Torin 1

For research use only. Not for use in humans.

目录号:S2827

Torin 1 Chemical Structure

CAS No. 1222998-36-8

Torin 1是一种有效的mTORC1/2抑制剂,在无细胞试验中IC50为2 nM/10 nM;作用于mTOR比作用于PI3K选择性高1000倍。

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客户使用Selleck生产的Torin 1发表文献121篇:

产品安全说明书

mTOR抑制剂选择性比较

生物活性

产品描述 Torin 1是一种有效的mTORC1/2抑制剂,在无细胞试验中IC50为2 nM/10 nM;作用于mTOR比作用于PI3K选择性高1000倍。
靶点
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
体外研究

Torin1在2和10 nM浓度下分别抑制mTORC1 和mTORC2底物的磷酸化。此外,Torin1对mTOR比对PI3K (EC50 = 1800 nM)的选择性高1000倍,比对450种其它蛋白激酶的结合选择性高100倍。[1] [2] Torin1通过耐rapamycin机制引起细胞周期阻滞,并且其不依赖于mTORC2。Torin1比rapamycin更完全地干扰mTORC1依赖表现型。Cap依赖性翻译需要mTORC1耐Rapamycin的功能。[1]在近期的一项研究中,据报道Torin1通过活化人内分泌细胞系BON 中MEK/ERK/c-Jun通路,能够增加神经降压素分泌和基因表达。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTBwMECwN|A3KM7:TR?= MoLDV2FPT0WU
OCUB-M MnHnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTBwMECxPVQh|ryP M2nWZXNCVkeHUh?=
DOHH-2 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXUZlNJUUN3ME2wMlAxOjJ3IN88US=> MnTJV2FPT0WU
NOS-1 M{eyR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRTBwMECyOFIh|ryP MXLTRW5ITVJ?
L-363 NVnmUZp{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXPx[WZ7UUN3ME2wMlAxOjh2IN88US=> MULTRW5ITVJ?
RL95-2 M4Puemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVnDRpZxUUN3ME2wMlAxOzJizszN MYTTRW5ITVJ?
BL-70 M1XENGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmCzTWM2OD1yLkCwN|M4KM7:TR?= MnzOV2FPT0WU
TGBC1TKB Ml74S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jBVmlEPTB;MD6wNFM4QSEQvF2= MYTTRW5ITVJ?
697 M3vnb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXrJR|UxRTBwMEC2N|Qh|ryP M4DxV3NCVkeHUh?=
RPMI-8226 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXS4eYRWUUN3ME2wMlAxPjVzIN88US=> M1HPUXNCVkeHUh?=
BL-41 NGnnT3BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY\3fYo{UUN3ME2wMlAxPjl4IN88US=> NULNVHN2W0GQR1XS
MRK-nu-1 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfuW4M2UUN3ME2wMlAxPzB5IN88US=> MVnTRW5ITVJ?
EW-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLsTWM2OD1yLkCwO|Q6KM7:TR?= NH\aOnlUSU6JRWK=
EVSA-T Mn62S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGH3XYlKSzVyPUCuNFA5QTJizszN MY\TRW5ITVJ?
HUTU-80 Ml[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzHb2RKSzVyPUCuNFA6OjlizszN MX;TRW5ITVJ?
VA-ES-BJ MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTBwMEC5PFQh|ryP MojyV2FPT0WU
GCIY NH7kSoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXUUmg5UUN3ME2wMlAyODB5IN88US=> MkHTV2FPT0WU
ES6 M3rxWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmTITWM2OD1yLkCxNFg{KM7:TR?= M2fRN3NCVkeHUh?=
NALM-6 NELxTHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoXXTWM2OD1yLkCxNVAzKM7:TR?= MmrmV2FPT0WU
LB996-RCC NYXPbnAzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlLDTWM2OD1yLkCxNVM4KM7:TR?= NUTnT5hKW0GQR1XS
EW-16 NUTjR4h7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUjXcHVGUUN3ME2wMlAyOTl{IN88US=> NWnJVpFnW0GQR1XS
KALS-1 M1riemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWLmTWE5UUN3ME2wMlAyOTl6IN88US=> MX3TRW5ITVJ?
OPM-2 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnFTWM2OD1yLkCxNlkh|ryP Mn64V2FPT0WU
KU812 M332S2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVXJR|UxRTBwMEGyPVYh|ryP MlS0V2FPT0WU
A4-Fuk NWDCSZNwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVv2UY9HUUN3ME2wMlAyOzF7IN88US=> NUHBV3VDW0GQR1XS
KGN M13QNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXv6TVFiUUN3ME2wMlAyOzV7IN88US=> NXjnTGZnW0GQR1XS
KS-1 MlvGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\VS2lEPTB;MD6wNVM6OiEQvF2= NV3ZSXVtW0GQR1XS
no-11 NVe5bnlHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYXJR|UxRTBwMEGzPVUh|ryP NFnUfY1USU6JRWK=
PSN1 NX3KfHhST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M33LPGlEPTB;MD6wNVQxQSEQvF2= NImxeZdUSU6JRWK=
SK-LMS-1 NVrv[VRPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEnvUYtKSzVyPUCuNFE1OTNizszN NYrQcWs{W0GQR1XS
LS-411N NXThUpJFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjQOo5KSzVyPUCuNFE1OzlizszN M1vWbHNCVkeHUh?=
HT-144 NXnL[HU4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTBwMEG0OVEh|ryP MknMV2FPT0WU
ACN M13pcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLZTWM2OD1yLkCxOUDPxE1? NXi3eZN4W0GQR1XS
RKO M2PpZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2fiZ2lEPTB;MD6wNVU{OyEQvF2= Mm[5V2FPT0WU
CMK Mn:xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPxUYdKSzVyPUCuNFE2PDNizszN NIXae45USU6JRWK=
GB-1 M2jiS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3HJXmlEPTB;MD6wNVU2OyEQvF2= MUPTRW5ITVJ?
KE-37 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTBwMEG1O|Mh|ryP M3f3U3NCVkeHUh?=
no-10 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnPlTWM2OD1yLkCxOVg1KM7:TR?= MUPTRW5ITVJ?
MOLT-16 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NE\nfJRKSzVyPUCuNFE3OjJizszN NFvRWplUSU6JRWK=
HEL NUfrZnNNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\KTWM2OD1yLkCxOlQh|ryP NFe5fIJUSU6JRWK=
NCI-H1355 MnX6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF7wOYRKSzVyPUCuNFE3PDZizszN MknBV2FPT0WU
IST-MEL1 M13lXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NW\me2pOUUN3ME2wMlAyPzd2IN88US=> NYTlToVXW0GQR1XS
MHH-PREB-1 M1LKPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVTJR|UxRTBwMEG4NVgh|ryP NW\XfWdSW0GQR1XS
ES8 M37WXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF\wfGFKSzVyPUCuNFE5PDRizszN NYT1RZV4W0GQR1XS
LC-2-ad M1HneWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LsT2lEPTB;MD6wNlE{PyEQvF2= MnToV2FPT0WU
LAMA-84 Mm\YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIW0b|JKSzVyPUCuNFIyPjdizszN MmjRV2FPT0WU
BV-173 MnHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoH1TWM2OD1yLkCyNlA{KM7:TR?= MmqwV2FPT0WU
SW872 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzMc3ZKSzVyPUCuNFIzPDdizszN M2XhUXNCVkeHUh?=
SK-UT-1 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M373O2lEPTB;MD6wNlI3OSEQvF2= MV7TRW5ITVJ?
OS-RC-2 NIrwPYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXnJR|UxRTBwMEKzNlMh|ryP MVjTRW5ITVJ?
NCI-H747 M3fS[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTBwMEKzN{DPxE1? NV;RcIh4W0GQR1XS
MZ2-MEL NE\RcWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4Lz[WlEPTB;MD6wNlM{PyEQvF2= NGW3NlVUSU6JRWK=
TE-11 NX21TpJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITPNlhKSzVyPUCuNFI{PzJizszN MWHTRW5ITVJ?
KURAMOCHI MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWm2[2lQUUN3ME2wMlAzPDF2IN88US=> M1LQVXNCVkeHUh?=
HD-MY-Z M33DR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnv2TWM2OD1yLkCyOFI3KM7:TR?= NYfsfZhTW0GQR1XS
GI-1 NI[3TG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2Hxe2lEPTB;MD6wNlQ3QSEQvF2= M3ntSHNCVkeHUh?=
LB1047-RCC Ml;hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M17JOGlEPTB;MD6wNlQ5QCEQvF2= NW[0XotlW0GQR1XS
TE-12 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\zXXR7UUN3ME2wMlAzPTVizszN M1HOfHNCVkeHUh?=
MZ7-mel M{fDeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rHXWlEPTB;MD6wNlYxQSEQvF2= M1TkZ3NCVkeHUh?=
MMAC-SF MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;zTWM2OD1yLkCyOlM{KM7:TR?= MV7TRW5ITVJ?
A101D MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXraV2dsUUN3ME2wMlAzPjVizszN MXLTRW5ITVJ?
GR-ST Mmj0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXSSHdKSzVyPUCuNFI3PTVizszN M3jCe3NCVkeHUh?=
MC116 M4\FTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFX0RnZKSzVyPUCuNFI3QCEQvF2= NIT0TWZUSU6JRWK=
Ramos-2G6-4C10 NEW2TpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TWPWlEPTB;MD6wNlc2KM7:TR?= MlHFV2FPT0WU
ATN-1 NUDGVHc4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFPrbY9KSzVyPUCuNFI4QDlizszN NVm0R|dLW0GQR1XS
MFH-ino M1LLTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHrWpdKSzVyPUCuNFI5OTJizszN NGXicFhUSU6JRWK=
DEL M3LwTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETGUZFKSzVyPUCuNFI5OTVizszN NGXxSW1USU6JRWK=
OCI-AML2 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTBwMEK4O|Eh|ryP NYHUeW4zW0GQR1XS
LXF-289 M{joTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTBwMEK4O|Ih|ryP MWDTRW5ITVJ?
IA-LM MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUWxNoJIUUN3ME2wMlAzQTF5IN88US=> NWHkOogzW0GQR1XS
EoL-1-cell NYjiW|dpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4rncWlEPTB;MD6wNlk6PiEQvF2= MXLTRW5ITVJ?
OVCAR-4 NVTwdpRPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVLJR|UxRTBwMEOwPVQh|ryP NVn6NplZW0GQR1XS
ES1 M4fkXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjtXmxKSzVyPUCuNFMxQTdizszN NW\UXXpWW0GQR1XS
CESS MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYHjWmRTUUN3ME2wMlA{ODl7IN88US=> NGH0OldUSU6JRWK=
GAK MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGGwZm9KSzVyPUCuNFMyQDZizszN M3XsOHNCVkeHUh?=
NTERA-S-cl-D1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MorVTWM2OD1yLkCzNlI3KM7:TR?= MVHTRW5ITVJ?
TE-10 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmjsTWM2OD1yLkCzN|EyKM7:TR?= NWTGRYp2W0GQR1XS
CCRF-CEM NV3hdXZHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3Lzc2lEPTB;MD6wN|M4OSEQvF2= NX3UcnJ4W0GQR1XS
NMC-G1 MnPBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3u1T2lEPTB;MD6wN|Q1QCEQvF2= MWrTRW5ITVJ?
HOP-62 MkjSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofsTWM2OD1yLkCzOVU3KM7:TR?= NV\FZ2hxW0GQR1XS
SH-4 M4LZXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4TBTmlEPTB;MD6wN|U5OiEQvF2= NUSzZoVuW0GQR1XS
NCI-H1648 MoPLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXTJR|UxRTBwMEO2NlIh|ryP NFrXSlJUSU6JRWK=
EB-3 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnlTWM2OD1yLkCzOlQ{KM7:TR?= MUDTRW5ITVJ?
BB65-RCC NUfSTIlST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XiV2lEPTB;MD6wN|Y6OSEQvF2= NG\ISYpUSU6JRWK=
BE-13 NV7FN3JUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfDVYVuUUN3ME2wMlA{PzV7IN88US=> MYjTRW5ITVJ?
CP67-MEL M{PNN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYGxZVRTUUN3ME2wMlA{PzV7IN88US=> MVzTRW5ITVJ?
SW954 M{PUPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXSxZ4RCUUN3ME2wMlA{PzlizszN NF3kT3pUSU6JRWK=
TE-5 M1S1VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LPXGlEPTB;MD6wN|g2PCEQvF2= NGX0ZlNUSU6JRWK=
HCE-4 Mn3wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLzTWM2OD1yLkCzPFU2KM7:TR?= NWL4SpI4W0GQR1XS
LC-1F Ml35S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjGTWM2OD1yLkCzPVg6KM7:TR?= MkjnV2FPT0WU
TK10 NFThRWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHn6T4xKSzVyPUCuNFQxOTlizszN MkfBV2FPT0WU
SJSA-1 M{D5fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFTOPJNKSzVyPUCuNFQyQDhizszN MlTFV2FPT0WU
Daudi MnfHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml\oTWM2OD1yLkC0NlI2KM7:TR?= M{fMN3NCVkeHUh?=
SW982 NF7Qc4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHPrW3ZKSzVyPUCuNFQzPDZizszN M3rqdXNCVkeHUh?=
SF126 Mn;1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\nTWM2OD1yLkC0N|YyKM7:TR?= MoXzV2FPT0WU
TE-15 M4jVWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTNfXc5UUN3ME2wMlA1PDN3IN88US=> MoTlV2FPT0WU
COLO-684 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYLJR|UxRTBwMES1OlEh|ryP MnfzV2FPT0WU
8-MG-BA NUXYSYNsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnLoTWM2OD1yLkC0OlI6KM7:TR?= NGHHNnJUSU6JRWK=
NB13 MlPUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYLYPYZCUUN3ME2wMlA1Pzd4IN88US=> MX\TRW5ITVJ?
HCC1599 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfBTWM2OD1yLkC0PFc6KM7:TR?= NWD6S4loW0GQR1XS
SK-PN-DW NGTIO2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MU\JR|UxRTBwMES5OFkh|ryP Moj1V2FPT0WU
ALL-PO MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYTJR|UxRTBwMES5OVYh|ryP M{DJUXNCVkeHUh?=
NB6 M2\QV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlrCTWM2OD1yLkC1NlcyKM7:TR?= MXzTRW5ITVJ?
IST-SL2 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoG3TWM2OD1yLkC1N|YzKM7:TR?= MnK0V2FPT0WU
BB30-HNC MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoPVTWM2OD1yLkC1O|Mh|ryP M2CzcXNCVkeHUh?=
SW962 NHjWZVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nVSWlEPTB;MD6wOVc3OyEQvF2= M1PsS3NCVkeHUh?=
TGBC24TKB NUn3d5lTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PlVWlEPTB;MD6wOVc6QSEQvF2= NVflSGhbW0GQR1XS
KINGS-1 M4nPXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTBwMEW4NFMh|ryP NYPORoM{W0GQR1XS
HC-1 NEPSWJFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1vJO2lEPTB;MD6wOVgzPyEQvF2= MUHTRW5ITVJ?
RS4-11 NH\0WHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTBwMEW5N|gh|ryP MYDTRW5ITVJ?
LS-123 MkPvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLlW41EUUN3ME2wMlA2QTR{IN88US=> MmnUV2FPT0WU
A3-KAW MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTBwME[yNlUh|ryP NHv0fXRUSU6JRWK=
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EKVX MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGHiT3VKSzVyPUCuOFQ1PjFizszN NUKyb3VyW0GQR1XS
SK-MEL-2 NEmxb3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnizTWM2OD1yLkS2OEDPxE1? NV20ZVB{W0GQR1XS
HCC1187 MnrJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjZTWM2OD1yLkS2O|Y5KM7:TR?= MYDTRW5ITVJ?
HCC2218 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3rEV2lEPTB;MD60O|IzPyEQvF2= MnLrV2FPT0WU
NCI-H187 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUfJR|UxRTBwNEe4OEDPxE1? NXj6Nop5W0GQR1XS
DB NVPp[I45T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHriXldKSzVyPUCuOVAxPTVizszN NEnxWGdUSU6JRWK=
NCI-H69 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVGwZpBoUUN3ME2wMlUxOjR5IN88US=> MmXFV2FPT0WU
NCI-H1694 M1HLSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3ITWM2OD1yLkWwOlk4KM7:TR?= MnvpV2FPT0WU
CA46 NITCepJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTHTWM2OD1yLkWwPVE4KM7:TR?= MYDTRW5ITVJ?
EHEB MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4fabWlEPTB;MD61NVE6OyEQvF2= M4n3Z3NCVkeHUh?=
NCCIT NWPXV2tOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4raWWlEPTB;MD61OFMzOSEQvF2= M2G0XnNCVkeHUh?=
NCI-H720 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEnlcoRKSzVyPUCuOVQ6PjJizszN MUnTRW5ITVJ?
LU-134-A MljzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTBwNUWyPVYh|ryP NVrENmtKW0GQR1XS
MC-CAR MlK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTBwNUW0N|Eh|ryP NGjvcWtUSU6JRWK=
NEC8 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlzmTWM2OD1yLkW2NVczKM7:TR?= M3HxUHNCVkeHUh?=
NCI-H524 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HhTmlEPTB;MD61PFc1QCEQvF2= NXHHOYd6W0GQR1XS
NCI-H1963 MorxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX30PHl2UUN3ME2wMlYzPThizszN M{PabHNCVkeHUh?=
SU-DHL-1 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3X5UWlEPTB;MD62OFUzPiEQvF2= MWPTRW5ITVJ?
NCI-H1522 NVq4VYkyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWixWJFLUUN3ME2wMlY1QDh|IN88US=> NUDGdVl7W0GQR1XS
NCI-H1155 NGjzcFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFL6UYpKSzVyPUCuOlU{PSEQvF2= NImxcphUSU6JRWK=
D-263MG Ml;0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGf5Z2hKSzVyPUCuOlYyODFizszN M4rNOHNCVkeHUh?=
TE-6 NU[2V2dMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTwcVc3UUN3ME2wMlY5OTB2IN88US=> NGPoXllUSU6JRWK=
NCI-H1417 M4nmRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTBwNkm3NVEh|ryP NHHNR5pUSU6JRWK=
NCI-H510A MlqyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mme4TWM2OD1yLke3NlM3KM7:TR?= NXLtT5piW0GQR1XS
KARPAS-45 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUL1OGdCUUN3ME2wMlc5OjN3IN88US=> MnzWV2FPT0WU
IST-SL1 NXy0SlVlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjhTWM2OD1yLki1PFI2KM7:TR?= M{DXWnNCVkeHUh?=
COLO-824 NWjrWod5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml64TWM2OD1yLki3PFM2KM7:TR?= MXzTRW5ITVJ?
NCI-H526 NF7i[VRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml:3TWM2OD1yLkmwPVU5KM7:TR?= M3XNUXNCVkeHUh?=
NCI-H719 NHuxO4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEnsPFRKSzVyPUCuPVY3PTdizszN NETxXWZUSU6JRWK=
SF539 NWP0dmQ4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnYbmV4UUN3ME2wMlk5Ozl4IN88US=> NYXSbHVlW0GQR1XS
NKM-1 M4LDc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHjENVBKSzVyPUCuPVg3PjNizszN NXruSW5LW0GQR1XS
A253 NWTmbYNUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojETWM2OD1yLkm4PFg2KM7:TR?= NI\2WmlUSU6JRWK=
TE-441-T M2TI[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1LZRWlEPTB;MT6wOFM2KM7:TR?= Ml;4V2FPT0WU
COR-L88 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGjvXYRKSzVyPUGuNVE{PTRizszN NV:3eHl7W0GQR1XS
CPC-N MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDlfHlKSzVyPUGuNVU5QDhizszN M3zHe3NCVkeHUh?=
NCI-H2107 MkHNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1y0eWlEPTB;MT6xO|I3OSEQvF2= MkCyV2FPT0WU
ECC4 M3LxT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoTBTWM2OD1zLkG4Olk{KM7:TR?= M1Tud3NCVkeHUh?=
NCI-H2141 NWXBZZhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTFwMUmyPVkh|ryP NV2zeoJwW0GQR1XS
NCI-H1770 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWLJR|UxRTFwM{K5Nlgh|ryP M1Lt[3NCVkeHUh?=
NCI-SNU-1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkfmTWM2OD1zLkOzNFQh|ryP M3z4[HNCVkeHUh?=
LU-165 M{fR[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUjMXJhSUUN3ME2xMlQ2PDZ5IN88US=> MoXPV2FPT0WU
Raji NHfnd29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWS2[Y96UUN3ME2xMlQ4OjV7IN88US=> MUnTRW5ITVJ?
EW-24 MlfPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTFwNUS2NVkh|ryP MkDkV2FPT0WU
KP-N-RT-BM-1 NULFZWdoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTwTWM2OD1zLkW2OVUyKM7:TR?= Mlf0V2FPT0WU
SK-MEL-1 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYjJR|UxRTFwNkS4O|ch|ryP MVzTRW5ITVJ?
SCLC-21H Mo\5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUOyeZZ4UUN3ME2xMlY3PjVzIN88US=> NG\xeINUSU6JRWK=
SIMA MlvLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH7ncldKSzVyPUGuOlgzOzNizszN MYnTRW5ITVJ?
NCI-H1304 M2\XNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmnFTWM2OD1zLkewO|Y2KM7:TR?= NHf6OIdUSU6JRWK=
REH MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NESxZ4JKSzVyPUGuO|ExPzhizszN M1PiS3NCVkeHUh?=
KP-N-YS MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{m0OWlEPTB;MT65N|U2OSEQvF2= NIDkZldUSU6JRWK=
DMS-153 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4ixWGlEPTB;MT65O|U6KM7:TR?= MmrHV2FPT0WU
NB10 M4LSXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVXkO3hKUUN3ME2yMlIxQDd6IN88US=> M4DrOHNCVkeHUh?=
NCI-H345 Ml\OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M374eGlEPTB;Mj62NVkxPSEQvF2= M173[HNCVkeHUh?=
SK-N-FI MnnHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoKwTWM2OD1{Lke1N|gyKM7:TR?= MYDTRW5ITVJ?
NCI-H1092 NVrzbHh3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlnPTWM2OD1{Lkm1N|k1KM7:TR?= NG\reVZUSU6JRWK=
KM-H2 NEnMfFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV22N2JQUUN3ME2zMlY2Ozl4IN88US=> MYnTRW5ITVJ?
KG-1 M{XpOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3RTWM2OD1|LkexOlg5KM7:TR?= MWLTRW5ITVJ?
D-283MED M4i1Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4DvXWlEPTB;Mz63OVg4KM7:TR?= NGPWenpUSU6JRWK=
LU-65 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVrFPYRUUUN3ME20MlEyPDh3IN88US=> NX:wZ5hzW0GQR1XS
MSTO-211H MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFf3NJhKSzVyPUSuNVg5QThizszN MV;TRW5ITVJ?
NCI-H2081 M{LqWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;vSIFyUUN3ME20MlI6OTd2IN88US=> MojIV2FPT0WU
D-247MG MmDxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDLWoJZUUN3ME20MlQ{OTlzIN88US=> MUDTRW5ITVJ?
P31-FUJ MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnjaTWM2OD12LkS1NFE6KM7:TR?= MnvnV2FPT0WU
DMS-79 NIr3UVRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLofmVRUUN3ME20Mlk{OTh|IN88US=> NF3rdJdUSU6JRWK=
KASUMI-1 NG\yZYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXHJR|UxRTRwOUe1NFkh|ryP NH76VnRUSU6JRWK=
JVM-3 NYjscoxqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlvWTWM2OD13LkK1NFE1KM7:TR?= Mk\sV2FPT0WU
LAN-6 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{fOd2lEPTB;NT61NVc{PiEQvF2= NWLqXGdWW0GQR1XS
DJM-1 NYH0ZZhKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoH6TWM2OD13LkW2NFI6KM7:TR?= M4LsdHNCVkeHUh?=
JVM-2 MlfJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LlVmlEPTB;NT62O|A6OiEQvF2= NVL6Uok5W0GQR1XS
U-87-MG NUO2NpVWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVzJR|UxRTVwOEm5OlQh|ryP NVTqT5lvW0GQR1XS
SHP-77 NYXyWoY3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmnpTWM2OD13Lkm0PVI5KM7:TR?= NF;ZdJZUSU6JRWK=
RPMI-8866 NEW5SYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{frOWlEPTB;Nj6yO|AyOyEQvF2= M{fW[nNCVkeHUh?=
ES7 MoTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkTpTWM2OD14LkO5PVI1KM7:TR?= MX\TRW5ITVJ?
WSU-NHL Mo[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTZwNUG2NFkh|ryP MlHIV2FPT0WU
CAS-1 M3TNdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX\JR|UxRTdwMEm3OFkh|ryP MUnTRW5ITVJ?
MHH-NB-11 NVfOS5V3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrnO3FbUUN3ME24MlI{OTV{IN88US=> M1j4cHNCVkeHUh?=
ES5 NHrJb2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUf0RZN6UUN3ME25MlUzOjh4IN88US=> MoPRV2FPT0WU
HDLM-2 Ml74S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HQXGlEPTB;OT65OVkxOiEQvF2= MlLiV2FPT0WU
NCI-H2171 M2\OVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTFyLkW5Nlch|ryP NIS5dGxUSU6JRWK=
LNCaP-Clone-FGC NXn4O4pyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVzSNYFFUUN3ME2xNU4{PzF6IN88US=> MkC0V2FPT0WU
NCI-H1395 NIXJeI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTF{LkK2PVkh|ryP MXPTRW5ITVJ?
NCI-H1436 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4jpNmlEPTB;MUOuN|E6PSEQvF2= NV\3N4h2W0GQR1XS
RCC10RGB MnewS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVP5b4lvUUN3ME2xN{4{OjJzIN88US=> M3vIfXNCVkeHUh?=
SW684 NIHWZYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTF|Lkm3PVch|ryP MnnLV2FPT0WU
NCI-H1650 MkPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYHBO2g{UUN3ME2xOU4xPDR5IN88US=> MX3TRW5ITVJ?
EW-13 M4Die2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTF3LkKwNlEh|ryP NEDt[4dUSU6JRWK=
EC-GI-10 NFTu[JpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorFTWM2OD1zNT64OlQ1KM7:TR?= NYTpTmNSW0GQR1XS
CHP-126 MkXIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rtUGlEPTB;MUeuNVkyQCEQvF2= Mmr0V2FPT0WU
NCI-H226 NH;1dZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXrJR|UxRTF6LkC0NFMh|ryP MlLMV2FPT0WU
NCI-H1838 NGHzWHdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PmUWlEPTB;MkCuNVg1QCEQvF2= NFKxc|lUSU6JRWK=
EW-11 M{fxS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX;JR|UxRTJ2LkK3NVgh|ryP NHXtb|NUSU6JRWK=
EB2 NHL3TmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTNzLkWzNVgh|ryP MX3TRW5ITVJ?
D-502MG MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTR|LkmyNlkh|ryP NHL0ZldUSU6JRWK=
U-698-M NYnPeox6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NG\jZo5KSzVyPUS4Mlg3PzFizszN MWHTRW5ITVJ?
ES3 NUGyO5d1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7mTWM2OD12OT6zPFk{KM7:TR?= MUHTRW5ITVJ?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
体内研究 Torin1在20 mg/kg剂量下,在U87MG异种移植模型中是有效的,并且在肿瘤和外周组织中对mTOR下游效应蛋白表现出良好的药效学抑制作用。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

mTORC1和 mTORC2体外激酶试验:

为产生可溶性mTORC1,HEK-293T细胞系,稳定表达N端FLAG标记的Raptor,使用水泡性口炎病毒G-假型病毒MSCV逆转录酶病毒产生。对于mTORC2,产生相似的HeLa细胞,能够稳定表达N端FLAG-标记的Protor-1。两个复合物通过在50 mm HEPES,pH 7.4,10 mm 焦磷酸钠,10 mm β-甘油磷酸钠,100 mm NaCl,2 mm EDTA,0.3% CHAPS中裂解细胞纯化。细胞在4 °C下裂解30分钟,不溶解部分通过以13,000 rpm微量离心10分钟移除。上清液与FLAG-M2单克隆抗体-琼脂糖培育1小时,然后用裂解缓冲液洗涤3次,用包含终浓度0.5 M NaCl的裂解缓冲液洗涤1次。纯化的mTORC1用100 μg/mL 3×FLAG多肽在50 mm HEPES,pH 7.4,100 mm NaCl中洗脱。将洗出液等分收集,储存在-80 °C。纯化底物S6K1 和 Akt1。激酶试验在30 °C,由激酶缓冲液(25 mm HEPES,pH 7.4,50 mm KCl,10 mm MgCl2,500 μm ATP)和150 ng 灭活的S6K1或Akt1底物组成的20 μL终体积混合物中进行。反应通过加入80 μL样品缓冲液停止,并沸煮5分钟。随后,样品通过SDS-PAGE和免疫印迹法分析。
细胞实验:[1]
- 合并
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4天
  • Method: 细胞活性通过CellTiter-Glo发光法细胞活性测定评估。在第0天,细胞以500细胞每孔接种到96孔板,并生长过夜。第1天,细胞用适当的化合物处理,随后在第3-5天分析。对于分析,板在室温下培育60分钟,将50 μL CellTiter-Glo试剂加入每孔中,板在定轨摇床上混合12分钟。发光在标准平板光度计上定量。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: U87MG 异种移植物模型
  • Dosages: 20 mg/kg
  • Administration: 腹腔注射给药,每天一次
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 2 mg/mL (3.29 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 607.62
化学式

C35H28F3N5O2

CAS号 1222998-36-8
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、MSDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How to re-constitute the inhibitor for i.p. administration?

  • 回答:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID