
CAS号	209783-80-2
储存条件	3年-20°C 粉状 2年-80°C 溶于溶剂
别名	SNDX-275

动物体内配方计算器 (澄清溶液)

第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量）

给药剂量

mg/kg

动物平均体重

每只动物给药体积

动物数量

只

第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系Selleck为您提供正确的澄清溶液配方）

% DMSO+ % + % Tween 80+ % ddH₂O

计算重置

计算结果:

工作液浓度： mg/ml；

DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，注：如该浓度超过该批次药物DMSO溶解度，请先联系Selleck）；

体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL ddH₂O，混匀澄清。

注意：1. 首先保证母液是澄清的；
2. 一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。

计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系，公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

质量

浓度

体积

分子量
=

×

×

*在配置溶液时，请务必参考Selleck产品标签上、MSDS / COA（可在Selleck的产品页面获得）批次特异的分子量使用本工具。

稀释计算器

用本工具协助配置特定浓度的溶液，使用的计算公式为：

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入输出 )

C1

V1

C2

V2
×

=

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