Torin 1

For research use only. Not for use in humans.

目录号:S2827

Torin 1 Chemical Structure

CAS No. 1222998-36-8

Torin 1是一种有效的mTORC1/2抑制剂,在无细胞试验中IC50为2 nM/10 nM;作用于mTOR比作用于PI3K选择性高1000倍。

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RMB 1419.84 现货
RMB 5492.28 现货
RMB 15315.3 现货
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客户使用Selleck生产的Torin 1发表文献170篇:

产品安全说明书

mTOR抑制剂选择性比较

生物活性

产品描述 Torin 1是一种有效的mTORC1/2抑制剂,在无细胞试验中IC50为2 nM/10 nM;作用于mTOR比作用于PI3K选择性高1000倍。
靶点
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
体外研究

Torin1在2和10 nM浓度下分别抑制mTORC1 和mTORC2底物的磷酸化。此外,Torin1对mTOR比对PI3K (EC50 = 1800 nM)的选择性高1000倍,比对450种其它蛋白激酶的结合选择性高100倍。[1] [2] Torin1通过耐rapamycin机制引起细胞周期阻滞,并且其不依赖于mTORC2。Torin1比rapamycin更完全地干扰mTORC1依赖表现型。Cap依赖性翻译需要mTORC1耐Rapamycin的功能。[1]在近期的一项研究中,据报道Torin1通过活化人内分泌细胞系BON 中MEK/ERK/c-Jun通路,能够增加神经降压素分泌和基因表达。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 MkHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\oc29KSzVyPUCuNFAxOzB4IN88US=> MoezQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
OCUB-M NH3zZYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MljsTWM2OD1yLkCwNVk1KM7:TR?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
DOHH-2 NF\Re|RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlPrTWM2OD1yLkCwNlI2KM7:TR?= MnvwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
NOS-1 Mn3RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTBwMECyOFIh|ryP NWrTXmtqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
L-363 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGfvUW5KSzVyPUCuNFAzQDRizszN M3vvWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
RL95-2 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWq0[483UUN3ME2wMlAxOzJizszN NFjjc|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
BL-70 M3LEfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoqyTWM2OD1yLkCwN|M4KM7:TR?= MkDIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
TGBC1TKB MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUHJR|UxRTBwMECzO|kh|ryP MnLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
697 MkjvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXqO2xtUUN3ME2wMlAxPjN2IN88US=> M3XpPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
RPMI-8226 NGrJO5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4W4T2lEPTB;MD6wNFY2OSEQvF2= NYTDT5lkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
BL-41 NEG3RmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHKN3BGUUN3ME2wMlAxPjl4IN88US=> NVjKcIZSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
MRK-nu-1 NUnpd2N6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYfJR|UxRTBwMEC3NFch|ryP MkCxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
EW-1 NVTGbZJIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYnTcGV6UUN3ME2wMlAxPzR7IN88US=> MnfSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
EVSA-T NX;TPGN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml33TWM2OD1yLkCwPFkzKM7:TR?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
HUTU-80 NYrHXWlQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2D1c2lEPTB;MD6wNFkzQSEQvF2= NYjzOZZVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
VA-ES-BJ MnnmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jYeGlEPTB;MD6wNFk5PCEQvF2= NH7nbnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
GCIY M4DhfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrlcXY3UUN3ME2wMlAyODB5IN88US=> NX3KVXI{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
ES6 NFfObmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYLJR|UxRTBwMEGwPFMh|ryP NEDOXGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
NALM-6 M3\JT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkHrTWM2OD1yLkCxNVAzKM7:TR?= NXy4NnU3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
LB996-RCC M{f3SWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX7JR|UxRTBwMEGxN|ch|ryP MnLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
EW-16 NVHHNmRWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYLRfGlFUUN3ME2wMlAyOTl{IN88US=> M4q1SlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
KALS-1 NWHwWm84T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU[wUmQ6UUN3ME2wMlAyOTl6IN88US=> NETPT5c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
OPM-2 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NU[0WYRvUUN3ME2wMlAyOjlizszN MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
KU812 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPObopKSzVyPUCuNFEzQTZizszN M2rRV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
A4-Fuk M3GxN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzpfpRLUUN3ME2wMlAyOzF7IN88US=> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
KGN Mm[zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWn0W3JqUUN3ME2wMlAyOzV7IN88US=> M{\ZVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
KS-1 M4ntTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnvjTWM2OD1yLkCxN|kzKM7:TR?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
no-11 MlrQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojxTWM2OD1yLkCxN|k2KM7:TR?= M3PB[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
PSN1 M4HmZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NG\5WYFKSzVyPUCuNFE1ODlizszN NWm0fII3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
SK-LMS-1 M4DtOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfXPVZ3UUN3ME2wMlAyPDF|IN88US=> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
LS-411N M3;NR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEf6dmRKSzVyPUCuNFE1OzlizszN MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
HT-144 MmjWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnzmTWM2OD1yLkCxOFUyKM7:TR?= MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
ACN MkPSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUTJR|UxRTBwMEG1JO69VQ>? NUj5[oI3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
RKO M2XBemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFzKXYlKSzVyPUCuNFE2OzNizszN M3jJVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
CMK MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn7STWM2OD1yLkCxOVQ{KM7:TR?= NV;OSHdFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
GB-1 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1PTSGlEPTB;MD6wNVU2OyEQvF2= NHf6UpE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
KE-37 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIDscYFKSzVyPUCuNFE2PzNizszN NHO2NIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
no-10 M17n[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MY\JR|UxRTBwMEG1PFQh|ryP MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
MOLT-16 NIDxcHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\4TWM2OD1yLkCxOlIzKM7:TR?= MojZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
HEL MoPnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHFTWM2OD1yLkCxOlQh|ryP MnHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
NCI-H1355 NEXpUIRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml\wTWM2OD1yLkCxOlQ3KM7:TR?= MkjsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
IST-MEL1 NVjHToFRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\IfGlEPTB;MD6wNVc4PCEQvF2= NUHKZ|VpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
MHH-PREB-1 MmTPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DYeGlEPTB;MD6wNVgyQCEQvF2= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
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LC-2-ad NIDSR3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITU[HdKSzVyPUCuNFIyOzdizszN MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
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SW872 M3W4SGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4L3OWlEPTB;MD6wNlI1PyEQvF2= M2W5fVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
SK-UT-1 NIXsUnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUXLcpU2UUN3ME2wMlAzOjZzIN88US=> MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
OS-RC-2 NYDvZ|MzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3Tn[WlEPTB;MD6wNlMzOyEQvF2= NHvJUVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
NCI-H747 NUnpSZRrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUG0fGU6UUN3ME2wMlAzOzNizszN MonwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
MZ2-MEL M3S5ZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX3JR|UxRTBwMEKzN|ch|ryP NG\FbWw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
TE-11 MmXNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\pclJ6UUN3ME2wMlAzOzd{IN88US=> NXfLTWw4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
KURAMOCHI NWTGXo5nT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjPR|hKSzVyPUCuNFI1OTRizszN NUnVZVRkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
HD-MY-Z MmHUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHjZSJpKSzVyPUCuNFI1OjZizszN NHjBepo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
GI-1 Ml\QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVfJR|UxRTBwMEK0Olkh|ryP MkXJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
LB1047-RCC M1zpWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\ZeXBMUUN3ME2wMlAzPDh6IN88US=> M1[4NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
TE-12 M4LlXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYDJR|UxRTBwMEK1OUDPxE1? M2qxV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
MZ7-mel MmLpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHLEco5KSzVyPUCuNFI3ODlizszN M2P4TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
MMAC-SF MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkP6TWM2OD1yLkCyOlM{KM7:TR?= NUPSSmY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
A101D MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHTaXVFKSzVyPUCuNFI3PSEQvF2= M4HwSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
GR-ST NH7tfopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTBwMEK2OVUh|ryP NHz3bok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
MC116 M4T3Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;WbWp2UUN3ME2wMlAzPjhizszN NH;mWHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
Ramos-2G6-4C10 NV7CUFh[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXrTWM2OD1yLkCyO|Uh|ryP NEXRT|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
ATN-1 NGntXWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTBwMEK3PFkh|ryP MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
MFH-ino MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jtd2lEPTB;MD6wNlgyOiEQvF2= MmHCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
DEL MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUXJR|UxRTBwMEK4NVUh|ryP MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
OCI-AML2 MonyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XNcmlEPTB;MD6wNlg4OSEQvF2= M1qwWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
LXF-289 NWH2Rm9ST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7DRm1KSzVyPUCuNFI5PzJizszN NV35PVVPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
IA-LM Mlm3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTBwMEK5NVch|ryP NWrOc5RpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
EoL-1-cell MmrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfkUVJKSzVyPUCuNFI6QTZizszN MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
OVCAR-4 M{XD[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTBwMEOwPVQh|ryP NYH1PIJRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
ES1 MlPkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTBwMEOwPVch|ryP Mn7UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
CESS NIHZTJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTBwMEOwPVkh|ryP M2fTflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
GAK NX3SWWlrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3;VfmlEPTB;MD6wN|E5PiEQvF2= NGPlU|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
NTERA-S-cl-D1 MoP2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTBwMEOyNlYh|ryP MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
TE-10 NHrXSGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvKTnhNUUN3ME2wMlA{OzFzIN88US=> NXjuWGxLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
CCRF-CEM M13RRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NI\lV2dKSzVyPUCuNFM{PzFizszN M1fCOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
NMC-G1 M4X5PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoLCTWM2OD1yLkCzOFQ5KM7:TR?= M{nIZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
HOP-62 M2HXTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUi3cFJVUUN3ME2wMlA{PTV4IN88US=> NWfHN4xzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
SH-4 NYnWUlVGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3PSVGlEPTB;MD6wN|U5OiEQvF2= NHrVU449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
NCI-H1648 NYPLfINJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkTOTWM2OD1yLkCzOlIzKM7:TR?= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
EB-3 NUS4c2J7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvjVIVtUUN3ME2wMlA{PjR|IN88US=> MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
BB65-RCC NYTEcI1IT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX71[HRmUUN3ME2wMlA{PjlzIN88US=> NWG3Z3V7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
BE-13 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVPJR|UxRTBwMEO3OVkh|ryP MkTFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
CP67-MEL MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWHJR|UxRTBwMEO3OVkh|ryP NYXxXWplRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
SW954 M1j3[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHH5fpZKSzVyPUCuNFM4QSEQvF2= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
TE-5 NFvSRnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlTyTWM2OD1yLkCzPFU1KM7:TR?= M4DufFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
HCE-4 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3tV|ZQUUN3ME2wMlA{QDV3IN88US=> NH\2VHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
LC-1F M2LWS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHnCdHdKSzVyPUCuNFM6QDlizszN MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
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EW-11 NUTBUmtCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWDJR|UxRTJ2LkK3NVgh|ryP M{PWflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
EB2 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXvTWM2OD1|MT61N|E5KM7:TR?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
D-502MG NUi4SlJNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1vh[WlEPTB;NEOuPVIzQSEQvF2= M4jCc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
U-698-M MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DXcGlEPTB;NEiuPFY4OSEQvF2= NGTPc|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
ES3 NXj3RmRQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHH6W2NKSzVyPUS5MlM5QTNizszN NVHPVXpzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
PC3 MUfGeY5kfGmxbjDhd5NigQ>? NVzhU2d2UW6qaXLpeIlwdiCxZjDQTVNM[WyyaHGgbY4hcHWvYX6gVGM{KGOnbHzzJIV5eHKnc4PpcochSWu2MTDTOFc{TCCvdYThcpQh[XO|ZYPz[YQh[XNicHjvd5Bpd3K7bHH0bY9vKG:oIFHreEBVcHJ|MEigZpkhcW2vdX7vZoxwfHSrbnesJGVEPTBiPTCxMlgh|ryPLh?= NYfBW3E6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzNlI2PjZpPkKxN|IzPTZ4PD;hQi=>
TC32 NVrFT405eUiWUzDhd5NigQ>? M3jpcJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCWQ{OyJINmdGy| MnjrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
A673 MVXxTHRUKGG|c3H5 M1\MXJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy| NXXZc3g3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
BT-37 MnK1dWhVWyCjc4PhfS=> NYO5SI5QeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz M13STlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 MnzCdWhVWyCjc4PhfS=> MkDzdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 MUHxTHRUKGG|c3H5 MXHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? Mmq4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SJ-GBM2 MVPxTHRUKGG|c3H5 NIHkXGdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1qtS2JOOiClZXzsdy=> NILUTIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC NYrzfVY6eUiWUzDhd5NigQ>? M{DRdJFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB-EBc1 MUTxTHRUKGG|c3H5 MXjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhVkJvRVLjNUBk\Wyucx?= NIi4VY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC NV3UcmlzeUiWUzDhd5NigQ>? MYPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTT{1PNU2FIHPlcIx{ MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
TC32 MVfxTHRUKGG|c3H5 NHLJWXZyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBVSzN{IHPlcIx{ MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
体内研究 Torin1在20 mg/kg剂量下,在U87MG异种移植模型中是有效的,并且在肿瘤和外周组织中对mTOR下游效应蛋白表现出良好的药效学抑制作用。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

mTORC1和 mTORC2体外激酶试验:

为产生可溶性mTORC1,HEK-293T细胞系,稳定表达N端FLAG标记的Raptor,使用水泡性口炎病毒G-假型病毒MSCV逆转录酶病毒产生。对于mTORC2,产生相似的HeLa细胞,能够稳定表达N端FLAG-标记的Protor-1。两个复合物通过在50 mm HEPES,pH 7.4,10 mm 焦磷酸钠,10 mm β-甘油磷酸钠,100 mm NaCl,2 mm EDTA,0.3% CHAPS中裂解细胞纯化。细胞在4 °C下裂解30分钟,不溶解部分通过以13,000 rpm微量离心10分钟移除。上清液与FLAG-M2单克隆抗体-琼脂糖培育1小时,然后用裂解缓冲液洗涤3次,用包含终浓度0.5 M NaCl的裂解缓冲液洗涤1次。纯化的mTORC1用100 μg/mL 3×FLAG多肽在50 mm HEPES,pH 7.4,100 mm NaCl中洗脱。将洗出液等分收集,储存在-80 °C。纯化底物S6K1 和 Akt1。激酶试验在30 °C,由激酶缓冲液(25 mm HEPES,pH 7.4,50 mm KCl,10 mm MgCl2,500 μm ATP)和150 ng 灭活的S6K1或Akt1底物组成的20 μL终体积混合物中进行。反应通过加入80 μL样品缓冲液停止,并沸煮5分钟。随后,样品通过SDS-PAGE和免疫印迹法分析。
细胞实验:[1]
- 合并
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4天
  • Method: 细胞活性通过CellTiter-Glo发光法细胞活性测定评估。在第0天,细胞以500细胞每孔接种到96孔板,并生长过夜。第1天,细胞用适当的化合物处理,随后在第3-5天分析。对于分析,板在室温下培育60分钟,将50 μL CellTiter-Glo试剂加入每孔中,板在定轨摇床上混合12分钟。发光在标准平板光度计上定量。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: U87MG 异种移植物模型
  • Dosages: 20 mg/kg
  • Administration: 腹腔注射给药,每天一次
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 2 mg/mL warmed (3.29 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 607.62
化学式

C35H28F3N5O2

CAS号 1222998-36-8
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How to re-constitute the inhibitor for i.p. administration?

  • 回答:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

相关mTOR产品

Tags: 购买Torin 1 | Torin 1供应商 | 采购Torin 1 | Torin 1价格 | Torin 1生产 | 订购Torin 1 | Torin 1代理商
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID