Torin 1

For research use only. Not for use in humans.

目录号:S2827

Torin 1 Chemical Structure

CAS No. 1222998-36-8

Torin 1是一种有效的mTORC1/2抑制剂,在无细胞试验中IC50为2 nM/10 nM;作用于mTOR比作用于PI3K选择性高1000倍。

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RMB 1419.84 现货
RMB 3019.04 现货
RMB 5492.28 现货
RMB 15315.3 现货
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客户使用Selleck生产的Torin 1发表文献149篇:

产品安全说明书

mTOR抑制剂选择性比较

生物活性

产品描述 Torin 1是一种有效的mTORC1/2抑制剂,在无细胞试验中IC50为2 nM/10 nM;作用于mTOR比作用于PI3K选择性高1000倍。
靶点
mTORC1 [1]
(Cell-free assay)
mTOR [2]
(Cell-free assay)
DNA-PK [2]
(Cell-free assay)
mTORC2 [1]
(Cell-free assay)
p110γ [2]
(Cell-free assay)
2 nM 4.32 nM 6.34 nM 10 nM 171 nM
体外研究

Torin1在2和10 nM浓度下分别抑制mTORC1 和mTORC2底物的磷酸化。此外,Torin1对mTOR比对PI3K (EC50 = 1800 nM)的选择性高1000倍,比对450种其它蛋白激酶的结合选择性高100倍。[1] [2] Torin1通过耐rapamycin机制引起细胞周期阻滞,并且其不依赖于mTORC2。Torin1比rapamycin更完全地干扰mTORC1依赖表现型。Cap依赖性翻译需要mTORC1耐Rapamycin的功能。[1]在近期的一项研究中,据报道Torin1通过活化人内分泌细胞系BON 中MEK/ERK/c-Jun通路,能够增加神经降压素分泌和基因表达。[3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
ETK-1 M17pS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlzJTWM2OD1yLkCwNFMxPiEQvF2= NEThd|A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
OCUB-M NVWzVpJ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvsZ3dxUUN3ME2wMlAxOTl2IN88US=> NY\zbINLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
DOHH-2 M{L5RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmLhTWM2OD1yLkCwNlI2KM7:TR?= NWfo[3Q4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
NOS-1 MknHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfzVpk2UUN3ME2wMlAxOjR{IN88US=> NIPqXFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
L-363 NXXzfYJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn\PTWM2OD1yLkCwNlg1KM7:TR?= NEm3ZmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
RL95-2 NYHCe3FDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYraUnJoUUN3ME2wMlAxOzJizszN NUDQTHRnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
BL-70 NFvyXZZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlf1TWM2OD1yLkCwN|M4KM7:TR?= NF7YVm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
TGBC1TKB MmHTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF3CV5pKSzVyPUCuNFA{PzlizszN MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
697 Mnr0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEfDRVFKSzVyPUCuNFA3OzRizszN MmG4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
RPMI-8226 M4D4W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkG0TWM2OD1yLkCwOlUyKM7:TR?= NIDFdWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
BL-41 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUnJR|UxRTBwMEC2PVYh|ryP M{i2TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
MRK-nu-1 NEPyc3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDzTWM2OD1yLkCwO|A4KM7:TR?= Mn;1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
EW-1 NV64[GhoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmC4TWM2OD1yLkCwO|Q6KM7:TR?= MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
EVSA-T MkjIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTBwMEC4PVIh|ryP MlfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
HUTU-80 NWixbFRGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYnJR|UxRTBwMEC5Nlkh|ryP NI\2PYk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
VA-ES-BJ M1TqZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWTReJo{UUN3ME2wMlAxQTh2IN88US=> NXLne3pnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
GCIY M2HVZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV3JR|UxRTBwMEGwNFch|ryP MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
ES6 M1XJTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nmXmlEPTB;MD6wNVA5OyEQvF2= NEX3WJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
NALM-6 NYrIOo5lT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDXdWxsUUN3ME2wMlAyOTB{IN88US=> NIrCcVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
LB996-RCC MmTQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\abWNxUUN3ME2wMlAyOTN5IN88US=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
EW-16 NIXReFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\TSmlEPTB;MD6wNVE6OiEQvF2= MlTyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
KALS-1 NGrlWmxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYjJR|UxRTBwMEGxPVgh|ryP MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
OPM-2 NYjQZoFYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVrtN|RFUUN3ME2wMlAyOjlizszN M1PSOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
KU812 NXzqTXh4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJR|UxRTBwMEGyPVYh|ryP MlHUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
A4-Fuk Mn3pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLjVIxKSzVyPUCuNFE{OTlizszN NXK0e5pNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
KGN M2HGV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGfTRZBKSzVyPUCuNFE{PTlizszN Mm\pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
KS-1 NF\PZnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjuT45kUUN3ME2wMlAyOzl{IN88US=> NHTxO3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
no-11 Mn7XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHRNVRKSzVyPUCuNFE{QTVizszN NUHCb21zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
PSN1 M3vj[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFrRUWdKSzVyPUCuNFE1ODlizszN NIjBdXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
SK-LMS-1 NICxXmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfFfldQUUN3ME2wMlAyPDF|IN88US=> NIjWcpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
LS-411N MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnYOm5KSzVyPUCuNFE1OzlizszN NGnEUm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
HT-144 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2S0b2lEPTB;MD6wNVQ2OSEQvF2= NELUc5E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
ACN NYfhOFNFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnnLTWM2OD1yLkCxOUDPxE1? NFnOWFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
RKO NECzeFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3zMWWlEPTB;MD6wNVU{OyEQvF2= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
CMK Ml3SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXnobZdxUUN3ME2wMlAyPTR|IN88US=> NVPTZpdJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
GB-1 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXrJR|UxRTBwMEG1OVMh|ryP NIm0XIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
KE-37 NG\rUm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHBOWs1UUN3ME2wMlAyPTd|IN88US=> NYO1eoQxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
no-10 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTLTWM2OD1yLkCxOVg1KM7:TR?= NXSxT4ZHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
MOLT-16 NHLmR|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFL5VnhKSzVyPUCuNFE3OjJizszN MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
HEL M1;4R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlfHTWM2OD1yLkCxOlQh|ryP M3i1RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
NCI-H1355 MlTSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPsbmZLUUN3ME2wMlAyPjR4IN88US=> M{fxU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
IST-MEL1 NFK5SVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPtTWM2OD1yLkCxO|c1KM7:TR?= NIf5TYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
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ES8 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTGTWM2OD1yLkCxPFQ1KM7:TR?= NFT0RXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
LC-2-ad M{nrOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoXhTWM2OD1yLkCyNVM4KM7:TR?= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
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TE-11 NVT6PHZZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUn2[FQyUUN3ME2wMlAzOzd{IN88US=> M1\JXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
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LB1047-RCC Mn\6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmW5TWM2OD1yLkCyOFg5KM7:TR?= M3HVd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
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MMAC-SF NXnpR286T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlXuTWM2OD1yLkCyOlM{KM7:TR?= NFnEXoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
A101D MojxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPwTWM2OD1yLkCyOlUh|ryP MlTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
GR-ST NXr3T|N[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTBwMEK2OVUh|ryP M2SzUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
MC116 M33aS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vicWlEPTB;MD6wNlY5KM7:TR?= NYHVUWdWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
Ramos-2G6-4C10 NYjZd|BbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHzYOmNKSzVyPUCuNFI4PSEQvF2= NHT3XYg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
ATN-1 NXnLSFIyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7ie|VKSzVyPUCuNFI4QDlizszN NH7JRlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
MFH-ino MlvmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXuTWM2OD1yLkCyPFEzKM7:TR?= NYr1[|NsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
DEL MkLmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTBwMEK4NVUh|ryP MlTOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
OCI-AML2 NEO2VY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDwTWM2OD1yLkCyPFcyKM7:TR?= NHfl[ZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
LXF-289 NHTGeWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3LlemlEPTB;MD6wNlg4OiEQvF2= Mlv1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
IA-LM M{niOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn3aTWM2OD1yLkCyPVE4KM7:TR?= M2LYbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
EoL-1-cell MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTBwMEK5PVYh|ryP MlLYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
OVCAR-4 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYnBN|BGUUN3ME2wMlA{ODl2IN88US=> MknUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
ES1 MoXDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPTRnhnUUN3ME2wMlA{ODl5IN88US=> NU\KTXZkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
CESS MnzuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrQTWM2OD1yLkCzNFk6KM7:TR?= MojyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
GAK MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTBwMEOxPFYh|ryP MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
NTERA-S-cl-D1 MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUnoPHloUUN3ME2wMlA{OjJ4IN88US=> MkLyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
TE-10 NGH3doJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUPJR|UxRTBwMEOzNVEh|ryP NH;0Vmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
CCRF-CEM NX2wU4FWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV\JR|UxRTBwMEOzO|Eh|ryP NWDqNY1ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
NMC-G1 NWSzW3N7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlTxTWM2OD1yLkCzOFQ5KM7:TR?= MnvOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
HOP-62 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXNTWM2OD1yLkCzOVU3KM7:TR?= MlHSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
SH-4 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnT0TWM2OD1yLkCzOVgzKM7:TR?= MkLOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
NCI-H1648 NWjIc2xKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvxNldbUUN3ME2wMlA{PjJ{IN88US=> M1zOSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
EB-3 NHr6SFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF70SGlKSzVyPUCuNFM3PDNizszN NF3PSJc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
BB65-RCC NWK0NpNET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1P0VmlEPTB;MD6wN|Y6OSEQvF2= NX61RWQ2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTJ3NkS1PU8oRlODTlfFVlww[T5?
BE-13 M1nDVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NETuVHpKSzVyPUCuNFM4PTlizszN MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
CP67-MEL M{DOVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUO2d3V3UUN3ME2wMlA{PzV7IN88US=> NEXSRVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
SW954 MnLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHBTWM2OD1yLkCzO|kh|ryP MlzuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
TE-5 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWL1VoxwUUN3ME2wMlA{QDV2IN88US=> M37rN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
HCE-4 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\NNXZKSzVyPUCuNFM5PTVizszN M2DRVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFEzPTZ2NUmvK|5USU6JRWK8M4E,
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EW-11 NUTzTplTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlyxTWM2OD1{ND6yO|E5KM7:TR?= MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
EB2 NV\xRpp7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjrTWM2OD1|MT61N|E5KM7:TR?= MojnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNVI2PjR3OT:nQnNCVkeHUkyvZV4>
D-502MG MkmzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTR|LkmyNlkh|ryP NEfMUVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
U-698-M NGPuRZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYm3OXkxUUN3ME20PE45PjdzIN88US=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyxNlU3PDV7Lze+V2FPT0WUPD;hQi=>
ES3 NX7UfphPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3L3fWlEPTB;NEmuN|g6OyEQvF2= NIrPXGE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyOjV4NEW5M{c,W0GQR1XSQE9iRg>?
PC3 NXvQT2tPTnWwY4Tpc44h[XO|YYm= NEXNVFZKdmirYnn0bY9vKG:oIGDJN2tidHCqYTDpckBpfW2jbjDQR|Mh[2WubIOg[ZhxemW|c3nu[{BCc3RzIGO0O|NFKG23dHHueEBie3Onc4Pl[EBieyCyaH;zdIhwenmuYYTpc44hd2ZiQXv0JHRpejNyODDifUBqdW23bn;icI91fGmwZzygSWM2OCB;IEGuPEDPxE1w NXTKfYIyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGzNlI2PjZpPkKxN|IzPTZ4PD;hQi=>
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A673 MXnxTHRUKGG|c3H5 M1zFZZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCDNkezJINmdGy| Mme5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
BT-37 MkfrdWhVWyCjc4PhfS=> NFXJb5JyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQmStN|ch[2WubIO= M1TuTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
OHS-50 M{fGU5FJXFNiYYPzZZk> Mlm0dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE:KUz21NEBk\Wyucx?= NGPiSYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Rh41 M{jFUpFJXFNiYYPzZZk> NUj3XIFqeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGLoOFEh[2WubIO= NUHrd2FGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SJ-GBM2 M1ezTJFJXFNiYYPzZZk> NVvMSYZneUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPKMWdDVTJiY3XscJM> NF3ieIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SK-N-MC NIP0UnVyUFSVIHHzd4F6 Ml7idWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKFONLV6tUWMh[2WubIO= M2PIeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1 NWLzXWNJeUiWUzDhd5NigQ>? M1rmfpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCQQj3FRoMyKGOnbHzz M3LheFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC M17uUJFJXFNiYYPzZZk> NH\vWYVyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLV3DJINmdGy| MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
TC32 NGTlblNyUFSVIHHzd4F6 MknNdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsdy=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b; 

PubMed: 28494858     


4E-BPs are required for mTOR control of ATF4. 4E-BP1/2 wild-type (WT), double knockout (DKO), or DKO MEFs stably expressing 4ebp1 (DKO + 4ebp1) were treated with vehicle (DMSO) or 250 nM Torin 1 for 4 hr. Cell extracts were analyzed by immunoblotting for the indicated proteins.

LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236); 

PubMed: 24126619     


Cells were treated with increasing doses of the mTOR inhibitors, torin 1 (A) and rapamycin (B), in RPMI-1640 containing 10% FBS for 2 days. LC3 processing, p62 accumulation, PARP cleavage, AKT phosphorylation and mTOR pathway activity (indicated by phosphorylation of 4E-BP, S6K1 and S6) were analyzed by western blotting of total cell lysates. GAPDH was the control for protein loading.

p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1; 

PubMed: 21965657     


mTOR kinase activity controls abundance of SIN1. MDA-MB-435 cells were incubated with specific mTOR inhibitor (0.5 μm TORIN) for 24 or 48 h and analyzed.

Vinculin / p-S6; 

PubMed: 28757207     


A375 cells were treated with Torin1 for 48 h and localization of proteins was visualized by confocal microscopy. Scale bar, 50 μm. Focal adhesion size was measured by Fiji program. 

28494858 24126619 21965657 28757207
Immunofluorescence
lipin-1; 

PubMed: 28347999     


Inhibition of mTOR activity promoted the nuclear translocation of lipin-1. After treatment with or without torin 1 for 4 h, cells expressing HA-lipin-1 were fixed and stained with a rabbit HA antibody followed by Alexa 594–conjugated goat anti-rabbit secondary antibody. Nuclei were stained with DAPI. Scale bars, 20 μM.

28347999
Growth inhibition assay
Cell viability; 

PubMed: 28757207     


Cancer cell lines were grown in complete media with/without mTOR inhibitor, Torin1 (250 nM). Media and Torin1 were replaced every 2 days and cells were counted at the indicated time points. Data are the means ± SD of three separate experiments performed in triplicate.

28757207
体内研究 Torin1在20 mg/kg剂量下,在U87MG异种移植模型中是有效的,并且在肿瘤和外周组织中对mTOR下游效应蛋白表现出良好的药效学抑制作用。[2]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[1]
- 合并

mTORC1和 mTORC2体外激酶试验:

为产生可溶性mTORC1,HEK-293T细胞系,稳定表达N端FLAG标记的Raptor,使用水泡性口炎病毒G-假型病毒MSCV逆转录酶病毒产生。对于mTORC2,产生相似的HeLa细胞,能够稳定表达N端FLAG-标记的Protor-1。两个复合物通过在50 mm HEPES,pH 7.4,10 mm 焦磷酸钠,10 mm β-甘油磷酸钠,100 mm NaCl,2 mm EDTA,0.3% CHAPS中裂解细胞纯化。细胞在4 °C下裂解30分钟,不溶解部分通过以13,000 rpm微量离心10分钟移除。上清液与FLAG-M2单克隆抗体-琼脂糖培育1小时,然后用裂解缓冲液洗涤3次,用包含终浓度0.5 M NaCl的裂解缓冲液洗涤1次。纯化的mTORC1用100 μg/mL 3×FLAG多肽在50 mm HEPES,pH 7.4,100 mm NaCl中洗脱。将洗出液等分收集,储存在-80 °C。纯化底物S6K1 和 Akt1。激酶试验在30 °C,由激酶缓冲液(25 mm HEPES,pH 7.4,50 mm KCl,10 mm MgCl2,500 μm ATP)和150 ng 灭活的S6K1或Akt1底物组成的20 μL终体积混合物中进行。反应通过加入80 μL样品缓冲液停止,并沸煮5分钟。随后,样品通过SDS-PAGE和免疫印迹法分析。
细胞实验:[1]
- 合并
  • Cell lines: MEFs
  • Concentrations: ~250 nM
  • Incubation Time: 4天
  • Method: 细胞活性通过CellTiter-Glo发光法细胞活性测定评估。在第0天,细胞以500细胞每孔接种到96孔板,并生长过夜。第1天,细胞用适当的化合物处理,随后在第3-5天分析。对于分析,板在室温下培育60分钟,将50 μL CellTiter-Glo试剂加入每孔中,板在定轨摇床上混合12分钟。发光在标准平板光度计上定量。
    (Only for Reference)
动物实验:[2]
- 合并
  • Animal Models: U87MG 异种移植物模型
  • Dosages: 20 mg/kg
  • Administration: 腹腔注射给药,每天一次
    (Only for Reference)

溶解度 (25°C)

体外 DMSO 2 mg/mL warmed (3.29 mM)
Water Insoluble
Ethanol Insoluble

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 607.62
化学式

C35H28F3N5O2

CAS号 1222998-36-8
储存条件 粉状
溶于溶剂
别名 N/A

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % ddH2O
计算重置

计算器

摩尔浓度计算器

摩尔浓度计算器

本计算器可帮助您计算出特定溶液中溶质的质量、溶液浓度和体积之间的关系,公式为:

质量 (mg) = 浓度 (mM) x 体积 (mL) x 分子量 (g/mol)

摩尔浓度计算公式

  • 质量
    浓度
    体积
    分子量

*在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。

稀释计算器

稀释计算器

用本工具协助配置特定浓度的溶液,使用的计算公式为:

开始浓度 x 开始体积 = 最终浓度 x 最终体积

稀释公式

稀释公式一般简略地表示为: C1V1 = C2V2 ( 输入 输出 )

  • C1
    V1
    C2
    V2

在配置溶液时,请务必参考Selleck产品标签上、SDS / COA(可在Selleck的产品页面获得)批次特异的分子量使用本工具。.

连续稀释计算器方程

  • 连续稀释

  • 计算结果

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
分子量计算器

分子量计算器

通过输入化合物的化学式来计算其分子量:

总分子量:g/mol

注:化学分子式大小写敏感。C10H16N2O2 c10h16n2o2

摩尔浓度计算器

质量 浓度 体积 分子量
计算

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

  • * 必填项

常见问题及建议解决方法

  • 问题 1:

    How to re-constitute the inhibitor for i.p. administration?

  • 回答:

    S2827 Torin 1 can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.

mTOR Signaling Pathway Map

mTOR Inhibitors with Unique Features

相关mTOR产品

Tags: 购买Torin 1 | Torin 1供应商 | 采购Torin 1 | Torin 1价格 | Torin 1生产 | 订购Torin 1 | Torin 1代理商
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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID