Torin 1

For research use only. Not for use in humans.

目录号:S2827

Torin 1 Chemical Structure

Molecular Weight(MW): 607.62

Torin 1是一种有效的mTORC1/2抑制剂,在无细胞试验中IC50为2 nM/10 nM;作用于mTOR比作用于PI3K选择性高1000倍。

规格 价格 库存 购买数量  
RMB 1419.84 现货
RMB 3019.04 现货
RMB 5492.28 现货
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客户使用Selleck生产的Torin 1发表文献99篇:

产品安全说明书

mTOR抑制剂选择性比较

生物活性

产品描述 Torin 1是一种有效的mTORC1/2抑制剂,在无细胞试验中IC50为2 nM/10 nM;作用于mTOR比作用于PI3K选择性高1000倍。
靶点
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
体外研究

Torin1在2和10 nM浓度下分别抑制mTORC1 和mTORC2底物的磷酸化。此外,Torin1对mTOR比对PI3K (EC50 = 1800 nM)的选择性高1000倍,比对450种其它蛋白激酶的结合选择性高100倍。[1] [2] Torin1通过耐rapamycin机制引起细胞周期阻滞,并且其不依赖于mTORC2。Torin1比rapamycin更完全地干扰mTORC1依赖表现型。Cap依赖性翻译需要mTORC1耐Rapamycin的功能。[1]在近期的一项研究中,据报道Torin1通过活化人内分泌细胞系BON 中MEK/ERK/c-Jun通路,能够增加神经降压素分泌和基因表达。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 NGXVZoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PyfWlEPTB;MD6wNFA{ODZizszN MXvTRW5ITVJ?
OCUB-M Mo[4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LieWlEPTB;MD6wNFE6PCEQvF2= M3HEO3NCVkeHUh?=
DOHH-2 MlHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTBwMECyNlUh|ryP MX\TRW5ITVJ?
NOS-1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIW3dVBKSzVyPUCuNFAzPDJizszN M4DDZ3NCVkeHUh?=
L-363 MlX2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGiyeFJKSzVyPUCuNFAzQDRizszN NWLreIw6W0GQR1XS
RL95-2 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LheWlEPTB;MD6wNFMzKM7:TR?= MlTCV2FPT0WU
BL-70 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3OxWWlEPTB;MD6wNFM{PyEQvF2= M{HQPHNCVkeHUh?=
TGBC1TKB MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jyTWlEPTB;MD6wNFM4QSEQvF2= NHfWRXNUSU6JRWK=
697 NV7PfoU{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTBwMEC2N|Qh|ryP MnTGV2FPT0WU
RPMI-8226 Ml\xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3[1TGlEPTB;MD6wNFY2OSEQvF2= NInQbnVUSU6JRWK=
BL-41 NFi4PXhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnHzTWM2OD1yLkCwOlk3KM7:TR?= MYfTRW5ITVJ?
MRK-nu-1 NVG5eYVlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzxdWUxUUN3ME2wMlAxPzB5IN88US=> MWnTRW5ITVJ?
EW-1 MnXJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTBwMEC3OFkh|ryP NF\aW3ZUSU6JRWK=
EVSA-T M2P6emdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoTQTWM2OD1yLkCwPFkzKM7:TR?= M2HYRXNCVkeHUh?=
HUTU-80 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{f4V2lEPTB;MD6wNFkzQSEQvF2= MWDTRW5ITVJ?
VA-ES-BJ NFjKdYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3joOGlEPTB;MD6wNFk5PCEQvF2= MVjTRW5ITVJ?
GCIY Ml;BS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2Tt[mlEPTB;MD6wNVAxPyEQvF2= MXPTRW5ITVJ?
ES6 NGLCdGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUjZdoxsUUN3ME2wMlAyODh|IN88US=> NI\5VFRUSU6JRWK=
NALM-6 M4iw[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnMTWM2OD1yLkCxNVAzKM7:TR?= NVXy[oJOW0GQR1XS
LB996-RCC NIHkXVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTBwMEGxN|ch|ryP MVTTRW5ITVJ?
EW-16 M{jafGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTBwMEGxPVIh|ryP MoLOV2FPT0WU
KALS-1 Mki2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTBwMEGxPVgh|ryP MlezV2FPT0WU
OPM-2 NFPCNGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXlTIFKSzVyPUCuNFEzQSEQvF2= MlP4V2FPT0WU
KU812 Moq2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHsTWM2OD1yLkCxNlk3KM7:TR?= Moe0V2FPT0WU
A4-Fuk M37pdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHVT|MyUUN3ME2wMlAyOzF7IN88US=> NIWyfWNUSU6JRWK=
KGN MmTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTBwMEGzOVkh|ryP NVThV2Q3W0GQR1XS
KS-1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzSTWM2OD1yLkCxN|kzKM7:TR?= NF\BcYJUSU6JRWK=
no-11 Mnu3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEm1e2pKSzVyPUCuNFE{QTVizszN Ml;5V2FPT0WU
PSN1 Ml23S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7Pe5hKSzVyPUCuNFE1ODlizszN NGHxW4JUSU6JRWK=
SK-LMS-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3TiVmlEPTB;MD6wNVQyOyEQvF2= M1TZVHNCVkeHUh?=
LS-411N NH7SOoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XsV2lEPTB;MD6wNVQ{QSEQvF2= MUDTRW5ITVJ?
HT-144 NF;TZZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTBwMEG0OVEh|ryP MV\TRW5ITVJ?
ACN NF7CdIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTBwMEG1JO69VQ>? M3XhfnNCVkeHUh?=
RKO M2fSW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTBwMEG1N|Mh|ryP M4HWV3NCVkeHUh?=
CMK MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXaTWM2OD1yLkCxOVQ{KM7:TR?= M13oOHNCVkeHUh?=
GB-1 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW[xUm9XUUN3ME2wMlAyPTV|IN88US=> M3PoNHNCVkeHUh?=
KE-37 MlTFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLtTWM2OD1yLkCxOVc{KM7:TR?= NGj4PIZUSU6JRWK=
no-10 MkG1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7z[G5LUUN3ME2wMlAyPTh2IN88US=> NVT6fFJqW0GQR1XS
MOLT-16 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1HXdGlEPTB;MD6wNVYzOiEQvF2= M{nQZ3NCVkeHUh?=
HEL NF7CPYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTBwMEG2OEDPxE1? NVTHNWh5W0GQR1XS
NCI-H1355 NVLEUpdGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXLJR|UxRTBwMEG2OFYh|ryP MkXFV2FPT0WU
IST-MEL1 M4jvNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUHlO3UyUUN3ME2wMlAyPzd2IN88US=> Ml\tV2FPT0WU
MHH-PREB-1 NX75[lR7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XmO2lEPTB;MD6wNVgyQCEQvF2= NH;YeotUSU6JRWK=
ES8 MlfNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjQXWVWUUN3ME2wMlAyQDR2IN88US=> M4HM[nNCVkeHUh?=
LC-2-ad MlK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTBwMEKxN|ch|ryP MlfoV2FPT0WU
LAMA-84 M4LoTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTBwMEKxOlch|ryP M3TNdnNCVkeHUh?=
BV-173 Ml;HS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M32xWmlEPTB;MD6wNlIxOyEQvF2= MmfhV2FPT0WU
SW872 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUTDPY0xUUN3ME2wMlAzOjR5IN88US=> NHG3TWhUSU6JRWK=
SK-UT-1 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWW5XnU1UUN3ME2wMlAzOjZzIN88US=> NX70eIszW0GQR1XS
OS-RC-2 MmfRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlntTWM2OD1yLkCyN|I{KM7:TR?= MY\TRW5ITVJ?
NCI-H747 M1TucWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVzjcG9IUUN3ME2wMlAzOzNizszN Mm[yV2FPT0WU
MZ2-MEL NVW4fpd2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIDWXFJKSzVyPUCuNFI{OzdizszN NYjsOIgxW0GQR1XS
TE-11 M{DGcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTBwMEKzO|Ih|ryP MY\TRW5ITVJ?
KURAMOCHI NWnDN3hjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rETGlEPTB;MD6wNlQyPCEQvF2= MlzKV2FPT0WU
HD-MY-Z MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWqzTottUUN3ME2wMlAzPDJ4IN88US=> MWfTRW5ITVJ?
GI-1 M4iwS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTBwMEK0Olkh|ryP NUK0UFRJW0GQR1XS
LB1047-RCC M1jQRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1;3eGlEPTB;MD6wNlQ5QCEQvF2= MlLlV2FPT0WU
TE-12 NV;HWFZ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTCeXVKSzVyPUCuNFI2PSEQvF2= MVvTRW5ITVJ?
MZ7-mel M4f5XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmrnTWM2OD1yLkCyOlA6KM7:TR?= NYjrdm1MW0GQR1XS
MMAC-SF MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XtRmlEPTB;MD6wNlY{OyEQvF2= MYTTRW5ITVJ?
A101D NGXRWIFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzoTWM2OD1yLkCyOlUh|ryP MWHTRW5ITVJ?
GR-ST MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIe1O3hKSzVyPUCuNFI3PTVizszN MlnOV2FPT0WU
MC116 MnXxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUnJR|UxRTBwMEK2PEDPxE1? NUDjSoZGW0GQR1XS
Ramos-2G6-4C10 NEHKVYJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfSSHVKSzVyPUCuNFI4PSEQvF2= NEf2fZlUSU6JRWK=
ATN-1 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DQPGlEPTB;MD6wNlc5QSEQvF2= M1;kOXNCVkeHUh?=
MFH-ino NEPzZ|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUDJR|UxRTBwMEK4NVIh|ryP MmL4V2FPT0WU
DEL NUe4UGN[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7aTWM2OD1yLkCyPFE2KM7:TR?= NE\rS|FUSU6JRWK=
OCI-AML2 MlrOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTBwMEK4O|Eh|ryP NW\1WIRHW0GQR1XS
LXF-289 MoG3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4jIPGlEPTB;MD6wNlg4OiEQvF2= M2n0eHNCVkeHUh?=
IA-LM M1f0[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnnJTWM2OD1yLkCyPVE4KM7:TR?= MVHTRW5ITVJ?
EoL-1-cell NVTjRXROT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mom5TWM2OD1yLkCyPVk3KM7:TR?= M3PGVXNCVkeHUh?=
OVCAR-4 NV24[INZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTBwMEOwPVQh|ryP M33ZZXNCVkeHUh?=
ES1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVL1[GpIUUN3ME2wMlA{ODl5IN88US=> NE\qR4lUSU6JRWK=
CESS M4fxR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfpOI5KSzVyPUCuNFMxQTlizszN MVrTRW5ITVJ?
GAK NHnOXVhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfUN3BYUUN3ME2wMlA{OTh4IN88US=> MYrTRW5ITVJ?
NTERA-S-cl-D1 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmTlTWM2OD1yLkCzNlI3KM7:TR?= MWLTRW5ITVJ?
TE-10 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzzWmpKSzVyPUCuNFM{OTFizszN MYHTRW5ITVJ?
CCRF-CEM NHztRZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVn6RoE6UUN3ME2wMlA{OzdzIN88US=> MUHTRW5ITVJ?
NMC-G1 MlXnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHf3RmZKSzVyPUCuNFM1PDhizszN M3vscnNCVkeHUh?=
HOP-62 M2LaTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIPhU2ZKSzVyPUCuNFM2PTZizszN NUnrTINxW0GQR1XS
SH-4 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnm4TWM2OD1yLkCzOVgzKM7:TR?= M1LhU3NCVkeHUh?=
NCI-H1648 NH61c4RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH\SVmxKSzVyPUCuNFM3OjJizszN M2frOnNCVkeHUh?=
EB-3 MkDzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnnbWpWUUN3ME2wMlA{PjR|IN88US=> MmHhV2FPT0WU
BB65-RCC MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTBwMEO2PVEh|ryP Mo[yV2FPT0WU
BE-13 M3LnTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLsUlRpUUN3ME2wMlA{PzV7IN88US=> MVPTRW5ITVJ?
CP67-MEL NY\ZNoZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\MS|dKSzVyPUCuNFM4PTlizszN M4rTb3NCVkeHUh?=
SW954 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYjlVFN3UUN3ME2wMlA{PzlizszN NWjGSHFQW0GQR1XS
TE-5 NFrZclhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MW\JR|UxRTBwMEO4OVQh|ryP M{C4O3NCVkeHUh?=
HCE-4 NHv2N3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2D5NWlEPTB;MD6wN|g2PSEQvF2= NV;TNZhFW0GQR1XS
LC-1F MkewS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LiXmlEPTB;MD6wN|k5QSEQvF2= MmnxV2FPT0WU
TK10 MmfnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoPuTWM2OD1yLkC0NFE6KM7:TR?= MW\TRW5ITVJ?
SJSA-1 MorXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Moe4TWM2OD1yLkC0NVg5KM7:TR?= M4LEbnNCVkeHUh?=
Daudi MkDwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHMTWM2OD1yLkC0NlI2KM7:TR?= MkL6V2FPT0WU
SW982 MnuyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYPJR|UxRTBwMESyOFYh|ryP NVj0bpJXW0GQR1XS
SF126 M3jXeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIjJZ4lKSzVyPUCuNFQ{PjFizszN NIHWVIFUSU6JRWK=
TE-15 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTBwMES0N|Uh|ryP NXPNdVZYW0GQR1XS
COLO-684 NYPRNm1uT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PHemlEPTB;MD6wOFU3OSEQvF2= MnjYV2FPT0WU
8-MG-BA MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDGT2lKSzVyPUCuNFQ3OjlizszN MmjYV2FPT0WU
NB13 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkDZTWM2OD1yLkC0O|c3KM7:TR?= NIi5ZndUSU6JRWK=
HCC1599 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\KSo9jUUN3ME2wMlA1QDd7IN88US=> NVnpem5vW0GQR1XS
SK-PN-DW M3zyUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M364dmlEPTB;MD6wOFk1QSEQvF2= NYTyUoZzW0GQR1XS
ALL-PO NUH0PVlVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjPO|VKSzVyPUCuNFQ6PTZizszN M2HkeHNCVkeHUh?=
NB6 NUK1bIpYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTBwMEWyO|Eh|ryP MYPTRW5ITVJ?
IST-SL2 NYXjfY1[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M13xemlEPTB;MD6wOVM3OiEQvF2= MUDTRW5ITVJ?
BB30-HNC MmPES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTBwMEW3N{DPxE1? NXruS|luW0GQR1XS
SW962 NGrtSmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmjITWM2OD1yLkC1O|Y{KM7:TR?= MXrTRW5ITVJ?
TGBC24TKB M3jtNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfFTWM2OD1yLkC1O|k6KM7:TR?= MlL6V2FPT0WU
KINGS-1 NVPXOVZ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWHJR|UxRTBwMEW4NFMh|ryP NELiTpFUSU6JRWK=
HC-1 Mmm2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{nmSWlEPTB;MD6wOVgzPyEQvF2= NEPHT3BUSU6JRWK=
RS4-11 M1jYd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWPJR|UxRTBwMEW5N|gh|ryP M3H3dnNCVkeHUh?=
LS-123 M1XaR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVLNfoRuUUN3ME2wMlA2QTR{IN88US=> NYS2SWxiW0GQR1XS
A3-KAW Mm\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTNeGlyUUN3ME2wMlA3OjJ3IN88US=> NGHV[lBUSU6JRWK=
SNB75 NIPVWZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVjrRXNKUUN3ME2wMlA3OzRzIN88US=> MUHTRW5ITVJ?
SR M{\BXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvXZlltUUN3ME2wMlA3Ozd2IN88US=> MYfTRW5ITVJ?
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NOMO-1 MlW5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDn[FJTUUN3ME2wMlM6QTdzIN88US=> NVX5N4ZGW0GQR1XS
MS-1 NYfDZWM4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIPPdVRKSzVyPUCuOFAyPzdizszN NH;5XWFUSU6JRWK=
EKVX MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfyPHNMUUN3ME2wMlQ1PDZzIN88US=> M1[4TXNCVkeHUh?=
SK-MEL-2 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2\PTWlEPTB;MD60OlQh|ryP NUfuZmM2W0GQR1XS
HCC1187 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXKwdJhEUUN3ME2wMlQ3PzZ6IN88US=> NUXjZoRkW0GQR1XS
HCC2218 M332NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zoXmlEPTB;MD60O|IzPyEQvF2= MXPTRW5ITVJ?
NCI-H187 M4W2d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\GRYpKSzVyPUCuOFc5PCEQvF2= NEHSUWpUSU6JRWK=
DB MlW1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NInae29KSzVyPUCuOVAxPTVizszN Ml;RV2FPT0WU
NCI-H69 NEHSSpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV3JR|UxRTBwNUCyOFch|ryP M33tSXNCVkeHUh?=
NCI-H1694 NH;F[WxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTBwNUC2PVch|ryP NIjsbZlUSU6JRWK=
CA46 MlnVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLKTWM2OD1yLkWwPVE4KM7:TR?= MkX6V2FPT0WU
EHEB MoK2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfxUGtmUUN3ME2wMlUyOTl|IN88US=> NXjh[HN{W0GQR1XS
NCCIT NXL2e5Q1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTBwNUSzNlEh|ryP M{XEXXNCVkeHUh?=
NCI-H720 NGm2[nJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnqyTWM2OD1yLkW0PVYzKM7:TR?= MVPTRW5ITVJ?
LU-134-A MmTNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1PhemlEPTB;MD61OVI6PiEQvF2= NGfq[WFUSU6JRWK=
MC-CAR M4T0O2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4GxUWlEPTB;MD61OVQ{OSEQvF2= NVXEd|FZW0GQR1XS
NEC8 MnOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3RV4tKSzVyPUCuOVYyPzJizszN Mn;SV2FPT0WU
NCI-H524 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nqdGlEPTB;MD61PFc1QCEQvF2= NVftSVVoW0GQR1XS
NCI-H1963 NX;QPFdyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1TYPWlEPTB;MD62NlU5KM7:TR?= NVywcmJqW0GQR1XS
SU-DHL-1 NX7RSXJPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mon3TWM2OD1yLk[0OVI3KM7:TR?= NYfzV2lzW0GQR1XS
NCI-H1522 M{HRPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnvTWM2OD1yLk[0PFg{KM7:TR?= M1HqUHNCVkeHUh?=
NCI-H1155 M2Sx[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUDOZlZ4UUN3ME2wMlY2OzVizszN M3XNfXNCVkeHUh?=
D-263MG NIq3[YJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkjSTWM2OD1yLk[2NVAyKM7:TR?= MXvTRW5ITVJ?
TE-6 NUjJfZhXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTBwNkixNFQh|ryP MUTTRW5ITVJ?
NCI-H1417 MnvmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWjpbVJ5UUN3ME2wMlY6PzFzIN88US=> MWLTRW5ITVJ?
NCI-H510A MlTIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHn4WWhKSzVyPUCuO|czOzZizszN MWjTRW5ITVJ?
KARPAS-45 NYrzSVdCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYHJR|UxRTBwN{iyN|Uh|ryP MnnmV2FPT0WU
IST-SL1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEexZ4NKSzVyPUCuPFU5OjVizszN MVPTRW5ITVJ?
COLO-824 NFfQW3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\FSGlEPTB;MD64O|g{PSEQvF2= M{PSTXNCVkeHUh?=
NCI-H526 NIjkWpdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTBwOUC5OVgh|ryP NXvlbXV1W0GQR1XS
NCI-H719 Ml;lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4XCV2lEPTB;MD65OlY2PyEQvF2= NYPLOFVOW0GQR1XS
SF539 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1rWZmlEPTB;MD65PFM6PiEQvF2= NITpSmlUSU6JRWK=
NKM-1 MofSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml6zTWM2OD1yLkm4OlY{KM7:TR?= M4TkdnNCVkeHUh?=
A253 MmXaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYfKfZY6UUN3ME2wMlk5QDh3IN88US=> MorWV2FPT0WU
TE-441-T M4TJcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvXSlVKSzVyPUGuNFQ{PSEQvF2= MmDmV2FPT0WU
COR-L88 NIHhS4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTFwMUGzOVQh|ryP MnO0V2FPT0WU
CPC-N Mnu0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1z0fmlEPTB;MT6xOVg5QCEQvF2= M2KxSXNCVkeHUh?=
NCI-H2107 M1ztWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mke0TWM2OD1zLkG3NlYyKM7:TR?= NInpUYpUSU6JRWK=
ECC4 NV7vOoliT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTFwMUi2PVMh|ryP Ml\5V2FPT0WU
NCI-H2141 NHfO[5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml7WTWM2OD1zLkG5Nlk6KM7:TR?= M3;UZnNCVkeHUh?=
NCI-H1770 NY[xNYluT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{jPOGlEPTB;MT6zNlkzQCEQvF2= NH3OWJZUSU6JRWK=
NCI-SNU-1 NVn2boV{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MljPTWM2OD1zLkOzNFQh|ryP NYG3bolMW0GQR1XS
LU-165 NFrtSoVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mkn2TWM2OD1zLkS1OFY4KM7:TR?= MV;TRW5ITVJ?
Raji MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1K3e2lEPTB;MT60O|I2QSEQvF2= MYfTRW5ITVJ?
EW-24 MkC0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEiwboZKSzVyPUGuOVQ3OTlizszN M{XJeHNCVkeHUh?=
KP-N-RT-BM-1 NWXHb|hVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3KwRmlEPTB;MT61OlU2OSEQvF2= MlvyV2FPT0WU
SK-MEL-1 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfh[|dKSzVyPUGuOlQ5PzdizszN NWfFOpZ3W0GQR1XS
SCLC-21H MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{PETmlEPTB;MT62OlY2OSEQvF2= MoTLV2FPT0WU
SIMA M3v5c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLhS|U{UUN3ME2xMlY5OjN|IN88US=> MUDTRW5ITVJ?
NCI-H1304 MknKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTFwN{C3OlUh|ryP NGnoS45USU6JRWK=
REH M2O2dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MknWTWM2OD1zLkexNFc5KM7:TR?= MV\TRW5ITVJ?
KP-N-YS M1;vRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFv1TlFKSzVyPUGuPVM2PTFizszN M2XBPXNCVkeHUh?=
DMS-153 MkiwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU\JR|UxRTFwOUe1PUDPxE1? NWj3bm04W0GQR1XS
NB10 M3HkPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDKWVcyUUN3ME2yMlIxQDd6IN88US=> NHnLWo1USU6JRWK=
NCI-H345 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTJwNkG5NFUh|ryP M3:4XHNCVkeHUh?=
SK-N-FI M{nNeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTJwN{WzPFEh|ryP MVvTRW5ITVJ?
NCI-H1092 NILJVJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTJwOUWzPVQh|ryP NHvrPXFUSU6JRWK=
KM-H2 MoXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWG0ZoRmUUN3ME2zMlY2Ozl4IN88US=> MoP0V2FPT0WU
KG-1 M3rW[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnThTWM2OD1|LkexOlg5KM7:TR?= MnXRV2FPT0WU
D-283MED NW[5NZJnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU\JR|UxRTNwN{W4O{DPxE1? MkK5V2FPT0WU
LU-65 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDifFFKSzVyPUSuNVE1QDVizszN NVy3UHg1W0GQR1XS
MSTO-211H M4\EdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWniTnpFUUN3ME20MlE5QDl6IN88US=> NWXZO29mW0GQR1XS
NCI-H2081 NHPXRXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PYO2lEPTB;ND6yPVE4PCEQvF2= MWTTRW5ITVJ?
D-247MG NYG3UnBvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnI[2FJUUN3ME20MlQ{OTlzIN88US=> NYfaXGZPW0GQR1XS
P31-FUJ MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrDfYFCUUN3ME20MlQ2ODF7IN88US=> MlPGV2FPT0WU
DMS-79 NHvSTpZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXfJR|UxRTRwOUOxPFMh|ryP NEXrVXBUSU6JRWK=
KASUMI-1 MmXVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPZTWM2OD12Lkm3OVA6KM7:TR?= NHjyfFJUSU6JRWK=
JVM-3 NWjJV4ZMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2roTmlEPTB;NT6yOVAyPCEQvF2= NHH2coZUSU6JRWK=
LAN-6 M2rjfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHOSVVKSzVyPUWuOVE4OzZizszN Mk\DV2FPT0WU
DJM-1 MlfSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1P0[WlEPTB;NT61OlAzQSEQvF2= MVLTRW5ITVJ?
JVM-2 NHXOPYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3;iTmlEPTB;NT62O|A6OiEQvF2= MXXTRW5ITVJ?
U-87-MG M1nRc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTzfG12UUN3ME21Mlg6QTZ2IN88US=> MlHkV2FPT0WU
SHP-77 Mmf6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mny4TWM2OD13Lkm0PVI5KM7:TR?= NUfYVXVoW0GQR1XS
RPMI-8866 Mki3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV[3PZhkUUN3ME22MlI4ODF|IN88US=> MWDTRW5ITVJ?
ES7 NUXs[mJVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjSPHhuUUN3ME22MlM6QTJ2IN88US=> NUnvbnNNW0GQR1XS
WSU-NHL NWG2RnF2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTZwNUG2NFkh|ryP MYnTRW5ITVJ?
CAS-1 NFHRdVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHaSINKSzVyPUeuNFk4PDlizszN M{C4SnNCVkeHUh?=
MHH-NB-11 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRThwMkOxOVIh|ryP MkPKV2FPT0WU
ES5 NFvmNmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPXTWM2OD17LkWyNlg3KM7:TR?= NYLNUGtJW0GQR1XS
HDLM-2 MlHTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFHsNG5KSzVyPUmuPVU6ODJizszN MlTvV2FPT0WU
NCI-H2171 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mlr0TWM2OD1zMD61PVI4KM7:TR?= MoXyV2FPT0WU
LNCaP-Clone-FGC MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3XFd2lEPTB;MUGuN|cyQCEQvF2= MnrIV2FPT0WU
NCI-H1395 M1zsOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHzmSlZKSzVyPUGyMlI3QTlizszN NIPkT|hUSU6JRWK=
NCI-H1436 NEHXTlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{[wWGlEPTB;MUOuN|E6PSEQvF2= M3npN3NCVkeHUh?=
RCC10RGB Ml:4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4K2RWlEPTB;MUOuN|IzOSEQvF2= MYTTRW5ITVJ?
SW684 M3y1Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDzSVN{UUN3ME2xN{46Pzl5IN88US=> NEPyN5dUSU6JRWK=
NCI-H1650 M4rqXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{Tj[mlEPTB;MUWuNFQ1PyEQvF2= NFPBUGVUSU6JRWK=
EW-13 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUTEUox5UUN3ME2xOU4zODJzIN88US=> MU\TRW5ITVJ?
EC-GI-10 M37wR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4nid2lEPTB;MUWuPFY1PCEQvF2= NYP2WWlEW0GQR1XS
CHP-126 NUHm[Y5JT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\DfWpKSzVyPUG3MlE6OThizszN MXHTRW5ITVJ?
NCI-H226 NYHMfJRbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2PwWWlEPTB;MUiuNFQxOyEQvF2= M3nYbHNCVkeHUh?=
NCI-H1838 M1:zS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUPJR|UxRTJyLkG4OFgh|ryP NXvRTFZkW0GQR1XS
EW-11 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzlTWM2OD1{ND6yO|E5KM7:TR?= NEPGdnhUSU6JRWK=
EB2 NYDnfXp[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4TISWlEPTB;M{GuOVMyQCEQvF2= NWXOfFhnW0GQR1XS
D-502MG MoHtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHmyXohKSzVyPUSzMlkzOjlizszN NWizZY9xW0GQR1XS
U-698-M MoDsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnxTWM2OD12OD64OlcyKM7:TR?= MWfTRW5ITVJ?
ES3 NYXlPZVvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\nfWlEPTB;NEmuN|g6OyEQvF2= M1LEenNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
体内研究 Torin1在20 mg/kg剂量下,在U87MG异种移植模型中是有效的,并且在肿瘤和外周组织中对mTOR下游效应蛋白表现出良好的药效学抑制作用。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

mTORC1和 mTORC2体外激酶试验:

为产生可溶性mTORC1,HEK-293T细胞系,稳定表达N端FLAG标记的Raptor,使用水泡性口炎病毒G-假型病毒MSCV逆转录酶病毒产生。对于mTORC2,产生相似的HeLa细胞,能够稳定表达N端FLAG-标记的Protor-1。两个复合物通过在50 mm HEPES,pH 7.4,10 mm 焦磷酸钠,10 mm β-甘油磷酸钠,100 mm NaCl,2 mm EDTA,0.3% CHAPS中裂解细胞纯化。细胞在4 °C下裂解30分钟,不溶解部分通过以13,000 rpm微量离心10分钟移除。上清液与FLAG-M2单克隆抗体-琼脂糖培育1小时,然后用裂解缓冲液洗涤3次,用包含终浓度0.5 M NaCl的裂解缓冲液洗涤1次。纯化的mTORC1用100 μg/mL 3×FLAG多肽在50 mm HEPES,pH 7.4,100 mm NaCl中洗脱。将洗出液等分收集,储存在-80 °C。纯化底物S6K1 和 Akt1。激酶试验在30 °C,由激酶缓冲液(25 mm HEPES,pH 7.4,50 mm KCl,10 mm MgCl2,500 μm ATP)和150 ng 灭活的S6K1或Akt1底物组成的20 μL终体积混合物中进行。反应通过加入80 μL样品缓冲液停止,并沸煮5分钟。随后,样品通过SDS-PAGE和免疫印迹法分析。
细胞实验:[1]
- 合并
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4天
  • Method: 细胞活性通过CellTiter-Glo发光法细胞活性测定评估。在第0天,细胞以500细胞每孔接种到96孔板,并生长过夜。第1天,细胞用适当的化合物处理,随后在第3-5天分析。对于分析,板在室温下培育60分钟,将50 μL CellTiter-Glo试剂加入每孔中,板在定轨摇床上混合12分钟。发光在标准平板光度计上定量。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: U87MG 异种移植物模型
  • Formulation: Torin1粉末首先以25 mg/mL在100% N-甲基-2-吡咯烷酮中溶解,随后以1:4 与无菌 50% PEG400稀释到5 mg/mL的终浓度
  • Dosages: 20 mg/kg
  • Administration: 腹腔注射给药,每天一次
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble
体内 从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
20% NMP+50% PEG 400
5mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 607.62
化学式

C35H28F3N5O2

CAS号 1222998-36-8
储存条件 粉状
溶于溶剂
别名 N/A

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How to re-constitute the inhibitor for i.p. administration?

  • 回答:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID