Sorafenib (BAY 43-9006)

目录号:S7397 别名: NSC-724772 中文名称:索拉非尼

仅限科研使用

Sorafenib (BAY 43-9006, NSC-724772)是Raf-1B-Raf的多重激酶抑制剂,无细胞试验中IC50分别为6 nM和22 nM。Sorafenib 还可抑制VEGFR-2VEGFR-3PDGFR-βFlt-3c-KIT,对应的IC50值分别为90 nM、20 nM、57 nM、59 nM和68 nM。Sorafenib 可诱导autophagyapoptosis并激活ferroptosis,Sorafenib 具有抗肿瘤活性。

Sorafenib (BAY 43-9006) Chemical Structure

CAS: 284461-73-0

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 647.05 现货
RMB 573.93 现货
RMB 1040.95 现货
RMB 2597.13 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的Sorafenib (BAY 43-9006)发表文献437篇:

产品安全说明书

Raf抑制剂选择性比较

相关Raf产品

生物活性

产品描述 Sorafenib (BAY 43-9006, NSC-724772)是Raf-1B-Raf的多重激酶抑制剂,无细胞试验中IC50分别为6 nM和22 nM。Sorafenib 还可抑制VEGFR-2VEGFR-3PDGFR-βFlt-3c-KIT,对应的IC50值分别为90 nM、20 nM、57 nM、59 nM和68 nM。Sorafenib 可诱导autophagyapoptosis并激活ferroptosis,Sorafenib 具有抗肿瘤活性。
靶点
Raf-1 [1]
(Cell-free assay)
mVEGFR2(Flk1) [1]
(Cell-free assay)
mVEGFR3 [1]
(Cell-free assay)
B-Raf [1]
(Cell-free assay)
B-Raf (V599E) [1]
(Cell-free assay)
点击更多
6 nM 15 nM 20 nM 22 nM 38 nM
体外研究

Sorafenib抑制野生型和V599E突变型B-Raf活性,IC50分别为22 nM 和 38 nM。Sorafenib也能有效抑制mVEGFR2 (Flk-1),mVEGFR3,mPDGFRβ,Flt3,和c-Kit,IC50分别为15 nM,20 nM,57 nM,58 nM,和68 nM。Sorafenib 能够较弱地抑制FGFR-1,IC50 为580 nM。Sorafenib对ERK-1,MEK-1,EGFR,HER-2,IGFR-1,c-Met,PKB,PKA,cdk1/cyclinB,PKCα,PKCγ,和pim-1没有活性。Sorafenib显著抑制NIH 3T3细胞中VEGFR2磷酸化,IC50 为 30 nM,也会抑制HEK-293细胞中Flt-3磷酸化,IC50 为20 nM。Sorafenib有效阻断大多数细胞中MEK 1/2 和 ERK 1/2磷酸化,但不阻断A549 或 H460细胞中该过程,同时不影响PKB通路的抑制。Sorafenib抑制HAoSMC 和 MDA-MB-231细胞的增殖,IC50分别为0.28 μM 和 2.6 μM。[1]除了抑制RAF/MEK/ERK信号通路,Sorafenib tosylate显著抑制eIF4E的磷酸化作用,并以MEK/ERK依赖的方式下调肝癌(HCC)细胞中Mcl-1水平。Sorafenib 抑制PLC/PRF/5 和HepG2细胞的增殖,IC50分别为6.3 μM 和 4.5 μM,并诱导显著的细胞凋亡。[2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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MV4-11 NWj2V5c6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NE\6VIpIUTVyIE2gNE4xOyEQvF2= MmCzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MEi0OlgoRjJzN{C4OFY5RC:jPh?=
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MV4-11 MYTDfZRwfG:6aXPpeJkh[XO|YYm= NXPORoJsT0l3MDC9JFAvODR|IN88US=> Mo\hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MUi3NFkoRjJ|NkG4O|A6RC:jPh?=
MV4-11 M3jXSmZ2dmO2aX;uJIF{e2G7 MX;HTVUxKD1iMD6wOFMh|ryP NWLqcIJtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wPFExOjNpPkK2NFgyODJ|PD;hQi=>
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MOLM13 M4ftRWN6fG:2b4jpZ4l1gSCjc4PhfS=> MmfWS2k2OCB;IECuNFU3KM7:TR?= NHLJPJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xPFcxQSd-MkO2NVg4ODl:L3G+
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Spodoptera frugiperda MofCSpVv[3Srb36gZZN{[Xl? NHTlXZpKSzVyIE2gNE4xQSEQvF2= NHzscm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkmyNlIzQCd-Mk[5NlIzOjh:L3G+
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HepG2 MnrHRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NYXrfGoxTUN3MDC9JFAvOzB{IN88US=> NHvLeJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxNFgyOCd-MkewNVA5OTB:L3G+
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MDA-MB-231 NHrOZnhIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MljxS2k2OCB;IEGuNlYh|ryP M3HnelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
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A375P NEDNTlRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M{jYcmlEPTBiPTCyMlch|ryP MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR4MECzNEc,OjJ2NkCwN|A9N2F-
U937 NIqxeodCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NUnUcJllT0l3MDC9JFIvPzRizszN NX;PfWttRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[zNVgxPjdpPkK2N|E5ODZ5PD;hQi=>
U937 MXnDfZRwfG:6aXPpeJkh[XO|YYm= M3rzS2dKPTBiPTCyMlc1KM7:TR?= NEHIcpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEi3PFE6Oyd-MkS4O|gyQTN:L3G+
MCF7 NGfIfWZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MVLJR|UxKD1iMj63PEDPxE1? NEPqVZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUGwNlE4PSd-MkmxNFIyPzV:L3G+
MDA-MB-231 Moi5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NVP4SmVDUUN3MDC9JFIvQCEQvF2= MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTJyMkSwN{c,Ojl{MEK0NFM9N2F-
HepG2 NE\3R41CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MUfJR|UxKD1iMj64OEDPxE1? NVrhcoNWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiyOFI2PTNpPkK4NlQzPTV|PD;hQi=>
U937 NEDpbXdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MnXQS2k2OCB;IEKuPFUh|ryP MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjBzNEe1OUc,OjJyMUS3OVU9N2F-
A549 MUDDfZRwfG:6aXPpeJkh[XO|YYm= NUWyZWh2UUN3MDC9JFIvQTJizszN NF;2Omw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyO|gxOSd-Mki5Nlc5ODF:L3G+
A549 MmHNR5l1d3SxeHnjbZR6KGG|c3H5 MlWxTWM2OCB;IEKuPVIh|ryP MlnEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5N{ewNFkoRjJ5N{e3NFA6RC:jPh?=
ZR75-30 M2W3PWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MXzJR|UxKD1iMj65OkDPxE1? NX3TV5JLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNFIyPzVpPkK5NVAzOTd3PD;hQi=>
PC3 NInGZldEgXSxdH;4bYNqfHliYYPzZZk> NUjtZotFUUN3MDC9JFMvODNizszN NXGzcJM1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkizOFA6OTNpPkK4N|QxQTF|PD;hQi=>
MDA-MB-231 MkfqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MXzJR|UxKD1iMz6wPEDPxE1? NFrVTG09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5NVk{QCd-Mk[5PVE6Ozh:L3G+
A549 MXTHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Mn3sTWM2OCB;IEOuNUDPxE1? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTFyMkG3OUc,OjlzMEKxO|U9N2F-
K562 M4HZUGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFvKPGxIUTVyIE2gN{4yPiEQvF2= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
MOLT4 NYfwfmRET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlroS2k2OCB;IEOuNVYh|ryP MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
A549/ATCC M3fEUWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWLHTVUxKD1iMz6xOkDPxE1? M4fZfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
RPMI8226 MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NHvjd29VT0liPTCzMlE3KM7:TR?= M4fqNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
SR MnXTS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? Mor4S2k2OCB;IEOuNVYh|ryP MoHOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
HOP62 MoHFS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NGPlb2JVT0liPTCzMlE3KM7:TR?= MmK0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
NCI-H226 M{LUUmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NGHwdmtVT0liPTCzMlE3KM7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
U251 MVnHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NILJWlRVT0liPTCzMlE3KM7:TR?= NIDKXYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
COLO205 MV\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MoD6WGdKKD1iMz6xOkDPxE1? M3yyNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
M14 NV7qPFRHT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MVfUS2khRSB|LkG2JO69VQ>? NITiRos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
HCC2998 M3f5OWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEfMSmNIUTVyIE2gN{4yPiEQvF2= NF25T|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
SNB75 NXXoNJVVT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NFj5RpdIUTVyIE2gN{4yPiEQvF2= M2jwdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
786-0 NFHmeFRIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MX3HTVUxKD1iMz6xOkDPxE1? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
A498 NVfWd3lWT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MkP4WGdKKD1iMz6xOkDPxE1? NWPxelBJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
HCT15 M1PNb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NEPDcVZVT0liPTCzMlE3KM7:TR?= NHOxS|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
OVCAR3 Ml:wS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M{XOd2dKPTBiPTCzMlE3KM7:TR?= MlvCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
OVCAR4 M{DSXWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NFm5Vm9IUTVyIE2gN{4yPiEQvF2= NFvqTYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
Caki1 NVr2VnlZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MljkS2k2OCB;IEOuNVYh|ryP NV7vRVF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
SNB19 NYD5d4liT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NE\hWFdIUTVyIE2gN{4yPiEQvF2= MkT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
OVCAR5 M{\i[Gdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MnTzS2k2OCB;IEOuNVYh|ryP M2L0eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
DU145 NFfybGNIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MlmzS2k2OCB;IEOuNVYh|ryP M2qxcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
OVCAR8 MXLHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NVGwemZHT0l3MDC9JFMvOTZizszN NXnpcZhrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
SN12C M3HaRmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUX2[mw5XEeLIE2gN{4yPiEQvF2= NWj3cmhERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
MDA-MB-231 M3HOSWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MonJWGdKKD1iMz6xOkDPxE1? NHv0Too9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
RXF393 Mnn5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NI[zcHNIUTVyIE2gN{4yPiEQvF2= NWT4VmxtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
BT549 MlO5S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3WxXWdKPTBiPTCzMlE3KM7:TR?= NILIRVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
K562 MlHHR5l1d3SxeHnjbZR6KGG|c3H5 NIfjeXpIUTVyIE2gN{4yPiEQvF2= NF7MZoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
MOLT4 NX;TSphjS3m2b4TvfIlkcXS7IHHzd4F6 NGW3N3FIUTVyIE2gN{4yPiEQvF2= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
SNB19 NGW2N2VEgXSxdH;4bYNqfHliYYPzZZk> MYLHTVUxKD1iMz6xOkDPxE1? NEXvWJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
SR MYLDfZRwfG:6aXPpeJkh[XO|YYm= MWTHTVUxKD1iMz6xOkDPxE1? NFL4XpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
A549 MXfDfZRwfG:6aXPpeJkh[XO|YYm= MXXHTVUxKD1iMz6xOkDPxE1? MknEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
OVCAR3 MmK0R5l1d3SxeHnjbZR6KGG|c3H5 NFLGcWJIUTVyIE2gN{4yPiEQvF2= NX3RNWRNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
OVCAR4 M13MfWN6fG:2b4jpZ4l1gSCjc4PhfS=> NF;afWpIUTVyIE2gN{4yPiEQvF2= MkPtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
SNB75 MX\DfZRwfG:6aXPpeJkh[XO|YYm= NGiwN21IUTVyIE2gN{4yPiEQvF2= NFLXZlQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
OVCAR5 NWrQ[VhmS3m2b4TvfIlkcXS7IHHzd4F6 M2jTUGdKPTBiPTCzMlE3KM7:TR?= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
DU145 NEHnO|lEgXSxdH;4bYNqfHliYYPzZZk> MVfHTVUxKD1iMz6xOkDPxE1? M4jYR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
786-0 MXzDfZRwfG:6aXPpeJkh[XO|YYm= Mn\IS2k2OCB;IEOuNVYh|ryP MoXwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
BT549 NH7WOZlEgXSxdH;4bYNqfHliYYPzZZk> MX\HTVUxKD1iMz6xOkDPxE1? MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
ACHN MULDfZRwfG:6aXPpeJkh[XO|YYm= NFXNUJpIUTVyIE2gN{4yPiEQvF2= NYfIW3RGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
Caki1 Ml7SR5l1d3SxeHnjbZR6KGG|c3H5 NXv0Z403T0l3MDC9JFMvOTZizszN NUfrTJdLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1PVA2ODhpPkK2OVkxPTB6PD;hQi=>
786-0 M3nI[GFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFvVZZRIUTVyIE2gN{4yPiEQvF2= M{D6b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
LOXIMVI MoXxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M2\i[XRIUSB;IEOuNVYh|ryP NWrydnZxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
Caki1 Ml;1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NIfwdnFIUTVyIE2gN{4yPiEQvF2= MoPuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
OVCAR5 M1foXGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NFvuO2tIUTVyIE2gN{4yPiEQvF2= NInQT2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
RXF393 MYjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NV3Ge2wyT0l3MDC9JFMvOTZizszN NUfEXJRTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
SNB75 MnnJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MkOxS2k2OCB;IEOuNVYh|ryP MknXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
OVCAR3 Mo\RRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M4jx[2dKPTBiPTCzMlE3KM7:TR?= NGHLSYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
SNB19 MmfHRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnXyS2k2OCB;IEOuNVYh|ryP NGP3T289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
SK-MEL-5 MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M1jtNnRIUSB;IEOuNVYh|ryP MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
OVCAR4 MlfqRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NGT1[otIUTVyIE2gN{4yPiEQvF2= M3jJR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
SK-MEL-2 Mnz1RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NIL0NZRVT0liPTCzMlE3KM7:TR?= MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
BT549 NGXhepZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MonFS2k2OCB;IEOuNVYh|ryP M4\4XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
MOLT4 MlTJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M3H3SWdKPTBiPTCzMlE3KM7:TR?= NVLiVWptRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
K562 M3f1VWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 Ml71S2k2OCB;IEOuNVYh|ryP NWHjT|dpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HCC2998 M{K5cmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NIHSW2FIUTVyIE2gN{4yPiEQvF2= M4DCN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
HCC2998 NX;ZfohkSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M4rvN3RIUSB;IEOuNVYh|ryP M4\LVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
A549/ATCC NHXlc3hCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NF;vR4xIUTVyIE2gN{4yPiEQvF2= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
DU145 M2i2NmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NVnmZWJlT0l3MDC9JFMvOTZizszN MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SR NXuxbotySW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NYrQc2ZNT0l3MDC9JFMvOTZizszN NVPReHpYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
MCF7 MmLER5l1d3SxeHnjbZR6KGG|c3H5 NFX6VIJKSzVyIE2gN{4yQCEQvF2= NHLEXnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEmyO|gxOSd-Mki5Nlc5ODF:L3G+
PC3 Mn71R5l1d3SxeHnjbZR6KGG|c3H5 MUjJR|UxKD1iMz6xPEDPxE1? Mm\0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh7Mke4NFEoRjJ6OUK3PFAyRC:jPh?=
MCF7 Mo\KR5l1d3SxeHnjbZR6KGG|c3H5 MlLSTWM2OCB;IEOuNVgh|ryP M1nncVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{e3NFA6Lz5{N{e3O|AxQTxxYU6=
A549 NY\4blZmSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NEPXfZNKSzVyIE2gN{4yQSEQvF2= NX:xOWlZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW3O|g6QTVpPkK1O|c5QTl3PD;hQi=>
PC3 NHnzO2JEgXSxdH;4bYNqfHliYYPzZZk> NFzhVItKSzVyIE2gN{4zPCEQvF2= MnPDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjd5N{ewNFkoRjJ5N{e3NFA6RC:jPh?=
HeLa NYTnb|d2TnWwY4Tpc44h[XO|YYm= NWDvZ2NCUUN3MDC9JFMvOyEQvF2= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTJ{NUewOkc,OTV{MkW3NFY9N2F-
HeLa MXvGeY5kfGmxbjDhd5NigQ>? NITYTZFKSzVyIE2gN{4{KM7:TR?= NHvPdGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUKyOVcxPid-MUWyNlU4ODZ:L3G+
LoVo NFXzdmpEgXSxdH;4bYNqfHliYYPzZZk> M{fWb2lEPTBiPTCzMlMh|ryP M2nOflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEW0NFE4Lz5{M{S1OFAyPzxxYU6=
A375 NWrReFZxSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NH3xTpZKSzVyIE2gN{4{PiEQvF2= NHLYT|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OU[wNlY4PCd-Mkm2NFI3PzR:L3G+
HT-29 M1PxTGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MX7JR|UxKD1iMz6zO{DPxE1? NHjmcZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nkm5NVk{QCd-Mk[5PVE6Ozh:L3G+
FLT3 gene-deficient U937 MXXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NHTycXNIUTVyIE2gN{41KM7:TR?= NUSxOnU{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NFg1PjhpPkKxO|A5PDZ6PD;hQi=>
FLT3 negative U937 MmX3R5l1d3SxeHnjbZR6KGG|c3H5 NEfReXhIUTVyIE2gN{41KM7:TR?= M{D4[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkG4O|A6Lz5{M{[xPFcxQTxxYU6=
U937 MUfDfZRwfG:6aXPpeJkh[XO|YYm= MY\HTVUxKD1iMz60JO69VQ>? Mo\mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZyOEGwNlMoRjJ4MEixNFI{RC:jPh?=
A375P M1SwOGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MnTnS2k2OCB;IEOuOEDPxE1? MkfrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2NUmxOFQoRjJ7NEW5NVQ1RC:jPh?=
ACHN MnzKR5l1d3SxeHnjbZR6KGG|c3H5 Ml7nTWM2OCB;IEOuOEDPxE1? NFHYb2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUWxO|kxQCd-Mkm1NVc6ODh:L3G+
ACHN MmXpR5l1d3SxeHnjbZR6KGG|c3H5 NFfEVJRKSzVyIE2gN{41KM7:TR?= MnrEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl{OUe2PFgoRjJ7Mkm3Olg5RC:jPh?=
HepG2 NHP0elFHfW6ldHnvckBie3OjeR?= NV\BNXNQUUN3MDC9JFMvPCEQvF2= NX:xd3U6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[wO|E5PjFpPkK2NFcyQDZzPD;hQi=>
A375P M1XGXGN6fG:2b4jpZ4l1gSCjc4PhfS=> M{nBdGdKPTBiPTCzMlQh|ryP NXizd4t7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS4O|gyQTNpPkK0PFc5OTl|PD;hQi=>
HepG2 NGLNUVFEgXSxdH;4bYNqfHliYYPzZZk> M2fn[2lEPTBiPTCzMlQ1KM7:TR?= NX:0VIY5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5Nlc5ODFpPkK4PVI4QDBzPD;hQi=>
HepG2 NEjydIVEgXSxdH;4bYNqfHliYYPzZZk> NWrPWGJSUUN3MDC9JFMvPDRizszN M1vHOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5N{e3NFA6Lz5{N{e3O|AxQTxxYU6=
ACHN MnnvR5l1d3SxeHnjbZR6KGG|c3H5 M{XtU2lEPTBiPTCzMlUh|ryP MlfhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl5OE[0N|YoRjJ7N{i2OFM3RC:jPh?=
HepG2 M1nyO2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MmfqTWM2OCB;IEOuOUDPxE1? MkXsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV2NkKyOlUoRjJ3NE[yNlY2RC:jPh?=
HT-29 MYnDfZRwfG:6aXPpeJkh[XO|YYm= NVTwZos1UUN3MDC9JFMvPjFizszN MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDh{NkixOUc,OjR6Mk[4NVU9N2F-
HT-29 M3r0ZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NHrvcYdKSzVyIE2gN{43OSEQvF2= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJzNki0PUc,OzB{MU[4OFk9N2F-
HT-29 NYfzV3VuS3m2b4TvfIlkcXS7IHHzd4F6 NEDmSHdKSzVyIE2gN{43OSEQvF2= MofzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjVyOE[yN|goRjJ3MEi2NlM5RC:jPh?=
HT-29 M2TRT2N6fG:2b4jpZ4l1gSCjc4PhfS=> MknrTWM2OCB;IEOuOlEh|ryP MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR2MEi3PUc,OjV2NEC4O|k9N2F-
MCF7 MU\BcpRq[2GwY3XyJIFkfGm4aYT5JIF{e2G7 MU\JR|UxKD1iMz62OEDPxE1? NX7IOmlNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm1OFk5PDFpPkK5OVQ6QDRzPD;hQi=>
Sf9 MoLySpVv[3Srb36gZZN{[Xl? NXuxTFZjUUN3MDC9JFMvQCEQvF2= MnrpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MEi0OlgoRjJzN{C4OFY5RC:jPh?=
Sf9 MUfGeY5kfGmxbjDhd5NigQ>? NF7iVVFKSzVyIE2gN{45KM7:TR?= NGLD[nM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xPFcxQSd-MkO2NVg4ODl:L3G+
Sf9 MVzGeY5kfGmxbjDhd5NigQ>? M{flN2lEPTBiPTCzMlgh|ryP NIraXZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkC4NVAzOyd-Mk[wPFExOjN:L3G+
A2058 NUHKZZZOS3m2b4TvfIlkcXS7IHHzd4F6 M{jFOmlEPTBiPTCzMlgh|ryP MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjdyOEm4O{c,OjJ5MEi5PFc9N2F-
NCI-H460 NVLZd49uS3m2b4TvfIlkcXS7IHHzd4F6 MYjJR|UxKD1iMz65JO69VQ>? M4fqN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEW0NFE4Lz5{M{S1OFAyPzxxYU6=
SK-MEL-30 MkjIR5l1d3SxeHnjbZR6KGG|c3H5 MXPJR|UxKD1iMz65JO69VQ>? M4fJdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NE[xPFI4Lz5{OUS2NVgzPzxxYU6=
HT-29 NX3IS2w{SW62aXPhcoNmeiCjY4Tpeol1gSCjc4PhfS=> Mln6TWM2OCB;IEOuPVch|ryP NH35NW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg1OSd-Mkm1OFk5PDF:L3G+
SF268 NFv4PW9Iem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWHUS2khRSB|Lkm4JO69VQ>? NYHIeGlDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
SNB75 MYPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MlHmWGdKKD1iMz65PEDPxE1? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
OVCAR3 NHK4XlZIem:5dHigbY5pcWKrdHnvckBie3OjeR?= MWfUS2khRSB|Lkm4JO69VQ>? NIjCXoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
SNB19 MVHHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MYPUS2khRSB|Lkm4JO69VQ>? Mk\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
Hs 578T MXrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MkXFWGdKKD1iMz65PEDPxE1? NEXJW2g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
TK10 NWPJZ|JET3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NY\PfW4yT0l3MDC9JFMvQThizszN NHjnXVQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
BT549 Mmi3S5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NHfOcXZVT0liPTCzMlk5KM7:TR?= NEPoZoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
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NCI-H23 MoPhS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MoPwWGdKKD1iNT6wNUDPxE1? M2H1OFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
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786-0 MXfHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH\VPVFVT0liPTC1MlAyKM7:TR?= NVOyZpBDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
MDA-MB-435 NW\QZW8{T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= Mkj6WGdKKD1iNT6wNUDPxE1? M3nFXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
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UACC257 M3PuTGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4nIS3RIUSB;IEWuNFEh|ryP MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
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BT549 NFv0d2lCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M17rPXRIUSB;IEWuNFEh|ryP NHfDOpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
MDA-MB-231 M3rQWGN6fG:2b4jpZ4l1gSCjc4PhfS=> MX7JR|UxKD1iNT6xJO69VQ>? M4W1c|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUW5OFg{Lz5{NkG1PVQ5OzxxYU6=
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A549 M4f0SWN6fG:2b4jpZ4l1gSCjc4PhfS=> M4\3OmlEPTBiPTC1MlIyKM7:TR?= NX\ob2g4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxPFE1OTRpPkKwNVgyPDF2PD;hQi=>
HeLa NIPyN3JEgXSxdH;4bYNqfHliYYPzZZk> MnrZTWM2OCB;IEWuNlMh|ryP NFjWS2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkO0Nlg3Pyd-Mk[zOFI5Pjd:L3G+
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HCT116 M1rHXWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NYTYUGltT0l3MDC9JFUvPCEQvF2= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTJ{NUewOkc,OTV{MkW3NFY9N2F-
A375 MY\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MlTSTWM2OCB;IEWuOEDPxE1? NY[1S2piRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0O|c2ODVpPkG4OFc4PTB3PD;hQi=>
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MCF7 MVPDfZRwfG:6aXPpeJkh[XO|YYm= MYHJR|UxKD1iNT61JO69VQ>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjF3OUS4N{c,OjZzNUm0PFM9N2F-
A375 NFLiPG1CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NUL2epdtUUN3MDC9JFUvPThizszN MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTR4NEi4O{c,OTl2NkS4PFc9N2F-
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A375P NWrOeG85T3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MlnhS2k2OCB;IEWuOVgh|ryP NGP3S|I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0PVY2QCd-MkCxOFk3PTh:L3G+
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A375P M3XTTWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MVjJR|UxKD1iNT62JO69VQ>? NUXRZnpHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm4OVc6PjNpPkG5PFU4QTZ|PD;hQi=>
A375P M1XlZ2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NUTLXWZXUUN3MDC9JFUvPiEQvF2= M172OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUmyOlI5Lz5{MUW5NlYzQDxxYU6=
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A375P M1\ZfmN6fG:2b4jpZ4l1gSCjc4PhfS=> MVjJR|UxKD1iNT62JO69VQ>? MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTh7N{O2Okc,OTl6OUezOlY9N2F-
A375P MkXJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MoHjTWM2OCB;IEWuOkDPxE1? MkTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ4NEe3NlAoRjJ{NkS3O|IxRC:jPh?=
HCT116 NWT2NJlKS3m2b4TvfIlkcXS7IHHzd4F6 M2DoT2lEPTBiPTC1MlY2KM7:TR?= NHvIPZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKxOVgyQCd-MkSyNVU5OTh:L3G+
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HepG2 MmXnR5l1d3SxeHnjbZR6KGG|c3H5 M1fk[2lEPTBiPTC1Mlc1KM7:TR?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MUOxPEc,OjV2NkGzNVg9N2F-
Bel7402 NYewco9tS3m2b4TvfIlkcXS7IHHzd4F6 NGLXdWtKSzVyIE2gOU45KM7:TR?= NYfqOoNCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVQxOTdpPkKzOFU1ODF5PD;hQi=>
HT-29 MV3DfZRwfG:6aXPpeJkh[XO|YYm= MoOzTWM2OCB;IEWuPUDPxE1? MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh4NUK3Okc,Ojh6NkWyO|Y9N2F-
melanoma NUHmdWpNT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= NG\zflNKSzVyIE2gOk4yKM7:TR?= MkjDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ|OUK4NlYoRjF4M{myPFI3RC:jPh?=
K562 MYnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NFnJVIdIUTVyIE2gOk4zKM7:TR?= MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdyOES2PEc,OjF5MEi0Olg9N2F-
HepG2 M1ewUWN6fG:2b4jpZ4l1gSCjc4PhfS=> M2DkTmlEPTBiPTC2MlIh|ryP Mnr1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh6NkWyO|YoRjJ6OE[1Nlc3RC:jPh?=
A549 NVnwNGlPSW62aXPhcoNmeiCjY4Tpeol1gSCjc4PhfS=> Mom5TWM2OCB;IE[uNlEh|ryP NHH1OZA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUW0PVg1OSd-Mkm1OFk5PDF:L3G+
SMMC7721 NGjSdXVCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NH7tSXRKSzVyIE2gOk4zOyEQvF2= NETWRW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkeyNVkzPCd-MkK3NlE6OjR:L3G+
HepG2 NVH6NnlFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M1juOmlEPTBiPTC2MlMh|ryP Mnv0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN7M{KwO|EoRjJ|OUOyNFcyRC:jPh?=
SF295 MX\Hdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? Ml\tWGdKKD1iNj6zNUDPxE1? MmfPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
SK-MEL-2 M3nsPGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MUfUS2khRSB4LkOxJO69VQ>? M{HrcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MEi4NFg3Lz5{OEC4PFA5PjxxYU6=
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UO31 NGLnPXVIem:5dHigbY5pcWKrdHnvckBie3OjeR?= NXTlV4x7XEeLIE2gOk4{OSEQvF2= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
A498 NGm0enJCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MULUS2khRSB4LkOxJO69VQ>? M32ydlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
MALME-3M M3fZW2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MWrUS2khRSB4LkOxJO69VQ>? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
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SK-MEL-2 NGe3XnFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NWP3WW9SVEN3MDC9JFYvOzFizszN NWPTW3l1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
RPMI8226 NXPnOYJ6SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NVvocmk6XEeLIE2gOk4{OSEQvF2= MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
HCC2998 MoC4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M37H[mxEPTBiPTC2MlMyKM7:TR?= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
NCI-H522 NV\zfHQ2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M2jVW3RIUSB;IE[uN|Eh|ryP NVH4fZZ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
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A549 NFHNfWxEgXSxdH;4bYNqfHliYYPzZZk> M{T4emlEPTBiPTC2MlU{KM7:TR?= MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB2M{K2PEc,OjdyNEOyOlg9N2F-
A549 NH[4ZnFEgXSxdH;4bYNqfHliYYPzZZk> NWnYc4Y{UUN3MDC9JFYvPTNizszN Mo\EQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjh|NEC5NVMoRjJ6M{SwPVE{RC:jPh?=
LoVo M4DmSmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MkTkTWM2OCB;IE[uOVYh|ryP MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDNzNUG5Nkc,OjR|MUWxPVI9N2F-
A375 NEG1V2NHfW6ldHnvckBie3OjeR?= NWjvOXB6UUN3MDC9JFYvPTl7IN88US=> MmDJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6MEe1NFcoRjJzOEC3OVA4RC:jPh?=
HCT116 MojiSpVv[3Srb36gZZN{[Xl? M3vNfGdKPTBiPTC2MlYh|ryP MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTJ{NUewOkc,OTV{MkW3NFY9N2F-
HepG2 NGDaRoVEgXSxdH;4bYNqfHliYYPzZZk> MnvHTWM2OCB;IE[uO{DPxE1? M4nqRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MUW5OFg{Lz5{NkG1PVQ5OzxxYU6=
BCPAP MXzBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MX3JR|UxKD1iNj63JO69VQ>? NEjCWHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUCzNlA{OSd-MkmwN|IxOzF:L3G+
A549 NX\jW3VuS3m2b4TvfIlkcXS7IHHzd4F6 NHTCWGFKSzVyIE2gOk44KM7:TR?= MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDh4NUK3Okc,Ojh6NkWyO|Y9N2F-
PC3 NH;veIREgXSxdH;4bYNqfHliYYPzZZk> MoG3TWM2OCB;IE[uPEDPxE1? NGS0PJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{CyNVk3Pyd-MkOwNlE6Pjd:L3G+
SGC7901 MVjDfZRwfG:6aXPpeJkh[XO|YYm= NG[ycnpKSzVyIE2gOk46KM7:TR?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR3NECxO{c,OjN2NUSwNVc9N2F-
OS-RC2 M2O1NGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGj4U4pKSzVyIE2gO{DPxE1? MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTVzN{mwPEc,Ojl3MUe5NFg9N2F-
OS-RC2 Mk\3R5l1d3SxeHnjbZR6KGG|c3H5 MnHuTWM2OCB;IEeg{txO M1rzS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7Mkm3Olg5Lz5{OUK5O|Y5QDxxYU6=
COLO205 MXTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDoeY1idg>? Mn[xTWM2OCB;IEeuNFQh|ryP NEXVXlY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEK0NlU2Oyd-MkiyOFI2PTN:L3G+
rhabdomyosarcoma MUTDfZRwfG:6aXPpeJkh[WO2aY\peJkh[WejaX7zeEBpfW2jbh?= MXvDR|UxKD1iNz6wOUDPxE1? M37QbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5Mki4NVg3Lz5{N{K4PFE5PjxxYU6=
HuH7 NFvOR|FEgXSxdH;4bYNqfHliYYPzZZk> NHW0R2NKSzVyIE2gO{4yKM7:TR?= NWHjXmF2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[xOVk1QDNpPkK2NVU6PDh|PD;hQi=>
MDA-MB-231 NWK1e3FtSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= MVPJR|UxKD1iNz6xPEDPxE1? NELEb3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkeyNVkzPCd-MkK3NlE6OjR:L3G+
FLT3-negative K562 NX71Ro9{S3m2b4TvfIlkcXS7IHHzd4F6 MmPjS2k2OCB;IEeuN{DPxE1? NGPWVGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[xPFcxQSd-MkO2NVg4ODl:L3G+
K562 MXnGeY5kfGmxbjDhd5NigQ>? M1LLVWdKPTBiPTC3MlMh|ryP M2PTTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4MEixNFI{Lz5{NkC4NVAzOzxxYU6=
K562 MYPGeY5kfGmxbjDhd5NigQ>? MWXHTVUxKD1iNz6zJO69VQ>? MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjB6MUCyN{c,OjZyOEGwNlM9N2F-
MCF7 NHPLcFFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NFPpd4pKSzVyIE2gO{4{OyEQvF2= NITHO|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyQSd-MkO2OFQzOTl:L3G+
A549 M1XTSGN6fG:2b4jpZ4l1gSCjc4PhfS=> NVX2fZpiXEeLIE2gO{4{PiEQvF2= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
WI38 MV\DfZRwfG:6aXPpeJkh[XO|YYm= NG[wPHBKSzVyIE2gO{42PCEQvF2= MnTEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6Mk[4NVUoRjJ2OEK2PFE2RC:jPh?=
A375 MVnDfZRwfG:6aXPpeJkh[XO|YYm= Mn;YTWM2OCB;IEeuOVYh|ryP MnnBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR{MUW4NVgoRjJ2MkG1PFE5RC:jPh?=
MDA-MB-231 MWXDfZRwfG:6aXPpeJkh[XO|YYm= MWrJR|UxKD1iNz62NkDPxE1? M2XmXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUixOFE1Lz5{MEG4NVQyPDxxYU6=
786-0 MljlR5l1d3SxeHnjbZR6KGG|c3H5 M{TmcnRIUSB;IEeuOlIh|ryP MoDVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
HS27 M{TnOmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M{HuXGlEPTBiPTC3Mlgh|ryP NHvoTW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUi1O|k3Oyd-MUm4OVc6PjN:L3G+
HS27 MXzDfZRwfG:6aXPpeJkh[XO|YYm= NWHTTXIyUUN3MDC9JFcvQCEQvF2= MoWxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl6OUezOlYoRjF7OEm3N|Y3RC:jPh?=
HCT116 NH7Md3ZEgXSxdH;4bYNqfHliYYPzZZk> M2HvSmlEPTBiPTC3Mlgh|ryP M4j4TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEizOVkzLz5{MkS4N|U6OjxxYU6=
HS27 NHT3Z3VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NV\YTYxxUUN3MDC9JFcvQDVizszN M3f6TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NE[0PFg4Lz5zOUS2OFg5PzxxYU6=
HS27 MorQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MUjHTVUxKD1iNz64OUDPxE1? MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTN3M{W3NUc,OjF|NUO1O|E9N2F-
A549/ATCC NW\qWoFST3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MYPUS2khRSB5Lkm0JO69VQ>? NFThS|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OEC4PFA5Pid-MkiwPFgxQDZ:L3G+
DU145 NG\LVFdIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1G1R3RIUSB;IEeuPVQh|ryP MknYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjhyOEiwPFYoRjJ6MEi4NFg3RC:jPh?=
NCI/ADR-RES M3O4RWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MWPUS2khRSB5Lkm0JO69VQ>? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
MCF7 Mn;kS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M3jZeHRIUSB;IEeuPVQh|ryP MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
PC3 NXnhfXloSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NFrGcXFVT0liPTC3Mlk1KM7:TR?= NVuwVJlrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
UO31 MmraRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NFGxZm1VT0liPTC3Mlk1KM7:TR?= MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
NCI-ADR-RES MWnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MlniWGdKKD1iNz65OEDPxE1? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
LOXIMVI MnTFRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? Mn:2UGM2OCB;IEeuPVQh|ryP M3nkbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
SK-MEL-28 MX3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NUCwPXg{XEeLIE2gO{46PCEQvF2= NF[2O|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
KM12 NF3ROmxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NWH6V4NOXEeLIE2gO{46PCEQvF2= MkfWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
COLO205 MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MWDUS2khRSB5Lkm0JO69VQ>? NVy0[nhxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
HT-29 M4\2NmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MV3US2khRSB5Lkm0JO69VQ>? M2D0[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
NCI-H226 MVjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NELOSZRVT0liPTC3Mlk1KM7:TR?= NFTkT3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
HOP62 MmHJRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NX\YO3Q4XEeLIE2gO{46PCEQvF2= M3z1b|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
T47D NGH1XoxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= M3\yZnRIUSB;IEeuPVQh|ryP NH;1Omw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
MDA-MB-468 NFzIZ2ZCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NFK5SIxVT0liPTC3Mlk1KM7:TR?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
EKVX NFjy[IdCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MUjUS2khRSB5Lkm0JO69VQ>? NHrpcXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
MCF7 MljCRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M{TXN3RIUSB;IEeuPVQh|ryP MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
Hs 578T M4TxOmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NWnHbYhKXEeLIE2gO{46PCEQvF2= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
MGC803 NVHtW4dwSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M37YbWlEPTBiPTC3Mlk6KM7:TR?= M4X5XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NU[wNFQ6Lz5{NkW2NFA1QTxxYU6=
HCT116 MmHkRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MorJTWM2OCB;IEiuNFgh|ryP MmrHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR2NEC0O|koRjJ2NESwOFc6RC:jPh?=
PC3 NIrRNYxEgXSxdH;4bYNqfHliYYPzZZk> MoHuTWM2OCB;IEiuNFgh|ryP MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzB2M{K2PEc,OjdyNEOyOlg9N2F-
HCT116 M3v3XWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 MU\JR|UxKD1iOD6wPEDPxE1? MmXBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVkoRjJ|NkS0NlE6RC:jPh?=
WM266.4 NX;oOHl2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NXzI[IJMT0l3MDC9JFgvOSEQvF2= NYDnbYNLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwNlU6QTZpPkKzNFI2QTl4PD;hQi=>
WM266.4 NV\n[nB2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M4jDT2dKPTBiPTC4MlEzKM7:TR?= MojtQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ3OEO2OlkoRjJ{NUizOlY6RC:jPh?=
Ketr3 NGrkcHRCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MkG3TWM2OCB;IEiuNlch|ryP MoXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOzB{MU[4OFkoRjNyMkG2PFQ6RC:jPh?=
A375 NEf5OolCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= MUnJR|UxKD1iOD6yO{DPxE1? NVmyUIxmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxM{CyNVY5PDlpPkOwNlE3QDR7PD;hQi=>
HepG2 NWD5UYUzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M2jWPGlEPTBiPTC4MlI4KM7:TR?= NHTpfpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEKxOlg1QSd-M{CyNVY5PDl:L3G+
MX1 MUHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NHnSWVNKSzVyIE2gPE4zPyEQvF2= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{ODJzNki0PUc,OzB{MU[4OFk9N2F-
MX1 MnP4R5l1d3SxeHnjbZR6KGG|c3H5 NWjNNpp{UUN3MDC9JFgvOjdizszN MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ5NUGzOUc,OjR4N{WxN|U9N2F-
WM266.4 Mn\wS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? NYDLN5BQT0l3MDC9JFgvOyEQvF2= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjN4MU[4Okc,OjJ|NkG2PFY9N2F-
PLC/PRF/5 M33afmN6fG:2b4jpZ4l1gSCjc4PhfS=> Ml\QTWM2OCB;IEiuN{DPxE1? M2nBPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNUOxNFU{Lz5{MUWzNVA2OzxxYU6=
A375 NFKzdmtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NUTydJU2UUN3MDC9JFgvOzNizszN NGT2XpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUi4OlMzPCd-Mkm4PFY{OjR:L3G+
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PANC1 NHLSbWlEgXSxdH;4bYNqfHliYYPzZZk> M2m2e2lEPTBiPTCxNk4{KM7:TR?= NFPtdm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwNFkzOCd-MkSzNFA6OjB:L3G+
HepG2 MXnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mn;OTWM2OCB;IEGyMlU1KM7:TR?= MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB|MkCzNUc,OjlyM{KwN|E9N2F-
A549 MWnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mm\KTWM2OCB;IEGyMlU1KM7:TR?= Ml7oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVkoRjJ|NkS0NlE6RC:jPh?=
Caki1 M2jkV2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MV3US2khRSBzMj61PUDPxE1? NUfIS25[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkiwPFgxQDZpPkK4NFg5ODh4PD;hQi=>
OVCAR8 M{fxWmdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NH\4eXFVT0liPTCxNk42QSEQvF2= MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
SKOV3 MV3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? MYjUS2khRSBzMj62JO69VQ>? NWfWV3ZrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkewNVc2PDlpPkK3NFE4PTR7PD;hQi=>
ACHN MUnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? Mo\JWGdKKD1iMUKuOkDPxE1? NFHmWG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
OVCAR3 NE\V[GhCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NWjxSpVOXEeLIE2gNVIvPiEQvF2= MorGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
OVCAR4 NHnwNWxCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NYPzVZJ7XEeLIE2gNVIvPiEQvF2= NHzkdow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
HCT15 MonDRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NYXxbWd4XEeLIE2gNVIvPiEQvF2= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
SW620 NYXTUG9VSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M{\LWnRIUSB;IEGyMlYh|ryP MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
A549/ATCC M4DKS2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M{exfHRIUSB;IEGyMlYh|ryP MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
MDA-MB-468 MUPDfZRwfG:6aXPpeJkh[XO|YYm= NUP0fZMzXEeLIE2gNVMvODlizszN M{jK[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
SK-MEL-28 NV\sRWt5S3m2b4TvfIlkcXS7IHHzd4F6 MUDUS2khRSBzMz6zPUDPxE1? M4jTXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ4NUmwOVA5Lz5{NkW5NFUxQDxxYU6=
A549 MoDWRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MXLJR|UxKD1iMUOuOlQh|ryP NYPMfZV6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[1OlAxPDlpPkK2OVYxODR7PD;hQi=>
A375 MlPORY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgbJVu[W5? MlOxTWM2OCB;IEGzMlY1KM7:TR?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDJ2MkW1N{c,Ojh{NEK1OVM9N2F-
A375 NX;xNmFuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M1mxUGlEPTBiPTCxN{43PCEQvF2= NUi3WlRqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW0OlIzPjdpPkK1OFYzOjZ5PD;hQi=>
K562 NYjyRmlGSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NE\me5RKSzVyIE2gNVMvQDVizszN M1zPVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlE6Lz5{M{[0OFIyQTxxYU6=
Hep3B NX3JRZhxS3m2b4TvfIlkcXS7IHHzd4F6 M3XBNWlEPTBiPTCxOE4xQCEQvF2= MkfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MESyNFQoRjJzNUC0NlA1RC:jPh?=
T47D M2XiXmN6fG:2b4jpZ4l1gSCjc4PhfS=> NELr[5JVT0liPTCxOE4{QCEQvF2= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
NCI-H226 Mn3yR5l1d3SxeHnjbZR6KGG|c3H5 NYnnU4RwXEeLIE2gNVQvPDJizszN NGTBR3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NkW5NFUxQCd-Mk[1PVA2ODh:L3G+
MDA-MB-231 MkLzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NXfacHNmUUN3MDC9JFE1NjZ{IN88US=> M4rwR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NESwOFc6Lz5{NES0NFQ4QTxxYU6=
MDA-MB-231 Mki4RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MmrtTWM2OCB;IEG0MlYzKM7:TR?= NIP6Z4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyQSd-MkO2OFQzOTl:L3G+
NCI-H522 MV;DfZRwfG:6aXPpeJkh[XO|YYm= NHjNeIlVT0liPTCxOE45OiEQvF2= MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
SK-MEL-2 NVTHZ|Q2S3m2b4TvfIlkcXS7IHHzd4F6 M4jvb3RIUSB;IEG0Mlg2KM7:TR?= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPjV7MEWwPEc,OjZ3OUC1NFg9N2F-
OS-RC2 M3:zNGN6fG:2b4jpZ4l1gSCjc4PhfS=> NHrzbWFKSzVyIE2gNVUh|ryP MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTd6NkSzOkc,Ojl5OE[0N|Y9N2F-
HepG2 Ml7JRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MnK5TWM2OCB;IEG1JO69VQ>? NFPlbpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUGwN|g4Oyd-MkmxNFM5PzN:L3G+
fibroblast Mk[zRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? M3nj[2lEPTBiPTCxOU4yKM7:TR?= M4\PNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEe3OVA2Lz5zOES3O|UxPTxxYU6=
HT-29 NWXFVIJRS3m2b4TvfIlkcXS7IHHzd4F6 MmnyTWM2OCB;IEG1MlIh|ryP NVnQcY9HRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|UyOzVpPkK0Olc2OTN3PD;hQi=>
U87MG NIiyc|BEgXSxdH;4bYNqfHliYYPzZZk> NWXTV4J[UUN3MDC9JFE2NjV5IN88US=> Mn;IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR6Mk[4NVUoRjJ2OEK2PFE2RC:jPh?=
BT549 NYLBNY5wSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= Mn:2UGM2OCB;IEG1Mlgh|ryP MmjnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
GES-1 NYCzXmdNS3m2b4TvfIlkcXS7IHHzd4F6 NIHPdYRKSzVyIE2gNVUvQCEQvF2= Mm\vQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN6N{G5NFkoRjJ|OEexPVA6RC:jPh?=
SNB19 M3vPU2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 M3rK[nRIUSB;IEG1Mlg2KM7:TR?= M3T5RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
IGROV1 Mn3IRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? Mn\HWGdKKD1iMUWuPUDPxE1? MmDFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjdyMUe1OFkoRjJ5MEG3OVQ6RC:jPh?=
HCT116 M4TWOmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIF{e2G7 NXW3ZWVHVEN3MDC9JFE2NjlizszN M3zQOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
OS-RC2 MVfDfZRwfG:6aXPpeJkh[XO|YYm= NW\lbWFoUUN3MDC9JFE3KM7:TR?= NX;afHFxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0OVQxOTdpPkKzOFU1ODF5PD;hQi=>
A375 NFXTZoREgXSxdH;4bYNqfHliYYPzZZk> Mn\kTWM2OCB;IEG2MlI1KM7:TR?= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MUOxPEc,OjV2NkGzNVg9N2F-
SKOV3 NHX1cFNEgXSxdH;4bYNqfHliYYPzZZk> NGDzfohVT0liPTCxOk4{PiEQvF2= MkjNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
WM1361 MWjBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? NY[0fJJEUUN3MDC9JFE3NjR2IN88US=> NV7KWHI3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm2NFI3PzRpPkK5OlAzPjd2PD;hQi=>
MKN28 NVnVTGNCSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= M1TFN2lEPTBiPTCxO{DPxE1? MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTB|MkCzNUc,OjlyM{KwN|E9N2F-
SMMC7721 M4LIR2N6fG:2b4jpZ4l1gSCjc4PhfS=> Mlf0TWM2OCB;IEG3MlMh|ryP Mn3tQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF3MESyNFQoRjJzNUC0NlA1RC:jPh?=
UACC257 NWnpN2VtS3m2b4TvfIlkcXS7IHHzd4F6 M362dXRIUSB;IEG3MlM{KM7:TR?= MnjSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjZ3OUC1NFgoRjJ4NUmwOVA5RC:jPh?=
EAhy926 MlzwRY51cWGwZ3nv[4VvcWNiYXP0bZZqfHliYYPzZZk> MWDJR|UxKD1iMUiuOVIh|ryP NV\sd4pDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmwN|IxOzFpPkK5NFMzODNzPD;hQi=>
SMMC7721 NUn1[WlvS3m2b4TvfIlkcXS7IHHzd4F6 NEewd5BKSzVyIE2gNVgvPyEQvF2= NFHP[Fc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEOwNFkzOCd-MkSzNFA6OjB:L3G+
Ketr3 MmPLR5l1d3SxeHnjbZR6KGG|c3H5 Mo[xTWM2OCB;IEG4Mlgh|ryP NXvpNo9[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2O|UyOzVpPkK0Olc2OTN3PD;hQi=>
NCI-H522 M1nFdmN6fG:2b4jpZ4l1gSCjc4PhfS=> M2XQfGlEPTBiPTCxPU4zPiEQvF2= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTR4MUOxPEc,OjV2NkGzNVg9N2F-
A549 M3\wbWN6fG:2b4jpZ4l1gSCjc4PhfS=> MXTJR|UxKD1iMUmuOVQh|ryP NFnkfG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{G2NlEzOyd-MkexOlIyOjN:L3G+
SH-SY5Y NEPZO5BEgXSxdH;4bYNqfHliYYPzZZk> NEjsZmNKSzVyIE2gNVkvPTRizszN M3iweFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OEexPVA6Lz5{M{i3NVkxQTxxYU6=
MGC803 MlK0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? MljDTWM2OCB;IEG5MlkzKM7:TR?= NVTyWZAxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkmxNFM5PzNpPkK5NVA{QDd|PD;hQi=>
EKVX M4fseWdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 M4D4VXRIUSB;IEG5Mlk2KM7:TR?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQDB6OEC4Okc,OjhyOEiwPFY9N2F-
OVCAR5 MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDhd5NigQ>? M33P[nRIUSB;IEKwJO69VQ>? NHK4TpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{N{CxO|U1QSd-MkewNVc2PDl:L3G+
UACC62 NHzFT4VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBie3OjeR?= NHLqUZhNSzVyIE2gNlAh|ryP MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPzBzN{W0PUc,OjdyMUe1OFk9N2F-
UACC257 MlLzRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZZN{[Xl? NUTiOXJJVEN3MDC9JFIxKM7:TR?= M1TQXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
MDA-MB-231 NXnUd2FFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[XO|YYm= NVPDfWEzVEN3MDC9JFIxKM7:TR?= M1iye|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ5MEG3OVQ6Lz5{N{CxO|U1QTxxYU6=
U87 M1vOVmN6fG:2b4jpZ4l1gSCjc4PhfS=> M2XqOWlEPTBiPTCyNU4xPyEQvF2= NX\hSoJzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0N|U1PzlpPkKwOFM2PDd7PD;hQi=>
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Assay
Methods Test Index PMID
Western blot LC3-I / LC-3II / ATG5 p-STAT3 / STAT3 / Mcl-1 β-catenin / Survivin / Mcl-1 / PTMA pERK / ERK p-PKM2(y105) / PMK2 / Caspase-9 RET(pY1016) / VEGFR2(pY1214) / MEK1(pT292) / ERK(pY204) Cyclin D1 23392173 26517516 22286758 26959741 22941289 26039995
Growth inhibition assay Cell viability 26039995
Immunofluorescence p65 cytochrome c 22286758 22278289
ELISA TGF-beta / CD206 Caspase-9 / Caspase-3 26158762 30923462
体内研究 Sorafenib(~60 mg/kg)口服给药,对各种人肿瘤异种移植模型,包括MDA-MB-231,Colo-205,HT-29,DLD-1,NCI-H460,和A549表现出广谱的、剂量依赖性抗肿瘤活性,而没有毒性。与抗肿瘤功效相联系,Sorafenib 治疗有效抑制HT-29 和 MDA-MB-231异种移植物中MEK 1/2磷酸化和pERK 1/2水平,但对Colo-205异种移植物没有作用,并且也能显著抑制MDA MB-231,HT-29 和 Colo-205肿瘤异种移植物中肿瘤微血管面积(MVA)和微血管密度(MVD)。[1] 在SCID小鼠体内,Sorafenib治疗对PLC/PRF/5肿瘤异种移植产生剂量依赖性生长抑制,10 mg/kg和30 mg/kg剂量下,TGIs分别为49%和78%,与ERK 和 eIF4E磷酸化抑制,微血管面积减少,和肿瘤细胞凋亡的诱导相一致。[2] Sorafenib通过下调NF-κB介导的Mcl-1 和 cIAP2表达的作用机制使bax-/-细胞对TRAIL剂量依赖性敏感。 Sorafenib (30-60 mg/kg) 与 TRAIL (5 mg/kg)结合对TRAIL抗性HCT116 bax-/-和HT29肿瘤异种移植物表现出显著的功效。[3]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
  • 生化检测:

    重组杆状病毒表达的Raf-1 (残基305–648)和 B-Raf (残基 409–765)以融合蛋白纯化。全长人MEK-1由PCR产生,并以大肠杆菌裂解物中的融合蛋白纯化。将Sorafenib加入到含Raf-1 (80纳克),或B-Raf (80 纳克) 以及MEK-1 (1 微克) 混合物的实验缓冲液[20 mM Tris (pH 8.2),100 mM NaCl,5 mM MgCl2,和0.15% β-巯基乙醇]中,DMSO终浓度为1%。加入25微升10 μM γ[33P]ATP (400 Ci/mol)启动Raf激酶试验(终体积50微升),并在32 °C下培育25分钟。磷酸化的MEK-1通过过滤到磷酸纤维素板上采集,使用1%磷酸洗掉未结合的放射性。微波炉加热干燥后,使用β型板计数器量化过滤器结合放射性。人VEGFR2 (KDR)激酶域被表达,并从Sf9裂解物中纯化。VEGFR2的时间分辨荧光分析法能量转移测试在96孔不透明板中以时间分辨荧光分析法能量转移格式进行。最终反应条件如下:1 到10 μM ATP,25 nM poly GT生物素,2 nM 铕标记的磷酸(p)-酪氨酸抗体(PY20),10 nM APC,1% DMSO 终浓度的1 到 7 nM 细胞质激酶域,50 mM HEPES (pH 7.5),10 mM MgCl2,0.1 mM EDTA,0.015% Brij-35,0.1 毫克/毫升BSA,和0.1% β-巯基乙醇。反应体积为100微升,加入酶启动反应。反应启动~1.5 到 2.0小时后,板以615 和 665 nM在Perkin-Elmer VictorV多标记分析仪上读数。信号按如下比率计算:对每孔(665 nm/615 nM) × 10,000。对IC50的产生,在酶起始反应之前加入Sorafenib。50倍的库存板由Sorafenib在50% DMSO/50%蒸馏水溶液中连续稀释制得。最终Sorafenib在1% DMSO中的浓度范围为10 μM 到 4.56 nM。

细胞实验:[1]
  • Cell lines: MDA-MB-231,和 HAoSMC
  • Concentrations: 溶解于DMSO,终浓度为~10 μM
  • Incubation Time: 72小时
  • Method: 细胞在逐渐增加浓度的Sorafenib下暴露72小时。细胞数通过Cell TiterGlo ATP发光测定试剂盒进行定量。该试验通过测定基于细胞中ATP量的发光信号,测量每孔中的活细胞。
动物实验:[1]
  • Animal Models: 雌性 NCr-nu/nu 小鼠,皮下植入MDA-MB-231,Colo-205,HT-29,H460,或 A549 细胞
  • Dosages: ~60 mg/kg
  • Administration: 口服,每天一次

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
5%DMSO+45%PEG400+50%H2O

0.375mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 464.82
化学式

C21H16ClF3N4O3

CAS号 284461-73-0
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05068752 Recruiting Drug: Vemurafenib|Drug: Sorafenib Pancreas Cancer HonorHealth Research Institute|Bayer|Genentech Inc. October 28 2021 Phase 2
NCT04763408 Recruiting Drug: Lenvatinib|Drug: Sorafenib Carcinoma Hepatocellular Eisai Inc. April 9 2021 --
NCT04000737 Recruiting Drug: YIV-906+Sorafenib|Drug: Placebo+Sorafenib Advanced Hepatocellular Carcinoma Yiviva Inc. January 10 2020 Phase 2
NCT03958669 Recruiting -- Hepatocellular Carcinoma|Sorafenib University Hospital Tuebingen|German Federal Ministry of Education and Research November 1 2019 --
NCT03764293 Active not recruiting Drug: SHR-1210|Drug: Apatinib|Drug: Sorafenib Locally Advanced or Metastatic and Unresectable HCC Jiangsu HengRui Medicine Co. Ltd. June 10 2019 Phase 3

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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