Resveratrol (SRT501)

For research use only. Not for use in humans.

目录号:S1396 别名: trans-Resveratrol 中文名称:白藜芦醇

Resveratrol (SRT501) Chemical Structure

CAS No. 501-36-0

Resveratrol (SRT501, trans-Resveratrol)具有广泛靶点,包括环氧酶(如COX, IC50=1.1 μM)、脂肪氧合酶(LOC, IC50=2.7 μM)、sirtuins和其他蛋白质。它是一种天然的植物抗毒素,具有抗癌,抗炎,降血糖和其他有益心血管的作用。Resveratrol可诱导线粒体自噬、细胞自噬和自噬依赖性的凋亡。

规格 价格 库存 购买数量  
10mM (1mL in DMSO) RMB 1068.72 现货
RMB 804.89 现货
RMB 2551.88 现货
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客户使用Selleck生产的Resveratrol (SRT501)发表文献45篇:

产品安全说明书

Autophagy抑制剂选择性比较

生物活性

产品描述 Resveratrol (SRT501, trans-Resveratrol)具有广泛靶点,包括环氧酶(如COX, IC50=1.1 μM)、脂肪氧合酶(LOC, IC50=2.7 μM)、sirtuins和其他蛋白质。它是一种天然的植物抗毒素,具有抗癌,抗炎,降血糖和其他有益心血管的作用。Resveratrol可诱导线粒体自噬、细胞自噬和自噬依赖性的凋亡。
靶点
SIRT1 [2]
(Cell-free assay)
SIRT2 [2]
(Cell-free assay)
Quinone reductase 2 [9]
(Cell-free assay)
IKK β [1]
(Cell-free assay)
COX1 [9]
(Cell-free assay)
88 nM 1 μM 1.1 μM
体外研究

Resveratro 抑制环氧合酶和脂氧化酶,PKCs和p56lck, ERK1,JNK1,p38,IKK β,Src,STAT3,核苷酸还原酶,DNA聚合酶α和 δ,PKD,PKC α,醌还原酶2,以及芳香化酶的活性,IC50值为0.035-60 μM。Resveratrol也是腺苷酸环化酶和AMPK的活化剂,IC50值分别为0.8 μM和50 μM。Resveratrol作为抑制剂或活化剂,能够减少细胞炎症相关的表现,抑制癌症细胞的生长并诱导细胞凋亡,逆转内皮素-1受激的细胞应答,抑制佛波醇酯诱导的COX-2表达,抑制细胞中DNA合成,抵抗甲萘醌诱导的细胞死亡,并且改善细胞线粒体功能和葡萄糖/脂质代谢。[1]Resveratrol也是抗衰老酶的活化剂。Resveratrol降低作用于乙酰化底物和NAD+的SIRT1的Michaelis常数,通过促进SIRT1依赖性的p53脱乙酰作用增加细胞存活。在酵母中,Resveratrol通过刺激Sir2模拟热量限制,增加DNA稳定性并延长寿命。[2] Resveratrol通过其抗氧化活性,能够有效保护离体的大鼠心脏免受心脏缺血/再灌注损伤,改善发展压和主动脉血流的恢复,降低丙二醛的浓度并减少梗死面积。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HL60 cell line NVX4b5FHWHKxbHnm[ZJifGmxbjDhd5NigQ>? NFjYV2pCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjMOlAh[2WubDDsbY5mNCCLQ{WwQVUh|ryP M37HXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4Nki2OVQ{Lz5zNk[4OlU1OzxxYU6=
human K562 cell line M{iwO3Bzd2yrZnXyZZRqd25iYYPzZZk> NUD1VXh6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLOVYzKGOnbHygcIlv\SCneIDy[ZN{cW6pIFLjdk1C[mxuIFnDOVA:OjhizszN MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjZ6NkW0N{c,OTZ4OE[1OFM9N2F-
HUVEC M{DDcmZ2dmO2aX;uJIF{e2G7 M1SwdVExOCEQvF2= Mo\qNUBl[Xl? Mn3BTY5lfWO2aX;uJI9nKG2rdH;jbI9v\HKrYXygZolw\2WwZYPpd{BqdiCKMl:yMYlv\HWlZXSgTHVXTUNiYYSgNVAxKHWvb3yvUEBxemW2cnXheIVlKG[xcjCxJIRigSCob3zsc5dm\CCkeTDjc41xd3WwZDD3ZZNpd3W2IHHu[EBJOk9{LYTy[YF1dWWwdDDt[YF{fXKnZDDh[pRmeiBzMDDkZZl{KGK7IF3peI9VemGla3XyJHJm\CC|dHHpcolv\y2kYYPl[EBie3OjeR?= M1;yTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEW5NlQ6Lz5{M{i1PVI1QTxxYU6=
Hepa1clc7 MXPDfZRwfG:6aXPpeJkh[XO|YYm= M37XUlczKGi{cx?= MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBud3W|ZTDI[ZBiOWOuY{egZ4VtdHNiYX\0[ZIhPzJiaILzMEBKSzVyPUGx{txONg>? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODB5NUe0Nkc,OTByN{W3OFI9N2F-
MCF7 MlLiSpVv[3Srb36gZZN{[Xl? NFXBTG03KGi{cx?= MnjaTY5pcWKrdHnvckBw\iCyaH;yZo9tKGW|dHXyMYlv\HWlZXSgc5JvcXSqaX7lJIRm[2G{Yn;4fYxie2ViaX6gbJVu[W5iTVPGO{Bk\WyuczDh[pRmeiB4IHjyd{whUUN3ME2xPe69VS5? NIrHfG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMEC3OVc1Oid-MUCwO|U4PDJ:L3G+
U937 M4jVVGZ2dmO2aX;uJIF{e2G7 MYTJcohq[mm2aX;uJI9nKFtzNFPd[IVpgWS{b3HzZ49z[mmlIHHjbYQhfXC2YXvlJIlvKGi3bXHuJHU6OzdiY3XscJMh[nliTXnjbIFmdGm|LV3lcpRmdiCybH;0MEBMcT1|Nd88UU4> M4r1NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMke3O|Y1Lz5zMUK3O|c3PDxxYU6=
U937 MWLGeY5kfGmxbjDhd5NigQ>? M4PIcmlvcGmkaYTpc44hd2ZiMj3bNUwzNTOKKF6pYYRmd3i7LVSt[4x2[2:|ZTD1dJRic2ViaX6gbJVu[W5iVUmzO{Bk\WyuczDifUB{[2mwdHnscIF1cW:wIIPw[YN1em:vZYTyfUBqdiCycnXz[Y5k\SCxZjDzc4RqfW1vZoLl[UBjfW[oZYK= M1n6PFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMke3O|Y1Lz5zMUK3O|c3PDxxYU6=
HL60 NFH4bItHfW6ldHnvckBie3OjeR?= NIPGXo9KdmirYnn0bY9vKG:oIEKtX|EtOi1|SDjOLX1l\W:6eT3EMYdtfWOxc3WgeZB1[WunIHnuJIh2dWGwIFjMOlAh[2WubIOgZpkhe2OrboTpcIxifGmxbjDzdIVkfHKxbXX0dpkhcW5icILld4Vv[2Vib3[gd49lcXWvLX\y[YUh[nWoZnXy Mon6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{N{e3OlQoRjFzMke3O|Y1RC:jPh?=
U937 NVv0O5UxTnWwY4Tpc44h[XO|YYm= NFn1fWlKdmirYnn0bY9vKG:oIGuxOGNe\GWqeXTyc4F{[2:{YnnjJIFkcWRidYD0ZYtmKGmwIHj1cYFvKFV7M{egZ4VtdHNiYomgd4NqdnSrbHzheIlwdiC|cHXjeJJwdWW2comgbY4heHKnc3XuZ4Uhd2Zic3;kbZVuNW[{ZXWgZpVn\mW{ NYnmNlBLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUGyO|c4PjRpPkGxNlc4PzZ2PD;hQi=>
HL60 MkPhSpVv[3Srb36gZZN{[Xl? M2nPOmlvcGmkaYTpc44hd2ZiW{G0R31l\Wi7ZILvZZNkd3KkaXOgZYNq\CC3cIThb4UhcW5iaIXtZY4hUEx4MDDj[YxteyCkeTDzZ4lvfGmubHH0bY9vKHOyZXP0do9u\XS{eTDpckBxemW|ZX7j[UBw\iC|b3TpeY0u\nKnZTDieYZn\XJ? MlXlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF{N{e3OlQoRjFzMke3O|Y1RC:jPh?=
CHO NXGzVo03TnWwY4Tpc44h[XO|YYm= MoLZRoxw[2ujZHWgc4YhemG2IFfseZQyKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjCyMXsyNDJvM1ioUkle\GWxeImtSE1odHWlb4PlJJVxfGGtZTDifUB{[2mwdHnscIF1cW:wIIPw[YN1em:vZYTyfS=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTJ5N{e2OEc,OTF{N{e3OlQ9N2F-
CHO NWLnPWhVTnWwY4Tpc44h[XO|YYm= MmTtRoxw[2ujZHWgc4YhemG2IFfseZQyKGW6cILld5Nm\CCrbjDDTG8h[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjDbNVREZWSnaInkdo9ie2OxcnLpZ{Bi[2mmIIXweIFs\SCkeTDzZ4lvfGmubHH0bY9vKHOyZXP0do9u\XS{eR?= NXLJeIJTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUGyO|c4PjRpPkGxNlc4PzZ2PD;hQi=>
CHO NEjYVmtHfW6ldHnvckBie3OjeR?= NV7PXlRHSmyxY3vh[IUhd2ZiaIXtZY4hT2y3dEOg[ZhxemW|c3XkJIlvKEOKTzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKDJvW{GsNk0{UCiQKW3k[Y95gS2GLXfseYNwe2VidYD0ZYtmKGK7IIPjbY51cWyuYYTpc44he3CnY4Tyc41mfHK7 MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTJ5N{e2OEc,OTF{N{e3OlQ9N2F-
CHO NULieZkyTnWwY4Tpc44h[XO|YYm= MmjORoxw[2ujZHWgc4YhcHWvYX6gS4x2fDNiZYjwdoV{e2WmIHnuJGNJVyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJHsyPEOfZHXofYRzd2G|Y3;yZolkKGGlaXSgeZB1[WunIHL5JJNkcW62aXzsZZRqd25ic4DlZ5Rzd22ndIL5 NE\te3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUK3O|c3PCd-MUGyO|c4PjR:L3G+
HL60 NVXIVYlHTnWwY4Tpc44h[XO|YYm= NV30R|BuUW5idnn0do8hcW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgdJJwdGmoZYLheIlwdiCxZjDIUFYxKGOnbHzzMEBKSzVyPUZOwG0v M1\1VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{OEe3OVk{Lz5zMki3O|U6OzxxYU6=
RAW264.7 MmjNSpVv[3Srb36gZZN{[Xl? M3L1dmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBvcXS{aXOgc5hq\GVicILv[JVkfGmxbjDpckBtcXCxcH;sfZNi[2OqYYLp[IUu[WO2aY\heIVlKG2xdYPlJI1i[3KxcHjh[4UhWkGZMk[0Mlch[2WubIOsJGlEPTB;MUWuPe69VS5? MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV3Mke1PUc,OTR3NUK3OVk9N2F-
RAW264.7 M3rpV2Z2dmO2aX;uJIF{e2G7 M3r0emlvcGmkaYTvdpkh\W[oZXP0JI9vKFCJRT2yJJBzd2S3Y4Tpc44hcW5iTGDTMZN1cW23bHH0[YQhWkGZMk[0Mlch[2WubIOsJGlEPTB;NN88UU4> M3G2dFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MEiwPVg5Lz5zNUC4NFk5QDxxYU6=
RAW264.7 NGG5cnVHfW6ldHnvckBie3OjeR?= NWPTOIV{cW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgVGdGNTJicILv[JVkfGmxbjDpckBNWFNvc4TpcZVt[XSnZDDSRXczPjRwNzDj[YxteyxiSVO1NF0zOC56zszNMi=> Mn7FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV3MEGwOlQoRjF3NUCxNFY1RC:jPh?=
T47D MkXVSpVv[3Srb36gZZN{[Xl? NHXQSI9Ge3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKFR2N1SgZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW|aX7nJJN1cW23bHH0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbjDldZVqfmGuZX70JJRwKDFyIIDNJIV{fHKjZHnvcEBjgSCjbHHtZZIh[my3ZTDhd5NigSxiQXP0bZZqfHl;MD6wN:69VS5? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTd6N{SzOkc,OTV5OEe0N|Y9N2F-
MCF7 NFjkUHZHfW6ldHnvckBie3OjeR?= NHvpTlhGe3S{b3flcolkKGGldHn2bZR6KGmwIHj1cYFvKE2FRkegZ4VtdHNiYYPz[ZN{\WRiYYOg[JJ2\yCuZY\lcEBk[XW|aX7nJJN1cW23bHH0bY9vKG:oIHPlcIwheHKxbHnm[ZJifGmxbjDldZVqfmGuZX70JJRwKDFyIIDNJIV{fHKjZHnvcEBjgSCjbHHtZZIh[my3ZTDhd5NigSxiQXP0bZZqfHl;MT62{txONg>? M1HkO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{i3OFM3Lz5zNUe4O|Q{PjxxYU6=
MDA-MB-231 NV\we5dtSW62aYDyc4xq\mW{YYTpeoUh[XO|YYm= M3rreWlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDDmc5Ih[W62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvMkOxJINmdGy|LDDJR|UxRTJyLkZOwG0v NVXMWJFjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[yOVA3OzZpPkG2NlUxPjN4PD;hQi=>
U937 NUjJfXNMS3m2b4TvfIlkcXS7IHHzd4F6 NUKwXZN[S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTl|NzDj[YxteyCjZoTldkA1QCCqcoOgZpkhX1OWMTD0[ZN1NCCLQ{WwQVE4|ryPLh?= NF20OoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkK1NlkzPCd-MU[yOVI6OjR:L3G+
HL60 MUnBcpRqeHKxbHnm[ZJifGm4ZTDhd5NigQ>? MX7BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiONkCgZ4VtdHNuIFnDOVA:Pc7:TT6= M3vEeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4NUiwNlA1Lz5zNkW4NFIxPDxxYU6=
HEK293T NIDvb4JHfW6ldHnvckBie3OjeR?= MYHJcohq[mm2aX;uJI9nKFSQRj3hcJBp[S2rbnT1Z4VlKE6ILXvhdJBiSiCjY4TpeoF1cW:wIHnuJGhGUzJ7M2SgZ4VtdHNuIFnDOVA:OjEQvF2u MmX4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzMkWyO|AoRjF5MUK1NlcxRC:jPh?=
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RAW 264.7 MULGeY5kfGmxbjDhd5NigQ>? NULMeXNbUW6qaXLpeIlwdiCxZjDMVHMh[W6mIGTOSk1idHCqYT3hZ5RqfmG2ZXSgUm8heHKxZIXjeIlwdiCrbjDteZJqdmViUlHXJFI3PC55IHPlcIx{NCCLQ{WwQVI{NjYQvF2u MmXnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd{NUO4OFQoRjF5MkWzPFQ1RC:jPh?=
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... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Nanog / Oct-4 / Sox-2 ; 

PubMed: 21304978     


Pancreatic CSCs were treated with resveratrol (0–30 µM) for 48 h. The expression of Nanog, Oct-4 and Sox-2 was measured by the Western blot analysis. GAPDH was used as a loading control. 

p-EGFR / EGFR / p-HER2 / HER2 / p-ERK / ERK / PTEN ; 

PubMed: 20729295     


DU145 cells were treated with DMSO, and 10, 20 or 50 µm resveratrol. Western blots show resveratrol decreased phosphorylation of EGFR-Tyr1068 (upper panel), HER2-Tyr1221/1222 (second panel).

21304978 20729295
Growth inhibition assay
Cell viability; 

PubMed: 23272133     


A, MTS assay was performed on PC-3M-MM2 cells treated with vehicle or different concentrations of resveratrol (5–100 µM) for 72 h which showed that resveratrol dose-dependently reduced cell viability. B, Decrease in cell viability by resveratrol was time-dependent in the resveratrol-treated groups (25 and 50 µM) compared to vehicle.

23272133
体内研究 Resveratrol改善高热量饮食小鼠的健康和生存。Resveratrol (22.4 毫克/千克/天)使高热量饮食中年小鼠的生理机能转变为与标准饮食小鼠相符,并且显著增加他们的存活率。Resveratrol产生与延长寿命相关的改变,包括增加胰岛素敏感性,降低胰岛素样生长因子-1(IGF-I)的水平,增加AMP活化的蛋白激酶(AMPK) 过氧化物酶体增殖物激活的受体-γ共激活因子1α(PGC-1α)的活性,增加线粒体数量,并改善运动机能。在153显著改变的通路中,Resveratrol能够抵抗144的高热量饮食作用。[3] 在各种啮齿动物癌症模型中,Resveratrol被证明能够抑制肿瘤的发生和生长。Resveratrol在低至200微克/千克剂量下已经在结肠癌变的大鼠模型中表现出了功效。在40毫克/千克的较高剂量下,Resveratrol使患有皮下成神经细胞瘤的小鼠的存活率从0%增加到70%。Resveratrol在48微克/千克剂量下每天给药,抑制小鼠角膜微囊袋法中血管形成。[3] Resveratrol对心脏疾病表现出有益作用。Resveratrol阻断高脂饮食诱发的血小板聚集的增加。Resveratrol增加内皮细胞和诱导型一氧化氮合酶的表达。在易卒型自发性高血压大鼠体内,Resveratrol显著降低氧化应激的标志物,如血清中的糖化白蛋白和尿液中的8-羟基鸟嘌呤核苷。Resveratrol加入到饮用水中15天(1 毫克/千克)足以改善离体心脏的功能和冠状动脉血流量的恢复。[3] Resveratrol在体内显示抗炎活性。Resveratrol显著降低急慢性化学诱发的水肿,脂多糖诱发的气道炎症和骨关节炎,并且有助于防止同种异体移植排斥。在大鼠体内,Resveratrol静脉注射给药降低局部缺血/再灌注诱发的炎症,减少次黄嘌呤/黄嘌呤氧化酶(HX/ XO)或血小板活化因子产生的氧化剂,但不影响白三烯B4。[3] Resveratrol显示出对中风和脑损伤的有益作用。Resveratrol在100纳克/千克和1 微克/千克的极低剂量下静脉注射给药,显著减少大鼠大脑中动脉闭塞后缺血体积和脑组织含水量。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

细胞实验:

[6]

- 合并
  • Cell lines: 人类乳腺癌细胞MCF-7
  • Concentrations: 30-300 μM
  • Incubation Time: 2天
  • Method:

    细胞接种于平底96孔微量滴定板(4000细胞/ 6.4毫米直径的孔)。在12-24小时后,细胞用DMSO(0.1–0.3%)处理或者增加Resveratrol的剂量。治疗48小时后,细胞用10μL 的MTT试剂在37℃下处理4小时,然后用100 μL增溶溶液在37 ℃下处理过夜。甲臜产生的数量利用分光光度微量滴定板读数器在570nm波长下测量。


    (Only for Reference)
动物实验:

[7]

- 合并
  • Animal Models: 人类卵巢异种移植物PA-1
  • Dosages: 100毫克/千克
  • Administration: 每天腹腔注射连续四周
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 45 mg/mL warmed (197.16 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5% DMSO+30% PEG 300+ddH2O
15mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 228.24
化学式

C14H12O3

CAS号 501-36-0
储存条件 粉状
溶于溶剂
别名 trans-Resveratrol

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03743636 Recruiting Drug: Nicotinamide riboside|Drug: Resveratrol|Other: Placebo Peripheral Artery Disease Northwestern University|American Heart Association October 1 2018 Phase 3

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操作手册

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Autophagy Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID