AZD1480

目录号:S2162

仅限科研使用

AZD1480是一种新型,ATP竞争性的JAK2抑制剂,无细胞试验中IC50为0.26 nM,选择性作用于JAK3和Tyk2,对JAK1的作用较弱。Phase 1。

AZD1480 Chemical Structure

CAS: 935666-88-9

规格 价格 库存 购买数量
10mM (1mL in DMSO) RMB 1071.19 现货
RMB 819.48 现货
RMB 4671.71 现货
大包装 有超大折扣

今日订购,明日送达 全国免运费 分装免费 分装成小规格产品(单支最小为1mg)
如1支10mg产品可分装为10支1mg产品

全国免费电话:400-668-6834   |   Email:info@selleck.cn

客户使用Selleck生产的AZD1480发表文献101篇:

产品安全说明书

JAK抑制剂选择性比较

相关JAK产品

生物活性

产品描述 AZD1480是一种新型,ATP竞争性的JAK2抑制剂,无细胞试验中IC50为0.26 nM,选择性作用于JAK3和Tyk2,对JAK1的作用较弱。Phase 1。
特性 AZD1480为治疗多发性骨髓瘤的潜在新型治疗剂。
靶点
JAK2 [1]
(Cell-free assay)
0.26 nM
体外研究

5μM AZD1480诱导细胞周期停在G2/M期,且通过抑制Aurora激酶而诱导细胞死亡。[1]AZD1480是JAK2的有效抑制剂,作用于人类多发性骨髓瘤细胞,可以抑制生长,存活,FGFR3 和 STAT3信号,及下游靶点,包括 Cyclin D2。在低微摩尔浓度时,AZD1480抑制细胞增殖和诱导骨髓瘤细胞系凋亡。[2]AZD1480 作用于人和鼠神经胶质瘤细胞,有效抑制组成型和诱导型JAK1, JAK2, 及STAT-3的磷酸化作用 ,结果导致细胞增殖降低,且诱导凋亡。[3]AZD1480是有效的ATP竞争性的JAK1/2激酶小分子抑制剂,可抑制STAT3磷酸化和肿瘤生长,这种抑制存在STAT3依赖性。AZD1480通过影响肿瘤的微环境而抑制肿瘤血管生成和局部转移。[4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SW620 NWLCO2RiTnWwY4Tpc44hSXO|YYm= MmnwOUDPxE1? NFfEVYE1QCCq MlTQSG1UVw>? NUD0WG5Z[myxY3vzJGpCUzJxU2TBWFMhe2mpbnHsbY5o NYjwbopwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW5OVQ6PzRpPkK1PVU1QTd2PD;hQi=>
LoVo  M{W3PWZ2dmO2aX;uJGF{e2G7 MkHkOUDPxE1? NWP5Xm85PDhiaB?= NEHlWnhFVVOR NHLFfXRjdG:la4OgTmFMOi:VVFHUN{B{cWewYXzpcoc> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTl3NEm3OEc,OjV7NUS5O|Q9N2F-
HN5 NXjad5BGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2P0c|czKGh? NGKzfoRGSzVyPUOuPFEhyrFiMT65PUDPxE1? M4qzNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEGwNFExLz5{NUixNFAyODxxYU6=
Cal33 MlTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXG3NkBp NV;oWYV4TUN3ME2zMlM4KMLzIECuO|Uh|ryP MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPThzMECxNEc,OjV6MUCwNVA9N2F-
UM-22B NVLFTpJST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MknGO|IhcA>? MmH4SWM2OD1{Lk[2JOKyKDBwMkSg{txO NHTsTZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUixNFAyOCd-MkW4NVAxOTB:L3G+
686LN MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnwOIZrPzJiaB?= NUDQbmFzTUN3ME2yMlA2KMLzIEGuN|Mh|ryP MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPThzMECxNEc,OjV6MUCwNVA9N2F-
UM SCC-1 MkDBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmHRO|IhcA>? MVvFR|UxRTFwNkegxtEhOC52MjFOwG0> MlrmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MUCwNVAoRjJ3OEGwNFExRC:jPh?=
UM-22A M1vrTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4npfFczKGh? NH[4bXdGSzVyPUGuN|IhyrFiMD6zPUDPxE1? Mn\SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV6MUCwNVAoRjJ3OEGwNFExRC:jPh?=
OSC19 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVW3NkBp Ml73SWM2OD1zLkK2JOKyKDBwMkCg{txO NHXUdIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUixNFAyOCd-MkW4NVAxOTB:L3G+
PCI-52 NVm5WoJpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHHZYxNPzJiaB?= MmT0SWM2OD1zLkCwJOKyKDBwMEmg{txO MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPThzMECxNEc,OjV6MUCwNVA9N2F-
PCI-15B Mk\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPNc21wPzJiaB?= NFyxe3VGSzVyPUCuPVkhyrFiMT63OEDPxE1? NEK0SW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUixNFAyOCd-MkW4NVAxOTB:L3G+
UMSCC-1 NH;KR5NHfW6ldHnvckBCe3OjeR?= NHTnPVkxNjByMEWtNU43KM7:TR?= NH7wR5czPCCq NGfNTZFi[nKxZ3H0[ZMhUUxvNvMAl4lv\HWlZXSgeZAuemWpdXzheIlwdiCxZjDwV3RCXDOWeYK3NFXDqGmwIHGg[I9{\S2mZYDlcoRmdnRibXHucoVz NYrlUIl1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NVAxOTBpPkK1PFExODFyPD;hQi=>
Cal33 MmHQSpVv[3Srb36gRZN{[Xl? MYSwMlAxODVvMz64JO69VQ>? M2jHTFI1KGh? MVLhZpJw\2G2ZYOgTWwuPuLCk3nu[JVk\WRidYCtdoVofWyjdHnvckBw\iCyU2TBWFNVgXJ5MEZCpIlvKGFiZH;z[U1l\XCnbnTlcpQhdWGwbnXy M1HzSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3OEGwNFExLz5{NUixNFAyODxxYU6=
HH5 NG\sbYhHfW6ldHnvckBCe3OjeR?= MWWwMlAxODVvMz64JO69VQ>? M4O0RVI1KGh? MkC4ZYJzd2ejdHXzJGlNNTckgKPpcoR2[2WmIIXwMZJm\3WuYYTpc44hd2ZicGPURXQ{XHm{N{C1xsBqdiCjIHTvd4Uu\GWyZX7k[Y51KG2jbn7ldi=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPThzMECxNEc,OjV6MUCwNVA9N2F-
UM-22A NH\Ud4JHfW6ldHnvckBCe3OjeR?= NGPXNY0xNjByMEWtNU43KM7:TR?= M1O5blI1KGh? M{DlO4Fjem:pYYTld{BKVC144pETbY5lfWOnZDD1dE1z\We3bHH0bY9vKG:oIIDTWGFVO1S7ckewOeKhcW5iYTDkc5NmNWSncHXu[IVvfCCvYX7u[ZI> NWH0b4duRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW4NVAxOTBpPkK1PFExODFyPD;hQi=>
A1847 NXP5eWJETnWwY4Tpc44hSXO|YYm= NIjEXHIxNjB3LUGwJO69VQ>? MmDmNlQhcA>? NXjFR3cxTE2VT9Mg NGjGb|dz\WS3Y3XzJJBpd3OyaH;yfYxifGWmIGPURXQ{KGyndnXsdy=> M2j2NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkS2NFE2Lz5{NU[0OlAyPTxxYU6=
OVCAR-5 MYXGeY5kfGmxbjDBd5NigQ>? NHHFWHgxNjB3LUGwJO69VQ>? Ml3FNlQhcA>? MVfEUXNQyqB? NYnZb4w4emWmdXPld{BxcG:|cHjvdplt[XSnZDDTWGFVOyCuZY\lcJM> MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ2NkCxOUc,OjV4NE[wNVU9N2F-
OVCAR-8 MWrGeY5kfGmxbjDBd5NigQ>? MViwMlA2NTFyIN88US=> Mme4NlQhcA>? MVHEUXNQyqB? NFHNbo1z\WS3Y3XzJJBpd3OyaH;yfYxifGWmIGPURXQ{KGyndnXsdy=> MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ2NkCxOUc,OjV4NE[wNVU9N2F-
MOVCAR-5447 MUTGeY5kfGmxbjDBd5NigQ>? NVzz[2hiOC5yNT2xNEDPxE1? M{fSZVI1KGh? NFPYXGxFVVORwrC= NVHSWYdLemWmdXPld{BxcG:|cHjvdplt[XSnZDDTWGFVOyCuZY\lcJM> NWHxZZM3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2OFYxOTVpPkK1OlQ3ODF3PD;hQi=>
MOVCAR-5009 NHrVR4VHfW6ldHnvckBCe3OjeR?= NEjUWIYxNjB3LUGwJO69VQ>? MnTMNlQhcA>? NVL2TVFvTE2VT9Mg M2rUOpJm\HWlZYOgdIhwe3Cqb4L5cIF1\WRiU2TBWFMhdGW4ZXzz MojFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4NE[wNVUoRjJ3NkS2NFE2RC:jPh?=
A1847 NH;4TI5E\WyuIG\pZYJqdGm2eTDBd5NigQ>? MVSwMlA2NTFyIN88US=> NHjpXVY4OiCq NG\uOphFVVOR NH6zcGVz\WS3Y3XzJINmdGxidnnhZoltcXS7IHH0JJRp\SClb37j[ZJvfHKjdHnvckBw\iB3IN88US=> MlPmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4NE[wNVUoRjJ3NkS2NFE2RC:jPh?=
OVCAR-5 NVHJW4RPS2WubDDWbYFjcWyrdImgRZN{[Xl? NGD0fZIxNjB3LUGwJO69VQ>? MlraO|IhcA>? NVHQbWJPTE2VTx?= MlLxdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCjdDD0bIUh[2:wY3XycpRz[XSrb36gc4YhPSEQvF2= M{HMU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3NkS2NFE2Lz5{NU[0OlAyPTxxYU6=
OVCAR-8  MX7D[YxtKF[rYXLpcIl1gSCDc4PhfS=> M2jhWlAvODVvMUCg{txO NGrRNpI4OiCq MWTEUXNQ MkXxdoVlfWOnczDj[YxtKH[rYXLpcIl1gSCjdDD0bIUh[2:wY3XycpRz[XSrb36gc4YhPSEQvF2= MkTYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4NE[wNVUoRjJ3NkS2NFE2RC:jPh?=
OVCAR-5  MkizRZBweHSxc3nzJGF{e2G7 Mn6yNE42NzFxNTFOwG0> NYHnfodpPDhiaB?= NWDrVnpFTE2VTx?= NXvBN3FQcW6mdXPld{B{cWewaX\pZ4FvfCCjcH;weI9{cXNiYYSgbIlocCClb37j[ZJvfHKjdHnvci=> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ2NkCxOUc,OjV4NE[wNVU9N2F-
OVCAR-8  MV7BdI9xfG:|aYOgRZN{[Xl? NHPHeVAxNjVxMT:1JO69VQ>? MVu0PEBp M{TKfGROW09? NUjWSZFUcW6mdXPld{B{cWewaX\pZ4FvfCCjcH;weI9{cXNiYYSgbIlocCClb37j[ZJvfHKjdHnvci=> NXfmdpNNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2OFYxOTVpPkK1OlQ3ODF3PD;hQi=>
AKRSL MkfIR4VtdCCYaXHibYxqfHliQYPzZZk> MUS3NkBp MXrJR|Ux97zgMUCg{txO NVfoSXFHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW1NFQ3OzVpPkK1OVA1PjN3PD;hQi=>
PALJDL NFTUR3ZE\WyuIG\pZYJqdGm2eTDBd5NigQ>? NWWzd5ozPzJiaB?= NHy1RXVKSzVyPUKuOEDPxE1? Mn[xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3MES2N|UoRjJ3NUC0OlM2RC:jPh?=
MO4 M{TSWmZ2dmO2aX;uJGF{e2G7 MY[wMlUwOS93IN88US=> M2\rRVYhcA>? NFmwfndqdmirYnn0d{BRNVOWQWSzJIV5eHKnc4Ppc47DqA>? NXv4PXFqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWxOFk2OzVpPkK1NVQ6PTN3PD;hQi=>
DU145  NGXIeHJHfW6ldHnvckBCe3OjeR?= M1jKZlAuOjByIH7N NEe1TJEyKGkEoB?= MXjzeZBxemW|c3XzJGlNNTZvYXP0bZZifGWmIGP0ZZQ{KGGwZDDFVmsyNzJic3nncoFtcW6p MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV5N{m0Nkc,OjR3N{e5OFI9N2F-
DU145  NXL1U3A2TnWwY4Tpc44hSXO|YYm= MUe4NFAhdk1? M3\CXVczKGh? NYfmXZVpe3WycILld5NmeyCLTD22MYlv\HWlZXSgcYloemG2b4L5 M4mxRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUe3PVQzLz5{NEW3O|k1OjxxYU6=
CWR22Rv1 MoDJSpVv[3Srb36gRZN{[Xl? NYeyNJJYQDByIH7N NVrMS49iPzJiaB?= NFLjS4p{fXCycnXzd4V{KEmOLU[tbY5lfWOnZDDtbYdz[XSxcom= NID3[HU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEW3O|k1Oid-MkS1O|c6PDJ:L3G+
N592 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MonaTWM2OD1yLki0JO69VQ>? MlvTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNUi3NFEoRjJ2MUW4O|AyRC:jPh?=
H82 NHn1ZmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX;JR|UxRTFwM{eg{txO NHrhbZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1PFcxOSd-MkSxOVg4ODF:L3G+
GLC4 MmnpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIr4OlBKSzVyPUGuO|kh|ryP NFnWSZg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1PFcxOSd-MkSxOVg4ODF:L3G+
H526 NGXWXXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\5Z|B[UUN3ME2zMlA5KM7:TR?= NFr6PGI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1PFcxOSd-MkSxOVg4ODF:L3G+
H1173 MlnxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTJwM{mg{txO NXTYTIZtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVg4ODFpPkK0NVU5PzBzPD;hQi=>
DMS114 NXnYOJJST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUDJR|UxRTBwN{Og{txO MojnQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzNUi3NFEoRjJ2MUW4O|AyRC:jPh?=
NCI-N592 MmCySpVv[3Srb36gRZN{[Xl? NFW2PGExNjNxMT:zJO69VQ>? M3fjblI1KGh? NWDBOIJscW6mdXPld{BIOi:PIHPlcIwh[3mlbHWgZZJz\XO2 NF\wUpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1PFcxOSd-MkSxOVg4ODF:L3G+
GLC4 NX[2PJBiTnWwY4Tpc44hSXO|YYm= NUXTO3dnOC5|L{GvN{DPxE1? MX[yOEBp MlnabY5lfWOnczDHNk9OKGOnbHygZ5lkdGViYYLy[ZN1 M1\jSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUW4O|AyLz5{NEG1PFcxOTxxYU6=
NCI-H82 MVrGeY5kfGmxbjDBd5NigQ>? MoOyNE4{NzFxMzFOwG0> MmHaNlQhcA>? MXrpcoR2[2W|IFeyM20h[2WubDDjfYNt\SCjcoLld5Q> M2myeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUW4O|AyLz5{NEG1PFcxOTxxYU6=
NCI-N592 MlS5RZBweHSxc3nzJGF{e2G7 NETHdm0xNjNxMT:zJO69VQ>? NVvJbVROPDhiaB?= M{PvO4lv[3KnYYPld{B1cGVibHX2[Ywhd2ZiY3zlZZZm\C2FYYPwZZNmKDN? NITrV4I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1PFcxOSd-MkSxOVg4ODF:L3G+
GLC4 M3nhUGFxd3C2b4Ppd{BCe3OjeR?= MUmwMlMwOS9|IN88US=> NVvueJlTPDhiaB?= NHPHTGRqdmO{ZXHz[ZMhfGinIHzleoVtKG:oIHPs[YF3\WRvQ3HzdIF{\SB| NIrzWZE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG1PFcxOSd-MkSxOVg4ODF:L3G+
NCI-H82 NUXzfGxHSXCxcITvd4l{KEG|c3H5 MX[wMlMwOS9|IN88US=> NFXMeXo1QCCq MV;pcoNz\WG|ZYOgeIhmKGyndnXsJI9nKGOuZXH2[YQuS2G|cHHz[UA{ NXrMXGhoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxOVg4ODFpPkK0NVU5PzBzPD;hQi=>
CWR22Rv1  MVrBdI9xfG:|aYOgRZN{[Xl? Mnf3TWM2OD1yLkS4NkDPxE1? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl2MkC5OUc,OjN7NEKwPVU9N2F-
CWR22Pc MoS1RZBweHSxc3nzJGF{e2G7 NYn2Smo{UUN3ME2wMlQ{QCEQvF2= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzl2MkC5OUc,OjN7NEKwPVU9N2F-
PC-3 MVLBdI9xfG:|aYOgRZN{[Xl? NELhV5dKSzVyPUGuO|U2KM7:TR?= M4XsNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|OUSyNFk2Lz5{M{m0NlA6PTxxYU6=
DU145 M3rqbGFxd3C2b4Ppd{BCe3OjeR?= M3e1NGlEPTB;Mz61NVch|ryP NX7uNZlXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO5OFIxQTVpPkKzPVQzODl3PD;hQi=>
RC165N Mny5RZBweHSxc3nzJGF{e2G7 NVHTUI54UUN3ME2yMlA5OyEQvF2= NIGydnk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{m0NlA6PSd-MkO5OFIxQTV:L3G+
ARPE19 M{P2PGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzEUXNQ NVv2doNSUUN3ME2yOE4{QCEQvF2= MkPxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{G5NlEoRjJ|NUOxPVIyRC:jPh?=
HEK293 M1fOZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkXhSG1UVw>? MXPJR|UxRThwNkeg{txO MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV|MUmyNUc,OjN3M{G5NlE9N2F-
KCNR MnyxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoCwSG1UVw>? MUjJR|UxRTBwNE[g{txO MonlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{G5NlEoRjJ|NUOxPVIyRC:jPh?=
SY5Y MnP0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF6y[lhFVVOR Mk\qTWM2OD1yLkO2JO69VQ>? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV|MUmyNUc,OjN3M{G5NlE9N2F-
BE2 Mm\SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUmzdm1TTE2VTx?= NY\1[GhpUUN3ME2wMlcyKM7:TR?= MlHMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{G5NlEoRjJ|NUOxPVIyRC:jPh?=
AS M4HhdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUXEUXNQ MXjJR|UxRTFwNUOg{txO M1i5XVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUOxPVIyLz5{M{WzNVkzOTxxYU6=
NGP NGf4T2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTZcGhkTE2VTx?= MoToTWM2OD1yLkW2JO69VQ>? NEP2fZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNVkzOSd-MkO1N|E6OjF:L3G+
IMR32 Mki5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVToXW1wTE2VTx?= M3fST2lEPTB;MD62OkDPxE1? Mn7iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{G5NlEoRjJ|NUOxPVIyRC:jPh?=
LAN5 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzvcZRFVVOR NHrnO5NKSzVyPUGuNFQh|ryP NF\nNWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNVkzOSd-MkO1N|E6OjF:L3G+
RH18 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPEUXNQ MmHRTWM2OD1zLkSyJO69VQ>? NEPBbXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNVkzOSd-MkO1N|E6OjF:L3G+
RH30 Mn3YS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjzbVNSTE2VTx?= M3Pu[mlEPTB;MT6yOUDPxE1? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV|MUmyNUc,OjN3M{G5NlE9N2F-
RH17 MoTqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3v0c2ROW09? MkXhTWM2OD1{LkWxJO69VQ>? NF\q[FU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNVkzOSd-MkO1N|E6OjF:L3G+
RH28 M2nCcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nndGROW09? NUTFZ5AyUUN3ME20MlI5KM7:TR?= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV|MUmyNUc,OjN3M{G5NlE9N2F-
RH36 MnXlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nLOGROW09? M{T2c2lEPTB;NT6zO{DPxE1? NGLtflA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNVkzOSd-MkO1N|E6OjF:L3G+
RH41 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFO4VYhFVVOR NIO4TnRKSzVyPUCuOFgh|ryP NEHLOGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNVkzOSd-MkO1N|E6OjF:L3G+
RD NIDYWmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEC5eXFFVVOR M4mzcWlEPTB;ND6zNkDPxE1? MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV|MUmyNUc,OjN3M{G5NlE9N2F-
TC32 M1\vUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWrEUXNQ Ml\GTWM2OD1|Lki1JO69VQ>? NWS1RWVLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1N|E6OjFpPkKzOVMyQTJzPD;hQi=>
TC71 NGPnUopIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWLvdGRlTE2VTx?= NWnvbotZUUN3ME20MlM{KM7:TR?= M{LyTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUOxPVIyLz5{M{WzNVkzOTxxYU6=
KCNR MWDBdI9xfG:|aYOgRZN{[Xl? MorqNE42NzFwMD:yMlUh|ryP NGPIUXIzPCCq M{js[mROW09? M1H0Uolv\HWlZYOgZY4hcW6lcnXhd4UhcW5iY3HzdIF{\SB|L{egZYN1cX[rdIm= M17iWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUOxPVIyLz5{M{WzNVkzOTxxYU6=
SY5Y Mo\BRZBweHSxc3nzJGF{e2G7 NEHUfG8xNjVxMT6wM|IvPSEQvF2= MV2yOEBp MYfEUXNQ M2TWVIlv\HWlZYOgZY4hcW6lcnXhd4UhcW5iY3HzdIF{\SB|L{egZYN1cX[rdIm= NE\PUm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{WzNVkzOSd-MkO1N|E6OjF:L3G+
Rh18 MVfBdI9xfG:|aYOgRZN{[Xl? MkHuNE42NzFwMD:yMlUh|ryP MYWyOEBp MYXEUXNQ NGXh[JpqdmS3Y3XzJIFvKGmwY4LlZZNmKGmwIHPhd5Bie2ViMz:3JIFkfGm4aYT5 M130V|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUOxPVIyLz5{M{WzNVkzOTxxYU6=
TC32 M4S0NGFxd3C2b4Ppd{BCe3OjeR?= MX[wMlUwOS5yL{KuOUDPxE1? NFLnV4IzPCCq NHvkbWtFVVOR NYrPflhucW6mdXPld{BidiCrbnPy[YF{\SCrbjDjZZNx[XOnIEOvO{Bi[3Srdnn0fS=> MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV|MUmyNUc,OjN3M{G5NlE9N2F-
KCNR MYnGeY5kfGmxbjDBd5NigQ>? MXqwMlUwOS5yL{KuOU82KM7:TR?= M3XSTVI1KGh? MXjEUXNQ Mny4bY5pcWKrdIOgZo91cCCnbnTv[4Vvd3W|IHPvcpN1cXS3dHn2[UBidmRiSVytOk1qdmS3Y3XkJHNVSVR|IHHjeIl3[XSrb36= MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV|MUmyNUc,OjN3M{G5NlE9N2F-
SY5Y MoraSpVv[3Srb36gRZN{[Xl? MV:wMlUwOS5yL{KuOU82KM7:TR?= NGPRRXAzPCCq NFHQdYtFVVOR MmG1bY5pcWKrdIOgZo91cCCnbnTv[4Vvd3W|IHPvcpN1cXS3dHn2[UBidmRiSVytOk1qdmS3Y3XkJHNVSVR|IHHjeIl3[XSrb36= M{LNfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUOxPVIyLz5{M{WzNVkzOTxxYU6=
Rh18 MnHYSpVv[3Srb36gRZN{[Xl? MmH1NE42NzFwMD:yMlUwPSEQvF2= NI\tRY0zPCCq MWHEUXNQ MkPkbY5pcWKrdIOgZo91cCCnbnTv[4Vvd3W|IHPvcpN1cXS3dHn2[UBidmRiSVytOk1qdmS3Y3XkJHNVSVR|IHHjeIl3[XSrb36= MoGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN3M{G5NlEoRjJ|NUOxPVIyRC:jPh?=
TC32 M2O5WmZ2dmO2aX;uJGF{e2G7 Mn\RNE42NzFwMD:yMlUwPSEQvF2= MXyyOEBp M{XsVmROW09? M1K1XolvcGmkaYTzJIJwfGhiZX7kc4dmdm:3czDjc45{fGm2dYTpeoUh[W6mIFnMMVYucW6mdXPl[EBUXEGWMzDhZ5RqfmG2aX;u NYLV[2U{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1N|E6OjFpPkKzOVMyQTJzPD;hQi=>
TPC-1 NYjwTHVTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFP3SXQyKML3TR?= MmfENE01KGR? MVHEUXNQ M1zPO4lvcGmkaYTzJINmdGxiZ4Lve5RpKGGodHXyJFIh\CC2cnXheI1mdnR? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB3NkS5PUc,OjNyNU[0PVk9N2F-
MZ-CRC1  MnLIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXMSGlxOSEEtV2= MkK5NE02KGR? MVnEUXNQ MnXtbY5pcWKrdIOgZ4VtdCCpcn;3eIgh[W[2ZYKgNUBlKHS{ZXH0cYVvfA>? Mlr6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNU[0PVkoRjJ|MEW2OFk6RC:jPh?=
TT  MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1v1c|EhyrWP M3zuPVAuPCCm MWPEUXNQ MlO0bY5pcWKrdIOgZ4VtdCCpcn;3eIgh[W[2ZYKgNUBlKHS{ZXH0cYVvfA>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB3NkS5PUc,OjNyNU[0PVk9N2F-
TPC-1 NYPxUmxvTnWwY4Tpc44hSXO|YYm= MnvZNUDDvU1? M4\oV|czKGh? MWjEUXNQ M1fpO4lv\HWlZYOgS|Eh[myxY3vh[4U> MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB3NkS5PUc,OjNyNU[0PVk9N2F-
MZ-CRC1  NFm4cJFHfW6ldHnvckBCe3OjeR?= NYHvcWhIOSEEtV2= NUfrRllTPzJiaB?= MYHEUXNQ M1f4Volv\HWlZYOgS|Eh[myxY3vh[4U> MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB3NkS5PUc,OjNyNU[0PVk9N2F-
TT  MWPGeY5kfGmxbjDBd5NigQ>? NF;Wb3cyKML3TR?= NXWzfm5mPzJiaB?= MlPGSG1UVw>? Ml[xbY5lfWOnczDHNUBjdG:la3Hn[S=> MmTMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNU[0PVkoRjJ|MEW2OFk6RC:jPh?=
MZ-CRC1  NWPsSFFISXCxcITvd4l{KEG|c3H5 NV65N2g{OSEEtV2= MUK0PEBp MXvEUXNQ MoXWbY5lfWOnczDhdI9xfG:|aYO= MlH2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNU[0PVkoRjJ|MEW2OFk6RC:jPh?=
TT  MoHLRZBweHSxc3nzJGF{e2G7 MWixJOK2VQ>? MoTmOFghcA>? M2XLNGROW09? NGrHZ2tqdmS3Y3XzJIFxd3C2b4Ppdy=> NY[4SmlmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOVY1QTlpPkKzNFU3PDl7PD;hQi=>
HD-LM2 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnHLO|LjiImq NWf3[mJiTE2VTx?= NHzSe3ZKSzVyPUeuPFQ1KM7:TR?= NXrUW5VXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlkxQTRpPkKyPFI6ODl2PD;hQi=>
L-428 NGXpUZJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIexcG04OuLCiXi= MoD0SG1UVw>? M4Trc2lEPTB;Nz65OFch|ryP MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{OUC5OEc,OjJ6MkmwPVQ9N2F-
KM-H2 M3TlTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfLO|LjiImq M3yy[mROW09? MVLJR|UxRTFwM{C4JO69VQ>? NYG2PGxORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlkxQTRpPkKyPFI6ODl2PD;hQi=>
L-540 M1LQWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7vd3ZDPzMkgJno M2DnWGROW09? MoGxTWM2OD16LkKxOkDPxE1? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjh{OUC5OEc,OjJ6MkmwPVQ9N2F-
HD-LM2 NVPHT5J2TnWwY4Tpc44hSXO|YYm= M3;C[VAvOS9yLkWvNU82KM7:TR?= NX:2T3ZHPzMkgJno NF\pNYdFVVOR MWTpcohq[mm2czDTWGFVOyxiU2TBWFUh[W6mIGPURXQ3KHCqb4PwbI9zgWyjdHnvci=> M1H3eVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK5NFk1Lz5{MkiyPVA6PDxxYU6=
L-428 Mk\mSpVv[3Srb36gRZN{[Xl? Ml22NE4yNzBwNT:xM|Uh|ryP NF\sS|g4OuLCiXi= NHrMbnBFVVOR M3;t[IlvcGmkaYTzJHNVSVR|LDDTWGFVPSCjbnSgV3RCXDZicHjvd5Bpd3K7bHH0bY9v NXfx[YU5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlkxQTRpPkKyPFI6ODl2PD;hQi=>
KM-H2 MnPKSpVv[3Srb36gRZN{[Xl? NWDz[lVjOC5zL{CuOU8yNzVizszN M1jZVFcz6oDLaB?= MXjEUXNQ MVTpcohq[mm2czDTWGFVOyxiU2TBWFUh[W6mIGPURXQ3KHCqb4PwbI9zgWyjdHnvci=> NGDKbok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyPVA6PCd-MkK4NlkxQTR:L3G+
L-540 NWj3SnhmTnWwY4Tpc44hSXO|YYm= MV[wMlEwOC53L{GvOUDPxE1? NYLYTmx5PzMkgJno NGPiSZlFVVOR NHvFd3ZqdmirYnn0d{BUXEGWMzygV3RCXDViYX7kJHNVSVR4IIDoc5NxcG:{eXzheIlwdg>? NF7uUZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkiyPVA6PCd-MkK4NlkxQTR:L3G+
HD-LM2 Mly5RZBweHSxc3nzJGF{e2G7 NHPyd|gyNzVizszN M{j5UFcz6oDLaB?= NYnkcpdYTE2VTx?= MnHHbY5lfWOnczDhdI9xfG:|aYO= M4DUelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK5NFk1Lz5{MkiyPVA6PDxxYU6=
L-428 NYK0OIkxSXCxcITvd4l{KEG|c3H5 NXvPSI1bOS93IN88US=> M1zScVcz6oDLaB?= NUnSVGNiTE2VTx?= MXnpcoR2[2W|IHHwc5B1d3Orcx?= M{XOU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK5NFk1Lz5{MkiyPVA6PDxxYU6=
KM-H2 NHTvXo5CeG:ydH;zbZMhSXO|YYm= M3;WelEwPSEQvF2= NFPyR404OuLCiXi= MkDLSG1UVw>? M1W5Oolv\HWlZYOgZZBweHSxc3nz NX:2WVZMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK4NlkxQTRpPkKyPFI6ODl2PD;hQi=>
L-540 NULWb3BqSXCxcITvd4l{KEG|c3H5 MkS2NU82KM7:TR?= MXG3NwKBkWh? M3nQdGROW09? MVvpcoR2[2W|IHHwc5B1d3Orcx?= M1Lm[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{OEK5NFk1Lz5{MkiyPVA6PDxxYU6=
U251-MG NEH3e3ZHfW6ldHnvckBCe3OjeR?= NV;mWHp6OSEEtV2= NUfvR5oyOC1zNjDo Mm[zbY5pcWKrdIOgZ49ve3SrdIX0bZZmKFOWQWStN{BidmRiSlHLNkBi[3SrdnH0bY9v NFf1eHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCyO|Y6OSd-MkKwNlc3QTF:L3G+
U87-MG MWrGeY5kfGmxbjDBd5NigQ>? NFTjbXgyKML3TR?= MknYNE0yPiCq NFf3VodqdmirYnn0d{Bkd26|dHn0eZRqfmViU2TBWE0{KGGwZDDKRWszKGGldHn2ZZRqd25? M1vl[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEK3OlkyLz5{MkCyO|Y6OTxxYU6=
4C8 MVjGeY5kfGmxbjDBd5NigQ>? MmDwNUDDvU1? MW[wMVE3KGh? MlrtbY5pcWKrdIOgZ49ve3SrdIX0bZZmKFOWQWStN{BidmRiSlHLNkBi[3SrdnH0bY9v NGfhc5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkCyO|Y6OSd-MkKwNlc3QTF:L3G+
U251-MG MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3m2Z|EwOTBiwsXN NEfnUGEzPC92OD:3NkBp M4\ydYlvcGmkaYTzJINmdGxicILvcIln\XKjdHnvckBifCCjIHPvcoNmdnS{YYTpc44hd2ZiMUCgxtVO MojlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJyMke2PVEoRjJ{MEK3OlkyRC:jPh?=
U87-MG NVXO[Xd5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWqxM|ExKML3TR?= NWL6NmxsOjRxNEivO|IhcA>? NUSwXXRbcW6qaXLpeJMh[2WubDDwdo9tcW[ncnH0bY9vKGG2IHGgZ49v[2WwdILheIlwdiCxZjCxNEDDvU1? M2nMTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{MEK3OlkyLz5{MkCyO|Y6OTxxYU6=
4C8 M3v6W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjyZ3N5OS9zMDFCuW0> MlKwNlQwPDhxN{KgbC=> NXr3[o1WcW6qaXLpeJMh[2WubDDwdo9tcW[ncnH0bY9vKGG2IHGgZ49v[2WwdILheIlwdiCxZjCxNEDDvU1? NXHWR2YxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKwNlc3QTFpPkKyNFI4PjlzPD;hQi=>
U266 NGrCT2xCeG:ydH;zbZMhSXO|YYm= MmTlNE42NTJizszN Mlf5OFgwPzJiaB?= M1K0dYlv\HWlZYOgZZBweHSxc3nzJIRwe2ViZHXw[Y5l\W62bIm= MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF4NEWxO{c,OjFzNkS1NVc9N2F-
Kms.11 NYmyTXNlSXCxcITvd4l{KEG|c3H5 M2PFbVAvPS1{IN88US=> NHrVcnI1QC95MjDo M3znbolv\HWlZYOgZZBweHSxc3nzJIRwe2ViZHXw[Y5l\W62bIm= NIPL[VU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUG2OFUyPyd-MkGxOlQ2OTd:L3G+
8226 MXjBdI9xfG:|aYOgRZN{[Xl? MkLqNE42NTJizszN NVPoc|ZOPDhxN{KgbC=> M3\xOIlv\HWlZYOgZZBweHSxc3nzJIRwe2ViZHXw[Y5l\W62bIm= M4\KUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMU[0OVE4Lz5{MUG2OFUyPzxxYU6=
SET2 M2G1b2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NWT2bm9FPDhiaILz NXTDUHpCT3Kxd4ToJIlvcGmkaYTpc44hd2ZiaIXtZY4hW0WWMjDj[YxteyCneIDy[ZN{cW6pIFrhb|IhXjZzN1[gcZV1[W62IHHmeIVzKDR6IHjyd{BjgSCPVGOgZZN{[XluIFfJOVAhRSByLkCxOkDPxE1w MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF|OEK0Okc,OjFzM{iyOFY9N2F-
SET2 M4juZ2Z2dmO2aX;uJIF{e2G7 MWexJIhz MXPJcohq[mm2aX;uJI9nKFO2YYSzJJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDTSXQzKGOnbHzzJIFnfGW{IEGgbJIh[nliV3XzeIVzdiCkbH;0eIlv\yxiSVO1NEA:KDBwMEKzJO69VS5? NXXvW|V2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxN|gzPDZpPkKxNVM5OjR4PD;hQi=>
SET2 NYDLfnBtTnWwY4Tpc44h[XO|YYm= M2HTfFEhcHJ? MV;Jcohq[mm2aX;uJI9nKFO2YYS1JJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDTSXQzKGOnbHzzJIFnfGW{IEGgbJIh[nliV3XzeIVzdiCkbH;0eIlv\yxiSVO1NEA:KDBwMEK1JO69VS5? NFnGZ4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGzPFI1Pid-MkGxN|gzPDZ:L3G+
HEL M{D2[mZ2dmO2aX;uJIF{e2G7 M1m3d|EhcHJ? M2nQUGlvcGmkaYTpc44hd2ZiU4TheFUheGixc4Doc5J6dGG2aX;uJIlvKGi3bXHuJGhGVCClZXzsd{Bi\nSncjCxJIhzKGK7IGfld5Rmem5iYnzveJRqdmduIFnDOVAhRSByLkC0NUDPxE1w NX7q[3JQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxN|gzPDZpPkKxNVM5OjR4PD;hQi=>
Ba/F3 MXTGeY5kfGmxbjDhd5NigQ>? M1LGPWlvcGmkaYTpc44hd2ZiU4TheFUheGixc4Doc5J6dGG2aX;uJIlvKG2xdYPlJGJiN0Z|IHPlcIx{KGW6cILld5NqdmdiVFXMMWpiczJuIFnDOVAhRSByLkC0OkDPxE1w M2rKZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUO4NlQ3Lz5{MUGzPFI1PjxxYU6=
UKE1 M1z4O2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MoO2OFghcHK| MXzHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDVT2UyKGOnbHzzJIV5eHKnc4PpcochUmGtMjDWOlE4TiCvdYThcpQh[W[2ZYKgOFghcHK|IHL5JG1VWyCjc4PhfUwhT0l3MDC9JFAvODV2IN88UU4> M1;BfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUO4NlQ3Lz5{MUGzPFI1PjxxYU6=
BA/F3 MlXaS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? MkfIOFghcHK| NHr6eHpIem:5dHigbY5pcWKrdHnvckBw\iCvb4Xz[UBDSS:IMzDj[YxteyCneIDy[ZN{cW6pIGTlcE1LSUt{IHvpcoF{\SCjZoTldkA1QCCqcoOgZpkhVVSVIHHzd4F6NCCJSUWwJF0hOC5yNjFOwG0v MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF|OEK0Okc,OjFzM{iyOFY9N2F-
HEL M3XUNGZ2dmO2aX;uJIF{e2G7 MnfJNUBpeg>? NIPR[mxKdmirYnn0bY9vKG:oIGP0ZZQ{KHCqb4PwbI9zgWyjdHnvckBqdiCqdX3hckBJTUxiY3XscJMh[W[2ZYKgNUBpeiCkeTDX[ZN1\XKwIHLsc5R1cW6pLDDJR|UxKD1iMD6wPEDPxE1w NGD1clc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGzPFI1Pid-MkGxN|gzPDZ:L3G+
HEL M33xS2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NELxfpQ1QCCqcoO= M{n6dWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJGhGVCClZXzsd{BmgHC{ZYPzbY5oKEqja{KgWlYyP0ZibYX0ZY51KGGodHXyJFQ5KGi{czDifUBOXFNiYYPzZZktKEeLNUCgQUAxNjN7IN88UU4> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF|OEK0Okc,OjFzM{iyOFY9N2F-
BA/F3 NVHpfHhZT3Kxd4ToJIlvcGmkaYTpc44h[XO|YYm= MU[0PEBpenN? M1jneGdzd3e2aDDpcohq[mm2aX;uJI9nKG2xdYPlJGJCN0Z|IHPlcIx{KGW6cILld5NqdmdiVHXsMWpCUzFia3nuZZNmKGGodHXyJFQ5KGi{czDifUBOXFNiYYPzZZktKEeLNUCgQUAxNjdizszNMi=> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTF|OEK0Okc,OjFzM{iyOFY9N2F-
BA/F3 M33WXGdzd3e2aDDpcohq[mm2aX;uJIF{e2G7 NUTVUW4xPDhiaILz Mo\4S5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgRmEwTjNiY3XscJMh\XiycnXzd4lv\yCWZXytWJlsOiCtaX7hd4Uh[W[2ZYKgOFghcHK|IHL5JG1VWyCjc4PhfUwhT0l3MDC9JFIh|ryPLh?= NIDnepQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUGzPFI1Pid-MkGxN|gzPDZ:L3G+
BA/F3 MVPHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? NH7BbIY1QCCqcoO= MmD5S5Jwf3SqIHnubIljcXSrb36gc4YhdW:3c3WgRmEwTjNiY3XscJMh\XiycnXzd4lv\yCWZXytTmFMOyCtaX7hd4Uh[W[2ZYKgOFghcHK|IHL5JG1VWyCjc4PhfUwhT0l3MDC9JFIvOSEQvF2u NULpTVd1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxN|gzPDZpPkKxNVM5OjR4PD;hQi=>
UKE1 MVHGeY5kfGmxbjDhd5NigQ>? M3raOVEhcHJ? MVvJcohq[mm2aX;uJI9nKFO2YYS1JJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDVT2UyKGOnbHzzJIFnfGW{IEGgbJIh[nliV3XzeIVzdiCkbH;0eIlv\w>? M4W2cVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMUO4NlQ3Lz5{MUGzPFI1PjxxYU6=
SW620 MYTD[YxtKGO7Y3zlJIF{e2G7 M1n4O|MvOyC3TR?= NH;3PHMzPCCqcoO= MXzD[YxtKGO7Y3zlJIFzemW|dDDpckBpfW2jbjDTW|YzOCClZXzsd{Bie3Onc4Pl[EBieyCjY3P1cZVt[XSrb36gZZQhTzJxTTDwbIF{\SCjdDCzMlMhfU1iYX\0[ZIhOjRiaILzJIJ6KG[ub4egZ5l1d22ndIL5 MkjiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzM{iyOFYoRjJzMUO4NlQ3RC:jPh?=
HEL MoXySpVv[3Srb36gZZN{[Xl? NXzIcFJ1OC5|IIXN M4Szc|UhdWmwcx?= NV62SnVqUW6qaXLpeIlwdiCxZjDTeIF1PSCyaH;zdIhwenmuYYTpc44hcW5iaIXtZY4hUEWOIHPlcIx{KGG2IECuN{B2VSCjZoTldkA2KG2rboOgZpkhX2W|dHXyckBjdG:2dHnu[y=> MmjZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzM{iyOFYoRjJzMUO4NlQ3RC:jPh?=
HEL MWLGeY5kfGmxbjDhd5NigQ>? NV[5UFNGOC5|IIXN M1jrVlMxKHSxIE[wJI1qdnN? Mn7ZTY5pcWKrdHnvckBw\iCVdHH0OUBxcG:|cHjvdplt[XSrb36gbY4hcHWvYX6gTGVNKGOnbHzzJIF1KDBwMzD1UUBi\nSncjCzNEB1dyB4MDDtbY5{KGK7IGfld5Rmem5iYnzveJRqdmd? Mk[yQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFzM{iyOFYoRjJzMUO4NlQ3RC:jPh?=
BA/F3 NYjKUnNjSW62aYT1cY9zKGG|c3H5 NXXjTFhMOzBibXevb4c> M4jpW|EzKGi{cx?= MXjBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDtc5V{\SCEQT;GN{Bk\WyuczDlfJBz\XO|aX7nJHRmdC2MQVuyJJhmdm:pcnHmeIVlKGmwIF7DdkBud3W|ZTDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hd2ZicHjvd5Bpd3K7bHH0[YQhW3SjdEWgcIV3\WxiaX6gd5Bt\WWwIHH0JFMxKG2pL3vnMEBxdyCjZoTldkAyOiCqcoOgZpkhX2W|dHXyckBjdG:2dHnu[y=> NUTiT5hCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxN|gzPDZpPkKxNVM5OjR4PD;hQi=>
HEL MX3GeY5kfGmxbjDhd5NigQ>? NWnK[GltOC5|IIXN M2TYOlUhdWmwcx?= M{\Z[GlvcGmkaYTpc44hd2ZiU4TheFMheGixc4Doc5J6dGG2aX;uJIlvKGi3bXHuJGhGVCClZXzsd{BifCByLkOgeW0h[W[2ZYKgOUBucW6|IHL5JHdme3Sncn6gZoxwfHSrbne= NUXkN3VWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxN|gzPDZpPkKxNVM5OjR4PD;hQi=>
HEL NUfwd284TnWwY4Tpc44h[XO|YYm= NGL4VJYxNjNidV2= NGqxZ2g{OCC2bzC2NEBucW6| MV\Jcohq[mm2aX;uJI9nKFO2YYSzJJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDISWwh[2WubIOgZZQhOC5|IIXNJIFnfGW{IEOwJJRwKDZyIH3pcpMh[nliV3XzeIVzdiCkbH;0eIlv\w>? NWTCUGc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGxN|gzPDZpPkKxNVM5OjR4PD;hQi=>
Hs578T NY\SN3gzTnWwY4Tpc44h[XO|YYm= NHS0eGsyKHWP M{XZT|I1KGi{cx?= MWPJcohq[mm2aX;uJI9nKFOWQWSzR{1u\WSrYYTl[EB1emGwc3PybZB1cW:wIHnuJIh2dWGwIFjzOVc5XCClZXzsd{BifCBzIIXNJIFnfGW{IEK0JIhzeyCkeTDseYNq\mW{YYPlJJJmeG:{dHXyJIdmdmViYYPzZZk> NWXrTIp7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5O|gyOTJpPkK0PVc5OTF{PD;hQi=>
NCI-H1975 MoTCSpVv[3Srb36gZZN{[Xl? M2mzRlAvODB|IITvJFMhfU1? NVHFPI45OiCqcoO= MkW0TY5pcWKrdHnvckBw\iCMQVuxJIlvKGi3bXHuJG5EUS2KMUm3OUBk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iU2TBWFMheGixc4Doc5J6dGG2aX;uJIF1KDBwMECzJJRwKDNidV2gZYZ1\XJiMjDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz NYTJdm5FRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMk[2NVQ1ODhpPkK2OlE1PDB6PD;hQi=>
HEL 92.1.7 M2LzWmZ2dmO2aX;uJIF{e2G7 NETOcpAzKGi{cx?= MXjJcohq[mm2aX;uJI9nKEqDS{KgbY4hcHWvYX6gTGVNKDl{LkGuO{Bk\WyuczDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iU2TBWFUheGixc4Doc5J6dGG2aX;uJIFnfGW{IEKgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NF;pO3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{Nk[xOFQxQCd-Mk[2NVQ1ODh:L3G+
DAOY MmK4dWhVWyCjc4PhfS=> MVTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhTEGRWTDj[Yxtew>? NVH5[mpFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
SJ-GBM2 NYPDbnRJeUiWUzDhd5NigQ>? NUeyTlM5eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPKMWdDVTJiY3XscJM> NFfKNVM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
A673 MXLxTHRUKGG|c3H5 NEXPXndyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucx?= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC M{HWTZFJXFNiYYPzZZk> M{PBdZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSz3OMW1EKGOnbHzz MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
BT-37 M4XKTZFJXFNiYYPzZZk> NV\kUIF2eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IFLUMVM4KGOnbHzz MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB-EBc1 M3:4[JFJXFNiYYPzZZk> MoDIdWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohWHKrbXHyfUB{[3KnZX6g[o9zKE6ELVXCZ|Eh[2WubIO= NXPEe|ViRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Saos-2 MUjxTHRUKGG|c3H5 NUHhSGxPeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IGPhc5MuOiClZXzsdy=> Ml;RQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-SH NFr2c2FyUFSVIHHzd4F6 MYPxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3TTEBk\Wyucx?= M4Xz[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB1643 MlHWdWhVWyCjc4PhfS=> NF7HdoxyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz NGD0bIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
BT-12 MlnndWhVWyCjc4PhfS=> MYHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhSlRvMUKgZ4VtdHN? MlX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
OHS-50 NVrWZnN5eUiWUzDhd5NigQ>? NYiySZF1eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiUILpcYFzgSC|Y4Ll[Y4h\m:{IF;IV{02OCClZXzsdy=> M{n5UVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD M3O2eZFJXFNiYYPzZZk> MV;xTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWkRiY3XscJM> NVPiN|hrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
MG 63 (6-TG R) Mn7ZdWhVWyCjc4PhfS=> M{jObZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCPRzC2N{ApPi2WRzDSLUBk\Wyucx?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh41 MmfadWhVWyCjc4PhfS=> MUTxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhWmh2MTDj[Yxtew>? NVnMSYh5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
Assay
Methods Test Index PMID
Western blot p-STAT3 / STAT3 / p-JAK2 / JAK2 p-STAT1 / STAT1 / p-STAT5 / STAT5 / p-STAT6 / STAT6 p-ERK / ERK / p-p38 / p38 / SOCS3 / p-SHP2 / SHP2 22027691 22829094
Immunofluorescence pSTAT5 30140255
Growth inhibition assay Cell proliferation Cell viability 22027691 25855603
体内研究 AZD1480作用于人类实体瘤模型和多发性骨髓瘤模型,抑制 STAT3磷酸化。[1]在体内, AZD1480抑制皮下移植瘤的生长,且通过抑制STAT3活性提高携带颅内恶性胶质瘤(GBM)的鼠寿命,说明AZD1480抑制JAK/STAT3通路的药理学可以用于研究治疗携带颅内恶性胶质瘤的患者。[3]AZD1480抑制骨髓细胞的肺浸润和肺转移瘤。AZD1480作用于人类移植瘤模型降低血管生成和代谢。[4]AZD1480抑制含有STAT活性的人类实体瘤的生长。[5]

推荐的实验操作(此推荐来自于公开的文献所以Selleck并不保证其有效性)

激酶实验:[5]
  • 激酶实验:

    在有buffer(包括50 mM HEPES pH 为7.3, 1 mM DTT, 0.01% Tween-20, 50 µg/ml BSA,及10 mM MgCl2)的条件下,使用重组Jak1, Jak2, 或Jak3进行AZD1480抑制效果研究。在昆虫细胞中,Jak3酶表达为氮端GST融合蛋白,且通过谷胱甘肽亲和及尺寸排除色谱来纯化Jak3酶。加入AZD1480 (10点剂量反应, 重复三次, 8.3 µM 到0.3 nM ),使用1.5 µM 肽底物(Jak1:FITC-C6-KKHTDDGYMPMSPGVA-NH2, Jak2和Jak3: FAM-SRCtide),及各自ATP Km (Jak1: 55 µM, Jak2: 15 µM, Jak3: 3 µM),ATP浓度为5 mM,进行酶实验。分离磷酸化和未磷酸化的肽段,使用Caliper LC3000系统计算抑制百分率。

细胞实验:[4]
  • Cell lines: Renca或786-O细胞,鼠内皮细胞和脾CD11b+/c−骨髓细胞, HUVECs
  • Concentrations: 1 μM
  • Incubation Time: 48或24小时
  • Method: Renca或786-O细胞悬浮在含5%FBS的DMEM培养基中,按每孔5×103个细胞接种在96孔板上,使细胞粘附,实验组用AZD1480,对照组用DMSO,处理48小时。通过MTS实验测定细胞活力。使用Mikrotek Laborsysteme在490nm处测定吸光值。鼠内皮细胞和脾CD11b+/c− 骨髓细胞从携带肿瘤的鼠中富集得到,培养在含5% FBS的RPMI-1640培养基上。HUVECs 培养在胶原1包被的含完全培养基的板上。用DMSO和不同剂量AZD1480处理所有细胞,持续24小时。手动计算细胞数而测定细胞活力。所有实验重复三次。
动物实验:[4]
  • Animal Models: 雌性BALB/c和无胸腺鼠(NCR− nu/nu) (7-8周大)
  • Dosages: 每天一次,剂量为50 mg/kg;或者每天两次,剂量为30 mg/kg
  • Administration: 口服饲喂

溶解度(25°C)

体外

体内

从左到右依次将纯溶剂加入产品,现配现用(数据来自Selleck实验检测而非文献):
30% PEG400+0.5% Tween80+5% propylene glycol

5 mg/mL

* 溶解度检测是由Selleck技术部门检测的,可能会和文献中提供的溶解度有所差异,这是由于生产工艺和批次不同产生的正常现象。请按照顺序依次加入各个纯溶剂。

化学数据

分子量 348.77
化学式

C14H14ClFN8

CAS号 935666-88-9
储存条件 3年 -20°C 粉状
2年 -80°C 溶于溶剂

动物体内配方计算器(澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)

mg/kg g μL

第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系Selleck为您提供正确的澄清溶液配方)

% DMSO % % Tween 80 % ddH2O
%DMSO %

计算结果:

工作液浓度: mg/ml;

DMSO母液配制方法: mg 药物溶于μL DMSO溶液(母液浓度mg/mL,:如该浓度超过该批次药物DMSO溶解度,请先联系Selleck);

体内配方配制方法:μL DMSO母液,加入μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入μL ddH2O,混匀澄清。

体内配方配制方法:μL DMSO母液,加入μL Corn oil,混匀澄清。

注意:1. 首先保证母液是澄清的;
2.一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。

摩尔浓度计算器

质量 浓度 体积 分子量

临床试验信息

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01219543 Terminated Drug: AZD1480 Daily|Drug: AZD1480 BID Solid Tumour|Advanced Solid Malignancies|Child-Pugh A to B7 Advanced Hepatocellular Carcinoma|EGFR and/or ROS Mutant NSCLC|Lung Metastasis Carcinoma|Gastric Cancer AstraZeneca November 2010 Phase 1
NCT01112397 Terminated Drug: AZD1480 Solid Malignancies AstraZeneca April 2010 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

技术支持

在订购、运输、储存和使用我们的产品的任何阶段,您遇到的任何问题,均可以通过拨打我们的热线电话400-668-6834,或者技术支持邮箱tech@selleck.cn,直接联系到我们。我们会在24小时内尽快联系您。

操作手册

如果有其他问题,请给我们留言。

* 必填项

请输入您的姓名
请输入您的邮箱地址 请输入一个有效的邮箱地址
请写点东西给我们
Tags: buy AZD1480|AZD1480 ic50|AZD1480 price|AZD1480 cost|AZD1480 solubility dmso|AZD1480 purchase|AZD1480 manufacturer|AZD1480 research buy|AZD1480 order|AZD1480 mouse|AZD1480 chemical structure|AZD1480 mw|AZD1480 molecular weight|AZD1480 datasheet|AZD1480 supplier|AZD1480 in vitro|AZD1480 cell line|AZD1480 concentration|AZD1480 nmr|AZD1480 in vivo|AZD1480 clinical trial|AZD1480 inhibitor|AZD1480 JAK/STAT inhibitor