Resveratrol (SRT501)

目录号:S1396 别名: trans-Resveratrol 中文名称:白藜芦醇

仅限科研使用

Resveratrol (SRT501, trans-Resveratrol)具有广泛靶点,包括环氧酶(如COX, IC50=1.1 μM)、脂肪氧合酶(LOC, IC50=2.7 μM)、sirtuins和其他蛋白质。它是一种天然的植物抗毒素,具有抗癌,抗炎,降血糖和其他有益心血管的作用。Resveratrol可诱导线粒体自噬、细胞自噬和自噬依赖性的凋亡。

Resveratrol (SRT501) Chemical Structure

CAS: 501-36-0

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1068.72 现货
RMB 804.89 现货
RMB 2551.88 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Resveratrol (SRT501)发表文献51篇:

产品安全说明书

Autophagy抑制剂选择性比较

生物活性

产品描述 Resveratrol (SRT501, trans-Resveratrol)具有广泛靶点,包括环氧酶(如COX, IC50=1.1 μM)、脂肪氧合酶(LOC, IC50=2.7 μM)、sirtuins和其他蛋白质。它是一种天然的植物抗毒素,具有抗癌,抗炎,降血糖和其他有益心血管的作用。Resveratrol可诱导线粒体自噬、细胞自噬和自噬依赖性的凋亡。
靶点
SIRT1 [2]
(Cell-free assay)
SIRT2 [2]
(Cell-free assay)
Quinone reductase 2 [9]
(Cell-free assay)
IKK β [1]
(Cell-free assay)
COX1 [9]
(Cell-free assay)
点击更多
88 nM 1 μM 1.1 μM
体外研究

Resveratro 抑制环氧合酶和脂氧化酶,PKCs和p56lck, ERK1,JNK1,p38,IKK β,Src,STAT3,核苷酸还原酶,DNA聚合酶α和 δ,PKD,PKC α,醌还原酶2,以及芳香化酶的活性,IC50值为0.035-60 μM。Resveratrol也是腺苷酸环化酶和AMPK的活化剂,IC50值分别为0.8 μM和50 μM。Resveratrol作为抑制剂或活化剂,能够减少细胞炎症相关的表现,抑制癌症细胞的生长并诱导细胞凋亡,逆转内皮素-1受激的细胞应答,抑制佛波醇酯诱导的COX-2表达,抑制细胞中DNA合成,抵抗甲萘醌诱导的细胞死亡,并且改善细胞线粒体功能和葡萄糖/脂质代谢。[1]Resveratrol也是抗衰老酶的活化剂。Resveratrol降低作用于乙酰化底物和NAD+的SIRT1的Michaelis常数,通过促进SIRT1依赖性的p53脱乙酰作用增加细胞存活。在酵母中,Resveratrol通过刺激Sir2模拟热量限制,增加DNA稳定性并延长寿命。[2] Resveratrol通过其抗氧化活性,能够有效保护离体的大鼠心脏免受心脏缺血/再灌注损伤,改善发展压和主动脉血流的恢复,降低丙二醛的浓度并减少梗死面积。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HL60 cell line MoXmVJJwdGmoZYLheIlwdiCjc4PhfS=> MVvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdCCuaX7lMEBKSzVyPUWg{txO M3j4XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4Nki2OVQ{Lz5zNk[4OlU1OzxxYU6=
human K562 cell line NXizbGlIWHKxbHnm[ZJifGmxbjDhd5NigQ>? NFnvUXFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFu1OlIh[2WubDDsbY5mKGW6cILld5NqdmdiQnPyMWFjdCxiSVO1NF0zQCEQvF2= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6NkW0N{c,OTZ4OE[1OFM9N2F-
HUVEC NYrRWmtiTnWwY4Tpc44h[XO|YYm= NFnGd28yODBizszN M3m5R|Eh\GG7 NHrsbpNKdmS3Y4Tpc44hd2ZibXn0c4Npd26mcnnhcEBjcW:pZX7ld4l{KGmwIFiyU|IucW6mdXPl[EBJXV[HQzDheEAyODBidX3vcE9NKHC{ZYTy[YF1\WRiZn;yJFEh\GG7IH\vcIxwf2WmIHL5JINwdXCxdX7kJJdie2ixdYSgZY5lKEh{T{KteJJm[XSvZX70JI1m[XO3cnXkJIFnfGW{IEGwJIRigXNiYomgUYl1d1S{YXPr[ZIhWmWmIIP0ZYlvcW6pLXLhd4VlKGG|c3H5 NFnkfJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{i1PVI1QSd-MkO4OVkzPDl:L3G+
Hepa1clc7 MWXDfZRwfG:6aXPpeJkh[XO|YYm= MnTSO|IhcHK| NXywRosxS3m2b4TvfIlkcXS7IHHnZYlve3RibX;1d4UhUGWyYUHjcIM4KGOnbHzzJIFnfGW{IEeyJIhzeyxiSVO1NF0yOc7:TT6= MkW5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTByN{W3OFIoRjFyMEe1O|QzRC:jPh?=
MCF7 NVztXGc{TnWwY4Tpc44h[XO|YYm= NXHqO3JLPiCqcoO= M1XuUGlvcGmkaYTpc44hd2ZicHjvdoJwdCCnc4Tldk1qdmS3Y3XkJI9zdmm2aHnu[UBl\WOjcnLvfJlt[XOnIHnuJIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiNjDodpMtKEmFNUC9NVnPxE1w NX7HVolGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCwO|U4PDJpPkGwNFc2PzR{PD;hQi=>
U937 NYD2boNPTnWwY4Tpc44h[XO|YYm= M3r0OWlvcGmkaYTpc44hd2ZiW{G0R31l\Wi7ZILvZZNkd3KkaXOgZYNq\CC3cIThb4UhcW5iaIXtZY4hXTl|NzDj[YxteyCkeTDNbYNp[WWuaYOtUYVvfGWwIIDsc5QtKEurPUO1{txONg>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTJ5N{e2OEc,OTF{N{e3OlQ9N2F-
U937 NWjaW2pPTnWwY4Tpc44h[XO|YYm= MXLJcohq[mm2aX;uJI9nKDJvW{GsNk0{UCiQKW3k[Y95gS2GLXfseYNwe2VidYD0ZYtmKGmwIHj1cYFvKFV7M{egZ4VtdHNiYomgd4NqdnSrbHzheIlwdiC|cHXjeJJwdWW2comgbY4heHKnc3XuZ4Uhd2Zic3;kbZVuNW[{ZXWgZpVn\mW{ MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTJ5N{e2OEc,OTF{N{e3OlQ9N2F-
HL60 MmXvSpVv[3Srb36gZZN{[Xl? MoLCTY5pcWKrdHnvckBw\iB{LWuxMFIuO0hqTjnd[IVwgHlvRD3ncJVkd3OnIIXweIFs\SCrbjDoeY1idiCKTE[wJINmdGy|IHL5JJNkcW62aXzsZZRqd25ic4DlZ5Rzd22ndIL5JIlvKHC{ZYPlcoNmKG:oIIPv[Il2dS2ocnXlJIJ2\m[nch?= M3rnTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMke3O|Y1Lz5zMUK3O|c3PDxxYU6=
U937 NVnpcVJoTnWwY4Tpc44h[XO|YYm= MXPJcohq[mm2aX;uJI9nKFtzNFPd[IVpgWS{b3HzZ49z[mmlIHHjbYQhfXC2YXvlJIlvKGi3bXHuJHU6OzdiY3XscJMh[nlic3PpcpRqdGyjdHnvckB{eGWldILvcYV1enliaX6gdJJme2WwY3Wgc4Yhe2:maYXtMYZz\WViYoXm[oVz MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTJ5N{e2OEc,OTF{N{e3OlQ9N2F-
HL60 MljESpVv[3Srb36gZZN{[Xl? NVO3fGo5UW6qaXLpeIlwdiCxZjDbNVREZWSnaInkdo9ie2OxcnLpZ{Bi[2mmIIXweIFs\SCrbjDoeY1idiCKTE[wJINmdGy|IHL5JJNkcW62aXzsZZRqd25ic4DlZ5Rzd22ndIL5JIlvKHC{ZYPlcoNmKG:oIIPv[Il2dS2ocnXlJIJ2\m[nch?= MlWzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{N{e3OlQoRjFzMke3O|Y1RC:jPh?=
CHO NYPJW3VjTnWwY4Tpc44h[XO|YYm= M1r2eGJtd2OtYXTlJI9nKHKjdDDHcJV1OSCneIDy[ZN{\WRiaX6gR2hQKGOnbHzzJIF{e2W|c3XkJIF{KGmwaHnibZRqd25ib3[gNk1cOSx{LUPILG4qZWSnb4j5MWQu\2y3Y3;z[UB2eHSja3WgZpkhe2OrboTpcIxifGmxbjDzdIVkfHKxbXX0dpk> NH7XOGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUK3O|c3PCd-MUGyO|c4PjR:L3G+
CHO MnnwSpVv[3Srb36gZZN{[Xl? MVTCcI9kc2GmZTDv[kBz[XRiR3z1eFEh\XiycnXzd4VlKGmwIFPIU{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGuxOGNe\GWqeXTyc4F{[2:{YnnjJIFkcWRidYD0ZYtmKGK7IIPjbY51cWyuYYTpc44he3CnY4Tyc41mfHK7 MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTJ5N{e2OEc,OTF{N{e3OlQ9N2F-
CHO Ml\lSpVv[3Srb36gZZN{[Xl? Mn;ZRoxw[2ujZHWgc4YhcHWvYX6gS4x2fDNiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJFIuYzFuMj2zTEhPMV2mZX;4fU1FNWeudXPvd4UhfXC2YXvlJIJ6KHOlaX70bYxt[XSrb36gd5Bm[3S{b33leJJ6 Mly0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{N{e3OlQoRjFzMke3O|Y1RC:jPh?=
CHO MYDGeY5kfGmxbjDhd5NigQ>? M2L5PWJtd2OtYXTlJI9nKGi3bXHuJGdtfXR|IHX4dJJme3OnZDDpckBEUE9iY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBcOTSFXXTlbJllem:jc3PvdoJq[yCjY3nkJJVxfGGtZTDifUB{[2mwdHnscIF1cW:wIIPw[YN1em:vZYTyfS=> MnjxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{N{e3OlQoRjFzMke3O|Y1RC:jPh?=
HL60 MUTGeY5kfGmxbjDhd5NigQ>? MVTJckB3cXS{bzDpcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCycn;sbYZmemG2aX;uJI9nKEiONkCgZ4VtdHNuIFnDOVA:Pc7:TT6= M{XNWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEe3OVk{Lz5zMki3O|U6OzxxYU6=
RAW264.7 MoTaSpVv[3Srb36gZZN{[Xl? NWLXUJdHUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KG6rdILpZ{BwgGmmZTDwdo9lfWO2aX;uJIlvKGyrcH;wc4x6e2GlY3jhdoll\S2jY4TpeoF1\WRibX;1d4UhdWGlcn;wbIFo\SCUQWeyOlQvPyClZXzsd{whUUN3ME2xOU46|ryPLh?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV3Mke1PUc,OTR3NUK3OVk9N2F-
RAW264.7 MYHGeY5kfGmxbjDhd5NigQ>? NX;TSI03UW6qaXLpeI9zgSCnZn\lZ5Qhd25iUFfFMVIheHKxZIXjeIlwdiCrbjDMVHMue3SrbYXsZZRm\CCUQWeyOlQvPyClZXzsd{whUUN3ME20{txONg>? MojMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTVyOEC5PFgoRjF3MEiwPVg5RC:jPh?=
RAW264.7 NXLPNII{TnWwY4Tpc44h[XO|YYm= M{HxOIlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IGDHSU0zKHC{b3T1Z5Rqd25iaX6gUHBUNXO2aX31cIF1\WRiUlHXNlY1NjdiY3XscJMtKEmFNUC9NlAvQM7:TT6= MkL1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV3MEGwOlQoRjF3NUCxNFY1RC:jPh?=
T47D NGmwPJVHfW6ldHnvckBie3OjeR?= M374c2V{fHKxZ3XubYMh[WO2aY\peJkhcW5iaIXtZY4hXDR5RDDj[YxteyCjc4Pld5Nm\CCjczDkdpVoKGyndnXsJINifXOrbnegd5RqdXWuYYTpc44hd2ZiY3XscEBxem:uaX\ldoF1cW:wIHXxeYl3[WynboSgeI8hOTBicF2g[ZN1emGmaX;sJIJ6KGGuYX3hdkBjdHWnIHHzd4F6NCCDY4Tpeol1gT1yLkCz{txONg>? NHH2eGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUe4O|Q{Pid-MUW3PFc1OzZ:L3G+
MCF7 NUfTWm5LTnWwY4Tpc44h[XO|YYm= NIjZOmtGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW|aX7nJJN1cW23bHH0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbjDldZVqfmGuZX70JJRwKDFyIIDNJIV{fHKjZHnvcEBjgSCjbHHtZZIh[my3ZTDhd5NigSxiQXP0bZZqfHl;MT62{txONg>? NUL5SWJyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3PFc1OzZpPkG1O|g4PDN4PD;hQi=>
MDA-MB-231 NU[zRnM4SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NWf0WHdWUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJJJmeXWrcnXkJIZweiCjboTpdJJwdGmoZYLheIl3\SCjY4Tpeol1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOsJGlEPTB;MkCuOe69VS5? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjJ3ME[zOkc,OTZ{NUC2N|Y9N2F-
U937 NYLnWld3S3m2b4TvfIlkcXS7IHHzd4F6 MWLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDVPVM4KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDXV3QyKHSnc4SsJGlEPTB;MUhOwG0v MmTSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ{NUK5NlQoRjF4MkWyPVI1RC:jPh?=
HL60 MVTBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NYjqR5puSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzMEBKSzVyPUZOwG0v M3vyclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUiwNlA1Lz5zNkW4NFIxPDxxYU6=
HEK293T NE\XcIZHfW6ldHnvckBie3OjeR?= M1nBb2lvcGmkaYTpc44hd2ZiVF7GMYFteGijLXnu[JVk\WRiTl[tb4FxeGGEIHHjeIl3[XSrb36gbY4hUEWNMkmzWEBk\WyuczygTWM2OD1{MN88UU4> NUe4WVVpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlUzPzBpPkG3NVI2OjdyPD;hQi=>
BV2 NF;qRm1HfW6ldHnvckBie3OjeR?= NFXLNHpUfXCycnXzd4lwdiCxZjDMVHMue3SrbYXsZZRm\CCFT2iyJI1TVkFiZYjwdoV{e2mxbjDpckBud3W|ZTDCWlIh[2WubIOgZZQhOjBidV2= NWDrXoFERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexNlUzPzBpPkG3NVI2OjdyPD;hQi=>
RAW 264.7 M4PqcWZ2dmO2aX;uJIF{e2G7 NXnXdIlDUW6qaXLpeIlwdiCxZjDMVHMh[W6mIGTOSk1idHCqYT3hZ5RqfmG2ZXSgUm8heHKxZIXjeIlwdiCrbjDteZJqdmViUlHXJFI3PC55IHPlcIx{NCCLQ{WwQVI{NjYQvF2u MnSyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd{NUO4OFQoRjF5MkWzPFQ1RC:jPh?=
Hepa 1c1c7c1 MnLCSpVv[3Srb36gZZN{[Xl? M17NflEzNjVidV2= NHn5dVkzPCCqcoO= MYfJcoR2[3Srb36gc4YheXWrbn;u[UBz\WS3Y4Thd4UhcW5ibX;1d4UhdXW2YX70JGhmeGFiMXOxZ|dkOSClZXzsd{BifCBzMj61JJVOKGGodHXyJFI1KGi{czDy[YxifGm4ZTD0c{Bkd262cn;sMEBC[3Srdnn0fV0yNjF4zszNMi=> Mni0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd|MU[5NVgoRjF5M{G2PVE5RC:jPh?=
Hepa 1c1c7 MXLGeY5kfGmxbjDhd5NigQ>? M3XhN|EzNjVidV2= M2TR[FI1KGi{cx?= M{L5Umlv\HWldHnvckBw\iCzdXnuc45mKHKnZIXjeIF{\SCjY4Tpeol1gSCrbjDtc5V{\SCKZYDhJFFkOWN5IHPlcIx{KGG2IEGyMlUhfU1iYX\0[ZIhOjRiaILzJJJmdGG2aY\lJJRwKGOxboTyc4wtKEGldHn2bZR6RTFwMkVOwG0v NXLxVI9RRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUezNVY6OThpPkG3N|E3QTF6PD;hQi=>
MC3T3-E1 NYf3[WlmTnWwY4Tpc44h[XO|YYm= NUHxXJNOOSC3TR?= MXHS[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCHUj7EUmEh[2:vcHzlfEBqdiCvb4Xz[UBOSzOWMz3FNUBk\WyuczD0doFve2[nY4Tl[EB4cXSqIHTvcYlv[W62LX7l[4F1cX[nIH31eIFvfCCVcnOg[ZhxemW|c3nvckB3\WO2b4KgZZQhOSC3TTDifUBTXC2SQ2K= NXLiNWloRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MDA-MB-231 Mmr5SpVv[3Srb36gZZN{[Xl? NVG1ZZQ2WmW4ZYLzZYwhd2ZiaX7keYN1cW:wIH;mJGZQYE9|YTDhZ5Rqfmm2eTDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IITyZY5{\mWldHXkJJdqfGhiRl;YU|NiNXOyZXPp[olkKHOvYXzsJIlvfGW{ZnXybY5oKFKQQR?= NX;LO25mRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MDA-MB-231 MmPDSpVv[3Srb36gZZN{[Xl? MoThTY5lfWO2aX;uJI9nKE[RWF:zZUBi[3Srdnn0fUBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzz NX\Lb|RzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 MofmSpVv[3Srb36gZZN{[Xl? NHHl[IsyKHWP NF71eoRT\X[ncoPhcEBw\iCrbnPy[YF{\SCrbjDTdoMhc2mwYYPlJJBpd3OyaH;yfYxifGmxbjDpckBud3W|ZTDNR|NVOy2HMTDj[YxteyCjdDCxJJVOKGmwIIDy[ZNmdmOnIH;mJHBROg>? M13V[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 M123RWZ2dmO2aX;uJIF{e2G7 NInPSnAyKHWP MV3S[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCVcnOgb4lv[XOnIIDoc5NxcG:{eXzheIlwdiCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJIRwdWmwYX70MY5m\2G2aY\lJI12fGGwdDDTdoMh\XiycnXzd4lwdiC4ZXP0c5Ih[XRiMTD1US=> NU[1VGtURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 NHztVoVHfW6ldHnvckBie3OjeR?= NF23R4QyKHWP NUjSflhrUW6lcnXhd4UhcW5iU4LjJItqdmG|ZTDwbI9{eGixconsZZRqd25iaX6gcY92e2ViTVOzWFMuTTFiY3XscJMh[XRiMTD1US=> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 MVTGeY5kfGmxbjDhd5NigQ>? NIHNPXVT\X[ncoPhcEBw\iCrbnPy[YF{\SCrbjDEUmEu[mmwZHnu[{Bi[3Srdnn0fUBw\iCHUnHsdIhiKGmwIH71Z4xm[XJiZYj0doFkfHNib3[gcY92e2ViTVOzWFMuTTFiY3XscJMh[nliZXzlZ5Rzd3Cqb4LleIlkKG2xYnnsbZR6KHOqaX\0JIF{e2G7czDpckBxemW|ZX7j[UBw\iC|aH;yeEBqdnSncn\ldolv\yCHUjDhcJBp[S2UTlG= M1;hbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 MXvGeY5kfGmxbjDhd5NigQ>? MXXS[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCGTlGtZolv\GmwZzDhZ5Rqfmm2eTDv[kBGWmGucHjhJIlvKG63Y3zlZZIh\Xi2cnHjeJMhd2ZibX;1d4UhVUN|VEOtSVEh[2WubIOgZpkh\WynY4Tyc5Bpd3KndHnjJI1w[mmuaYT5JJNpcW[2IHHzd4F6eyCrbjDwdoV{\W6lZTDv[kBKS0lvMUiyO|gx MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
T47D MoLTRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? NF3VbGIyKHWP M17MdWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iVES3SEBk\WyuczDheEAyKHWPIHL5JIZtd3diY4n0c41mfHK7 NFfQOmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
primary osteoblast cells Mn;3SpVv[3Srb36gZZN{[Xl? M1TFcFEhfU1? Mmq0VoV3\XK|YXygc4YhcW6lcnXhd4UhcW5iRWKuSG5CKGOxbYDs[ZghcW5icnH0JJBzcW2jcomgc5N1\W:kbHHzeEBk\WyuczD0doFve2[nY4Tl[EB4cXSqIHTvcYlv[W62LX7l[4F1cX[nIH31eIFvfCCVcnOg[ZhxemW|c3nvckB3\WO2b4KgZZQhOSC3TTDifUBTXC2SQ2K= Mn;TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 NGTDSIJHfW6ldHnvckBie3OjeR?= NXjzTWw1OSC3TR?= MXjS[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCyQl3QNkBmgHC{ZYPzbY9vKGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJIF1KDFidV2gZpkhdHWlaX\ldoF{\SCvZYToc4QhcW5icILld4Vv[2Vib3[gVHAz NFqw[4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 NYXsUVV[TnWwY4Tpc44h[XO|YYm= M4O4VFEhfU1? NXXD[ZJPUW6mdXP0bY9vKG:oIFXSJIF{e2:laXH0bY9vKHerdHigRm1ROiCycn;tc5RmeiCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{BifCBzIIXNJIJ6KEOqaWCgZZN{[Xl? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 NUHsV3dXTnWwY4Tpc44h[XO|YYm= MXOxJJVO MYfS[ZZmenOjbDDv[kBqdmS3Y4Tpc44hcW5iboXjcIVieiC2cnHud4xw[2G2aX;uJI9nKEWUYXzwbIEhcW5ibX;1d4UhVUN|VEOtSVEh[2WubIOgeJJidnOoZXP0[YQhf2m2aDDkc41qdmGwdD3u[YdifGm4ZTDteZRidnRiU4LjJIV5eHKnc4Ppc44hfmWldH;yJIF1KDFidV2= NIPxRZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MG63 MmPzSpVv[3Srb36gZZN{[Xl? MoTHNE4yKHSxIEGwJJVO NUn2VXNyOjRiaIK= MUTJcoNz\WG|ZTDpckBxem:uaX\ldoF1cW:wIH;mJIh2dWGwIF3HOlMh[2WubIOgZZQhOC5zIITvJFExKHWPIHHmeIVzKDJ2IHjyJIJ6KG[ub4egZ5l1d22ndIL5 NXXsfpRtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 M2\LdWZ2dmO2aX;uJIF{e2G7 MYfJcoNz\WG|ZTDpckBFVkFvYnnu[Ilv\yCjY4Tpeol1gSCxZjDFVoFteGijIHnuJI52[2ynYYKg[Zh1emGldIOgc4YhdW:3c3WgUWM{XDNvRUGgZ4VtdHNiYomg[Yxm[3S{b4Doc5JmfGmlIH3vZoltcXS7IIPobYZ1KGG|c3H5dy=> MmLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MCF7 MorQRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? Mn;sNUB2VQ>? NIXK[lNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZZQhOSC3TTDifUBndG:5IHP5eI9u\XS{eR?= NXnjSXE4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 NWr2bFR[TnWwY4Tpc44h[XO|YYm= MUixJJVO MWXS[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCyQl3QNkBmgHC{ZYPzbY9vKGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJJRz[W6|ZnXjeIVlKHerdHig[I9ucW6jboStcoVo[XSrdnWgcZV1[W62IGPyZ{BmgHC{ZYPzbY9vKH[nY4TvdkBifCBzIIXNJIJ6KGy3Y3nm[ZJie2VibXX0bI9l MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 MmnzSpVv[3Srb36gZZN{[Xl? NIjqNWcxNjFidH:gNVAhfU1? M3XWVFch\GG7cx?= MnXETY5kemWjc3WgbY4hSUySIHHjeIl3cXS7IHnuJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KGG2IECuNUB1dyBzMDD1UUBi\nSncjC3JIRigXN? NFfsW5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
primary osteoblast cells MnTCSpVv[3Srb36gZZN{[Xl? NGPKXmExNjFidH:gNVAhfU1? NIq2THA4KGSjeYO= MVnJcoNz\WG|ZTDpckBCVFBiYXP0bZZqfHliaX6gdoF1KHC{aX3hdpkhd3O2ZX;icIF{fCClZXzsd{BifCByLkGgeI8hOTBidV2gZYZ1\XJiNzDkZZl{ MkjOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 M2G4bGZ2dmO2aX;uJIF{e2G7 M4L3ZVEhfU1? M1rMNFczKGi{cx?= Mk\zTY5kemWjc3WgbY4hd3O2ZX;jZYxkcW5ibWLORUBt\X[nbIOgbY4hdW:3c3WgUWM{XDNvRUGgZ4VtdHNiYYSgNUB2VSCjZoTldkA4OiCqcoOgZpkhWlRvUFPS NGrkeng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MG63 NFOzZ45HfW6ldHnvckBie3OjeR?= MoDQNUB2VQ>? NG[wO284OiCqcoO= MkDITY5kemWjc3WgbY4hd3O2ZX;jZYxkcW5ibWLORUBt\X[nbIOgbY4hcHWvYX6gUWc3OyClZXzsd{BifCBzIIXNJIFnfGW{IEeyJIhzeyCkeTDSWE1RS1J? MkfsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
primary osteoblast cells NH;UNJpHfW6ldHnvckBie3OjeR?= MWKxJJVO MV:3NkBpenN? NX3LWFJ4UW6lcnXhd4UhcW5ib4P0[Y9k[WylaX6gcXJPSSCuZY\lcJMhcW5icnH0JJBzcW2jcomgc5N1\W:kbHHzeEBk\WyuczDheEAyKHWPIHHmeIVzKDd{IHjyd{BjgSCUVD3QR3I> NVPiNZN3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 MoHrSpVv[3Srb36gZZN{[Xl? M{nnPVEhfU1? NEfWVnM4OiCqcoO= NXe0RYlDUW6lcnXhd4UhcW5ib4P0[Y9k[WylaX6gcIV3\Wy|IHnuJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KGG2IEGgeW0h[W[2ZYKgO|IhcHK|IHL5JGVNUVOD Ml\IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MG63 MnTNSpVv[3Srb36gZZN{[Xl? NWjC[oo5OSC3TR?= NXzzdHBmPzJiaILz NUjjbZYxUW6lcnXhd4UhcW5ib4P0[Y9k[WylaX6gcIV3\Wy|IHnuJIh2dWGwIF3HOlMh[2WubIOgZZQhOSC3TTDh[pRmeiB5MjDodpMh[nliRVzJV2E> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
primary osteoblast cells NHTXU4FHfW6ldHnvckBie3OjeR?= MnnsNUB2VQ>? MVO3NkBpenN? MnLvTY5kemWjc3WgbY4hd3O2ZX;jZYxkcW5ibHX2[Yx{KGmwIILheEBxemmvYYL5JI9{fGWxYnzhd5Qh[2WubIOgZZQhOSC3TTDh[pRmeiB5MjDodpMh[nliRVzJV2E> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 NISzUGpHfW6ldHnvckBie3OjeR?= MVKxJJVO Mk\VO|IhcHK| MXrJcoNz\WG|ZTDpckBwe3Snb4DvcpRqdiCvUl7BJIxmfmWuczDpckBud3W|ZTDNR|NVOy2HMTDj[YxteyCjdDCxJJVOKGGodHXyJFczKGi{czDifUBTXC2SQ2K= NVXkd2xoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MG63 M3rjUGZ2dmO2aX;uJIF{e2G7 NGe2dYUyKHWP NInlUoQ4OiCqcoO= NEf0RXZKdmO{ZXHz[UBqdiCxc4Tlc5BwdnSrbjDtVm5CKGyndnXsd{BqdiCqdX3hckBOTzZ|IHPlcIx{KGG2IEGgeW0h[W[2ZYKgO|IhcHK|IHL5JHJVNVCFUh?= Mo\XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
primary osteoblast cells NGLubGdHfW6ldHnvckBie3OjeR?= M3LtdFEhfU1? NHnkb5k4OiCqcoO= MYnJcoNz\WG|ZTDpckBwe3Snb4DvcpRqdiCvUl7BJIxmfmWuczDpckBz[XRicILpcYFzgSCxc4Tlc4Jt[XO2IHPlcIx{KGG2IEGgeW0h[W[2ZYKgO|IhcHK|IHL5JHJVNVCFUh?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 MoXVSpVv[3Srb36gZZN{[Xl? NHWydoYyKHWP MYi3NkBpenN? NYPlVlJsUW6lcnXhd4UhcW5ib4P0[Y9xd262aX6gcIV3\Wy|IHnuJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KGG2IEGgeW0h[W[2ZYKgO|IhcHK|IHL5JGVNUVOD NYDiSWZ7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MG63 MmfRSpVv[3Srb36gZZN{[Xl? MXOxJJVO NGTMfIU4OiCqcoO= MYnJcoNz\WG|ZTDpckBwe3Snb4DvcpRqdiCuZY\lcJMhcW5iaIXtZY4hVUd4MzDj[YxteyCjdDCxJJVOKGGodHXyJFczKGi{czDifUBGVEmVQR?= MmTtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
primary osteoblast cells MVTGeY5kfGmxbjDhd5NigQ>? MkjWNUB2VQ>? NIjpNGg4OiCqcoO= NIC1NG9KdmO{ZXHz[UBqdiCxc4Tlc5BwdnSrbjDs[ZZmdHNiaX6gdoF1KHC{aX3hdpkhd3O2ZX;icIF{fCClZXzsd{BifCBzIIXNJIFnfGW{IEeyJIhzeyCkeTDFUGlUSQ>? NFm5SlU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 M{nNb2Z2dmO2aX;uJIF{e2G7 NGrjdW8zPCCqcoO= MoXQTY5pcWKrdHnvckBw\iCWTl[tZYxxcGFvaX7keYNm\CCjcH;weI9{cXNiaX6gcY92e2ViTVOzWFMuTTFiY3XscJMh[W[2ZYKgNlQhcHK|IHL5JIZtd3diY4n0c41mfHK7 NY[4dJpQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells NEHZV5JHfW6ldHnvckBie3OjeR?= Mnu4NlQhcHK| MULJcohq[mm2aX;uJI9nKGW2b4Dvd4ll\S2rbnT1Z4VlKGGyb4D0c5NqeyCrbjDyZZQheHKrbXHyfUBwe3Snb3LsZZN1KGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDmcI94KGO7dH;t[ZRzgQ>? NIHsUYE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
primary osteoblast cells MXPGeY5kfGmxbjDhd5NigQ>? MmjMNlQhcHK| MnXwTY5pcWKrdHnvckBw\iCWTl[tZYxxcGFvaX7keYNm\CCjcH;weI9{cXNiaX6gdoF1KHC{aX3hdpkhd3O2ZX;icIF{fCClZXzsd{Bi\nSncjCyOEBpenNiYomg[oxwfyCleYTvcYV1enl? NVfCRWZYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MCF7 NVnpc2U1TnWwY4Tpc44h[XO|YYm= MXmxJJVO NXjIOVd3WmWmdXP0bY9vKGmwIHHuZ4hwemGpZT3pcoRmeGWwZHXueEBkd2yxbomg[o9zdWG2aX;uJI9nKGi3bXHuJG1ETjdiY3XscJMh[XRiMTD1UUBjgSC|b3\0JIFo[XJiY3;sc456KG[xcn3pcoch[XO|YYm= NV3td49YRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
T47D MWTGeY5kfGmxbjDhd5NigQ>? NEm1emEyKHWP MonDVoVlfWO2aX;uJIlvKGGwY3jvdoFo\S2rbnTldIVv\GWwdDDjc4xwdnliZn;ycYF1cW:wIH;mJIh2dWGwIGS0O2Qh[2WubIOgZZQhOSC3TTDifUB{d2[2IHHnZZIh[2:ub375JIZwem2rbnegZZN{[Xl? MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
primary osteoblast cells NVGzSnhVTnWwY4Tpc44h[XO|YYm= NETKWIwyKHWP MmrkTY5kemWjc3WgbY4hSk2SMjDtVm5CKGyndnXsd{BqdiC{YYSgdJJqdWG{eTDvd5Rmd2KuYYP0JINmdGy|IHH0JFEhfU1iYomgVnQuWEOUIH3leIhw\A>? MnrYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
primary osteoblast cells NF7qSVlHfW6ldHnvckBie3OjeR?= Mn\INUB2VQ>? MWTS[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCHUj7EUmEh[2:vcHzlfEBqdiC{YYSgdJJqdWG{eTDvd5Rmd2KuYYP0JINmdGy|IHH0JFEhfU1iYomgVnQuWEOUIHnuJJBz\XOnbnPlJI9nKFCSMh?= NF3JVos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 NXrpbJFRTnWwY4Tpc44h[XO|YYm= NFHOTlkyKHWP M135O|QhcHK| MlPDTY5kemWjc3WgbY4hSk2SMjDlfJBz\XO|aX;uJIlvKG2xdYPlJG1EO1R|LVWxJINmdGy|IHX4dJJme3OrbnegdGJOWDJibYX0ZY51KGG2IEGgeW0h[W[2ZYKgOEBpenNiYomgcJVkcW[ncnHz[UBu\XSqb3SgdoVt[XSrdnWgeI8h[2:wdILvcC=> M371NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 M37HbmZ2dmO2aX;uJIF{e2G7 M1G3OFEhfU1? MVO0JIhzew>? M3zDfmlv[3KnYYPlJIlvKEKPUEKg[ZhxemW|c3nvckBqdiCvb4Xz[UBOSzOWMz3FNUBk\WyuczDlfJBz\XO|aX7nJJBDVVB{ZHXseIEyKG23dHHueEBifCBzIIXNJIFnfGW{IESgbJJ{KGK7IHz1Z4ln\XKjc3WgcYV1cG:mIILlcIF1cX[nIITvJINwdnS{b3y= M1\JUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 MX;GeY5kfGmxbjDhd5NigQ>? M3zKeFEhfU1? NVTvSJNHWmW4ZYLzZYwhd2ZiaX7keYN1cW:wIH;mJGJOWDJiZYjwdoV{e2mxbjDpckBud3W|ZTDNR|NVOy2HMTDj[YxteyCjdDCxJJVOKGK7IGfld5Rmem5iYnzveEBqdiCycnXz[Y5k\SCxZjDJR2kuOTh{N{iw NFXv[nI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 MnPySpVv[3Srb36gZZN{[Xl? NUDPV2RZOSC3TR?= MWDS[ZZmenOjbDDv[kBqdmS3Y4Tpc44hd2ZiQl3QNkBmgHC{ZYPzbY9vKGmwIH3veZNmKE2FM2SzMWUyKGOnbHzzJIF1KDFidV2gZpkhX2W|dHXyckBjdG:2IHnuJJBz\XOnbnPlJI9nKHOqb4L0JIlvfGW{ZnXybY5oKEWUYXzwbIEuWk6D NV7xUI9HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 MUfGeY5kfGmxbjDhd5NigQ>? M3nQUVEhfU1? NH63O45T\X[ncoPhcEBw\iCrbnT1Z5Rqd25iaX6gcpVkdGWjcjD0doFve2yxY3H0bY9vKG:oIFXSZYxxcGFiaX6gcY92e2ViTVOzWFMuTTFiY3XscJMh[XRiMTD1UUBqdiCycnXz[Y5k\SCxZjDQVFI> M3P4e|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 MX;GeY5kfGmxbjDhd5NigQ>? MoLsNUB2VQ>? MmHPVoV3\XK|YXygc4YhcW6mdXP0bY9vKGmwIFLNVFIh\XiycnXzd4lwdiCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJIRwdWmwYX70MY5m\2G2aY\lJI12fGGwdDDTdoMh\XiycnXzd4lwdiC4ZXP0c5Ih[XRiMTD1UUBjgSCZZYP0[ZJvKGKub4S= M1HtT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 NU\oeWtwTnWwY4Tpc44h[XO|YYm= M{Tl[FEhfU1? MlrrVoV3\XK|YXygc4YhcW6mdXP0bY9vKGmwIFLNVFIh\XiycnXzd4lwdiCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{BifCBzIIXNJIJ6KFenc4Tldo4h[myxdDDpckBxemW|ZX7j[UBw\iCSUEK= MnLVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 MX3GeY5kfGmxbjDhd5NigQ>? NHnwU4YyKHWP MnfNVoV3\XK|YXygc4YhcW6lcnXhd4UhcW5iRWKuSG5CKGOxbYDs[ZghcW5ibX;1d4UhVUN|VEOtSVEh[2WubIOgZZQhOSC3TTDifUBTXC2SQ2KgbY4heHKnc3XuZ4Uhd2ZiUGCy M1vkRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
primary osteoblast cells MUDGeY5kfGmxbjDhd5NigQ>? M171bVEhfU1? NEmxXY9T\X[ncoPhcEBw\iCrbnPy[YF{\SCrbjDwRm1ROiCneIDy[ZN{cW:wIHnuJJJifCCycnntZZJ6KG:|dHXvZoxie3RiY3XscJMhfHKjboPm[YN1\WRid3n0bEBld22rbnHueE1v\WejdHn2[UBufXSjboSgV5JkKGW6cILld5Nqd25idnXjeI9zKGG2IEGgeW0h[nlibIXjbYZmemG|ZTDt[ZRpd2R? NWLzVIZSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells NVznfZhQTnWwY4Tpc44h[XO|YYm= MoXqNUB2VQ>? MUXS[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCyQl3QNkBmgHC{ZYPzbY9vKGmwIILheEBxemmvYYL5JI9{fGWxYnzhd5Qh[2WubIOgZZQhOSC3TTDifUBtfWOrZnXyZZNmKG2ndHjv[EBqdiCycnXz[Y5k\SCxZjDQVFI> NFroZ|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 M2\DeGZ2dmO2aX;uJIF{e2G7 MX3S[ZZmenOjbDDv[kBqdmO{ZXHz[UBqdiCGTlGtZolv\GmwZzDhZ5Rqfmm2eTDv[kBGWmGucHjhJIlvKG63Y3zlZZIh\Xi2cnHjeJMhd2ZibX;1d4UhVUN|VEOtSVEh[2WubIOgeJJidnOoZXP0[YQhf2m2aDDkc41qdmGwdD3u[YdifGm4ZTDteZRidnRiU4LjJIV5eHKnc4Ppc44hfmWldH;yJIJ6KGWuZXP0do9xcG:{ZYTpZ{Bud2KrbHn0fUB{cGmodDDhd5NigXN? M4PVfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 M3zXSmZ2dmO2aX;uJIF{e2G7 MlXEVoV3\XK|YXygc4YhcW6lcnXhd4UhcW5iRF7BMYJqdmSrbnegZYN1cX[rdImgc4YhTVKjbIDoZUBqdiCwdXPs[YFzKGW6dILhZ5R{KG:oIH3veZNmKE2FM2SzMWUyKGOnbHzzJIJ6KGWuZXP0do9xcG:{ZYTpZ{Bud2KrbHn0fUB{cGmodDDhd5NigXNiaX6gdJJme2WwY3Wgc4YhWFB{ MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 NXLMenlUTnWwY4Tpc44h[XO|YYm= Ml3XNUB2VQ>? M322PXJmfmW{c3HsJI9nKGmwY4LlZZNmKGmwIFLNVFIh\XiycnXzd4lwdiCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{BmgHC{ZYPzbY5oKHOvYXzsJIlvfGW{ZnXybY5oKFKQQTDmc5IhTk:[QUGgdJJwfGWrbjDheEAyKHWPIHL5JHdme3Sncn6gZoxwfA>? NWTJRm96RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 NXXHbodyTnWwY4Tpc44h[XO|YYm= NEHYOIgyKHWP NH\QN4pT\X[ncoPhcEBw\iCrbnPy[YF{\SCrbjDwRm1ROiCneIDy[ZN{cW:wIHnuJI1wfXOnIF3DN3Q{NUVzIHPlcIx{KGW6cILld5Nqdmdic33hcIwhcW62ZYLm[ZJqdmdiUl7BJIZweiCIT2jBNUBxem:2ZXnuJIF1KDFidV2gZpkhdHWlaX\ldoF{\SCvZYToc4Q> MkLGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 MWnGeY5kfGmxbjDhd5NigQ>? NUfJZmlwOSC3TR?= Ml3nVoV3\XK|YXygc4YhcW6lcnXhd4UhcW5iRWKuSG5CKGOxbYDs[ZghcW5ibX;1d4UhVUN|VEOtSVEh[2WubIOg[ZhxemW|c3nu[{B{dWGubDDpcpRmem[ncnnu[{BTVkFiZn;yJGZQYEFzIIDyc5RmcW5iYYSgNUB2VSCkeTDSWE1RS1J? NXfIb4c6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MDA-MB-231 NEfLXYdHfW6ldHnvckBie3OjeR?= NGHoO41T\X[ncoPhcEBw\iCrbnjpZol1cW:wIHnuJIFv[2ixcnHn[U1qdmSncHXu[IVvfCClb3zvcpkh\m:{bXH0bY9vKG:oIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMhfHKjboPm[YN1\WRid3n0bEBHV1iRM3Gtd5Bm[2moaXOgd41idGxiaX70[ZJn\XKrbnegVm5CKGK7IIPv[pQh[WejcjDjc4xwdnliZn;ycYlv\yCjc4PhfS=> NEX0VJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MDA-MB-231 M3[4OGZ2dmO2aX;uJIF{e2G7 MY\Jcohq[mm2aX;uJI9nKEmJRj3pcoR2[2WmIF\PXG8{[SCyaH;zdIhwenmuYYTpc44h[XRiVHjyN|IhemW|aXT1[UBqdiCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIJ6KFenc4Tldo4h[myxdB?= MkX2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MDA-MB-231 NIHFcpJHfW6ldHnvckBie3OjeR?= MWHJcohq[mm2aX;uJI9nKEmJRj3pcoR2[2WmIFHreEBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDifUBY\XO2ZYLuJIJtd3R? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MDA-MB-231 NYmxcmFNTnWwY4Tpc44h[XO|YYm= NV;oVVFCWmW4ZYLzZYwhd2ZiaX7keYN1cW:wIH;mJGFsfCCrbnHjeIl3[XSrb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczD0doFve2[nY4Tl[EB4cXSqIHPvcpN1cXS3dHn2[Yx6KGGldHn2ZZRm\CCDa4SgcZV1[W62 NFvhOYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MDA-MB-231 Mm\sSpVv[3Srb36gZZN{[Xl? MU\S[ZZmenOjbDDv[kBqdmS3Y4Tpc44hd2ZiRl;YU|NiKG63Y3zlZZIh[WOldX31cIF1cW:wIHnuJIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNidILhcpNn\WO2ZXSge4l1cCClb37zeIl1fXSrdnXsfUBi[3SrdnH0[YQhSWu2IH31eIFvfA>? MljJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MDA-MB-231 Mln1SpVv[3Srb36gZZN{[Xl? NX6wUpRbWmW4ZYLzZYwhd2ZiaX7keYN1cW:wIH;mJGZQYE9|YTDhZ5Rqfmm2eTDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IITyZY5{\mWldHXkJJdqfGhiY3;ud5RqfHW2aY\lcJkh[WO2aY\heIVlKEGtdDDteZRidnR? NE[3T|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MDA-MB-231 NUDwXHdqTnWwY4Tpc44h[XO|YYm= NVvLcnBbTW6qYX7j[Y1mdnRib3[gRYt1KGmwYXP0bZZifGmxbjDpckBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IITyZY5{\mWldHXkJJdqfGhiZH;tbY5idnRibnXnZZRqfmViQXv0JI12fGGwdB?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MDA-MB-231 NEKyPVhHfW6ldHnvckBie3OjeR?= NVf2cIZxTW6qYX7j[Y1mdnRib3[gSm9ZVzOjIH71Z4xm[XJiYXPjeY12dGG2aX;uJIlvKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgeJJidnOoZXP0[YQhf2m2aDDkc41qdmGwdDDu[YdifGm4ZTDBb5QhdXW2YX70 M{PaV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MDA-MB-231 NEXofFRHfW6ldHnvckBie3OjeR?= MWTFcohidmOnbXXueEBw\iCIT2jPN4Eh[WO2aY\peJkhcW5iaIXtZY4hVUSDLV3CMVI{OSClZXzsd{B1emGwc3\lZ5Rm\CC5aYToJIRwdWmwYX70JI5m\2G2aY\lJGFsfCCvdYThcpQ> MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MDA-MB-231 NFnxWXpHfW6ldHnvckBie3OjeR?= MUKxNEBu\y:tZx?= MWGyJIRigXN? MmLiSIVkemWjc3WgbY4hSWu2IHHjeIl3[XSrb36gbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDlfJBz\XO|aX7nJGZQYE9|YT3zdIVkcW[rYzDzcYFtdCCrboTldoZmemmwZzDSUmEhgGWwb3fyZYZ1\WRiaX6gcpVl\SCvaXPlJIF1KDFyIH3nM4toKG[xcjCyJIRigXN? NEn3XYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MDA-MB-231 NEi2dolHfW6ldHnvckBie3OjeR?= NULabo14OTBibXevb4c> MWSyJIRigXN? M3rib2lv\HWldHnvckBw\iCIT2jPN4EhdnWlbHXhdkBi[2O3bYXsZZRqd25iaX6gbJVu[W5iTVTBMW1DNTJ|MTDj[YxteyCIT2jPN4Eue3CnY3nmbYMhe22jbHygbY51\XKoZYLpcochWk6DIIjlco9oemGodHXkJIlvKG63ZHWgcYlk\SCjdDCxNEBu\y:tZzDmc5IhOiCmYYnz NVn1Z2dFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MDA-MB-231 MVXBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? Mnq1NUB2VQ>? MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[XRiMTD1UUBjgSCobH;3JIN6fG:vZYTyfS=> NYjTU4w6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MDA-MB-231 MmrlSpVv[3Srb36gZZN{[Xl? NWHHSZI6OSC3TR?= M2\XW3Jm\HWldHnvckBqdiCjbnPoc5Ji\2VvaX7k[ZBmdmSnboSgZ49td267IH\vdo1ifGmxbjDv[kBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHH0JFEhfU1iYomgd49nfCCjZ3HyJINwdG:weTDmc5JucW6pIHHzd4F6 Ml[wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
MC3T3-E1 M3:zTmZ2dmO2aX;uJIF{e2G7 M2jnfVEhfU1? M4rXbVQ5KGi{cx?= M3S4fWlvcGmkaYTpc44hd2ZiaX7keYN1cW:wIH;mJGFNWCCjY4Tpeol1gSCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{BifCBzIIXNJIFnfGW{IES4JIhzeyCrbjDwdoV{\W6lZTDv[kBDVVB{LYPw[YNq\mmlIH7leZRz[Wyrennu[{BidnSrYn;kfS=> NWHiWG5ERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells M3zLVmZ2dmO2aX;uJIF{e2G7 MX6xJJVO NWm5Xo9uPDhiaILz NEXiT4dKdmirYnn0bY9vKG:oIHnu[JVkfGmxbjDv[kBCVFBiYXP0bZZqfHliaX6gdoF1KHC{aX3hdpkhd3O2ZX;icIF{fCClZXzsd{BifCBzIIXNJIFnfGW{IES4JIhzeyCrbjDwdoV{\W6lZTDv[kBDVVB{LYPw[YNq\mmlIH7leZRz[Wyrennu[{BidnSrYn;kfS=> M4rtWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUGzPFY4Lz5zN{WxN|g3PzxxYU6=
MC3T3-E1 NYrYO5U1TnWwY4Tpc44h[XO|YYm= NHW0OnMyKHWP NFuyN3U1QCCqcoO= NIHvdm9KdmirYnn0bY9vKG:oIHnu[JVkfGmxbjDv[kBCVFBiYXP0bZZqfHliaX6gcY92e2ViTVOzWFMuTTFiY3XscJMh[XRiMTD1UUBi\nSncjC0PEBpenNiaX6gdJJme2WwY3Wgc4Yhdm:pZ3nu NF7JOG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
primary osteoblast cells MXnGeY5kfGmxbjDhd5NigQ>? MlfDNUB2VQ>? M4XPNFQ5KGi{cx?= M{fUT2lvcGmkaYTpc44hd2ZiaX7keYN1cW:wIH;mJGFNWCCjY4Tpeol1gSCrbjDyZZQheHKrbXHyfUBwe3Snb3LsZZN1KGOnbHzzJIF1KDFidV2gZYZ1\XJiNEigbJJ{KGmwIIDy[ZNmdmOnIH;mJI5w\2erbh?= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
primary osteoblast cells MX;BcpRq[XCxcITveIlkKGGldHn2bZR6KGG|c3H5 NYXYSYhIOSC3TR?= M1HzOFQ5KGi{cx?= MWDJcohq[mm2aX;uJI9nKGGwdHnhdI9xfG:2aXOgZYN1cX[rdImgbY4hemG2IIDybY1ienlib4P0[Y9jdGG|dDDj[YxteyCjdDCxJJVOKGGodHXyJFQ5KGi{czDpckBxemW|ZX7j[UBw\iCETWCyMZNx\WOrZnnjJI5mfXS{YXzpfolv\yCjboTpZo9lgQ>? MmXHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
primary osteoblast cells Ml\lRY51cWGyb4D0c5Rq[yCjY4Tpeol1gSCjc4PhfS=> MXqxJJVO Mn\UOFghcHK| NIGzc5pKdmirYnn0bY9vKG:oIHHueIlieG:ydH;0bYMh[WO2aY\peJkhcW5icnH0JJBzcW2jcomgc5N1\W:kbHHzeEBk\WyuczDheEAyKHWPIHHmeIVzKDR6IHjyd{BqdiCycnXz[Y5k\SCxZjDuc4docW5? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 M{fET2Z2dmO2aX;uJIF{e2G7 NFT5RYgxNjFidH:gNVAhfU1? MUCyOEBpeg>? MlK4TY5kemWjc3WgbY4heHKxbHnm[ZJifGmxbjDv[kBud3W|ZTDNR|NVOy2HMTDj[YxteyCjc4Pld5Nm\CCjczDmdoFkfGmxbjDv[kBk\WyuczDpckBUKHCqYYPlJIF1KDBwMTD0c{AyOCC3TTDh[pRmeiB{NDDodkBjgSCobH;3JIN6fG:vZYTyfS=> NXPwRpdZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
primary osteoblast cells NH7CNFBHfW6ldHnvckBie3OjeR?= NH\BWVQxNjFidH:gNVAhfU1? M4n4NVI1KGi{ NWHkOYZTUW6lcnXhd4UhcW5icILvcIln\XKjdHnvckBw\iC{YYSgdJJqdWG{eTDvd5Rmd2KuYYP0JINmdGy|IHH0JFAvOSC2bzCxNEB2VSCjZoTldkAzPCCqcjDifUBndG:5IHP5eI9u\XS{eR?= NXPodGRjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MG63 Mn\rSpVv[3Srb36gZZN{[Xl? MXewMlEhfG9iMUCgeW0> MkTLO{Bl[Xm| MonWTY5kemWjc3WgbY4hSUySIHHjeIl3cXS7IHnuJIh2dWGwIF3HOlMh[2WubIOgZZQhOC5zIITvJFExKHWPIHHmeIVzKDdiZHH5dy=> NUnv[olSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NVM5PjdpPkG3OVE{QDZ5PD;hQi=>
MC3T3-E1 MX7GeY5kfGmxbjDhd5NigQ>? MlvCNlQhcHK| MVHJcohq[mm2aX;uJI9nKGW2b4Dvd4ll\S2rbnT1Z4VlKGGyb4D0c5NqeyCrbjDtc5V{\SCPQ{PUN{1GOSClZXzsd{Bi\nSncjCyOEBpenNiYomg[oxwfyCleYTvcYV1enl? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVzM{i2O{c,OTd3MUO4Olc9N2F-
MC3T3-E1 M3TqWGFvfGmjcH;weI91cWNiYXP0bZZqfHliYYPzZZk> M4P6N|EhfU1? MoH5OFghcHK| MVnJcohq[mm2aX;uJI9nKGGwdHnhdI9xfG:2aXOgZYN1cX[rdImgbY4hdW:3c3WgUWM{XDNvRUGgZ4VtdHNiYYSgNUB2VSCjZoTldkA1QCCqcoOgbY4heHKnc3XuZ4Uhd2ZiQl3QNk1{eGWlaX\pZ{Bv\XW2cnHsbZpqdmdiYX70bYJw\Hl? NFvDN2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{WxN|g3Pyd-MUe1NVM5Pjd:L3G+
MC3T3-E1 MmnXRY51cWGyb4D0c5Rq[yCjY4Tpeol1gSCjc4PhfS=> Ml;LNUB2VQ>? NUTnNYhUPDhiaILz MWLJcohq[mm2aX;uJI9nKGGwdHnhdI9xfG:2aXOgZYN1cX[rdImgbY4hdW:3c3WgUWM{XDNvRUGgZ4VtdHNiYYSgNUB2VSCjZoTldkA1QCCqcoOgbY4heHKnc3XuZ4Uhd2Zibn;n[4lv MmDOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MUO4OlcoRjF5NUGzPFY4RC:jPh?=
RAW264.7 NXnq[oV{TnWwY4Tpc44h[XO|YYm= NFHnXHFKdmirYnn0bY9vKG:oIIDoZYdw[3m2b4Ppd{Bw\iCISWTDMYNwdmq3Z3H0[YQhTXOlaHXybYNpcWFiY3;sbUBMOTJiaX6gcY92e2ViUlHXNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJJJifGmxIH;mJI52dWKncjDv[kBj[WO2ZYLpZYwh[2WubIOgeI8heGijZ3;jfZRmeyC3c3nu[{B1enmyYX6gZox2\SC|dHHpcolv\yCkeTDmcI94KGO7dH;t[ZRzgQ>? Mn\YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7M{ixPFcoRjF5OUO4NVg4RC:jPh?=
RAW264.7 MofjSpVv[3Srb36gZZN{[Xl? MmXCTY5pcWKrdHnvckBw\iCyaHHnc4N6fG:|aYOgc4YhTkmWQz3jc45rfWejdHXkJHN1[XCqeXzvZ49k[3W|IHH1doV2eyB{MEnQJIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC{YYTpc{Bw\iCwdX3i[ZIhd2ZiYnHjeIVzcWGuIHPlcIx{KHSxIIDoZYdw[3m2ZYOgeZNqdmdidIL5dIFvKGKudXWgd5RicW6rbnegZpkh\myxdzDjfZRwdWW2com= M{[5W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUO4NVg4Lz5zN{mzPFE5PzxxYU6=
THP1 NXLOcFdHTnWwY4Tpc44h[XO|YYm= M2W3SmlvcGmkaYTpc44hd2ZicHjh[49kgXSxc3nzJI9nKE[LVFOtZ49vcnWpYYTl[EBGe2OqZYLpZ4hq[SClb3zpJGsyOiCrbjDoeY1idiCWSGCxJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiC{YYTpc{Bw\iCwdX3i[ZIhd2ZiYnHjeIVzcWGuIHPlcIx{KHSxIIDoZYdw[3m2ZYOgeZNqdmdidIL5dIFvKGKudXWgd5RicW6rbnegZpkh\myxdzDjfZRwdWW2com= Mnv5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7M{ixPFcoRjF5OUO4NVg4RC:jPh?=
THP1 NELJNoVHfW6ldHnvckBie3OjeR?= NEHGcHNKdmirYnn0bY9vKG:oIIDoZYdw[3m2b4Ppd{Bw\iCISWTDMYNwdmq3Z3H0[YQhW3SjcHj5cI9kd2OldYOgZZVz\XW|IEKwPXAhcW5iaIXtZY4hXEiSMTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hemG2aX:gc4YhdnWvYnXyJI9nKGKjY4TldolidCClZXzsd{B1dyCyaHHnc4N6fGW|IIXzbY5oKHS{eYDhckBjdHWnIIP0ZYlvcW6pIHL5JIZtd3diY4n0c41mfHK7 MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl|OEG4O{c,OTd7M{ixPFc9N2F-
THP1 MlfmSpVv[3Srb36gZZN{[Xl? M2XkSlEhcHJ? NWnBS2VuUW6qaXLpeIlwdiCxZjDGV2wuOS2rbnT1Z4VlKGmwY4LlZZNmKGmwIIDoZYdw[3m2b4Ppd{Bw\iCISWTDMYNwdmq3Z3H0[YQhTXOlaHXybYNpcWFiY3;sbUBMOTJiaX6gbJVu[W5iVFjQNUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iboXtZoVzKG:oIHPlcIx{KHCqYXfvZ5l1d3OrbnegZoFkfGW{aXGgdIVzKGOnbHygZYZ1\XJiMTDodkBjgSCFTGPN M2nxR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUO4NVg4Lz5zN{mzPFE5PzxxYU6=
THP1 MYPGeY5kfGmxbjDhd5NigQ>? NFfwR3AyKGi{ NHTuV3JKdmirYnn0bY9vKG:oIF\TUE0yNWmwZIXj[YQhcW6lcnXhd4UhcW5icHjh[49kgXSxc3nzJI9nKE[LVFOtZ49vcnWpYYTl[EBGe2OqZYLpZ4hq[SClb3zpJGsyOiCrbjDoeY1idiCWSGCxJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCwdX3i[ZIhd2ZicHjh[49kgXSxc3nu[{Bj[WO2ZYLpZUB1dyC2b4ThcEBvfW2kZYKgc4Yh[2WubIOgZYZ1\XJiMTDodkBjgSCFTGPN NVexfZM1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5N|gyQDdpPkG3PVM5OTh5PD;hQi=>
HEK293 MWPGeY5kfGmxbjDhd5NigQ>? NX\SWVVLOSCqch?= MlnjTY5pcWKrdHnvckBw\iCqZXH0JItqdGynZDDFd4Np\XKrY3jpZUBkd2yrIFuxNk1qdmS3Y3XkJG5HNWujcIDhRkBi[3SrdnH0bY9vKGmwIFjFT|I6OyClZXzsd{BmgHC{ZYPzbY5oKFSOUkKgdJJmfHKnYYTl[EBnd3JiMTDodkBj\W[xcnWgOkBpenNiaHXheEBscWyuZXSgZoFkfGW{aXHsJJN1cW23bHH0bY9vKGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfS=> NIXoRXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mzPFE5Pyd-MUe5N|gyQDd:L3G+
HEK293 M2r1fGZ2dmO2aX;uJIF{e2G7 M{nPPFEhcHJ? M4PFdmlvcGmkaYTpc44hd2ZiaHXheEBscWyuZXSgV5RieGi7bH;jc4NkfXNiYYXy[ZV{KDJyOWCtbY5lfWOnZDDOSk1s[XCyYVKgZYN1cX[jdHnvckBqdiCKRVuyPVMh[2WubIOg[ZhxemW|c3nu[{BVVFJ{IIDy[ZRz\WG2ZXSg[o9zKDFiaIKgZoVnd3KnIE[gbJJ{KGinYYSgb4ltdGWmIHLhZ5RmemmjbDDzeIlufWyjdHnvckBjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYm= NGXkfIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mzPFE5Pyd-MUe5N|gyQDd:L3G+
HEK293 MV7GeY5kfGmxbjDhd5NigQ>? NW\LZmdFOTByIIXN MYqxJIhz NUPQcJVlUW6qaXLpeIlwdiCxZjDo[YF1KGurbHzl[EBGe2OqZYLpZ4hq[SClb3zpJGsyOi2rbnT1Z4VlKG63Y3zlZZIhfHKjboPsc4NifGmxbjDv[kBxPjViZXzlcYVvfCCxZjDOSk1s[XCyYVKgbY4hUEWNMkmzJINmdGy|IHX4dJJme3OrbnegWGxTOiCjdDCxNFAhfU1icILleJJm[XSnZDDmc5IhOSCqcjDi[YZwemViNjDodpMhcGWjdDDrbYxt\WRiYnHjeIVzcWGuIIP0bY12dGG2aX;uJIJ6KGmvbYXuc5N1[WmwaX7nJI1mfGixZB?= MofQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7M{ixPFcoRjF5OUO4NVg4RC:jPh?=
HEK293 NVHoV5ZJTnWwY4Tpc44h[XO|YYm= MVmxNFAhfU1? MY[xJIhz M1;UXmlvcGmkaYTpc44hd2ZiaHXheEBscWyuZXSgV5RieGi7bH;jc4NkfXNiYYXy[ZV{KDJyOWCtbY5lfWOnZDDueYNt\WG{IITyZY5{dG:lYYTpc44hd2ZicE[1JIVt\W2nboSgc4YhVkZva3HwdIFDKGmwIFjFT|I6OyClZXzsd{BmgHC{ZYPzbY5oKFSOUkKgZZQhOTByIIXNJJBz\XS{ZXH0[YQh\m:{IEGgbJIh[mWob4LlJFYhcHK|IHjlZZQhc2mubHXkJIJi[3SncnnhcEB{fGmvdXzheIlwdiCkeTDpcY12dm:|dHHpcolv\yCvZYToc4Q> M17xZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUO4NVg4Lz5zN{mzPFE5PzxxYU6=
RAW264.7 NHrEW4RHfW6ldHnvckBie3OjeR?= NGDpXFYyKHSxIEGwNEB2VQ>? Mn6zOkBpenN? NVrjbGtMUW6qaXLpeIlwdiCxZjDo[YF1KGurbHzl[EBGe2OqZYLpZ4hq[SClb3zpJGsyOi2rbnT1Z4VlKFSQRjDhcJBp[SCycn;keYN1cW:wIHnuJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG2IEGgeI8hOTByIIXNJIFnfGW{IE[gbJJ{KGK7IFXMTXNC NVv2bmU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5N|gyQDdpPkG3PVM5OTh5PD;hQi=>
THP1 MV\GeY5kfGmxbjDhd5NigQ>? NXy1doxwOSC2bzCxNFAhfU1? M4ezeVYhcHK| MVzJcohq[mm2aX;uJI9nKGinYYSgb4ltdGWmIFXzZ4hmemmlaHnhJINwdGliS{GyMYlv\HWlZXSgWG5HKGGucHjhJJBzd2S3Y4Tpc44hcW5iaIXtZY4hXEiSMTDj[YxteyCjdDCxJJRwKDFyMDD1UUBi\nSncjC2JIhzeyCkeTDFUGlUSQ>? NI\tZWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mzPFE5Pyd-MUe5N|gyQDd:L3G+
RAW264.7 NUf6W4J[TnWwY4Tpc44h[XO|YYm= NF64WWcyKHSxIEGwNEB2VQ>? MXi2JIhzew>? M4jtdGlvcGmkaYTpc44hd2ZiaHXheEBscWyuZXSgV5RieGi7bH;jc4NkfXNiYYXy[ZV{KDJyOWCtbY5lfWOnZDDUUmYh[WyyaHGgdJJw\HWldHnvckBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDheEAyKHSxIEGwNEB2VSCjZoTldkA3KGi{czDifUBGVEmVQR?= NGXFd|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{mzPFE5Pyd-MUe5N|gyQDd:L3G+
THP1 NUXIdndLTnWwY4Tpc44h[XO|YYm= MXyxJJRwKDFyMDD1US=> NYfDdWk6PiCqcoO= NEXQUZNKdmirYnn0bY9vKG:oIHjlZZQhc2mubHXkJHN1[XCqeXzvZ49k[3W|IHH1doV2eyB{MEnQMYlv\HWlZXSgWG5HKGGucHjhJJBzd2S3Y4Tpc44hcW5iaIXtZY4hXEiSMTDj[YxteyCjdDCxJJRwKDFyMDD1UUBi\nSncjC2JIhzeyCkeTDFUGlUSQ>? NU\qR4lpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe5N|gyQDdpPkG3PVM5OTh5PD;hQi=>
THP1 M2jK[WZ2dmO2aX;uJIF{e2G7 MWSxNFAhfU1? M2XmZ|EhcHJ? NUfxb2hXUW6qaXLpeIlwdiCxZjDGV2wyNWmwZIXj[YQhfXC{ZXf1cIF1cW:wIH;mJG1UWjFibWLORUBmgHC{ZYPzbY9vKGmwIHnuJIh2dWGwIGTIVFEh[2WubIOgZZQhOTByIIXNJIFnfGW{IEGgbJIh[nliUmStVGNT M4TXWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUO4NVg4Lz5zN{mzPFE5PzxxYU6=
THP1 NFTUS41HfW6ldHnvckBie3OjeR?= M2P2S|ExOCC3TR?= NUf0NlkxOSCqch?= M1fqfGlvcGmkaYTpc44hd2ZiRmPMNU1qdmS3Y3XkJJVxemWpdXzheIlwdiCxZjDDSFM3KG2UTlGg[ZhxemW|c3nvckBqdiCrbjDoeY1idiCWSGCxJINmdGy|IHH0JFExOCC3TTDh[pRmeiBzIHjyJIJ6KFKWLWDDVi=> MlfIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd7M{ixPFcoRjF5OUO4NVg4RC:jPh?=
THP1 Mn2wSpVv[3Srb36gZZN{[Xl? MUCxNFAhfU1? MUKxJIhz NXTzV3Z3UW6qaXLpeIlwdiCxZjDGV2wyNWmwZIXj[YQhfXC{ZXf1cIF1cW:wIH;mJGRENVOLR16gcXJPSSCneIDy[ZN{cW:wIHnuJIlvKGi3bXHuJHRJWDFiY3XscJMh[XRiMUCwJJVOKGGodHXyJFEhcHJiYomgVnQuWEOU MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl|OEG4O{c,OTd7M{ixPFc9N2F-
THP1 NVrHV2J{TnWwY4Tpc44h[XO|YYm= NUfq[I13OTByIIXN NVnIVHNHOSCqch?= NUDu[VNKUW6qaXLpeIlwdiCxZjDGV2wyNWmwZIXj[YQhfXC{ZXf1cIF1cW:wIH;mJIRm[3Srbj2xJI1TVkFiZYjwdoV{e2mxbjDpckBqdiCqdX3hckBVUFBzIHPlcIx{KGG2IEGwNEB2VSCjZoTldkAyKGi{IHL5JHJVNVCFUh?= M33CO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5OUO4NVg4Lz5zN{mzPFE5PzxxYU6=
HEK293 NGHqbYJHfW6ldHnvckBie3OjeR?= MXWxNFAhfU1? MnfGTY5pcWKrdHnvckBw\iCGQz3TTWdPKGW6cILld5Nqd25iaX6gTGVMOjl|IHPlcIx{KGW6cILld5NqdmdiRFOtV2lIViCjdDCxNFAhfU1iYomgW4V{fGW{bjDicI91KGGwYXz5d4l{ MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzl|OEG4O{c,OTd7M{ixPFc9N2F-
U2OS NFn0UFVHfW6ldHnvckBie3OjeR?= MWOxNFAhfU1? NW\qdG55SWO2aY\heIlwdiCxZjDTTXJVOSCrbjDoeY1idiCXMl;TJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKHB3MzDk[YFk\XS7bHH0bY9vKGyndnXsJIF1KDFyMDD1US=> NFPRNnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEC0OlQxQSd-MUiwOFY1ODl:L3G+
293 NITqeFNHfW6ldHnvckBie3OjeR?= Mn7LNlQhcHK| MYjJcohq[mm2aX;uJI9nKFSQRnHsdIhiKGmwZIXj[YQhVkZva3HwdIFDKGGldHn2ZZRqd25iaX6gbJVu[W5iMkmzJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGlEPTB;MU[uNe69VS5? MmHjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2OEewOVMoRjF6NEi3NFU{RC:jPh?=
cortical microglial MlnkSpVv[3Srb36gZZN{[Xl? Mnj0OFghcHK| NF\hZnBKdmirYnn0bY9vKG:oIFzQV{1{fGmvdXzheIVlKE6RIILlcIVie2ViaX6gdoF1KGOxcoTpZ4FtKG2rY4Lv[4xq[WxiY3XscJMh[XO|ZYPz[YQh[XNibnn0dol1\SCjY3P1cZVt[XSrb36gZYZ1\XJiNEigbJJ{NCCLQ{WwQVEyNjYQvF2u NH\sbXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEW4PFM1Oyd-MUi1PFg{PDN:L3G+
HL60 NHvFb3NEgXSxdH;4bYNqfHliYYPzZZk> NVfaT5RIPDhiaILz MmDUR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUO2MlPPxE1w MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTJ3MUSyNEc,OTl{NUG0NlA9N2F-
PBL MUTGeY5kfGmxbjDhd5NigQ>? MYixNk42KHWP NHjPWGtKdmirYnn0bY9vKG:oIHj5[JJw\2WwIIDldo95cWSnLXnu[JVk\WRiRF7BJIRidWGpZTDpckBpfW2jbjDQRmwh[2WubIOgZZQhOTJwNTD1UUBjgSCFb33leEBie3OjeR?= M{DaXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MkexO|M1Lz5zOUK3NVc{PDxxYU6=
HL60 NGXDfItHfW6ldHnvckBie3OjeR?= NFv0TYkyPTBidV2= MYG0PEBpenN? M{\sZWlv\HWldHnvckBw\iCGTlGg[pJi\22nboTheIlwdiCrbjDoeY1idiCKTE[wJINmdGy|IHH0JFE2OCC3TTDh[pRmeiB2ODDodpMh[nliZYTobYRqfW1iYoLvcYll\SCkYYPl[EBo\WxiZXzlZ5Rzd3Cqb4Lld4l{ NHW4UnA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUK3NVc{PCd-MUmyO|E4OzR:L3G+
DLKP M4[4eGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M3OzN|Uh\GG7cx?= MlnqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDNVnAyNWW6cILld5NqdmdiaIXtZY4hTEyNUDDj[YxteyCjZoTldkA2KGSjeYOgZpkh[WOrZDDwbI9{eGijdHHz[UBie3OjeTygTWM2OD1zMN88UU4> MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR6MUS2Nkc,OTl2OEG0OlI9N2F-
DLKP-A NG\uTpRCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M3nablUh\GG7cx?= NHvGcYZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JHAu\3BiZYjwdoV{e2mwZzDoeY1idiCGTFvQMWEh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IHHjbYQheGixc4DoZZRie2ViYYPzZZktKEmFNUC9NVXPxE1w M17WZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEixOFYzLz5zOUS4NVQ3OjxxYU6=
HT144 MUPBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NV:5Z4U1PSCmYYnz M4PENmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSGSxOFQh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IHHjbYQheGixc4DoZZRie2ViYYPzZZktKEmFNUC9OFDPxE1w Mmm3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2OEG0OlIoRjF7NEixOFYzRC:jPh?=
C2C12 M2Dp[mZ2dmO2aX;uJIF{e2G7 MWm1NEB2VQ>? MkjBNVYhcHK| MYPJcoNz\WG|ZTDpckBucXSxY3jvcoRzcWGuIH\1coN1cW:wIHnuJI1wfXOnIFOyR|EzKGOnbHzzJIF{e2W|c3XkJIF{KGWwaHHuZ4Vu\W62IH;mJI1qfG:laH;u[JJq[WxiZHXud4l1gSCjdDC1NEB2VSCjZoTldkAyPiCqcoOgZpkh\3KnZX6gSm0h\HmnIIP0doFqdmmwZz3iZZNm\CCobIXvdoV{[2WwY3WgZZN{[Xl? NGrkdZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOU[2N|Q6QCd-MUm2OlM1QTh:L3G+
3T3L1 M3fu[GFvfGmjZHnwc4dmdmmlIHHzd4F6 NEOzXGY4KGSjeYO= NFjRUXZCdnSrYXTpdI9o\W6rYzDhZ5Rqfmm2eTDh[4FqdnO2IH3veZNmKDOWM1yxJINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiZHnm[oVz\W62aXH0bY9vKGGodHXyJFch\GG7czDifUBwcWxvcnXkJG8he3SjaX7pcoctKEmFNUC9N|EvPM7:TT6= M1W3bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7N{W3PFU{Lz5zOUe1O|g2OzxxYU6=
SW480 M2nuN2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NXrXVIRrPDhiaILz NVLoXHJFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTW|Q5OCClZXzsd{Bie3Onc4Pl[EBieyClZXzsJJZq[WKrbHn0fUB2e2mwZzDwdo9xcWSrdX2gbY9lcWSnIIP0ZYlvcW6pIHHmeIVzKDR6IHjyd{whUUN3ME2yNO69VS5? MkC0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB|OUWwNVkoRjJyM{m1NFE6RC:jPh?=
H460 Mnr4RY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? MU[3NkBpenN? NEDidGlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFi0OlAh[2WubIOgZYZ1\XJiN{KgbJJ{NCCLQ{WwQVEzNjoQvF2u NX\2eJB7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0NFk4OjNpPkKwOFA6PzJ|PD;hQi=>
H460 M3G2SWNmdGxiY4njcIUh[XO|YYm= MlnkNlAhfU1? NHi3SJkzPCCqcoO= NIH2R2dE\WyuIHP5Z4xmKGG{cnXzeEBqdiCqdX3hckBJPDZyIHPlcIx{KGG|c3Xzd4VlKGG|IHHjZ5VufWyjdHnvckBifCCJMj;NJJBp[XOnIHH0JFIxKHWPIHHmeIVzKDJ2IHjyd{BjgSCycn;wbYRqfW1iaX;kbYRmKHO2YXnubY5oNWKjc3XkJIZtd3diY4n0c41mfHK7 MlLXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2MEm3NlMoRjJyNEC5O|I{RC:jPh?=
HEK293 MlPwSpVv[3Srb36gZZN{[Xl? M2ftXVQ5KGi{cx?= MXrJcohq[mm2aX;uJI9nKFSQRj3hcJBp[S2rbnT1Z4VlKE6ILXvhdJBiSiCjY4Tpeol1gSCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGGodHXyJFQ5KGi{czDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:OC5zN{ROwG0v M1LtfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUK3PFkyLz5{MEWyO|g6OTxxYU6=
RAW 264.7 MWjGeY5kfGmxbjDhd5NigQ>? NH;PbXczOCCqcoO= MVnJcohq[mm2aX;uJI9nKGmQT2OtcYVlcWG2ZXSgcol1emmlIH;4bYRmKHC{b3T1Z5Rqd25iaX6gUHBUNXO2aX31cIF1\WRibX;1d4UhWkGZIEK2OE44KGOnbHzzJJBz\XS{ZXH0[YQhOzBibXnud{Bj\W[xcnWgUHBUKGOqYXzs[Y5o\SCvZXHzeZJm\CCjZoTldkAzOCCqcoOgZpkhT3KrZYPzJJJm[WO2aX;uJI1mfGixZDDy[YxifGm4ZTD0c{Bkd262cn;sMEBKSzVyPUG1{txONg>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODV{N{i5NUc,OjB3Mke4PVE9N2F-
Hepa 1c1c7 NWnkeGJzTnWwY4Tpc44h[XO|YYm= NFzD[2pKdmirYnn0bY9vKG:oIIH1bY5wdmVicnXkeYN1[XOnIEGgbY4hdW:3c3WgTIVx[SBzY{HjO{Bk\WyuczDhd5Nme3OnZDDhd{BlenWpIHzleoVtKHKncYXpdoVlKHSxIHTveYJt\SCnbor5cYUh[WO2aY\peJkh[nliTWTUJIF{e2G7LDDDSF0zOc7:TT6= NVn2O3ZKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC1Nlc5QTFpPkKwOVI4QDlzPD;hQi=>
RAW264.7 MmrVSpVv[3Srb36gZZN{[Xl? M3r2NVM1KHWP NUf6S41uOTVibXnudy=> NYPuWWtsUW6qaXLpeIlwdiCxZjDMVHMucW6mdXPl[EBqVk:VIHX4dJJme3Orb36gbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYSgN|QhfU1idILlZZRm\CBzNTDtbY5{KGKnZn;y[UBNWFNiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDF6IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5o M1rGSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNUK3PFkyLz5{MEWyO|g6OTxxYU6=
Caco-2 MoC1R5l1d3SxeHnjbZR6KGG|c3H5 Mni1N{Bl[Xm| MV;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDZYNwNTJiY3XscJMh[W[2ZYKgN{Bl[Xm|IHL5JHs{UF22aIntbYRqdmViaX7jc5Jxd3KjdHnvckBie3OjeTygTWM2OD1{ND6zOe69VS5? NEm3[GI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{ME[yO|M4QSd-MkC2Nlc{Pzl:L3G+
HT-29 MWPDfZRwfG:6aXPpeJkh[XO|YYm= NWOyO2FIOyCmYYnz MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{Bi\nSncjCzJIRigXNiYomgX|NJZXSqeX3p[Ilv\SCrbnPvdpBwemG2aX;uJIF{e2G7LDDJR|UxRTR3LkROwG0v MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODZ{N{O3PUc,OjB4MkezO|k9N2F-
MDA-MB-231 M3jSS2FvfGm2dX3vdkBie3OjeR?= MlPaOkBl[Xm| M{nIemFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi1{M{GgZ4VtdHNiYX\0[ZIhPiCmYYnzJIJ6KFOUQjDhd5NigSxiSVO1NF0zOC53zszNMi=> M{K2WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyN{K4N|Y6Lz5{MEeyPFM3QTxxYU6=
CCD-18Co M3XMcWFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? NXXoRXpOOSC3TR?= MnrTNVghcHK| NFHKfmNCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKGi3bXHuJGNETC1zOFPvJINmdGy|IHHzd4V{e2WmIHnubIljcXSrb36gc4YhUUxzYnX0ZU1qdmS3Y3XkJHBITTJicILv[JVkfGmxbjDheEAyKHWPIHHmeIVzKDF6IHjydy=> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh4NkCzNkc,OjB6Nk[wN|I9N2F-
CCD-18Co NFuwNJdCdnSraX7mcIFudWG2b4L5JIF{e2G7 M4jmV|IvPSC2bzCyOUB2VQ>? MUWxPEBpenN? NXLiTIdVSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCqdX3hckBES0RvMUjDc{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFnMNYJmfGFvaX7keYNm\CCSR1WyJJBzd2S3Y4Tpc44h[XRiMj61JJRwKDJ3IIXNJIFnfGW{IEG4JIhzew>? NWHC[5g{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4OlYxOzJpPkKwPFY3ODN{PD;hQi=>
Caco2 NWfq[phOTnWwY4Tpc44h[XO|YYm= NFS2[og3KGi{cx?= Ml;0UYV1[WKxbHnjJJN1[WKrbHn0fUBqdiCqdX3hckBE[WOxMjDj[YxteyCjZoTldkA3KGi{czDifUBNSy2PUz3NV{BidmGueYPpdy=> M1ywPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOE[2NFMzLz5{MEi2OlA{OjxxYU6=
HT22 NWnseHhWVmW3cn;wdo91\WO2aY\lJIF{e2G7 NHjKNoEzPCCqcoO= M3rkd25mfXKxcILveIVkfGm4ZTDhZ5Rqfmm2eTDpckBud3W|ZTDIWFIzKGOnbHzzJIF{e2W|c3XkJIF{KHC{b4TlZ5Rqd25iYXfhbY5{fCCpbIX0ZY1ifGVvaX7keYNm\CCxeIn0c5NqeyCjZoTldkAzPCCqcoOgZpkhVVSWIHHzd4F6NCCHQ{WwQVQvPjZ5zszNMi=> MnLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzMkm5O|goRjJzMUK5PVc5RC:jPh?=
SW480 MmrqR5l1d3SxeHnjbZR6KGG|c3H5 NUTwZ2dyOTBidV2= MlHjNkBl[Xm| M{W4RWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNYPDhyIHPlcIx{KGG2IEGwJJVOKG[xcjCyJIRigXNiYomgeoku[2WubDDj[YxtKH[rYXLpcIl1gSCjbnHsfZNqew>? NGDGbJQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NVI{PSd-MkGyPVEzOzV:L3G+
HCT116 MYLDfZRwfG:6aXPpeJkh[XO|YYm= MYGxNEB2VQ>? NF\Cc4QzKGSjeYO= Mn3yR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGG2IEGwJJVOKG[xcjCyJIRigXNiYomgeoku[2WubDDj[YxtKH[rYXLpcIl1gSCjbnHsfZNqew>? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MUKzOUc,OjF{OUGyN|U9N2F-
HCT116 M2HOT2N6fG:2b4jpZ4l1gSCjc4PhfS=> MW[xNEB2VQ>? MYK0JIRigXN? MlLHR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGG2IEGwJJVOKG[xcjC0JIRigXNiYomgeoku[2WubDDj[YxtKH[rYXLpcIl1gSCjbnHsfZNqew>? M2nscFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS 174T NIXXNnJEgXSxdH;4bYNqfHliYYPzZZk> MXGxNEB2VQ>? NXyx[YlrPCCmYYnz M{fubmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxUKDF5NGSgZ4VtdHNiYYSgNVAhfU1iZn;yJFQh\GG7czDifUB3cS2lZXzsJINmdGxidnnhZoltcXS7IHHuZYx6e2m| NXfufIViRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVEzOzVpPkKxNlkyOjN3PD;hQi=>
LS 174T MUHDfZRwfG:6aXPpeJkh[XO|YYm= M2e0OVExKHWP NI\0O20zKGSjeYO= MlPKR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUHMhOTd2VDDj[YxteyCjdDCxNEB2VSCob4KgNkBl[Xm|IHL5JJZqNWOnbHygZ4VtdCC4aXHibYxqfHliYX7hcJl{cXN? NIHBTHM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NVI{PSd-MkGyPVEzOzV:L3G+
LS174T MnfQSpVv[3Srb36gZZN{[Xl? MX2xNFAhfU1? MlPrN|YhcHK| MoixTY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYPlbY4hcW5iaIXtZY4hVFNzN{TUJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCNaU[3JI1TVkFibHX2[Ywh[XRiMUCwJJVOKGGodHXyJFM3KGi{czDifUBTXC2SQ2KgZY5idHm|aYO= M{WzcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS174T MkT5SpVv[3Srb36gZZN{[Xl? NYGybGxMOTByIIXN MVezOkBpenN? NELnZYxKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPhd4VqdiCrbjDoeY1idiCOU{G3OHQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHOtUZlkKG2UTlGgcIV3\WxiYYSgNVAxKHWPIHHmeIVzKDN4IHjyd{BjgSCUVD3QR3Ih[W6jbInzbZM> M3\xN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS174T NX[5[3llTnWwY4Tpc44h[XO|YYm= NInndoQyODBidV2= NY\1OnByOzZiaILz Mk\wTY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYPlbY4hcW5iaIXtZY4hVFNzN{TUJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCFRES0JI1TVkFibHX2[Ywh[XRiMUCwJJVOKGGodHXyJFM3KGi{czDifUBTXC2SQ2KgZY5idHm|aYO= M2XDUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS174T MmLLSpVv[3Srb36gZZN{[Xl? M324SVExOCC3TR?= MUmzOkBpenN? NHjRWZNKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPhd4VqdiCrbjDoeY1idiCOU{G3OHQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIFzndlUhdVKQQTDs[ZZmdCCjdDCxNFAhfU1iYX\0[ZIhOzZiaILzJIJ6KFKWLWDDVkBidmGueYPpdy=> NXzQTVc4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVEzOzVpPkKxNlkyOjN3PD;hQi=>
LS174T MU\GeY5kfGmxbjDhd5NigQ>? Mm\DNVAxKHWP MV6zOkBpenN? M3vRUGlvcGmkaYTpc44hd2ZiV370M4JmfGFvY3Hz[YlvKGmwIHj1cYFvKEyVMUe0WEBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5ic4Xyeol3cW5ibWLORUBt\X[nbDDheEAyODBidV2gZYZ1\XJiM{[gbJJ{KGK7IGLUMXBEWiCjbnHsfZNqew>? M2HRPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS174T MWPGeY5kfGmxbjDhd5NigQ>? M2H3V|ExOCC3TR?= NIC2OW1KdmirYnn0bY9vKG:oIGfueE9j\XSjLXPhd4VqdiCrbjDoeY1idiCOU{G3OHQh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5icEKxW2FHOS:FSWCxJJBzd3SnaX6gcIV3\WxiYYSgNVAxKHWPIHL5JHdme3Sncn6gZoxwfHSrbne= M4XUblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS174T NVHz[ll3TnWwY4Tpc44h[XO|YYm= NGTnRnIyODBidV2= NFjCSmVKdmirYnn0bY9vKG:oIGfueE9j\XSjLXPhd4VqdiCrbjDoeY1idiCOU{G3OHQh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHP5Z4xqdiCEMTDwdo91\WmwIHzleoVtKGG2IEGwNEB2VSCkeTDX[ZN1\XKwIHLsc5R1cW6p NFfkTmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NVI{PSd-MkGyPVEzOzV:L3G+
LS174T M2T2cWZ2dmO2aX;uJIF{e2G7 MWGxNFAhfU1? NVLQWlNwUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZNmcW5iaX6gbJVu[W5iTGOxO|RVKGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDjMW16[yCycn;0[YlvKGyndnXsJIF1KDFyMDD1UUBjgSCZZYP0[ZJvKGKub4T0bY5o M{jX[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS174T M1v4NmZ2dmO2aX;uJIF{e2G7 NHXINHAyODBidV2= MkXuTY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYPlbY4hcW5iaIXtZY4hVFNzN{TUJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCFeXPsbY4hTDFicILveIVqdiCuZY\lcEBifCBzMECgeW0h[nliV3XzeIVzdiCkbH;0eIlv\w>? MmLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUGyN|UoRjJzMkmxNlM2RC:jPh?=
HEK293T MlzoSpVv[3Srb36gZZN{[Xl? MXexNFAhfU1? MnL2NVIhcHK| MlPtTY5pcWKrdHnvckBw\iCOaVPsMYlv\HWlZXSgW451N2KndHGtZ4F{\WmwIHnuJIh2dWGwIFjFT|I6O1RiY3XscJMhfHKjboPm[YN1\WRid3n0bEBVV1BiRnzhd4gh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gcJVkcW[ncnHz[UBmgHC{ZYPzbY9vKGG2IEGwNEB2VSCjZoTldkAyOiCqcoOgZpkh\HWjbDDseYNq\mW{YYPlJJJmeG:{dHXyJIF{e2G7 M3fxV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
SW480 NWXabmJmS3m2b4TvfIlkcXS7IHHzd4F6 Mn\mNVAhfU1? NEjFfWw1KGSjeYO= MXPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTW|Q5OCClZXzsd{BifCBzMDD1UUBnd3JiNDDkZZl{KGK7II\pMYNmdGxiY3XscEB3cWGkaXzpeJkh[W6jbInzbZM> M1zJVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS 174T NV7t[I4yTnWwY4Tpc44h[XO|YYm= MnvhNVAxKHWP NWHjSlNFUW6qaXLpeIlwdiCxZjDXcpQw[mW2YT3jZZNmcW5iaX6gbJVu[W5iTGOgNVc1XCClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gWGNHPCCycn;0[YlvKGyndnXsJIF1KDFyMDD1UUBjgSCZZYP0[ZJvKGKub4T0bY5o M4PZfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
LS 174T NV76cllQTnWwY4Tpc44h[XO|YYm= MoG2NVAxKHWP MWnJcohq[mm2aX;uJI9nKFewdD;i[ZRiNWOjc3XpckBqdiCqdX3hckBNWyBzN{TUJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCyeXfvdJV{OiCycn;0[YlvKGyndnXsJIF1KDFyMDD1UUBjgSCZZYP0[ZJvKGKub4T0bY5o M1nycVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmxNlM2Lz5{MUK5NVI{PTxxYU6=
HEK293T NYjRSJJOTnWwY4Tpc44h[XO|YYm= M2PzUVExOCC3TR?= NGKyZ|AyOiCqcoO= MlnJTY5pcWKrdHnvckBw\iCZboSvZoV1[S2lYYPlbY4hcW5iaIXtZY4hUEWNMkmzWEBk\WyuczD0doFve2[nY4Tl[EB4cXSqIGTPVEBHdGG|aDDhd5Nme3OnZDDhd{Bl\WO{ZXHz[UBqdiCudXPp[oVz[XOnIHX4dJJme3Orb36gZZQhOTByIIXNJIFnfGW{IEGyJIhzeyCkeTDkeYFtKGy3Y3nm[ZJie2VicnXwc5J1\XJiYYPzZZk> MlrnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUGyN|UoRjJzMkmxNlM2RC:jPh?=
32Dcl3 MkHLSpVv[3Srb36gZZN{[Xl? NV\M[phwOTBidV2= NXnOToRVOSCqch?= NEfwPGhT[WSrb4Dyc5Rm[3SrdnWgZYN1cX[rdImgZYdicW6|dDCwJJRwKDdiR4mg[4FudWFicnHkbYF1cW:wLXnu[JVk\WRiZHXheIghcW5ibX;1d4UhOzKGY3yzJINmdGy|IHH0JFExKHWPIITy[YF1\WRiMTDodkBxemmxcjD0c{BqenKjZHnheIlwdiCjZoTldkA4KGSjeYOgZpkhdWmlcn;zZ49xgQ>? NIjhRZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUiyOlI2Oyd-MkG4NlYzPTN:L3G+
HeLa MnrIRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M2fjZVQ5KGi{cx?= NEjhZZNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IGPSRkBie3OjeTygTWM2OD1yLkCwNFAzOjYQvF2u NHz1XIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi1NVA5Oyd-MkG4OVExQDN:L3G+
SK-N-SH NX;VfohzSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MUW0PEBpenN? MnPmRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVSz3OMXNJKGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;MD6wNFAxPDB|zszNMi=> M1fHNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOEWxNFg{Lz5{MUi1NVA5OzxxYU6=
A549 MVvBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? NYCzOop3PDhiaILz M2W3cGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{Bk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliU2LCJIF{e2G7LDDJR|UxRTBwMECwNFQ1P87:TT6= NVLSV3h5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4OVExQDNpPkKxPFUyODh|PD;hQi=>
MCF7 M3vVSWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NIPh[YM1QCCqcoO= NULlUJdJSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNR2Y4KGOnbHzzJIF{e2W|c3XkJIF{KGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;MD6wNFAxPzlzzszNMi=> NIjYXGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUi1NVA5Oyd-MkG4OVExQDN:L3G+
RAW264.7 M4roZ2FvfGmrbn\sZY1u[XSxcomgZZN{[Xl? MUO1NEB2VQ>? MVKxMlUhcHK| M2HxfmFvfGmrbn\sZY1u[XSxcomgZYN1cX[rdImgbY4hdW:3c3WgVmFYOjZ2LkegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDUUmYu[WyyaHGgdJJw\HWldHnvckBifCB3MDD1UUBi\nSncjCxMlUhcHK|IHL5JGVNUVOD MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjB6OEOwPUc,OjJyOEizNFk9N2F-
HEK293 MkSySpVv[3Srb36gZZN{[Xl? NIO5dIVKdmirYnn0bY9vKG:oIGTOSoFteGijLXHjeIl3[XSnZDDOSk1s[XCyYVKg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDifUBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFnDOVA:OC57ON88UU4> NVS4b|J6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKxNVU5OzlpPkKyNVE2QDN7PD;hQi=>
Hepa-1c1c7 NHv0XYpHfW6ldHnvckBie3OjeR?= NEXtPYJKdmS3Y4Tpc44hd2ZiUWKxJIFkfGm4aYT5JIlvKG2xdYPlJGhmeGFvMXOxZ|ch[2WubIOgeZNqdmdiTWTUJIF{KHO3YoP0doF1\SCkeTDzdIVkfHKxcHjveI9u\XS{eTygR2Q:OjIQvF2u MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzNUizPUc,OjJzMUW4N|k9N2F-
RAW264.7 NX32T|lJSW62aXnu[oxidW2jdH;yfUBie3OjeR?= NWPzeXViOzBibXnudy=> NUHMOGJ2SW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJG5QKHC{b3T1Z5Rqd25icILlbY5kfWKjdHXkJIZweiB|MDDtbY5{KGKnZn;y[UBNWFNiY3jhcIxmdmenIH3lZZN2emWmIHHmeIVzKDJ2IHjyd{BjgSCJcnnld5MhdWW2aH;kMEBKSzVyPUOwMlcy|ryPLh?= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjFzNUizPUc,OjJzMUW4N|k9N2F-
B16F10 NWjmOphNSW62aX3lcIFvd2enbnnjJIF{e2G7 M{jRUFIxOCC3TR?= NIL1TY84OiCqcoO= M2O5eWFvfGmvZXzhco9o\W6rYzDhZ5Rqfmm2eTDpckBud3W|ZTDhd5Nme3OnZDDhd{Bk\WyudXzhdkBu\WyjbnnuJINwdnSnboSgRlE3TjFyIHPlcIx{KGG2IEKwNEB2VSCjZoTldkA4OiCqcoOgd5Bm[3S{b4Doc5RwdWW2cnnjJIFv[Wy7c3nz MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjF6OUK3Nkc,OjJzOEmyO|I9N2F-
HEK293 MUTGeY5kfGmxbjDhd5NigQ>? MnLmTY5pcWKrdHnvckBw\iCWTl[tZYxxcGFiYXP0bZZifGWmIF7GMYtieHCjQjDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHL5JIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiSVO1NF0xNjl6zszNMi=> NVz6O2VbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzPFY2PjRpPkKyN|g3PTZ2PD;hQi=>
Hepa-1c1c7 Mni1SpVv[3Srb36gZZN{[Xl? NHjHTVNKdmS3Y4Tpc44hd2ZiUWKxJIlvKG2xdYPlJGhmeGFvMXOxZ|ch[2WubIOgZZN{\XO|ZXSgZZMh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{Bld3WkbHWgVXIyKGmwZIXjeIlwdiCkeTDNWHQh[XO|YYmsJGNFRTJzzszNMi=> NVHXZ|cxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzPFY2PjRpPkKyN|g3PTZ2PD;hQi=>
RAW264.7 NGDv[WhHfW6ldHnvckBie3OjeR?= NHnKXG4yQCCqcoO= Mn;WTY5pcWKrdHnvckBw\iCrTl;TJIlvKEySUz3zeIlufWyjdHXkJI1wfXOnIGLBW|I3PC55IHPlcIx{KGGodHXyJFE5KGi{czygTWM2OD1{Mz6y{txONg>? NUXMWY9NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzPFY2PjRpPkKyN|g3PTZ2PD;hQi=>
RAW264.7 M1HYcmFvfGmrbn\sZY1u[XSxcomgZZN{[Xl? MnvpN|AhdWmwcx?= MnLnRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44heHKnaX7jeYJifGWmIH\vdkA{OCCvaX7zJI1m[XO3cnXkJFI1KGi{czDwc5N1KEySUzDjbIFtdGWwZ3WgZpkhT3KrZYPzJI1mfGixZDygTWM2OD1|MD63Ne69VS5? NVLNSnY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzPFY2PjRpPkKyN|g3PTZ2PD;hQi=>
NIH/3T3 NFnvS5hEgXSxdH;4bYNqfHliYYPzZZk> MWe5OkBpenN? NITkTVlEgXSxdH;4bYNqfHliYXfhbY5{fCCvb4Xz[UBPUUhxM2SzJINmdGy|IHHmeIVzKDl4IHjyd{BjgSCVUlKgZZN{[XluIFnDOVA:OjRwMkNOwG0v MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjd2OUO5Nkc,OjJ5NEmzPVI9N2F-
HEK293 NHHXXlBHfW6ldHnvckBie3OjeR?= NFTaN4ZKdmirYnn0bY9vKG:oIGTOSoFteGijLXnu[JVk\WRiTl[tb4FxeGGEIHHjeIl3cXS7IHX4dJJme3OnZDDpckBJTUt{OUOgZ4VtdHNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwQVAvQTkQvF2u NGLLXIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G0NlMzOCd-MkOxOFI{OjB:L3G+
Hepa-1c1c7 M1mxc2Z2dmO2aX;uJIF{e2G7 NEnkNXM1QCCqcoO= NVe0[Yk6UW6mdXP0bY9vKG:oIIH1bY5wdmVicnXkeYN1[XOnLUGgZYN1cX[rdImgbY4hdW:3c3WgTIVx[S1zY{HjO{Bk\WyuczDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDDSF0zOc7:TT6= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF2MkOyNEc,OjNzNEKzNlA9N2F-
RAW 264.7 MlnNSpVv[3Srb36gZZN{[Xl? NE\TXGc{OCCvaX7z M4DGT2lvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgbW5QWyCjY4Tpeol1gSCrbjDtc5V{\SCUQWegNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kBPVyCycn;keYN1cW:wIIDy[ZRz\WG2ZXSge4l1cCClb33wc5Vv\CCob4KgN|AhdWmwczDi[YZwemViTGDTJINp[WyuZX7n[UBi\nSncjCyOEBpenNiYomgS5Jq\XO|IILlZYdmdnRibXX0bI9lNCCLQ{WwQVI{NjMQvF2u NHzIdm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G0NlMzOCd-MkOxOFI{OjB:L3G+
MCF7 M{LR[2Z2dmO2aX;uJIF{e2G7 MnjiQlExOCC3TR?= MVnBZ5RqfmG2aX;uJI9nKFOLUmSxJIlvKGi3bXHuJG1ETjdiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIRwgG:{dXLpZ4lvNWmwZIXj[YQh[WOndInsMZA2OyCuZY\lcEBifCB-MUCwJJVOKGGodHXyJFYhcHK|IHL5JHdme3Sncn6gZoxwfHSrbnegZY5idHm|aYO= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzNzNkiwN{c,OjN|MU[4NFM9N2F-
HL60 Mk\pR5l1d3SxeHnjbZR6KGG|c3H5 M3nTbVQ5KGi{cx?= Mo\FR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGw3OCClZXzsd{Bie3Onc4Pl[EBieyCpcn;3eIghcW6qaXLpeIlwdiCvZXHzeZJm\CCjZoTldkA1QCCqcoOgZpkhYFSWIHHzd4F6NCCLQ{WwQVMyNjkQvF2u M{XkZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUS3PFQ{Lz5{M{W0O|g1OzxxYU6=
HeLa M{PWU2Z2dmO2aX;uJIF{e2G7 NHjWeJRKdmirYnn0bY9vKG:oIIPtZYQ{NzRic3nncoFtcW6pIIDheIh4[XliaX6gbJVu[W5iSHXMZUBk\WyuczDhd5Nme3OnZDDhd{Bu\WG|dYLl[EBieyCub4fld5Qh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDC1NEB1dyB4MDClJIlvcGmkaYTpc44hd2ZiVFfGMYJmfGFiaX7keYNm\CCudXPp[oVz[XOnIITy[YF1\WRiM{CgcYlveyCkZX\vdoUhcW6mdXP0bY9vNCCPRVS9N|PPxE1w NUjWZZZ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1OFc5PDNpPkKzOVQ4QDR|PD;hQi=>
HeLa M2npN2Z2dmO2aX;uJIF{e2G7 MlnYTY5pcWKrdHnvckBw\iCKZXTn[Yhw\yC|aXfuZYxqdmdicHH0bJdigSCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJI1m[XO3cnXkJIF{KGyxd3XzeEBkd26lZX70doF1cW:wIILldZVqemWmIEWwJJRwKDZyIDWgbY5pcWKrdHnvckBw\iCSTVGgbY5lfWOnZDDseYNq\mW{YYPlJJRz\WG2ZXSgN|AhdWmwczDi[YZwemViaX7keYN1cW:wLDDNSWQ:PDEQvF2u NHfET5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W0O|g1Oyd-MkO1OFc5PDN:L3G+
HeLa Mn6xSpVv[3Srb36gZZN{[Xl? M1;kcWlvcGmkaYTpc44hd2ZiSHXk[4Vpd2dic3nncoFtcW6pIIDheIh4[XliaX6gbJVu[W5iSHXMZUBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIGDNRUBqdmS3Y3XkJIx2[2moZYLhd4UhfHKnYYTl[EA{OCCvaX7zJIJm\m:{ZTDpcoR2[3Srb36sJGlEPTB;NEFOwG0v MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV2N{i0N{c,OjN3NEe4OFM9N2F-
HeLa MkHHSpVv[3Srb36gZZN{[Xl? MkLXTY5pcWKrdHnvckBw\iCQb4TjbEB{cWewYXzpcocheGG2aIfhfUBqdiCqdX3hckBJ\UyjIHPlcIx{KGG|c3Xzd4VlKGG|IH3lZZN2emWmIHHzJIxwf2W|dDDjc45k\W62cnH0bY9vKHKncYXpdoVlKDVyIITvJFYxKCViaX7obYJqfGmxbjDv[kBRVUFiaX7keYNm\CCudXPp[oVz[XOnIITy[YF1\WRiM{CgcYlveyCkZX\vdoUhcW6mdXP0bY9vNCCPRVS9OFDPxE1w MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV2N{i0N{c,OjN3NEe4OFM9N2F-
HeLa M2jORWZ2dmO2aX;uJIF{e2G7 MlmxTY5pcWKrdHnvckBw\iCQb4TjbEB{cWewYXzpcocheGG2aIfhfUBqdiCqdX3hckBJ\UyjIHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhWE2DIHnu[JVk\WRibIXjbYZmemG|ZTD0doVifGWmIEOwJI1qdnNiYnXmc5JmKGmwZIXjeIlwdixiSVO1NF01OM7:TT6= M4nzdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUS3PFQ{Lz5{M{W0O|g1OzxxYU6=
SK-MEL M2XsV2N6fG:2b4jpZ4l1gSCjc4PhfS=> M1HNZ|Q5KGi{cx?= MkXTR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2suVUWOIHPlcIx{KGG|c3Xzd4VlKGG|IHfyc5d1cCCrbnjpZol1cW:wIH3lZZN2emWmIHHmeIVzKDR6IHjyd{BjgSC[VGSgZZN{[XluIFnDOVA:PDdwM988UU4> M{O0VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUS3PFQ{Lz5{M{W0O|g1OzxxYU6=
HL60 MYDDfZRwfG:6aXPpeJkh[XO|YYm= NYC0bFZJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUEx4MDDj[YxteyxiRVS1NF0zOs7:TT6= M3;EelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUiyO|c5Lz5{M{W4Nlc4QDxxYU6=
Huh7.5.1 M4jhSmN6fG:2b4jpZ4l1gSCjc4PhfS=> NHnYcmw4OiCqcoO= MkXpR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVpPy53LkGgZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUGwMlbPxE1w MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ5M{KyOUc,OjN4N{OyNlU9N2F-
MCF7 MUfDfZRwfG:6aXPpeJkh[XO|YYm= MX23NkBpenN? MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDld5Rzd2enbjDy[YNmeHSxcjDwc5NqfGm4ZTDNR2Y4KGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MkiuNFfPxE1w NX3pZlRXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OlA2QTBpPkKzPFYxPTlyPD;hQi=>
SKBR3 M3jzOmN6fG:2b4jpZ4l1gSCjc4PhfS=> MVq3NkBpenN? Mo[3R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6g[ZN1em:pZX6gdoVk\XC2b4KgcoVo[XSrdnWgV2tDWjNiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME20NU41Os7:TT6= NXHkNZU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO4OlA2QTBpPkKzPFYxPTlyPD;hQi=>
ScN2a-cl3 NX3wUY9rTnWwY4Tpc44h[XO|YYm= NYG4fGR[PSCmYYnz NWfMPFZKUW6qaXLpeIlwdiCxZjDSUWwheHKrb36gdJJwfGWrbjDpcoZm[3SnZDDpckBud3W|ZTDkbZZq\GmwZzDTZ24z[S2lbEOgZ4VtdHNiZYjwdoV{e2mwZzDmeYxtKGynbnf0bEBud3W|ZTDQdnAh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJHBzWHOlIHzleoVtKGGodHXyJFUh\GG7czDifUBY\XO2ZYLuJIJtd3S2aX7nJIFv[Wy7c3nzMEBGSzVyPUCuOVjPxE1w MlmzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOEO1PFkoRjJ2MUizOVg6RC:jPh?=
ScN2a-cl3 MWrGeY5kfGmxbjDhd5NigQ>? NXm3O5BkPSCmYYnz NX\WN3VkUW6qaXLpeIlwdiCxZjDSUWwheHKrb36gdJJwfGWrbjDpcoZm[3SnZDDpckBud3W|ZTDkbZZq\GmwZzDTZ24z[S2lbEOgZ4VtdHNiZYjwdoV{e2mwZzDmeYxtKGynbnf0bEBud3W|ZTDQdnAh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIH;mJHBzWHOlIHzleoVtKGGodHXyJFUh\GG7czDifUBGVEmVQTygSWM2OD1|LkC2{txONg>? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF6M{W4PUc,OjRzOEO1PFk9N2F-
SH-SY5Y MmO5UoV2em:ycn;0[YN1cX[nIHHzd4F6 NVfJdXFpOTBidV2= MlL1NlQhcHK| NWT2cWtDVmW3cn;wdo91\WO2aY\lJIFkfGm4aYT5JIlvKGi3bXHuJHNJNVO\NWmgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBJOk9{LXnu[JVk\WRicnXkeYN1cW:wIH;mJINmdGxidnnhZoltcXS7IHH0JFExKHWPIHHmeIVzKDJ2IHjyd{BjgSCyaHHz[U1kd262cnHzeEBucWO{b3fyZZBpKGGwYXz5d4l{ NFXXXIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2PVUyPSd-MkSyOlk2OTV:L3G+
HEK293 NWHMTlQ5TnWwY4Tpc44h[XO|YYm= NVziNXhiPiCqcoO= NV;PeXR[UW6qaXLpeIlwdiCxZjDUUmYu[WyyaHGtbY5lfWOnZDDOSk1s[XCyYVKgZYN1cX[rdImgbY4hUEWNMkmzJINmdGy|IHHmeIVzKDZiaILzJIJ6KGy3Y3nm[ZJie2VicnXwc5J1\XJiZ3Xu[UBie3OjeTygTWM2OD1{LkZOwG0v MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDl7MkewNkc,OjR7OUK3NFI9N2F-
Hepa-1c1c7 NYr4XGJPTnWwY4Tpc44h[XO|YYm= MVi0PEBpenN? NGPad2ZKdmS3Y4Tpc44hd2ZicYXpco9v\SC{ZXT1Z5Rie2ViMTDpckBud3W|ZTDI[ZBiNTGlMXO3JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFPEQVIy|ryPLh?= M{fXUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2OUmyO|AzLz5{NEm5NlcxOjxxYU6=
HeLa MlX4SpVv[3Srb36gZZN{[Xl? M1ewV|ExKHSxIEGwNEB2VQ>? M3\YflI1KGi{cx?= MoHaTY5lfWO2aX;uJI9nKFOYNECgdJJwdW:2ZYKtcYVlcWG2ZXSgcJVkcW[ncnHz[UBo\W6nIHX4dJJme3Orb36gbY4hcHWvYX6gTIVN[SClZXzsd{BifCBzMDD0c{AyODBidV2gZYZ1\XJiMkSgbJJ{KGK7IGLUMXBEWiCjbnHsfZNqew>? NGfNfZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUG0O|YxPCd-MkWxOFc3ODR:L3G+
COLO205 M2P6[mFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M1\Jd2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ1;MU|IxPSClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:OjNwNd88UU4> NV\jTWVPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OVU1QDZpPkK1OFU2PDh4PD;hQi=>
MGC803 NHz2V29CdnSrcILvcIln\XKjdHn2[UBie3OjeR?= NEXsbXNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3HR|gxOyClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:PDMQvF2u NWHXVGFIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OVU1QDZpPkK1OFU2PDh4PD;hQi=>
MDA468 NH[4R3ZCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4P2XWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVTBOFY5KGOnbHzzJIJ6KE2WVDDhd5NigSxiSVO1NF01PS5{zszNMi=> NYrMTIZnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OVU1QDZpPkK1OFU2PDh4PD;hQi=>
HeLa NHnL[JVCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M4j1ZlQ5KGi{cx?= NFrMZoRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMh[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD12Nz65{txONg>? Ml\iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MUe3O|IoRjJ3OEG3O|czRC:jPh?=
RAW264.7 Mn;TRY51cS2rbn\sZY1u[XSxcomgZZN{[Xl? NXTYfVd2OTBibV2= M2H3NlExKG2rboO= NGThV2ZCdnSrLXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJGlNPiCycn;keYN1cW:wIHH0JFExKG2PIIDy[U1qdmO3YnH0[YQh\m:{IEGwJI1qdnNiYnXmc5JmKEySUzDzeIlufWyjdHnvckBnd3JiMkSgbJJ{KGK7IFXMTXNCKG2ndHjv[C=> M1XZfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MES4O|g5Lz5{NkC0PFc5QDxxYU6=
RAW264.7 MmDtRY51cS2rbn\sZY1u[XSxcomgZZN{[Xl? NHf6dJMyOCCvTR?= NELzO3UyOCCvaX7z NE\HTJRCdnSrLXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBTSVd{NkSuO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJHRPTmGucHjhJJBzd2S3Y4Tpc44h[XRiMUCgcW0heHKnLXnuZ5Vj[XSnZDDmc5IhOTBibXnud{Bj\W[xcnWgUHBUKHO2aX31cIF1cW:wIH\vdkAzPCCqcoOgZpkhTUyLU1GgcYV1cG:m NUS0c4dyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wOFg4QDhpPkK2NFQ5Pzh6PD;hQi=>
HaCaT MX\GeY5kfGmxbjDhd5NigQ>? NUmyZXZ4OSC3TR?= NVO1VJBiOSCqch?= MX7BZ5RqfmG2aX;uJI9nKFOLUmSxJIlvKGi3bXHuJGhiS2GWIHPlcIx{KGG|c3Xzd4VlKGG|IFi0T|E3KGSnYXPleJlt[XSrb36gZZQhOSC3TTDh[pRmeiBzIHjyJIJ6KFenc4Tldo4h[myxdITpcochdWW2aH;k MlPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ4OEmzOVIoRjJ4Nki5N|UzRC:jPh?=
HEK293 NEHxdZpHfW6ldHnvckBie3OjeR?= MWrBcpRi\2:waYP0JIFkfGm4aYT5JIF1KHKjdDDUVnBCOSCneIDy[ZN{\WRiaX6gTGVMOjl|IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhSUmWQz3pcoR2[2WmIHnuZ5Jm[XOnIH;mJIlvfHKjY3XscJVt[XJiY3HsZ4l2dSCuZY\lcEwhUUN3ME2wMlc2|ryPLh?= NFTMXYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nke1NFI2QCd-Mk[3OVAzPTh:L3G+
HEK293 MoXTSpVv[3Srb36gZZN{[Xl? M1XMTGFod26rc4SgZYN1cX[rdImgZZQhemG2IGTSVGEyKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIlvfHKjY3XscJVt[XJiY3HsZ4l2dSCuZY\lcEBqdiCjYoPlcoNmKG:oIFHJWGMtKEmFNUC9NU41|ryPLh?= M37KfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4N{WwNlU5Lz5{Nke1NFI2QDxxYU6=
HEK293 MXvGeY5kfGmxbjDhd5NigQ>? M4fneWFod26rc4SgZYN1cX[rdImgZZQhemG2IGTSVGEyKGW6cILld5Nm\CCrbjDISWszQTNiY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIlvfHKjY3XscJVt[XJiY3HsZ4l2dSCuZY\lcEBqdiCycnXz[Y5k\SCxZjDBTXRENCCLQ{WwQVIvPzUQvF2u MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd3MEK1PEc,OjZ5NUCyOVg9N2F-
HEK293 NVKyNYNMTnWwY4Tpc44h[XO|YYm= MoD2RY51[WexbnnzeEBi[3Srdnn0fUBifCC{YYSgWHJRSTFiZYjwdoV{e2WmIHnuJGhGUzJ7MzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKGGubInsJIl{d3SqaX;jfYFv[XSnLXnu[JVk\WRiaX7jdoVie2Vib3[gbY51emGlZXzseYxieiClYXzjbZVuKGyndnXsMEBKSzVyPUG5MlnPxE1w MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjd3MEK1PEc,OjZ5NUCyOVg9N2F-
U373-MAGI NVjOOW9VSW62aY\pdoFtKGG|c3H5 MXK1NEB2VQ>? MlzDNkBpenN? NW\WdIluWG:2ZX70bYF1cW:wIH;mJFUuSXqjLVOtbY5lfWOnZDDhcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDWV3YuTyCyc3X1[I91gXCnZDDITXYuOSCQTEStN{Bqdm[nY4Tl[EBqdiCqdX3hckBWOzd|LV3BS2kh[2WubIOgZZN{\XO|ZXSgZZMhPS2DenGtR{BGSzVyIHH0JFUxKHWPIIDy[Ylv[3WkYYTl[EBnd3JiMjDodpMh\m:ubH;3[YQh[nliNT3BfoEuSyCjZHTpeIlwdiCob4KgNkBpenNiYX7kJJN2[nOncYXlcpQhfmm{YXygbY5n\WO2aX;uJI1m[XO3LDDFR|UxRTFyzszNMi=> NHPQV409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GxO|I3OCd-MkexNVczPjB:L3G+
U373-MAGI MYXBcpRqfmm{YXygZZN{[Xl? MXe1NEB2VQ>? MUm0JIhzew>? NVfXVI5pSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhXlOYLVegdJNmfWSxdInw[YQhUEmYLUGgUmw1NTNiaX7m[YN1\WRiaX6gbJVu[W5iVUO3N{1OSUeLIHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckB3cXKjbDDpcoZm[3Srdnn0fUBifCB3MDD1UUBqdmO3YnH0[YQh\m:{IESgbJJ{KHC{aX;yJJRwKH[rcnHsJIlv\mWldHnvckBu\WG|dYLl[EBifCB5MjDodpMheG:|dDDpcoZm[3Srb36gZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nz NWTCSlNTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI MX\GeY5kfGmxbjDhd5NigQ>? MV:yNFAhfU1? NEHRPGc3KGi{cx?= NE[4VZhT\WS3Y4Tpc44hcW5iZFfUVEBt\X[nbDDpckBpfW2jbjDVN|c{NU2DR1mgZ4VtdHNiYYSgNlAxKHWPIHHmeIVzKDZiaILzJIJ6KEyFLV3TM21UKGGwYXz5d4l{ MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI M161eWZ2dmO2aX;uJIF{e2G7 M3HhNFIxOCC3TR?= M3nQS|YhcHK| MmPZVoVlfWO2aX;uJIlvKGSWVGCgcIV3\WxiaX6gbJVu[W5iVUO3N{1OSUeLIHPlcIx{KGG2IEKwNEB2VSCjZoTldkA3KGi{czDifUBNSy2PUz;NV{BidmGueYPpdy=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI M3zJNWFvfGm4aYLhcEBie3OjeR?= MVSyOUB1dyBzMECgeW0> MmPMNkBpenN? MlvmVI91\W62aXH0bY9vKG:oIEWtRZpiNUNvaX7keYNm\CCjboTpeolz[WxiYXP0bZZqfHliYXfhbY5{fCCYU2[tS{Bxe2W3ZH;0fZBm\CCKSW[tNUBPVDRvMzDpcoZm[3SnZDDpckBpfW2jbjDVN|c{NU2DR1mgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKH[rcnHsJIlv\mWldHn2bZR6KGG2IEK1JJRwKDFyMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFIhcHK|IH\vcIxwf2WmIHL5JFUuSXqjLVOgZYRlcXSrb36g[o9zKDJiaILzJIFv\CC|dXLz[ZF2 NE[ye2s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{GxO|I3OCd-MkexNVczPjB:L3G+
U373-MAGI NXfITIF[SW62aY\pdoFtKGG|c3H5 M4Dy[FIhfG9iODDodpM> M2i0R2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHZUXi2JIIDz[ZVld3S7cHXkJGhKXi1zIF7MOE0{KGmwZnXjeIVlKGmwIHj1cYFvKFV|N{OtUWFIUSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6geolz[WxiaX7m[YN1cX[rdImgdJJmcW6ldXLheIVlKG[xcjCyJJRwKDhiaILzJIZwdGyxd3XkJIJ6KH[rcnHsJIlv\mWldHnvckBu\WG|dYLl[EBifCB5MjDodpMheG:|dDDpcoZm[3Srb36gZpkh\myxdzDjfZRwdWW2cnnjJIFv[Wy7c3nz M1H4Z|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI NXnybHNMTnWwY4Tpc44h[XO|YYm= MYiyNFAhfU1? Mnu3OkBpenN? MV;S[YR2[3Srb36gbY4h\EOWUDDs[ZZmdCCrbjDoeY1idiCXM{ezMW1CT0liY3XscJMh[XRiMkCwJJVOKGGodHXyJFYhcHK|IHL5JGxENU2VL13TJIFv[Wy7c3nz NV6zVnB2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI Mk\OSpVv[3Srb36gZZN{[Xl? M131d|UxKHWP MoTaNkBpenN? MYTS[YR2[3Srb36gbY4h\EOWUDDs[ZZmdCCrbjDoeY1idiCXM{ezMW1CT0liY3XscJMh[XRiNUCgeW0heHKnaX7jeYJifGWmIH\vdkAzKGi{czDmc4xtd3enZDDifUA2NWG8YT3DJIFl\Gm2aX;uJI1m[XO3cnXkJIFnfGW{IESgbJJ{KGK7IFzDMW1UN02VIHHuZYx6e2m| MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI MXrGeY5kfGmxbjDhd5NigQ>? NH7HZoczODBidV2= NGmwNlc3KGi{cx?= MkHnVoVlfWO2aX;uJIlvKGSDVGCgcIV3\WxiaX6gbJVu[W5iVUO3N{1OSUeLIHPlcIx{KGG2IEKwNEB2VSCjZoTldkA3KGi{czDifUBNSy2PUz;NV{BidmGueYPpdy=> M1LJcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI MV\GeY5kfGmxbjDhd5NigQ>? MXuyNFAhfU1? MX6yJIhzew>? MnvwVoVlfWO2aX;uJIlvKGSUR2WtWHAhdGW4ZXygbY4hcHWvYX6gWVM4Oy2PQVfJJINmdGy|IHH0JFIxOCC3TTDwdoVqdmO3YnH0[YQh\m:{IEKgbJJ{KG[xbHzve4VlKGK7IEWtZZpiNUNiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhPCCqcoOgZpkhVENvTWOvUXMh[W6jbInzbZM> MkThQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI NHnhUlJHfW6ldHnvckBie3OjeR?= NG[yT4g2OCC3TR?= MVmyJIhzew>? MXHJcoNz\WG|ZTDpckA2NWG8YT3kR3RRN2SFVGCgdoF1cW9iaX6gbJVu[W5iVUO3N{1OSUeLIHPlcIx{KGG2IEWwJJVOKHC{ZXnuZ5Vj[XSnZDDmc5IhOiCqcoOg[o9tdG:5ZXSgZpkhPS2jenGt[GMh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgOEBpenNiYomgUGMuVVNxTWOgZY5idHm|aYOgdoVt[XSrdnWgeI8hPS2jenGt[GM> NWW0cJhpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI MWrGeY5kfGmxbjDhd5NigQ>? M{D2dFIxOCC3TR?= MoHsNkBpenN? NX34VZo2WmWmdXP0bY9vKGmwIHTDWHAhdGW4ZXygbY4hcHWvYX6gWVM4Oy2PQVfJJINmdGy|IHH0JFIxOCC3TTDwdoVqdmO3YnH0[YQh\m:{IEKgbJJ{KG[xbHzve4VlKGK7IEWtZZpiNUNiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhPCCqcoOgZpkhVENvTWOvUXMh[W6jbInzbZMhemWuYYTpeoUhfG9iNT3hfoEuSw>? MlHGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdzMUeyOlAoRjJ5MUG3NlYxRC:jPh?=
U373-MAGI Mn7HRY51cX[rcnHsJIF{e2G7 MnH5OVAhfU1? NHf1NVczKHSxIEeyJIhzew>? NHPmSI9Rd3SnboTpZZRqd25ib3[gOU1CgmFvQz3pcoR2[2WmIHHueIl3cXKjbDDhZ5Rqfmm2eTDh[4FqdnO2IG\TWk1IKHC|ZYXkc5R6eGWmIFjJWk0yKE6OND2zJIlv\mWldHXkJIlvKGi3bXHuJHU{PzNvTVHHTUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iVUWt[4FoKGyndnXsJIF1KDVyIIXNJIFnfGW{IEKgeI8hPzJiaILzJIJ6KHGSQ2KgcYV1cG:m NXPufId5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI NUjoXZNMSW62aY\pdoFtKGG|c3H5 NVLBcWVIPTBidV2= M3jHUFYhcHK| M4D5SmFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHZUXi2JIIDz[ZVld3S7cHXkJGhKXi1zIF7MOE0{KGmwZnXjeIVlKGmwIHj1cYFvKFV|N{OtUWFIUSClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6g[4FoKGyndnXsJIF1KDVyIIXNJI1m[XO3cnXkJIF1KDZiaILzJJBwe3RiaX7m[YN1cW:wIHL5JJFRS1JibXX0bI9l MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI MnS2SpVv[3Srb36gZZN{[Xl? NVzjcVFDOjByIIXN MWWyJIhzew>? MXTJcoNz\WG|ZTDpckA2NWG8YT3kR3RRN2SFVGCgdoF1cW9iaX6gbJVu[W5iVUO3N{1OSUeLIHPlcIx{KGG2IEKwNEB2VSCycnXpcoN2[mG2ZXSg[o9zKDJiaILzJIZwdGyxd3XkJIJ6KDVvYYrhMYREKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFQhcHK|IHL5JGxENU2VL13TJIFv[Wy7c3nzJJJmdGG2aY\lJJRwKDVvYYrhMYRE M2\2elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI M1\6c2Z2dmO2aX;uJIF{e2G7 MnfGNlAxKHWP MX:yJIhzew>? MkKyVoVlfWO2aX;uJIlvKDVvYYrhMYREXFBibHX2[YwhcW5iaIXtZY4hXTN5Mz3NRWdKKGOnbHzzJIF1KDJyMDD1UUBxemWrbnP1ZoF1\WRiZn;yJFIhcHK|IH\vcIxwf2WmIHL5JFUu[XqjLVOgZYRlcXSrb36gcYVie3W{ZXSgZYZ1\XJiNDDodpMh[nliTFOtUXMwVVNiYX7hcJl{cXN? NWj1VXYyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI MWLBcpRqfmm{YXygZZN{[Xl? NFe5Snc2OCC3TR?= MXOyJJRwKDhiaILz MXXQc5RmdnSrYYTpc44hd2ZiNT3BfoEuSy2rbnT1Z4VlKGGwdHn2bZJidCCjY4Tpeol1gSCjZ3HpcpN1KF[VVj3HJJB{\XWmb4T5dIVlKEiLVj2xJG5NPC1|IHnu[oVkfGWmIHnuJIh2dWGwIGWzO|MuVUGJSTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hfmm{YXygbY5n\WO2aY\peJkh[XRiNUCgeW0heHKnaX7jeYJifGWmIH\vdkAzKHSxIEigbJJ{KG[xbHzve4VlKGK7IEWtRZpiNUNiYXTkbZRqd25iZn;yJFIhcHK|IHHu[EB{fWK|ZYH1[Y4> M33yRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI M2rZUmFvfGm4aYLhcEBie3OjeR?= MV[yOUB2VQ>? NVnlfXl4OiCqcoO= NFLU[Y1Rd3SnboTpZZRqd25ib3[gOU1CgmFvZFOtbY5lfWOnZDDhcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDWV3YuTyCyc3X1[I91gXCnZDDITXYuOSCQTEStN{Bqdm[nY4Tl[EBqdiCqdX3hckBWOzd|LV3BS2kh[2WubIOgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwII\pdoFtKGmwZnXjeIl3cXS7IHH0JFI2KHWPIIDy[Ylv[3WkYYTl[EBnd3JiMjDodpMh\m:ubH;3[YQh[nliNT3BfoEu\ENiYXTkbZRqd25iZn;yJFIhcHK|IHHu[EB{fWK|ZYH1[Y51KHZ? M3jD[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI M3KxSmZ2dmO2aX;uJIF{e2G7 NGXjR2M2OCC3TR?= NELQUYozKGi{cx?= M4GxTWlv[3KnYYPlJIlvKDVvYYrhMYREXFBxZFPUVEBz[XSrbzDpckBpfW2jbjDVN|c{NU2DR1mgZ4VtdHNiYYSgOVAhfU1icILlbY5kfWKjdHXkJIZweiB{IHjyd{Bnd2yub4fl[EBjgSB3LXH6ZU1EKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFQhcHK|IHL5JGxENU2VL13TJIFv[Wy7c3nzJJJmdGG2aY\lJJRwKDVvYYrhMWM> M3\2OlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI NXz4No86TnWwY4Tpc44h[XO|YYm= NYTjOGF2PTBidV2= NGD0VZczKGi{cx?= MnzVVoVlfWO2aX;uJIlvKGSFVGCgcIV3\WxiaX6gbJVu[W5iVUO3N{1OSUeLIHPlcIx{KGG2IEWwJJVOKHC{ZXnuZ5Vj[XSnZDDmc5IhOiCqcoOg[o9tdG:5ZXSgZpkhPS2jenGt[GMh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgOEBpenNiYomgUGMuVVNxTWOgZY5idHm|aYOgdoVt[XSrdnWgeI8hPS2jenGt[GM> M3;zflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
U373-MAGI NYLsSZpJTnWwY4Tpc44h[XO|YYm= NFHFSIEzODBidV2= NHPaWlczKGi{cx?= M{O1dHJm\HWldHnvckBqdiCmQ2TQJIxmfmWuIHnuJIh2dWGwIGWzO|MuVUGJSTDj[YxteyCjdDCyNFAhfU1icILlbY5kfWKjdHXkJIZweiB{IHjyd{Bnd2yub4fl[EBjgSB3LXH6ZU1lSyCjZHTpeIlwdiCvZXHzeZJm\CCjZoTldkA1KGi{czDifUBNSy2PUz;NV{BidmGueYPpd{Bz\WyjdHn2[UB1dyB3LXH6ZU1lSw>? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzFzN{K2NEc,OjdzMUeyOlA9N2F-
U373-MAGI Mn24RY51cX[rcnHsJIF{e2G7 MWO1NEB2VQ>? MnnnNkB1dyB5MjDodpM> MnPuVI91\W62aXH0bY9vKG:oIEWtRZpiNWSFLXnu[JVk\WRiYX70bZZqemGuIHHjeIl3cXS7IHHnZYlve3RiVmPWMWcheHOndXTveJlx\WRiSFnWMVEhVkx2LUOgbY5n\WO2ZXSgbY4hcHWvYX6gWVM4Oy2PQVfJJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCXNT3nZYchdGW4ZXygZZQhPTBidV2gZYZ1\XJiMjD0c{A4OiCqcoOgZpkheVCFUjDt[ZRpd2R? NYCze3FTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI NH7MXnJCdnSrdnnyZYwh[XO|YYm= NYnEdW5iPTBidV2= MW[yJJRwKDd{IHjydy=> MkjBVI91\W62aXH0bY9vKG:oIEWtRZpiNUNvaX7keYNm\CCjboTpeolz[WxiYXP0bZZqfHliYXfhbY5{fCCYU2[tS{Bxe2W3ZH;0fZBm\CCKSW[tNUBPVDRvMzDpcoZm[3SnZDDpckBpfW2jbjDVN|c{NU2DR1mgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGejZzDs[ZZmdCCjdDC1NEB2VSCjZoTldkAzKHSxIEeyJIhzeyCkeTDxVGNTKG2ndHjv[C=> NYDte49lRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI MlHURY51cX[rcnHsJIF{e2G7 MoLSOVAhfU1? NGH3PFUzKHSxIEeyJIhzew>? M3nsdHBwfGWwdHnheIlwdiCxZjC1MWF7[S2mQz3pcoR2[2WmIHHueIl3cXKjbDDhZ5Rqfmm2eTDh[4FqdnO2IG\TWk1IKHC|ZYXkc5R6eGWmIFjJWk0yKE6OND2zJIlv\mWldHXkJIlvKGi3bXHuJHU{PzNvTVHHTUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iZ3HnJIxmfmWuIHH0JFUxKHWPIHHmeIVzKDJidH:gO|IhcHK|IHL5JJFRS1JibXX0bI9l NVXwbHdCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkexNVczPjBpPkK3NVE4OjZyPD;hQi=>
U373-MAGI M{O5UWFvfGm4aYLhcEBie3OjeR?= M4TzXFUxKHWP NUXycWlmPiCqcoO= NH3YPFJCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBXW1ZvRzDwd4V2\G:2eYDl[EBJUVZvMTDOUFQuOyCrbn\lZ5Rm\CCrbjDoeY1idiCXM{ezMW1CT0liY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJHU2NWejZzDs[ZZmdCCjdDC1NEB2VSCvZXHzeZJm\CCjdDC2JIhzeyCyb4P0JIlv\mWldHnvckBjgSCzUFPSJI1mfGixZB?= M{DNTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MUG3NlYxLz5{N{GxO|I3ODxxYU6=
DH5aplha M3nRUmZ2dmO2aX;uJIF{e2G7 NXXKUGpiOiCvaX7z NXfHSpI3UW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBpfW2jbjDDXXAySTFiZYjwdoV{e2WmIHnuJGV{[2incnnjbIliKGOxbHmgSGg2[XCuaHGgZ4VtdHNiYYPz[ZN{\WRiYYOgU{1l\WW2aInsZZRqd25ib3[g[ZRpd3i7cnXzc5J2\mmwIHnuJJBz\XOnbnPlJI9nKE6DRGDIJI1m[XO3cnXkJIFnfGW{IEKgcYlveyCkeTDzdIVkfHKxZnz1c5JwdWW2cnnjJI1mfGixZDygTWM2OD1|NN88UU4> M13xVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Mk[1NlU6Lz5{N{K2OVI2QTxxYU6=
MDAH 2774 M{PNdWN6fG:2b4jpZ4l1gSCjc4PhfS=> Mo\PO|IhcHK| M2TKXmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSUhiMke3OEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDDSFUxRTF2LkdOwG0v NUfnfnRGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke1NVU{OTlpPkK3OVE2OzF7PD;hQi=>
T24 MYfDfZRwfG:6aXPpeJkh[XO|YYm= NIryPJA4OiCqcoO= MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDUNlQh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygR2Q2OD1{Mj63{txONg>? M{\CVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUG1N|E6Lz5{N{WxOVMyQTxxYU6=
MDAH 2774 NHzD[HhEgXSxdH;4bYNqfHliYYPzZZk> NXHDWJJJPDhiaILz MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGFJKDJ5N{SgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiQ1S1NF0zO87:TT6= NFe4d|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{WxOVMyQSd-Mke1NVU{OTl:L3G+
DU145 NFzYTYVEgXSxdH;4bYNqfHliYYPzZZk> M3;yTFczKGi{cx?= NFy2VYFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBFXTF2NTDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCFREWwQVI4NjoQvF2u M4GzS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5NUG1N|E6Lz5{N{WxOVMyQTxxYU6=
MHRF M2HZV2N6fG:2b4jpZ4l1gSCjc4PhfS=> M{fkS|czKGi{cx?= MVHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNTHJHKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGNFPTB;M{NOwG0v NVux[4lwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke1NVU{OTlpPkK3OVE2OzF7PD;hQi=>
MDAH 2774 NX\ZeYRYS3m2b4TvfIlkcXS7IHHzd4F6 NXTMdIRrOjRiaILz NEjNOFhEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEGKIEK3O|Qh[2WubIOgZYZ1\XJiMkSgbJJ{KGK7IF3UWEBie3OjeTygR2Q2OD1|Nj65{txONg>? NFS3WlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{WxOVMyQSd-Mke1NVU{OTl:L3G+
HCT116 M3G4cWN6fG:2b4jpZ4l1gSCjc4PhfS=> MVSxNFAhfU1? NWq1W4dLPDhiaILz Mn3KR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGG|c3Xzd4VlKGG|IHPlcIwh\3Kxd4ToJIlvcGmkaYTpc44h[XRiMUCwJJVOKGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZk> NFHYZlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{i4O|g1Oyd-Mke4PFc5PDN:L3G+
Bel7402 NWnPPGJ6S3m2b4TvfIlkcXS7IHHzd4F6 M1S4d|ExOCC3TR?= NGD3S|k1QCCqcoO= MoLkR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRoVtPzRyMjDj[YxteyCjc4Pld5Nm\CCjczDj[YxtKGe{b4f0bEBqdmirYnn0bY9vKGG2IEGwNEB2VSCjZoTldkA1QCCqcoOgZpkhVVSWIHHzd4F6 NInlRmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{i4O|g1Oyd-Mke4PFc5PDN:L3G+
HepG2 MYXGeY5kfGmxbjDhd5NigQ>? NWmxcYVHPSCqcoO= NHS0S|VC[3SrdnH0bY9vKG:oIF7y[lIhMHWwa37ve44hd3KrZ3nuLUBmgHC{ZYPz[YQhcW5iaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPSCqcoOgZpkhSVKHLXTybZZmdiCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO|YYmsJGVEPTB;NT60{txONg>? NHXvTYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEGyOlQ1OCd-MkixNlY1PDB:L3G+
HEK293 NUSzVlVmT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NUfw[JJKOiCmYYnz MX\Hdo94fGhiaX7obYJqfGmxbjDv[kBJTUt{OUOgZ4VtdHNiYX\0[ZIhOiCmYYnzJIJ6KE2WVDDhd5NigSxiSVO1NF0yP87:TT6= MorDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh2MEiyNlQoRjJ6NEC4NlI1RC:jPh?=
MCF7 M3PNbGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NIr6TFEzKGSjeYO= NUCxd4xCT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hVUOINzDj[YxteyCjZoTldkAzKGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVM1|ryPLh?= M{fZZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEC4NlI1Lz5{OESwPFIzPDxxYU6=
Ramos M{[1PWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NVXMPJdWOiCmYYnz NI\1RoVIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBT[W2xczDj[YxteyCjZoTldkAzKGSjeYOgZpkhVVSWIHHzd4F6NCCLQ{WwQVM4|ryPLh?= M4G3RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NEC4NlI1Lz5{OESwPFIzPDxxYU6=
ALL5 MlHtR5l1d3SxeHnjbZR6KGG|c3H5 MXGxNlAhcHK| M3HjZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGFNVDViY3XscJMh[W[2ZYKgNVIxKGi{czDifUBOXFRibXX0bI9lNCCLQ{WwQVExNjYQvF2u MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDR7OUezNkc,Ojh2OUm3N|I9N2F-
HEK293 M4DsdmZ2dmO2aX;uJIF{e2G7 MlHLTY5pcWKrdHnvckBw\iCqdX3hckBVWlCDMTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKEGLVFOtbY5lfWOnZDDjeZJz\W62IILld5BwdnOnIHL5JJdpd2ynIHPlcIwheGG2Y3igZ4xidXBibXX0bI9lNCCLQ{WwQVAvPzYQvF2u MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDV5Nk[xO{c,Ojh3N{[2NVc9N2F-
LN229 Mnv0R5l1d3SxeHnjbZR6KGG|c3H5 M{jr[FQ5KGi{cx?= NFe3fIxEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBNVjJ{OTDj[YxteyCjc4Pld5Nm\CCjczDk[YNz\WG|ZTDpckBk\WyuII\pZYJqdGm2eTDh[pRmeiB2ODDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTFzzszNMi=> M2TRbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkW0NlY2Lz5{OE[1OFI3PTxxYU6=
NCI-H460 NWTObJhxS3m2b4TvfIlkcXS7IHHzd4F6 Ml3TOFghcHK| M1O1ZWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG5EUS2KNE[wJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUNOwG0v NX32THYxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OVQzPjVpPkK4OlU1OjZ3PD;hQi=>
MML1 NYn3PVdMS3m2b4TvfIlkcXS7IHHzd4F6 M3PLd|Q5KGi{cx?= M3S2OGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1OVDFiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yOs7:TT6= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ3NEK2OUc,Ojh4NUSyOlU9N2F-
BxPC3 NWf2WoJGS3m2b4TvfIlkcXS7IHHzd4F6 MnnXOFghcHK| NX;Te4dXS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSniSQ{OgZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4h[2WubDD2bYFjcWyrdImgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zNN88UU4> MnTsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4NUSyOlUoRjJ6NkW0NlY2RC:jPh?=
SK-MEL-2 MXrDfZRwfG:6aXPpeJkh[XO|YYm= M{\he|Q5KGi{cx?= M3HoR2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMNU2HTD2yJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUVOwG0v MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ3NEK2OUc,Ojh4NUSyOlU9N2F-
MDA-MB-231 MUPDfZRwfG:6aXPpeJkh[XO|YYm= MYq0PEBpenN? MX;DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MUZOwG0v MonUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh4NUSyOlUoRjJ6NkW0NlY2RC:jPh?=
A549 MmLjR5l1d3SxeHnjbZR6KGG|c3H5 M1zpcFQ5KGi{cx?= M1rYO2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yPs7:TT6= M4HqfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6NkW0NlY2Lz5{OE[1OFI3PTxxYU6=
U87 MVLDfZRwfG:6aXPpeJkh[XO|YYm= MoXmOFghcHK| Mke0R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWVg4KGOnbHzzJIF{e2W|c3XkJIF{KGSnY4LlZZNmKGmwIHPlcIwhfmmjYnnsbZR6KGGodHXyJFQ5KGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlDPxE1w NF[5N4k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[1OFI3PSd-Mki2OVQzPjV:L3G+
T47D NYn2d5RiS3m2b4TvfIlkcXS7IHHzd4F6 Mn33OFghcHK| NHTifIpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBVPDeGIHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OjYQvF2u NETKRpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[1OFI3PSd-Mki2OVQzPjV:L3G+
HT-29 NHr6T2ZEgXSxdH;4bYNqfHliYYPzZZk> NYHTbnU1PDhiaILz MlXOR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTHQuOjliY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gZ4VtdCC4aXHibYxqfHliYX\0[ZIhPDhiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0zP87:TT6= NHHi[YQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OE[1OFI3PSd-Mki2OVQzPjV:L3G+
COLO201 MWrDfZRwfG:6aXPpeJkh[XO|YYm= MUi0PEBpenN? NHy1fWVEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBEV0yRMkCxJINmdGy|IHHzd4V{e2WmIHHzJIRm[3KnYYPlJIlvKGOnbHygeoli[mmuaYT5JIFnfGW{IES4JIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;M{hOwG0v MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDZ3NEK2OUc,Ojh4NUSyOlU9N2F-
PANC1 M2WwOGN6fG:2b4jpZ4l1gSCjc4PhfS=> NWKwS|NyPDhiaILz M4fUemN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHBCVkNzIHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJINmdGxidnnhZoltcXS7IHHmeIVzKDR6IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OzoQvF2u NVLGe4d{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki2OVQzPjVpPkK4OlU1OjZ3PD;hQi=>
A549 M{TUWGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWPQZZpIT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hSTV2OTDj[YxteyxiSVO1NF0{OM7:TT6= NVXMdFdiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki4NVQ{PzRpPkK4PFE1Ozd2PD;hQi=>
BV2 NETkPVhCdnSrbnX1do9qdm[uYX3tZZRwenliYYPzZZk> M4n5VFI1KGi{cx?= MUHBcpRqdmW3cn;pcoZt[W2vYYTvdpkh[WO2aY\peJkhcW5ibX;1d4UhSlZ{IHPlcIx{KGG|c3Xzd4VlKGG|IHnubIljcXSrb36gc4YhVFCVLXnu[JVk\WRiTl:gdJJw\HWldHnvckBi\nSncjCyOEBpenNiaX6gdJJme2WwY3Wgc4YhVFCVIHL5JGdzcWW|czDy[YFkfGmxbjDiZZNm\CCjc4PhfUwhUUN3ME2xPE43Ps7:TT6= M1jlWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUGxPFE4Lz5{OEmxNVgyPzxxYU6=
A375 NEPHTWhHfW6ldHnvckBie3OjeR?= Mmq2NlUhfU1? MYiyOEBpenN? MUPJcohq[mm2aX;uJI9nKGGucHjhMW1UUC2rbnT1Z4VlKFSUUD2xJIV5eHKnc4Ppc44hcW5iaIXtZY4hSTN5NTDj[YxteyCjdDCyOUB2VSCjZoTldkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IH3leIhw\A>? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF3MEOzN{c,OjlzNUCzN|M9N2F-
A375 MWTGeY5kfGmxbjDhd5NigQ>? MmDYNlUhfU1? M{HzV|I1KGi{cx?= NVPT[HFoUW6qaXLpeIlwdiCxZjDhcJBp[S2PU1itbY5lfWOnZDDUVnAuOiCneIDy[ZN{cW:wIHnuJIh2dWGwIFGzO|Uh[2WubIOgZZQhOjVidV2gZYZ1\XJiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBu\XSqb3S= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF3MEOzN{c,OjlzNUCzN|M9N2F-
A375 MnPQSpVv[3Srb36gZZN{[Xl? NWW0fIg{OjVidV2= NYfoT2NRPCCqcoO= M{fSVWlvcGmkaYTpc44hd2ZiYXzwbIEuVVOKLXnu[JVk\WRicnXhZ5RqfmVib4j5[4VvKHOyZXPp[ZMh\2WwZYLheIlwdiCrbjDoeY1idiCDM{e1JINmdGy|IHH0JFI2KHWPIHHmeIVzKDRiaILzJIJ6KESFRlTBJJN1[WmwaX7nMYJie2WmIH\seY9z\XOlZX7j[UBie3OjeR?= MkOxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjlzNUCzN|MoRjJ7MUWwN|M{RC:jPh?=
HEK293T MUDDfZRwfG:6aXPpeJkh[XO|YYm= NVrKSotPOjRiaILz NEnBZpVEgXSxdH;4bYNqfHliYXfhbY5{fCCKRVuyPVNVKGOnbHzzJIFnfGW{IEK0JIhzeyCkeTDNWHQh[XO|YYmsJGNEPTB;MkKuO|XPxE1w MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MkC3PUc,OjlzN{KwO|k9N2F-
3T3L1 MkjCSpVv[3Srb36gZZN{[Xl? NGTPU2EyKHSxIEGwJJVO NVH3ZoJ[PiCmYYnz MoO3TY5pcWKrdHnvckBw\iC2cnnncJlk\XKrZHWgZYNkfW23bHH0bY9vKGmwIH3veZNmKDOWM1yxJINmdGy|IHH0JFEhfG9iMUCgeW0h[W[2ZYKgOkBl[Xm|IHL5JG9TVyC|dHHpcolv\y2kYYPl[EBucWO{b4Pjc5Bq[yCvZYToc4Q> MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTF5MkC3PUc,OjlzN{KwO|k9N2F-
RAW264.7 NVLSdllGTnWwY4Tpc44h[XO|YYm= M1LobVExKHWP NHnOUXEzPCCqcoO= M2fCemlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgWG5HNWGucHjhJJBzd2S3Y4Tpc44hcW5icnH0JHJCXzJ4ND63JINmdGy|IHH0JFExKHWPIHHmeIVzKDJ2IHjyd{BjgSCHTFnTRS=> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTRyN{m0OEc,Ojl2MEe5OFQ9N2F-
RAW264.7 NYrne4hNTnWwY4Tpc44h[XO|YYm= MV:xNEB2VQ>? MWSyOEBpenN? MknxTY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDJUE03KHC{b3T1Z5Rqd25iaX6gdoF1KFKDV{K2OE44KGOnbHzzJIF1KDFyIIXNJIFnfGW{IEK0JIhzeyCkeTDFUGlUSQ>? NWfrOW1wRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0NFc6PDRpPkK5OFA4QTR2PD;hQi=>
SK-N-MC NYDVZXpxeUiWUzDhd5NigQ>? NVnYbHlyeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPLMW4uVUNiY3XscJM> M4DXXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
BT-37 NIPJ[ZByUFSVIHHzd4F6 NXvKeXV4eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BV2 NGryVldCdnSrbnX1do9qdm[uYX3tZZRwenliYYPzZZk> NVfzcJBYOjRiaILz M3vtc2FvfGmwZYXyc4lv\myjbX3heI9zgSCjY4Tpeol1gSCrbjDtc5V{\SCEVkKgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iCOUGOtbY5lfWOnZDDJUE0y[mW2YTDwdo9lfWO2aX;uJIFnfGW{IEK0JIhzeyCkeTDFUGlUSSxiSVO1NF05NjgQvF2u MoXVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2OUm0PFUoRjJ7NEm5OFg2RC:jPh?=
BV2 M2jzSGFvfGmwZYXyc4lv\myjbX3heI9zgSCjc4PhfS=> MlnzNlQhcHK| MmrNRY51cW6ndYLvbY5ndGGvbXH0c5J6KGGldHn2bZR6KGmwIH3veZNmKEKYMjDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIGTOSoFteGijIIDyc4R2[3Srb36gZYZ1\XJiMkSgbJJ{KGK7IFXMTXNCNCCLQ{WwQVkvPc7:TT6= NID6dZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUS5PVQ5PSd-Mkm0PVk1QDV:L3G+
BV2 NHT6N|BCdnSrbnX1do9qdm[uYX3tZZRwenliYYPzZZk> MX[zNEBucW6| Ml:wRY51cW6ndYLvbY5ndGGvbXH0c5J6KGGldHn2bZR6KGmwIH3veZNmKEKYMjDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44heHKndILlZZRm\CCob4KgN|AhdWmwczDmc4xtd3enZDDifUBNWFNic4TpcZVt[XSrb36gZY5lKG2nYYP1doVlKGGodHXyJFI1KGi{czDifUBIemmnc4OgZZN{[XluIFnDOVA:OTFwMd88UU4> NYLJbXJRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0PVk1QDVpPkK5OFk6PDh3PD;hQi=>
J774 MWXBcpRqcW6obHHtcYF1d3K7IHHzd4F6 NGG0Z2ozPCCqcoO= MkTZRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDKO|c1KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDMVHMucW6mdXPl[EBKVDZiZYjwdoV{e2mxbjDh[pRmeiB{NDDodpMh[nliRVzJV2EtKEmFNUC9Ok45|ryPLh?= NY[5fFVGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm3NlY3QDBpPkK5O|I3PjhyPD;hQi=>
J774 MmTCRY51cWmwZnzhcY1ifG:{eTDhd5NigQ>? MUSyOEBpenN? NUf5cVJpSW62aXnu[oxidW2jdH;yfUBi[3Srdnn0fUBqdiCvb4Xz[UBLPzd2IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBNWFNvaX7keYNm\CCwaYTybYMhd3irZHWgdJJw\HWldHnvckBi\nSncjCyOEBpenNiYomgS5Jq\XO|IHHzd4F6NCCLQ{WwQVEzNjF3zszNMi=> MkS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5Mk[2PFAoRjJ7N{K2OlgxRC:jPh?=
J774 MYjBcpRqcW6obHHtcYF1d3K7IHHzd4F6 MmK1NlQhcHK| Mn7QRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDKO|c1KGOnbHzzJIF{e2W|c3XkJIF{KHKnZIXjeIlwdiCrbjDMVHMucW6mdXPl[EBOS1BzIHX4dJJme3Orb36gZYZ1\XJiMkSgbJJ{KGK7IFXMTXNCNCCLQ{WwQVE{NjcQvF2u NIGz[FI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUeyOlY5OCd-Mkm3NlY3QDB:L3G+
RAW 264.7 M3HkbGZ2dmO2aX;uJIF{e2G7 Mnv1NlQhcHK| M2jQN2lvcGmkaYTpc44hd2ZiTl\rZZBx[UJiaX6gcY92e2ViUlHXJFI3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBNWFNiaX7keYNm\CCQTzDwdo9lfWO2aX;uJIFnfGW{IEK0JIhzeyCrbjDwdoV{\W6lZTDv[kBQSkiVIHL5JGdzcWW|czDhd5NigSxiSVO1NF0yOc7:TT6= MkHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByNUO3PFMoRjNyMEWzO|g{RC:jPh?=
RAW 264.7 M4LwNmZ2dmO2aX;uJIF{e2G7 Mn\vNlQhcHK| MWLJcohq[mm2aX;uJI9nKE6Ia3HwdIFDKGmwIH3veZNmKFKDVzCyOlQvPyClZXzsd{Bie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gUHBUKGmwZIXj[YQhVk9icILv[JVkfGmxbjDh[pRmeiB{NDDodpMh[nliR4Lp[ZN{KGG|c3H5MEBKSzVyPUGzMlHPxE1w NVHMd3JpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CwOVM4QDNpPkOwNFU{Pzh|PD;hQi=>
MCF7 M2HrbmFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M2W5OlczKGi{cx?= NGPHRmpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3DSlch[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1{Mj65{txONg>? MofrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzByNUO3PFMoRjNyMEWzO|g{RC:jPh?=
MCF7 M17BPGFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NVy5U4ZMPzJiaILz M3zUNGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB5MjDodpMhcW5icILld4Vv[2Vib3[gU2JJWyCkeTDNWHQh[XO|YYmsJGlEPTB;MkWuOO69VS5? NI\MVpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC1N|c5Oyd-M{CwOVM4QDN:L3G+
BV2 NWTke3p3SW62aX7leZJwcW6obHHtcYF1d3K7IHHzd4F6 MVixNEB1dyB|MDD1US=> MmPMNlQhcHK| NUC3VW9xSW62aX7leZJwcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIFLWNkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJG5QKHC{b3T1Z5Rqd25iYYSgNVAhfG9iM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDhd5NigQ>? NG\qNmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEC5OlY2OCd-M{CwPVY3PTB:L3G+
RAW264.7 NWjaXWFNSW62aXnu[oxidW2jdH;yfUBie3OjeR?= M1HB[FEhcHJ? MmfPRY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz;JSm5o[W2vYT3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44h[nlibXXhd5VzcW6pIF7PJIxmfmWuczDpcoN2[mG2ZXSge4l1cCCOUGOvTWZP\2GvbXGg[o9zKDFiaIKg[o9tdG:5ZXSgZpkh[2:vcH;1coQh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgOFghcHK|IHL5JGdzcWW|czDhd5NigSBqUo\iJF0hPTViKz:tJFIvNCCDY4Tpeol1gT12ND61{txONg>? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODF7M{m0NUc,OzBzOUO5OFE9N2F-
T cells NY\jeXM6SW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= NEiwbZA2OCC3TR?= MnX3NUBpeg>? MYTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IFPvck1CNWmwZIXj[YQhXCClZXzsd{Bqe2:uYYTl[EBnem:vIIPwcIVvd2O7dHXzJI9nKEN3Nz;CUFYhdW:3c3WgZZQhPTBidV2gbY5kfWKjdHXkJJdqfGhiQ3;uMWEh\m:{IEGgbJIh\m:ubH;3[YQh[nliY3;tdI92dmRiYXTkbZRqd25ibXXhd5Vz\WRiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigQ>? NEPRTHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEG5N|k1OSd-M{CxPVM6PDF:L3G+
B cells M4LYOWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 M4nCR|UxKHWP NXm1XZlWOSCqch?= Mn\KRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDMVHMucW6mdXPl[EBDKGOnbHzzJIl{d2yjdHXkJIZzd21ic4Ds[Y5w[3m2ZYOgc4YhSzV5L1LMOkBud3W|ZTDheEA2OCC3TTDpcoN2[mG2ZXSge4l1cCCOUGOg[o9zKDFiaIKg[o9tdG:5ZXSgZpkh[2:vcH;1coQh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfS=> MlfPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzBzOUO5OFEoRjNyMUmzPVQyRC:jPh?=
RAW264.7 MlK4TY1ufW6xbX;keYxifG:{eTDhd5NigQ>? MoS4OVAhfU1? MoryNUBpeg>? M2D4TmludXWwb33v[JVt[XSxcomgZYN1cX[rdImgbY4hVFCVLYP0bY12dGG2ZXSgcY92e2ViUlHXNlY1NjdiY3XscJMh[XO|ZYPz[YQh[XNiZHXjdoVie2ViaX6gR2Q5OCCneIDy[ZN{cW:wIHH0JFUxKHWPIHnuZ5Vj[XSnZDD3bZRpKEySUzDmc5IhOSCqcjDmc4xtd3enZDDifUBkd22yb4Xu[EBi\GSrdHnvckBu\WG|dYLl[EBi\nSncjCyOEBpenNiYomg[oxwfyCleYTvcYV1enl? NH7rTW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEG5N|k1OSd-M{CxPVM6PDF:L3G+
RAW264.7 NHTYOXFKdW23bn;tc4R2dGG2b4L5JIF{e2G7 NIS4UVY2OCC3TR?= M4jBW|EhcHJ? NGnoNZpKdW23bn;tc4R2dGG2b4L5JIFkfGm4aYT5JIlvKEySUz3zeIlufWyjdHXkJI1wfXOnIGLBW|I3PC55IHPlcIx{KGG|c3Xzd4VlKGG|IHTlZ5Jm[XOnIHnuJGNFQDZiZYjwdoV{e2mxbjDheEA2OCC3TTDpcoN2[mG2ZXSge4l1cCCOUGOg[o9zKDFiaIKg[o9tdG:5ZXSgZpkh[2:vcH;1coQh[WSmaYTpc44hdWWjc4Xy[YQh[W[2ZYKgNlQhcHK|IHL5JIZtd3diY4n0c41mfHK7 NWfmbmNzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CxPVM6PDFpPkOwNVk{QTRzPD;hQi=>
HBZY-1 MUnGeY5kfGmxbjDhd5NigQ>? MWO2JJVO NIL1OGhC[3SrdnH0bY9vKG:oIF7y[lIhcW5icnH0JGhDYllvMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBPWU9vMTDs[ZZmdCCjdDC2JJVOKGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NUjpdVVkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C0NFg4PDlpPkOwOFA5PzR7PD;hQi=>
HBZY-1 MoDwSpVv[3Srb36gZZN{[Xl? MXy2JJVO NV6yeFZXSWO2aY\heIlwdiCxZjDOdoYzKGmwIILheEBJSlq\LUGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hUE9vMTDs[ZZmdCCjdDC2JJVOKGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NXXjbHdPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C0NFg4PDlpPkOwOFA5PzR7PD;hQi=>
HBZY-1 MnXQSpVv[3Srb36gZZN{[Xl? M1XCOlYhfU1? NIm1Rm9C[3SrdnH0bY9vKG:oIF7y[lIhcW5icnH0JGhDYllvMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBIS0yPIHzleoVtKGG2IE[geW0h[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NX7NNYliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{C0NFg4PDlpPkOwOFA5PzR7PD;hQi=>
HBZY-1 M1HPTWZ2dmO2aX;uJIF{e2G7 M4XNVlYhfU1? M2Dyc2FkfGm4YYTpc44hd2ZiToLmNkBqdiC{YYSgTGJbYS1zIHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJGdEVENibHX2[Ywh[XRiNjD1UUBjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NIDpR2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MESwPFc1QSd-M{C0NFg4PDl:L3G+
HBZY-1 Mn\zSpVv[3Srb36gZZN{[Xl? NX\BWWJUPiC3TR?= Mn74NlQhcHK| NH72NXBC[3SrdnH0bY9vKG:oIF7y[lIhcW5icnH0JGhDYllvMTDj[YxteyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBPWU9vMTDtVm5CKGW6cILld5Nqd25iYYSgOkB2VSCjZoTldkAzPCCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nz MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODRyOEe0PUc,OzB2MEi3OFk9N2F-
HBZY-1 M3n1XGZ2dmO2aX;uJIF{e2G7 NFX4Z2I3KHWP Moi2NlQhcHK| NXHofW1zSWO2aY\heIlwdiCxZjDOdoYzKGmwIILheEBJSlq\LUGgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hUE9vMTDtVm5CKGW6cILld5Nqd25iYYSgOkB2VSCjZoTldkAzPCCqcoOgZpkhWlRvUFPSJIFv[Wy7c3nz MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODRyOEe0PUc,OzB2MEi3OFk9N2F-
HBZY-1 NXfUUYVbTnWwY4Tpc44h[XO|YYm= M3X6cVYhfU1? NHO1cHIzPCCqcoO= MnXxRYN1cX[jdHnvckBw\iCQcn[yJIlvKHKjdDDIRnp[NTFiY3XscJMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gS2NNVSCvUl7BJIV5eHKnc4Ppc44h[XRiNjD1UUBi\nSncjCyOEBpenNiYomgVnQuWEOUIHHuZYx6e2m| M1u3ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyNEC4O|Q6Lz5|MESwPFc1QTxxYU6=
HBZY-1 M{nKXWZ2dmO2aX;uJIF{e2G7 NYT6ToJIPiC3TR?= MofoNlQhcHK| M{D2UWFkfGm4YYTpc44hd2ZiToLmNkBqdiC{YYSgTGJbYS1zIHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJGdEVENibWLORUBmgHC{ZYPzbY9vKGG2IE[geW0h[W[2ZYKgNlQhcHK|IHL5JHJVNVCFUjDhcoFtgXOrcx?= NIjQZ|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MESwPFc1QSd-M{C0NFg4PDl:L3G+
BV2 NF\FSo1CdnSrbnX1do9qdm[uYX3tZZRwenliYYPzZZk> NHHqV3c{OCCvaX7z MoDaNlQhcHK| NYHVRlJSSW62aX7leZJwcW6obHHtcYF1d3K7IHHjeIl3cXS7IHnuJI1wfXOnIFLWNkBk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oIFzQV{1qdmS3Y3XkJI5qfHKrYzDvfIll\SCycn;keYN1cW:wIIDy[ZRz\WG2ZXSg[o9zKDNyIH3pcpMh\m:ubH;3[YQh[nliTGDTMZN1cW23bHH0bY9vKG[xcjCyOEBpenNiYomgS5Jq\XO|IHHzd4F6NCCLQ{WwQVEyNjIQvF2u NFPWS2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEe3NVYxOSd-M{C3O|E3ODF:L3G+
MCF7 NX7L[YF2SW62aX;4bYRidnRiYYPzZZk> NFSwbosyODBidV2= M1jKRlI1KGi{cx?= M4fLfWFvfGmxeHnkZY51KGGldHn2bZR6KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOg[IVkemWjc3WgbY4hemWjY4TpeoUhd3i7Z3XuJJNx\WOrZYOg[4Vv\XKjdHnvckBifCBzMECgeW0hcW6ldXLheIVlKG[xcjCyOEBpenNiYomgR4VtdFKRWDDndoVmdi:Kb3XjbJN1KDN|M{SyJJN1[WmwaX7nJIJie2WmIHHzd4F6 M3WwPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyOUKxO|U5Lz5|MEmyNVc2QDxxYU6=
RAW264.7 NHXVdnhCdnSraX7mcIFudWG2b4L5JIF{e2G7 MnfmNVAhfU1? M{XZSFEhcHJ? NHGw[GxCdnSraX7mcIFudWG2b4L5JIFkfGm4aYT5JIlvKG2xdYPlJHJCXzJ4ND63JINmdGy|IHHzd4V{e2WmIHHzJIlvcGmkaYTpc44hd2ZiTGDTMYlv\HWlZXSgUm8heHKxZIXjeIlwdiCjdDCxNEB2VSCycnX0doVifGWmIH\vdkAyKGi{IH\vcIxwf2WmIHL5JGxRWyC|dHnteYxifGmxbjDhcoQhdWWjc4Xy[YQh[W[2ZYKgNlQhcHK|IHL5JGdzcWW|czDy[YFo\W62IHLhd4VlKGG|c3H5 Mn60QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzFyN{e5PVcoRjNzMEe3PVk4RC:jPh?=
HBZY-1 MkXxSpVv[3Srb36gZZN{[Xl? NUizTYVESmmwZHnu[{Bi\m[rbnn0fUB1dyCNZXHwNUBqdiC{YYSgTGJbYS1zIHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBPemZ{IH71Z4xm[XJidILhcpNtd2OjdHnvckBjgSCvZXHzeZJqdmdiaX7jdoVie2ViaX6gdoF1cW9ib3[gcpVkdGWjcjD0c{BkgXSxc3;sbYMhVnKoMjDs[ZZmdCCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> NGPhN2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUOwNVU3PCd-M{GzNFE2PjR:L3G+
HBZY-1 MoWySpVv[3Srb36gZZN{[Xl? M1XHeWJqdmSrbnegZYZncW6rdImgeI8hU2WjcEGgbY4hemG2IFjCXnkuOSClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gT4VieDFxToLmNkB{cWewYXzpcoch[nlibXXhd5VzcW6pIHnuZ5Jm[XOnIHnuJG5SVy1zIIDyc5RmcW5iZYjwdoV{e2mxbjDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTNyMUW2OEc,OzF|MEG1OlQ9N2F-
HBZY-1 NWTmdHJwTnWwY4Tpc44h[XO|YYm= MYHCbY5lcW6pIHHm[olvcXS7IITvJGtm[XBzIHnuJJJifCCKQmrZMVEh[2WubIOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIFvlZZAyN06{ZkKgd4lodmGuaX7nJIJ6KG2nYYP1dolv\yCrbnPy[YF{\SCrbjDIU{0yKHC{b4TlbY4h\XiycnXzd4lwdiCkeTDX[ZN1\XKwIHLsc5Qh[W6jbInzbZM> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTNyMUW2OEc,OzF|MEG1OlQ9N2F-
HBZY-1 MVvGeY5kfGmxbjDhd5NigQ>? NWPUc|VoSmmwZHnu[{Bi\m[rbnn0fUB1dyCNZXHwNUBqdiC{YYSgTGJbYS1zIHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBM\WGyMT;OdoYzKHOrZ37hcIlv\yCkeTDt[YF{fXKrbnegbY5kemWjc3WgbY4hT0OOTTDwdo91\WmwIHX4dJJme3Orb36gZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| NEXoRmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUOwNVU3PCd-M{GzNFE2PjR:L3G+
HBZY-1 M2XkVGZ2dmO2aX;uJIF{e2G7 NU[yOVc4SmmwZHnu[{Bi\m[rbnn0fUB1dyCNZXHwNUBqdiC{YYSgTGJbYS1zIHPlcIx{KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBM\WGyMT;OdoYzKHOrZ37hcIlv\yCkeTDt[YF{fXKrbnegbY5kemWjc3WgbY4hT0OOQzDwdo91\WmwIHX4dJJme3Orb36gZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| MmnTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF|MEG1OlQoRjNzM{CxOVY1RC:jPh?=
HBZY-1 MoL5SpVv[3Srb36gZZN{[Xl? NVez[mVqPiC3TR?= M3fsSWJqdmSrbnegZYZncW6rdImgeI8hU2WjcEGgbY4hemG2IFjCXnkuOSClZXzsd{Bie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gT4VieDFxToLmNkB{cWewYXzpcoch[nlibXXhd5VzcW6pIHnuZ5Jm[XOnIHnuJG5SVy1zIH3SUmEh\XiycnXzd4lwdiCjdDC2JJVOKGK7IGLUMXBEWiCjbnHsfZNqew>? NIKzeXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUOwNVU3PCd-M{GzNFE2PjR:L3G+
HBZY-1 Mn3NSpVv[3Srb36gZZN{[Xl? Mlq3OkB2VQ>? MoO0Rolv\GmwZzDh[oZqdmm2eTD0c{BM\WGyMTDpckBz[XRiSFLaXU0yKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDL[YFxOS:Qcn[yJJNq\26jbHnu[{BjgSCvZXHzeZJqdmdiaX7jdoVie2ViaX6gTG8uOSCvUl7BJIV5eHKnc4Ppc44h[XRiNjD1UUBjgSCUVD3QR3Ih[W6jbInzbZM> MmKxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF|MEG1OlQoRjNzM{CxOVY1RC:jPh?=
HBZY-1 NWrYPY9STnWwY4Tpc44h[XO|YYm= Ml\0OkB2VQ>? Ml\qRolv\GmwZzDh[oZqdmm2eTD0c{BM\WGyMTDpckBz[XRiSFLaXU0yKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDL[YFxOS:Qcn[yJJNq\26jbHnu[{BjgSCvZXHzeZJqdmdiaX7jdoVie2ViaX6gS2NNVSCvUl7BJIV5eHKnc4Ppc44h[XRiNjD1UUBjgSCUVD3QR3Ih[W6jbInzbZM> MlTIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF|MEG1OlQoRjNzM{CxOVY1RC:jPh?=
HBZY-1 M4XjNmZ2dmO2aX;uJIF{e2G7 M2PLVlYhfU1? MkTURolv\GmwZzDh[oZqdmm2eTD0c{BM\WGyMTDpckBz[XRiSFLaXU0yKGOnbHzzJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDL[YFxOS:Qcn[yJJNq\26jbHnu[{BjgSCvZXHzeZJqdmdiaX7jdoVie2ViaX6gS2NNSyCvUl7BJIV5eHKnc4Ppc44h[XRiNjD1UUBjgSCUVD3QR3Ih[W6jbInzbZM> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTNyMUW2OEc,OzF|MEG1OlQ9N2F-
RAW264.7 NY\E[45DSW62aXnu[oxidW2jdH;yfUBie3OjeR?= M4ru[FIhcHK| Mnf5RY51cWmwZnzhcY1ifG:{eTDhZ5Rqfmm2eTDpckBud3W|ZTDSRXczPjRwNzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKEySUz;JSm5o[W2vYT3pcoR2[2WmIF7PJJBzd2S3Y4Tpc44h[nlibXXhd5VzcW6pIF7PJIxmfmWuIIDy[Ylv[3WkYYTl[EBnd3JiMjDodpMh[W6mIH\vcIxwf2WmIHL5JGxRWy:LRl7nZY1u[SCjZHTpeIlwdiCjbnSgcYVie3W{ZXSgZYZ1\XJiMkSgbJJ{KGK7IFfybYV{eyCjc4PhfUwhUUN3ME20OE4y|ryPLh?= M1rLOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzM{WwNVI4Lz5|MUO1NFEzPzxxYU6=
PMNL NYXyUWFxTnWwY4Tpc44h[XO|YYm= MYGxOUBucW6| NXX4XVMzUW6qaXLpeIlwdiCxZjC1MWxQYCCrbjDoeY1idiCSTV7MJINmdGy|IHHzd4V{e2WmIHHzJGEzOzF6Nz3zeIlufWyjdHXkJGxVSjRicILv[JVkfGmxbjDwdoVqdmO3YnH0[YQh\m:{IEG1JI1qdnNiZn;scI94\WRiYomgRVI{OTh5IHHk[Il1cW:wIHHu[EBu\WG|dYLl[EBi\nSncjCxNEBucW6|IHL5JGhRVENiYX7hcJl{cXNuIFnDOVA:PC57zszNMi=> NH3NN4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUO1NVM6PSd-M{GzOVE{QTV:L3G+
H9c2 NIHLZplCdnSrb4jp[IFvfCCjc4PhfS=> M4XJclgxKG6PIITvJFIhfU1? M{GySVE5KGi{cx?= MnrCRY51cW:6aXThcpQh[WO2aY\peJkhcW5icnH0JGg6[zJiY3XscJMh[XO|ZYPz[YQh[XNicnXkeYN1cW:wIHnuJIh6eG:6aXGtbY5lfWOnZDDOU{Bz\WynYYPlJIF1KDhyIH7NJJRwKDJidV2gdJJmcW6ldXLheIVlKG[xcjCxPEBpenNiZn;scI94\WRiYomgbY5kfWKjdHXkJIlvKGi7cH;4bYEh\m:{IES4JIhzeyCkeTDndolme3NiYYPzZZk> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTN3MUO5OUc,OzF|NUGzPVU9N2F-
H9c2 M2O2T2FvfGmxeHnkZY51KGG|c3H5 M17iUFIhfU1? MWGxPEBpenN? MYXBcpRqd3irZHHueEBi[3Srdnn0fUBqdiC{YYSgTFlkOiClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDofZBwgGmjLXnu[JVk\WRicnXkeYN1cW:wIH;mJG1vW0:GIHX4dJJme3Orb36gZZQhOiC3TTDwdoVqdmO3YnH0[YQh\m:{IEG4JIhzeyCob3zsc5dm\CCkeTDpcoN2[mG2ZXSgbY4hcHmyb4jpZUBnd3JiNEigbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTN3MUO5OUc,OzF|NUGzPVU9N2F-
TOV112D NIrQOHhEgXSxdH;4bYNqfHliYYPzZZk> MXS5OkBpenN? MnzZR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWG9XOTF{RDDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKGWvcIT5JJZm[3SxcjDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkhcW6ldXLheIVlKG[xcjC5OkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGwMlY4|ryPLh?= NXfLVXJURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1NVQxOThpPkOxOVE1ODF6PD;hQi=>
TOV112D M4XEfmN6fG:2b4jpZ4l1gSCjc4PhfS=> M17a[Vk3KGi{cx?= M1rC[2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHRQXjFzMlSgZ4VtdHNiZYjwdoV{e2mwZzDOVW8zKGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDpcoN2[mG2ZXSg[o9zKDl4IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OTIQvF2u M2nNZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUG0NFE5Lz5|MUWxOFAyQDxxYU6=
SKOV3 M3myNmN6fG:2b4jpZ4l1gSCjc4PhfS=> NHrqcmg6PiCqcoO= NGO1fGpEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0:YMzDj[YxteyC2cnHud4Zm[3SnZDD3bZRpKG6xbj30ZZJo\XSnZDD2[YN1d3JiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOnbHygeoli[mmuaYT5JIlv[3WkYYTl[EBnd3JiOU[gbJJ{KGmwIIDy[ZNmdmOnIH;mJIRwgHmleXPsbY5mKGK7IF3UWEBie3OjeTygTWM2OD1{Od88UU4> NUnrSGRNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1NVQxOThpPkOxOVE1ODF6PD;hQi=>
SKOV3 M4nsSmN6fG:2b4jpZ4l1gSCjc4PhfS=> NIPIXmg6PiCqcoO= NEjyTI9EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0:YMzDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgbY5kfWKjdHXkJIZweiB7NjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTN4LkROwG0v Mnf5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3MUSwNVgoRjNzNUG0NFE5RC:jPh?=
SKOV3 M2X1OWN6fG:2b4jpZ4l1gSCjc4PhfS=> NHTPZ4E6PiCqcoO= M{X1O2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMV1Z|IHPlcIx{KHS{YX7z[oVkfGWmIIfpeIghe2iUTlGgZZN{\XO|ZXSgZZMhemWmdXP0bY9vKGmwIHPlcIwhfmmjYnnsbZR6KGmwY4XiZZRm\CCob4KgPVYhcHK|IHnuJJBz\XOnbnPlJI9nKGSxeInjfYNtcW6nIHL5JG1VXCCjc4PhfUwhUUN3ME2zPU43P87:TT6= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTVzNECxPEc,OzF3MUSwNVg9N2F-
HuH7 M4\we2Fxd3C2b4Ppd{Bie3OjeR?= NHe5PVIyODBidV2= NUDWbnpHPDhiaILz NGC4S3NKdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFj1TFch[2WubIOgeJJidnOoZXP0[YQhf2m2aDDTbHNIUzFiYYPz[ZN{\WRiYYOgcIF1\SCjcH;weI9{cXNiYYSgNVAxKHWPIHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KGGwbnX4bY4hXiCjbnSgO{1CSURic4ThbY5qdmdiYnHz[YQh[XO|YYm= Mmn2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3M{m3O|koRjNzNUO5O|c6RC:jPh?=
HUH7 NIn2O2dHfW6ldHnvckBie3OjeR?= NIDoVHEyODBidV2= NF3SRow3KGi{cx?= MXTJcohq[mm2aX;uJI9nKGmwc4XsbY4ucW6mdXPl[EBUT0tzIHnuJIh2dWGwIFjVTFch[2WubIOgZZQhOTByIIXNJJV{cW6pIIDldJRq\GViYYOgd5Vje3S{YYTlJIlv[3WkYYTl[EBnd3JiNjDodpMh[nlic3PpcpRqdGyjdHnvckBkd3WwdHXyJI1mfGixZB?= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV|OUe3PUc,OzF3M{m3O|k9N2F-
HuH7 MnX1SpVv[3Srb36gZZN{[Xl? NXvyV4pPOTByIIXN MWW2JIhzew>? M1LyPWlvcGmkaYTpc44hd2ZiU1fLNUBqdiCqdX3hckBJfUh5IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBqdnO3bHnuMYlv\HWlZXSgUWROOiCyaH;zdIhwenmuYYTpc44hd25iU3XyNVY3KGG2IEGwNEB2VSCrbnP1ZoF1\WRiZn;yJFYhcHK|IHL5JHdme3Sncn6gZoxwfCCjbnHsfZNqew>? M3jFdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUO5O|c6Lz5|MUWzPVc4QTxxYU6=
HuH7 NI\IeHlCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= M2XCNVExOCC3TR?= M3W3WVQ5KGi{cx?= M2LxbmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSIXIO{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iY3XscEB3cWGkaXzpeJkh[XRiMUCwJJVOKGmwY4XiZZRm\CCob4KgOFghcHK|IHL5JJRzgXCjbjDicJVmKGS7ZTDiZZNm\CCjc4PhfS=> MnvsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3M{m3O|koRjNzNUO5O|c6RC:jPh?=
HuH7 MnviRZBweHSxc3nzJIF{e2G7 NHW2TFEyODBidV2= Ml2wOFghcHK| MYrJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEi3SEegZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4h\WG{bImgZZBweHSxdHnjJINmdGy|IHH0JFExOCC3TTDpcoN2[mG2ZXSg[o9zKDR6IHjyd{BjgSCjbn7lfIlvKFZiYX7kJFcuSUGGIIP0ZYlvcW6pIHLhd4VlKGG|c3H5 NU\mSnhpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1N|k4PzlpPkOxOVM6Pzd7PD;hQi=>
HuH7 NXWxPYhHSXCxcITvd4l{KGG|c3H5 MlywNVAxKHWP MoG4OFghcHK| MkL1TY5lfWO2aX;uJI9nKGGyb4D0c5NqeyCrbjDoeY1idiCKdVi3JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGyjdHWgZZBweHSxdHnjJINmdGy|IHH0JFExOCC3TTDpcoN2[mG2ZXSg[o9zKDR6IHjyd{BjgSCjbn7lfIlvKFZiYX7kJFcuSUGGIIP0ZYlvcW6pIHLhd4VlKGG|c3H5 MkizQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzF3M{m3O|koRjNzNUO5O|c6RC:jPh?=
HuH7 NYnQZ3pYSXCxcITvd4l{KGG|c3H5 NXvlZXcyOTByIIXN M3PaNlQ5KGi{cx?= M{XL[Wlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iSIXIO{Bk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCwZXPyc5Rq[yClZXzsd{BifCBzMECgeW0hcW6ldXLheIVlKG[xcjC0PEBpenNiYomgZY5v\XirbjDWJIFv\CB5LVHBSEB{fGGrbnnu[{Bj[XOnZDDhd5NigQ>? NFP6WZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MUWzPVc4QSd-M{G1N|k4Pzl:L3G+
HuH7 M4fqV2FvfGmycn;sbYZmemG2aY\lJIF{e2G7 NU\mN25TOTByIIXN MmntOFghcHK| M2jpbmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSIXIO{Bk\WyuczD0doFve2[nY4Tl[EB4cXSqIGPoV2dMOSCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4h[2WubDD2bYFjcWyrdImgZZQhOTByIIXNJIlv[3WkYYTl[EBnd3JiNEigbJJ{KGK7IITyfZBidiCkbIXlJIR6\SCkYYPl[EBie3OjeR?= M3rZXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUO5O|c6Lz5|MUWzPVc4QTxxYU6=
HuH7 NUT6[4xTSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= MVOxNFAhfU1? MnvHOFghcHK| NFi5TI5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFj1TFch[2WubIOgc5ZmemW6cILld5NqdmdiRVfGVEBie3Onc4Pl[EBieyC{ZXT1Z5Rqd25iaX6gZ4VtdCC4aXHibYxqfHliYYSgNVAxKHWPIHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KHS{eYDhckBjdHWnIHT5[UBj[XOnZDDhd5NigQ>? M3XpNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNzNUO5O|c6Lz5|MUWzPVc4QTxxYU6=
HuH7 MY\BdI9xfG:|aYOgZZN{[Xl? Mnq1NVAxKHWP MXG0PEBpenN? M1m2cGlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iSIXIO{Bk\WyuczD0doFve2[nY4Tl[EB4cXSqIGPoV2dMOSCjc4Pld5Nm\CCjczDlZZJtgSCjcH;weI9{cXNiYYSgNVAxKHWPIHnuZ5Vj[XSnZDDmc5IhPDhiaILzJIJ6KGGwbnX4bY4hXiCjbnSgO{1CSURic4ThbY5qdmdiYnHz[YQh[XO|YYm= MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTV|OUe3PUc,OzF3M{m3O|k9N2F-
HuH7 MnzCRZBweHSxc3nzJIF{e2G7 MX[xNFAhfU1? Ml62OFghcHK| NWTLbHJIUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDIeWg4KGOnbHzzJI93\XKneIDy[ZN{cW6pIFXHSnAh[XRiMUCwJJVOKGmwY4XiZZRm\CCob4KgOFghcHK|IHL5JIFvdmW6aX6gWkBidmRiNz3BRWQhe3SjaX7pcoch[mG|ZXSgZZN{[Xl? NYe2R|Y1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{G1N|k4PzlpPkOxOVM6Pzd7PD;hQi=>
Assay
Methods Test Index PMID
Western blot Nanog / Oct-4 / Sox-2 p-EGFR / EGFR / p-HER2 / HER2 / p-ERK / ERK / PTEN 21304978 20729295
Growth inhibition assay Cell viability 23272133
体内研究 Resveratrol改善高热量饮食小鼠的健康和生存。Resveratrol (22.4 毫克/千克/天)使高热量饮食中年小鼠的生理机能转变为与标准饮食小鼠相符,并且显著增加他们的存活率。Resveratrol产生与延长寿命相关的改变,包括增加胰岛素敏感性,降低胰岛素样生长因子-1(IGF-I)的水平,增加AMP活化的蛋白激酶(AMPK) 过氧化物酶体增殖物激活的受体-γ共激活因子1α(PGC-1α)的活性,增加线粒体数量,并改善运动机能。在153显著改变的通路中,Resveratrol能够抵抗144的高热量饮食作用。[3] 在各种啮齿动物癌症模型中,Resveratrol被证明能够抑制肿瘤的发生和生长。Resveratrol在低至200微克/千克剂量下已经在结肠癌变的大鼠模型中表现出了功效。在40毫克/千克的较高剂量下,Resveratrol使患有皮下成神经细胞瘤的小鼠的存活率从0%增加到70%。Resveratrol在48微克/千克剂量下每天给药,抑制小鼠角膜微囊袋法中血管形成。[3] Resveratrol对心脏疾病表现出有益作用。Resveratrol阻断高脂饮食诱发的血小板聚集的增加。Resveratrol增加内皮细胞和诱导型一氧化氮合酶的表达。在易卒型自发性高血压大鼠体内,Resveratrol显著降低氧化应激的标志物,如血清中的糖化白蛋白和尿液中的8-羟基鸟嘌呤核苷。Resveratrol加入到饮用水中15天(1 毫克/千克)足以改善离体心脏的功能和冠状动脉血流量的恢复。[3] Resveratrol在体内显示抗炎活性。Resveratrol显著降低急慢性化学诱发的水肿,脂多糖诱发的气道炎症和骨关节炎,并且有助于防止同种异体移植排斥。在大鼠体内,Resveratrol静脉注射给药降低局部缺血/再灌注诱发的炎症,减少次黄嘌呤/黄嘌呤氧化酶(HX/ XO)或血小板活化因子产生的氧化剂,但不影响白三烯B4。[3] Resveratrol显示出对中风和脑损伤的有益作用。Resveratrol在100纳克/千克和1 微克/千克的极低剂量下静脉注射给药,显著减少大鼠大脑中动脉闭塞后缺血体积和脑组织含水量。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[6]

  • Cell lines: 人类乳腺癌细胞MCF-7
  • Concentrations: 30-300 μM
  • Incubation Time: 2天
  • Method:

    细胞接种于平底96孔微量滴定板(4000细胞/ 6.4毫米直径的孔)。在12-24小时后,细胞用DMSO(0.1–0.3%)处理或者增加Resveratrol的剂量。治疗48小时后,细胞用10μL 的MTT试剂在37℃下处理4小时,然后用100 μL增溶溶液在37 ℃下处理过夜。甲臜产生的数量利用分光光度微量滴定板读数器在570nm波长下测量。

动物实验:

[7]

  • Animal Models: 人类卵巢异种移植物PA-1
  • Dosages: 100毫克/千克
  • Administration: 每天腹腔注射连续四周

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+30% PEG 300+ddH2O

15mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 228.24
化学式

C14H12O3

CAS号 501-36-0
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03743636 Recruiting Drug: Nicotinamide riboside|Drug: Resveratrol|Other: Placebo Peripheral Artery Disease Northwestern University|American Heart Association October 1 2018 Phase 3

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

* 必填项

请输入您的姓名
请输入您的邮箱地址 请输入一个有效的邮箱地址
请写点东西给我们